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Gene information
General information
TC015781 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 7992013..7997322
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015781
    iBeetle screen iB_02512

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    egg chamber mostly decreased - Number of animals showing the phenotype: 3

    egg chamber structure irregular - Number of animals showing the phenotype: 3

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection



    pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 4 (19 dpi)
    Days post injection


    Pupal morphology
    antenna shortened - Number of animals showing the phenotype: 2

    head pigmentation/tanning spotted - Number of animals showing the phenotype: 3
    wings shortened - Number of animals showing the phenotype: 3

    legs potentially shape irregular - Number of animals showing the phenotype: 3
    Comment: difficult to analyze because animals were dead for a while and dried out
    legs potentially shortened - Number of animals showing the phenotype: 3
    wings partially not present - Number of animals showing the phenotype: 2
    Comment: potentially due to cannibalism and dryout
    thorax pigmentation/tanning spotted - Number of animals showing the phenotype: 3
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015781 RA
      MKNLELLEL VHKGVPYPF TNVTLVSQT PQLIYYIRA 
      NSLHSHDLT CGETIQLGP PIFADASHL KSLIIENKT 
      CVVVNNDLF IIALPNKFD KITFDKKII EISWNPTEE 
      LVAVVFDDG EISTFCIDY ENAEAFPQG KSSTDAGIP 
      DTVYVGWGS KDTQFRGSE GKIKDQTPV ETKPVFDQK 
      PRISWRGNG EMFVVNYWK DQKRQFIVF ETPCKALYR 
      SEECPGLQP QVAWRPVGN MIAGLSVTN RQKIVIFEK 
      NGQRRFDFD LTFDVMIKN LKWSPCAQI LAIHTVTPT 
      GQTIHLLTS SNYKWYEKQ VLEFPAENA LLDFDWLDT 
      NQLQVVTQS DVIKYTFRN VVHHNSAAI CGVIDGKHL 
      NLTDFNNAV IPPISYARR FTNDKQINF VTFRHDLAM 
      IIDSENDLK IFNVAEPDA LLVTINLKK IVDLPQFAL 
      SCHHFLLSS ESVYFAVTS DESVFYSLD WKNPQANFL 
      TAITDNFSH LLQISGDYD NISGLKSLG NNLFINNND 
      SILCQIQTL GDQTYVCNL TSNNHFYLN EAKISDCAN 
      SFTLFDSYL LYTTKQSEL FCLRLGQEG QNFRRNVEQ 
      GATIVCAVP NSPQIVLQL PRGNLETIS CRLISIDIL 
      DKLLNEQKW AEAVRFIRL EKLNANLLF DLNPERFLR 
      QIAHFVQGV HTINELTAI CLEFEEGNV LTSIYKNWG 
      KTTDFPQKI NTIFASLFK YFDSVDYSV YITTIVAVN 
      LNFFKLRDA LIYLQDLYR RTNLKEKLL NAVNTLKIY 
      GCDHEKLYT ECLLLYDLE LAGFIASCC QLDPRVYEP 
      HLKQLSGLN EVEMRYEIN LFAKKPKTA IIYLLRCPK 
      AQTSDVLAF IKTHNVSRQ AFENCPPKN RFYQSVSHA 
      FAGDLSAKG CHTEAGVVL KRAGLPEEA LAEFQLGLN 
      WRQVLNLLE ELNVDKVEK IKIVNDLAT RLVQSNVRQ 
      AAILFEFYA DNYEMAVKV LIEGFFFEE AIHIAMKHK 
      RGDIIVSDV IPMLMKHKI YLEEKLQNL NESYNKYKQ 
      RLAQVRQQK FSRFNNDLD DCDERDDLF SDAGSTISK 
      SSRSSRSRS STASSRNRR KEEKKKQDL REGGIYEDI 
      ALIRALHST IKEFYNKGE EVRNCCVLL LQESDVSYD 
      EIKRIHDFY WKFDAEIQN GVAEIWPPH FYKNYQTLD 
      VRELADLEN FEHLDVEFR TPPGGRTNW KLNLF
    CDS sequence
    • >TC015781 RA
      atgaaaaac ttagaattg ctcgaatta gtgcacaaa 
      ggggtcccc taccccttc accaacgtg actctagtg 
      tcccaaacg ccccaacta atttattac atccgtgcc 
      aattcttta cacagtcat gacctaaca tgtggcgaa 
      accatccaa ctgggcccc cctatcttc gcagacgcc 
      tctcatcta aaatcctta atcattgag aacaaaact 
      tgtgttgtt gttaacaat gatttgttt ataatcgcc 
      cttccaaac aaatttgac aaaatcacc ttcgacaaa 
      aaaatcatc gaaatcagc tggaacccc acggaagaa 
      ctcgtcgcg gtggtgttc gacgatgga gaaatatcg 
      actttttgt attgactac gaaaacgcc gaagctttc 
      ccccaaggg aaaagcagc accgatgcg ggaatcccc 
      gacacagtt tacgtgggt tgggggtcc aaggacacg 
      caatttcgg ggctccgaa ggcaaaatc aaggatcaa 
      acaccggtc gaaaccaag ccagttttt gaccaaaaa 
      ccccgaatt tcatggcga ggaaacggg gaaatgttc 
      gttgtgaat tattggaag gaccagaaa agacaattc 
      attgtgttc gagacaccc tgtaaagca ttgtaccgc 
      tcggaggag tgccccggg ttacagccc caggtggcc 
      tggcggccc gtcgggaac atgatagct gggctgtcg 
      gtcacaaac cggcaaaaa atcgtaatt tttgaaaaa 
      aatggccag aggaggttt gatttcgat ctgacgttt 
      gacgttatg atcaaaaac ttgaagtgg agtccctgt 
      gcccaaatt cttgccatc cacactgtc acacccacc 
      ggtcaaacc atccattta ttgacctca agtaactac 
      aagtggtac gagaaacaa gtgcttgaa tttcccgcc 
      gaaaacgcg ctcctcgat ttcgattgg ctggacacc 
      aaccagtta caagtcgtt acgcaaagt gacgtaatt 
      aagtacacg tttcgtaac gttgttcat cacaattcg 
      gccgcgatt tgtggcgta atcgacggc aaacacttg 
      aatttgact gattttaac aacgcggtt attcccccg 
      atttcgtac gcacgacga ttcacgaac gacaaacaa 
      atcaacttc gtgacgttc cgtcacgac ttagctatg 
      atcattgac tcggaaaat gatttaaaa attttcaat 
      gttgctgaa cctgacgcg cttttagtt acaataaat 
      ttgaaaaaa atcgtcgat ttgccacaa tttgcgttg 
      tcttgtcat catttttta ttatccagt gaaagcgtt 
      tatttcgct gtaacgagt gacgaatcg gttttctat 
      tcccttgat tggaaaaat ccccaagca aatttccta 
      acggcaatc accgataat ttcagccat ttattgcaa 
      ataagtggg gattatgac aacatttcc ggcctgaaa 
      tcactcgga aataactta ttcattaat aataacgac 
      tcaattctt tgccaaatt caaaccctc ggtgatcaa 
      acctacgtt tgcaaccta acgtcaaat aatcacttc 
      tacctcaat gaggcaaaa atcagcgat tgtgcgaat 
      tctttcaca ctgttcgac tcgtattta ctttacacc 
      accaaacaa agtgagttg ttttgcctc agattgggc 
      caagagggc caaaatttc cgtcgaaac gtggaacag 
      ggggccact atcgtttgt gcggtcccc aattcgccc 
      caaattgtc ttgcagttg ccccgaggc aacttggag 
      acaatttcg tgtcgtttg atctcgatc gacatcctc 
      gataaacta ctgaacgaa cagaaatgg gccgaagct 
      gtgcgtttc atccgtttg gaaaaatta aacgcaaat 
      ttgttattt gatttgaat ccggagcgg tttttgcgc 
      caaatcgcc cactttgtg cagggggtt cacacgatt 
      aatgagcta accgccatt tgcttggag ttcgaggag 
      gggaacgtc ctgacctcg atttacaaa aattggggg 
      aaaaccacc gatttcccc cagaaaatt aacacgatt 
      tttgcgagt ttgtttaaa tatttcgac agtgtcgac 
      tattccgtt tacataacc acgatagtg gcggttaat 
      ttgaatttt ttcaaactg agggacgcc ttgatttat 
      ctacaagac ctgtacagg cgtaccaac ctaaaggaa 
      aagctgtta aatgccgta aatacgtta aaaatctac 
      gggtgtgac cacgaaaaa ctatacact gagtgttta 
      ttattgtac gatttggag ttggctggt tttatcgcg 
      agttgttgc caactagat ccgcgagtt tacgaacca 
      catttgaag cagttgagt ggtttaaat gaggttgaa 
      atgcgctac gagattaac ttatttgcg aaaaaaccc 
      aaaactgcg attatttat ctgttacgg tgccccaaa 
      gcacaaaca agtgacgtt ttagcgttt attaaaacc 
      cacaatgtg agcaggcaa gctttcgag aattgtccg 
      ccaaaaaat aggttttac caaagcgta agtcacgct 
      tttgcgggc gatttgagt gcgaaaggg tgtcacacc 
      gaagctggg gttgttcta aaaagggcg ggtttacca 
      gaggaggct ttggccgag tttcaattg gggctgaat 
      tggcgccaa gttttgaat ctcctggaa gagttaaac 
      gtcgataaa gtggaaaaa atcaagatc gtgaacgat 
      ttggccacg cgtttggtg cagagtaat gtccgccaa 
      gccgccatt ttgttcgag ttttatgca gataactac 
      gaaatggcg gttaaggta cttattgag ggttttttc 
      ttcgaggag gcgattcat atcgctatg aaacacaaa 
      cggggggat attatagtc tcggacgtg attccaatg 
      ttaatgaag cacaaaata tacttggaa gagaagttg 
      caaaatttg aacgaaagc tacaataaa tacaaacag 
      aggttggcc caagtcagg caacaaaag tttagtaga 
      tttaacaac gacttggac gattgtgac gagcgggat 
      gaccttttt tccgacgct gggagtaca atttccaaa 
      tcttcacgt tcatcaagg tcgcgctcg tcaaccgcg 
      tcatcgcga aatcgccga aaagaagag aaaaagaag 
      caagatctt cgcgaaggc gggatttac gaagatata 
      gcgctcatt cgcgccctc cattccacc attaaagag 
      ttttacaat aaaggtgaa gaagtccgg aattgttgt 
      gttttatta ttgcaagaa agcgacgtt tcgtacgat 
      gaaattaag cgaattcat gatttttac tggaagttt 
      gatgccgaa attcaaaat ggcgtcgcg gaaatatgg 
      ccgccacat ttttacaag aattatcaa acgttggat 
      gtgcgagaa ttggccgat ttggagaat tttgaacat 
      ttagatgtt gagtttagg acacctccc gggggccga 
      accaactgg aaattgaac cttttttaa
    mRNA sequence
    • >TC015781 RA
      ttttccaaa ccatgaaaa acttagaat tgctcgaat 
      tagtgcaca aaggggtcc cctacccct tcaccaacg 
      tgactctag tgtcccaaa cgccccaac taatttatt 
      acatccgtg ccaattctt tacacagtc atgacctaa 
      catgtggcg aaaccatcc aactgggcc cccctatct 
      tcgcagacg cctctcatc taaaatcct taatcattg 
      agaacaaaa cttgtgttg ttgttaaca atgatttgt 
      ttataatcg cccttccaa acaaatttg acaaaatca 
      ccttcgaca aaaaaatca tcgaaatca gctggaacc 
      ccacggaag aactcgtcg cggtggtgt tcgacgatg 
      gagaaatat cgacttttt gtattgact acgaaaacg 
      ccgaagctt tcccccaag ggaaaagca gcaccgatg 
      cgggaatcc ccgacacag tttacgtgg gttgggggt 
      ccaaggaca cgcaatttc ggggctccg aaggcaaaa 
      tcaaggatc aaacaccgg tcgaaacca agccagttt 
      ttgaccaaa aaccccgaa tttcatggc gaggaaacg 
      gggaaatgt tcgttgtga attattgga aggaccaga 
      aaagacaat tcattgtgt tcgagacac cctgtaaag 
      cattgtacc gctcggagg agtgccccg ggttacagc 
      cccaggtgg cctggcggc ccgtcggga acatgatag 
      ctgggctgt cggtcacaa accggcaaa aaatcgtaa 
      tttttgaaa aaaatggcc agaggaggt ttgatttcg 
      atctgacgt ttgacgtta tgatcaaaa acttgaagt 
      ggagtccct gtgcccaaa ttcttgcca tccacactg 
      tcacaccca ccggtcaaa ccatccatt tattgacct 
      caagtaact acaagtggt acgagaaac aagtgcttg 
      aatttcccg ccgaaaacg cgctcctcg atttcgatt 
      ggctggaca ccaaccagt tacaagtcg ttacgcaaa 
      gtgacgtaa ttaagtaca cgtttcgta acgttgttc 
      atcacaatt cggccgcga tttgtggcg taatcgacg 
      gcaaacact tgaatttga ctgatttta acaacgcgg 
      ttattcccc cgatttcgt acgcacgac gattcacga 
      acgacaaac aaatcaact tcgtgacgt tccgtcacg 
      acttagcta tgatcattg actcggaaa atgatttaa 
      aaattttca atgttgctg aacctgacg cgcttttag 
      ttacaataa atttgaaaa aaatcgtcg atttgccac 
      aatttgcgt tgtcttgtc atcattttt tattatcca 
      gtgaaagcg tttatttcg ctgtaacga gtgacgaat 
      cggttttct attcccttg attggaaaa atccccaag 
      caaatttcc taacggcaa tcaccgata atttcagcc 
      atttattgc aaataagtg gggattatg acaacattt 
      ccggcctga aatcactcg gaaataact tattcatta 
      ataataacg actcaattc tttgccaaa ttcaaaccc 
      tcggtgatc aaacctacg tttgcaacc taacgtcaa 
      ataatcact tctacctca atgaggcaa aaatcagcg 
      attgtgcga attctttca cactgttcg actcgtatt 
      tactttaca ccaccaaac aaagtgagt tgttttgcc 
      tcagattgg gccaagagg gccaaaatt tccgtcgaa 
      acgtggaac agggggcca ctatcgttt gtgcggtcc 
      ccaattcgc cccaaattg tcttgcagt tgccccgag 
      gcaacttgg agacaattt cgtgtcgtt tgatctcga 
      tcgacatcc tcgataaac tactgaacg aacagaaat 
      gggccgaag ctgtgcgtt tcatccgtt tggaaaaat 
      taaacgcaa atttgttat ttgatttga atccggagc 
      ggtttttgc gccaaatcg cccactttg tgcaggggg 
      ttcacacga ttaatgagc taaccgcca tttgcttgg 
      agttcgagg aggggaacg tcctgacct cgatttaca 
      aaaattggg ggaaaacca ccgatttcc cccagaaaa 
      ttaacacga tttttgcga gtttgttta aatatttcg 
      acagtgtcg actattccg tttacataa ccacgatag 
      tggcggtta atttgaatt ttttcaaac tgagggacg 
      ccttgattt atctacaag acctgtaca ggcgtacca 
      acctaaagg aaaagctgt taaatgccg taaatacgt 
      taaaaatct acgggtgtg accacgaaa aactataca 
      ctgagtgtt tattattgt acgatttgg agttggctg 
      gttttatcg cgagttgtt gccaactag atccgcgag 
      tttacgaac cacatttga agcagttga gtggtttaa 
      atgaggttg aaatgcgct acgagatta acttatttg 
      cgaaaaaac ccaaaactg cgattattt atctgttac 
      ggtgcccca aagcacaaa caagtgacg ttttagcgt 
      ttattaaaa cccacaatg tgagcaggc aagctttcg 
      agaattgtc cgccaaaaa ataggtttt accaaagcg 
      taagtcacg cttttgcgg gcgatttga gtgcgaaag 
      ggtgtcaca ccgaagctg gggttgttc taaaaaggg 
      cgggtttac cagaggagg ctttggccg agtttcaat 
      tggggctga attggcgcc aagttttga atctcctgg 
      aagagttaa acgtcgata aagtggaaa aaatcaaga 
      tcgtgaacg atttggcca cgcgtttgg tgcagagta 
      atgtccgcc aagccgcca ttttgttcg agttttatg 
      cagataact acgaaatgg cggttaagg tacttattg 
      agggttttt tcttcgagg aggcgattc atatcgcta 
      tgaaacaca aacgggggg atattatag tctcggacg 
      tgattccaa tgttaatga agcacaaaa tatacttgg 
      aagagaagt tgcaaaatt tgaacgaaa gctacaata 
      aatacaaac agaggttgg cccaagtca ggcaacaaa 
      agtttagta gatttaaca acgacttgg acgattgtg 
      acgagcggg atgaccttt tttccgacg ctgggagta 
      caatttcca aatcttcac gttcatcaa ggtcgcgct 
      cgtcaaccg cgtcatcgc gaaatcgcc gaaaagaag 
      agaaaaaga agcaagatc ttcgcgaag gcgggattt 
      acgaagata tagcgctca ttcgcgccc tccattcca 
      ccattaaag agttttaca ataaaggtg aagaagtcc 
      ggaattgtt gtgttttat tattgcaag aaagcgacg 
      tttcgtacg atgaaatta agcgaattc atgattttt 
      actggaagt ttgatgccg aaattcaaa atggcgtcg 
      cggaaatat ggccgccac atttttaca agaattatc 
      aaacgttgg atgtgcgag aattggccg atttggaga 
      attttgaac atttagatg ttgagttta ggacacctc 
      ccgggggcc gaaccaact ggaaattga acctttttt 
      aatattgta ctcgtagac aataaatta tttttattt 
      tg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY