General information | |||
TC015823 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007421.3: 8951912..8961520 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC015823 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_02519 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
remarks: muscle pattern potentially obscured by segmentation defect,
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%
larva segment partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
- >TC015823 RA
MYESPNSLE DLCLDTICD NILIYVKLD HTNAGKRYK FADPDLFLF KELSEKLLG KFLEKNILC DALMFIFSE KNTKLSTVK LKNCKVTQN GLEILKQHK ILDLECVNL KNVCIGNII DCLNEHSIR NMTNVNFAK CSFIDVQRH SLMVKITQF KNLRSLNLA YTELNQHCL NMICEDLKL LEKLDISGT GTGIKDLSP LRQLLNLTS LAISDLSQS ANYVSTLTH LSTLRHLDV SVFNEKLDH LTTSNVVKL LDDVTTLPH LVSLDVSGW RDFIPTETL LKYVETHPK LEFLGIVLT DCTFDSLFS STNQWPNLT IAGLGNENQ IKVTLKKYR ERSNYVQKA LYHLFQLTN LSPDARPDM FNLVLPVMA AHSNKFGVQ MAATACLYN LTRGDLSKK IHPKSLSRG VNLTLYAME CFPDEFQLQ KNSLLTLCS DTILQEINF DRFRCAKLV LDALCTFED VNMNRMAVA ICSILAAKV STEETSELG ARPVYMRKL LAMVQNRVE SGVSDITLK FTLSALWNL TDESAATCT VFLEQGGAY LFLKVLKTF KDDSAIETK VLGLLNNIA EVVKLRHSL MMDPLMNEL FVLLKSENI DVSYFAAGI VAHLASDGA EQWTVGSHT REEMLGELK NAVSQWKVP DSEMVAYRS FRPFFSLLR IEMDYQVQL WAVWAIHHV CTKNPKRYC VMLQDENGH LLLSDLINY TDSHPDIKS ISSQILETV AGHV
- >TC015823 RA
atgtacgaa agtccgaat tctttggaa gatttgtgt ttggacacg atttgtgac aatattctc atttacgtg aaactcgat catacaaat gcgggaaaa cgttataaa ttcgccgat cctgatttg ttcctcttc aaggaactg tcagagaaa cttttaggg aaattcctc gagaagaat atcctttgt gacgcctta atgtttata tttagtgag aagaacacg aaactgagc actgttaaa ttaaaaaat tgcaaagtg acacagaac ggacttgag atacttaaa cagcacaag attttagat ttagaatgt gtcaatttg aaaaatgtg tgtatagga aatattatt gattgtctc aatgaacac tcaattaga aacatgacg aatgtgaat ttcgctaaa tgctcgttt atcgacgtg cagaggcac agtttgatg gtgaaaatt acacaattc aaaaatcta cgctctctc aatttggcg tacactgaa ctcaaccaa cactgctta aatatgatt tgtgaggac ctgaaacta ctggaaaaa ctcgacatt tcgggaact ggcacgggg atcaaggac cttagccct ctgaggcaa ctcctcaac ttgacgagt ctcgcaatc agcgacttg tcgcagtca gctaactac gtctcgacg ctcacccac ctgtccacc ttacggcac ctcgacgtg agcgttttc aacgaaaaa ctcgaccac ttgaccacc tccaacgtc gtcaaactc ctcgacgac gtcacaacg ctcccccat ttagtctcg ctcgatgta agcggctgg cgtgacttt atcccgact gaaaccctc cttaaatac gttgaaact caccccaag ttagaattt ctaggtatc gtccttacc gactgtacg ttcgattcg ctgttcagt tctaccaac cagtggcca aatttgaca atcgccggt ttgggaaac gaaaatcag attaaagtc acgttaaag aagtataga gaacggtcg aattatgta caaaaagcg ctctatcac ctgtttcaa ttgacgaat ttatcgccg gatgcgcgt cccgacatg tttaatttg gtacttccg gtcatggcg gcacattcg aataagttt ggcgtgcaa atggccgcc actgcgtgt ctgtataat ctaacacgg ggtgatttg agtaagaaa atccatcca aagtcgttg agtcgaggg gtgaatctc accctgtat gcgatggag tgtttcccc gatgagttt cagttgcag aaaaattca cttttgacg ctttgcagc gacaccata ttgcaggag ataaatttt gatcgtttt cggtgcgca aagttagtt ttggacgcc ctttgtacg ttcgaagac gtgaatatg aatcggatg gcggttgca atttgtagt attttggcg gcgaaagtt agcaccgaa gaaacgtcc gaattgggg gcacgcccc gtttacatg cgtaaacta ctcgcaatg gttcagaat cgggtcgag tcgggcgtt tccgacatc actcttaag ttcacactc agcgccctc tggaacctt accgatgaa agtgccgcc acctgtacg gtttttctg gagcaaggt ggcgcctac ttgtttttg aaagttttg aaaacgttt aaagacgat agtgcaatt gagacgaaa gttttaggt ttgttgaat aatatagcc gaagttgtg aagctgagg catagtttg atgatggat ccgttgatg aatgagttg tttgttttg ttaaaatcg gaaaatatt gatgttagt tattttgcg gccgggatt gtggcacat ttagcttca gatggggct gagcaatgg acggtgggg agtcataca agggaggag atgttgggg gagttgaaa aatgcggtc tcgcaatgg aaagtgcca gatagtgaa atggtggcc tatcgcagc tttaggccg tttttttca ctgttgagg atcgagatg gactatcag gtgcagctg tgggcggtg tgggccata catcacgtc tgtaccaaa aatccaaaa cggtactgc gtcatgtta caagacgaa aacggccat ctcctcctc agcgacttg atcaattac accgactcc catcccgat attaaatca atctcgtct cagatcctc gaaacggtg gctggccac gtttga
- >TC015823 RA
gccgtcttt aaattgtga tagttttga aatgtcagg tgtcacaac tttgacagt tcgaaatga aaatgagtt tgacgataa attcttgat attccgtgt aacggttcg attgcggca atgatcacc tcataaaga aaataaaag cccaatcga agaaaaagt gaggttagg tgaaaatgt acgaaagtc cgaattctt tggaagatt tgtgtttgg acacgattt gtgacaata ttctcattt acgtgaaac tcgatcata caaatgcgg gaaaacgtt ataaattcg ccgatcctg atttgttcc tcttcaagg aactgtcag agaaacttt tagggaaat tcctcgaga agaatatcc tttgtgacg ccttaatgt ttatattta gtgagaaga acacgaaac tgagcactg ttaaattaa aaaattgca aagtgacac agaacggac ttgagatac ttaaacagc acaagattt tagatttag aatgtgtca atttgaaaa atgtgtgta taggaaata ttattgatt gtctcaatg aacactcaa ttagaaaca tgacgaatg tgaatttcg ctaaatgct cgtttatcg acgtgcaga ggcacagtt tgatggtga aaattacac aattcaaaa atctacgct ctctcaatt tggcgtaca ctgaactca accaacact gcttaaata tgatttgtg aggacctga aactactgg aaaaactcg acatttcgg gaactggca cggggatca aggacctta gccctctga ggcaactcc tcaacttga cgagtctcg caatcagcg acttgtcgc agtcagcta actacgtct cgacgctca cccacctgt ccaccttac ggcacctcg acgtgagcg ttttcaacg aaaaactcg accacttga ccacctcca acgtcgtca aactcctcg acgacgtca caacgctcc cccatttag tctcgctcg atgtaagcg gctggcgtg actttatcc cgactgaaa ccctcctta aatacgttg aaactcacc ccaagttag aatttctag gtatcgtcc ttaccgact gtacgttcg attcgctgt tcagttcta ccaaccagt ggccaaatt tgacaatcg ccggtttgg gaaacgaaa atcagatta aagtcacgt taaagaagt atagagaac ggtcgaatt atgtacaaa aagcgctct atcacctgt ttcaattga cgaatttat cgccggatg cgcgtcccg acatgttta atttggtac ttccggtca tggcggcac attcgaata agtttggcg tgcaaatgg ccgccactg cgtgtctgt ataatctaa cacggggtg atttgagta agaaaatcc atccaaagt cgttgagtc gaggggtga atctcaccc tgtatgcga tggagtgtt tccccgatg agtttcagt tgcagaaaa attcacttt tgacgcttt gcagcgaca ccatattgc aggagataa attttgatc gttttcggt gcgcaaagt tagttttgg acgcccttt gtacgttcg aagacgtga atatgaatc ggatggcgg ttgcaattt gtagtattt tggcggcga aagttagca ccgaagaaa cgtccgaat tgggggcac gccccgttt acatgcgta aactactcg caatggttc agaatcggg tcgagtcgg gcgtttccg acatcactc ttaagttca cactcagcg ccctctgga accttaccg atgaaagtg ccgccacct gtacggttt ttctggagc aaggtggcg cctacttgt ttttgaaag ttttgaaaa cgtttaaag acgatagtg caattgaga cgaaagttt taggtttgt tgaataata tagccgaag ttgtgaagc tgaggcata gtttgatga tggatccgt tgatgaatg agttgtttg ttttgttaa aatcggaaa atattgatg ttagttatt ttgcggccg ggattgtgg cacatttag cttcagatg gggctgagc aatggacgg tggggagtc atacaaggg aggagatgt tgggggagt tgaaaaatg cggtctcgc aatggaaag tgccagata gtgaaatgg tggcctatc gcagcttta ggccgtttt tttcactgt tgaggatcg agatggact atcaggtgc agctgtggg cggtgtggg ccatacatc acgtctgta ccaaaaatc caaaacggt actgcgtca tgttacaag acgaaaacg gccatctcc tcctcagcg acttgatca attacaccg actcccatc ccgatatta aatcaatct cgtctcaga tcctcgaaa cggtggctg gccacgttt gaagatctc cagcagcat tattgtgat ttacccata attctcagt tacttttta cttaggatt gttaccact gtgagataa ttaagttgc gcccaactc cccccgttg tatattttt attacgttg tcgattggt tgtgtttta agacgtgcc aatatgtat aatgtcctg tacaaaaga tgtataaat gtttaccta gtcaaatat atcacgcgt taagac
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
