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Gene information
General information
TC015591 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid40: 2124682..2129076
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015591
    iBeetle screen iB_02496
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015591 RA
      MSVILENQP IKVIDPSEL LRPEPGFAK KPVGQFRDY 
      SVDENDPIK ERVRKTYKE MHTYQTVEF VQEKIKTWT 
      KFNHFKSTI KKALIRLND LVDESDPDV DLPNIVHAF 
      QTAERIRKD YPDLDWFQL TGLIHDLGK VMAFYGEPQ 
      WAVVGDTFV VGCDWGKDI VYRDTSFVD NVDGKNPKY 
      NTKYGIYKP NCGLENLLL SWGHDEYLY RVLVHNKST 
      LPKQALWMI RFHSFYPWH SSNNYQHLT TKEDEEEIK 
      DWVLKFNQY DLYTKSTEI PDIEALWPY YEKLIDKYI 
      PGVLEW
    CDS sequence
    • >TC015591 RA
      atgagcgtt atcctggaa aatcagccg atcaaagtg 
      atcgacccg tcggaattg ctgcggcca gaaccaggc 
      tttgccaaa aaacccgta ggacaattc cgggactat 
      tcggttgac gaaaatgat cccatcaag gaaagagtc 
      cgaaaaacg tacaaggaa atgcacacg taccagacc 
      gtggagttc gttcaagaa aaaataaaa acttggacc 
      aagttcaac cacttcaaa tcaacaatc aaaaaagcc 
      ctaatccgc cttaacgac ttggttgat gaaagtgat 
      cccgatgtt gatcttccc aacatcgtc catgccttc 
      caaacggct gaaagaata cgaaaagac tatcctgac 
      ttagactgg ttccaacta actggacta attcatgat 
      ttgggcaaa gttatggca ttctatggg gaaccacag 
      tgggctgtt gtcggcgac accttcgta gtgggctgt 
      gactggggc aaagatatt gtctataga gacacttct 
      ttcgtcgat aacgtcgat ggaaaaaat cccaaatat 
      aatacaaaa tatgggatt tacaaacca aactgtgga 
      ctggaaaat ttgttactt tcttggggt catgatgag 
      tacttgtat cgcgttttg gtgcataat aaatcaact 
      cttccgaaa caagccttg tggatgatt cgtttccat 
      agtttttat ccttggcac tcaagtaac aattaccaa 
      catttaaca actaaggaa gatgaggag gaaatcaag 
      gattgggtt ttgaaattt aaccaatat gatttatac 
      accaagagc accgaaatc ccggacatt gaagcactg 
      tggccctat tacgaaaaa ttgatcgat aaatacata 
      ccgggagtg ttggaatgg taa
    mRNA sequence
    • >TC015591 RA
      taactcatt tagtttttt tcgagatct cgtcaattg 
      tcaccgaat cgtcaaaga tgagcgtta tcctggaaa 
      atcagccga tcaaagtga tcgacccgt cggaattgc 
      tgcggccag aaccaggct ttgccaaaa aacccgtag 
      gacaattcc gggactatt cggttgacg aaaatgatc 
      ccatcaagg aaagagtcc gaaaaacgt acaaggaaa 
      tgcacacgt accagaccg tggagttcg ttcaagaaa 
      aaataaaaa cttggacca agttcaacc acttcaaat 
      caacaatca aaaaagccc taatccgcc ttaacgact 
      tggttgatg aaagtgatc ccgatgttg atcttccca 
      acatcgtcc atgccttcc aaacggctg aaagaatac 
      gaaaagact atcctgact tagactggt tccaactaa 
      ctggactaa ttcatgatt tgggcaaag ttatggcat 
      tctatgggg aaccacagt gggctgttg tcggcgaca 
      ccttcgtag tgggctgtg actggggca aagatattg 
      tctatagag acacttctt tcgtcgata acgtcgatg 
      gaaaaaatc ccaaatata atacaaaat atgggattt 
      acaaaccaa actgtggac tggaaaatt tgttacttt 
      cttggggtc atgatgagt acttgtatc gcgttttgg 
      tgcataata aatcaactc ttccgaaac aagccttgt 
      ggatgattc gtttccata gtttttatc cttggcact 
      caagtaaca attaccaac atttaacaa ctaaggaag 
      atgaggagg aaatcaagg attgggttt tgaaattta 
      accaatatg atttataca ccaagagca ccgaaatcc 
      cggacattg aagcactgt ggccctatt acgaaaaat 
      tgatcgata aatacatac cgggagtgt tggaatggt 
      aaataactt tcgagaaaa cacttttat taaaaaata 
      atctacatg tacatac