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Gene information
General information
TC015670 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid42: 661202..678998
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015670
    iBeetle screen iB_02504
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Pupal morphology
    beetle stock: bad (increased lethality),
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015670 RA
      MSPLSLVGI ILVTLSPSL ADVSHVLDG SAHPPDPQA 
      VNHLATPEQ YWWMNSNSP LKAAYDYYK KCNAKGNCI 
      TPINPDAAP QPFQPQPHP QPLDVKHNP FLNGKYNPQ 
      ENTAKLNEG GNIDIAKNP FLSGKVVTS GGGDAVVKG 
      QDGFLGVQP AQPFKKPGF ATTHPLDAK KKPGFATTH 
      PLEKTSKKT GVECKGAGF GCVEKKLCV NGVVNKNGE 
      GLLQTRSDV HYCTDEEVC CQLNNLNEI TQGKSSVPG 
      STIKFAGGA GFSPGFIHV QPLNVQPDI VFAGEHGHH 
      SSSSTSKSI RDTSLSPNS VFSSTVDYS DTKFDSDVR 
      FASTFQGPA YLPPVAPTT PKPKPFEQS SIDNHPQPP 
      YRPSPSIPQ PSPQPRPRP TSPPFRPTP SIPQFPSPS 
      TPQYTPAPP QPQQPTGGY EYPKPTPTF GYPTPRPGG 
      SSSNVNNQY VPPPKKPTE GDQSSDAAN LDGTILRPT 
      TPRAQFQGE LNTAPPPGC AAALKCVQE IYCTAEGVI 
      SPVPVVLTK EQELSRVPT TVCQDTESG IIGKCCRDP 
      NYKDPWPSA NLVNGVDDG QYKEDDSIG QYSPDLQRN 
      VRSETGGPR QDASNINVN VQQPQPKCG ERHPNTSPK 
      GPGPLDVNF AEIPWQAMV LRDSNRSLL CGGAIIRRN 
      AVITAAHCV EGLETSDIL VKGGEWKLG IDEEPLPFQ 
      IVKVAVVVR HPQYQPGSF VNDLALLVL EEKLRPSKN 
      IGTLCLPPP NQIPTENCI ATGWGKRIL QLHAKGAIM 
      HSINVNVMD NQQCQETLK SKFQHAVGN HSPNTLCGY 
      SDIDQCKVD YGSAMACYK DGGYTLSGI YAWDTGCKQ 
      EGQIGGYVA PDVDWIEST LAKPITELK KLEH
    CDS sequence
    • >TC015670 RA
      atgtctcca ctatcccta gtggggata atcctagtc 
      accctctcc ccctccctg gcggacgtc tcccacgtc 
      ctggacggc tcagcccac ccccctgac ccccaagcc 
      gtcaaccat ctcgccacc cctgaacag tactggtgg 
      atgaactca aactcaccc ctcaaagcc gcctacgac 
      tactacaaa aaatgcaac gccaaaggc aactgcatc 
      acgcccata aacccagac gccgcccca caacccttc 
      caaccccag ccccacccc cagccgctc gacgtcaaa 
      cacaacccc ttcctcaac ggtaaatac aacccccag 
      gaaaacacc gccaaatta aacgaaggc ggtaacatt 
      gacattgcg aaaaacccg tttttgagc gggaaagtt 
      gtgaccagt ggcggcggc gacgcagtc gtcaaagga 
      caggatggt ttcctgggg gtgcaaccg gcacagccc 
      ttcaaaaaa ccgggattt gcaaccaca catcctttg 
      gatgcgaag aaaaaacct ggatttgct acaactcac 
      cccttggag aagacgtcc aagaaaaca ggggtggag 
      tgcaagggg gcgggtttt ggttgcgtc gaaaaaaag 
      ttgtgcgtt aatggagtg gtgaataaa aatggagaa 
      ggattgttg cagacgagg agtgatgtg cattactgt 
      accgacgag gaagtctgc tgccaactc aacaacctg 
      aacgaaatc acgcaagga aagagctca gttccgggc 
      tcaacgatt aaattcgcc ggaggcgcc ggattcagt 
      cccggattc atccacgtt caaccattg aacgtccaa 
      ccagatatt gttttcgca ggagaacat ggacaccac 
      tcaagctca agtacctca aaatcgatc agagatacc 
      tcattgagc cccaattca gtgttctca agcacagtc 
      gattatagc gacacgaag tttgatagc gatgtaaga 
      ttcgcttcg actttccaa ggaccggct tatctaccc 
      ccagtcgcc ccaactacc cctaaaccc aaacccttc 
      gaacagtcg agcatcgac aaccacccc caaccccca 
      taccgacca agtccttca attcctcaa ccaagtccc 
      cagccacgc ccacgtccg acctctccc ccattccgc 
      ccaactccc tccataccc caattccca tcaccctca 
      actccacaa tacacacca gcgccaccg caacctcaa 
      caaccaacc ggcggttac gagtaccct aaaccgacc 
      ccaactttc gggtacccc acaccacga ccgggcggt 
      tccagcagc aacgtcaat aatcagtat gtaccccct 
      cccaaaaaa cccacagaa ggtgaccaa agttccgat 
      gctgccaac cttgatggt actatttta cgtccaacg 
      accccccga gcccaattc caaggcgaa ctcaacaca 
      gccccacca ccaggatgt gcagctgct cttaaatgt 
      gtacaagaa atttactgt acagcagaa ggggtcatt 
      tctccagta ccagtagta ctaacaaaa gaacaagaa 
      ttgagccga gtaccgacg acggtgtgt caagacacc 
      gaatcgggt atcatcggg aagtgttgt cgcgacccc 
      aattataaa gatccatgg ccgtctgcg aatctcgta 
      aatggggta gacgacgga caatacaaa gaagacgac 
      agtatcggg caatattca cccgattta caaagaaac 
      gtacgatcg gagacaggg ggcccacga caggatgcc 
      tcaaatatc aacgtaaat gtacaacag ccccaacca 
      aaatgtggg gaaaggcac ccaaatacc agcccgaag 
      ggcccgggc ccccttgat gtcaatttc gccgaaata 
      ccatggcag gccatggtt ttgcgcgac tccaatcgc 
      agtcttttg tgcgggggg gcgataatc aggcgcaat 
      gctgtcatc accgccgca cactgcgtt gaagggctt 
      gaaacgagc gatattttg gtgaaaggg ggcgaatgg 
      aagctcggt attgacgaa gagcctcta cctttccaa 
      attgttaag gtggcagtg gtggtgcgt catccacag 
      taccaacct ggttccttc gtcaatgat ttagccctt 
      ttggttttg gaggagaaa ctaagaccg tcgaaaaat 
      atcggaact ttgtgcctt cctccgcct aatcaaatt 
      ccgaccgaa aattgcata gccaccggt tggggcaaa 
      agaatctta caacttcac gccaaaggt gcgattatg 
      cattcgata aacgtcaat gttatggat aatcagcaa 
      tgtcaagag actctaaag agcaaattc caacacgct 
      gttgggaat cacagcccg aatacgctg tgtggctac 
      tcggacatc gaccagtgc aaggtggac tatggtagt 
      gcaatggct tgctacaag gacggtggc tacaccctc 
      tcggggatt tacgcgtgg gacactggg tgtaaacaa 
      gaggggcaa atcggtggt tatgtggcc cccgacgtt 
      gattggatc gaatcgacg ttagctaag ccaatcaca 
      gagctcaaa aaactcgaa cattag
    mRNA sequence
    • >TC015670 RA
      ggatcagtg tcactcttc gccgtgtga ttgtgatcg 
      cgaagtgtg ctgtggaat tacgaagca aaatgtctc 
      cactatccc tagtgggga taatcctag tcaccctct 
      ccccctccc tggcggacg tctcccacg tcctggacg 
      gctcagccc acccccctg acccccaag ccgtcaacc 
      atctcgcca cccctgaac agtactggt ggatgaact 
      caaactcac ccctcaaag ccgcctacg actactaca 
      aaaaatgca acgccaaag gcaactgca tcacgccca 
      taaacccag acgccgccc cacaaccct tccaacccc 
      agccccacc cccagccgc tcgacgtca aacacaacc 
      ccttcctca acggtaaat acaaccccc aggaaaaca 
      ccgccaaat taaacgaag gcggtaaca ttgacattg 
      cgaaaaacc cgtttttga gcgggaaag ttgtgacca 
      gtggcggcg gcgacgcag tcgtcaaag gacaggatg 
      gtttcctgg gggtgcaac cggcacagc ccttcaaaa 
      aaccgggat ttgcaacca cacatcctt tggatgcga 
      agaaaaaac ctggatttg ctacaactc accccttgg 
      agaagacgt ccaagaaaa caggggtgg agtgcaagg 
      gggcgggtt ttggttgcg tcgaaaaaa agttgtgcg 
      ttaatggag tggtgaata aaaatggag aaggattgt 
      tgcagacga ggagtgatg tgcattact gtaccgacg 
      aggaagtct gctgccaac tcaacaacc tgaacgaaa 
      tcacgcaag gaaagagct cagttccgg gctcaacga 
      ttaaattcg ccggaggcg ccggattca gtcccggat 
      tcatccacg ttcaaccat tgaacgtcc aaccagata 
      ttgttttcg caggagaac atggacacc actcaagct 
      caagtacct caaaatcga tcagagata cctcattga 
      gccccaatt cagtgttct caagcacag tcgattata 
      gcgacacga agtttgata gcgatgtaa gattcgctt 
      cgactttcc aaggaccgg cttatctac ccccagtcg 
      ccccaacta cccctaaac ccaaaccct tcgaacagt 
      cgagcatcg acaaccacc cccaacccc cataccgac 
      caagtcctt caattcctc aaccaagtc cccagccac 
      gcccacgtc cgacctctc ccccattcc gcccaactc 
      cctccatac cccaattcc catcaccct caactccac 
      aatacacac cagcgccac cgcaacctc aacaaccaa 
      ccggcggtt acgagtacc ctaaaccga ccccaactt 
      tcgggtacc ccacaccac gaccgggcg gttccagca 
      gcaacgtca ataatcagt atgtacccc ctcccaaaa 
      aacccacag aaggtgacc aaagttccg atgctgcca 
      accttgatg gtactattt tacgtccaa cgacccccc 
      gagcccaat tccaaggcg aactcaaca cagccccac 
      caccaggat gtgcagctg ctcttaaat gtgtacaag 
      aaatttact gtacagcag aaggggtca tttctccag 
      taccagtag tactaacaa aagaacaag aattgagcc 
      gagtaccga cgacggtgt gtcaagaca ccgaatcgg 
      gtatcatcg ggaagtgtt gtcgcgacc ccaattata 
      aagatccat ggccgtctg cgaatctcg taaatgggg 
      tagacgacg gacaataca aagaagacg acagtatcg 
      ggcaatatt cacccgatt tacaaagaa acgtacgat 
      cggagacag ggggcccac gacaggatg cctcaaata 
      tcaacgtaa atgtacaac agccccaac caaaatgtg 
      gggaaaggc acccaaata ccagcccga agggcccgg 
      gcccccttg atgtcaatt tcgccgaaa taccatggc 
      aggccatgg ttttgcgcg actccaatc gcagtcttt 
      tgtgcgggg gggcgataa tcaggcgca atgctgtca 
      tcaccgccg cacactgcg ttgaagggc ttgaaacga 
      gcgatattt tggtgaaag ggggcgaat ggaagctcg 
      gtattgacg aagagcctc tacctttcc aaattgtta 
      aggtggcag tggtggtgc gtcatccac agtaccaac 
      ctggttcct tcgtcaatg atttagccc ttttggttt 
      tggaggaga aactaagac cgtcgaaaa atatcggaa 
      ctttgtgcc ttcctccgc ctaatcaaa ttccgaccg 
      aaaattgca tagccaccg gttggggca aaagaatct 
      tacaacttc acgccaaag gtgcgatta tgcattcga 
      taaacgtca atgttatgg ataatcagc aatgtcaag 
      agactctaa agagcaaat tccaacacg ctgttggga 
      atcacagcc cgaatacgc tgtgtggct actcggaca 
      tcgaccagt gcaaggtgg actatggta gtgcaatgg 
      cttgctaca aggacggtg gctacaccc tctcgggga 
      tttacgcgt gggacactg ggtgtaaac aagaggggc 
      aaatcggtg gttatgtgg cccccgacg ttgattgga 
      tcgaatcga cgttagcta agccaatca cagagctca 
      aaaaactcg aacattagt gagatttaa taagacgag 
      gtgaattaa tcagggatg tgttgcgag ggttaaagt 
      atcgaaaac tgccatatt ggaccgtgt aatataaat 
      ctaattgta attatttga ttttttatt taagtgtaa 
      tactgtact aggttagat attatttat aaccgactt 
      ggtaaataa aattatttt aatata