| General information | |||
| TC015670 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007421.3: 5200763..5218559 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC015670 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Reference | * {{ blurReferenceResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02504 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)
adult lethality - Number of animals showing the phenotype: 2 (22 dpi)
- >TC015670 RA
MSPLSLVGI ILVTLSPSL ADVSHVLDG SAHPPDPQA VNHLATPEQ YWWMNSNSP LKAAYDYYK KCNAKGNCI TPINPDAAP QPFQPQPHP QPLDVKHNP FLNGKYNPQ ENTAKLNEG GNIDIAKNP FLSGKVVTS GGGDAVVKG QDGFLGVQP AQPFKKPGF ATTHPLDAK KKPGFATTH PLEKTSKKT GVECKGAGF GCVEKKLCV NGVVNKNGE GLLQTRSDV HYCTDEEVC CQLNNLNEI TQGKSSVPG STIKFAGGA GFSPGFIHV QPLNVQPDI VFAGEHGHH SSSSTSKSI RDTSLSPNS VFSSTVDYS DTKFDSDVR FASTFQGPA YLPPVAPTT PKPKPFEQS SIDNHPQPP YRPSPSIPQ PSPQPRPRP TSPPFRPTP SIPQFPSPS TPQYTPAPP QPQQPTGGY EYPKPTPTF GYPTPRPGG SSSNVNNQY VPPPKKPTE GDQSSDAAN LDGTILRPT TPRAQFQGE LNTAPPPGC AAALKCVQE IYCTAEGVI SPVPVVLTK EQELSRVPT TVCQDTESG IIGKCCRDP NYKDPWPSA NLVNGVDDG QYKEDDSIG QYSPDLQRN VRSETGGPR QDASNINVN VQQPQPKCG ERHPNTSPK GPGPLDVNF AEIPWQAMV LRDSNRSLL CGGAIIRRN AVITAAHCV EGLETSDIL VKGGEWKLG IDEEPLPFQ IVKVAVVVR HPQYQPGSF VNDLALLVL EEKLRPSKN IGTLCLPPP NQIPTENCI ATGWGKRIL QLHAKGAIM HSINVNVMD NQQCQETLK SKFQHAVGN HSPNTLCGY SDIDQCKVD YGSAMACYK DGGYTLSGI YAWDTGCKQ EGQIGGYVA PDVDWIEST LAKPITELK KLEH
- >TC015670 RA
atgtctcca ctatcccta gtggggata atcctagtc accctctcc ccctccctg gcggacgtc tcccacgtc ctggacggc tcagcccac ccccctgac ccccaagcc gtcaaccat ctcgccacc cctgaacag tactggtgg atgaactca aactcaccc ctcaaagcc gcctacgac tactacaaa aaatgcaac gccaaaggc aactgcatc acgcccata aacccagac gccgcccca caacccttc caaccccag ccccacccc cagccgctc gacgtcaaa cacaacccc ttcctcaac ggtaaatac aacccccag gaaaacacc gccaaatta aacgaaggc ggtaacatt gacattgcg aaaaacccg tttttgagc gggaaagtt gtgaccagt ggcggcggc gacgcagtc gtcaaagga caggatggt ttcctgggg gtgcaaccg gcacagccc ttcaaaaaa ccgggattt gcaaccaca catcctttg gatgcgaag aaaaaacct ggatttgct acaactcac cccttggag aagacgtcc aagaaaaca ggggtggag tgcaagggg gcgggtttt ggttgcgtc gaaaaaaag ttgtgcgtt aatggagtg gtgaataaa aatggagaa ggattgttg cagacgagg agtgatgtg cattactgt accgacgag gaagtctgc tgccaactc aacaacctg aacgaaatc acgcaagga aagagctca gttccgggc tcaacgatt aaattcgcc ggaggcgcc ggattcagt cccggattc atccacgtt caaccattg aacgtccaa ccagatatt gttttcgca ggagaacat ggacaccac tcaagctca agtacctca aaatcgatc agagatacc tcattgagc cccaattca gtgttctca agcacagtc gattatagc gacacgaag tttgatagc gatgtaaga ttcgcttcg actttccaa ggaccggct tatctaccc ccagtcgcc ccaactacc cctaaaccc aaacccttc gaacagtcg agcatcgac aaccacccc caaccccca taccgacca agtccttca attcctcaa ccaagtccc cagccacgc ccacgtccg acctctccc ccattccgc ccaactccc tccataccc caattccca tcaccctca actccacaa tacacacca gcgccaccg caacctcaa caaccaacc ggcggttac gagtaccct aaaccgacc ccaactttc gggtacccc acaccacga ccgggcggt tccagcagc aacgtcaat aatcagtat gtaccccct cccaaaaaa cccacagaa ggtgaccaa agttccgat gctgccaac cttgatggt actatttta cgtccaacg accccccga gcccaattc caaggcgaa ctcaacaca gccccacca ccaggatgt gcagctgct cttaaatgt gtacaagaa atttactgt acagcagaa ggggtcatt tctccagta ccagtagta ctaacaaaa gaacaagaa ttgagccga gtaccgacg acggtgtgt caagacacc gaatcgggt atcatcggg aagtgttgt cgcgacccc aattataaa gatccatgg ccgtctgcg aatctcgta aatggggta gacgacgga caatacaaa gaagacgac agtatcggg caatattca cccgattta caaagaaac gtacgatcg gagacaggg ggcccacga caggatgcc tcaaatatc aacgtaaat gtacaacag ccccaacca aaatgtggg gaaaggcac ccaaatacc agcccgaag ggcccgggc ccccttgat gtcaatttc gccgaaata ccatggcag gccatggtt ttgcgcgac tccaatcgc agtcttttg tgcgggggg gcgataatc aggcgcaat gctgtcatc accgccgca cactgcgtt gaagggctt gaaacgagc gatattttg gtgaaaggg ggcgaatgg aagctcggt attgacgaa gagcctcta cctttccaa attgttaag gtggcagtg gtggtgcgt catccacag taccaacct ggttccttc gtcaatgat ttagccctt ttggttttg gaggagaaa ctaagaccg tcgaaaaat atcggaact ttgtgcctt cctccgcct aatcaaatt ccgaccgaa aattgcata gccaccggt tggggcaaa agaatctta caacttcac gccaaaggt gcgattatg cattcgata aacgtcaat gttatggat aatcagcaa tgtcaagag actctaaag agcaaattc caacacgct gttgggaat cacagcccg aatacgctg tgtggctac tcggacatc gaccagtgc aaggtggac tatggtagt gcaatggct tgctacaag gacggtggc tacaccctc tcggggatt tacgcgtgg gacactggg tgtaaacaa gaggggcaa atcggtggt tatgtggcc cccgacgtt gattggatc gaatcgacg ttagctaag ccaatcaca gagctcaaa aaactcgaa cattag
- >TC015670 RA
ggatcagtg tcactcttc gccgtgtga ttgtgatcg cgaagtgtg ctgtggaat tacgaagca aaatgtctc cactatccc tagtgggga taatcctag tcaccctct ccccctccc tggcggacg tctcccacg tcctggacg gctcagccc acccccctg acccccaag ccgtcaacc atctcgcca cccctgaac agtactggt ggatgaact caaactcac ccctcaaag ccgcctacg actactaca aaaaatgca acgccaaag gcaactgca tcacgccca taaacccag acgccgccc cacaaccct tccaacccc agccccacc cccagccgc tcgacgtca aacacaacc ccttcctca acggtaaat acaaccccc aggaaaaca ccgccaaat taaacgaag gcggtaaca ttgacattg cgaaaaacc cgtttttga gcgggaaag ttgtgacca gtggcggcg gcgacgcag tcgtcaaag gacaggatg gtttcctgg gggtgcaac cggcacagc ccttcaaaa aaccgggat ttgcaacca cacatcctt tggatgcga agaaaaaac ctggatttg ctacaactc accccttgg agaagacgt ccaagaaaa caggggtgg agtgcaagg gggcgggtt ttggttgcg tcgaaaaaa agttgtgcg ttaatggag tggtgaata aaaatggag aaggattgt tgcagacga ggagtgatg tgcattact gtaccgacg aggaagtct gctgccaac tcaacaacc tgaacgaaa tcacgcaag gaaagagct cagttccgg gctcaacga ttaaattcg ccggaggcg ccggattca gtcccggat tcatccacg ttcaaccat tgaacgtcc aaccagata ttgttttcg caggagaac atggacacc actcaagct caagtacct caaaatcga tcagagata cctcattga gccccaatt cagtgttct caagcacag tcgattata gcgacacga agtttgata gcgatgtaa gattcgctt cgactttcc aaggaccgg cttatctac ccccagtcg ccccaacta cccctaaac ccaaaccct tcgaacagt cgagcatcg acaaccacc cccaacccc cataccgac caagtcctt caattcctc aaccaagtc cccagccac gcccacgtc cgacctctc ccccattcc gcccaactc cctccatac cccaattcc catcaccct caactccac aatacacac cagcgccac cgcaacctc aacaaccaa ccggcggtt acgagtacc ctaaaccga ccccaactt tcgggtacc ccacaccac gaccgggcg gttccagca gcaacgtca ataatcagt atgtacccc ctcccaaaa aacccacag aaggtgacc aaagttccg atgctgcca accttgatg gtactattt tacgtccaa cgacccccc gagcccaat tccaaggcg aactcaaca cagccccac caccaggat gtgcagctg ctcttaaat gtgtacaag aaatttact gtacagcag aaggggtca tttctccag taccagtag tactaacaa aagaacaag aattgagcc gagtaccga cgacggtgt gtcaagaca ccgaatcgg gtatcatcg ggaagtgtt gtcgcgacc ccaattata aagatccat ggccgtctg cgaatctcg taaatgggg tagacgacg gacaataca aagaagacg acagtatcg ggcaatatt cacccgatt tacaaagaa acgtacgat cggagacag ggggcccac gacaggatg cctcaaata tcaacgtaa atgtacaac agccccaac caaaatgtg gggaaaggc acccaaata ccagcccga agggcccgg gcccccttg atgtcaatt tcgccgaaa taccatggc aggccatgg ttttgcgcg actccaatc gcagtcttt tgtgcgggg gggcgataa tcaggcgca atgctgtca tcaccgccg cacactgcg ttgaagggc ttgaaacga gcgatattt tggtgaaag ggggcgaat ggaagctcg gtattgacg aagagcctc tacctttcc aaattgtta aggtggcag tggtggtgc gtcatccac agtaccaac ctggttcct tcgtcaatg atttagccc ttttggttt tggaggaga aactaagac cgtcgaaaa atatcggaa ctttgtgcc ttcctccgc ctaatcaaa ttccgaccg aaaattgca tagccaccg gttggggca aaagaatct tacaacttc acgccaaag gtgcgatta tgcattcga taaacgtca atgttatgg ataatcagc aatgtcaag agactctaa agagcaaat tccaacacg ctgttggga atcacagcc cgaatacgc tgtgtggct actcggaca tcgaccagt gcaaggtgg actatggta gtgcaatgg cttgctaca aggacggtg gctacaccc tctcgggga tttacgcgt gggacactg ggtgtaaac aagaggggc aaatcggtg gttatgtgg cccccgacg ttgattgga tcgaatcga cgttagcta agccaatca cagagctca aaaaactcg aacattagt gagatttaa taagacgag gtgaattaa tcagggatg tgttgcgag ggttaaagt atcgaaaac tgccatatt ggaccgtgt aatataaat ctaattgta attatttga ttttttatt taagtgtaa tactgtact aggttagat attatttat aaccgactt ggtaaataa aattatttt aatata
