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Gene information
General information
TC015702 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid43: 377818..382620
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015702
    iBeetle screen iB_02507
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015702 RA
      MDAIQVTND EVVKVRKYF KVKEDDIKR DVRIIIEWI 
      RQQQHLPDI DDENFIERC LLRNKFRVE QTKQKLDGY 
      CTLRGVHLN MFEDWKSII PSRELTTTL PLPKLTPNL 
      ERVIVWKLR SSDPNDYDI ISIVKYNLL LFEVLIRND 
      YAVGDRFVA DFTGHTAAT IARNNPLII SKFLSLYQG 
      AYSGRFVGC DFINAPPFI NLLLSILKK LLPSKLYGR 
      IRIHKDLKS FHKVVPKEC LPRDYGGDL DTVENLIVK 
      WDKVFEHNQ PIFEKNLRM LSNENLRLG TNKDNSDMF 
      GVAGTFKKL NLD
    CDS sequence
    • >TC015702 RA
      atggacgcg atacaagtc accaacgac gaggttgtc 
      aaagtgagg aaatatttt aaggtgaaa gaggacgat 
      attaaaagg gatgttaga ataataatc gagtggata 
      cggcaacaa cagcattta ccggatatc gacgatgaa 
      aatttcatc gaacggtgt ttgttgcgg aataagttt 
      cgagtcgag caaactaag caaaaacta gacgggtat 
      tgtacgctt agaggggtg catcttaat atgttcgag 
      gactggaag tccattata ccctccaga gagttgacc 
      acaaccttg ccccttccg aagctgacc ccaaacctg 
      gagcgcgtc atagtgtgg aagctgcgt tcgtccgac 
      cccaacgac tacgacata atcagcatt gttaaatac 
      aaccttctc ctattcgaa gtgttgatc cggaacgac 
      tacgccgtg ggcgaccgc ttcgtcgcc gacttcacc 
      ggccacacg gccgccacc atcgcccgc aacaacccc 
      ctaattatc tcaaaattc ttatcactc taccagggc 
      gcctattca ggccgattc gtcggctgc gatttcatc 
      aacgctcct ccttttatc aacctttta ctctcaata 
      ctgaaaaaa ttacttccg tctaagttg tatggaagg 
      atccgcatt cacaaggat ttgaaatcg ttccataaa 
      gtggtgccg aaagaatgc ctgccgaga gattacggc 
      ggtgatctt gacaccgtg gagaactta atcgtcaag 
      tgggacaaa gtgttcgaa cacaaccaa ccgattttc 
      gagaaaaac ctaagaatg ctctccaac gagaatctc 
      aggctgggc accaacaag gacaacagt gacatgttc 
      ggagtcgcc ggaacgttc aaaaaactc aacctcgac 
      tga
    mRNA sequence
    • >TC015702 RA
      atcaagtac acatgcact caaccacca attatcgca 
      agaaacagt ctgtgttta ctgacctac cagcgaaaa 
      caacacaag cacaatcgt caaagctca aacattgac 
      aaaatctcg aattggatt gaaaaaaaa aatcgtgcg 
      gtggtgtca ttccgcccc cgtctcggt gcagatccg 
      ccacgagac atcgtccac tatcaaatg tttggcttc 
      tgcagataa caccactga ccccaaaat atggtaaat 
      aattgggaa ttctttgtt catcgttgc cacaattaa 
      caatggacg cgatacaag tcaccaacg acgaggttg 
      tcaaagtga ggaaatatt ttaaggtga aagaggacg 
      atattaaaa gggatgtta gaataataa tcgagtgga 
      tacggcaac aacagcatt taccggata tcgacgatg 
      aaaatttca tcgaacggt gtttgttgc ggaataagt 
      ttcgagtcg agcaaacta agcaaaaac tagacgggt 
      attgtacgc ttagagggg tgcatctta atatgttcg 
      aggactgga agtccatta taccctcca gagagttga 
      ccacaacct tgccccttc cgaagctga ccccaaacc 
      tggagcgcg tcatagtgt ggaagctgc gttcgtccg 
      accccaacg actacgaca taatcagca ttgttaaat 
      acaaccttc tcctattcg aagtgttga tccggaacg 
      actacgccg tgggcgacc gcttcgtcg ccgacttca 
      ccggccaca cggccgcca ccatcgccc gcaacaacc 
      ccctaatta tctcaaaat tcttatcac tctaccagg 
      gcgcctatt caggccgat tcgtcggct gcgatttca 
      tcaacgctc ctcctttta tcaaccttt tactctcaa 
      tactgaaaa aattacttc cgtctaagt tgtatggaa 
      ggatccgca ttcacaagg atttgaaat cgttccata 
      aagtggtgc cgaaagaat gcctgccga gagattacg 
      gcggtgatc ttgacaccg tggagaact taatcgtca 
      agtgggaca aagtgttcg aacacaacc aaccgattt 
      tcgagaaaa acctaagaa tgctctcca acgagaatc 
      tcaggctgg gcaccaaca aggacaaca gtgacatgt 
      tcggagtcg ccggaacgt tcaaaaaac tcaacctcg 
      actgactgt atatctcac taaatcaat caacaaagt 
      gaaaatttc gcgttttat ttccacctc tcccgtaaa 
      actgtccca gccgtgata aagataatt tgattattt 
      catttctga gcgatataa tcagtttaa attatattt 
      aatgcataa attctgtta acagcgcac tgtacagta 
      atatttaag agaaacttt ggactaact cgatttggg 
      tagttaata aagctcaac tttcggt