| General information | |||
| TC015723 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007421.3: 5761047..5763676 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC015723 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02508 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 22.22% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 66.67% (includes death as larva, prepupa, pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
remarks: animals lost,
- >TC015723 RA
MLRSCVLFL IFSCAFASH YDTWPFTLA DKYIEEFKT NVEEFKKFA HTYQHHEEI KYEVEEQDL TEATKNAAH YDFIIVGGG TSGAILASR LSEIPEWKI LLLEAGAPE TIATKVPKN WELLKNTPY NWGYVTTPQ NYSCLGMVD HKCVIPTGR ALGGTTSIN SMVYTRGNP RDYDLWSDL GNEGWCWAD VLPYYKKLE DAHFAPFDK KYHHFGGPQ HLEHPQYLR FLTDHTLEA AKELDLHLI DYNGKHQIG ISVPQLTSK CGKRFSTAE AYLERAEKR DNLIVKPLS QVLKVLIST HTKEAQGVV YLHEGKTFV AKAEKEVVL AAGALNTPK ILLLSGVGP KEDCEKLHI HHVADLKVG HNLKIRPSF VGLDFLYTA EEAQSHDEY HDILKYLKY GKGPLTSPG IEALAFLKT NISKSPLTY PDIELKFLS RYHPQQDLY SWMKPTPKH YDSLWKPLE AHNCLKIIV TLNHPKSSG IVKLHTSNP LRPPIIEPH FLSDEDEKD YHTILAGIK KALKFSHTE AFKKIGIKL NHHGVHGCE ETEFGTEAY WECAIKYLV VATEDVSGT ARMGPESDH YAVVDKKLR VHGIHNLRV ADASVIPVT MSGSLVGPT MVIGEKAAH IIMEEWLEH
- >TC015723 RA
atgttacgg tcgtgtgtt ctcttcttg atcttttct tgtgccttt gcatcgcat tatgacact tggccgttt acactagcg gataaatac atcgaagaa ttcaaaaca aatgtcgaa gaattcaaa aaattcgca cacacgtat cagcaccat gaagaaatt aaatatgag gtggaggaa caagatctg accgaagcg acgaaaaat gcggctcat tatgacttc atcatagtc ggcggtggt acttccggt gccatttta gcatcccga ttatccgaa atccccgaa tggaaaatc ctacttttg gaagccggc gcacccgaa acaatcgca accaaagtc cccaaaaat tgggaacta ctgaaaaac accccctat aattggggg tacgttact accccccag aactactcg tgtctagga atggttgac cacaaatgt gtcatccca actggtcgg gccttgggt ggtaccacc tccatcaac tccatggta tacacccgt gggaacccc cgcgactac gacttgtgg tccgacctc ggaaacgaa ggctggtgt tgggccgac gttctccca tattacaag aaattggaa gatgcccat tttgcgccc tttgataag aagtaccac cactttggc ggcccccaa cacctcgaa cacccccaa tacttgcgc tttttgacc gaccatacg ctcgaagcg gccaaagaa ctcgactta cacctcata gattacaac ggaaagcac cagattggg ataagtgta ccccaacta acttcaaag tgtggcaaa agattcagt accgctgaa gcttatctc gaacgcgcc gaaaaacgc gacaacctc atagtcaaa cctttgagc caagttctg aaagtcttg atcagcact cacaccaaa gaagcccaa ggcgttgtt tatttacac gaaggcaaa actttcgta gccaaggct gagaaagaa gtcgtactt gcagctggt gccttgaac accccgaaa attttacta ctgtcagga gtaggtcct aaggaagat tgtgaaaag ctccacatt catcacgtg gctgattta aaagtgggg cataattta aaaatacgg ccaagcttc gtcggtttg gacttcttg tacacggca gaagaagcc caaagtcat gacgagtac cacgacatc ctcaagtac ctcaaatac gggaaagga ccgctaaca tcccccgga atcgaggct ttggctttc ctcaaaacc aacatttct aaatcaccc ttaacgtac cccgacatt gagttaaag ttcctctcg cggtaccac ccccagcaa gacctctac tcctggatg aagcccacc cccaaacac tacgactcg ctctggaag cccctcgaa gcccacaat tgcctcaaa atcatagtc acactcaac caccccaaa tcgtcaggg attgtcaaa ctgcacacc tccaacccc ctccgcccc ccaatcata gaaccacat ttcttgtca gacgaggac gaaaaggac taccatacg attttagcc gggattaag aaagcactc aagtttagc cacactgaa gctttcaag aaaattggg attaaattg aaccatcat ggggttcat ggctgtgag gagacggag tttggtacc gaggcgtac tgggaatgt gcgattaaa tatttggtg gtggcgacc gaggatgtg agtggtact gcgaggatg gggcctgag agtgaccac tatgcagtg gttgacaag aaattgagg gttcatggg atacataat ttgagggta gctgatgcg agtgtcatc ccagtgacc atgagtgga agcttggta ggacccacg atggttatt ggggaaaaa gcagcccat attattatg gaagagtgg ctagagcat tag
- >TC015723 RA
ttaaagcaa agaggataa accagcact cttcgctac gcggatttc ccacaagaa aaaaacaaa atcttcatt tataaacgc gtgctgagg cgttcttcg catcattcg gtaaaatgt tacggtcgt gtgttctct tcttgatct tttcttgtg cctttgcat cgcattatg acacttggc cgtttacac tagcggata aatacatcg aagaattca aaacaaatg tcgaagaat tcaaaaaat tcgcacaca cgtatcagc accatgaag aaattaaat atgaggtgg aggaacaag atctgaccg aagcgacga aaaatgcgg ctcattatg acttcatca tagtcggcg gtggtactt ccggtgcca ttttagcat cccgattat ccgaaatcc ccgaatgga aaatcctac ttttggaag ccggcgcac ccgaaacaa tcgcaacca aagtcccca aaaattggg aactactga aaaacaccc cctataatt gggggtacg ttactaccc cccagaact actcgtgtc taggaatgg ttgaccaca aatgtgtca tcccaactg gtcgggcct tgggtggta ccacctcca tcaactcca tggtataca cccgtggga acccccgcg actacgact tgtggtccg acctcggaa acgaaggct ggtgttggg ccgacgttc tcccatatt acaagaaat tggaagatg cccattttg cgccctttg ataagaagt accaccact ttggcggcc cccaacacc tcgaacacc cccaatact tgcgctttt tgaccgacc atacgctcg aagcggcca aagaactcg acttacacc tcatagatt acaacggaa agcaccaga ttgggataa gtgtacccc aactaactt caaagtgtg gcaaaagat tcagtaccg ctgaagctt atctcgaac gcgccgaaa aacgcgaca acctcatag tcaaacctt tgagccaag ttctgaaag tcttgatca gcactcaca ccaaagaag cccaaggcg ttgtttatt tacacgaag gcaaaactt tcgtagcca aggctgaga aagaagtcg tacttgcag ctggtgcct tgaacaccc cgaaaattt tactactgt caggagtag gtcctaagg aagattgtg aaaagctcc acattcatc acgtggctg atttaaaag tggggcata atttaaaaa tacggccaa gcttcgtcg gtttggact tcttgtaca cggcagaag aagcccaaa gtcatgacg agtaccacg acatcctca agtacctca aatacggga aaggaccgc taacatccc ccggaatcg aggctttgg ctttcctca aaaccaaca tttctaaat cacccttaa cgtaccccg acattgagt taaagttcc tctcgcggt accaccccc agcaagacc tctactcct ggatgaagc ccaccccca aacactacg actcgctct ggaagcccc tcgaagccc acaattgcc tcaaaatca tagtcacac tcaaccacc ccaaatcgt cagggattg tcaaactgc acacctcca accccctcc gccccccaa tcatagaac cacatttct tgtcagacg aggacgaaa aggactacc atacgattt tagccggga ttaagaaag cactcaagt ttagccaca ctgaagctt tcaagaaaa ttgggatta aattgaacc atcatgggg ttcatggct gtgaggaga cggagtttg gtaccgagg cgtactggg aatgtgcga ttaaatatt tggtggtgg cgaccgagg atgtgagtg gtactgcga ggatggggc ctgagagtg accactatg cagtggttg acaagaaat tgagggttc atgggatac ataatttga gggtagctg atgcgagtg tcatcccag tgaccatga gtggaagct tggtaggac ccacgatgg ttattgggg aaaaagcag cccatatta ttatggaag agtggctag agcattaga atagttttt ttcacagca cttgatctc tgtattctt gacgattaa taaattttt atacgaaag
