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Gene information
General information
TC015727 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid43: 514000..518301
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015727
    iBeetle screen iB_02509
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 5 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality late (L7) - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015727 RA
      MTDERLHID WGNDKLHRT QKQVERNPY DLESWSILL 
      REVQTKHIS EVRPLYEHL IDIFPSASR YWRIYIEHE 
      MKSRNFERV EKLFQRCLM KILNIELWK LYLGYVKET 
      KASLPTYKE KMAQAYDFA LDKIGMDIH SYSIWNEYV 
      NFLKGVEAV GSYAENQKI SAVRKVYQR GINNPMTGM 
      ETFWKDYIA FEQAINPII AEKMSIERS RDYMNARRV 
      AKELEVQIR GINRNAPSV PPNGSPEER KQVELWQKY 
      IAWEKSNPL RTEDTALLT KRVVFALEQ CLLCLGHHP 
      DIWYQAAQF LEYSCKILA EKGDVNASK LFSEEAANM 
      FERATSSLL NKNMLLYFA YADYEEGRL KYEKVHQIY 
      QKYLEIQDI DPTLAYIQY MKFARRAEG IKSARAVFK 
      RAREDNRSK YHIFVCAAL MEYYCSKDK NIAFRIFEL 
      GLKKFGDIP EYITCYIDY LSHLNEDNN TRVLFERVL 
      SSGSLEPEK SVDIWNRFL EFECNIGDL QSIVKVEKR 
      RSEVLSKIK EFEGKETAQ LVDRYKFLD LYPCTATEL 
      KSIGYTEVI NITGAGRNH ILQSIINLD GDAPLPLPD 
      YSQMIPYKP KSNPLPGEH PVPGGAFPH PPVAAYLCT 
      LLPPPACFR GPFVNVDLL MDIFNRIQL PDSPPQPSE 
      NGGDVRLFD LAKSVHWIV EEGGGVGIK RKKRLGLAM 
      DGSDDEDLP LPPTNDIYR QRQQKRVK
    CDS sequence
    • >TC015727 RA
      atgaccgac gagaggctc cacattgac tggggtaac 
      gataagttg caccgcacc cagaagcag gtcgagcgc 
      aacccctac gacctcgaa tcatggtcg attttgctg 
      cgcgaggtg caaaccaag cacatttcg gaagtgcgc 
      cccctttac gagcacctc atcgacata ttccccagc 
      gcgtcgcgc tactggcgc atctacatc gagcacgag 
      atgaagtcc cggaatttc gaaagagtg gaaaagttg 
      tttcaaagg tgtttgatg aagatctta aatattgag 
      ttgtggaaa ctttatctc ggatatgtt aaagaaacc 
      aaggcttcg ctcccgacc tataaggaa aaaatggca 
      caagcctac gactttgcc ttggacaaa atcggaatg 
      gacatccat tcgtactcg atttggaac gaatacgtg 
      aatttttta aagggggtg gaggccgtg ggctcgtat 
      gcggagaac cagaaaatt tcagcggtg cgtaaggtc 
      taccaaagg ggcattaat aaccccatg acggggatg 
      gagaccttc tggaaggac tacatagcc ttcgaacaa 
      gccattaac cccattatt gcggaaaaa atgagcatc 
      gagaggagt cgcgattac atgaacgcg cgcagagtc 
      gccaaagaa ctcgaagta cagattcgg gggattaac 
      aggaacgcc cccagtgtg ccccccaac ggaagccca 
      gaagagagg aaacaagtc gagctgtgg cagaaatat 
      atcgcgtgg gagaagagc aatccgctt cgcaccgaa 
      gacacggcc ctcttgacc aaacgagtg gtcttcgcc 
      cttgagcaa tgcctactt tgtttggga caccaccct 
      gatatttgg taccaagcg gcacagttt ctcgaatac 
      agttgtaaa attctagcc gagaaaggg gacgtcaac 
      gcttcgaaa ttatttagc gaagaggcg gcaaatatg 
      ttcgagagg gcgacgagc tcgctcttg aacaaaaac 
      atgctactt tacttcgcc tacgctgat tacgaggaa 
      gggcgtttg aaatacgaa aaagtccac cagatttac 
      caaaagtac cttgaaatt caagatatc gacccgaca 
      cttgcgtac atccagtat atgaaattc gccagacgg 
      gccgaaggg atcaagtcg gctcgggcc gttttcaaa 
      agagcgcgc gaagacaat cgctccaag taccacatt 
      ttcgtctgc gccgccttg atggagtac tattgcagt 
      aaagacaaa aacatcgcg tttcgaatt ttcgaactg 
      ggtttgaaa aaattcggg gatatcccc gaatatatc 
      acgtgttac atcgactat ttgtcacac ttgaacgaa 
      gacaacaac acgcgagtg ctattcgag cgcgtcctg 
      tcatctgga agtctagaa ccggaaaaa tccgttgac 
      atttggaac cgttttctg gaattcgag tgcaatatc 
      ggggattta cagagtatt gtcaaagtt gagaaaaga 
      cgatcggaa gtcttgtca aaaattaaa gaatttgaa 
      ggcaaagaa acggcacaa ttggtcgac agatacaaa 
      tttttagat ttgtatccc tgcacggcc acggaactc 
      aaaagtata gggtataca gaagtgatc aacataacg 
      ggggcgggg cgcaaccac attttacag tcgataatc 
      aacctcgac ggcgacgcc cccttgccc ctgcccgac 
      tactcacaa atgatcccc tacaagccc aagtccaac 
      ccccttccg ggcgagcat ccggtcccc ggcggtgcg 
      ttcccccac cctccggtg gcggcgtac ctgtgcacg 
      cttctgccg cccccggca tgtttccgg gggcctttc 
      gtcaacgtg gacctcctc atggacatt ttcaaccgg 
      atccagtta ccggattcg cccccgcag ccgtcggaa 
      aatgggggc gatgtgagg ctttttgat ttggcaaaa 
      tccgttcat tggatcgtc gaggaaggg ggcggagtc 
      gggattaag aggaagaag agactggga ttggctatg 
      gatgggtca gatgatgag gatttgccg ctgccgccg 
      accaatgat atttacagg cagcggcaa cagaagcgt 
      gtgaaatga
    mRNA sequence
    • >TC015727 RA
      ttgattttg acagtcgcg gcatcatca agcccaaat 
      attttcgtt ttttcgtgc tttttttac caaaaatcc 
      ggtgcataa gcccttaaa aacatccct tttatcaat 
      tcaaccctt cgtgggctt ctagtgcat gaattgcgt 
      caaccatga ccgacgaga ggctccaca ttgactggg 
      gtaacgata agttgcacc gcacccaga agcaggtcg 
      agcgcaacc cctacgacc tcgaatcat ggtcgattt 
      tgctgcgcg aggtgcaaa ccaagcaca tttcggaag 
      tgcgccccc tttacgagc acctcatcg acatattcc 
      ccagcgcgt cgcgctact ggcgcatct acatcgagc 
      acgagatga agtcccgga atttcgaaa gagtggaaa 
      agttgtttc aaaggtgtt tgatgaaga tcttaaata 
      ttgagttgt ggaaacttt atctcggat atgttaaag 
      aaaccaagg cttcgctcc cgacctata aggaaaaaa 
      tggcacaag cctacgact ttgccttgg acaaaatcg 
      gaatggaca tccattcgt actcgattt ggaacgaat 
      acgtgaatt ttttaaagg gggtggagg ccgtgggct 
      cgtatgcgg agaaccaga aaatttcag cggtgcgta 
      aggtctacc aaaggggca ttaataacc ccatgacgg 
      ggatggaga ccttctgga aggactaca tagccttcg 
      aacaagcca ttaacccca ttattgcgg aaaaaatga 
      gcatcgaga ggagtcgcg attacatga acgcgcgca 
      gagtcgcca aagaactcg aagtacaga ttcggggga 
      ttaacagga acgccccca gtgtgcccc ccaacggaa 
      gcccagaag agaggaaac aagtcgagc tgtggcaga 
      aatatatcg cgtgggaga agagcaatc cgcttcgca 
      ccgaagaca cggccctct tgaccaaac gagtggtct 
      tcgcccttg agcaatgcc tactttgtt tgggacacc 
      accctgata tttggtacc aagcggcac agtttctcg 
      aatacagtt gtaaaattc tagccgaga aaggggacg 
      tcaacgctt cgaaattat ttagcgaag aggcggcaa 
      atatgttcg agagggcga cgagctcgc tcttgaaca 
      aaaacatgc tactttact tcgcctacg ctgattacg 
      aggaagggc gtttgaaat acgaaaaag tccaccaga 
      tttaccaaa agtaccttg aaattcaag atatcgacc 
      cgacacttg cgtacatcc agtatatga aattcgcca 
      gacgggccg aagggatca agtcggctc gggccgttt 
      tcaaaagag cgcgcgaag acaatcgct ccaagtacc 
      acattttcg tctgcgccg ccttgatgg agtactatt 
      gcagtaaag acaaaaaca tcgcgtttc gaattttcg 
      aactgggtt tgaaaaaat tcggggata tccccgaat 
      atatcacgt gttacatcg actatttgt cacacttga 
      acgaagaca acaacacgc gagtgctat tcgagcgcg 
      tcctgtcat ctggaagtc tagaaccgg aaaaatccg 
      ttgacattt ggaaccgtt ttctggaat tcgagtgca 
      atatcgggg atttacaga gtattgtca aagttgaga 
      aaagacgat cggaagtct tgtcaaaaa ttaaagaat 
      ttgaaggca aagaaacgg cacaattgg tcgacagat 
      acaaatttt tagatttgt atccctgca cggccacgg 
      aactcaaaa gtatagggt atacagaag tgatcaaca 
      taacggggg cggggcgca accacattt tacagtcga 
      taatcaacc tcgacggcg acgccccct tgcccctgc 
      ccgactact cacaaatga tcccctaca agcccaagt 
      ccaaccccc ttccgggcg agcatccgg tccccggcg 
      gtgcgttcc cccaccctc cggtggcgg cgtacctgt 
      gcacgcttc tgccgcccc cggcatgtt tccgggggc 
      ctttcgtca acgtggacc tcctcatgg acattttca 
      accggatcc agttaccgg attcgcccc cgcagccgt 
      cggaaaatg ggggcgatg tgaggcttt ttgatttgg 
      caaaatccg ttcattgga tcgtcgagg aagggggcg 
      gagtcggga ttaagagga agaagagac tgggattgg 
      ctatggatg ggtcagatg atgaggatt tgccgctgc 
      cgccgacca atgatattt acaggcagc ggcaacaga 
      agcgtgtga aatgattta attgtgctt tgttcattg 
      tgacttgtt taagtatag aataaattg atttcatta 
      gg