Status
Please wait ...
Gene information
General information
TC015723 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 5761047..5763676
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015723
    iBeetle screen iB_02508

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 22.22% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 66.67% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    remarks: animals lost,
    Pupal morphology
    remarks: animals lost,
    Adult morphology
    remarks: animals lost,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015723 RA
      MLRSCVLFL IFSCAFASH YDTWPFTLA DKYIEEFKT 
      NVEEFKKFA HTYQHHEEI KYEVEEQDL TEATKNAAH 
      YDFIIVGGG TSGAILASR LSEIPEWKI LLLEAGAPE 
      TIATKVPKN WELLKNTPY NWGYVTTPQ NYSCLGMVD 
      HKCVIPTGR ALGGTTSIN SMVYTRGNP RDYDLWSDL 
      GNEGWCWAD VLPYYKKLE DAHFAPFDK KYHHFGGPQ 
      HLEHPQYLR FLTDHTLEA AKELDLHLI DYNGKHQIG 
      ISVPQLTSK CGKRFSTAE AYLERAEKR DNLIVKPLS 
      QVLKVLIST HTKEAQGVV YLHEGKTFV AKAEKEVVL 
      AAGALNTPK ILLLSGVGP KEDCEKLHI HHVADLKVG 
      HNLKIRPSF VGLDFLYTA EEAQSHDEY HDILKYLKY 
      GKGPLTSPG IEALAFLKT NISKSPLTY PDIELKFLS 
      RYHPQQDLY SWMKPTPKH YDSLWKPLE AHNCLKIIV 
      TLNHPKSSG IVKLHTSNP LRPPIIEPH FLSDEDEKD 
      YHTILAGIK KALKFSHTE AFKKIGIKL NHHGVHGCE 
      ETEFGTEAY WECAIKYLV VATEDVSGT ARMGPESDH 
      YAVVDKKLR VHGIHNLRV ADASVIPVT MSGSLVGPT 
      MVIGEKAAH IIMEEWLEH
    CDS sequence
    • >TC015723 RA
      atgttacgg tcgtgtgtt ctcttcttg atcttttct 
      tgtgccttt gcatcgcat tatgacact tggccgttt 
      acactagcg gataaatac atcgaagaa ttcaaaaca 
      aatgtcgaa gaattcaaa aaattcgca cacacgtat 
      cagcaccat gaagaaatt aaatatgag gtggaggaa 
      caagatctg accgaagcg acgaaaaat gcggctcat 
      tatgacttc atcatagtc ggcggtggt acttccggt 
      gccatttta gcatcccga ttatccgaa atccccgaa 
      tggaaaatc ctacttttg gaagccggc gcacccgaa 
      acaatcgca accaaagtc cccaaaaat tgggaacta 
      ctgaaaaac accccctat aattggggg tacgttact 
      accccccag aactactcg tgtctagga atggttgac 
      cacaaatgt gtcatccca actggtcgg gccttgggt 
      ggtaccacc tccatcaac tccatggta tacacccgt 
      gggaacccc cgcgactac gacttgtgg tccgacctc 
      ggaaacgaa ggctggtgt tgggccgac gttctccca 
      tattacaag aaattggaa gatgcccat tttgcgccc 
      tttgataag aagtaccac cactttggc ggcccccaa 
      cacctcgaa cacccccaa tacttgcgc tttttgacc 
      gaccatacg ctcgaagcg gccaaagaa ctcgactta 
      cacctcata gattacaac ggaaagcac cagattggg 
      ataagtgta ccccaacta acttcaaag tgtggcaaa 
      agattcagt accgctgaa gcttatctc gaacgcgcc 
      gaaaaacgc gacaacctc atagtcaaa cctttgagc 
      caagttctg aaagtcttg atcagcact cacaccaaa 
      gaagcccaa ggcgttgtt tatttacac gaaggcaaa 
      actttcgta gccaaggct gagaaagaa gtcgtactt 
      gcagctggt gccttgaac accccgaaa attttacta 
      ctgtcagga gtaggtcct aaggaagat tgtgaaaag 
      ctccacatt catcacgtg gctgattta aaagtgggg 
      cataattta aaaatacgg ccaagcttc gtcggtttg 
      gacttcttg tacacggca gaagaagcc caaagtcat 
      gacgagtac cacgacatc ctcaagtac ctcaaatac 
      gggaaagga ccgctaaca tcccccgga atcgaggct 
      ttggctttc ctcaaaacc aacatttct aaatcaccc 
      ttaacgtac cccgacatt gagttaaag ttcctctcg 
      cggtaccac ccccagcaa gacctctac tcctggatg 
      aagcccacc cccaaacac tacgactcg ctctggaag 
      cccctcgaa gcccacaat tgcctcaaa atcatagtc 
      acactcaac caccccaaa tcgtcaggg attgtcaaa 
      ctgcacacc tccaacccc ctccgcccc ccaatcata 
      gaaccacat ttcttgtca gacgaggac gaaaaggac 
      taccatacg attttagcc gggattaag aaagcactc 
      aagtttagc cacactgaa gctttcaag aaaattggg 
      attaaattg aaccatcat ggggttcat ggctgtgag 
      gagacggag tttggtacc gaggcgtac tgggaatgt 
      gcgattaaa tatttggtg gtggcgacc gaggatgtg 
      agtggtact gcgaggatg gggcctgag agtgaccac 
      tatgcagtg gttgacaag aaattgagg gttcatggg 
      atacataat ttgagggta gctgatgcg agtgtcatc 
      ccagtgacc atgagtgga agcttggta ggacccacg 
      atggttatt ggggaaaaa gcagcccat attattatg 
      gaagagtgg ctagagcat tag
    mRNA sequence
    • >TC015723 RA
      ttaaagcaa agaggataa accagcact cttcgctac 
      gcggatttc ccacaagaa aaaaacaaa atcttcatt 
      tataaacgc gtgctgagg cgttcttcg catcattcg 
      gtaaaatgt tacggtcgt gtgttctct tcttgatct 
      tttcttgtg cctttgcat cgcattatg acacttggc 
      cgtttacac tagcggata aatacatcg aagaattca 
      aaacaaatg tcgaagaat tcaaaaaat tcgcacaca 
      cgtatcagc accatgaag aaattaaat atgaggtgg 
      aggaacaag atctgaccg aagcgacga aaaatgcgg 
      ctcattatg acttcatca tagtcggcg gtggtactt 
      ccggtgcca ttttagcat cccgattat ccgaaatcc 
      ccgaatgga aaatcctac ttttggaag ccggcgcac 
      ccgaaacaa tcgcaacca aagtcccca aaaattggg 
      aactactga aaaacaccc cctataatt gggggtacg 
      ttactaccc cccagaact actcgtgtc taggaatgg 
      ttgaccaca aatgtgtca tcccaactg gtcgggcct 
      tgggtggta ccacctcca tcaactcca tggtataca 
      cccgtggga acccccgcg actacgact tgtggtccg 
      acctcggaa acgaaggct ggtgttggg ccgacgttc 
      tcccatatt acaagaaat tggaagatg cccattttg 
      cgccctttg ataagaagt accaccact ttggcggcc 
      cccaacacc tcgaacacc cccaatact tgcgctttt 
      tgaccgacc atacgctcg aagcggcca aagaactcg 
      acttacacc tcatagatt acaacggaa agcaccaga 
      ttgggataa gtgtacccc aactaactt caaagtgtg 
      gcaaaagat tcagtaccg ctgaagctt atctcgaac 
      gcgccgaaa aacgcgaca acctcatag tcaaacctt 
      tgagccaag ttctgaaag tcttgatca gcactcaca 
      ccaaagaag cccaaggcg ttgtttatt tacacgaag 
      gcaaaactt tcgtagcca aggctgaga aagaagtcg 
      tacttgcag ctggtgcct tgaacaccc cgaaaattt 
      tactactgt caggagtag gtcctaagg aagattgtg 
      aaaagctcc acattcatc acgtggctg atttaaaag 
      tggggcata atttaaaaa tacggccaa gcttcgtcg 
      gtttggact tcttgtaca cggcagaag aagcccaaa 
      gtcatgacg agtaccacg acatcctca agtacctca 
      aatacggga aaggaccgc taacatccc ccggaatcg 
      aggctttgg ctttcctca aaaccaaca tttctaaat 
      cacccttaa cgtaccccg acattgagt taaagttcc 
      tctcgcggt accaccccc agcaagacc tctactcct 
      ggatgaagc ccaccccca aacactacg actcgctct 
      ggaagcccc tcgaagccc acaattgcc tcaaaatca 
      tagtcacac tcaaccacc ccaaatcgt cagggattg 
      tcaaactgc acacctcca accccctcc gccccccaa 
      tcatagaac cacatttct tgtcagacg aggacgaaa 
      aggactacc atacgattt tagccggga ttaagaaag 
      cactcaagt ttagccaca ctgaagctt tcaagaaaa 
      ttgggatta aattgaacc atcatgggg ttcatggct 
      gtgaggaga cggagtttg gtaccgagg cgtactggg 
      aatgtgcga ttaaatatt tggtggtgg cgaccgagg 
      atgtgagtg gtactgcga ggatggggc ctgagagtg 
      accactatg cagtggttg acaagaaat tgagggttc 
      atgggatac ataatttga gggtagctg atgcgagtg 
      tcatcccag tgaccatga gtggaagct tggtaggac 
      ccacgatgg ttattgggg aaaaagcag cccatatta 
      ttatggaag agtggctag agcattaga atagttttt 
      ttcacagca cttgatctc tgtattctt gacgattaa 
      taaattttt atacgaaag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY