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Gene information
General information
TC015723 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 5761047..5763676
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC015723
    iBeetle screen iB_02508

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 22.22% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 66.67% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    remarks: animals lost,
    Pupal morphology
    remarks: animals lost,
    Adult morphology
    remarks: animals lost,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015723 RA
      MLRSCVLFL IFSCAFASH YDTWPFTLA DKYIEEFKT 
NVEEFKKFA HTYQHHEEI KYEVEEQDL TEATKNAAH 
YDFIIVGGG TSGAILASR LSEIPEWKI LLLEAGAPE 
TIATKVPKN WELLKNTPY NWGYVTTPQ NYSCLGMVD 
HKCVIPTGR ALGGTTSIN SMVYTRGNP RDYDLWSDL 
GNEGWCWAD VLPYYKKLE DAHFAPFDK KYHHFGGPQ 
HLEHPQYLR FLTDHTLEA AKELDLHLI DYNGKHQIG 
ISVPQLTSK CGKRFSTAE AYLERAEKR DNLIVKPLS 
QVLKVLIST HTKEAQGVV YLHEGKTFV AKAEKEVVL 
AAGALNTPK ILLLSGVGP KEDCEKLHI HHVADLKVG 
HNLKIRPSF VGLDFLYTA EEAQSHDEY HDILKYLKY 
GKGPLTSPG IEALAFLKT NISKSPLTY PDIELKFLS 
RYHPQQDLY SWMKPTPKH YDSLWKPLE AHNCLKIIV 
TLNHPKSSG IVKLHTSNP LRPPIIEPH FLSDEDEKD 
YHTILAGIK KALKFSHTE AFKKIGIKL NHHGVHGCE 
ETEFGTEAY WECAIKYLV VATEDVSGT ARMGPESDH 
YAVVDKKLR VHGIHNLRV ADASVIPVT MSGSLVGPT 
MVIGEKAAH IIMEEWLEH
    CDS sequence
    • >TC015723 RA
      atgttacgg tcgtgtgtt ctcttcttg atcttttct 
tgtgccttt gcatcgcat tatgacact tggccgttt 
acactagcg gataaatac atcgaagaa ttcaaaaca 
aatgtcgaa gaattcaaa aaattcgca cacacgtat 
cagcaccat gaagaaatt aaatatgag gtggaggaa 
caagatctg accgaagcg acgaaaaat gcggctcat 
tatgacttc atcatagtc ggcggtggt acttccggt 
gccatttta gcatcccga ttatccgaa atccccgaa 
tggaaaatc ctacttttg gaagccggc gcacccgaa 
acaatcgca accaaagtc cccaaaaat tgggaacta 
ctgaaaaac accccctat aattggggg tacgttact 
accccccag aactactcg tgtctagga atggttgac 
cacaaatgt gtcatccca actggtcgg gccttgggt 
ggtaccacc tccatcaac tccatggta tacacccgt 
gggaacccc cgcgactac gacttgtgg tccgacctc 
ggaaacgaa ggctggtgt tgggccgac gttctccca 
tattacaag aaattggaa gatgcccat tttgcgccc 
tttgataag aagtaccac cactttggc ggcccccaa 
cacctcgaa cacccccaa tacttgcgc tttttgacc 
gaccatacg ctcgaagcg gccaaagaa ctcgactta 
cacctcata gattacaac ggaaagcac cagattggg 
ataagtgta ccccaacta acttcaaag tgtggcaaa 
agattcagt accgctgaa gcttatctc gaacgcgcc 
gaaaaacgc gacaacctc atagtcaaa cctttgagc 
caagttctg aaagtcttg atcagcact cacaccaaa 
gaagcccaa ggcgttgtt tatttacac gaaggcaaa 
actttcgta gccaaggct gagaaagaa gtcgtactt 
gcagctggt gccttgaac accccgaaa attttacta 
ctgtcagga gtaggtcct aaggaagat tgtgaaaag 
ctccacatt catcacgtg gctgattta aaagtgggg 
cataattta aaaatacgg ccaagcttc gtcggtttg 
gacttcttg tacacggca gaagaagcc caaagtcat 
gacgagtac cacgacatc ctcaagtac ctcaaatac 
gggaaagga ccgctaaca tcccccgga atcgaggct 
ttggctttc ctcaaaacc aacatttct aaatcaccc 
ttaacgtac cccgacatt gagttaaag ttcctctcg 
cggtaccac ccccagcaa gacctctac tcctggatg 
aagcccacc cccaaacac tacgactcg ctctggaag 
cccctcgaa gcccacaat tgcctcaaa atcatagtc 
acactcaac caccccaaa tcgtcaggg attgtcaaa 
ctgcacacc tccaacccc ctccgcccc ccaatcata 
gaaccacat ttcttgtca gacgaggac gaaaaggac 
taccatacg attttagcc gggattaag aaagcactc 
aagtttagc cacactgaa gctttcaag aaaattggg 
attaaattg aaccatcat ggggttcat ggctgtgag 
gagacggag tttggtacc gaggcgtac tgggaatgt 
gcgattaaa tatttggtg gtggcgacc gaggatgtg 
agtggtact gcgaggatg gggcctgag agtgaccac 
tatgcagtg gttgacaag aaattgagg gttcatggg 
atacataat ttgagggta gctgatgcg agtgtcatc 
ccagtgacc atgagtgga agcttggta ggacccacg 
atggttatt ggggaaaaa gcagcccat attattatg 
gaagagtgg ctagagcat tag
    mRNA sequence
    • >TC015723 RA
      ttaaagcaa agaggataa accagcact cttcgctac 
gcggatttc ccacaagaa aaaaacaaa atcttcatt 
tataaacgc gtgctgagg cgttcttcg catcattcg 
gtaaaatgt tacggtcgt gtgttctct tcttgatct 
tttcttgtg cctttgcat cgcattatg acacttggc 
cgtttacac tagcggata aatacatcg aagaattca 
aaacaaatg tcgaagaat tcaaaaaat tcgcacaca 
cgtatcagc accatgaag aaattaaat atgaggtgg 
aggaacaag atctgaccg aagcgacga aaaatgcgg 
ctcattatg acttcatca tagtcggcg gtggtactt 
ccggtgcca ttttagcat cccgattat ccgaaatcc 
ccgaatgga aaatcctac ttttggaag ccggcgcac 
ccgaaacaa tcgcaacca aagtcccca aaaattggg 
aactactga aaaacaccc cctataatt gggggtacg 
ttactaccc cccagaact actcgtgtc taggaatgg 
ttgaccaca aatgtgtca tcccaactg gtcgggcct 
tgggtggta ccacctcca tcaactcca tggtataca 
cccgtggga acccccgcg actacgact tgtggtccg 
acctcggaa acgaaggct ggtgttggg ccgacgttc 
tcccatatt acaagaaat tggaagatg cccattttg 
cgccctttg ataagaagt accaccact ttggcggcc 
cccaacacc tcgaacacc cccaatact tgcgctttt 
tgaccgacc atacgctcg aagcggcca aagaactcg 
acttacacc tcatagatt acaacggaa agcaccaga 
ttgggataa gtgtacccc aactaactt caaagtgtg 
gcaaaagat tcagtaccg ctgaagctt atctcgaac 
gcgccgaaa aacgcgaca acctcatag tcaaacctt 
tgagccaag ttctgaaag tcttgatca gcactcaca 
ccaaagaag cccaaggcg ttgtttatt tacacgaag 
gcaaaactt tcgtagcca aggctgaga aagaagtcg 
tacttgcag ctggtgcct tgaacaccc cgaaaattt 
tactactgt caggagtag gtcctaagg aagattgtg 
aaaagctcc acattcatc acgtggctg atttaaaag 
tggggcata atttaaaaa tacggccaa gcttcgtcg 
gtttggact tcttgtaca cggcagaag aagcccaaa 
gtcatgacg agtaccacg acatcctca agtacctca 
aatacggga aaggaccgc taacatccc ccggaatcg 
aggctttgg ctttcctca aaaccaaca tttctaaat 
cacccttaa cgtaccccg acattgagt taaagttcc 
tctcgcggt accaccccc agcaagacc tctactcct 
ggatgaagc ccaccccca aacactacg actcgctct 
ggaagcccc tcgaagccc acaattgcc tcaaaatca 
tagtcacac tcaaccacc ccaaatcgt cagggattg 
tcaaactgc acacctcca accccctcc gccccccaa 
tcatagaac cacatttct tgtcagacg aggacgaaa 
aggactacc atacgattt tagccggga ttaagaaag 
cactcaagt ttagccaca ctgaagctt tcaagaaaa 
ttgggatta aattgaacc atcatgggg ttcatggct 
gtgaggaga cggagtttg gtaccgagg cgtactggg 
aatgtgcga ttaaatatt tggtggtgg cgaccgagg 
atgtgagtg gtactgcga ggatggggc ctgagagtg 
accactatg cagtggttg acaagaaat tgagggttc 
atgggatac ataatttga gggtagctg atgcgagtg 
tcatcccag tgaccatga gtggaagct tggtaggac 
ccacgatgg ttattgggg aaaaagcag cccatatta 
ttatggaag agtggctag agcattaga atagttttt 
ttcacagca cttgatctc tgtattctt gacgattaa 
taaattttt atacgaaag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY