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Gene information
General information
TC015823 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid43: 3685055..3694663
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015823
    iBeetle screen iB_02519
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva segment partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015823 RA
      MYESPNSLE DLCLDTICD NILIYVKLD HTNAGKRYK 
      FADPDLFLF KELSEKLLG KFLEKNILC DALMFIFSE 
      KNTKLSTVK LKNCKVTQN GLEILKQHK ILDLECVNL 
      KNVCIGNII DCLNEHSIR NMTNVNFAK CSFIDVQRH 
      SLMVKITQF KNLRSLNLA YTELNQHCL NMICEDLKL 
      LEKLDISGT GTGIKDLSP LRQLLNLTS LAISDLSQS 
      ANYVSTLTH LSTLRHLDV SVFNEKLDH LTTSNVVKL 
      LDDVTTLPH LVSLDVSGW RDFIPTETL LKYVETHPK 
      LEFLGIVLT DCTFDSLFS STNQWPNLT IAGLGNENQ 
      IKVTLKKYR ERSNYVQKA LYHLFQLTN LSPDARPDM 
      FNLVLPVMA AHSNKFGVQ MAATACLYN LTRGDLSKK 
      IHPKSLSRG VNLTLYAME CFPDEFQLQ KNSLLTLCS 
      DTILQEINF DRFRCAKLV LDALCTFED VNMNRMAVA 
      ICSILAAKV STEETSELG ARPVYMRKL LAMVQNRVE 
      SGVSDITLK FTLSALWNL TDESAATCT VFLEQGGAY 
      LFLKVLKTF KDDSAIETK VLGLLNNIA EVVKLRHSL 
      MMDPLMNEL FVLLKSENI DVSYFAAGI VAHLASDGA 
      EQWTVGSHT REEMLGELK NAVSQWKVP DSEMVAYRS 
      FRPFFSLLR IEMDYQVQL WAVWAIHHV CTKNPKRYC 
      VMLQDENGH LLLSDLINY TDSHPDIKS ISSQILETV 
      AGHV
    CDS sequence
    • >TC015823 RA
      atgtacgaa agtccgaat tctttggaa gatttgtgt 
      ttggacacg atttgtgac aatattctc atttacgtg 
      aaactcgat catacaaat gcgggaaaa cgttataaa 
      ttcgccgat cctgatttg ttcctcttc aaggaactg 
      tcagagaaa cttttaggg aaattcctc gagaagaat 
      atcctttgt gacgcctta atgtttata tttagtgag 
      aagaacacg aaactgagc actgttaaa ttaaaaaat 
      tgcaaagtg acacagaac ggacttgag atacttaaa 
      cagcacaag attttagat ttagaatgt gtcaatttg 
      aaaaatgtg tgtatagga aatattatt gattgtctc 
      aatgaacac tcaattaga aacatgacg aatgtgaat 
      ttcgctaaa tgctcgttt atcgacgtg cagaggcac 
      agtttgatg gtgaaaatt acacaattc aaaaatcta 
      cgctctctc aatttggcg tacactgaa ctcaaccaa 
      cactgctta aatatgatt tgtgaggac ctgaaacta 
      ctggaaaaa ctcgacatt tcgggaact ggcacgggg 
      atcaaggac cttagccct ctgaggcaa ctcctcaac 
      ttgacgagt ctcgcaatc agcgacttg tcgcagtca 
      gctaactac gtctcgacg ctcacccac ctgtccacc 
      ttacggcac ctcgacgtg agcgttttc aacgaaaaa 
      ctcgaccac ttgaccacc tccaacgtc gtcaaactc 
      ctcgacgac gtcacaacg ctcccccat ttagtctcg 
      ctcgatgta agcggctgg cgtgacttt atcccgact 
      gaaaccctc cttaaatac gttgaaact caccccaag 
      ttagaattt ctaggtatc gtccttacc gactgtacg 
      ttcgattcg ctgttcagt tctaccaac cagtggcca 
      aatttgaca atcgccggt ttgggaaac gaaaatcag 
      attaaagtc acgttaaag aagtataga gaacggtcg 
      aattatgta caaaaagcg ctctatcac ctgtttcaa 
      ttgacgaat ttatcgccg gatgcgcgt cccgacatg 
      tttaatttg gtacttccg gtcatggcg gcacattcg 
      aataagttt ggcgtgcaa atggccgcc actgcgtgt 
      ctgtataat ctaacacgg ggtgatttg agtaagaaa 
      atccatcca aagtcgttg agtcgaggg gtgaatctc 
      accctgtat gcgatggag tgtttcccc gatgagttt 
      cagttgcag aaaaattca cttttgacg ctttgcagc 
      gacaccata ttgcaggag ataaatttt gatcgtttt 
      cggtgcgca aagttagtt ttggacgcc ctttgtacg 
      ttcgaagac gtgaatatg aatcggatg gcggttgca 
      atttgtagt attttggcg gcgaaagtt agcaccgaa 
      gaaacgtcc gaattgggg gcacgcccc gtttacatg 
      cgtaaacta ctcgcaatg gttcagaat cgggtcgag 
      tcgggcgtt tccgacatc actcttaag ttcacactc 
      agcgccctc tggaacctt accgatgaa agtgccgcc 
      acctgtacg gtttttctg gagcaaggt ggcgcctac 
      ttgtttttg aaagttttg aaaacgttt aaagacgat 
      agtgcaatt gagacgaaa gttttaggt ttgttgaat 
      aatatagcc gaagttgtg aagctgagg catagtttg 
      atgatggat ccgttgatg aatgagttg tttgttttg 
      ttaaaatcg gaaaatatt gatgttagt tattttgcg 
      gccgggatt gtggcacat ttagcttca gatggggct 
      gagcaatgg acggtgggg agtcataca agggaggag 
      atgttgggg gagttgaaa aatgcggtc tcgcaatgg 
      aaagtgcca gatagtgaa atggtggcc tatcgcagc 
      tttaggccg tttttttca ctgttgagg atcgagatg 
      gactatcag gtgcagctg tgggcggtg tgggccata 
      catcacgtc tgtaccaaa aatccaaaa cggtactgc 
      gtcatgtta caagacgaa aacggccat ctcctcctc 
      agcgacttg atcaattac accgactcc catcccgat 
      attaaatca atctcgtct cagatcctc gaaacggtg 
      gctggccac gtttga
    mRNA sequence
    • >TC015823 RA
      gccgtcttt aaattgtga tagttttga aatgtcagg 
      tgtcacaac tttgacagt tcgaaatga aaatgagtt 
      tgacgataa attcttgat attccgtgt aacggttcg 
      attgcggca atgatcacc tcataaaga aaataaaag 
      cccaatcga agaaaaagt gaggttagg tgaaaatgt 
      acgaaagtc cgaattctt tggaagatt tgtgtttgg 
      acacgattt gtgacaata ttctcattt acgtgaaac 
      tcgatcata caaatgcgg gaaaacgtt ataaattcg 
      ccgatcctg atttgttcc tcttcaagg aactgtcag 
      agaaacttt tagggaaat tcctcgaga agaatatcc 
      tttgtgacg ccttaatgt ttatattta gtgagaaga 
      acacgaaac tgagcactg ttaaattaa aaaattgca 
      aagtgacac agaacggac ttgagatac ttaaacagc 
      acaagattt tagatttag aatgtgtca atttgaaaa 
      atgtgtgta taggaaata ttattgatt gtctcaatg 
      aacactcaa ttagaaaca tgacgaatg tgaatttcg 
      ctaaatgct cgtttatcg acgtgcaga ggcacagtt 
      tgatggtga aaattacac aattcaaaa atctacgct 
      ctctcaatt tggcgtaca ctgaactca accaacact 
      gcttaaata tgatttgtg aggacctga aactactgg 
      aaaaactcg acatttcgg gaactggca cggggatca 
      aggacctta gccctctga ggcaactcc tcaacttga 
      cgagtctcg caatcagcg acttgtcgc agtcagcta 
      actacgtct cgacgctca cccacctgt ccaccttac 
      ggcacctcg acgtgagcg ttttcaacg aaaaactcg 
      accacttga ccacctcca acgtcgtca aactcctcg 
      acgacgtca caacgctcc cccatttag tctcgctcg 
      atgtaagcg gctggcgtg actttatcc cgactgaaa 
      ccctcctta aatacgttg aaactcacc ccaagttag 
      aatttctag gtatcgtcc ttaccgact gtacgttcg 
      attcgctgt tcagttcta ccaaccagt ggccaaatt 
      tgacaatcg ccggtttgg gaaacgaaa atcagatta 
      aagtcacgt taaagaagt atagagaac ggtcgaatt 
      atgtacaaa aagcgctct atcacctgt ttcaattga 
      cgaatttat cgccggatg cgcgtcccg acatgttta 
      atttggtac ttccggtca tggcggcac attcgaata 
      agtttggcg tgcaaatgg ccgccactg cgtgtctgt 
      ataatctaa cacggggtg atttgagta agaaaatcc 
      atccaaagt cgttgagtc gaggggtga atctcaccc 
      tgtatgcga tggagtgtt tccccgatg agtttcagt 
      tgcagaaaa attcacttt tgacgcttt gcagcgaca 
      ccatattgc aggagataa attttgatc gttttcggt 
      gcgcaaagt tagttttgg acgcccttt gtacgttcg 
      aagacgtga atatgaatc ggatggcgg ttgcaattt 
      gtagtattt tggcggcga aagttagca ccgaagaaa 
      cgtccgaat tgggggcac gccccgttt acatgcgta 
      aactactcg caatggttc agaatcggg tcgagtcgg 
      gcgtttccg acatcactc ttaagttca cactcagcg 
      ccctctgga accttaccg atgaaagtg ccgccacct 
      gtacggttt ttctggagc aaggtggcg cctacttgt 
      ttttgaaag ttttgaaaa cgtttaaag acgatagtg 
      caattgaga cgaaagttt taggtttgt tgaataata 
      tagccgaag ttgtgaagc tgaggcata gtttgatga 
      tggatccgt tgatgaatg agttgtttg ttttgttaa 
      aatcggaaa atattgatg ttagttatt ttgcggccg 
      ggattgtgg cacatttag cttcagatg gggctgagc 
      aatggacgg tggggagtc atacaaggg aggagatgt 
      tgggggagt tgaaaaatg cggtctcgc aatggaaag 
      tgccagata gtgaaatgg tggcctatc gcagcttta 
      ggccgtttt tttcactgt tgaggatcg agatggact 
      atcaggtgc agctgtggg cggtgtggg ccatacatc 
      acgtctgta ccaaaaatc caaaacggt actgcgtca 
      tgttacaag acgaaaacg gccatctcc tcctcagcg 
      acttgatca attacaccg actcccatc ccgatatta 
      aatcaatct cgtctcaga tcctcgaaa cggtggctg 
      gccacgttt gaagatctc cagcagcat tattgtgat 
      ttacccata attctcagt tacttttta cttaggatt 
      gttaccact gtgagataa ttaagttgc gcccaactc 
      cccccgttg tatattttt attacgttg tcgattggt 
      tgtgtttta agacgtgcc aatatgtat aatgtcctg 
      tacaaaaga tgtataaat gtttaccta gtcaaatat 
      atcacgcgt taagac