Status
Please wait ...
Gene information
General information
TC015632 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 3754236..3757983
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015632
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02499

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 44.44% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015632 RA
      MFPELPPIY WCLALTRII LVLIPQLGY IHPDEFFQS 
      IEVQAGKAF HVESNIPWE YNATFPVRS VTIPYFTIG 
      MVYKLANVA EYFLKQYST LSIITPYLV VVGPRFLMC 
      ALSFLVDLI LYKICVNNN EKYKNRLVV LASSYVVLV 
      YGTRTFSNT FELVLFALL QYYVCESLV FSNTLIKKR 
      EYLNYRYER SRTVVEKAK FHKLRLLLT SDSLRNCVQ 
      ISVITVLGF FNRPTFLAF AMFPVFFWL YRGLGNKLV 
      APMNYHLRI LALIVCSIP TILLSVLID SFYYGYITW 
      GEVGVLHVT IDNFVFTPL NFILYNMDS GNLAEHGLH 
      PRFLHVLVN MPLLFGLLA LFAYYNFCQ AVYNLVTGR 
      HHLLPTVRS IKGLMMLSY LGPLTILSI FPHQEPRFL 
      IPLILPLVY LSTNSVFPE SDNILIKLE ASTLKNRAP 
      EMAKSPTTF LKLWLVVNL ILVLFFGFL HQGGVYRSG 
      EFLYKDMKR HSRTDFYVV TSHIYSMPK SLVLQKSSD 
      VLHHTKRTK YTVNKRFFL YEKGSSDLD LVVKDLQIL 
      RGKLQDYRW NFKSKKYKL ILIIPSSLE DRLSYSANS 
      QFEFKLVTT FYPHISTEA FPDLGQDLF YFYSNLGEQ 
      DFYRSVLRC LIEFFHSFG LNLYEIEV
    CDS sequence
    • >TC015632 RA
      atgtttcct gaacttccc ccgatttac tggtgcctg 
      gcactgacc cgaatcatc ctagtccta ataccacaa 
      ctaggctac atccaccca gacgaattc ttccagtca 
      attgaagtg caagcaggc aaagctttc cacgtcgaa 
      agcaacatc ccgtgggaa tacaacgcc acattccca 
      gtccggagc gtaacaatc ccatatttt accatcggc 
      atggtttac aagctggcc aatgtggct gaatatttc 
      ctcaaacaa tactcaaca ctttcgata attacgccc 
      tacttggtg gttgtaggc cctcgtttt ttaatgtgt 
      gcgttatca ttccttgtc gatttaatt ctctacaaa 
      atctgcgtc aacaacaac gaaaagtac aaaaaccgg 
      cttgtagtc ctggccagt tcttacgtg gtcttagtt 
      tacggcacg cgcactttt tcgaacact ttcgagctc 
      gtacttttc gcgctcctc caatactac gtctgcgag 
      agtttagtt ttttcaaat actctgatt aaaaagcgc 
      gaatattta aattatcga tacgagagg agtcgaaca 
      gttgtagaa aaagcaaag tttcataaa ttgcgactg 
      ttgctaacg tctgattcc ttgcggaat tgtgtgcag 
      attagcgtg atcacggtt ttgggtttt ttcaatcgg 
      cccactttt ctcgcattt gcaatgttt ccggtgttt 
      ttctggctg tatagaggg ttgggtaat aaattagtc 
      gcgcctatg aattaccat ttgaggata ttggcactg 
      attgtgtgc tcaattcct acgattctg ctcagtgtt 
      ttaatcgat tcgttctac tacgggtat ataacgtgg 
      ggagaggtc ggggtttta cacgttacg atcgataat 
      tttgtcttc acgcctttg aactttatt ttgtataat 
      atggattcg ggtaatttg gctgagcac gggctacat 
      ccgagattt ttacacgtg cttgtaaac atgccttta 
      ctctttggt ctgctcgcc ttgttcgcc tactacaat 
      ttttgccaa gcggtgtac aatttggta accggtcga 
      catcaccta ctaccaact gtacgaagt atcaagggc 
      cttatgatg ttatcatac ttgggcccc ctgacaatc 
      ctgtcaata ttccctcac caggagccc cgtttccta 
      atcccgttg atcctccca ctggtgtat ttatccacc 
      aatagcgta ttccccgaa tctgacaat attttgatc 
      aaactcgaa gcctcaacg ttaaaaaac cgtgccccc 
      gaaatggcc aaaagccca acaactttc ctcaaactg 
      tggctagtt gtgaattta attctggtg ctatttttc 
      ggcttcctg caccaaggc ggagtgtac cgatctggt 
      gaatttctg tacaaagac atgaaacgg cactcgaga 
      actgacttt tacgttgtc accagtcat atttactca 
      atgcccaaa agtctagtt ttgcaaaaa tcgagcgat 
      gttctgcac cacaccaag cgcacgaaa tacaccgtg 
      aataagcgg ttttttctc tatgagaaa gggtcgtcc 
      gacttggac ttggtcgta aaagacttg caaatattg 
      cgtgggaag ctccaagat tatagatgg aatttcaag 
      agtaaaaag tataaattg attttgatt attccaagt 
      agtttggag gaccgcttg agttattca gcgaatagt 
      cagttcgaa tttaaactc gtcacaact ttttatcca 
      catatcagt accgaggcg tttccggat ttgggccaa 
      gatttgttt tatttctac tcgaatttg ggggagcaa 
      gatttttat aggtcggta ttgcggtgc ttgattgag 
      ttttttcac tcgtttggg ctcaatttg tacgaaatt 
      gaagtgtag
    mRNA sequence
    • >TC015632 RA
      tttgtcaga atatttgtc aaaattcaa aatgtaaac 
      aaaatgttt cctgaactt cccccgatt tactggtgc 
      ctggcactg acccgaatc atcctagtc ctaatacca 
      caactaggc tacatccac ccagacgaa ttcttccag 
      tcaattgaa gtgcaagca ggcaaagct ttccacgtc 
      gaaagcaac atcccgtgg gaatacaac gccacattc 
      ccagtccgg agcgtaaca atcccatat tttaccatc 
      ggcatggtt tacaagctg gccaatgtg gctgaatat 
      ttcctcaaa caatactca acactttcg ataattacg 
      ccctacttg gtggttgta ggccctcgt tttttaatg 
      tgtgcgtta tcattcctt gtcgattta attctctac 
      aaaatctgc gtcaacaac aacgaaaag tacaaaaac 
      cggcttgta gtcctggcc agttcttac gtggtctta 
      gtttacggc acgcgcact ttttcgaac actttcgag 
      ctcgtactt ttcgcgctc ctccaatac tacgtctgc 
      gagagttta gttttttca aatactctg attaaaaag 
      cgcgaatat ttaaattat cgatacgag aggagtcga 
      acagttgta gaaaaagca aagtttcat aaattgcga 
      ctgttgcta acgtctgat tccttgcgg aattgtgtg 
      cagattagc gtgatcacg gttttgggt tttttcaat 
      cggcccact tttctcgca tttgcaatg tttccggtg 
      tttttctgg ctgtataga gggttgggt aataaatta 
      gtcgcgcct atgaattac catttgagg atattggca 
      ctgattgtg tgctcaatt cctacgatt ctgctcagt 
      gttttaatc gattcgttc tactacggg tatataacg 
      tggggagag gtcggggtt ttacacgtt acgatcgat 
      aattttgtc ttcacgcct ttgaacttt attttgtat 
      aatatggat tcgggtaat ttggctgag cacgggcta 
      catccgaga tttttacac gtgcttgta aacatgcct 
      ttactcttt ggtctgctc gccttgttc gcctactac 
      aatttttgc caagcggtg tacaatttg gtaaccggt 
      cgacatcac ctactacca actgtacga agtatcaag 
      ggccttatg atgttatca tacttgggc cccctgaca 
      atcctgtca atattccct caccaggag ccccgtttc 
      ctaatcccg ttgatcctc ccactggtg tatttatcc 
      accaatagc gtattcccc gaatctgac aatattttg 
      atcaaactc gaagcctca acgttaaaa aaccgtgcc 
      cccgaaatg gccaaaagc ccaacaact ttcctcaaa 
      ctgtggcta gttgtgaat ttaattctg gtgctattt 
      ttcggcttc ctgcaccaa ggcggagtg taccgatct 
      ggtgaattt ctgtacaaa gacatgaaa cggcactcg 
      agaactgac ttttacgtt gtcaccagt catatttac 
      tcaatgccc aaaagtcta gttttgcaa aaatcgagc 
      gatgttctg caccacacc aagcgcacg aaatacacc 
      gtgaataag cggtttttt ctctatgag aaagggtcg 
      tccgacttg gacttggtc gtaaaagac ttgcaaata 
      ttgcgtggg aagctccaa gattataga tggaatttc 
      aagagtaaa aagtataaa ttgattttg attattcca 
      agtagtttg gaggaccgc ttgagttat tcagcgaat 
      agtcagttc gaatttaaa ctcgtcaca actttttat 
      ccacatatc agtaccgag gcgtttccg gatttgggc 
      caagatttg ttttatttc tactcgaat ttgggggag 
      caagatttt tataggtcg gtattgcgg tgcttgatt 
      gagtttttt cactcgttt gggctcaat ttgtacgaa 
      attgaagtg tagagggag agaaggcgt tggttctgc 
      tgatttgat tgaaatttt gctgtttta atcgcctgt 
      taattactc gtagaagtt ttactattt gtatacagt 
      gtgctatga tctgtttat taaattata ttgaaatga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY