General information | |||
TC034734 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007421.3: 2514789..2534386 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC034734 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
Gene | {{ goGene }} |
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GO ID | * {{ blurIdResult }} |
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Evidence | {{ blurEvidenceResult }} |
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Reference | * {{ blurReferenceResult }} |
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Quotation | ||
Name | ||
Publish name | ||
{{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_02408 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (11 dpi)embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_02493 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
remarks: muscle pattern potentially obscured by segmentation defect,
about 50 |
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
appendages sometimes partially remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%
larva dorsal not closed - Penetrance (percentage of animals/eggs on slide): less than 30%
larva random partiallynot present - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_02408 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: % (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC034734 RA
MVIVEGFSP TRNFFSDFH GLQRLNVPP SRPDLPAKQ RLARLYRYF KYEAPGPGL RCVVYLERV KRVFRAHLR EVVSEVMLE DVVTMCLLQ ALKDYERDK SEVRGVLRK LPQRQLKTV KDELEYVCD NLPGDFFKK GSEMARLIK SDVEKPAEK PLDIIFYIP MSENEPCIE GEVEELSLA AAASTGAPY CVKKRYDYY KSKMAEFPF EEFKEIIIT GITSQKSNE ELLNYFLDV FGCEVFEFL NEIIQHRSA QIEWGDGEK PAPTQRRLE PKVNRYTPA LASQVIVQS EEEKNLAKT LRKIERKQK GGPQPDFLN ELEGDLNYI KHQPIFRKG PQTVDYPHV HDQLRNISI TTKFNGVTL KQPENTVKK ETSTHVEFT LPGGKRPKN DDIDLVKVT SLDPTGRLV FKDIKEFNR IQSEVFPVA YNTNENMLI CAPTGAGKT NIALLAIVH QIKAHMEGG LIRKDDFKI VYVCPMKAL ATEMVSNFS KKLAPVGIV VKELTGDMQ LTKKEIAET QMLVTTPEK WDVISRKGA VDTEVTSLV KLLILDEVH LLNSDRGPV IEALVARTL RQVLSSQSI IRIVALSAT LPGYLDVAN FLKVNPNTG LFFFDNRFR SVPLTMTFI GVKNKNDQD AMDLICYNK IIPIIKDGQ QVMVFVTSR NLTAVVAKN LLTHAKNNN VLANFIPDK KHRIGKNFK SSELELLVP NGFGVHHAG MCRSDRLEV ESLFRVGAL KVIVCTTTL AWGVNLPAH AVIIRGTTR YDAQKSSYV DMDMLDIQQ IFGRAGRPQ YDTSGHGMI ITSVQNMAN YMSLLTSQA PIESQFLNN VPDHLNAEI VLGTVSNLK EAMEWLTNT FVYCRIKKN PLVYGLTFT EIWEPEKLF QYLERKLFD AASTLESAQ MVRFNPTLG ELRPTNYGR IASFYYISH QTMKYFHDH FERSMVEAD ILHLISNAS EFQHIQVRN DELDELDRL HEEFSQFEF NLDPSAVVF KVLVLIQAN ISRAKIRVS SLVSDCEFI MQSVTRLAR ALFEIAVDK NYALQVWRC LEVARMVEQ QAWTDRHPL MQFKELEIK GHRALDVLH NIPIEELQE MTEREILDL VRSRHLASR VHHFCKAFP RVNLDVSVK PITEGVIRL QLLIGANFS WDSSIHGNV QHYYAWVED PTHDSIYHF ESFIITKKQ VISKEPIEL IFTVPLQKP HSNEYFVTV VNSKYMAAE TSYRIDLDK LHLLPSYSI QTKFLSVRP LPKTALHNT EFENLYSFT HFNAVQSQV FHCCFNTDS NVLLGAPTG SGKTIVSEI CILRLFANR PERKVVYIA PMKALVRER VLDWTPKFA KIGKKVVEV TGDVTPHSS LISTSHIII TTPEKWDGM SRNWLQKDF VKQVGLIII DEIHLLAED RGPVLEVIV SRMNYINSI KNAKVRIVG LSTAMANAG DLANWLNIE KKGLYNFSS SVRPVPLEI HLKGFSAKN YCPRMATMN RPAYQAICQ YAPESPTLI FVSSRKQTR ITSYDLIKC LLSDTNPKQ WLHCQQDEI EQVRKTITD SDLSYLLLF GIGIHHAGL QDHDRKTVE ELFVTQKIQ VLVATATLA WGVNFPAHL VIVKGTEYY DGATKRYVD MPVTDVLQM MGRAGRPQF DTSGVACVF VQESKKNFY RKFLFEPFP VESNLLQVL PEHVNAEIA NGTVTCRSQ LVDLICSTF FFRRLLVNP SYYKMEGGD ANQFLNELA DSVAGQLQE TQCIVIRDE DVQDFYEST FLGQIAAQY YLSCKTVLN LDQNMSRDS SFDDLLAHM CHVEEYALF PVRHNEDKI NRHLTLDLN VKTRQPFDS PHLKVLLLI KCYLSDKKL PNQEYVVDL KTVFDQVIR IMQAMISLT SHKGWLDCT LKLIYLGQM LIQGLMIDV GSVMMLPHV TPETRDRLS RHLAVANIR VPHLKSLLR QDRKSTTRL LDEHFGEKS NEILKILAR LPTLEIKFT VKNGETNGN IDYRLGDNI VTFEAKART TCLFEFEVL RDGTNNLSV YSRKFSKQK DESWFFVMA FEDDLVRLQ RFAIKKSKS LEVEVQVPG ERGVYEYAG YFMSDSYIG LDQKVTFKV TVVD
- >TC034734 RA
atggttatt gttgagggt ttttcaccc acccgtaac ttcttcagc gacttccac ggcctgcaa cgcctcaat gtgcccccc agtcgcccc gatttgccc gcaaagcaa agactggcc cgcttgtac cgttacttc aagtatgag gccccggga cccgggctg aggtgcgtc gtgtacctg gagcgcgtc aaacgtgtc tttagggca catttacgc gaagttgtg agtgaggtt atgttggag gacgtggtc actatgtgt ctgctccaa gcgttgaaa gactacgag agggacaag agtgaggtc aggggcgtt ttgaggaag ttgccccaa agacaatta aagacagtc aaggatgag ttggagtac gtttgtgat aatctcccg ggtgatttt ttcaaaaag gggtcagag atggcacgg ttgataaaa tcggacgtt gaaaaaccg gccgagaag ccacttgat ataattttt tacattccg atgagtgag aatgagccc tgtattgaa ggggaggtt gaggagttg agtcttgcg gccgccgcc tcaacaggg gccccttac tgcgttaaa aaacgctac gattattat aagagcaaa atggcggag tttccgttt gaggaattc aaggaaatc atcataact ggaataacc tcacaaaaa tcaaacgag gaactcctc aattatttc ttggacgtt ttcgggtgc gaagttttc gagtttttg aacgaaatt atccagcac agaagtgca caaattgag tggggcgac ggggaaaaa ccggccccc actcagcga agactcgaa ccaaaagtc aaccgttac acgcctgcc ttagccagt caagtgatc gtccagagc gaggaagag aaaaactta gccaaaaca ttacgcaaa atcgaacgc aagcaaaaa gggggtccc caacccgac tttttaaac gagcttgag ggcgactta aactacatc aagcaccag cccattttc agaaagggg ccccaaact gtggactat cctcatgtt cacgaccag ttgcgcaat atttccatt acgacgaaa tttaacggg gttacgttg aaacagccc gaaaatacg gttaaaaag gagacttcg acgcatgtt gagtttacg ctaccgggg ggcaaaagg ccaaaaaat gatgatata gatttggtg aaagtcact agtttggac ccgactggg cgtttggtg tttaaggat ataaaggag tttaaccgg atacagtcg gaggtgttt ccggtggcg tacaatacg aatgaaaat atgctgatt tgtgccccc acgggggcg gggaagact aatatagcg ctgctggcg atcgtgcac cagattaag gcgcatatg gaagggggg ctgatccgc aaggatgat ttcaagatt gtgtatgtg tgccccatg aaagcactc gccaccgaa atggtctca aactttagc aaaaaactc gcccctgtc ggtatagta gtgaaagaa ctgaccggc gacatgcaa ctaaccaaa aaagaaatc gccgaaacc caaatgttg gtcacaacg cccgaaaaa tgggacgtc atctcgcgc aagggggct gtggacacc gaagtcaca agcctcgtt aaattacta atcctcgac gaagtccat ttattgaac agtgacagg ggccccgtt attgaagcc ctagtcgcg cgcaccctg cgccaggtc ctcagcagt caaagcatc atccgaatt gtggccctt tcggccacc ctgcccggc tacctcgac gttgccaat ttcctcaaa gtcaaccct aatacgggc ctgtttttc ttcgataat cgttttcgt tccgtcccc ctgaccatg accttcatt ggggttaaa aataaaaat gatcaagac gccatggac ctcatttgt tacaacaaa atcatcccg attatcaaa gatggccaa caagttatg gtgtttgtg acgtcacgc aacttgaca gctgttgta gcgaagaat ttgctcaca catgcgaaa aataacaac gttttggcg aattttatc cccgataag aagcacaga attgggaag aattttaaa agtagtgag ttggaattg ttggttccg aatggtttt ggggtccac catgctggc atgtgtcgg agtgatcgc ttggaagtt gagagttta ttccgtgtg ggggcgctt aaagtaata gtgtgtact actaccctt gcgtggggg gtgaatttg cccgcgcat gcggtcata atcaggggc acgactcga tacgacgcc caaaagtcg tcatacgtt gatatggac atgttggac atacagcag attttcggg cgtgccgga aggccccag tatgacacg tccggtcat ggtatgatt atcacaagt gtgcaaaat atggccaat tatatgtcg cttctgacg tcacaagca ccaatcgaa agccagttt ttgaacaac gttcctgat catttgaac gccgagatt gtgctagga acggtgtcg aatttaaag gaggccatg gagtggctg accaatact tttgtttat tgcaggatt aagaaaaat ccactggtt tatggcctg actttcacc gagatttgg gagcctgaa aagctcttt cagtacctg gagcgcaaa ctcttcgac gccgcctcc acgctggaa tcggcccaa atggtccgt ttcaacccg actttgggc gaactccgt ccgacaaac tacggccgt atcgccagt ttttactac atctcgcac caaacaatg aaatatttc cacgaccat ttcgaacgt tcaatggtc gaagccgac atcctccac ctcatcagc aacgcatcc gaattccag cacatccaa gtccggaac gacgagctc gacgagctc gatcgcctc cacgaagag ttcagtcaa ttcgaattc aacctagac ccgagcgct gttgtcttc aaagttttg gttctgatc caggctaac atatcacgt gccaaaatc cgcgtctcg tcattggtc tccgactgc gagttcata atgcaaagt gtgacgcga ttggcgcga gcgctattc gaaattgcg gttgataaa aattacgct ttgcaagtg tggcgctgc ttggaggtg gcacgaatg gttgaacag caagcttgg actgacagg catcctttg atgcagttc aaggagttg gagattaag gggcacagg gcacttgat gtgcttcat aatataccg attgaagaa ttacaagag atgacagag cgggaaatt ttggatttg gttagaagt agacatttg gcgagtcgg gttcaccat ttttgcaaa gcttttcca cgtgttaac cttgacgtt tcggttaaa ccgatcaca gagggcgtg atcaggtta cagttgctg attggtgct aacttcagt tgggatagt agtatccat ggtaacgtg cagcattac tacgcgtgg gtggaggac cccacacat gatagtatc tatcatttt gaatcgttt attatcacg aaaaaacaa gtgataagt aaggaacca atcgaattg atttttacg gtgccgttg caaaaaccg cactcaaat gaatatttt gtgacggtt gttaatagc aaatatatg gccgcggaa acgtcctac agaatcgac ttggacaag ttacacctt ttgccaagc tactcaatc cagacgaaa tttttaagc gtgcgacct ttacccaaa acggcctta cataacacc gagtttgaa aatttgtac agttttaca cactttaac gccgtacaa agccaagtg tttcattgc tgtttcaac accgactcc aatgtttta ctcggagca ccgacaggt tccgggaag acaatagtg tcggaaatt tgtattttg cgattattt gcgaaccga cccgagcga aaagttgtc tatattgca ccaatgaag gctctagtt cgcgagcgt gtccttgat tggacgcca aaattcgcc aaaattggt aaaaaagtg gtggaagtg accggtgat gtcacccct cattcttca ctaatctcg acgtctcat attattatt acaacgccg gaaaagtgg gatggaatg agtcgaaat tggcttcag aaggatttt gttaaacaa gtcggactt attataatt gacgaaatt catcttttg gctgaagat cgaggtcca gtgcttgaa gttatcgtt tccaggatg aactacatc aacagtatc aagaatgct aaggtccga atcgtcggc ctttcaacc gccatggcc aacgctggc gatttagcc aactggttg aacatcgaa aaaaaggga ctttacaat tttagttca tccgtacgt cccgtcccc cttgaaata cacctgaag ggtttttcg gccaaaaac tactgtccc cgaatggct acaatgaac cgaccagct tatcaggcc atatgtcaa tacgccccc gaaagtccg accctgata ttcgtctcg tcccgaaaa caaacgcgc atcacctcc tacgaccta atcaagtgt cttttaagc gacactaac ccgaaacaa tggctccac tgccaacag gacgagatc gaacaagtc cgaaaaact ataaccgat tcggaccta agttaccta cttttgttc ggaatcgga atacatcac gcaggcctt caagaccac gaccgcaaa acagtcgaa gaactgttt gtcacgcaa aaaatccag gtgcttgtg gccacggcc accctagca tggggcgtt aatttccca gcccacttg gttatagtt aaagggacc gaatattac gacggtgcg actaaacga tacgttgat atgccggtc accgacgtt ttacaaatg atgggacgg gctggacgt ccacaattc gataccagt ggcgtagcg tgtgttttt gtccaagag agtaaaaaa aatttctat cggaaattt ttattcgaa ccgtttccg gtcgaatca aacttgttg caagtttta cctgaacat gtgaatgcg gaaattgca aatggcacg gtcacgtgt cgctcacaa ttagtcgat ttaatttgt tcgacgttt ttcttccga aggctattg gttaacccg agttattat aaaatggaa ggtggggat gcgaatcaa tttttgaat gaattggcc gattcggtt gccgggcaa ttgcaagaa acgcagtgt atagtgatt cgagatgag gacgtgcaa gacttttac gaaagcact tttttgggc caaatcgca gctcaatac tacctttca tgcaaaact gtgctaaat ctggaccaa aacatgtca cgtgactcc tcattcgac gatctattg gctcacatg tgtcacgtg gaagagtac gccctcttt cccgtacgt cacaacgag gataaaatc aaccgtcat ttgacgctt gatttaaat gtcaaaacg cgccaaccg ttcgattca cctcatttg aaggtttta ctccttatc aagtgttac ctcagtgat aaaaaacta ccaaatcaa gaatatgtc gttgatttg aaaactgtt tttgaccaa gtcattcgg atcatgcag gccatgatc agtctgacg tcacacaaa ggctggctt gattgcacc ctcaaattg atttatttg gggcaaatg ttgatccag ggattgatg atagatgta ggtagtgtg atgatgctg cctcacgtg acgcccgag acacgtgac cggctttcg cgccatctg gcagtggct aacatcaga gtgccccac cttaagtca cttttgcgc caagatcgc aaatcaacg actcggctt ttggatgaa catttcggc gagaaatcg aatgaaatt ttaaagatt ttggcacgt ctaccaaca cttgaaatt aaatttact gtgaaaaat ggcgaaacg aatggcaac attgactat agacttggg gacaacatt gtcactttt gaggctaag gctagaacg acgtgtttg ttcgagttt gaggttttg agggacggg accaacaat ttgagtgtt tatagccga aagtttagt aaacagaag gatgagtct tggtttttt gtcatggct tttgaggac gatttggtg aggttgcag cggtttgcc atcaagaag agtaaaagt ttggaggtt gaagttcaa gtgccgggg gagcgaggg gtttacgag tacgcgggg tactttatg agcgattcc tacatcggg ttggaccag aaggttacg tttaaggtg accgtggtg gattga
- >TC034734 RA
aatgtttcc aataaaaaa catgtaaaa aacaaaaaa aatattgtg gtagataat aaaaacaaa cggtttccc ctgttattg tactgtgat ttatagttt taagcaatg gttattgtt gagggtttt tcacccacc cgtaacttc ttcagcgac ttccacggc ctgcaacgc ctcaatgtg ccccccagt cgccccgat ttgcccgca aagcaaaga ctggcccgc ttgtaccgt tacttcaag tatgaggcc ccgggaccc gggctgagg tgcgtcgtg tacctggag cgcgtcaaa cgtgtcttt agggcacat ttacgcgaa gttgtgagt gaggttatg ttggaggac gtggtcact atgtgtctg ctccaagcg ttgaaagac tacgagagg gacaagagt gaggtcagg ggcgttttg aggaagttg ccccaaaga caattaaag acagtcaag gatgagttg gagtacgtt tgtgataat ctcccgggt gattttttc aaaaagggg tcagagatg gcacggttg ataaaatcg gacgttgaa aaaccggcc gagaagcca cttgatata attttttac attccgatg agtgagaat gagccctgt attgaaggg gaggttgag gagttgagt cttgcggcc gccgcctca acaggggcc ccttactgc gttaaaaaa cgctacgat tattataag agcaaaatg gcggagttt ccgtttgag gaattcaag gaaatcatc ataactgga ataacctca caaaaatca aacgaggaa ctcctcaat tatttcttg gacgttttc gggtgcgaa gttttcgag tttttgaac gaaattatc cagcacaga agtgcacaa attgagtgg ggcgacggg gaaaaaccg gcccccact cagcgaaga ctcgaacca aaagtcaac cgttacacg cctgcctta gccagtcaa gtgatcgtc cagagcgag gaagagaaa aacttagcc aaaacatta cgcaaaatc gaacgcaag caaaaaggg ggtccccaa cccgacttt ttaaacgag cttgagggc gacttaaac tacatcaag caccagccc attttcaga aaggggccc caaactgtg gactatcct catgttcac gaccagttg cgcaatatt tccattacg acgaaattt aacggggtt acgttgaaa cagcccgaa aatacggtt aaaaaggag acttcgacg catgttgag tttacgcta ccggggggc aaaaggcca aaaaatgat gatatagat ttggtgaaa gtcactagt ttggacccg actgggcgt ttggtgttt aaggatata aaggagttt aaccggata cagtcggag gtgtttccg gtggcgtac aatacgaat gaaaatatg ctgatttgt gcccccacg ggggcgggg aagactaat atagcgctg ctggcgatc gtgcaccag attaaggcg catatggaa ggggggctg atccgcaag gatgatttc aagattgtg tatgtgtgc cccatgaaa gcactcgcc accgaaatg gtctcaaac tttagcaaa aaactcgcc cctgtcggt atagtagtg aaagaactg accggcgac atgcaacta accaaaaaa gaaatcgcc gaaacccaa atgttggtc acaacgccc gaaaaatgg gacgtcatc tcgcgcaag ggggctgtg gacaccgaa gtcacaagc ctcgttaaa ttactaatc ctcgacgaa gtccattta ttgaacagt gacaggggc cccgttatt gaagcccta gtcgcgcgc accctgcgc caggtcctc agcagtcaa agcatcatc cgaattgtg gccctttcg gccaccctg cccggctac ctcgacgtt gccaatttc ctcaaagtc aaccctaat acgggcctg tttttcttc gataatcgt tttcgttcc gtccccctg accatgacc ttcattggg gttaaaaat aaaaatgat caagacgcc atggacctc atttgttac aacaaaatc atcccgatt atcaaagat ggccaacaa gttatggtg tttgtgacg tcacgcaac ttgacagct gttgtagcg aagaatttg ctcacacat gcgaaaaat aacaacgtt ttggcgaat tttatcccc gataagaag cacagaatt gggaagaat tttaaaagt agtgagttg gaattgttg gttccgaat ggttttggg gtccaccat gctggcatg tgtcggagt gatcgcttg gaagttgag agtttattc cgtgtgggg gcgcttaaa gtaatagtg tgtactact acccttgcg tggggggtg aatttgccc gcgcatgcg gtcataatc aggggcacg actcgatac gacgcccaa aagtcgtca tacgttgat atggacatg ttggacata cagcagatt ttcgggcgt gccggaagg ccccagtat gacacgtcc ggtcatggt atgattatc acaagtgtg caaaatatg gccaattat atgtcgctt ctgacgtca caagcacca atcgaaagc cagtttttg aacaacgtt cctgatcat ttgaacgcc gagattgtg ctaggaacg gtgtcgaat ttaaaggag gccatggag tggctgacc aatactttt gtttattgc aggattaag aaaaatcca ctggtttat ggcctgact ttcaccgag atttgggag cctgaaaag ctctttcag tacctggag cgcaaactc ttcgacgcc gcctccacg ctggaatcg gcccaaatg gtccgtttc aacccgact ttgggcgaa ctccgtccg acaaactac ggccgtatc gccagtttt tactacatc tcgcaccaa acaatgaaa tatttccac gaccatttc gaacgttca atggtcgaa gccgacatc ctccacctc atcagcaac gcatccgaa ttccagcac atccaagtc cggaacgac gagctcgac gagctcgat cgcctccac gaagagttc agtcaattc gaattcaac ctagacccg agcgctgtt gtcttcaaa gttttggtt ctgatccag gctaacata tcacgtgcc aaaatccgc gtctcgtca ttggtctcc gactgcgag ttcataatg caaagtgtg acgcgattg gcgcgagcg ctattcgaa attgcggtt gataaaaat tacgctttg caagtgtgg cgctgcttg gaggtggca cgaatggtt gaacagcaa gcttggact gacaggcat cctttgatg cagttcaag gagttggag attaagggg cacagggca cttgatgtg cttcataat ataccgatt gaagaatta caagagatg acagagcgg gaaattttg gatttggtt agaagtaga catttggcg agtcgggtt caccatttt tgcaaagct tttccacgt gttaacctt gacgtttcg gttaaaccg atcacagag ggcgtgatc aggttacag ttgctgatt ggtgctaac ttcagttgg gatagtagt atccatggt aacgtgcag cattactac gcgtgggtg gaggacccc acacatgat agtatctat cattttgaa tcgtttatt atcacgaaa aaacaagtg ataagtaag gaaccaatc gaattgatt tttacggtg ccgttgcaa aaaccgcac tcaaatgaa tattttgtg acggttgtt aatagcaaa tatatggcc gcggaaacg tcctacaga atcgacttg gacaagtta caccttttg ccaagctac tcaatccag acgaaattt ttaagcgtg cgaccttta cccaaaacg gccttacat aacaccgag tttgaaaat ttgtacagt tttacacac tttaacgcc gtacaaagc caagtgttt cattgctgt ttcaacacc gactccaat gttttactc ggagcaccg acaggttcc gggaagaca atagtgtcg gaaatttgt attttgcga ttatttgcg aaccgaccc gagcgaaaa gttgtctat attgcacca atgaaggct ctagttcgc gagcgtgtc cttgattgg acgccaaaa ttcgccaaa attggtaaa aaagtggtg gaagtgacc ggtgatgtc acccctcat tcttcacta atctcgacg tctcatatt attattaca acgccggaa aagtgggat ggaatgagt cgaaattgg cttcagaag gattttgtt aaacaagtc ggacttatt ataattgac gaaattcat cttttggct gaagatcga ggtccagtg cttgaagtt atcgtttcc aggatgaac tacatcaac agtatcaag aatgctaag gtccgaatc gtcggcctt tcaaccgcc atggccaac gctggcgat ttagccaac tggttgaac atcgaaaaa aagggactt tacaatttt agttcatcc gtacgtccc gtccccctt gaaatacac ctgaagggt ttttcggcc aaaaactac tgtccccga atggctaca atgaaccga ccagcttat caggccata tgtcaatac gcccccgaa agtccgacc ctgatattc gtctcgtcc cgaaaacaa acgcgcatc acctcctac gacctaatc aagtgtctt ttaagcgac actaacccg aaacaatgg ctccactgc caacaggac gagatcgaa caagtccga aaaactata accgattcg gacctaagt tacctactt ttgttcgga atcggaata catcacgca ggccttcaa gaccacgac cgcaaaaca gtcgaagaa ctgtttgtc acgcaaaaa atccaggtg cttgtggcc acggccacc ctagcatgg ggcgttaat ttcccagcc cacttggtt atagttaaa gggaccgaa tattacgac ggtgcgact aaacgatac gttgatatg ccggtcacc gacgtttta caaatgatg ggacgggct ggacgtcca caattcgat accagtggc gtagcgtgt gtttttgtc caagagagt aaaaaaaat ttctatcgg aaattttta ttcgaaccg tttccggtc gaatcaaac ttgttgcaa gttttacct gaacatgtg aatgcggaa attgcaaat ggcacggtc acgtgtcgc tcacaatta gtcgattta atttgttcg acgtttttc ttccgaagg ctattggtt aacccgagt tattataaa atggaaggt ggggatgcg aatcaattt ttgaatgaa ttggccgat tcggttgcc gggcaattg caagaaacg cagtgtata gtgattcga gatgaggac gtgcaagac ttttacgaa agcactttt ttgggccaa atcgcagct caatactac ctttcatgc aaaactgtg ctaaatctg gaccaaaac atgtcacgt gactcctca ttcgacgat ctattggct cacatgtgt cacgtggaa gagtacgcc ctctttccc gtacgtcac aacgaggat aaaatcaac cgtcatttg acgcttgat ttaaatgtc aaaacgcgc caaccgttc gattcacct catttgaag gttttactc cttatcaag tgttacctc agtgataaa aaactacca aatcaagaa tatgtcgtt gatttgaaa actgttttt gaccaagtc attcggatc atgcaggcc atgatcagt ctgacgtca cacaaaggc tggcttgat tgcaccctc aaattgatt tatttgggg caaatgttg atccaggga ttgatgata gatgtaggt agtgtgatg atgctgcct cacgtgacg cccgagaca cgtgaccgg ctttcgcgc catctggca gtggctaac atcagagtg ccccacctt aagtcactt ttgcgccaa gatcgcaaa tcaacgact cggcttttg gatgaacat ttcggcgag aaatcgaat gaaatttta aagattttg gcacgtcta ccaacactt gaaattaaa tttactgtg aaaaatggc gaaacgaat ggcaacatt gactataga cttggggac aacattgtc acttttgag gctaaggct agaacgacg tgtttgttc gagtttgag gttttgagg gacgggacc aacaatttg agtgtttat agccgaaag tttagtaaa cagaaggat gagtcttgg ttttttgtc atggctttt gaggacgat ttggtgagg ttgcagcgg tttgccatc aagaagagt aaaagtttg gaggttgaa gttcaagtg ccgggggag cgaggggtt tacgagtac gcggggtac tttatgagc gattcctac atcgggttg gaccagaag gttacgttt aaggtgacc gtggtggat tgagtggat gtactagtg gagttatga aattttaaa tgaattgta tttatgttt gtatttttc tgtgtaaat aaaaattta ctttataa
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
