General information | |||
TC033413
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007421.3: 3838544..3844972 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
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TC033413 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_02500 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)vitellogenic egg chamber number decreased - Number of animals showing the phenotype: 4
previtellogenic egg chamber pattern irregular - Number of animals showing the phenotype: 4
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 11.11% (includes death as larva, prepupa, pupa, adult)
egg chamber mostly not present - Number of animals showing the phenotype: 4
tropharium size decreased - Number of animals showing the phenotype: 4
fertility after larval injection decreased (no living offspring) -
- >TC033413 RA
MSVLNQSGE EQVECPLCM EPLEVDDLN FFPCTCGYQ ICRFCWHRI RTDENGLCP ACRKAYSED PADFIPLSQ EQVAKLKAE KRQRDQQRK AKLSESRKH LASVRVVQK NLVFVVGLP MRLAEPEVL KRHEYFGKF GKIHKVVIN QSTAYAGSQ GPSASAYVT YMKSDDALR AIESVNNIT IDGRLVKSS LGTTKYCSH FMKNQPCPK PDCMYLHDF GDPEASFTK EQMHAGKHQ EYEKKLHEA LLQRTNNNN TNVTTTETK PTTKSKEPV AAATPTNGT NKDNWPQLN SEKENSKEK KAEKSTESK DKVTKGKSK EKSKEKKEK KPEVKVAKD KEETKKESG EKLSILDDN TSFFSHNSF QKIVPENRE DDDSPNQTS PSLLTDSLP DINTSEDWA AAFGFNKTS ETTVRSESS QSPIDSFFR TDDFRNIIE ASLTTAKNN GYTKPPQPA TTTTTTSEN YFGKESGLD QHMSKFFMD FHTKNGQKE SNGFTMNHN MNGFQVNNY FINQDRLFL LQQKKIEEH LVNLSLKQQ NQSYGGFMN GDVVAQNGY GGGFQGQMY QNPQPVQKS RTHAEDDLG FDPFQETQK ALAELIANE QSYKAHSNV RGGMSSSQM NGVVHSQSG KSHLPPPPP GFVQPTTHM NSFGSKILP FLNMSNQQI SSSTQQQQQ NWPSYNSQV QPPPPPQQQ QKNANSCND WKDFDSAIV TTSRHYPIM GPINNPFRS LQQQLGTQQ IPNGSLRSH EFTNNAFSN FTQQLQQQQ PSYSNYSSH LNNTTQQQQ QNLSWFTSE LNTQISSPP GFRTATQTS KQQEC
- >TC033413 RA
atgtcagtg ttgaatcaa agcggggaa gagcaagtc gagtgcccc ctgtgcatg gagcccctc gaggtggac gatttgaac tttttcccg tgcacttgc ggctatcag atttgtcgg ttctgctgg cacagaatc cggaccgat gaaaacggt ctgtgtcca gcgtgccgc aaggcctac tcggaggac cccgccgac ttcatcccc ctgtctcag gagcaagtg gcgaagctc aaggccgag aagagacag agggatcag cagaggaag gcgaagctg agcgagagc aggaaacat ctagcgagt gttagggtc gtgcaaaag aatctggtg tttgtggtg gggctgccg atgagatta gccgagccc gaggtgctc aaaaggcac gagtatttc ggcaagttt ggcaagata cataaagtc gttattaat cagtcgacg gcgtatgcg ggctcacag gggcccagc gcaagtgcc tacgtgacg tatatgaaa agcgatgac gctttacgc gccattgaa agtgtcaat aacataaca atcgacgga cgtctcgta aaatccagt ttaggaacg acgaaatat tgcagtcac tttatgaaa aatcaacca tgtccaaaa ccggactgt atgtacctc catgatttc ggagatcct gaagctagt tttacgaaa gagcaaatg cacgccggg aaacatcaa gaatacgaa aagaaatta cacgaagcg ctgttacaa cggactaat aacaataat acgaacgtg acaacaaca gagacaaaa ccgacaaca aaaagtaaa gaaccggtg gcggctgcg acgccaacg aatggcaca aataaggat aattggcca cagttgaat tccgaaaag gagaatagt aaggaaaag aaggcggag aagtcgacg gaaagtaaa gataaagtt acgaaaggc aaaagtaaa gaaaaatcg aaagagaag aaggagaag aagcccgaa gttaaagtc gcaaaggat aaggaagag acgaagaaa gagagtgga gagaaatta tcaatattg gacgataac acgagtttt ttttcgcac aattcgttc caaaaaatc gtccccgaa aatcgagaa gacgacgac agtcccaat caaacatca ccgtctctt ctaaccgat tctctcccc gacataaac acgagcgaa gattgggcc gcagctttc ggttttaat aaaacaagc gagacaaca gttcggtcc gaatcgagt caatcgcca atcgattcg tttttccgc accgacgat tttcgtaat attatcgaa gcctcactt acaacagcc aaaaataac ggttatacc aaaccacca caaccagcc accacaaca acaacaaca tcggagaat tatttcggt aaagagtcg ggtttggat caacacatg tccaagttt tttatggat tttcatacg aaaaatggc cagaaagag agtaacggt tttacgatg aatcataat atgaacggg tttcaagtg aataattat tttatcaat caggatcgt ttgttcttg ttgcaacag aaaaaaatc gaggaacat ttagtgaat ctctcgttg aagcaacag aatcagagt tatggcggg tttatgaat ggtgatgta gtggcacag aatggctat gggggaggg tttcaagga cagatgtat cagaatcca caaccagtg caaaagagt cggacacat gcggaggac gatttgggc tttgatccc ttccaggag acgcagaag gccttggct gagcttata gcgaacgaa cagagttat aaggcacat agtaatgtt agaggaggt atgtcgtcg agtcaaatg aacggtgtg gtccacagt cagagcggt aaaagtcac ttacccccg cccccgccc ggttttgta caaccgaca acacatatg aattcattc ggtagtaaa attttacca tttttaaat atgtcaaat caacaaatt agttcatca acacaacaa caacaacaa aactggcct agttataat tcgcaagtg cagccgcca ccgccacca caacaacag cagaaaaat gcaaacagc tgtaacgat tggaaggat ttcgattcg gcaatagtc acaaccagt cgtcactac ccaataatg ggaccgata aataatcct ttcagatcg ttacaacaa cagttaggt acacaacaa ataccgaac ggatcgtta cgatcgcac gaatttacg aataacgca ttttcaaat ttcacacaa cagttgcaa caacaacag ccgagttat tcaaattat tcatcacat ttaaataat acaacacag caacaacaa cagaatctg agttggttt actagcgaa ttaaataca caaatatca agtccaccc ggatttcga actgcgact caaacgtct aaacaacaa gaatgttaa
- >TC033413 RA
acattttgt aaacaaagg tttcatccc tgacaagtg taaacgtgg ctttagtag gtgaatgtg tttggagcg catgcgcaa gcctacgag atcatagtc gccatattt ccaatctgt ctaatctga aaattcagt aagtaaatt acgttttcg cacggattc gcgtgtttt tgtccgaaa tcgccgccg tttctctct caattcccc cgaaattta gcgtgatcg cgcaatgtc agtgttgaa tcaaagcgg ggaagagca agtcgagtg ccccctgtg catggagcc cctcgaggt ggacgattt gaacttttt cccgtgcac ttgcggcta tcagatttg tcggttctg ctggcacag aatccggac cgatgaaaa cggtctgtg tccagcgtg ccgcaaggc ctactcgga ggaccccgc cgacttcat ccccctgtc tcaggagca agtggcgaa gctcaaggc cgagaagag acagaggga tcagcagag gaaggcgaa gctgagcga gagcaggaa acatctagc gagtgttag ggtcgtgca aaagaatct ggtgtttgt ggtggggct gccgatgag attagccga gcccgaggt gctcaaaag gcacgagta tttcggcaa gtttggcaa gatacataa agtcgttat taatcagtc gacggcgta tgcgggctc acaggggcc cagcgcaag tgcctacgt gacgtatat gaaaagcga tgacgcttt acgcgccat tgaaagtgt caataacat aacaatcga cggacgtct cgtaaaatc cagtttagg aacgacgaa atattgcag tcactttat gaaaaatca accatgtcc aaaaccgga ctgtatgta cctccatga tttcggaga tcctgaagc tagttttac gaaagagca aatgcacgc cgggaaaca tcaagaata cgaaaagaa attacacga agcgctgtt acaacggac taataacaa taatacgaa cgtgacaac aacagagac aaaaccgac aacaaaaag taaagaacc ggtggcggc tgcgacgcc aacgaatgg cacaaataa ggataattg gccacagtt gaattccga aaaggagaa tagtaagga aaagaaggc ggagaagtc gacggaaag taaagataa agttacgaa aggcaaaag taaagaaaa atcgaaaga gaagaagga gaagaagcc cgaagttaa agtcgcaaa ggataagga agagacgaa gaaagagag tggagagaa attatcaat attggacga taacacgag ttttttttc gcacaattc gttccaaaa aatcgtccc cgaaaatcg agaagacga cgacagtcc caatcaaac atcaccgtc tcttctaac cgattctct ccccgacat aaacacgag cgaagattg ggccgcagc tttcggttt taataaaac aagcgagac aacagttcg gtccgaatc gagtcaatc gccaatcga ttcgttttt ccgcaccga cgattttcg taatattat cgaagcctc acttacaac agccaaaaa taacggtta taccaaacc accacaacc agccaccac aacaacaac aacatcgga gaattattt cggtaaaga gtcgggttt ggatcaaca catgtccaa gttttttat ggattttca tacgaaaaa tggccagaa agagagtaa cggttttac gatgaatca taatatgaa cgggtttca agtgaataa ttattttat caatcagga tcgtttgtt cttgttgca acagaaaaa aatcgagga acatttagt gaatctctc gttgaagca acagaatca gagttatgg cgggtttat gaatggtga tgtagtggc acagaatgg ctatggggg agggtttca aggacagat gtatcagaa tccacaacc agtgcaaaa gagtcggac acatgcgga ggacgattt gggctttga tcccttcca ggagacgca gaaggcctt ggctgagct tatagcgaa cgaacagag ttataaggc acatagtaa tgttagagg aggtatgtc gtcgagtca aatgaacgg tgtggtcca cagtcagag cggtaaaag tcacttacc cccgccccc gcccggttt tgtacaacc gacaacaca tatgaattc attcggtag taaaatttt accattttt aaatatgtc aaatcaaca aattagttc atcaacaca acaacaaca acaaaactg gcctagtta taattcgca agtgcagcc gccaccgcc accacaaca acagcagaa aaatgcaaa cagctgtaa cgattggaa ggatttcga ttcggcaat agtcacaac cagtcgtca ctacccaat aatgggacc gataaataa tcctttcag atcgttaca acaacagtt aggtacaca acaaatacc gaacggatc gttacgatc gcacgaatt tacgaataa cgcattttc aaatttcac acaacagtt gcaacaaca acagccgag ttattcaaa ttattcatc acatttaaa taatacaac acagcaaca acaacagaa tctgagttg gtttactag cgaattaaa tacacaaat atcaagtcc acccggatt tcgaactgc gactcaaac gtctaaaca acaagaatg ttaaaaaac acgcaacgt ctaaggtgt ctggatccg ttaaatata taatttttt tctttggat ttcattttt ctttcattt tctgattta ttacatacg tgatgagcg tttccacaa ttgtgcaat aatacagat ggaaaaagc cgactggat agaattgtt tcgttgtta ttatttagc aggatccat gcagttaag ttattctaa ttatcaaag aaagcgtaa aaagagtac taaaatgtg ctttatttt gttataaaa ttctgtggg tgcttggcc aacaatctt caaatgatt aaaaactaa tattatcct tatattcat ttgattata tgaaataaa acagctaaa tatat
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
