Status
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Gene information
General information
TC015622 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 3532566..3574532
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC015622
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02498

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 3
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 7 (16 dpi)
    Days post injection


    Pupal morphology
    head slightly orientation irregular - Number of animals showing the phenotype: 9

    wings dv-orientation irregular - Number of animals showing the phenotype: 9

    wings potentially shortened - Number of animals showing the phenotype: 9
    Adult morphology
    flagellomeres shape irregular - Number of animals showing the phenotype: 7


    flagellomeres slightlynumber decreased - Number of animals showing the phenotype: 7

    elytra blistered - Number of animals showing the phenotype: 7


    iBeetle screen iB_06352

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Fertility
    egg chamber mostly not present - Number of animals showing the phenotype: 3

    egg chamber number decreased - Number of animals showing the phenotype: 5
    tropharium sometimes shape irregular - Number of animals showing the phenotype: 5
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 9 (16 dpi)
    Days post injection


    Adult morphology
    flagellomeres number decreased - Number of animals showing the phenotype: 9


    funicle shortened - Number of animals showing the phenotype: 9

    elytra dorsal not closed - Number of animals showing the phenotype: 9

    legs tarsomere sometimes shortened - Number of animals showing the phenotype: 9


    legs tarsomere sometimes number decreased - Number of animals showing the phenotype: 3


    legs femur broader - Number of animals showing the phenotype: 9


    legs tibia slightly broader - Number of animals showing the phenotype: 9


    elytra blistered - Number of animals showing the phenotype: 2

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015622 RA
      MFWFAICAL LLTPIASST EPFDEYTAP KLDVDNLEN 
GYHGLVKEN ETLVEVTPA IRAIGEQVC SFRIVNKHH 
GEAPFEIVS KEDGYAELR ARRVLNCEK RRNYKFDIA 
AVGCNGKQS VSATVHITV SDVNEYAPT FSEPSYVRQ 
VDEGRIYSE IVRVEATDR DCTPKFGDV CKYEILTSD 
QPFVIDMDG VIRNTEPLD YERSHNHIL SVVAYDCGM 
RQSLPVMVT IKVNRVCRL GWRGLPERV DYAPTTGKQ 
ELFPSASLE LCDVPCNVR ELRTRVDLA TRHIGKGCD 
RDTYSVQSQ RKLCGASPS AIDLLPSPG VGAEWTKPL 
ISDEGHESD QMFEFDGSS TAVSIPNSV LDHNLPSTF 
TIATWMRHG QHNGQDKRV KEHILCSAD DHKMNRHHY 
ALFVRNCRL ILLLRRDFS EGDLNIFRP AEWRWKIPQ 
VCDDQWHHY AVNVRFPDI ELYVDGQLF RSEKKNPEV 
IDDWPLHPT KGINTTLTV GACWQGSDN KMKHHLKGY 
LAGLSVLVG ETEKPEVLS CLHQCKESL QVPAMELLQ 
PGMELLTNS DLTEVTVEG DNRTNLETL VRKIGYANS 
REFPTPGRR NLRLTTTVT CDNGKTVKV PDAQTYVMV 
LRPQTPSIN LNGTGNMAR KYEDFRMGV RVLADIHIS 
GEQKLDKCS LTIYPNLNP DHENLSVPE EMLKRLGMA 
ARVSKDGVT ITGSDMVFN YQQVLRQIV YTNRKPAYY 
LNRVFKLTC SELNGRFTS NEYVQTLTV IHPQPKEPI 
VAHATVNKH NVELRPASV VSRDYIPSE HLNLVASGG 
SHAVTIIVV VCVGFLLFM IVLGVIRIR AAHHRNASE 
EAQDSEMTW DDSALTITV NPMENAERK LEGHSADYS 
ESDQSDSDS SSDDDNIDG PCRIPDSSD DEEEQPARS 
RKHDYQNIS QLEWDHSTM
    CDS sequence
    • >TC015622 RA
      atgttttgg ttcgcgatc tgtgccctc cttctgact 
cccatcgct tcttcgacg gagccgttc gacgagtac 
acagctccc aaattggac gtggacaat ttggagaac 
ggctaccac ggcctcgtg aaagagaac gagacgttg 
gtcgaagtg acgccggcg atacgcgcc atcggcgaa 
caagtttgc agttttcgc atcgtaaac aagcaccat 
ggcgaagct ccatttgag atcgtctcg aaagaggac 
ggctatgcc gaactcagg gccagaagg gtgctcaac 
tgcgagaaa agaaggaac tacaagttc gacattgct 
gccgtcggc tgtaacggc aagcagagc gtcagcgcc 
accgttcac ataaccgtc tcagatgtg aacgaatac 
gcaccgact ttctccgaa ccaagctac gtaaggcaa 
gtcgatgaa ggacgaatc tacagcgag attgttcgt 
gttgaagcg accgatcgt gattgtacg ccgaagttc 
ggcgatgtc tgcaaatat gaaatactt acgtcagat 
cagcctttc gtcatcgac atggatggg gttattagg 
aatacggag cctttggat tatgagagg tcgcataac 
cacatactc agtgttgtg gcttacgac tgtggaatg 
cgtcaaagc ttgcccgtc atggtcacc atcaaagtc 
aaccgtgtc tgtcgactc ggctggcga ggacttccg 
gaaagagtc gattatgct ccgaccacc ggaaaacag 
gaactattc ccttcagcc agtttggaa ttgtgcgac 
gttccatgt aacgtgagg gaattacga acgagggtt 
gatttagcc accagacat atcggcaaa ggttgcgat 
cgtgacaca tacagcgtc cagtctcag cgcaaatta 
tgcggggct tccccgtcg gctatcgat ctacttccg 
agtccgggt gtcggtgcc gaatggacg aagccgctg 
atttccgac gagggacac gaatcggat cagatgttt 
gaatttgac ggctccagc actgccgtt tccattccg 
aattcggtc ttggatcat aatttgccg tcaacgttc 
acgatagcc acgtggatg agacatgga caacataat 
gggcaggat aaacgcgtc aaggaacat attttgtgt 
tcggctgac gaccataaa atgaaccgc caccactac 
gccctcttt gtccgcaac tgccgcctc atcctcctc 
ctccggcgc gacttctcc gaaggcgac ctcaacatc 
ttccgtccg gcggaatgg cgttggaag atcccccag 
gtctgcgac gaccaatgg catcattac gccgtcaac 
gtgcgtttc cccgacatt gaactctac gtcgacggt 
caactcttc cgttccgag aagaaaaac ccggaagtc 
atcgacgat tggccactc catcccacg aaaggcatc 
aacaccacg ttgacagtc ggcgcctgc tggcagggc 
tccgacaac aagatgaag caccatctc aagggctat 
ctggccggc ttgagcgtc ctcgttggc gagacggaa 
aagcccgaa gtgttgagc tgtctgcat cagtgcaaa 
gagagtctt caagtgccc gcaatggaa ctccttcaa 
cctggaatg gaactgttg acgaattcg gatctgacg 
gaagttaca gtcgaggga gataatcgc acgaatttg 
gaaactttg gttcggaag atcgggtat gcgaatagt 
cgggagttt ccgacgccg ggaaggagg aatttgagg 
ctgacgacg acggttacg tgcgataac gggaagacg 
gtcaaggtg cccgatgcg caaacttat gtcatggtt 
ttaaggccg caaacgccc tcgatcaat ctcaatggg 
acggggaac atggcgagg aaatacgag gatttcagg 
atgggggtt cgcgtcctg gccgacatc cacatatcc 
ggggaacaa aagctcgac aaatgctcg ttaaccatc 
taccctaac ttaaacccc gaccatgag aacttaagc 
gttcccgaa gaaatgtta aaaagactc ggaatggca 
gctcgtgta tcaaaagat ggtgttacc atcaccgga 
tccgacatg gtcttcaac taccaacaa gtactcaga 
caaatcgtc tacaccaac cgaaaaccc gcctactac 
ctaaaccga gtgttcaaa ctaacctgc tccgaatta 
aacggacga ttcacgtca aatgaatac gtgcaaact 
ctcactgtg atccatcct cagcccaaa gagcccatc 
gtagctcat gcaactgtg aacaaacac aatgtggaa 
ttacgacca gcgtcggtc gtttcacgt gattacatt 
ccttcggaa catttgaat ttggtcgca agtggggga 
tcacatgct gttacaata atcgtcgta gtgtgcgtc 
ggatttttg ttgtttatg attgtcttg ggtgtgatc 
aggatcagg gcggctcat catagaaat gcatccgag 
gaggcgcaa gacagcgag atgacttgg gatgattcc 
gcgctgaca atcaccgtg aacccaatg gagaacgct 
gaacgcaaa ctggaaggc cactcagcc gactacagc 
gaaagcgac caatccgat tcggacagt tcatcggac 
gacgacaac atcgacggt ccgtgcaga attcccgac 
tcttcggac gacgaagag gagcaaccg gctcgtagt 
cgcaaacac gactatcaa aatatcagc caactagaa 
tgggaccat tctacgatg taa
    mRNA sequence
    • >TC015622 RA
      gggagacag tcaatgaaa acctagccg tccatgacg 
acacaattt agtgagtcg gtatgaaaa tcgtgtgtt 
tatcatcat aaaaaagcg agttgtggt gtgtgcccc 
gtgcgcgta caagacccc catcagacg tttttgtcg 
cctcgccgc cgaaatgtt ttggttcgc gatctgtgc 
cctccttct gactcccat cgcttcttc gacggagcc 
gttcgacga gtacacagc tcccaaatt ggacgtgga 
caatttgga gaacggcta ccacggcct cgtgaaaga 
gaacgagac gttggtcga agtgacgcc ggcgatacg 
cgccatcgg cgaacaagt ttgcagttt tcgcatcgt 
aaacaagca ccatggcga agctccatt tgagatcgt 
ctcgaaaga ggacggcta tgccgaact cagggccag 
aagggtgct caactgcga gaaaagaag gaactacaa 
gttcgacat tgctgccgt cggctgtaa cggcaagca 
gagcgtcag cgccaccgt tcacataac cgtctcaga 
tgtgaacga atacgcacc gactttctc cgaaccaag 
ctacgtaag gcaagtcga tgaaggacg aatctacag 
cgagattgt tcgtgttga agcgaccga tcgtgattg 
tacgccgaa gttcggcga tgtctgcaa atatgaaat 
acttacgtc agatcagcc tttcgtcat cgacatgga 
tggggttat taggaatac ggagccttt ggattatga 
gaggtcgca taaccacat actcagtgt tgtggctta 
cgactgtgg aatgcgtca aagcttgcc cgtcatggt 
caccatcaa agtcaaccg tgtctgtcg actcggctg 
gcgaggact tccggaaag agtcgatta tgctccgac 
caccggaaa acaggaact attcccttc agccagttt 
ggaattgtg cgacgttcc atgtaacgt gagggaatt 
acgaacgag ggttgattt agccaccag acatatcgg 
caaaggttg cgatcgtga cacatacag cgtccagtc 
tcagcgcaa attatgcgg ggcttcccc gtcggctat 
cgatctact tccgagtcc gggtgtcgg tgccgaatg 
gacgaagcc gctgatttc cgacgaggg acacgaatc 
ggatcagat gtttgaatt tgacggctc cagcactgc 
cgtttccat tccgaattc ggtcttgga tcataattt 
gccgtcaac gttcacgat agccacgtg gatgagaca 
tggacaaca taatgggca ggataaacg cgtcaagga 
acatatttt gtgttcggc tgacgacca taaaatgaa 
ccgccacca ctacgccct ctttgtccg caactgccg 
cctcatcct cctcctccg gcgcgactt ctccgaagg 
cgacctcaa catcttccg tccggcgga atggcgttg 
gaagatccc ccaggtctg cgacgacca atggcatca 
ttacgccgt caacgtgcg tttccccga cattgaact 
ctacgtcga cggtcaact cttccgttc cgagaagaa 
aaacccgga agtcatcga cgattggcc actccatcc 
cacgaaagg catcaacac cacgttgac agtcggcgc 
ctgctggca gggctccga caacaagat gaagcacca 
tctcaaggg ctatctggc cggcttgag cgtcctcgt 
tggcgagac ggaaaagcc cgaagtgtt gagctgtct 
gcatcagtg caaagagag tcttcaagt gcccgcaat 
ggaactcct tcaacctgg aatggaact gttgacgaa 
ttcggatct gacggaagt tacagtcga gggagataa 
tcgcacgaa tttggaaac tttggttcg gaagatcgg 
gtatgcgaa tagtcggga gtttccgac gccgggaag 
gaggaattt gaggctgac gacgacggt tacgtgcga 
taacgggaa gacggtcaa ggtgcccga tgcgcaaac 
ttatgtcat ggttttaag gccgcaaac gccctcgat 
caatctcaa tgggacggg gaacatggc gaggaaata 
cgaggattt caggatggg ggttcgcgt cctggccga 
catccacat atccgggga acaaaagct cgacaaatg 
ctcgttaac catctaccc taacttaaa ccccgacca 
tgagaactt aagcgttcc cgaagaaat gttaaaaag 
actcggaat ggcagctcg tgtatcaaa agatggtgt 
taccatcac cggatccga catggtctt caactacca 
acaagtact cagacaaat cgtctacac caaccgaaa 
acccgccta ctacctaaa ccgagtgtt caaactaac 
ctgctccga attaaacgg acgattcac gtcaaatga 
atacgtgca aactctcac tgtgatcca tcctcagcc 
caaagagcc catcgtagc tcatgcaac tgtgaacaa 
acacaatgt ggaattacg accagcgtc ggtcgtttc 
acgtgatta cattccttc ggaacattt gaatttggt 
cgcaagtgg gggatcaca tgctgttac aataatcgt 
cgtagtgtg cgtcggatt tttgttgtt tatgattgt 
cttgggtgt gatcaggat cagggcggc tcatcatag 
aaatgcatc cgaggaggc gcaagacag cgagatgac 
ttgggatga ttccgcgct gacaatcac cgtgaaccc 
aatggagaa cgctgaacg caaactgga aggccactc 
agccgacta cagcgaaag cgaccaatc cgattcgga 
cagttcatc ggacgacga caacatcga cggtccgtg 
cagaattcc cgactcttc ggacgacga agaggagca 
accggctcg tagtcgcaa acacgacta tcaaaatat 
cagccaact agaatggga ccattctac gatgtaaaa 
attccatct acaaaaaaa caaaaaaaa aacaagaaa 
cgggagcgg acgtttcac ctggtgagt tttatttgt 
aattcgcgc cgtttgaag cgtcacgaa ggcggtata 
tatattatt aaacataaa acacatacg caaaggttt 
gagccgtag cgaagcctt ttcgtacgg aaaaaaaaa 
tatatattt cgcggagcg gtcgcaaaa ctgccgaaa 
aaaacaatc agcgggagt tttgcgaac gcgtcgcga 
ttgatatca ataataatc gtccataat gttagagtt 
atacaagtt gtatatccc tttcactta gctattagt 
ataaaaaaa aagaaacag ctaggttgg ggtgtttta 
acgcccgct aagtaaata gctatttcc tcatccttt 
cgcttggaa tcgcctgtt tgtttcggt tatttaagg 
gcagccatt gtttttaac acgtagggt tagttttaa 
gatggaaag tctttcgtc ctttcgaag cttcgacaa 
taattcagc ttgtttttg tagtgcggt ggcagcggc 
tttctctaa accgaaaga ctttcttcc ttttttttt 
taaattatt gacctaacg tttaaggcc taggttaaa 
aagcaagta tttttccta ttttttaat taacctgaa 
cgctttatt ttgtactta cgtagtttt aatcactca 
cttcactcg aaatattcc tcgtattgt catatcaaa 
ttttgtaca ctgtctgtc gctggtttt ttcgaacgt 
ttcgttcca aacgcatca acaactagg caaaaatga 
ttgatttat ttagaatcg atgtttgtg gactctctc 
gcacaagtt catgtctct aaaataata atacaaaag 
gaggtgtac ataaagttt tgcagctaa atagtattt 
ttttgtatc gtagacgat gggtactta cgaataaat 
gaaatggtt tatttatta caagatgcg cggtaaatt 
attatataa aaggatgca atagtagtt gtgtataaa 
agaagaaca agtctatat catatttgc aatatgtaa 
gtgtattgt ttttcgtcg ccattttgt ttatacgtg 
ttggcatct tagaatagt agctgtgat gttgatgaa 
aaaatgaga atgttttag taagcacta ttcggttga 
tttgtttcc ggccgttcg actgactga tcgatgaat 
tggcgcgat tttctcgaa tttttattc aaaaactaa 
cctcaaaaa cgttgtata tttgattgt agaaagtgt 
aattattat agtaggcgg tgtgtccga aagtcgcgc 
ttgtttttt tcaaagtcg aactcagct gtcagatta 
ctattgaaa aagtaataa cactatttt tgacaagta 
cgtaacaga acgttacat tccatgcat atatttgta 
atctttgta tctttaaca gttacgagc tttgttgtg 
tgtttacaa atcagaaat caaataatc cgaatatct 
ttaggtgtc ataccacaa taattatgt aatacataa 
atgtataat aaataacaa atatagcc
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