Status
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Gene information
General information
TC015663 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 4457107..4466763
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015663
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02503

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    previtellogenic egg chamber structure irregular - Number of animals showing the phenotype: 3

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015663 RA
      MATDSQQRC PKIMVFRPT WDEFKDFSQ YIQHMESKG 
      AHKAGLAKV IPPPEWVPR KSGYAIDTL DVTIPAPIC 
      QVVTGKQGL YQQINIQKK SMTVQQYCE LANSERYAT 
      PRHFDYEDL ERKYWKNIT YVAPIYGAD VSGSLTDDT 
      VNEWNINRL GTILDYVNE DYGISIEGV NTAYLYFGM 
      WKTTFAWHT EDMDLYSIN YLHFGAPKT WYSIPPEHG 
      RRLERLANG FFPSSYKTC QAFLRHKMT LISPQILKQ 
      YSIPYNKIT QEAGEIMIT FPYGYHAGF NHGFNCAES 
      TNFACERWI EYGKRASQC TCSKDMVKI SMDTFVKRF 
      QADRYEMWL KGEDIGPHP EEPNKQVAA PLPLPQDIL 
      CNKNNDSLP AGLIEGQLK KAKKAGRMM NYSQDYSMV 
      DFPAELQLQ LMEEDDMGY VNGEFPPDE QQLEVLEDI 
      WLKAGEIEA EDASICDAG YRVTNRRKF LAKKRKPKE 
      PRPRGRPPK KRTKDGEEE PESNELTGI YAPCNSQSK 
      KVCGQIVAP VDTSELVKS LVAQETNKL LHHKKHKHK 
      HKKCKHHQK TATQTSPDT ANSEVKKEI DDIIRKAHE 
      EHEQTIMQQ EEKPPVKPS LLSELKAYR KVKDPTDQF 
      RMEPKVQTI HTSKGTITV LESRPVVPP PPETKPPER 
      KSLYEDAFL NFLTAKTDK PVPDLAGGS MPNLSEPEP 
      VLIERSEQM PKLEPVPVE EAPTVQVNP NPVPPTYYQ 
      RKPNLIWSN HKYYTVNHC TTTTYLNND RFYPMTNNP 
      IVLLEKATP KTPDSDSGS DCSCESCSC SECDEQVEN 
      SSNSINVVP QNKKLNLEQ LKQLTQRVL GVENGTKFY 
      NDKIVALKK MLTMRRRGR PPKNNSLEK RNNNNTMTD 
      FHSYNCKLL EKIAKLTPL EAKVKLVDV LNNFTNDVQ 
      NYLKAGLPL ANVSALSLD EPPPNDETS ERFPVKLNE 
      FVWAKHLNG RFYKAKIVD IKIVTYFCV YFPLDQSFC 
      KDVELADLV GWDLKKVPI VGQKLRVRW KDGTICVAE 
      FLGKNNSSI FTVMFEDES RLELHRDSI VAASESISK 
      KIASLK
    CDS sequence
    • >TC015663 RA
      atggccaca gactcgcaa caacgctgt cccaagatt 
      atggtcttt cggcccact tgggacgag tttaaggac 
      ttcagtcag tatatccag cacatggag tccaagggg 
      gcgcacaag gccggcttg gcgaaagtg attccgccc 
      cccgagtgg gtcccccgc aagtcgggg tatgccatt 
      gacacgctc gacgtcacg attccggcc cccatttgc 
      caggtggtt accggaaaa caggggctt taccagcaa 
      atcaacatc cagaaaaaa tcaatgacc gtccagcaa 
      tactgcgag ttggccaac tcggaacgc tatgccacg 
      ccgagacac ttcgactac gaggacttg gagcgcaaa 
      tactggaag aatatcacg tacgtggcc cccatttac 
      ggggccgat gtctcgggg agtctcacc gatgatacg 
      gtcaacgag tggaatatc aaccgtctg gggacaatc 
      cttgactac gttaatgag gattatggg atttcgata 
      gaaggcgtc aatacggcc tatttatat ttcgggatg 
      tggaaaaca acatttgca tggcacacg gaagatatg 
      gacttgtac tcaatcaat tatctacac tttggggcc 
      ccgaaaact tggtattct attccgccg gaacatgga 
      cgacgattg gagcgtcta gctaatggt tttttcccg 
      agcagttac aagacgtgt caggcgttt ttacggcat 
      aagatgacg ctgatttcg ccccaaatc ttgaaacag 
      tactcgatt ccgtataat aagattacg caagaggcg 
      ggcgagatt atgatcact tttccgtac ggatatcat 
      gccggtttc aaccatggt tttaactgt gccgaatcg 
      acaaacttt gcctgcgaa cgttggatc gaatatggc 
      aaaagagcc tcccaatgc acttgcagt aaagacatg 
      gtaaaaatt tcaatggac actttcgtc aagcgcttc 
      caagccgat cgttacgaa atgtggcta aaaggcgaa 
      gacatcggc ccccatccg gaagaacca aacaaacaa 
      gttgccgca cctttaccc ctcccacaa gacatcctc 
      tgtaataaa aataatgat tctttgccg gcaggactg 
      atcgaaggc caattgaaa aaggccaaa aaagccggc 
      aggatgatg aattattct caggattat tcaatggtt 
      gatttcccg gctgaacta cagctccaa ctgatggaa 
      gaggacgat atgggttac gttaacggg gagttcccc 
      cccgatgaa caacaattg gaagttttg gaagatatt 
      tggctgaaa gctggcgaa atcgaagcc gaagatgcg 
      tcaatctgt gatgccggt tatcgtgtc actaatcgt 
      cgaaaattc ctagcgaaa aaacgcaaa ccaaaggaa 
      ccgcgtccg cgcggtcgc ccaccgaaa aaacgcacc 
      aaagacggc gaagaagaa cccgaatcg aacgaactg 
      accggcatt tacgccccg tgtaacagc caaagtaag 
      aaagtctgc ggtcaaatt gtcgcacct gttgacaca 
      tccgaactc gtcaagtca ctcgtggcg caagaaacc 
      aacaaactc ctccaccac aaaaaacac aaacacaag 
      cataagaag tgcaaacac caccaaaaa accgcaaca 
      cagaccagc cctgacact gcaaacagc gaagtcaag 
      aaagaaatc gacgacatc atccgcaaa gcacacgaa 
      gaacatgaa caaacaatc atgcaacaa gaggaaaaa 
      ccaccggtg aaacccagc ctcttgagc gaactcaaa 
      gcctaccgc aaggtgaaa gacccgacc gaccagttc 
      cgaatggag ccgaaggtg caaaccatc catacgtcg 
      aaaggaacg atcaccgtg ttggaatca cgaccggtg 
      gttccccca ccgcccgaa acgaaacca cccgaacgc 
      aaatccctc tacgaagac gcgttcctc aatttccta 
      acggccaaa accgacaaa cccgtacct gatctagcc 
      ggcggttcg atgccgaat ttaagcgaa cccgagccg 
      gtgcttatc gaacgcagc gaacaaatg cccaagctg 
      gagccggtc ccagtcgag gaggcgcca accgtgcaa 
      gtcaacccg aatccagtg cctccaacc tactaccaa 
      cgtaaaccc aatctcatt tggagcaat cacaagtac 
      tacaccgtc aatcattgc acgaccacc acgtaccta 
      aacaacgac cgcttttac ccaatgaca aacaacccg 
      atcgtgcta ttggagaag gcgacgccg aaaacgccc 
      gattcggat tcgggctcc gattgttcg tgcgagtcg 
      tgttcgtgc agcgaatgt gatgaacag gtggagaac 
      tcgtcaaac agtattaac gtggtgccg caaaataaa 
      aagctcaat ttggagcaa ttgaaacag ttgacacag 
      cgcgtgttg ggcgtggag aacgggacg aagttctac 
      aatgataaa atcgtcgct ttgaagaag atgttgacg 
      atgcgacgg cgtggacga ccgccgaaa aacaactca 
      ctcgagaag aggaataac aacaacaca atgaccgat 
      ttccactcg tacaattgc aaactttta gagaaaatt 
      gccaaactg acgccgctc gaagcgaaa gttaaatta 
      gtcgatgtt ttaaataat ttcaccaac gatgtgcag 
      aattatctc aaggctggg ttaccacta gctaatgtt 
      agtgcattg tcgttggat gagccacca ccgaatgat 
      gagaccagt gaacggttt cctgttaaa ttaaatgag 
      tttgtttgg gccaaacat ttgaacggg cgcttttac 
      aaggcgaag attgttgat ataaaaata gtgacttat 
      ttttgtgtt tacttcccg cttgatcaa agtttttgc 
      aaagacgtt gaattggcg gatttggtt ggttgggat 
      ctgaagaaa gtaccaatc gtaggacag aagttgaga 
      gtcaggtgg aaagatggc acgatttgt gtggctgag 
      tttttagga aaaaacaac agtagcata tttacggtt 
      atgtttgaa gatgagtct cgtttggaa ttgcacagg 
      gactcaatt gttgcagcg tctgaatcg atatcgaag 
      aaaatcgca agtttgaaa tag
    mRNA sequence
    • >TC015663 RA
      cccctcgtg cgcatgcgc cacctcggt agtgttgtg 
      tctcatccg tgtgttttt ttcccgtga aaaagtcgc 
      gaaaaaatg ccaaaaatc actaaaact gccccaaac 
      cccaatcac acgcttcgc ccttttggt ctctagtcg 
      aaaattgcg cgttttttt cgccaaacg gccgttttt 
      tcgcatacg tgcagtcca ctgtgtcaa tgatggcgg 
      cttgacatt tcctcacgc cgtcaacaa atcacaaat 
      tcggcaaaa agtgatgat tttcgtgtg aattaaggc 
      gaaaaaatg gccacagac tcgcaacaa cgctgtccc 
      aagattatg gtctttcgg cccacttgg gacgagttt 
      aaggacttc agtcagtat atccagcac atggagtcc 
      aagggggcg cacaaggcc ggcttggcg aaagtgatt 
      ccgcccccc gagtgggtc ccccgcaag tcggggtat 
      gccattgac acgctcgac gtcacgatt ccggccccc 
      atttgccag gtggttacc ggaaaacag gggctttac 
      cagcaaatc aacatccag aaaaaatca atgaccgtc 
      cagcaatac tgcgagttg gccaactcg gaacgctat 
      gccacgccg agacacttc gactacgag gacttggag 
      cgcaaatac tggaagaat atcacgtac gtggccccc 
      atttacggg gccgatgtc tcggggagt ctcaccgat 
      gatacggtc aacgagtgg aatatcaac cgtctgggg 
      acaatcctt gactacgtt aatgaggat tatgggatt 
      tcgatagaa ggcgtcaat acggcctat ttatatttc 
      gggatgtgg aaaacaaca tttgcatgg cacacggaa 
      gatatggac ttgtactca atcaattat ctacacttt 
      ggggccccg aaaacttgg tattctatt ccgccggaa 
      catggacga cgattggag cgtctagct aatggtttt 
      ttcccgagc agttacaag acgtgtcag gcgttttta 
      cggcataag atgacgctg atttcgccc caaatcttg 
      aaacagtac tcgattccg tataataag attacgcaa 
      gaggcgggc gagattatg atcactttt ccgtacgga 
      tatcatgcc ggtttcaac catggtttt aactgtgcc 
      gaatcgaca aactttgcc tgcgaacgt tggatcgaa 
      tatggcaaa agagcctcc caatgcact tgcagtaaa 
      gacatggta aaaatttca atggacact ttcgtcaag 
      cgcttccaa gccgatcgt tacgaaatg tggctaaaa 
      ggcgaagac atcggcccc catccggaa gaaccaaac 
      aaacaagtt gccgcacct ttacccctc ccacaagac 
      atcctctgt aataaaaat aatgattct ttgccggca 
      ggactgatc gaaggccaa ttgaaaaag gccaaaaaa 
      gccggcagg atgatgaat tattctcag gattattca 
      atggttgat ttcccggct gaactacag ctccaactg 
      atggaagag gacgatatg ggttacgtt aacggggag 
      ttccccccc gatgaacaa caattggaa gttttggaa 
      gatatttgg ctgaaagct ggcgaaatc gaagccgaa 
      gatgcgtca atctgtgat gccggttat cgtgtcact 
      aatcgtcga aaattccta gcgaaaaaa cgcaaacca 
      aaggaaccg cgtccgcgc ggtcgccca ccgaaaaaa 
      cgcaccaaa gacggcgaa gaagaaccc gaatcgaac 
      gaactgacc ggcatttac gccccgtgt aacagccaa 
      agtaagaaa gtctgcggt caaattgtc gcacctgtt 
      gacacatcc gaactcgtc aagtcactc gtggcgcaa 
      gaaaccaac aaactcctc caccacaaa aaacacaaa 
      cacaagcat aagaagtgc aaacaccac caaaaaacc 
      gcaacacag accagccct gacactgca aacagcgaa 
      gtcaagaaa gaaatcgac gacatcatc cgcaaagca 
      cacgaagaa catgaacaa acaatcatg caacaagag 
      gaaaaacca ccggtgaaa cccagcctc ttgagcgaa 
      ctcaaagcc taccgcaag gtgaaagac ccgaccgac 
      cagttccga atggagccg aaggtgcaa accatccat 
      acgtcgaaa ggaacgatc accgtgttg gaatcacga 
      ccggtggtt cccccaccg cccgaaacg aaaccaccc 
      gaacgcaaa tccctctac gaagacgcg ttcctcaat 
      ttcctaacg gccaaaacc gacaaaccc gtacctgat 
      ctagccggc ggttcgatg ccgaattta agcgaaccc 
      gagccggtg cttatcgaa cgcagcgaa caaatgccc 
      aagctggag ccggtccca gtcgaggag gcgccaacc 
      gtgcaagtc aacccgaat ccagtgcct ccaacctac 
      taccaacgt aaacccaat ctcatttgg agcaatcac 
      aagtactac accgtcaat cattgcacg accaccacg 
      tacctaaac aacgaccgc ttttaccca atgacaaac 
      aacccgatc gtgctattg gagaaggcg acgccgaaa 
      acgcccgat tcggattcg ggctccgat tgttcgtgc 
      gagtcgtgt tcgtgcagc gaatgtgat gaacaggtg 
      gagaactcg tcaaacagt attaacgtg gtgccgcaa 
      aataaaaag ctcaatttg gagcaattg aaacagttg 
      acacagcgc gtgttgggc gtggagaac gggacgaag 
      ttctacaat gataaaatc gtcgctttg aagaagatg 
      ttgacgatg cgacggcgt ggacgaccg ccgaaaaac 
      aactcactc gagaagagg aataacaac aacacaatg 
      accgatttc cactcgtac aattgcaaa cttttagag 
      aaaattgcc aaactgacg ccgctcgaa gcgaaagtt 
      aaattagtc gatgtttta aataatttc accaacgat 
      gtgcagaat tatctcaag gctgggtta ccactagct 
      aatgttagt gcattgtcg ttggatgag ccaccaccg 
      aatgatgag accagtgaa cggtttcct gttaaatta 
      aatgagttt gtttgggcc aaacatttg aacgggcgc 
      ttttacaag gcgaagatt gttgatata aaaatagtg 
      acttatttt tgtgtttac ttcccgctt gatcaaagt 
      ttttgcaaa gacgttgaa ttggcggat ttggttggt 
      tgggatctg aagaaagta ccaatcgta ggacagaag 
      ttgagagtc aggtggaaa gatggcacg atttgtgtg 
      gctgagttt ttaggaaaa aacaacagt agcatattt 
      acggttatg tttgaagat gagtctcgt ttggaattg 
      cacagggac tcaattgtt gcagcgtct gaatcgata 
      tcgaagaaa atcgcaagt ttgaaatag gttaggaat 
      gaaatggag aggcaacag tttgataaa taaaaagtg 
      tgtgtaata aaatgcatg aatgcgagc attttgtac 
      acaacgata ggttgttta taatttgtt gtaaagact 
      gaatttttg cgttgaggc atcattatt tcgagtatt 
      ttggacttt ttttttctt tctttcttt gattgcatt 
      gtgattgtt tgatttaat tatgtacgt aaggtttaa 
      gtttaagtg tgatacaaa catttgtat ttatttaac 
      gagtacgtg tacatacag aattctata ttctctaga 
      ttttttcga aacattgta aaggagtag ttgcaagaa 
      gtgttgaat aaaatttct tcacgttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY