Status
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Gene information
General information
TC033413
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC015639 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 3838544..3844972
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC033413
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02500

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection

    Fertility
    ovary size decreased - Number of animals showing the phenotype: 4
    vitellogenic egg chamber number decreased - Number of animals showing the phenotype: 4
    previtellogenic egg chamber pattern irregular - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 11.11% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (41 dpi)
    Days post injection

    Adult morphology
    wings dorsal not closed - Number of animals showing the phenotype: 3


    Fertility
    egg production: number no/almost no eggs -
    egg chamber mostly not present - Number of animals showing the phenotype: 4

    tropharium size decreased - Number of animals showing the phenotype: 4
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033413 RA
      MSVLNQSGE EQVECPLCM EPLEVDDLN FFPCTCGYQ 
ICRFCWHRI RTDENGLCP ACRKAYSED PADFIPLSQ 
EQVAKLKAE KRQRDQQRK AKLSESRKH LASVRVVQK 
NLVFVVGLP MRLAEPEVL KRHEYFGKF GKIHKVVIN 
QSTAYAGSQ GPSASAYVT YMKSDDALR AIESVNNIT 
IDGRLVKSS LGTTKYCSH FMKNQPCPK PDCMYLHDF 
GDPEASFTK EQMHAGKHQ EYEKKLHEA LLQRTNNNN 
TNVTTTETK PTTKSKEPV AAATPTNGT NKDNWPQLN 
SEKENSKEK KAEKSTESK DKVTKGKSK EKSKEKKEK 
KPEVKVAKD KEETKKESG EKLSILDDN TSFFSHNSF 
QKIVPENRE DDDSPNQTS PSLLTDSLP DINTSEDWA 
AAFGFNKTS ETTVRSESS QSPIDSFFR TDDFRNIIE 
ASLTTAKNN GYTKPPQPA TTTTTTSEN YFGKESGLD 
QHMSKFFMD FHTKNGQKE SNGFTMNHN MNGFQVNNY 
FINQDRLFL LQQKKIEEH LVNLSLKQQ NQSYGGFMN 
GDVVAQNGY GGGFQGQMY QNPQPVQKS RTHAEDDLG 
FDPFQETQK ALAELIANE QSYKAHSNV RGGMSSSQM 
NGVVHSQSG KSHLPPPPP GFVQPTTHM NSFGSKILP 
FLNMSNQQI SSSTQQQQQ NWPSYNSQV QPPPPPQQQ 
QKNANSCND WKDFDSAIV TTSRHYPIM GPINNPFRS 
LQQQLGTQQ IPNGSLRSH EFTNNAFSN FTQQLQQQQ 
PSYSNYSSH LNNTTQQQQ QNLSWFTSE LNTQISSPP 
GFRTATQTS KQQEC
    CDS sequence
    • >TC033413 RA
      atgtcagtg ttgaatcaa agcggggaa gagcaagtc 
gagtgcccc ctgtgcatg gagcccctc gaggtggac 
gatttgaac tttttcccg tgcacttgc ggctatcag 
atttgtcgg ttctgctgg cacagaatc cggaccgat 
gaaaacggt ctgtgtcca gcgtgccgc aaggcctac 
tcggaggac cccgccgac ttcatcccc ctgtctcag 
gagcaagtg gcgaagctc aaggccgag aagagacag 
agggatcag cagaggaag gcgaagctg agcgagagc 
aggaaacat ctagcgagt gttagggtc gtgcaaaag 
aatctggtg tttgtggtg gggctgccg atgagatta 
gccgagccc gaggtgctc aaaaggcac gagtatttc 
ggcaagttt ggcaagata cataaagtc gttattaat 
cagtcgacg gcgtatgcg ggctcacag gggcccagc 
gcaagtgcc tacgtgacg tatatgaaa agcgatgac 
gctttacgc gccattgaa agtgtcaat aacataaca 
atcgacgga cgtctcgta aaatccagt ttaggaacg 
acgaaatat tgcagtcac tttatgaaa aatcaacca 
tgtccaaaa ccggactgt atgtacctc catgatttc 
ggagatcct gaagctagt tttacgaaa gagcaaatg 
cacgccggg aaacatcaa gaatacgaa aagaaatta 
cacgaagcg ctgttacaa cggactaat aacaataat 
acgaacgtg acaacaaca gagacaaaa ccgacaaca 
aaaagtaaa gaaccggtg gcggctgcg acgccaacg 
aatggcaca aataaggat aattggcca cagttgaat 
tccgaaaag gagaatagt aaggaaaag aaggcggag 
aagtcgacg gaaagtaaa gataaagtt acgaaaggc 
aaaagtaaa gaaaaatcg aaagagaag aaggagaag 
aagcccgaa gttaaagtc gcaaaggat aaggaagag 
acgaagaaa gagagtgga gagaaatta tcaatattg 
gacgataac acgagtttt ttttcgcac aattcgttc 
caaaaaatc gtccccgaa aatcgagaa gacgacgac 
agtcccaat caaacatca ccgtctctt ctaaccgat 
tctctcccc gacataaac acgagcgaa gattgggcc 
gcagctttc ggttttaat aaaacaagc gagacaaca 
gttcggtcc gaatcgagt caatcgcca atcgattcg 
tttttccgc accgacgat tttcgtaat attatcgaa 
gcctcactt acaacagcc aaaaataac ggttatacc 
aaaccacca caaccagcc accacaaca acaacaaca 
tcggagaat tatttcggt aaagagtcg ggtttggat 
caacacatg tccaagttt tttatggat tttcatacg 
aaaaatggc cagaaagag agtaacggt tttacgatg 
aatcataat atgaacggg tttcaagtg aataattat 
tttatcaat caggatcgt ttgttcttg ttgcaacag 
aaaaaaatc gaggaacat ttagtgaat ctctcgttg 
aagcaacag aatcagagt tatggcggg tttatgaat 
ggtgatgta gtggcacag aatggctat gggggaggg 
tttcaagga cagatgtat cagaatcca caaccagtg 
caaaagagt cggacacat gcggaggac gatttgggc 
tttgatccc ttccaggag acgcagaag gccttggct 
gagcttata gcgaacgaa cagagttat aaggcacat 
agtaatgtt agaggaggt atgtcgtcg agtcaaatg 
aacggtgtg gtccacagt cagagcggt aaaagtcac 
ttacccccg cccccgccc ggttttgta caaccgaca 
acacatatg aattcattc ggtagtaaa attttacca 
tttttaaat atgtcaaat caacaaatt agttcatca 
acacaacaa caacaacaa aactggcct agttataat 
tcgcaagtg cagccgcca ccgccacca caacaacag 
cagaaaaat gcaaacagc tgtaacgat tggaaggat 
ttcgattcg gcaatagtc acaaccagt cgtcactac 
ccaataatg ggaccgata aataatcct ttcagatcg 
ttacaacaa cagttaggt acacaacaa ataccgaac 
ggatcgtta cgatcgcac gaatttacg aataacgca 
ttttcaaat ttcacacaa cagttgcaa caacaacag 
ccgagttat tcaaattat tcatcacat ttaaataat 
acaacacag caacaacaa cagaatctg agttggttt 
actagcgaa ttaaataca caaatatca agtccaccc 
ggatttcga actgcgact caaacgtct aaacaacaa 
gaatgttaa
    mRNA sequence
    • >TC033413 RA
      acattttgt aaacaaagg tttcatccc tgacaagtg 
taaacgtgg ctttagtag gtgaatgtg tttggagcg 
catgcgcaa gcctacgag atcatagtc gccatattt 
ccaatctgt ctaatctga aaattcagt aagtaaatt 
acgttttcg cacggattc gcgtgtttt tgtccgaaa 
tcgccgccg tttctctct caattcccc cgaaattta 
gcgtgatcg cgcaatgtc agtgttgaa tcaaagcgg 
ggaagagca agtcgagtg ccccctgtg catggagcc 
cctcgaggt ggacgattt gaacttttt cccgtgcac 
ttgcggcta tcagatttg tcggttctg ctggcacag 
aatccggac cgatgaaaa cggtctgtg tccagcgtg 
ccgcaaggc ctactcgga ggaccccgc cgacttcat 
ccccctgtc tcaggagca agtggcgaa gctcaaggc 
cgagaagag acagaggga tcagcagag gaaggcgaa 
gctgagcga gagcaggaa acatctagc gagtgttag 
ggtcgtgca aaagaatct ggtgtttgt ggtggggct 
gccgatgag attagccga gcccgaggt gctcaaaag 
gcacgagta tttcggcaa gtttggcaa gatacataa 
agtcgttat taatcagtc gacggcgta tgcgggctc 
acaggggcc cagcgcaag tgcctacgt gacgtatat 
gaaaagcga tgacgcttt acgcgccat tgaaagtgt 
caataacat aacaatcga cggacgtct cgtaaaatc 
cagtttagg aacgacgaa atattgcag tcactttat 
gaaaaatca accatgtcc aaaaccgga ctgtatgta 
cctccatga tttcggaga tcctgaagc tagttttac 
gaaagagca aatgcacgc cgggaaaca tcaagaata 
cgaaaagaa attacacga agcgctgtt acaacggac 
taataacaa taatacgaa cgtgacaac aacagagac 
aaaaccgac aacaaaaag taaagaacc ggtggcggc 
tgcgacgcc aacgaatgg cacaaataa ggataattg 
gccacagtt gaattccga aaaggagaa tagtaagga 
aaagaaggc ggagaagtc gacggaaag taaagataa 
agttacgaa aggcaaaag taaagaaaa atcgaaaga 
gaagaagga gaagaagcc cgaagttaa agtcgcaaa 
ggataagga agagacgaa gaaagagag tggagagaa 
attatcaat attggacga taacacgag ttttttttc 
gcacaattc gttccaaaa aatcgtccc cgaaaatcg 
agaagacga cgacagtcc caatcaaac atcaccgtc 
tcttctaac cgattctct ccccgacat aaacacgag 
cgaagattg ggccgcagc tttcggttt taataaaac 
aagcgagac aacagttcg gtccgaatc gagtcaatc 
gccaatcga ttcgttttt ccgcaccga cgattttcg 
taatattat cgaagcctc acttacaac agccaaaaa 
taacggtta taccaaacc accacaacc agccaccac 
aacaacaac aacatcgga gaattattt cggtaaaga 
gtcgggttt ggatcaaca catgtccaa gttttttat 
ggattttca tacgaaaaa tggccagaa agagagtaa 
cggttttac gatgaatca taatatgaa cgggtttca 
agtgaataa ttattttat caatcagga tcgtttgtt 
cttgttgca acagaaaaa aatcgagga acatttagt 
gaatctctc gttgaagca acagaatca gagttatgg 
cgggtttat gaatggtga tgtagtggc acagaatgg 
ctatggggg agggtttca aggacagat gtatcagaa 
tccacaacc agtgcaaaa gagtcggac acatgcgga 
ggacgattt gggctttga tcccttcca ggagacgca 
gaaggcctt ggctgagct tatagcgaa cgaacagag 
ttataaggc acatagtaa tgttagagg aggtatgtc 
gtcgagtca aatgaacgg tgtggtcca cagtcagag 
cggtaaaag tcacttacc cccgccccc gcccggttt 
tgtacaacc gacaacaca tatgaattc attcggtag 
taaaatttt accattttt aaatatgtc aaatcaaca 
aattagttc atcaacaca acaacaaca acaaaactg 
gcctagtta taattcgca agtgcagcc gccaccgcc 
accacaaca acagcagaa aaatgcaaa cagctgtaa 
cgattggaa ggatttcga ttcggcaat agtcacaac 
cagtcgtca ctacccaat aatgggacc gataaataa 
tcctttcag atcgttaca acaacagtt aggtacaca 
acaaatacc gaacggatc gttacgatc gcacgaatt 
tacgaataa cgcattttc aaatttcac acaacagtt 
gcaacaaca acagccgag ttattcaaa ttattcatc 
acatttaaa taatacaac acagcaaca acaacagaa 
tctgagttg gtttactag cgaattaaa tacacaaat 
atcaagtcc acccggatt tcgaactgc gactcaaac 
gtctaaaca acaagaatg ttaaaaaac acgcaacgt 
ctaaggtgt ctggatccg ttaaatata taatttttt 
tctttggat ttcattttt ctttcattt tctgattta 
ttacatacg tgatgagcg tttccacaa ttgtgcaat 
aatacagat ggaaaaagc cgactggat agaattgtt 
tcgttgtta ttatttagc aggatccat gcagttaag 
ttattctaa ttatcaaag aaagcgtaa aaagagtac 
taaaatgtg ctttatttt gttataaaa ttctgtggg 
tgcttggcc aacaatctt caaatgatt aaaaactaa 
tattatcct tatattcat ttgattata tgaaataaa 
acagctaaa tatat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY
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