Status
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Gene information
General information
TC015584 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 2704738..2707322
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015584
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02495

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection



    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015584 RA
      MGKKEQFLK RKLEKSAEE SDEPDSGAE SVENEELLN 
      VVNGEDADS SDSDGEIEE SDEDLNDSD DEIEESDED 
      DSNTTDEES DENELENEE NAAEDTANP ARDEYSSHD 
      TSDEEDIRT TVGNIPMNW YDDFKHLGY DWDGNQIIK 
      PESGDLLDT FLKRMEDPD FWRTVKDPQ TGQNVVLSD 
      ADIDLIKRL KANQIPDAT FDEYAPWIE WFTSEVMKT 
      PLRKFPEHK RSFLPSKSD AKKVSKLVH ALKMGWIKT 
      RAEAEKLRA KKEPEFYML WATDDQAED MRRIHKHIP 
      APKRLLPGH AESYNPPPE YLFDEKELK QWNKHKNAP 
      WKRKLHFVP QKFESLRHV PAYPRYIKE RFLRCLDLY 
      LCPRGIKMK LNIEPEALV PKLPSPKDL QPFPTTLSI 
      EYKGHGDMV RTISTDKIG QYLVSGSDD GTIKLWEVN 
      TGRCLKTID TGGVVRSVE WCPNPALSL ILVAASNRV 
      LLINPHLGD TLITSKTDS VLETAPQSD AIVSERIKT 
      TVEWSESID PKEYTQGVR IALKHFKEV AQVTWHGKG 
      DYFATVMPD GLNRSVLIS QISRRRSQI PFTKSKGLV 
      QCVLFHPTK PCLFVATQR HVRIYDLVK QTLMKKLLT 
      NSKWISTMA IHPGGDNLL VGTYDRKML WFDLDLSTK 
      PYQTLRLHG TAVRGVAFH KRYPLFASG SDDKSLIVC 
      HGMVYNDLL KNPLIVPLK RLQDHETNN DFGIFGVLF 
      HPLQPWVFS SGADGTIKL YSN
    CDS sequence
    • >TC015584 RA
      atgggcaaa aaagaacag tttttaaaa cgaaaatta 
      gaaaaatcg gccgaggaa agtgacgag cctgattct 
      ggtgcagag tctgtcgag aatgaggag cttttgaat 
      gttgtaaat ggggaagac gccgacagc tccgactcc 
      gatggcgaa attgaagaa agcgacgaa gatttgaac 
      gactccgat gatgaaatt gaagaaagc gatgaagac 
      gattcaaac accaccgat gaagaaagc gacgaaaac 
      gagctggag aatgaagaa aacgccgcc gaagacacc 
      gccaaccca gcaagagac gaatactcc tcgcacgac 
      acctccgac gaggaggac atccgcacc accgtgggc 
      aacatcccc atgaactgg tacgacgac ttcaaacac 
      ctgggctac gactgggac ggcaaccag atcatcaag 
      cccgagtcg ggcgacctc ctcgacacc ttcctcaag 
      cgcatggaa gaccccgac ttctggcgc accgtcaaa 
      gacccgcaa accggccaa aacgtcgtc ctctccgac 
      gccgacatc gacctcatc aagcgcctc aaagccaac 
      caaatcccg gacgccacc ttcgacgaa tacgccccg 
      tggatcgaa tggttcacg tccgaggtg atgaaaaca 
      ccgctccgg aaattcccc gaacacaag cgctccttc 
      ctgccgagc aaaagcgac gccaagaaa gtctccaag 
      ctggtgcac gccctgaag atgggctgg atcaagacg 
      cgggccgag gccgaaaaa ctgcgcgcg aagaaagag 
      cccgagttt tacatgttg tgggccacc gatgaccag 
      gccgaagac atgaggcgc attcacaaa cacataccg 
      gcaccgaag cgcctcctg cctggccat gccgagtca 
      tataaccca ccaccggag tatttattc gatgaaaaa 
      gaactcaaa cagtggaat aaacataaa aacgccccc 
      tggaaacgg aaattacat ttcgtgcca caaaagttc 
      gagtcactg agacacgtt ccagcgtac cctcgatac 
      ataaaagag aggtttttg cgctgtttg gacttgtat 
      ttgtgcccc cgtgggatt aaaatgaaa ctgaacatc 
      gagcctgaa gctttggtg cccaaactc ccgagtccg 
      aaagaccta caaccgttc cccaccact ctctcgatt 
      gaatacaag ggacatggc gacatggtc cgtaccata 
      agtaccgat aaaatcggc cagtaccta gtgagcggt 
      tccgatgat ggtaccatc aaattatgg gaggttaat 
      accgggcgt tgcctcaaa acgattgac acggggggc 
      gtggtccgt tcagtcgag tggtgccct aaccccgcc 
      ctttccttg atccttgtc gccgcctcc aaccgtgtc 
      ctgctaatc aacccgcac cttggcgac actttgatc 
      acttccaag acggattcg gtcctcgaa accgccccc 
      caaagcgac gcaatcgtg agcgaacga atcaaaacc 
      accgtcgaa tggtcggaa tcaatcgac ccgaaagag 
      tacactcaa ggcgtccga atcgccctc aaacacttc 
      aaggaagtg gcccaggtc acgtggcac ggcaagggc 
      gactacttc gccactgtg atgcccgac ggcctcaac 
      cgctcggtt ctaatcagt cagatcagc cgtcgcagg 
      tcgcaaatc cccttcacc aagtccaaa ggcctagtc 
      cagtgtgtt ttattccac ccgaccaaa ccctgcctc 
      tttgtggcc acgcagcga cacgtccgc atctacgac 
      ctggtgaag caaaccttg atgaagaag ctgctgacg 
      aactccaag tggatttcc accatggcc atacaccca 
      ggcggtgat aatcttcta gtggggacg tacgatcgc 
      aaaatgttg tggtttgac ctggatttg tccaccaag 
      ccgtaccag accttgagg cttcacggg acggcggtc 
      cgcggggtg gccttccat aagcggtac ccgcttttc 
      gcctccggg tccgatgat aagagcttg attgtgtgt 
      cacgggatg gtgtacaac gacttgctg aaaaatcca 
      ctgattgtg cccctgaaa cggctgcag gatcacgaa 
      acgaacaac gattttggc atctttggg gtgcttttc 
      cacccgttg caaccgtgg gtgttctcc tcgggggcc 
      gacgggacc atcaagttg tattctaat taa
    mRNA sequence
    • >TC015584 RA
      ttccgcttc tataaaagt gttattcta gggacatgt 
      ggtaactta acccaactt tcttagcat gtgtggccg 
      ataaacaaa cgtaaaaat gggcaaaaa agaacagtt 
      tttaaaacg aaaattaga aaaatcggc cgaggaaag 
      tgacgagcc tgattctgg tgcagagtc tgtcgagaa 
      tgaggagct tttgaatgt tgtaaatgg ggaagacgc 
      cgacagctc cgactccga tggcgaaat tgaagaaag 
      cgacgaaga tttgaacga ctccgatga tgaaattga 
      agaaagcga tgaagacga ttcaaacac caccgatga 
      agaaagcga cgaaaacga gctggagaa tgaagaaaa 
      cgccgccga agacaccgc caacccagc aagagacga 
      atactcctc gcacgacac ctccgacga ggaggacat 
      ccgcaccac cgtgggcaa catccccat gaactggta 
      cgacgactt caaacacct gggctacga ctgggacgg 
      caaccagat catcaagcc cgagtcggg cgacctcct 
      cgacacctt cctcaagcg catggaaga ccccgactt 
      ctggcgcac cgtcaaaga cccgcaaac cggccaaaa 
      cgtcgtcct ctccgacgc cgacatcga cctcatcaa 
      gcgcctcaa agccaacca aatcccgga cgccacctt 
      cgacgaata cgccccgtg gatcgaatg gttcacgtc 
      cgaggtgat gaaaacacc gctccggaa attccccga 
      acacaagcg ctccttcct gccgagcaa aagcgacgc 
      caagaaagt ctccaagct ggtgcacgc cctgaagat 
      gggctggat caagacgcg ggccgaggc cgaaaaact 
      gcgcgcgaa gaaagagcc cgagtttta catgttgtg 
      ggccaccga tgaccaggc cgaagacat gaggcgcat 
      tcacaaaca cataccggc accgaagcg cctcctgcc 
      tggccatgc cgagtcata taacccacc accggagta 
      tttattcga tgaaaaaga actcaaaca gtggaataa 
      acataaaaa cgccccctg gaaacggaa attacattt 
      cgtgccaca aaagttcga gtcactgag acacgttcc 
      agcgtaccc tcgatacat aaaagagag gtttttgcg 
      ctgtttgga cttgtattt gtgcccccg tgggattaa 
      aatgaaact gaacatcga gcctgaagc tttggtgcc 
      caaactccc gagtccgaa agacctaca accgttccc 
      caccactct ctcgattga atacaaggg acatggcga 
      catggtccg taccataag taccgataa aatcggcca 
      gtacctagt gagcggttc cgatgatgg taccatcaa 
      attatggga ggttaatac cgggcgttg cctcaaaac 
      gattgacac ggggggcgt ggtccgttc agtcgagtg 
      gtgccctaa ccccgccct ttccttgat ccttgtcgc 
      cgcctccaa ccgtgtcct gctaatcaa cccgcacct 
      tggcgacac tttgatcac ttccaagac ggattcggt 
      cctcgaaac cgcccccca aagcgacgc aatcgtgag 
      cgaacgaat caaaaccac cgtcgaatg gtcggaatc 
      aatcgaccc gaaagagta cactcaagg cgtccgaat 
      cgccctcaa acacttcaa ggaagtggc ccaggtcac 
      gtggcacgg caagggcga ctacttcgc cactgtgat 
      gcccgacgg cctcaaccg ctcggttct aatcagtca 
      gatcagccg tcgcaggtc gcaaatccc cttcaccaa 
      gtccaaagg cctagtcca gtgtgtttt attccaccc 
      gaccaaacc ctgcctctt tgtggccac gcagcgaca 
      cgtccgcat ctacgacct ggtgaagca aaccttgat 
      gaagaagct gctgacgaa ctccaagtg gatttccac 
      catggccat acacccagg cggtgataa tcttctagt 
      ggggacgta cgatcgcaa aatgttgtg gtttgacct 
      ggatttgtc caccaagcc gtaccagac cttgaggct 
      tcacgggac ggcggtccg cggggtggc cttccataa 
      gcggtaccc gcttttcgc ctccgggtc cgatgataa 
      gagcttgat tgtgtgtca cgggatggt gtacaacga 
      cttgctgaa aaatccact gattgtgcc cctgaaacg 
      gctgcagga tcacgaaac gaacaacga ttttggcat 
      ctttggggt gcttttcca cccgttgca accgtgggt 
      gttctcctc gggggccga cgggaccat caagttgta 
      ttctaatta ataaatttt tacggaaaa tgttgtgtt 
      gtttggaaa tgcgggaca ggttgagga ggaagattg 
      ccaagaaaa tgttcggaa gttggaaga agacaggtc 
      gagacaag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY