General information | |||
TC015632 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007421.3: 3754236..3757983 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC015632 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_02499 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 44.44% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 1 (16 dpi)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (16 dpi)
- >TC015632 RA
MFPELPPIY WCLALTRII LVLIPQLGY IHPDEFFQS IEVQAGKAF HVESNIPWE YNATFPVRS VTIPYFTIG MVYKLANVA EYFLKQYST LSIITPYLV VVGPRFLMC ALSFLVDLI LYKICVNNN EKYKNRLVV LASSYVVLV YGTRTFSNT FELVLFALL QYYVCESLV FSNTLIKKR EYLNYRYER SRTVVEKAK FHKLRLLLT SDSLRNCVQ ISVITVLGF FNRPTFLAF AMFPVFFWL YRGLGNKLV APMNYHLRI LALIVCSIP TILLSVLID SFYYGYITW GEVGVLHVT IDNFVFTPL NFILYNMDS GNLAEHGLH PRFLHVLVN MPLLFGLLA LFAYYNFCQ AVYNLVTGR HHLLPTVRS IKGLMMLSY LGPLTILSI FPHQEPRFL IPLILPLVY LSTNSVFPE SDNILIKLE ASTLKNRAP EMAKSPTTF LKLWLVVNL ILVLFFGFL HQGGVYRSG EFLYKDMKR HSRTDFYVV TSHIYSMPK SLVLQKSSD VLHHTKRTK YTVNKRFFL YEKGSSDLD LVVKDLQIL RGKLQDYRW NFKSKKYKL ILIIPSSLE DRLSYSANS QFEFKLVTT FYPHISTEA FPDLGQDLF YFYSNLGEQ DFYRSVLRC LIEFFHSFG LNLYEIEV
- >TC015632 RA
atgtttcct gaacttccc ccgatttac tggtgcctg gcactgacc cgaatcatc ctagtccta ataccacaa ctaggctac atccaccca gacgaattc ttccagtca attgaagtg caagcaggc aaagctttc cacgtcgaa agcaacatc ccgtgggaa tacaacgcc acattccca gtccggagc gtaacaatc ccatatttt accatcggc atggtttac aagctggcc aatgtggct gaatatttc ctcaaacaa tactcaaca ctttcgata attacgccc tacttggtg gttgtaggc cctcgtttt ttaatgtgt gcgttatca ttccttgtc gatttaatt ctctacaaa atctgcgtc aacaacaac gaaaagtac aaaaaccgg cttgtagtc ctggccagt tcttacgtg gtcttagtt tacggcacg cgcactttt tcgaacact ttcgagctc gtacttttc gcgctcctc caatactac gtctgcgag agtttagtt ttttcaaat actctgatt aaaaagcgc gaatattta aattatcga tacgagagg agtcgaaca gttgtagaa aaagcaaag tttcataaa ttgcgactg ttgctaacg tctgattcc ttgcggaat tgtgtgcag attagcgtg atcacggtt ttgggtttt ttcaatcgg cccactttt ctcgcattt gcaatgttt ccggtgttt ttctggctg tatagaggg ttgggtaat aaattagtc gcgcctatg aattaccat ttgaggata ttggcactg attgtgtgc tcaattcct acgattctg ctcagtgtt ttaatcgat tcgttctac tacgggtat ataacgtgg ggagaggtc ggggtttta cacgttacg atcgataat tttgtcttc acgcctttg aactttatt ttgtataat atggattcg ggtaatttg gctgagcac gggctacat ccgagattt ttacacgtg cttgtaaac atgccttta ctctttggt ctgctcgcc ttgttcgcc tactacaat ttttgccaa gcggtgtac aatttggta accggtcga catcaccta ctaccaact gtacgaagt atcaagggc cttatgatg ttatcatac ttgggcccc ctgacaatc ctgtcaata ttccctcac caggagccc cgtttccta atcccgttg atcctccca ctggtgtat ttatccacc aatagcgta ttccccgaa tctgacaat attttgatc aaactcgaa gcctcaacg ttaaaaaac cgtgccccc gaaatggcc aaaagccca acaactttc ctcaaactg tggctagtt gtgaattta attctggtg ctatttttc ggcttcctg caccaaggc ggagtgtac cgatctggt gaatttctg tacaaagac atgaaacgg cactcgaga actgacttt tacgttgtc accagtcat atttactca atgcccaaa agtctagtt ttgcaaaaa tcgagcgat gttctgcac cacaccaag cgcacgaaa tacaccgtg aataagcgg ttttttctc tatgagaaa gggtcgtcc gacttggac ttggtcgta aaagacttg caaatattg cgtgggaag ctccaagat tatagatgg aatttcaag agtaaaaag tataaattg attttgatt attccaagt agtttggag gaccgcttg agttattca gcgaatagt cagttcgaa tttaaactc gtcacaact ttttatcca catatcagt accgaggcg tttccggat ttgggccaa gatttgttt tatttctac tcgaatttg ggggagcaa gatttttat aggtcggta ttgcggtgc ttgattgag ttttttcac tcgtttggg ctcaatttg tacgaaatt gaagtgtag
- >TC015632 RA
tttgtcaga atatttgtc aaaattcaa aatgtaaac aaaatgttt cctgaactt cccccgatt tactggtgc ctggcactg acccgaatc atcctagtc ctaatacca caactaggc tacatccac ccagacgaa ttcttccag tcaattgaa gtgcaagca ggcaaagct ttccacgtc gaaagcaac atcccgtgg gaatacaac gccacattc ccagtccgg agcgtaaca atcccatat tttaccatc ggcatggtt tacaagctg gccaatgtg gctgaatat ttcctcaaa caatactca acactttcg ataattacg ccctacttg gtggttgta ggccctcgt tttttaatg tgtgcgtta tcattcctt gtcgattta attctctac aaaatctgc gtcaacaac aacgaaaag tacaaaaac cggcttgta gtcctggcc agttcttac gtggtctta gtttacggc acgcgcact ttttcgaac actttcgag ctcgtactt ttcgcgctc ctccaatac tacgtctgc gagagttta gttttttca aatactctg attaaaaag cgcgaatat ttaaattat cgatacgag aggagtcga acagttgta gaaaaagca aagtttcat aaattgcga ctgttgcta acgtctgat tccttgcgg aattgtgtg cagattagc gtgatcacg gttttgggt tttttcaat cggcccact tttctcgca tttgcaatg tttccggtg tttttctgg ctgtataga gggttgggt aataaatta gtcgcgcct atgaattac catttgagg atattggca ctgattgtg tgctcaatt cctacgatt ctgctcagt gttttaatc gattcgttc tactacggg tatataacg tggggagag gtcggggtt ttacacgtt acgatcgat aattttgtc ttcacgcct ttgaacttt attttgtat aatatggat tcgggtaat ttggctgag cacgggcta catccgaga tttttacac gtgcttgta aacatgcct ttactcttt ggtctgctc gccttgttc gcctactac aatttttgc caagcggtg tacaatttg gtaaccggt cgacatcac ctactacca actgtacga agtatcaag ggccttatg atgttatca tacttgggc cccctgaca atcctgtca atattccct caccaggag ccccgtttc ctaatcccg ttgatcctc ccactggtg tatttatcc accaatagc gtattcccc gaatctgac aatattttg atcaaactc gaagcctca acgttaaaa aaccgtgcc cccgaaatg gccaaaagc ccaacaact ttcctcaaa ctgtggcta gttgtgaat ttaattctg gtgctattt ttcggcttc ctgcaccaa ggcggagtg taccgatct ggtgaattt ctgtacaaa gacatgaaa cggcactcg agaactgac ttttacgtt gtcaccagt catatttac tcaatgccc aaaagtcta gttttgcaa aaatcgagc gatgttctg caccacacc aagcgcacg aaatacacc gtgaataag cggtttttt ctctatgag aaagggtcg tccgacttg gacttggtc gtaaaagac ttgcaaata ttgcgtggg aagctccaa gattataga tggaatttc aagagtaaa aagtataaa ttgattttg attattcca agtagtttg gaggaccgc ttgagttat tcagcgaat agtcagttc gaatttaaa ctcgtcaca actttttat ccacatatc agtaccgag gcgtttccg gatttgggc caagatttg ttttatttc tactcgaat ttgggggag caagatttt tataggtcg gtattgcgg tgcttgatt gagtttttt cactcgttt gggctcaat ttgtacgaa attgaagtg tagagggag agaaggcgt tggttctgc tgatttgat tgaaatttt gctgtttta atcgcctgt taattactc gtagaagtt ttactattt gtatacagt gtgctatga tctgtttat taaattata ttgaaatga
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
