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Gene information
General information
TC015622 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 3532566..3574532
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015622
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02498

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 3
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 7 (16 dpi)
    Days post injection



    Pupal morphology
    head slightly orientation irregular - Number of animals showing the phenotype: 9

    wings dv-orientation irregular - Number of animals showing the phenotype: 9

    wings potentially shortened - Number of animals showing the phenotype: 9
    Adult morphology
    flagellomeres shape irregular - Number of animals showing the phenotype: 7


    flagellomeres slightlynumber decreased - Number of animals showing the phenotype: 7

    elytra blistered - Number of animals showing the phenotype: 7


    iBeetle screen iB_06352

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    egg chamber mostly not present - Number of animals showing the phenotype: 3

    egg chamber number decreased - Number of animals showing the phenotype: 5
    tropharium sometimes shape irregular - Number of animals showing the phenotype: 5
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 9 (16 dpi)
    Days post injection



    Adult morphology
    flagellomeres number decreased - Number of animals showing the phenotype: 9


    funicle shortened - Number of animals showing the phenotype: 9

    elytra dorsal not closed - Number of animals showing the phenotype: 9

    legs tarsomere sometimes shortened - Number of animals showing the phenotype: 9


    legs tarsomere sometimes number decreased - Number of animals showing the phenotype: 3


    legs femur broader - Number of animals showing the phenotype: 9


    legs tibia slightly broader - Number of animals showing the phenotype: 9


    elytra blistered - Number of animals showing the phenotype: 2

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015622 RA
      MFWFAICAL LLTPIASST EPFDEYTAP KLDVDNLEN 
      GYHGLVKEN ETLVEVTPA IRAIGEQVC SFRIVNKHH 
      GEAPFEIVS KEDGYAELR ARRVLNCEK RRNYKFDIA 
      AVGCNGKQS VSATVHITV SDVNEYAPT FSEPSYVRQ 
      VDEGRIYSE IVRVEATDR DCTPKFGDV CKYEILTSD 
      QPFVIDMDG VIRNTEPLD YERSHNHIL SVVAYDCGM 
      RQSLPVMVT IKVNRVCRL GWRGLPERV DYAPTTGKQ 
      ELFPSASLE LCDVPCNVR ELRTRVDLA TRHIGKGCD 
      RDTYSVQSQ RKLCGASPS AIDLLPSPG VGAEWTKPL 
      ISDEGHESD QMFEFDGSS TAVSIPNSV LDHNLPSTF 
      TIATWMRHG QHNGQDKRV KEHILCSAD DHKMNRHHY 
      ALFVRNCRL ILLLRRDFS EGDLNIFRP AEWRWKIPQ 
      VCDDQWHHY AVNVRFPDI ELYVDGQLF RSEKKNPEV 
      IDDWPLHPT KGINTTLTV GACWQGSDN KMKHHLKGY 
      LAGLSVLVG ETEKPEVLS CLHQCKESL QVPAMELLQ 
      PGMELLTNS DLTEVTVEG DNRTNLETL VRKIGYANS 
      REFPTPGRR NLRLTTTVT CDNGKTVKV PDAQTYVMV 
      LRPQTPSIN LNGTGNMAR KYEDFRMGV RVLADIHIS 
      GEQKLDKCS LTIYPNLNP DHENLSVPE EMLKRLGMA 
      ARVSKDGVT ITGSDMVFN YQQVLRQIV YTNRKPAYY 
      LNRVFKLTC SELNGRFTS NEYVQTLTV IHPQPKEPI 
      VAHATVNKH NVELRPASV VSRDYIPSE HLNLVASGG 
      SHAVTIIVV VCVGFLLFM IVLGVIRIR AAHHRNASE 
      EAQDSEMTW DDSALTITV NPMENAERK LEGHSADYS 
      ESDQSDSDS SSDDDNIDG PCRIPDSSD DEEEQPARS 
      RKHDYQNIS QLEWDHSTM
    CDS sequence
    • >TC015622 RA
      atgttttgg ttcgcgatc tgtgccctc cttctgact 
      cccatcgct tcttcgacg gagccgttc gacgagtac 
      acagctccc aaattggac gtggacaat ttggagaac 
      ggctaccac ggcctcgtg aaagagaac gagacgttg 
      gtcgaagtg acgccggcg atacgcgcc atcggcgaa 
      caagtttgc agttttcgc atcgtaaac aagcaccat 
      ggcgaagct ccatttgag atcgtctcg aaagaggac 
      ggctatgcc gaactcagg gccagaagg gtgctcaac 
      tgcgagaaa agaaggaac tacaagttc gacattgct 
      gccgtcggc tgtaacggc aagcagagc gtcagcgcc 
      accgttcac ataaccgtc tcagatgtg aacgaatac 
      gcaccgact ttctccgaa ccaagctac gtaaggcaa 
      gtcgatgaa ggacgaatc tacagcgag attgttcgt 
      gttgaagcg accgatcgt gattgtacg ccgaagttc 
      ggcgatgtc tgcaaatat gaaatactt acgtcagat 
      cagcctttc gtcatcgac atggatggg gttattagg 
      aatacggag cctttggat tatgagagg tcgcataac 
      cacatactc agtgttgtg gcttacgac tgtggaatg 
      cgtcaaagc ttgcccgtc atggtcacc atcaaagtc 
      aaccgtgtc tgtcgactc ggctggcga ggacttccg 
      gaaagagtc gattatgct ccgaccacc ggaaaacag 
      gaactattc ccttcagcc agtttggaa ttgtgcgac 
      gttccatgt aacgtgagg gaattacga acgagggtt 
      gatttagcc accagacat atcggcaaa ggttgcgat 
      cgtgacaca tacagcgtc cagtctcag cgcaaatta 
      tgcggggct tccccgtcg gctatcgat ctacttccg 
      agtccgggt gtcggtgcc gaatggacg aagccgctg 
      atttccgac gagggacac gaatcggat cagatgttt 
      gaatttgac ggctccagc actgccgtt tccattccg 
      aattcggtc ttggatcat aatttgccg tcaacgttc 
      acgatagcc acgtggatg agacatgga caacataat 
      gggcaggat aaacgcgtc aaggaacat attttgtgt 
      tcggctgac gaccataaa atgaaccgc caccactac 
      gccctcttt gtccgcaac tgccgcctc atcctcctc 
      ctccggcgc gacttctcc gaaggcgac ctcaacatc 
      ttccgtccg gcggaatgg cgttggaag atcccccag 
      gtctgcgac gaccaatgg catcattac gccgtcaac 
      gtgcgtttc cccgacatt gaactctac gtcgacggt 
      caactcttc cgttccgag aagaaaaac ccggaagtc 
      atcgacgat tggccactc catcccacg aaaggcatc 
      aacaccacg ttgacagtc ggcgcctgc tggcagggc 
      tccgacaac aagatgaag caccatctc aagggctat 
      ctggccggc ttgagcgtc ctcgttggc gagacggaa 
      aagcccgaa gtgttgagc tgtctgcat cagtgcaaa 
      gagagtctt caagtgccc gcaatggaa ctccttcaa 
      cctggaatg gaactgttg acgaattcg gatctgacg 
      gaagttaca gtcgaggga gataatcgc acgaatttg 
      gaaactttg gttcggaag atcgggtat gcgaatagt 
      cgggagttt ccgacgccg ggaaggagg aatttgagg 
      ctgacgacg acggttacg tgcgataac gggaagacg 
      gtcaaggtg cccgatgcg caaacttat gtcatggtt 
      ttaaggccg caaacgccc tcgatcaat ctcaatggg 
      acggggaac atggcgagg aaatacgag gatttcagg 
      atgggggtt cgcgtcctg gccgacatc cacatatcc 
      ggggaacaa aagctcgac aaatgctcg ttaaccatc 
      taccctaac ttaaacccc gaccatgag aacttaagc 
      gttcccgaa gaaatgtta aaaagactc ggaatggca 
      gctcgtgta tcaaaagat ggtgttacc atcaccgga 
      tccgacatg gtcttcaac taccaacaa gtactcaga 
      caaatcgtc tacaccaac cgaaaaccc gcctactac 
      ctaaaccga gtgttcaaa ctaacctgc tccgaatta 
      aacggacga ttcacgtca aatgaatac gtgcaaact 
      ctcactgtg atccatcct cagcccaaa gagcccatc 
      gtagctcat gcaactgtg aacaaacac aatgtggaa 
      ttacgacca gcgtcggtc gtttcacgt gattacatt 
      ccttcggaa catttgaat ttggtcgca agtggggga 
      tcacatgct gttacaata atcgtcgta gtgtgcgtc 
      ggatttttg ttgtttatg attgtcttg ggtgtgatc 
      aggatcagg gcggctcat catagaaat gcatccgag 
      gaggcgcaa gacagcgag atgacttgg gatgattcc 
      gcgctgaca atcaccgtg aacccaatg gagaacgct 
      gaacgcaaa ctggaaggc cactcagcc gactacagc 
      gaaagcgac caatccgat tcggacagt tcatcggac 
      gacgacaac atcgacggt ccgtgcaga attcccgac 
      tcttcggac gacgaagag gagcaaccg gctcgtagt 
      cgcaaacac gactatcaa aatatcagc caactagaa 
      tgggaccat tctacgatg taa
    mRNA sequence
    • >TC015622 RA
      gggagacag tcaatgaaa acctagccg tccatgacg 
      acacaattt agtgagtcg gtatgaaaa tcgtgtgtt 
      tatcatcat aaaaaagcg agttgtggt gtgtgcccc 
      gtgcgcgta caagacccc catcagacg tttttgtcg 
      cctcgccgc cgaaatgtt ttggttcgc gatctgtgc 
      cctccttct gactcccat cgcttcttc gacggagcc 
      gttcgacga gtacacagc tcccaaatt ggacgtgga 
      caatttgga gaacggcta ccacggcct cgtgaaaga 
      gaacgagac gttggtcga agtgacgcc ggcgatacg 
      cgccatcgg cgaacaagt ttgcagttt tcgcatcgt 
      aaacaagca ccatggcga agctccatt tgagatcgt 
      ctcgaaaga ggacggcta tgccgaact cagggccag 
      aagggtgct caactgcga gaaaagaag gaactacaa 
      gttcgacat tgctgccgt cggctgtaa cggcaagca 
      gagcgtcag cgccaccgt tcacataac cgtctcaga 
      tgtgaacga atacgcacc gactttctc cgaaccaag 
      ctacgtaag gcaagtcga tgaaggacg aatctacag 
      cgagattgt tcgtgttga agcgaccga tcgtgattg 
      tacgccgaa gttcggcga tgtctgcaa atatgaaat 
      acttacgtc agatcagcc tttcgtcat cgacatgga 
      tggggttat taggaatac ggagccttt ggattatga 
      gaggtcgca taaccacat actcagtgt tgtggctta 
      cgactgtgg aatgcgtca aagcttgcc cgtcatggt 
      caccatcaa agtcaaccg tgtctgtcg actcggctg 
      gcgaggact tccggaaag agtcgatta tgctccgac 
      caccggaaa acaggaact attcccttc agccagttt 
      ggaattgtg cgacgttcc atgtaacgt gagggaatt 
      acgaacgag ggttgattt agccaccag acatatcgg 
      caaaggttg cgatcgtga cacatacag cgtccagtc 
      tcagcgcaa attatgcgg ggcttcccc gtcggctat 
      cgatctact tccgagtcc gggtgtcgg tgccgaatg 
      gacgaagcc gctgatttc cgacgaggg acacgaatc 
      ggatcagat gtttgaatt tgacggctc cagcactgc 
      cgtttccat tccgaattc ggtcttgga tcataattt 
      gccgtcaac gttcacgat agccacgtg gatgagaca 
      tggacaaca taatgggca ggataaacg cgtcaagga 
      acatatttt gtgttcggc tgacgacca taaaatgaa 
      ccgccacca ctacgccct ctttgtccg caactgccg 
      cctcatcct cctcctccg gcgcgactt ctccgaagg 
      cgacctcaa catcttccg tccggcgga atggcgttg 
      gaagatccc ccaggtctg cgacgacca atggcatca 
      ttacgccgt caacgtgcg tttccccga cattgaact 
      ctacgtcga cggtcaact cttccgttc cgagaagaa 
      aaacccgga agtcatcga cgattggcc actccatcc 
      cacgaaagg catcaacac cacgttgac agtcggcgc 
      ctgctggca gggctccga caacaagat gaagcacca 
      tctcaaggg ctatctggc cggcttgag cgtcctcgt 
      tggcgagac ggaaaagcc cgaagtgtt gagctgtct 
      gcatcagtg caaagagag tcttcaagt gcccgcaat 
      ggaactcct tcaacctgg aatggaact gttgacgaa 
      ttcggatct gacggaagt tacagtcga gggagataa 
      tcgcacgaa tttggaaac tttggttcg gaagatcgg 
      gtatgcgaa tagtcggga gtttccgac gccgggaag 
      gaggaattt gaggctgac gacgacggt tacgtgcga 
      taacgggaa gacggtcaa ggtgcccga tgcgcaaac 
      ttatgtcat ggttttaag gccgcaaac gccctcgat 
      caatctcaa tgggacggg gaacatggc gaggaaata 
      cgaggattt caggatggg ggttcgcgt cctggccga 
      catccacat atccgggga acaaaagct cgacaaatg 
      ctcgttaac catctaccc taacttaaa ccccgacca 
      tgagaactt aagcgttcc cgaagaaat gttaaaaag 
      actcggaat ggcagctcg tgtatcaaa agatggtgt 
      taccatcac cggatccga catggtctt caactacca 
      acaagtact cagacaaat cgtctacac caaccgaaa 
      acccgccta ctacctaaa ccgagtgtt caaactaac 
      ctgctccga attaaacgg acgattcac gtcaaatga 
      atacgtgca aactctcac tgtgatcca tcctcagcc 
      caaagagcc catcgtagc tcatgcaac tgtgaacaa 
      acacaatgt ggaattacg accagcgtc ggtcgtttc 
      acgtgatta cattccttc ggaacattt gaatttggt 
      cgcaagtgg gggatcaca tgctgttac aataatcgt 
      cgtagtgtg cgtcggatt tttgttgtt tatgattgt 
      cttgggtgt gatcaggat cagggcggc tcatcatag 
      aaatgcatc cgaggaggc gcaagacag cgagatgac 
      ttgggatga ttccgcgct gacaatcac cgtgaaccc 
      aatggagaa cgctgaacg caaactgga aggccactc 
      agccgacta cagcgaaag cgaccaatc cgattcgga 
      cagttcatc ggacgacga caacatcga cggtccgtg 
      cagaattcc cgactcttc ggacgacga agaggagca 
      accggctcg tagtcgcaa acacgacta tcaaaatat 
      cagccaact agaatggga ccattctac gatgtaaaa 
      attccatct acaaaaaaa caaaaaaaa aacaagaaa 
      cgggagcgg acgtttcac ctggtgagt tttatttgt 
      aattcgcgc cgtttgaag cgtcacgaa ggcggtata 
      tatattatt aaacataaa acacatacg caaaggttt 
      gagccgtag cgaagcctt ttcgtacgg aaaaaaaaa 
      tatatattt cgcggagcg gtcgcaaaa ctgccgaaa 
      aaaacaatc agcgggagt tttgcgaac gcgtcgcga 
      ttgatatca ataataatc gtccataat gttagagtt 
      atacaagtt gtatatccc tttcactta gctattagt 
      ataaaaaaa aagaaacag ctaggttgg ggtgtttta 
      acgcccgct aagtaaata gctatttcc tcatccttt 
      cgcttggaa tcgcctgtt tgtttcggt tatttaagg 
      gcagccatt gtttttaac acgtagggt tagttttaa 
      gatggaaag tctttcgtc ctttcgaag cttcgacaa 
      taattcagc ttgtttttg tagtgcggt ggcagcggc 
      tttctctaa accgaaaga ctttcttcc ttttttttt 
      taaattatt gacctaacg tttaaggcc taggttaaa 
      aagcaagta tttttccta ttttttaat taacctgaa 
      cgctttatt ttgtactta cgtagtttt aatcactca 
      cttcactcg aaatattcc tcgtattgt catatcaaa 
      ttttgtaca ctgtctgtc gctggtttt ttcgaacgt 
      ttcgttcca aacgcatca acaactagg caaaaatga 
      ttgatttat ttagaatcg atgtttgtg gactctctc 
      gcacaagtt catgtctct aaaataata atacaaaag 
      gaggtgtac ataaagttt tgcagctaa atagtattt 
      ttttgtatc gtagacgat gggtactta cgaataaat 
      gaaatggtt tatttatta caagatgcg cggtaaatt 
      attatataa aaggatgca atagtagtt gtgtataaa 
      agaagaaca agtctatat catatttgc aatatgtaa 
      gtgtattgt ttttcgtcg ccattttgt ttatacgtg 
      ttggcatct tagaatagt agctgtgat gttgatgaa 
      aaaatgaga atgttttag taagcacta ttcggttga 
      tttgtttcc ggccgttcg actgactga tcgatgaat 
      tggcgcgat tttctcgaa tttttattc aaaaactaa 
      cctcaaaaa cgttgtata tttgattgt agaaagtgt 
      aattattat agtaggcgg tgtgtccga aagtcgcgc 
      ttgtttttt tcaaagtcg aactcagct gtcagatta 
      ctattgaaa aagtaataa cactatttt tgacaagta 
      cgtaacaga acgttacat tccatgcat atatttgta 
      atctttgta tctttaaca gttacgagc tttgttgtg 
      tgtttacaa atcagaaat caaataatc cgaatatct 
      ttaggtgtc ataccacaa taattatgt aatacataa 
      atgtataat aaataacaa atatagcc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY