Status
Please wait ...
Gene information
General information
TC015675 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 5160724..5162345
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015675
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02505

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality),
    Pupal morphology
    beetle stock: bad (increased lethality),
    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 2
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection



    larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection



    Pupal morphology
    wings slightly shortened - Number of animals showing the phenotype: 3

    oblique dorsal thoracic muscles median slightlythinner - Number of animals showing the phenotype: 2

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015675 RA
      MAPTKNKPP KSGLNPGFF TMENLKKFI YDPHYLKPT 
      CFLLFLFEI ILNIFIIER VNYTEIDWK AYMQEVEGF 
      MNGTWDYKE LKGDTGPLV YPAGFVYVY SLLYAVTSQ 
      GRNIYLAQY IFLVLYLGQ TLLTYRIFL KTAKIPPYA 
      LVLTTLTSY RIHSIYVLR LFNDPIAVL LFYISLNLF 
      ISNRWLIGS FFYSLAVSI KMNILLYAP CLLIAYLTN 
      LTLMETILN LSICALVQV ILGAPFLYT NPFSYIKGS 
      FDLGRVFEH TWTVNYRFL PREIFENRL LHLGLLSCH 
      VLLLLAFAP LLKRYLSSY AKLKIVTDQ VRTQLIDEK 
      LKRQKEDEQ MSQEQRAFL ASFEKQIKG GKTREDNES 
      KLEDKLSKV TQLFVLPFF VTNLIGIVF ARSLHYQFY 
      CWYFHSLLY LVFCTGFRR PVMFLLLGV LEYCWNVYP 
      STDLSSASL HLCHFVLLF GVYRTMRQ
    CDS sequence
    • >TC015675 RA
      atggctccg actaaaaat aagccgccc aaatccggg 
      ctaaacccc ggatttttc acaatggaa aatttaaaa 
      aaattcatc tacgacccc cattacttg aaaccgacg 
      tgtttcctc ctcttcctg ttcgaaatc attctgaac 
      attttcatt attgaaaga gtgaactac acggaaatt 
      gactggaaa gcgtacatg caagaggtt gaggggttt 
      atgaacggg acttgggac tacaaggag ttgaaaggt 
      gacacgggc ccccttgtt tacccggcc ggtttcgtc 
      tacgtgtac tcactttta tacgccgtt acgtcacaa 
      gggcgcaac atttatttg gcccaatac attttcctc 
      gtgttatat ttggggcaa acgctccta acgtaccga 
      attttccta aaaacggcc aaaatcccc ccttatgca 
      ctcgttttg accactttg acctcatac cggattcac 
      tcgatttac gttttgcgc cttttcaac gacccaatt 
      gctgtgctc ctcttttac atcagtctg aatctattt 
      atttcaaac cggtggctt ataggcagt tttttctac 
      tccctagcc gtctcaatc aaaatgaac atcctactg 
      tacgcccct tgtctccta attgcgtat ttaaccaac 
      cttacatta atggaaaca attttaaat ttatcaatt 
      tgcgctttg gtacaagta atactgggg gcccccttt 
      ttatacacc aaccctttt tcgtacatt aagggcagt 
      ttcgatcta ggccgcgtg ttcgagcac acgtggacc 
      gtcaattac cgtttccta ccgcgcgaa attttcgaa 
      aatcgtttg ctacatttg gggctttta tcgtgtcat 
      gttttactc ctcctggcc tttgccccc cttttgaaa 
      cgctatctg agcagttac gcgaaatta aaaatagta 
      acagatcaa gttaggacg caacttatt gacgaaaaa 
      ctcaaaagg caaaaagag gacgaacaa atgagtcag 
      gaacaacgg gcgtttttg gcaagtttt gagaaacaa 
      atcaaaggt gggaaaaca agggaagat aatgaatca 
      aagttggag gataagttg agtaaagtt acccaactg 
      ttcgtttta ccgtttttt gttacgaat ttgattggg 
      attgttttt gcacggtcg ctacattat cagttttat 
      tgttggtat tttcatagt ttgttgtat ttggttttt 
      tgtacgggg tttaggcgc cctgttatg tttctattg 
      ttgggggtt ttggagtat tgttggaat gtttatccc 
      agtacggac ctgtcaagt gcctcgtta catttatgc 
      cattttgtt ctgttattt ggtgtttat aggaccatg 
      agacaataa
    mRNA sequence
    • >TC015675 RA
      ccttggttt ggaaaaatg tagcaatct agtgaattt 
      aatttctcg aaattggcc gcattgctg ttaacctca 
      aaaatgtgt atccccaaa atcagctca cgatggctc 
      cgactaaaa ataagccgc ccaaatccg ggctaaacc 
      ccggatttt tcacaatgg aaaatttaa aaaaattca 
      tctacgacc cccattact tgaaaccga cgtgtttcc 
      tcctcttcc tgttcgaaa tcattctga acattttca 
      ttattgaaa gagtgaact acacggaaa ttgactgga 
      aagcgtaca tgcaagagg ttgaggggt ttatgaacg 
      ggacttggg actacaagg agttgaaag gtgacacgg 
      gcccccttg tttacccgg ccggtttcg tctacgtgt 
      actcacttt tatacgccg ttacgtcac aagggcgca 
      acatttatt tggcccaat acattttcc tcgtgttat 
      atttggggc aaacgctcc taacgtacc gaattttcc 
      taaaaacgg ccaaaatcc ccccttatg cactcgttt 
      tgaccactt tgacctcat accggattc actcgattt 
      acgttttgc gccttttca acgacccaa ttgctgtgc 
      tcctctttt acatcagtc tgaatctat ttatttcaa 
      accggtggc ttataggca gttttttct actccctag 
      ccgtctcaa tcaaaatga acatcctac tgtacgccc 
      cttgtctcc taattgcgt atttaacca accttacat 
      taatggaaa caattttaa atttatcaa tttgcgctt 
      tggtacaag taatactgg gggccccct ttttataca 
      ccaaccctt tttcgtaca ttaagggca gtttcgatc 
      taggccgcg tgttcgagc acacgtgga ccgtcaatt 
      accgtttcc taccgcgcg aaattttcg aaaatcgtt 
      tgctacatt tggggcttt tatcgtgtc atgttttac 
      tcctcctgg cctttgccc cccttttga aacgctatc 
      tgagcagtt acgcgaaat taaaaatag taacagatc 
      aagttagga cgcaactta ttgacgaaa aactcaaaa 
      ggcaaaaag aggacgaac aaatgagtc aggaacaac 
      gggcgtttt tggcaagtt ttgagaaac aaatcaaag 
      gtgggaaaa caagggaag ataatgaat caaagttgg 
      aggataagt tgagtaaag ttacccaac tgttcgttt 
      taccgtttt ttgttacga atttgattg ggattgttt 
      ttgcacggt cgctacatt atcagtttt attgttggt 
      attttcata gtttgttgt atttggttt tttgtacgg 
      ggtttaggc gccctgtta tgtttctat tgttggggg 
      ttttggagt attgttgga atgtttatc ccagtacgg 
      acctgtcaa gtgcctcgt tacatttat gccattttg 
      ttctgttat ttggtgttt ataggacca tgagacaat 
      aaatttttc gttaaacaa aggttttat ttctccgct 
      ctcaccttt atcattatc attattatc actctcttt 
      cagtaaaaa ataatttaa cagactctg t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY