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Gene information
General information
TC015670 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 5200763..5218559
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC015670
    iBeetle screen iB_02504

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Pupal morphology
    beetle stock: bad (increased lethality),
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015670 RA
      MSPLSLVGI ILVTLSPSL ADVSHVLDG SAHPPDPQA 
VNHLATPEQ YWWMNSNSP LKAAYDYYK KCNAKGNCI 
TPINPDAAP QPFQPQPHP QPLDVKHNP FLNGKYNPQ 
ENTAKLNEG GNIDIAKNP FLSGKVVTS GGGDAVVKG 
QDGFLGVQP AQPFKKPGF ATTHPLDAK KKPGFATTH 
PLEKTSKKT GVECKGAGF GCVEKKLCV NGVVNKNGE 
GLLQTRSDV HYCTDEEVC CQLNNLNEI TQGKSSVPG 
STIKFAGGA GFSPGFIHV QPLNVQPDI VFAGEHGHH 
SSSSTSKSI RDTSLSPNS VFSSTVDYS DTKFDSDVR 
FASTFQGPA YLPPVAPTT PKPKPFEQS SIDNHPQPP 
YRPSPSIPQ PSPQPRPRP TSPPFRPTP SIPQFPSPS 
TPQYTPAPP QPQQPTGGY EYPKPTPTF GYPTPRPGG 
SSSNVNNQY VPPPKKPTE GDQSSDAAN LDGTILRPT 
TPRAQFQGE LNTAPPPGC AAALKCVQE IYCTAEGVI 
SPVPVVLTK EQELSRVPT TVCQDTESG IIGKCCRDP 
NYKDPWPSA NLVNGVDDG QYKEDDSIG QYSPDLQRN 
VRSETGGPR QDASNINVN VQQPQPKCG ERHPNTSPK 
GPGPLDVNF AEIPWQAMV LRDSNRSLL CGGAIIRRN 
AVITAAHCV EGLETSDIL VKGGEWKLG IDEEPLPFQ 
IVKVAVVVR HPQYQPGSF VNDLALLVL EEKLRPSKN 
IGTLCLPPP NQIPTENCI ATGWGKRIL QLHAKGAIM 
HSINVNVMD NQQCQETLK SKFQHAVGN HSPNTLCGY 
SDIDQCKVD YGSAMACYK DGGYTLSGI YAWDTGCKQ 
EGQIGGYVA PDVDWIEST LAKPITELK KLEH
    CDS sequence
    • >TC015670 RA
      atgtctcca ctatcccta gtggggata atcctagtc 
accctctcc ccctccctg gcggacgtc tcccacgtc 
ctggacggc tcagcccac ccccctgac ccccaagcc 
gtcaaccat ctcgccacc cctgaacag tactggtgg 
atgaactca aactcaccc ctcaaagcc gcctacgac 
tactacaaa aaatgcaac gccaaaggc aactgcatc 
acgcccata aacccagac gccgcccca caacccttc 
caaccccag ccccacccc cagccgctc gacgtcaaa 
cacaacccc ttcctcaac ggtaaatac aacccccag 
gaaaacacc gccaaatta aacgaaggc ggtaacatt 
gacattgcg aaaaacccg tttttgagc gggaaagtt 
gtgaccagt ggcggcggc gacgcagtc gtcaaagga 
caggatggt ttcctgggg gtgcaaccg gcacagccc 
ttcaaaaaa ccgggattt gcaaccaca catcctttg 
gatgcgaag aaaaaacct ggatttgct acaactcac 
cccttggag aagacgtcc aagaaaaca ggggtggag 
tgcaagggg gcgggtttt ggttgcgtc gaaaaaaag 
ttgtgcgtt aatggagtg gtgaataaa aatggagaa 
ggattgttg cagacgagg agtgatgtg cattactgt 
accgacgag gaagtctgc tgccaactc aacaacctg 
aacgaaatc acgcaagga aagagctca gttccgggc 
tcaacgatt aaattcgcc ggaggcgcc ggattcagt 
cccggattc atccacgtt caaccattg aacgtccaa 
ccagatatt gttttcgca ggagaacat ggacaccac 
tcaagctca agtacctca aaatcgatc agagatacc 
tcattgagc cccaattca gtgttctca agcacagtc 
gattatagc gacacgaag tttgatagc gatgtaaga 
ttcgcttcg actttccaa ggaccggct tatctaccc 
ccagtcgcc ccaactacc cctaaaccc aaacccttc 
gaacagtcg agcatcgac aaccacccc caaccccca 
taccgacca agtccttca attcctcaa ccaagtccc 
cagccacgc ccacgtccg acctctccc ccattccgc 
ccaactccc tccataccc caattccca tcaccctca 
actccacaa tacacacca gcgccaccg caacctcaa 
caaccaacc ggcggttac gagtaccct aaaccgacc 
ccaactttc gggtacccc acaccacga ccgggcggt 
tccagcagc aacgtcaat aatcagtat gtaccccct 
cccaaaaaa cccacagaa ggtgaccaa agttccgat 
gctgccaac cttgatggt actatttta cgtccaacg 
accccccga gcccaattc caaggcgaa ctcaacaca 
gccccacca ccaggatgt gcagctgct cttaaatgt 
gtacaagaa atttactgt acagcagaa ggggtcatt 
tctccagta ccagtagta ctaacaaaa gaacaagaa 
ttgagccga gtaccgacg acggtgtgt caagacacc 
gaatcgggt atcatcggg aagtgttgt cgcgacccc 
aattataaa gatccatgg ccgtctgcg aatctcgta 
aatggggta gacgacgga caatacaaa gaagacgac 
agtatcggg caatattca cccgattta caaagaaac 
gtacgatcg gagacaggg ggcccacga caggatgcc 
tcaaatatc aacgtaaat gtacaacag ccccaacca 
aaatgtggg gaaaggcac ccaaatacc agcccgaag 
ggcccgggc ccccttgat gtcaatttc gccgaaata 
ccatggcag gccatggtt ttgcgcgac tccaatcgc 
agtcttttg tgcgggggg gcgataatc aggcgcaat 
gctgtcatc accgccgca cactgcgtt gaagggctt 
gaaacgagc gatattttg gtgaaaggg ggcgaatgg 
aagctcggt attgacgaa gagcctcta cctttccaa 
attgttaag gtggcagtg gtggtgcgt catccacag 
taccaacct ggttccttc gtcaatgat ttagccctt 
ttggttttg gaggagaaa ctaagaccg tcgaaaaat 
atcggaact ttgtgcctt cctccgcct aatcaaatt 
ccgaccgaa aattgcata gccaccggt tggggcaaa 
agaatctta caacttcac gccaaaggt gcgattatg 
cattcgata aacgtcaat gttatggat aatcagcaa 
tgtcaagag actctaaag agcaaattc caacacgct 
gttgggaat cacagcccg aatacgctg tgtggctac 
tcggacatc gaccagtgc aaggtggac tatggtagt 
gcaatggct tgctacaag gacggtggc tacaccctc 
tcggggatt tacgcgtgg gacactggg tgtaaacaa 
gaggggcaa atcggtggt tatgtggcc cccgacgtt 
gattggatc gaatcgacg ttagctaag ccaatcaca 
gagctcaaa aaactcgaa cattag
    mRNA sequence
    • >TC015670 RA
      ggatcagtg tcactcttc gccgtgtga ttgtgatcg 
cgaagtgtg ctgtggaat tacgaagca aaatgtctc 
cactatccc tagtgggga taatcctag tcaccctct 
ccccctccc tggcggacg tctcccacg tcctggacg 
gctcagccc acccccctg acccccaag ccgtcaacc 
atctcgcca cccctgaac agtactggt ggatgaact 
caaactcac ccctcaaag ccgcctacg actactaca 
aaaaatgca acgccaaag gcaactgca tcacgccca 
taaacccag acgccgccc cacaaccct tccaacccc 
agccccacc cccagccgc tcgacgtca aacacaacc 
ccttcctca acggtaaat acaaccccc aggaaaaca 
ccgccaaat taaacgaag gcggtaaca ttgacattg 
cgaaaaacc cgtttttga gcgggaaag ttgtgacca 
gtggcggcg gcgacgcag tcgtcaaag gacaggatg 
gtttcctgg gggtgcaac cggcacagc ccttcaaaa 
aaccgggat ttgcaacca cacatcctt tggatgcga 
agaaaaaac ctggatttg ctacaactc accccttgg 
agaagacgt ccaagaaaa caggggtgg agtgcaagg 
gggcgggtt ttggttgcg tcgaaaaaa agttgtgcg 
ttaatggag tggtgaata aaaatggag aaggattgt 
tgcagacga ggagtgatg tgcattact gtaccgacg 
aggaagtct gctgccaac tcaacaacc tgaacgaaa 
tcacgcaag gaaagagct cagttccgg gctcaacga 
ttaaattcg ccggaggcg ccggattca gtcccggat 
tcatccacg ttcaaccat tgaacgtcc aaccagata 
ttgttttcg caggagaac atggacacc actcaagct 
caagtacct caaaatcga tcagagata cctcattga 
gccccaatt cagtgttct caagcacag tcgattata 
gcgacacga agtttgata gcgatgtaa gattcgctt 
cgactttcc aaggaccgg cttatctac ccccagtcg 
ccccaacta cccctaaac ccaaaccct tcgaacagt 
cgagcatcg acaaccacc cccaacccc cataccgac 
caagtcctt caattcctc aaccaagtc cccagccac 
gcccacgtc cgacctctc ccccattcc gcccaactc 
cctccatac cccaattcc catcaccct caactccac 
aatacacac cagcgccac cgcaacctc aacaaccaa 
ccggcggtt acgagtacc ctaaaccga ccccaactt 
tcgggtacc ccacaccac gaccgggcg gttccagca 
gcaacgtca ataatcagt atgtacccc ctcccaaaa 
aacccacag aaggtgacc aaagttccg atgctgcca 
accttgatg gtactattt tacgtccaa cgacccccc 
gagcccaat tccaaggcg aactcaaca cagccccac 
caccaggat gtgcagctg ctcttaaat gtgtacaag 
aaatttact gtacagcag aaggggtca tttctccag 
taccagtag tactaacaa aagaacaag aattgagcc 
gagtaccga cgacggtgt gtcaagaca ccgaatcgg 
gtatcatcg ggaagtgtt gtcgcgacc ccaattata 
aagatccat ggccgtctg cgaatctcg taaatgggg 
tagacgacg gacaataca aagaagacg acagtatcg 
ggcaatatt cacccgatt tacaaagaa acgtacgat 
cggagacag ggggcccac gacaggatg cctcaaata 
tcaacgtaa atgtacaac agccccaac caaaatgtg 
gggaaaggc acccaaata ccagcccga agggcccgg 
gcccccttg atgtcaatt tcgccgaaa taccatggc 
aggccatgg ttttgcgcg actccaatc gcagtcttt 
tgtgcgggg gggcgataa tcaggcgca atgctgtca 
tcaccgccg cacactgcg ttgaagggc ttgaaacga 
gcgatattt tggtgaaag ggggcgaat ggaagctcg 
gtattgacg aagagcctc tacctttcc aaattgtta 
aggtggcag tggtggtgc gtcatccac agtaccaac 
ctggttcct tcgtcaatg atttagccc ttttggttt 
tggaggaga aactaagac cgtcgaaaa atatcggaa 
ctttgtgcc ttcctccgc ctaatcaaa ttccgaccg 
aaaattgca tagccaccg gttggggca aaagaatct 
tacaacttc acgccaaag gtgcgatta tgcattcga 
taaacgtca atgttatgg ataatcagc aatgtcaag 
agactctaa agagcaaat tccaacacg ctgttggga 
atcacagcc cgaatacgc tgtgtggct actcggaca 
tcgaccagt gcaaggtgg actatggta gtgcaatgg 
cttgctaca aggacggtg gctacaccc tctcgggga 
tttacgcgt gggacactg ggtgtaaac aagaggggc 
aaatcggtg gttatgtgg cccccgacg ttgattgga 
tcgaatcga cgttagcta agccaatca cagagctca 
aaaaactcg aacattagt gagatttaa taagacgag 
gtgaattaa tcagggatg tgttgcgag ggttaaagt 
atcgaaaac tgccatatt ggaccgtgt aatataaat 
ctaattgta attatttga ttttttatt taagtgtaa 
tactgtact aggttagat attatttat aaccgactt 
ggtaaataa aattatttt aatata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY