General information | |||
TC015584 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007421.3: 2704738..2707322 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC015584 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_02495 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 2 (16 dpi)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
larva lethality late (L7) - Number of animals showing the phenotype: 1 (19 dpi)
- >TC015584 RA
MGKKEQFLK RKLEKSAEE SDEPDSGAE SVENEELLN VVNGEDADS SDSDGEIEE SDEDLNDSD DEIEESDED DSNTTDEES DENELENEE NAAEDTANP ARDEYSSHD TSDEEDIRT TVGNIPMNW YDDFKHLGY DWDGNQIIK PESGDLLDT FLKRMEDPD FWRTVKDPQ TGQNVVLSD ADIDLIKRL KANQIPDAT FDEYAPWIE WFTSEVMKT PLRKFPEHK RSFLPSKSD AKKVSKLVH ALKMGWIKT RAEAEKLRA KKEPEFYML WATDDQAED MRRIHKHIP APKRLLPGH AESYNPPPE YLFDEKELK QWNKHKNAP WKRKLHFVP QKFESLRHV PAYPRYIKE RFLRCLDLY LCPRGIKMK LNIEPEALV PKLPSPKDL QPFPTTLSI EYKGHGDMV RTISTDKIG QYLVSGSDD GTIKLWEVN TGRCLKTID TGGVVRSVE WCPNPALSL ILVAASNRV LLINPHLGD TLITSKTDS VLETAPQSD AIVSERIKT TVEWSESID PKEYTQGVR IALKHFKEV AQVTWHGKG DYFATVMPD GLNRSVLIS QISRRRSQI PFTKSKGLV QCVLFHPTK PCLFVATQR HVRIYDLVK QTLMKKLLT NSKWISTMA IHPGGDNLL VGTYDRKML WFDLDLSTK PYQTLRLHG TAVRGVAFH KRYPLFASG SDDKSLIVC HGMVYNDLL KNPLIVPLK RLQDHETNN DFGIFGVLF HPLQPWVFS SGADGTIKL YSN
- >TC015584 RA
atgggcaaa aaagaacag tttttaaaa cgaaaatta gaaaaatcg gccgaggaa agtgacgag cctgattct ggtgcagag tctgtcgag aatgaggag cttttgaat gttgtaaat ggggaagac gccgacagc tccgactcc gatggcgaa attgaagaa agcgacgaa gatttgaac gactccgat gatgaaatt gaagaaagc gatgaagac gattcaaac accaccgat gaagaaagc gacgaaaac gagctggag aatgaagaa aacgccgcc gaagacacc gccaaccca gcaagagac gaatactcc tcgcacgac acctccgac gaggaggac atccgcacc accgtgggc aacatcccc atgaactgg tacgacgac ttcaaacac ctgggctac gactgggac ggcaaccag atcatcaag cccgagtcg ggcgacctc ctcgacacc ttcctcaag cgcatggaa gaccccgac ttctggcgc accgtcaaa gacccgcaa accggccaa aacgtcgtc ctctccgac gccgacatc gacctcatc aagcgcctc aaagccaac caaatcccg gacgccacc ttcgacgaa tacgccccg tggatcgaa tggttcacg tccgaggtg atgaaaaca ccgctccgg aaattcccc gaacacaag cgctccttc ctgccgagc aaaagcgac gccaagaaa gtctccaag ctggtgcac gccctgaag atgggctgg atcaagacg cgggccgag gccgaaaaa ctgcgcgcg aagaaagag cccgagttt tacatgttg tgggccacc gatgaccag gccgaagac atgaggcgc attcacaaa cacataccg gcaccgaag cgcctcctg cctggccat gccgagtca tataaccca ccaccggag tatttattc gatgaaaaa gaactcaaa cagtggaat aaacataaa aacgccccc tggaaacgg aaattacat ttcgtgcca caaaagttc gagtcactg agacacgtt ccagcgtac cctcgatac ataaaagag aggtttttg cgctgtttg gacttgtat ttgtgcccc cgtgggatt aaaatgaaa ctgaacatc gagcctgaa gctttggtg cccaaactc ccgagtccg aaagaccta caaccgttc cccaccact ctctcgatt gaatacaag ggacatggc gacatggtc cgtaccata agtaccgat aaaatcggc cagtaccta gtgagcggt tccgatgat ggtaccatc aaattatgg gaggttaat accgggcgt tgcctcaaa acgattgac acggggggc gtggtccgt tcagtcgag tggtgccct aaccccgcc ctttccttg atccttgtc gccgcctcc aaccgtgtc ctgctaatc aacccgcac cttggcgac actttgatc acttccaag acggattcg gtcctcgaa accgccccc caaagcgac gcaatcgtg agcgaacga atcaaaacc accgtcgaa tggtcggaa tcaatcgac ccgaaagag tacactcaa ggcgtccga atcgccctc aaacacttc aaggaagtg gcccaggtc acgtggcac ggcaagggc gactacttc gccactgtg atgcccgac ggcctcaac cgctcggtt ctaatcagt cagatcagc cgtcgcagg tcgcaaatc cccttcacc aagtccaaa ggcctagtc cagtgtgtt ttattccac ccgaccaaa ccctgcctc tttgtggcc acgcagcga cacgtccgc atctacgac ctggtgaag caaaccttg atgaagaag ctgctgacg aactccaag tggatttcc accatggcc atacaccca ggcggtgat aatcttcta gtggggacg tacgatcgc aaaatgttg tggtttgac ctggatttg tccaccaag ccgtaccag accttgagg cttcacggg acggcggtc cgcggggtg gccttccat aagcggtac ccgcttttc gcctccggg tccgatgat aagagcttg attgtgtgt cacgggatg gtgtacaac gacttgctg aaaaatcca ctgattgtg cccctgaaa cggctgcag gatcacgaa acgaacaac gattttggc atctttggg gtgcttttc cacccgttg caaccgtgg gtgttctcc tcgggggcc gacgggacc atcaagttg tattctaat taa
- >TC015584 RA
ttccgcttc tataaaagt gttattcta gggacatgt ggtaactta acccaactt tcttagcat gtgtggccg ataaacaaa cgtaaaaat gggcaaaaa agaacagtt tttaaaacg aaaattaga aaaatcggc cgaggaaag tgacgagcc tgattctgg tgcagagtc tgtcgagaa tgaggagct tttgaatgt tgtaaatgg ggaagacgc cgacagctc cgactccga tggcgaaat tgaagaaag cgacgaaga tttgaacga ctccgatga tgaaattga agaaagcga tgaagacga ttcaaacac caccgatga agaaagcga cgaaaacga gctggagaa tgaagaaaa cgccgccga agacaccgc caacccagc aagagacga atactcctc gcacgacac ctccgacga ggaggacat ccgcaccac cgtgggcaa catccccat gaactggta cgacgactt caaacacct gggctacga ctgggacgg caaccagat catcaagcc cgagtcggg cgacctcct cgacacctt cctcaagcg catggaaga ccccgactt ctggcgcac cgtcaaaga cccgcaaac cggccaaaa cgtcgtcct ctccgacgc cgacatcga cctcatcaa gcgcctcaa agccaacca aatcccgga cgccacctt cgacgaata cgccccgtg gatcgaatg gttcacgtc cgaggtgat gaaaacacc gctccggaa attccccga acacaagcg ctccttcct gccgagcaa aagcgacgc caagaaagt ctccaagct ggtgcacgc cctgaagat gggctggat caagacgcg ggccgaggc cgaaaaact gcgcgcgaa gaaagagcc cgagtttta catgttgtg ggccaccga tgaccaggc cgaagacat gaggcgcat tcacaaaca cataccggc accgaagcg cctcctgcc tggccatgc cgagtcata taacccacc accggagta tttattcga tgaaaaaga actcaaaca gtggaataa acataaaaa cgccccctg gaaacggaa attacattt cgtgccaca aaagttcga gtcactgag acacgttcc agcgtaccc tcgatacat aaaagagag gtttttgcg ctgtttgga cttgtattt gtgcccccg tgggattaa aatgaaact gaacatcga gcctgaagc tttggtgcc caaactccc gagtccgaa agacctaca accgttccc caccactct ctcgattga atacaaggg acatggcga catggtccg taccataag taccgataa aatcggcca gtacctagt gagcggttc cgatgatgg taccatcaa attatggga ggttaatac cgggcgttg cctcaaaac gattgacac ggggggcgt ggtccgttc agtcgagtg gtgccctaa ccccgccct ttccttgat ccttgtcgc cgcctccaa ccgtgtcct gctaatcaa cccgcacct tggcgacac tttgatcac ttccaagac ggattcggt cctcgaaac cgcccccca aagcgacgc aatcgtgag cgaacgaat caaaaccac cgtcgaatg gtcggaatc aatcgaccc gaaagagta cactcaagg cgtccgaat cgccctcaa acacttcaa ggaagtggc ccaggtcac gtggcacgg caagggcga ctacttcgc cactgtgat gcccgacgg cctcaaccg ctcggttct aatcagtca gatcagccg tcgcaggtc gcaaatccc cttcaccaa gtccaaagg cctagtcca gtgtgtttt attccaccc gaccaaacc ctgcctctt tgtggccac gcagcgaca cgtccgcat ctacgacct ggtgaagca aaccttgat gaagaagct gctgacgaa ctccaagtg gatttccac catggccat acacccagg cggtgataa tcttctagt ggggacgta cgatcgcaa aatgttgtg gtttgacct ggatttgtc caccaagcc gtaccagac cttgaggct tcacgggac ggcggtccg cggggtggc cttccataa gcggtaccc gcttttcgc ctccgggtc cgatgataa gagcttgat tgtgtgtca cgggatggt gtacaacga cttgctgaa aaatccact gattgtgcc cctgaaacg gctgcagga tcacgaaac gaacaacga ttttggcat ctttggggt gcttttcca cccgttgca accgtgggt gttctcctc gggggccga cgggaccat caagttgta ttctaatta ataaatttt tacggaaaa tgttgtgtt gtttggaaa tgcgggaca ggttgagga ggaagattg ccaagaaaa tgttcggaa gttggaaga agacaggtc gagacaag
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
