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Gene information
General information
TC015810 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 8822203..8827520
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC015810
      iBeetle screen iB_02515

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
      Days post injection


      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): less than 30%

      embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

      embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

      larval musculature partially randomnot present - Penetrance (percentage of animals/eggs on slide): less than 30%

      larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
      cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%

      head shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      antenna irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      Comment: antenna can be malformed, shortened or bent. flagellum is very often missing. mandibles can also be missing in rare cases.
      antenna shortened - Penetrance (percentage of animals/eggs on slide): less than 30%

      Comment:
      antenna not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      head partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

      thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
      abdomen partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

      abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 80.0% (includes death as larva, prepupa, pupa, adult)

      pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
      Days post injection


      eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
      Days post injection


      Fertility
      fertility after larval injection decreased (no living offspring) -
      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC015810 RA
        MSNSRNDCR IYVGNLPPD IRTKDIQDL 
        FYKFGKVTF VDLKNRRGP PFAFVEFED 
        PRDADDAVH ARDGYDYDG YRLRVEFPR 
        GGGHGSFRG GRGGGDRGR SSRGPPARR 
        SQFRVLVTG LPPSGSWQD LKDHMREAG 
        DVCFADAFK DGSGVVEFL RYEDMKYAI 
        KKLDDSRFR SHEGEVSYI RVKEDRGGS 
        DRRSGGRSR SRSYSPRRR GSPTYSPVK 
        RSYSRSRSR S
      CDS sequence
      • >TC015810 RA
        atgtccaac agccgcaac gactgccga atctacgtc 
        gggaatctg cccccggac atccgcacc aaagacatc 
        caagacctg ttttacaaa ttcggcaaa gttacattc 
        gtcgacttg aaaaaccga cggggacct cctttcgcc 
        ttcgtcgag ttcgaggac cccagggac gcggacgac 
        gccgtacat gcacgggac ggttacgac tacgacggc 
        taccgcctc cgggtggaa ttcccgagg ggcggcggc 
        catggcagc ttcaggggt ggccgcggc ggcggcgac 
        aggggacgc agctccagg gggccacca gctcgcagg 
        tcgcaattt agagtgctt gtgacaggg ttgccaccg 
        tcgggctcg tggcaggac ttgaaggac cacatgcgg 
        gaggctgga gacgtgtgc ttcgcggac gcgttcaag 
        gacggttca ggcgttgtg gagtttctc cgatacgaa 
        gacatgaaa tatgctata aagaaattg gacgattcg 
        aggtttagg tcacatgag ggggaggtt tcgtacata 
        agggtgaag gaagatcgt ggggggagt gatagacgg 
        tcaggggga aggtcacgc agtcgttca tattcgccc 
        cgtcggagg ggttcccca acatacagt ccggtcaaa 
        aggagttac tccagaagc cgttctcgc tcctaa
      mRNA sequence
      • >TC015810 RA
        gccatcaca aacgccctc acgtcgcca tctttcctt 
        cttgtttat cgccgattt cgtccactt tccaacacg 
        aaacgatgt ccaacagcc gcaacgact gccgaatct 
        acgtcggga atctgcccc cggacatcc gcaccaaag 
        acatccaag acctgtttt acaaattcg gcaaagtta 
        cattcgtcg acttgaaaa accgacggg gacctcctt 
        tcgccttcg tcgagttcg aggacccca gggacgcgg 
        acgacgccg tacatgcac gggacggtt acgactacg 
        acggctacc gcctccggg tggaattcc cgaggggcg 
        gcggccatg gcagcttca ggggtggcc gcggcggcg 
        gcgacaggg gacgcagct ccagggggc caccagctc 
        gcaggtcgc aatttagag tgcttgtga cagggttgc 
        caccgtcgg gctcgtggc aggacttga aggaccaca 
        tgcgggagg ctggagacg tgtgcttcg cggacgcgt 
        tcaaggacg gttcaggcg ttgtggagt ttctccgat 
        acgaagaca tgaaatatg ctataaaga aattggacg 
        attcgaggt ttaggtcac atgaggggg aggtttcgt 
        acataaggg tgaaggaag atcgtgggg ggagtgata 
        gacggtcag ggggaaggt cacgcagtc gttcatatt 
        cgccccgtc ggaggggtt ccccaacat acagtccgg 
        tcaaaagga gttactcca gaagccgtt ctcgctcct 
        aatttaatc tactctacg gactggcct ttttgtata 
        atgtacgta tcaaaaaaa ccggggatt tttttaatt 
        tgattgatt taaaaaaaa tgcaagaga aaaaaagcg 
        gttggtaac cctggaatt tgtatttgt aaatgtggg 
        tattttaat tcttctgac attcctgga agaatcgtt 
        tttttctct cctccattc ttgtaattt tttagactt 
        aaattttat aaaaatttt caattaggt tagagtacg 
        gataaggca ctaatatgt attgtttct tttttctgt 
        taccgtaca atttgctga gtcaatcag cgttggact 
        ggtttttgg aaaaaataa taaagtcga tagtaaaat 
        aatagacaa taaaatgtt atctttgac acacccgga 
        tgcaagagg agtggatga atgttgtta ggagggagt 
        ctcaaagat cgtggagga ggatattta aacttttaa 
        cacactaag cattgcatt tttcggagt gttgagagg 
        acaccacac aattaaatg gctcggtaa ataagttat 
        tcaattttt ctaacaatg aaacacaat catttcaaa 
        aaattaact gacacgttt ctcattgaa aaaacagtg 
        tttaaacga gatgttttg caatgcgat aggtctcac 
        aaacacggg attttgatc tccacttca ccggaagga 
        aaggacaaa ctcctagga ttaggattt tttaaataa 
        attgtagta attttc
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY