Status
Please wait ...
Gene information
General information
TC034734 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid40: 1558565..1578162
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC034734
    iBeetle screen iB_02408
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    Fertility
    fertility after larval injection decreased (no living offspring) -
    iBeetle screen iB_02493
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 4
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    about 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
    appendages sometimes partially remaining - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva dorsal not closed - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva random partiallynot present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_02408
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: % (includes death as pupa, adult)

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034734 RA
      MVIVEGFSP TRNFFSDFH GLQRLNVPP SRPDLPAKQ 
RLARLYRYF KYEAPGPGL RCVVYLERV KRVFRAHLR 
EVVSEVMLE DVVTMCLLQ ALKDYERDK SEVRGVLRK 
LPQRQLKTV KDELEYVCD NLPGDFFKK GSEMARLIK 
SDVEKPAEK PLDIIFYIP MSENEPCIE GEVEELSLA 
AAASTGAPY CVKKRYDYY KSKMAEFPF EEFKEIIIT 
GITSQKSNE ELLNYFLDV FGCEVFEFL NEIIQHRSA 
QIEWGDGEK PAPTQRRLE PKVNRYTPA LASQVIVQS 
EEEKNLAKT LRKIERKQK GGPQPDFLN ELEGDLNYI 
KHQPIFRKG PQTVDYPHV HDQLRNISI TTKFNGVTL 
KQPENTVKK ETSTHVEFT LPGGKRPKN DDIDLVKVT 
SLDPTGRLV FKDIKEFNR IQSEVFPVA YNTNENMLI 
CAPTGAGKT NIALLAIVH QIKAHMEGG LIRKDDFKI 
VYVCPMKAL ATEMVSNFS KKLAPVGIV VKELTGDMQ 
LTKKEIAET QMLVTTPEK WDVISRKGA VDTEVTSLV 
KLLILDEVH LLNSDRGPV IEALVARTL RQVLSSQSI 
IRIVALSAT LPGYLDVAN FLKVNPNTG LFFFDNRFR 
SVPLTMTFI GVKNKNDQD AMDLICYNK IIPIIKDGQ 
QVMVFVTSR NLTAVVAKN LLTHAKNNN VLANFIPDK 
KHRIGKNFK SSELELLVP NGFGVHHAG MCRSDRLEV 
ESLFRVGAL KVIVCTTTL AWGVNLPAH AVIIRGTTR 
YDAQKSSYV DMDMLDIQQ IFGRAGRPQ YDTSGHGMI 
ITSVQNMAN YMSLLTSQA PIESQFLNN VPDHLNAEI 
VLGTVSNLK EAMEWLTNT FVYCRIKKN PLVYGLTFT 
EIWEPEKLF QYLERKLFD AASTLESAQ MVRFNPTLG 
ELRPTNYGR IASFYYISH QTMKYFHDH FERSMVEAD 
ILHLISNAS EFQHIQVRN DELDELDRL HEEFSQFEF 
NLDPSAVVF KVLVLIQAN ISRAKIRVS SLVSDCEFI 
MQSVTRLAR ALFEIAVDK NYALQVWRC LEVARMVEQ 
QAWTDRHPL MQFKELEIK GHRALDVLH NIPIEELQE 
MTEREILDL VRSRHLASR VHHFCKAFP RVNLDVSVK 
PITEGVIRL QLLIGANFS WDSSIHGNV QHYYAWVED 
PTHDSIYHF ESFIITKKQ VISKEPIEL IFTVPLQKP 
HSNEYFVTV VNSKYMAAE TSYRIDLDK LHLLPSYSI 
QTKFLSVRP LPKTALHNT EFENLYSFT HFNAVQSQV 
FHCCFNTDS NVLLGAPTG SGKTIVSEI CILRLFANR 
PERKVVYIA PMKALVRER VLDWTPKFA KIGKKVVEV 
TGDVTPHSS LISTSHIII TTPEKWDGM SRNWLQKDF 
VKQVGLIII DEIHLLAED RGPVLEVIV SRMNYINSI 
KNAKVRIVG LSTAMANAG DLANWLNIE KKGLYNFSS 
SVRPVPLEI HLKGFSAKN YCPRMATMN RPAYQAICQ 
YAPESPTLI FVSSRKQTR ITSYDLIKC LLSDTNPKQ 
WLHCQQDEI EQVRKTITD SDLSYLLLF GIGIHHAGL 
QDHDRKTVE ELFVTQKIQ VLVATATLA WGVNFPAHL 
VIVKGTEYY DGATKRYVD MPVTDVLQM MGRAGRPQF 
DTSGVACVF VQESKKNFY RKFLFEPFP VESNLLQVL 
PEHVNAEIA NGTVTCRSQ LVDLICSTF FFRRLLVNP 
SYYKMEGGD ANQFLNELA DSVAGQLQE TQCIVIRDE 
DVQDFYEST FLGQIAAQY YLSCKTVLN LDQNMSRDS 
SFDDLLAHM CHVEEYALF PVRHNEDKI NRHLTLDLN 
VKTRQPFDS PHLKVLLLI KCYLSDKKL PNQEYVVDL 
KTVFDQVIR IMQAMISLT SHKGWLDCT LKLIYLGQM 
LIQGLMIDV GSVMMLPHV TPETRDRLS RHLAVANIR 
VPHLKSLLR QDRKSTTRL LDEHFGEKS NEILKILAR 
LPTLEIKFT VKNGETNGN IDYRLGDNI VTFEAKART 
TCLFEFEVL RDGTNNLSV YSRKFSKQK DESWFFVMA 
FEDDLVRLQ RFAIKKSKS LEVEVQVPG ERGVYEYAG 
YFMSDSYIG LDQKVTFKV TVVD
    CDS sequence
    • >TC034734 RA
      atggttatt gttgagggt ttttcaccc acccgtaac 
ttcttcagc gacttccac ggcctgcaa cgcctcaat 
gtgcccccc agtcgcccc gatttgccc gcaaagcaa 
agactggcc cgcttgtac cgttacttc aagtatgag 
gccccggga cccgggctg aggtgcgtc gtgtacctg 
gagcgcgtc aaacgtgtc tttagggca catttacgc 
gaagttgtg agtgaggtt atgttggag gacgtggtc 
actatgtgt ctgctccaa gcgttgaaa gactacgag 
agggacaag agtgaggtc aggggcgtt ttgaggaag 
ttgccccaa agacaatta aagacagtc aaggatgag 
ttggagtac gtttgtgat aatctcccg ggtgatttt 
ttcaaaaag gggtcagag atggcacgg ttgataaaa 
tcggacgtt gaaaaaccg gccgagaag ccacttgat 
ataattttt tacattccg atgagtgag aatgagccc 
tgtattgaa ggggaggtt gaggagttg agtcttgcg 
gccgccgcc tcaacaggg gccccttac tgcgttaaa 
aaacgctac gattattat aagagcaaa atggcggag 
tttccgttt gaggaattc aaggaaatc atcataact 
ggaataacc tcacaaaaa tcaaacgag gaactcctc 
aattatttc ttggacgtt ttcgggtgc gaagttttc 
gagtttttg aacgaaatt atccagcac agaagtgca 
caaattgag tggggcgac ggggaaaaa ccggccccc 
actcagcga agactcgaa ccaaaagtc aaccgttac 
acgcctgcc ttagccagt caagtgatc gtccagagc 
gaggaagag aaaaactta gccaaaaca ttacgcaaa 
atcgaacgc aagcaaaaa gggggtccc caacccgac 
tttttaaac gagcttgag ggcgactta aactacatc 
aagcaccag cccattttc agaaagggg ccccaaact 
gtggactat cctcatgtt cacgaccag ttgcgcaat 
atttccatt acgacgaaa tttaacggg gttacgttg 
aaacagccc gaaaatacg gttaaaaag gagacttcg 
acgcatgtt gagtttacg ctaccgggg ggcaaaagg 
ccaaaaaat gatgatata gatttggtg aaagtcact 
agtttggac ccgactggg cgtttggtg tttaaggat 
ataaaggag tttaaccgg atacagtcg gaggtgttt 
ccggtggcg tacaatacg aatgaaaat atgctgatt 
tgtgccccc acgggggcg gggaagact aatatagcg 
ctgctggcg atcgtgcac cagattaag gcgcatatg 
gaagggggg ctgatccgc aaggatgat ttcaagatt 
gtgtatgtg tgccccatg aaagcactc gccaccgaa 
atggtctca aactttagc aaaaaactc gcccctgtc 
ggtatagta gtgaaagaa ctgaccggc gacatgcaa 
ctaaccaaa aaagaaatc gccgaaacc caaatgttg 
gtcacaacg cccgaaaaa tgggacgtc atctcgcgc 
aagggggct gtggacacc gaagtcaca agcctcgtt 
aaattacta atcctcgac gaagtccat ttattgaac 
agtgacagg ggccccgtt attgaagcc ctagtcgcg 
cgcaccctg cgccaggtc ctcagcagt caaagcatc 
atccgaatt gtggccctt tcggccacc ctgcccggc 
tacctcgac gttgccaat ttcctcaaa gtcaaccct 
aatacgggc ctgtttttc ttcgataat cgttttcgt 
tccgtcccc ctgaccatg accttcatt ggggttaaa 
aataaaaat gatcaagac gccatggac ctcatttgt 
tacaacaaa atcatcccg attatcaaa gatggccaa 
caagttatg gtgtttgtg acgtcacgc aacttgaca 
gctgttgta gcgaagaat ttgctcaca catgcgaaa 
aataacaac gttttggcg aattttatc cccgataag 
aagcacaga attgggaag aattttaaa agtagtgag 
ttggaattg ttggttccg aatggtttt ggggtccac 
catgctggc atgtgtcgg agtgatcgc ttggaagtt 
gagagttta ttccgtgtg ggggcgctt aaagtaata 
gtgtgtact actaccctt gcgtggggg gtgaatttg 
cccgcgcat gcggtcata atcaggggc acgactcga 
tacgacgcc caaaagtcg tcatacgtt gatatggac 
atgttggac atacagcag attttcggg cgtgccgga 
aggccccag tatgacacg tccggtcat ggtatgatt 
atcacaagt gtgcaaaat atggccaat tatatgtcg 
cttctgacg tcacaagca ccaatcgaa agccagttt 
ttgaacaac gttcctgat catttgaac gccgagatt 
gtgctagga acggtgtcg aatttaaag gaggccatg 
gagtggctg accaatact tttgtttat tgcaggatt 
aagaaaaat ccactggtt tatggcctg actttcacc 
gagatttgg gagcctgaa aagctcttt cagtacctg 
gagcgcaaa ctcttcgac gccgcctcc acgctggaa 
tcggcccaa atggtccgt ttcaacccg actttgggc 
gaactccgt ccgacaaac tacggccgt atcgccagt 
ttttactac atctcgcac caaacaatg aaatatttc 
cacgaccat ttcgaacgt tcaatggtc gaagccgac 
atcctccac ctcatcagc aacgcatcc gaattccag 
cacatccaa gtccggaac gacgagctc gacgagctc 
gatcgcctc cacgaagag ttcagtcaa ttcgaattc 
aacctagac ccgagcgct gttgtcttc aaagttttg 
gttctgatc caggctaac atatcacgt gccaaaatc 
cgcgtctcg tcattggtc tccgactgc gagttcata 
atgcaaagt gtgacgcga ttggcgcga gcgctattc 
gaaattgcg gttgataaa aattacgct ttgcaagtg 
tggcgctgc ttggaggtg gcacgaatg gttgaacag 
caagcttgg actgacagg catcctttg atgcagttc 
aaggagttg gagattaag gggcacagg gcacttgat 
gtgcttcat aatataccg attgaagaa ttacaagag 
atgacagag cgggaaatt ttggatttg gttagaagt 
agacatttg gcgagtcgg gttcaccat ttttgcaaa 
gcttttcca cgtgttaac cttgacgtt tcggttaaa 
ccgatcaca gagggcgtg atcaggtta cagttgctg 
attggtgct aacttcagt tgggatagt agtatccat 
ggtaacgtg cagcattac tacgcgtgg gtggaggac 
cccacacat gatagtatc tatcatttt gaatcgttt 
attatcacg aaaaaacaa gtgataagt aaggaacca 
atcgaattg atttttacg gtgccgttg caaaaaccg 
cactcaaat gaatatttt gtgacggtt gttaatagc 
aaatatatg gccgcggaa acgtcctac agaatcgac 
ttggacaag ttacacctt ttgccaagc tactcaatc 
cagacgaaa tttttaagc gtgcgacct ttacccaaa 
acggcctta cataacacc gagtttgaa aatttgtac 
agttttaca cactttaac gccgtacaa agccaagtg 
tttcattgc tgtttcaac accgactcc aatgtttta 
ctcggagca ccgacaggt tccgggaag acaatagtg 
tcggaaatt tgtattttg cgattattt gcgaaccga 
cccgagcga aaagttgtc tatattgca ccaatgaag 
gctctagtt cgcgagcgt gtccttgat tggacgcca 
aaattcgcc aaaattggt aaaaaagtg gtggaagtg 
accggtgat gtcacccct cattcttca ctaatctcg 
acgtctcat attattatt acaacgccg gaaaagtgg 
gatggaatg agtcgaaat tggcttcag aaggatttt 
gttaaacaa gtcggactt attataatt gacgaaatt 
catcttttg gctgaagat cgaggtcca gtgcttgaa 
gttatcgtt tccaggatg aactacatc aacagtatc 
aagaatgct aaggtccga atcgtcggc ctttcaacc 
gccatggcc aacgctggc gatttagcc aactggttg 
aacatcgaa aaaaaggga ctttacaat tttagttca 
tccgtacgt cccgtcccc cttgaaata cacctgaag 
ggtttttcg gccaaaaac tactgtccc cgaatggct 
acaatgaac cgaccagct tatcaggcc atatgtcaa 
tacgccccc gaaagtccg accctgata ttcgtctcg 
tcccgaaaa caaacgcgc atcacctcc tacgaccta 
atcaagtgt cttttaagc gacactaac ccgaaacaa 
tggctccac tgccaacag gacgagatc gaacaagtc 
cgaaaaact ataaccgat tcggaccta agttaccta 
cttttgttc ggaatcgga atacatcac gcaggcctt 
caagaccac gaccgcaaa acagtcgaa gaactgttt 
gtcacgcaa aaaatccag gtgcttgtg gccacggcc 
accctagca tggggcgtt aatttccca gcccacttg 
gttatagtt aaagggacc gaatattac gacggtgcg 
actaaacga tacgttgat atgccggtc accgacgtt 
ttacaaatg atgggacgg gctggacgt ccacaattc 
gataccagt ggcgtagcg tgtgttttt gtccaagag 
agtaaaaaa aatttctat cggaaattt ttattcgaa 
ccgtttccg gtcgaatca aacttgttg caagtttta 
cctgaacat gtgaatgcg gaaattgca aatggcacg 
gtcacgtgt cgctcacaa ttagtcgat ttaatttgt 
tcgacgttt ttcttccga aggctattg gttaacccg 
agttattat aaaatggaa ggtggggat gcgaatcaa 
tttttgaat gaattggcc gattcggtt gccgggcaa 
ttgcaagaa acgcagtgt atagtgatt cgagatgag 
gacgtgcaa gacttttac gaaagcact tttttgggc 
caaatcgca gctcaatac tacctttca tgcaaaact 
gtgctaaat ctggaccaa aacatgtca cgtgactcc 
tcattcgac gatctattg gctcacatg tgtcacgtg 
gaagagtac gccctcttt cccgtacgt cacaacgag 
gataaaatc aaccgtcat ttgacgctt gatttaaat 
gtcaaaacg cgccaaccg ttcgattca cctcatttg 
aaggtttta ctccttatc aagtgttac ctcagtgat 
aaaaaacta ccaaatcaa gaatatgtc gttgatttg 
aaaactgtt tttgaccaa gtcattcgg atcatgcag 
gccatgatc agtctgacg tcacacaaa ggctggctt 
gattgcacc ctcaaattg atttatttg gggcaaatg 
ttgatccag ggattgatg atagatgta ggtagtgtg 
atgatgctg cctcacgtg acgcccgag acacgtgac 
cggctttcg cgccatctg gcagtggct aacatcaga 
gtgccccac cttaagtca cttttgcgc caagatcgc 
aaatcaacg actcggctt ttggatgaa catttcggc 
gagaaatcg aatgaaatt ttaaagatt ttggcacgt 
ctaccaaca cttgaaatt aaatttact gtgaaaaat 
ggcgaaacg aatggcaac attgactat agacttggg 
gacaacatt gtcactttt gaggctaag gctagaacg 
acgtgtttg ttcgagttt gaggttttg agggacggg 
accaacaat ttgagtgtt tatagccga aagtttagt 
aaacagaag gatgagtct tggtttttt gtcatggct 
tttgaggac gatttggtg aggttgcag cggtttgcc 
atcaagaag agtaaaagt ttggaggtt gaagttcaa 
gtgccgggg gagcgaggg gtttacgag tacgcgggg 
tactttatg agcgattcc tacatcggg ttggaccag 
aaggttacg tttaaggtg accgtggtg gattga
    mRNA sequence
    • >TC034734 RA
      aatgtttcc aataaaaaa catgtaaaa aacaaaaaa 
aatattgtg gtagataat aaaaacaaa cggtttccc 
ctgttattg tactgtgat ttatagttt taagcaatg 
gttattgtt gagggtttt tcacccacc cgtaacttc 
ttcagcgac ttccacggc ctgcaacgc ctcaatgtg 
ccccccagt cgccccgat ttgcccgca aagcaaaga 
ctggcccgc ttgtaccgt tacttcaag tatgaggcc 
ccgggaccc gggctgagg tgcgtcgtg tacctggag 
cgcgtcaaa cgtgtcttt agggcacat ttacgcgaa 
gttgtgagt gaggttatg ttggaggac gtggtcact 
atgtgtctg ctccaagcg ttgaaagac tacgagagg 
gacaagagt gaggtcagg ggcgttttg aggaagttg 
ccccaaaga caattaaag acagtcaag gatgagttg 
gagtacgtt tgtgataat ctcccgggt gattttttc 
aaaaagggg tcagagatg gcacggttg ataaaatcg 
gacgttgaa aaaccggcc gagaagcca cttgatata 
attttttac attccgatg agtgagaat gagccctgt 
attgaaggg gaggttgag gagttgagt cttgcggcc 
gccgcctca acaggggcc ccttactgc gttaaaaaa 
cgctacgat tattataag agcaaaatg gcggagttt 
ccgtttgag gaattcaag gaaatcatc ataactgga 
ataacctca caaaaatca aacgaggaa ctcctcaat 
tatttcttg gacgttttc gggtgcgaa gttttcgag 
tttttgaac gaaattatc cagcacaga agtgcacaa 
attgagtgg ggcgacggg gaaaaaccg gcccccact 
cagcgaaga ctcgaacca aaagtcaac cgttacacg 
cctgcctta gccagtcaa gtgatcgtc cagagcgag 
gaagagaaa aacttagcc aaaacatta cgcaaaatc 
gaacgcaag caaaaaggg ggtccccaa cccgacttt 
ttaaacgag cttgagggc gacttaaac tacatcaag 
caccagccc attttcaga aaggggccc caaactgtg 
gactatcct catgttcac gaccagttg cgcaatatt 
tccattacg acgaaattt aacggggtt acgttgaaa 
cagcccgaa aatacggtt aaaaaggag acttcgacg 
catgttgag tttacgcta ccggggggc aaaaggcca 
aaaaatgat gatatagat ttggtgaaa gtcactagt 
ttggacccg actgggcgt ttggtgttt aaggatata 
aaggagttt aaccggata cagtcggag gtgtttccg 
gtggcgtac aatacgaat gaaaatatg ctgatttgt 
gcccccacg ggggcgggg aagactaat atagcgctg 
ctggcgatc gtgcaccag attaaggcg catatggaa 
ggggggctg atccgcaag gatgatttc aagattgtg 
tatgtgtgc cccatgaaa gcactcgcc accgaaatg 
gtctcaaac tttagcaaa aaactcgcc cctgtcggt 
atagtagtg aaagaactg accggcgac atgcaacta 
accaaaaaa gaaatcgcc gaaacccaa atgttggtc 
acaacgccc gaaaaatgg gacgtcatc tcgcgcaag 
ggggctgtg gacaccgaa gtcacaagc ctcgttaaa 
ttactaatc ctcgacgaa gtccattta ttgaacagt 
gacaggggc cccgttatt gaagcccta gtcgcgcgc 
accctgcgc caggtcctc agcagtcaa agcatcatc 
cgaattgtg gccctttcg gccaccctg cccggctac 
ctcgacgtt gccaatttc ctcaaagtc aaccctaat 
acgggcctg tttttcttc gataatcgt tttcgttcc 
gtccccctg accatgacc ttcattggg gttaaaaat 
aaaaatgat caagacgcc atggacctc atttgttac 
aacaaaatc atcccgatt atcaaagat ggccaacaa 
gttatggtg tttgtgacg tcacgcaac ttgacagct 
gttgtagcg aagaatttg ctcacacat gcgaaaaat 
aacaacgtt ttggcgaat tttatcccc gataagaag 
cacagaatt gggaagaat tttaaaagt agtgagttg 
gaattgttg gttccgaat ggttttggg gtccaccat 
gctggcatg tgtcggagt gatcgcttg gaagttgag 
agtttattc cgtgtgggg gcgcttaaa gtaatagtg 
tgtactact acccttgcg tggggggtg aatttgccc 
gcgcatgcg gtcataatc aggggcacg actcgatac 
gacgcccaa aagtcgtca tacgttgat atggacatg 
ttggacata cagcagatt ttcgggcgt gccggaagg 
ccccagtat gacacgtcc ggtcatggt atgattatc 
acaagtgtg caaaatatg gccaattat atgtcgctt 
ctgacgtca caagcacca atcgaaagc cagtttttg 
aacaacgtt cctgatcat ttgaacgcc gagattgtg 
ctaggaacg gtgtcgaat ttaaaggag gccatggag 
tggctgacc aatactttt gtttattgc aggattaag 
aaaaatcca ctggtttat ggcctgact ttcaccgag 
atttgggag cctgaaaag ctctttcag tacctggag 
cgcaaactc ttcgacgcc gcctccacg ctggaatcg 
gcccaaatg gtccgtttc aacccgact ttgggcgaa 
ctccgtccg acaaactac ggccgtatc gccagtttt 
tactacatc tcgcaccaa acaatgaaa tatttccac 
gaccatttc gaacgttca atggtcgaa gccgacatc 
ctccacctc atcagcaac gcatccgaa ttccagcac 
atccaagtc cggaacgac gagctcgac gagctcgat 
cgcctccac gaagagttc agtcaattc gaattcaac 
ctagacccg agcgctgtt gtcttcaaa gttttggtt 
ctgatccag gctaacata tcacgtgcc aaaatccgc 
gtctcgtca ttggtctcc gactgcgag ttcataatg 
caaagtgtg acgcgattg gcgcgagcg ctattcgaa 
attgcggtt gataaaaat tacgctttg caagtgtgg 
cgctgcttg gaggtggca cgaatggtt gaacagcaa 
gcttggact gacaggcat cctttgatg cagttcaag 
gagttggag attaagggg cacagggca cttgatgtg 
cttcataat ataccgatt gaagaatta caagagatg 
acagagcgg gaaattttg gatttggtt agaagtaga 
catttggcg agtcgggtt caccatttt tgcaaagct 
tttccacgt gttaacctt gacgtttcg gttaaaccg 
atcacagag ggcgtgatc aggttacag ttgctgatt 
ggtgctaac ttcagttgg gatagtagt atccatggt 
aacgtgcag cattactac gcgtgggtg gaggacccc 
acacatgat agtatctat cattttgaa tcgtttatt 
atcacgaaa aaacaagtg ataagtaag gaaccaatc 
gaattgatt tttacggtg ccgttgcaa aaaccgcac 
tcaaatgaa tattttgtg acggttgtt aatagcaaa 
tatatggcc gcggaaacg tcctacaga atcgacttg 
gacaagtta caccttttg ccaagctac tcaatccag 
acgaaattt ttaagcgtg cgaccttta cccaaaacg 
gccttacat aacaccgag tttgaaaat ttgtacagt 
tttacacac tttaacgcc gtacaaagc caagtgttt 
cattgctgt ttcaacacc gactccaat gttttactc 
ggagcaccg acaggttcc gggaagaca atagtgtcg 
gaaatttgt attttgcga ttatttgcg aaccgaccc 
gagcgaaaa gttgtctat attgcacca atgaaggct 
ctagttcgc gagcgtgtc cttgattgg acgccaaaa 
ttcgccaaa attggtaaa aaagtggtg gaagtgacc 
ggtgatgtc acccctcat tcttcacta atctcgacg 
tctcatatt attattaca acgccggaa aagtgggat 
ggaatgagt cgaaattgg cttcagaag gattttgtt 
aaacaagtc ggacttatt ataattgac gaaattcat 
cttttggct gaagatcga ggtccagtg cttgaagtt 
atcgtttcc aggatgaac tacatcaac agtatcaag 
aatgctaag gtccgaatc gtcggcctt tcaaccgcc 
atggccaac gctggcgat ttagccaac tggttgaac 
atcgaaaaa aagggactt tacaatttt agttcatcc 
gtacgtccc gtccccctt gaaatacac ctgaagggt 
ttttcggcc aaaaactac tgtccccga atggctaca 
atgaaccga ccagcttat caggccata tgtcaatac 
gcccccgaa agtccgacc ctgatattc gtctcgtcc 
cgaaaacaa acgcgcatc acctcctac gacctaatc 
aagtgtctt ttaagcgac actaacccg aaacaatgg 
ctccactgc caacaggac gagatcgaa caagtccga 
aaaactata accgattcg gacctaagt tacctactt 
ttgttcgga atcggaata catcacgca ggccttcaa 
gaccacgac cgcaaaaca gtcgaagaa ctgtttgtc 
acgcaaaaa atccaggtg cttgtggcc acggccacc 
ctagcatgg ggcgttaat ttcccagcc cacttggtt 
atagttaaa gggaccgaa tattacgac ggtgcgact 
aaacgatac gttgatatg ccggtcacc gacgtttta 
caaatgatg ggacgggct ggacgtcca caattcgat 
accagtggc gtagcgtgt gtttttgtc caagagagt 
aaaaaaaat ttctatcgg aaattttta ttcgaaccg 
tttccggtc gaatcaaac ttgttgcaa gttttacct 
gaacatgtg aatgcggaa attgcaaat ggcacggtc 
acgtgtcgc tcacaatta gtcgattta atttgttcg 
acgtttttc ttccgaagg ctattggtt aacccgagt 
tattataaa atggaaggt ggggatgcg aatcaattt 
ttgaatgaa ttggccgat tcggttgcc gggcaattg 
caagaaacg cagtgtata gtgattcga gatgaggac 
gtgcaagac ttttacgaa agcactttt ttgggccaa 
atcgcagct caatactac ctttcatgc aaaactgtg 
ctaaatctg gaccaaaac atgtcacgt gactcctca 
ttcgacgat ctattggct cacatgtgt cacgtggaa 
gagtacgcc ctctttccc gtacgtcac aacgaggat 
aaaatcaac cgtcatttg acgcttgat ttaaatgtc 
aaaacgcgc caaccgttc gattcacct catttgaag 
gttttactc cttatcaag tgttacctc agtgataaa 
aaactacca aatcaagaa tatgtcgtt gatttgaaa 
actgttttt gaccaagtc attcggatc atgcaggcc 
atgatcagt ctgacgtca cacaaaggc tggcttgat 
tgcaccctc aaattgatt tatttgggg caaatgttg 
atccaggga ttgatgata gatgtaggt agtgtgatg 
atgctgcct cacgtgacg cccgagaca cgtgaccgg 
ctttcgcgc catctggca gtggctaac atcagagtg 
ccccacctt aagtcactt ttgcgccaa gatcgcaaa 
tcaacgact cggcttttg gatgaacat ttcggcgag 
aaatcgaat gaaatttta aagattttg gcacgtcta 
ccaacactt gaaattaaa tttactgtg aaaaatggc 
gaaacgaat ggcaacatt gactataga cttggggac 
aacattgtc acttttgag gctaaggct agaacgacg 
tgtttgttc gagtttgag gttttgagg gacgggacc 
aacaatttg agtgtttat agccgaaag tttagtaaa 
cagaaggat gagtcttgg ttttttgtc atggctttt 
gaggacgat ttggtgagg ttgcagcgg tttgccatc 
aagaagagt aaaagtttg gaggttgaa gttcaagtg 
ccgggggag cgaggggtt tacgagtac gcggggtac 
tttatgagc gattcctac atcgggttg gaccagaag 
gttacgttt aaggtgacc gtggtggat tgagtggat 
gtactagtg gagttatga aattttaaa tgaattgta 
tttatgttt gtatttttc tgtgtaaat aaaaattta 
ctttataa