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Gene information
General information
TC015659 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid41: 616630..620415
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015659
    iBeetle screen iB_02502
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015659 RA
      MTKEQMRAL ILVGGYGTR LRPLTLSRP KPLVEFANK 
      PILLHQMEA LVEAGVTEV ILAVSYRAE QMEQELKAE 
      AVKLGISLI FSHESEPLG TAGPIALAR EHLLKSTKP 
      FFVLNSDII CDFPFKELA KFHRDHGQE GTIVVTKVE 
      EPSKYGVVV YDEKHCIES FVEKPQEFI SNKINAGLY 
      ILNPSVVNR IQLRPTSIE KEVFPDMVS DQQLYAFEL 
      QGFWMDVGQ PKDFLTGMC LYLKDLRTK NADKLYKGP 
      GVVGNVLVD PSAKIGPNC QIGPNVTIG PGVVIEEGV 
      CVKRSTILR DAVIKSNSW LENCIVGWR CSVGKWVRM 
      EGTTVLGED VIVKDETYI NGGQVLPHK NITVSVPEP 
      QIIM
    CDS sequence
    • >TC015659 RA
      atgacgaaa gagcagatg cgggctttg atccttgtg 
      gggggctac ggcacgcgc ctccgcccc ctgaccctg 
      agccggccc aagcccttg gtggagttc gccaacaag 
      cccatcctc ctccaccag atggaggcc cttgtggag 
      gccggcgtc accgaagtc atcctggcc gtgtcctac 
      agggccgag caaatggaa caggagctg aaagcggag 
      gctgtcaag ctaggcata agtttgatt ttttcccat 
      gaaagcgag cccctgggc actgcgggg cccatcgca 
      ctggcgcgg gagcatctg ctgaagagc acgaagccg 
      ttttttgtc cttaattct gatattatt tgcgatttt 
      cccttcaaa gagttggct aagtttcat agggatcat 
      gggcaggag ggcaccatt gtggtgacc aaggtggag 
      gagccctcc aagtatggg gttgtggtc tatgatgag 
      aagcactgc attgagagc ttcgtggag aagccacag 
      gagtttatt tcgaataaa atcaatgca ggcctctac 
      atcctaaac cccagcgtc gtaaaccgc atccaactc 
      cgtcccacc tccatcgaa aaagaggtc ttccctgac 
      atggtctcc gaccagcaa ctgtacgcc ttcgaactc 
      cagggtttc tggatggac gtcggccaa ccgaaagac 
      ttcctcacc ggaatgtgt ctctatttg aaagatttg 
      cgtacgaaa aacgccgat aagttgtac aaagggcca 
      ggcgttgta ggcaatgtc ctggtggac ccttcggcg 
      aagatcggc cccaactgc cagatcggg cccaatgtg 
      acgataggg cccggagtt gtgattgag gaaggggtt 
      tgtgtgaag cgatcgacg attttgagg gatgcagta 
      attaagtca aactcctgg ctggaaaac tgcatagtg 
      ggctggagg tgttcggtg gggaagtgg gtgcggatg 
      gaggggacg acggttcta ggggaggat gttatcgtc 
      aaggacgaa acgtatatc aatggggga caagtgttg 
      ccgcataaa aacatcacc gtctcggtg cccgagccc 
      cagattatc atgtag
    mRNA sequence
    • >TC015659 RA
      ttgtttaca cgtcacctg tcaagcccg ttgaggtta 
      attgatggt gttatcagt tagatgata ttaaacttg 
      ttgacgtgt cttgccaac gaagatgac gaaagagca 
      gatgcgggc tttgatcct tgtgggggg ctacggcac 
      gcgcctccg ccccctgac cctgagccg gcccaagcc 
      cttggtgga gttcgccaa caagcccat cctcctcca 
      ccagatgga ggcccttgt ggaggccgg cgtcaccga 
      agtcatcct ggccgtgtc ctacagggc cgagcaaat 
      ggaacagga gctgaaagc ggaggctgt caagctagg 
      cataagttt gattttttc ccatgaaag cgagcccct 
      gggcactgc ggggcccat cgcactggc gcgggagca 
      tctgctgaa gagcacgaa gccgttttt tgtccttaa 
      ttctgatat tatttgcga ttttccctt caaagagtt 
      ggctaagtt tcataggga tcatgggca ggagggcac 
      cattgtggt gaccaaggt ggaggagcc ctccaagta 
      tggggttgt ggtctatga tgagaagca ctgcattga 
      gagcttcgt ggagaagcc acaggagtt tatttcgaa 
      taaaatcaa tgcaggcct ctacatcct aaaccccag 
      cgtcgtaaa ccgcatcca actccgtcc cacctccat 
      cgaaaaaga ggtcttccc tgacatggt ctccgacca 
      gcaactgta cgccttcga actccaggg tttctggat 
      ggacgtcgg ccaaccgaa agacttcct caccggaat 
      gtgtctcta tttgaaaga tttgcgtac gaaaaacgc 
      cgataagtt gtacaaagg gccaggcgt tgtaggcaa 
      tgtcctggt ggacccttc ggcgaagat cggccccaa 
      ctgccagat cgggcccaa tgtgacgat agggcccgg 
      agttgtgat tgaggaagg ggtttgtgt gaagcgatc 
      gacgatttt gagggatgc agtaattaa gtcaaactc 
      ctggctgga aaactgcat agtgggctg gaggtgttc 
      ggtggggaa gtgggtgcg gatggaggg gacgacggt 
      tctagggga ggatgttat cgtcaagga cgaaacgta 
      tatcaatgg gggacaagt gttgccgca taaaaacat 
      caccgtctc ggtgcccga gccccagat tatcatgta 
      gttgttgtt atattttgt tttatatat tttttcata 
      tactctgta atgtgcgat aattgatgc catttattt 
      gtttgtaat aaatgattt tttcaaat