Status
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Gene information
General information
TC015823 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 8951912..8961520
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC015823
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02519

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva segment partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015823 RA
      MYESPNSLE DLCLDTICD NILIYVKLD HTNAGKRYK 
FADPDLFLF KELSEKLLG KFLEKNILC DALMFIFSE 
KNTKLSTVK LKNCKVTQN GLEILKQHK ILDLECVNL 
KNVCIGNII DCLNEHSIR NMTNVNFAK CSFIDVQRH 
SLMVKITQF KNLRSLNLA YTELNQHCL NMICEDLKL 
LEKLDISGT GTGIKDLSP LRQLLNLTS LAISDLSQS 
ANYVSTLTH LSTLRHLDV SVFNEKLDH LTTSNVVKL 
LDDVTTLPH LVSLDVSGW RDFIPTETL LKYVETHPK 
LEFLGIVLT DCTFDSLFS STNQWPNLT IAGLGNENQ 
IKVTLKKYR ERSNYVQKA LYHLFQLTN LSPDARPDM 
FNLVLPVMA AHSNKFGVQ MAATACLYN LTRGDLSKK 
IHPKSLSRG VNLTLYAME CFPDEFQLQ KNSLLTLCS 
DTILQEINF DRFRCAKLV LDALCTFED VNMNRMAVA 
ICSILAAKV STEETSELG ARPVYMRKL LAMVQNRVE 
SGVSDITLK FTLSALWNL TDESAATCT VFLEQGGAY 
LFLKVLKTF KDDSAIETK VLGLLNNIA EVVKLRHSL 
MMDPLMNEL FVLLKSENI DVSYFAAGI VAHLASDGA 
EQWTVGSHT REEMLGELK NAVSQWKVP DSEMVAYRS 
FRPFFSLLR IEMDYQVQL WAVWAIHHV CTKNPKRYC 
VMLQDENGH LLLSDLINY TDSHPDIKS ISSQILETV 
AGHV
    CDS sequence
    • >TC015823 RA
      atgtacgaa agtccgaat tctttggaa gatttgtgt 
ttggacacg atttgtgac aatattctc atttacgtg 
aaactcgat catacaaat gcgggaaaa cgttataaa 
ttcgccgat cctgatttg ttcctcttc aaggaactg 
tcagagaaa cttttaggg aaattcctc gagaagaat 
atcctttgt gacgcctta atgtttata tttagtgag 
aagaacacg aaactgagc actgttaaa ttaaaaaat 
tgcaaagtg acacagaac ggacttgag atacttaaa 
cagcacaag attttagat ttagaatgt gtcaatttg 
aaaaatgtg tgtatagga aatattatt gattgtctc 
aatgaacac tcaattaga aacatgacg aatgtgaat 
ttcgctaaa tgctcgttt atcgacgtg cagaggcac 
agtttgatg gtgaaaatt acacaattc aaaaatcta 
cgctctctc aatttggcg tacactgaa ctcaaccaa 
cactgctta aatatgatt tgtgaggac ctgaaacta 
ctggaaaaa ctcgacatt tcgggaact ggcacgggg 
atcaaggac cttagccct ctgaggcaa ctcctcaac 
ttgacgagt ctcgcaatc agcgacttg tcgcagtca 
gctaactac gtctcgacg ctcacccac ctgtccacc 
ttacggcac ctcgacgtg agcgttttc aacgaaaaa 
ctcgaccac ttgaccacc tccaacgtc gtcaaactc 
ctcgacgac gtcacaacg ctcccccat ttagtctcg 
ctcgatgta agcggctgg cgtgacttt atcccgact 
gaaaccctc cttaaatac gttgaaact caccccaag 
ttagaattt ctaggtatc gtccttacc gactgtacg 
ttcgattcg ctgttcagt tctaccaac cagtggcca 
aatttgaca atcgccggt ttgggaaac gaaaatcag 
attaaagtc acgttaaag aagtataga gaacggtcg 
aattatgta caaaaagcg ctctatcac ctgtttcaa 
ttgacgaat ttatcgccg gatgcgcgt cccgacatg 
tttaatttg gtacttccg gtcatggcg gcacattcg 
aataagttt ggcgtgcaa atggccgcc actgcgtgt 
ctgtataat ctaacacgg ggtgatttg agtaagaaa 
atccatcca aagtcgttg agtcgaggg gtgaatctc 
accctgtat gcgatggag tgtttcccc gatgagttt 
cagttgcag aaaaattca cttttgacg ctttgcagc 
gacaccata ttgcaggag ataaatttt gatcgtttt 
cggtgcgca aagttagtt ttggacgcc ctttgtacg 
ttcgaagac gtgaatatg aatcggatg gcggttgca 
atttgtagt attttggcg gcgaaagtt agcaccgaa 
gaaacgtcc gaattgggg gcacgcccc gtttacatg 
cgtaaacta ctcgcaatg gttcagaat cgggtcgag 
tcgggcgtt tccgacatc actcttaag ttcacactc 
agcgccctc tggaacctt accgatgaa agtgccgcc 
acctgtacg gtttttctg gagcaaggt ggcgcctac 
ttgtttttg aaagttttg aaaacgttt aaagacgat 
agtgcaatt gagacgaaa gttttaggt ttgttgaat 
aatatagcc gaagttgtg aagctgagg catagtttg 
atgatggat ccgttgatg aatgagttg tttgttttg 
ttaaaatcg gaaaatatt gatgttagt tattttgcg 
gccgggatt gtggcacat ttagcttca gatggggct 
gagcaatgg acggtgggg agtcataca agggaggag 
atgttgggg gagttgaaa aatgcggtc tcgcaatgg 
aaagtgcca gatagtgaa atggtggcc tatcgcagc 
tttaggccg tttttttca ctgttgagg atcgagatg 
gactatcag gtgcagctg tgggcggtg tgggccata 
catcacgtc tgtaccaaa aatccaaaa cggtactgc 
gtcatgtta caagacgaa aacggccat ctcctcctc 
agcgacttg atcaattac accgactcc catcccgat 
attaaatca atctcgtct cagatcctc gaaacggtg 
gctggccac gtttga
    mRNA sequence
    • >TC015823 RA
      gccgtcttt aaattgtga tagttttga aatgtcagg 
tgtcacaac tttgacagt tcgaaatga aaatgagtt 
tgacgataa attcttgat attccgtgt aacggttcg 
attgcggca atgatcacc tcataaaga aaataaaag 
cccaatcga agaaaaagt gaggttagg tgaaaatgt 
acgaaagtc cgaattctt tggaagatt tgtgtttgg 
acacgattt gtgacaata ttctcattt acgtgaaac 
tcgatcata caaatgcgg gaaaacgtt ataaattcg 
ccgatcctg atttgttcc tcttcaagg aactgtcag 
agaaacttt tagggaaat tcctcgaga agaatatcc 
tttgtgacg ccttaatgt ttatattta gtgagaaga 
acacgaaac tgagcactg ttaaattaa aaaattgca 
aagtgacac agaacggac ttgagatac ttaaacagc 
acaagattt tagatttag aatgtgtca atttgaaaa 
atgtgtgta taggaaata ttattgatt gtctcaatg 
aacactcaa ttagaaaca tgacgaatg tgaatttcg 
ctaaatgct cgtttatcg acgtgcaga ggcacagtt 
tgatggtga aaattacac aattcaaaa atctacgct 
ctctcaatt tggcgtaca ctgaactca accaacact 
gcttaaata tgatttgtg aggacctga aactactgg 
aaaaactcg acatttcgg gaactggca cggggatca 
aggacctta gccctctga ggcaactcc tcaacttga 
cgagtctcg caatcagcg acttgtcgc agtcagcta 
actacgtct cgacgctca cccacctgt ccaccttac 
ggcacctcg acgtgagcg ttttcaacg aaaaactcg 
accacttga ccacctcca acgtcgtca aactcctcg 
acgacgtca caacgctcc cccatttag tctcgctcg 
atgtaagcg gctggcgtg actttatcc cgactgaaa 
ccctcctta aatacgttg aaactcacc ccaagttag 
aatttctag gtatcgtcc ttaccgact gtacgttcg 
attcgctgt tcagttcta ccaaccagt ggccaaatt 
tgacaatcg ccggtttgg gaaacgaaa atcagatta 
aagtcacgt taaagaagt atagagaac ggtcgaatt 
atgtacaaa aagcgctct atcacctgt ttcaattga 
cgaatttat cgccggatg cgcgtcccg acatgttta 
atttggtac ttccggtca tggcggcac attcgaata 
agtttggcg tgcaaatgg ccgccactg cgtgtctgt 
ataatctaa cacggggtg atttgagta agaaaatcc 
atccaaagt cgttgagtc gaggggtga atctcaccc 
tgtatgcga tggagtgtt tccccgatg agtttcagt 
tgcagaaaa attcacttt tgacgcttt gcagcgaca 
ccatattgc aggagataa attttgatc gttttcggt 
gcgcaaagt tagttttgg acgcccttt gtacgttcg 
aagacgtga atatgaatc ggatggcgg ttgcaattt 
gtagtattt tggcggcga aagttagca ccgaagaaa 
cgtccgaat tgggggcac gccccgttt acatgcgta 
aactactcg caatggttc agaatcggg tcgagtcgg 
gcgtttccg acatcactc ttaagttca cactcagcg 
ccctctgga accttaccg atgaaagtg ccgccacct 
gtacggttt ttctggagc aaggtggcg cctacttgt 
ttttgaaag ttttgaaaa cgtttaaag acgatagtg 
caattgaga cgaaagttt taggtttgt tgaataata 
tagccgaag ttgtgaagc tgaggcata gtttgatga 
tggatccgt tgatgaatg agttgtttg ttttgttaa 
aatcggaaa atattgatg ttagttatt ttgcggccg 
ggattgtgg cacatttag cttcagatg gggctgagc 
aatggacgg tggggagtc atacaaggg aggagatgt 
tgggggagt tgaaaaatg cggtctcgc aatggaaag 
tgccagata gtgaaatgg tggcctatc gcagcttta 
ggccgtttt tttcactgt tgaggatcg agatggact 
atcaggtgc agctgtggg cggtgtggg ccatacatc 
acgtctgta ccaaaaatc caaaacggt actgcgtca 
tgttacaag acgaaaacg gccatctcc tcctcagcg 
acttgatca attacaccg actcccatc ccgatatta 
aatcaatct cgtctcaga tcctcgaaa cggtggctg 
gccacgttt gaagatctc cagcagcat tattgtgat 
ttacccata attctcagt tacttttta cttaggatt 
gttaccact gtgagataa ttaagttgc gcccaactc 
cccccgttg tatattttt attacgttg tcgattggt 
tgtgtttta agacgtgcc aatatgtat aatgtcctg 
tacaaaaga tgtataaat gtttaccta gtcaaatat 
atcacgcgt taagac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY