Status
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Gene information
General information
TC015584 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 2704738..2707322
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC015584
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02495

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    larva lethality late (L7) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015584 RA
      MGKKEQFLK RKLEKSAEE SDEPDSGAE SVENEELLN 
VVNGEDADS SDSDGEIEE SDEDLNDSD DEIEESDED 
DSNTTDEES DENELENEE NAAEDTANP ARDEYSSHD 
TSDEEDIRT TVGNIPMNW YDDFKHLGY DWDGNQIIK 
PESGDLLDT FLKRMEDPD FWRTVKDPQ TGQNVVLSD 
ADIDLIKRL KANQIPDAT FDEYAPWIE WFTSEVMKT 
PLRKFPEHK RSFLPSKSD AKKVSKLVH ALKMGWIKT 
RAEAEKLRA KKEPEFYML WATDDQAED MRRIHKHIP 
APKRLLPGH AESYNPPPE YLFDEKELK QWNKHKNAP 
WKRKLHFVP QKFESLRHV PAYPRYIKE RFLRCLDLY 
LCPRGIKMK LNIEPEALV PKLPSPKDL QPFPTTLSI 
EYKGHGDMV RTISTDKIG QYLVSGSDD GTIKLWEVN 
TGRCLKTID TGGVVRSVE WCPNPALSL ILVAASNRV 
LLINPHLGD TLITSKTDS VLETAPQSD AIVSERIKT 
TVEWSESID PKEYTQGVR IALKHFKEV AQVTWHGKG 
DYFATVMPD GLNRSVLIS QISRRRSQI PFTKSKGLV 
QCVLFHPTK PCLFVATQR HVRIYDLVK QTLMKKLLT 
NSKWISTMA IHPGGDNLL VGTYDRKML WFDLDLSTK 
PYQTLRLHG TAVRGVAFH KRYPLFASG SDDKSLIVC 
HGMVYNDLL KNPLIVPLK RLQDHETNN DFGIFGVLF 
HPLQPWVFS SGADGTIKL YSN
    CDS sequence
    • >TC015584 RA
      atgggcaaa aaagaacag tttttaaaa cgaaaatta 
gaaaaatcg gccgaggaa agtgacgag cctgattct 
ggtgcagag tctgtcgag aatgaggag cttttgaat 
gttgtaaat ggggaagac gccgacagc tccgactcc 
gatggcgaa attgaagaa agcgacgaa gatttgaac 
gactccgat gatgaaatt gaagaaagc gatgaagac 
gattcaaac accaccgat gaagaaagc gacgaaaac 
gagctggag aatgaagaa aacgccgcc gaagacacc 
gccaaccca gcaagagac gaatactcc tcgcacgac 
acctccgac gaggaggac atccgcacc accgtgggc 
aacatcccc atgaactgg tacgacgac ttcaaacac 
ctgggctac gactgggac ggcaaccag atcatcaag 
cccgagtcg ggcgacctc ctcgacacc ttcctcaag 
cgcatggaa gaccccgac ttctggcgc accgtcaaa 
gacccgcaa accggccaa aacgtcgtc ctctccgac 
gccgacatc gacctcatc aagcgcctc aaagccaac 
caaatcccg gacgccacc ttcgacgaa tacgccccg 
tggatcgaa tggttcacg tccgaggtg atgaaaaca 
ccgctccgg aaattcccc gaacacaag cgctccttc 
ctgccgagc aaaagcgac gccaagaaa gtctccaag 
ctggtgcac gccctgaag atgggctgg atcaagacg 
cgggccgag gccgaaaaa ctgcgcgcg aagaaagag 
cccgagttt tacatgttg tgggccacc gatgaccag 
gccgaagac atgaggcgc attcacaaa cacataccg 
gcaccgaag cgcctcctg cctggccat gccgagtca 
tataaccca ccaccggag tatttattc gatgaaaaa 
gaactcaaa cagtggaat aaacataaa aacgccccc 
tggaaacgg aaattacat ttcgtgcca caaaagttc 
gagtcactg agacacgtt ccagcgtac cctcgatac 
ataaaagag aggtttttg cgctgtttg gacttgtat 
ttgtgcccc cgtgggatt aaaatgaaa ctgaacatc 
gagcctgaa gctttggtg cccaaactc ccgagtccg 
aaagaccta caaccgttc cccaccact ctctcgatt 
gaatacaag ggacatggc gacatggtc cgtaccata 
agtaccgat aaaatcggc cagtaccta gtgagcggt 
tccgatgat ggtaccatc aaattatgg gaggttaat 
accgggcgt tgcctcaaa acgattgac acggggggc 
gtggtccgt tcagtcgag tggtgccct aaccccgcc 
ctttccttg atccttgtc gccgcctcc aaccgtgtc 
ctgctaatc aacccgcac cttggcgac actttgatc 
acttccaag acggattcg gtcctcgaa accgccccc 
caaagcgac gcaatcgtg agcgaacga atcaaaacc 
accgtcgaa tggtcggaa tcaatcgac ccgaaagag 
tacactcaa ggcgtccga atcgccctc aaacacttc 
aaggaagtg gcccaggtc acgtggcac ggcaagggc 
gactacttc gccactgtg atgcccgac ggcctcaac 
cgctcggtt ctaatcagt cagatcagc cgtcgcagg 
tcgcaaatc cccttcacc aagtccaaa ggcctagtc 
cagtgtgtt ttattccac ccgaccaaa ccctgcctc 
tttgtggcc acgcagcga cacgtccgc atctacgac 
ctggtgaag caaaccttg atgaagaag ctgctgacg 
aactccaag tggatttcc accatggcc atacaccca 
ggcggtgat aatcttcta gtggggacg tacgatcgc 
aaaatgttg tggtttgac ctggatttg tccaccaag 
ccgtaccag accttgagg cttcacggg acggcggtc 
cgcggggtg gccttccat aagcggtac ccgcttttc 
gcctccggg tccgatgat aagagcttg attgtgtgt 
cacgggatg gtgtacaac gacttgctg aaaaatcca 
ctgattgtg cccctgaaa cggctgcag gatcacgaa 
acgaacaac gattttggc atctttggg gtgcttttc 
cacccgttg caaccgtgg gtgttctcc tcgggggcc 
gacgggacc atcaagttg tattctaat taa
    mRNA sequence
    • >TC015584 RA
      ttccgcttc tataaaagt gttattcta gggacatgt 
ggtaactta acccaactt tcttagcat gtgtggccg 
ataaacaaa cgtaaaaat gggcaaaaa agaacagtt 
tttaaaacg aaaattaga aaaatcggc cgaggaaag 
tgacgagcc tgattctgg tgcagagtc tgtcgagaa 
tgaggagct tttgaatgt tgtaaatgg ggaagacgc 
cgacagctc cgactccga tggcgaaat tgaagaaag 
cgacgaaga tttgaacga ctccgatga tgaaattga 
agaaagcga tgaagacga ttcaaacac caccgatga 
agaaagcga cgaaaacga gctggagaa tgaagaaaa 
cgccgccga agacaccgc caacccagc aagagacga 
atactcctc gcacgacac ctccgacga ggaggacat 
ccgcaccac cgtgggcaa catccccat gaactggta 
cgacgactt caaacacct gggctacga ctgggacgg 
caaccagat catcaagcc cgagtcggg cgacctcct 
cgacacctt cctcaagcg catggaaga ccccgactt 
ctggcgcac cgtcaaaga cccgcaaac cggccaaaa 
cgtcgtcct ctccgacgc cgacatcga cctcatcaa 
gcgcctcaa agccaacca aatcccgga cgccacctt 
cgacgaata cgccccgtg gatcgaatg gttcacgtc 
cgaggtgat gaaaacacc gctccggaa attccccga 
acacaagcg ctccttcct gccgagcaa aagcgacgc 
caagaaagt ctccaagct ggtgcacgc cctgaagat 
gggctggat caagacgcg ggccgaggc cgaaaaact 
gcgcgcgaa gaaagagcc cgagtttta catgttgtg 
ggccaccga tgaccaggc cgaagacat gaggcgcat 
tcacaaaca cataccggc accgaagcg cctcctgcc 
tggccatgc cgagtcata taacccacc accggagta 
tttattcga tgaaaaaga actcaaaca gtggaataa 
acataaaaa cgccccctg gaaacggaa attacattt 
cgtgccaca aaagttcga gtcactgag acacgttcc 
agcgtaccc tcgatacat aaaagagag gtttttgcg 
ctgtttgga cttgtattt gtgcccccg tgggattaa 
aatgaaact gaacatcga gcctgaagc tttggtgcc 
caaactccc gagtccgaa agacctaca accgttccc 
caccactct ctcgattga atacaaggg acatggcga 
catggtccg taccataag taccgataa aatcggcca 
gtacctagt gagcggttc cgatgatgg taccatcaa 
attatggga ggttaatac cgggcgttg cctcaaaac 
gattgacac ggggggcgt ggtccgttc agtcgagtg 
gtgccctaa ccccgccct ttccttgat ccttgtcgc 
cgcctccaa ccgtgtcct gctaatcaa cccgcacct 
tggcgacac tttgatcac ttccaagac ggattcggt 
cctcgaaac cgcccccca aagcgacgc aatcgtgag 
cgaacgaat caaaaccac cgtcgaatg gtcggaatc 
aatcgaccc gaaagagta cactcaagg cgtccgaat 
cgccctcaa acacttcaa ggaagtggc ccaggtcac 
gtggcacgg caagggcga ctacttcgc cactgtgat 
gcccgacgg cctcaaccg ctcggttct aatcagtca 
gatcagccg tcgcaggtc gcaaatccc cttcaccaa 
gtccaaagg cctagtcca gtgtgtttt attccaccc 
gaccaaacc ctgcctctt tgtggccac gcagcgaca 
cgtccgcat ctacgacct ggtgaagca aaccttgat 
gaagaagct gctgacgaa ctccaagtg gatttccac 
catggccat acacccagg cggtgataa tcttctagt 
ggggacgta cgatcgcaa aatgttgtg gtttgacct 
ggatttgtc caccaagcc gtaccagac cttgaggct 
tcacgggac ggcggtccg cggggtggc cttccataa 
gcggtaccc gcttttcgc ctccgggtc cgatgataa 
gagcttgat tgtgtgtca cgggatggt gtacaacga 
cttgctgaa aaatccact gattgtgcc cctgaaacg 
gctgcagga tcacgaaac gaacaacga ttttggcat 
ctttggggt gcttttcca cccgttgca accgtgggt 
gttctcctc gggggccga cgggaccat caagttgta 
ttctaatta ataaatttt tacggaaaa tgttgtgtt 
gtttggaaa tgcgggaca ggttgagga ggaagattg 
ccaagaaaa tgttcggaa gttggaaga agacaggtc 
gagacaag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY