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Gene information
General information
TC034734 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid40: 1558565..1578162
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034734
    iBeetle screen iB_02408
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    Fertility
    fertility after larval injection decreased (no living offspring) -
    iBeetle screen iB_02493
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 4
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    about 50

    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
    appendages sometimes partially remaining - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva dorsal not closed - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva random partiallynot present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_02408
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: % (includes death as pupa, adult)

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034734 RA
      MVIVEGFSP TRNFFSDFH GLQRLNVPP SRPDLPAKQ 
      RLARLYRYF KYEAPGPGL RCVVYLERV KRVFRAHLR 
      EVVSEVMLE DVVTMCLLQ ALKDYERDK SEVRGVLRK 
      LPQRQLKTV KDELEYVCD NLPGDFFKK GSEMARLIK 
      SDVEKPAEK PLDIIFYIP MSENEPCIE GEVEELSLA 
      AAASTGAPY CVKKRYDYY KSKMAEFPF EEFKEIIIT 
      GITSQKSNE ELLNYFLDV FGCEVFEFL NEIIQHRSA 
      QIEWGDGEK PAPTQRRLE PKVNRYTPA LASQVIVQS 
      EEEKNLAKT LRKIERKQK GGPQPDFLN ELEGDLNYI 
      KHQPIFRKG PQTVDYPHV HDQLRNISI TTKFNGVTL 
      KQPENTVKK ETSTHVEFT LPGGKRPKN DDIDLVKVT 
      SLDPTGRLV FKDIKEFNR IQSEVFPVA YNTNENMLI 
      CAPTGAGKT NIALLAIVH QIKAHMEGG LIRKDDFKI 
      VYVCPMKAL ATEMVSNFS KKLAPVGIV VKELTGDMQ 
      LTKKEIAET QMLVTTPEK WDVISRKGA VDTEVTSLV 
      KLLILDEVH LLNSDRGPV IEALVARTL RQVLSSQSI 
      IRIVALSAT LPGYLDVAN FLKVNPNTG LFFFDNRFR 
      SVPLTMTFI GVKNKNDQD AMDLICYNK IIPIIKDGQ 
      QVMVFVTSR NLTAVVAKN LLTHAKNNN VLANFIPDK 
      KHRIGKNFK SSELELLVP NGFGVHHAG MCRSDRLEV 
      ESLFRVGAL KVIVCTTTL AWGVNLPAH AVIIRGTTR 
      YDAQKSSYV DMDMLDIQQ IFGRAGRPQ YDTSGHGMI 
      ITSVQNMAN YMSLLTSQA PIESQFLNN VPDHLNAEI 
      VLGTVSNLK EAMEWLTNT FVYCRIKKN PLVYGLTFT 
      EIWEPEKLF QYLERKLFD AASTLESAQ MVRFNPTLG 
      ELRPTNYGR IASFYYISH QTMKYFHDH FERSMVEAD 
      ILHLISNAS EFQHIQVRN DELDELDRL HEEFSQFEF 
      NLDPSAVVF KVLVLIQAN ISRAKIRVS SLVSDCEFI 
      MQSVTRLAR ALFEIAVDK NYALQVWRC LEVARMVEQ 
      QAWTDRHPL MQFKELEIK GHRALDVLH NIPIEELQE 
      MTEREILDL VRSRHLASR VHHFCKAFP RVNLDVSVK 
      PITEGVIRL QLLIGANFS WDSSIHGNV QHYYAWVED 
      PTHDSIYHF ESFIITKKQ VISKEPIEL IFTVPLQKP 
      HSNEYFVTV VNSKYMAAE TSYRIDLDK LHLLPSYSI 
      QTKFLSVRP LPKTALHNT EFENLYSFT HFNAVQSQV 
      FHCCFNTDS NVLLGAPTG SGKTIVSEI CILRLFANR 
      PERKVVYIA PMKALVRER VLDWTPKFA KIGKKVVEV 
      TGDVTPHSS LISTSHIII TTPEKWDGM SRNWLQKDF 
      VKQVGLIII DEIHLLAED RGPVLEVIV SRMNYINSI 
      KNAKVRIVG LSTAMANAG DLANWLNIE KKGLYNFSS 
      SVRPVPLEI HLKGFSAKN YCPRMATMN RPAYQAICQ 
      YAPESPTLI FVSSRKQTR ITSYDLIKC LLSDTNPKQ 
      WLHCQQDEI EQVRKTITD SDLSYLLLF GIGIHHAGL 
      QDHDRKTVE ELFVTQKIQ VLVATATLA WGVNFPAHL 
      VIVKGTEYY DGATKRYVD MPVTDVLQM MGRAGRPQF 
      DTSGVACVF VQESKKNFY RKFLFEPFP VESNLLQVL 
      PEHVNAEIA NGTVTCRSQ LVDLICSTF FFRRLLVNP 
      SYYKMEGGD ANQFLNELA DSVAGQLQE TQCIVIRDE 
      DVQDFYEST FLGQIAAQY YLSCKTVLN LDQNMSRDS 
      SFDDLLAHM CHVEEYALF PVRHNEDKI NRHLTLDLN 
      VKTRQPFDS PHLKVLLLI KCYLSDKKL PNQEYVVDL 
      KTVFDQVIR IMQAMISLT SHKGWLDCT LKLIYLGQM 
      LIQGLMIDV GSVMMLPHV TPETRDRLS RHLAVANIR 
      VPHLKSLLR QDRKSTTRL LDEHFGEKS NEILKILAR 
      LPTLEIKFT VKNGETNGN IDYRLGDNI VTFEAKART 
      TCLFEFEVL RDGTNNLSV YSRKFSKQK DESWFFVMA 
      FEDDLVRLQ RFAIKKSKS LEVEVQVPG ERGVYEYAG 
      YFMSDSYIG LDQKVTFKV TVVD
    CDS sequence
    • >TC034734 RA
      atggttatt gttgagggt ttttcaccc acccgtaac 
      ttcttcagc gacttccac ggcctgcaa cgcctcaat 
      gtgcccccc agtcgcccc gatttgccc gcaaagcaa 
      agactggcc cgcttgtac cgttacttc aagtatgag 
      gccccggga cccgggctg aggtgcgtc gtgtacctg 
      gagcgcgtc aaacgtgtc tttagggca catttacgc 
      gaagttgtg agtgaggtt atgttggag gacgtggtc 
      actatgtgt ctgctccaa gcgttgaaa gactacgag 
      agggacaag agtgaggtc aggggcgtt ttgaggaag 
      ttgccccaa agacaatta aagacagtc aaggatgag 
      ttggagtac gtttgtgat aatctcccg ggtgatttt 
      ttcaaaaag gggtcagag atggcacgg ttgataaaa 
      tcggacgtt gaaaaaccg gccgagaag ccacttgat 
      ataattttt tacattccg atgagtgag aatgagccc 
      tgtattgaa ggggaggtt gaggagttg agtcttgcg 
      gccgccgcc tcaacaggg gccccttac tgcgttaaa 
      aaacgctac gattattat aagagcaaa atggcggag 
      tttccgttt gaggaattc aaggaaatc atcataact 
      ggaataacc tcacaaaaa tcaaacgag gaactcctc 
      aattatttc ttggacgtt ttcgggtgc gaagttttc 
      gagtttttg aacgaaatt atccagcac agaagtgca 
      caaattgag tggggcgac ggggaaaaa ccggccccc 
      actcagcga agactcgaa ccaaaagtc aaccgttac 
      acgcctgcc ttagccagt caagtgatc gtccagagc 
      gaggaagag aaaaactta gccaaaaca ttacgcaaa 
      atcgaacgc aagcaaaaa gggggtccc caacccgac 
      tttttaaac gagcttgag ggcgactta aactacatc 
      aagcaccag cccattttc agaaagggg ccccaaact 
      gtggactat cctcatgtt cacgaccag ttgcgcaat 
      atttccatt acgacgaaa tttaacggg gttacgttg 
      aaacagccc gaaaatacg gttaaaaag gagacttcg 
      acgcatgtt gagtttacg ctaccgggg ggcaaaagg 
      ccaaaaaat gatgatata gatttggtg aaagtcact 
      agtttggac ccgactggg cgtttggtg tttaaggat 
      ataaaggag tttaaccgg atacagtcg gaggtgttt 
      ccggtggcg tacaatacg aatgaaaat atgctgatt 
      tgtgccccc acgggggcg gggaagact aatatagcg 
      ctgctggcg atcgtgcac cagattaag gcgcatatg 
      gaagggggg ctgatccgc aaggatgat ttcaagatt 
      gtgtatgtg tgccccatg aaagcactc gccaccgaa 
      atggtctca aactttagc aaaaaactc gcccctgtc 
      ggtatagta gtgaaagaa ctgaccggc gacatgcaa 
      ctaaccaaa aaagaaatc gccgaaacc caaatgttg 
      gtcacaacg cccgaaaaa tgggacgtc atctcgcgc 
      aagggggct gtggacacc gaagtcaca agcctcgtt 
      aaattacta atcctcgac gaagtccat ttattgaac 
      agtgacagg ggccccgtt attgaagcc ctagtcgcg 
      cgcaccctg cgccaggtc ctcagcagt caaagcatc 
      atccgaatt gtggccctt tcggccacc ctgcccggc 
      tacctcgac gttgccaat ttcctcaaa gtcaaccct 
      aatacgggc ctgtttttc ttcgataat cgttttcgt 
      tccgtcccc ctgaccatg accttcatt ggggttaaa 
      aataaaaat gatcaagac gccatggac ctcatttgt 
      tacaacaaa atcatcccg attatcaaa gatggccaa 
      caagttatg gtgtttgtg acgtcacgc aacttgaca 
      gctgttgta gcgaagaat ttgctcaca catgcgaaa 
      aataacaac gttttggcg aattttatc cccgataag 
      aagcacaga attgggaag aattttaaa agtagtgag 
      ttggaattg ttggttccg aatggtttt ggggtccac 
      catgctggc atgtgtcgg agtgatcgc ttggaagtt 
      gagagttta ttccgtgtg ggggcgctt aaagtaata 
      gtgtgtact actaccctt gcgtggggg gtgaatttg 
      cccgcgcat gcggtcata atcaggggc acgactcga 
      tacgacgcc caaaagtcg tcatacgtt gatatggac 
      atgttggac atacagcag attttcggg cgtgccgga 
      aggccccag tatgacacg tccggtcat ggtatgatt 
      atcacaagt gtgcaaaat atggccaat tatatgtcg 
      cttctgacg tcacaagca ccaatcgaa agccagttt 
      ttgaacaac gttcctgat catttgaac gccgagatt 
      gtgctagga acggtgtcg aatttaaag gaggccatg 
      gagtggctg accaatact tttgtttat tgcaggatt 
      aagaaaaat ccactggtt tatggcctg actttcacc 
      gagatttgg gagcctgaa aagctcttt cagtacctg 
      gagcgcaaa ctcttcgac gccgcctcc acgctggaa 
      tcggcccaa atggtccgt ttcaacccg actttgggc 
      gaactccgt ccgacaaac tacggccgt atcgccagt 
      ttttactac atctcgcac caaacaatg aaatatttc 
      cacgaccat ttcgaacgt tcaatggtc gaagccgac 
      atcctccac ctcatcagc aacgcatcc gaattccag 
      cacatccaa gtccggaac gacgagctc gacgagctc 
      gatcgcctc cacgaagag ttcagtcaa ttcgaattc 
      aacctagac ccgagcgct gttgtcttc aaagttttg 
      gttctgatc caggctaac atatcacgt gccaaaatc 
      cgcgtctcg tcattggtc tccgactgc gagttcata 
      atgcaaagt gtgacgcga ttggcgcga gcgctattc 
      gaaattgcg gttgataaa aattacgct ttgcaagtg 
      tggcgctgc ttggaggtg gcacgaatg gttgaacag 
      caagcttgg actgacagg catcctttg atgcagttc 
      aaggagttg gagattaag gggcacagg gcacttgat 
      gtgcttcat aatataccg attgaagaa ttacaagag 
      atgacagag cgggaaatt ttggatttg gttagaagt 
      agacatttg gcgagtcgg gttcaccat ttttgcaaa 
      gcttttcca cgtgttaac cttgacgtt tcggttaaa 
      ccgatcaca gagggcgtg atcaggtta cagttgctg 
      attggtgct aacttcagt tgggatagt agtatccat 
      ggtaacgtg cagcattac tacgcgtgg gtggaggac 
      cccacacat gatagtatc tatcatttt gaatcgttt 
      attatcacg aaaaaacaa gtgataagt aaggaacca 
      atcgaattg atttttacg gtgccgttg caaaaaccg 
      cactcaaat gaatatttt gtgacggtt gttaatagc 
      aaatatatg gccgcggaa acgtcctac agaatcgac 
      ttggacaag ttacacctt ttgccaagc tactcaatc 
      cagacgaaa tttttaagc gtgcgacct ttacccaaa 
      acggcctta cataacacc gagtttgaa aatttgtac 
      agttttaca cactttaac gccgtacaa agccaagtg 
      tttcattgc tgtttcaac accgactcc aatgtttta 
      ctcggagca ccgacaggt tccgggaag acaatagtg 
      tcggaaatt tgtattttg cgattattt gcgaaccga 
      cccgagcga aaagttgtc tatattgca ccaatgaag 
      gctctagtt cgcgagcgt gtccttgat tggacgcca 
      aaattcgcc aaaattggt aaaaaagtg gtggaagtg 
      accggtgat gtcacccct cattcttca ctaatctcg 
      acgtctcat attattatt acaacgccg gaaaagtgg 
      gatggaatg agtcgaaat tggcttcag aaggatttt 
      gttaaacaa gtcggactt attataatt gacgaaatt 
      catcttttg gctgaagat cgaggtcca gtgcttgaa 
      gttatcgtt tccaggatg aactacatc aacagtatc 
      aagaatgct aaggtccga atcgtcggc ctttcaacc 
      gccatggcc aacgctggc gatttagcc aactggttg 
      aacatcgaa aaaaaggga ctttacaat tttagttca 
      tccgtacgt cccgtcccc cttgaaata cacctgaag 
      ggtttttcg gccaaaaac tactgtccc cgaatggct 
      acaatgaac cgaccagct tatcaggcc atatgtcaa 
      tacgccccc gaaagtccg accctgata ttcgtctcg 
      tcccgaaaa caaacgcgc atcacctcc tacgaccta 
      atcaagtgt cttttaagc gacactaac ccgaaacaa 
      tggctccac tgccaacag gacgagatc gaacaagtc 
      cgaaaaact ataaccgat tcggaccta agttaccta 
      cttttgttc ggaatcgga atacatcac gcaggcctt 
      caagaccac gaccgcaaa acagtcgaa gaactgttt 
      gtcacgcaa aaaatccag gtgcttgtg gccacggcc 
      accctagca tggggcgtt aatttccca gcccacttg 
      gttatagtt aaagggacc gaatattac gacggtgcg 
      actaaacga tacgttgat atgccggtc accgacgtt 
      ttacaaatg atgggacgg gctggacgt ccacaattc 
      gataccagt ggcgtagcg tgtgttttt gtccaagag 
      agtaaaaaa aatttctat cggaaattt ttattcgaa 
      ccgtttccg gtcgaatca aacttgttg caagtttta 
      cctgaacat gtgaatgcg gaaattgca aatggcacg 
      gtcacgtgt cgctcacaa ttagtcgat ttaatttgt 
      tcgacgttt ttcttccga aggctattg gttaacccg 
      agttattat aaaatggaa ggtggggat gcgaatcaa 
      tttttgaat gaattggcc gattcggtt gccgggcaa 
      ttgcaagaa acgcagtgt atagtgatt cgagatgag 
      gacgtgcaa gacttttac gaaagcact tttttgggc 
      caaatcgca gctcaatac tacctttca tgcaaaact 
      gtgctaaat ctggaccaa aacatgtca cgtgactcc 
      tcattcgac gatctattg gctcacatg tgtcacgtg 
      gaagagtac gccctcttt cccgtacgt cacaacgag 
      gataaaatc aaccgtcat ttgacgctt gatttaaat 
      gtcaaaacg cgccaaccg ttcgattca cctcatttg 
      aaggtttta ctccttatc aagtgttac ctcagtgat 
      aaaaaacta ccaaatcaa gaatatgtc gttgatttg 
      aaaactgtt tttgaccaa gtcattcgg atcatgcag 
      gccatgatc agtctgacg tcacacaaa ggctggctt 
      gattgcacc ctcaaattg atttatttg gggcaaatg 
      ttgatccag ggattgatg atagatgta ggtagtgtg 
      atgatgctg cctcacgtg acgcccgag acacgtgac 
      cggctttcg cgccatctg gcagtggct aacatcaga 
      gtgccccac cttaagtca cttttgcgc caagatcgc 
      aaatcaacg actcggctt ttggatgaa catttcggc 
      gagaaatcg aatgaaatt ttaaagatt ttggcacgt 
      ctaccaaca cttgaaatt aaatttact gtgaaaaat 
      ggcgaaacg aatggcaac attgactat agacttggg 
      gacaacatt gtcactttt gaggctaag gctagaacg 
      acgtgtttg ttcgagttt gaggttttg agggacggg 
      accaacaat ttgagtgtt tatagccga aagtttagt 
      aaacagaag gatgagtct tggtttttt gtcatggct 
      tttgaggac gatttggtg aggttgcag cggtttgcc 
      atcaagaag agtaaaagt ttggaggtt gaagttcaa 
      gtgccgggg gagcgaggg gtttacgag tacgcgggg 
      tactttatg agcgattcc tacatcggg ttggaccag 
      aaggttacg tttaaggtg accgtggtg gattga
    mRNA sequence
    • >TC034734 RA
      aatgtttcc aataaaaaa catgtaaaa aacaaaaaa 
      aatattgtg gtagataat aaaaacaaa cggtttccc 
      ctgttattg tactgtgat ttatagttt taagcaatg 
      gttattgtt gagggtttt tcacccacc cgtaacttc 
      ttcagcgac ttccacggc ctgcaacgc ctcaatgtg 
      ccccccagt cgccccgat ttgcccgca aagcaaaga 
      ctggcccgc ttgtaccgt tacttcaag tatgaggcc 
      ccgggaccc gggctgagg tgcgtcgtg tacctggag 
      cgcgtcaaa cgtgtcttt agggcacat ttacgcgaa 
      gttgtgagt gaggttatg ttggaggac gtggtcact 
      atgtgtctg ctccaagcg ttgaaagac tacgagagg 
      gacaagagt gaggtcagg ggcgttttg aggaagttg 
      ccccaaaga caattaaag acagtcaag gatgagttg 
      gagtacgtt tgtgataat ctcccgggt gattttttc 
      aaaaagggg tcagagatg gcacggttg ataaaatcg 
      gacgttgaa aaaccggcc gagaagcca cttgatata 
      attttttac attccgatg agtgagaat gagccctgt 
      attgaaggg gaggttgag gagttgagt cttgcggcc 
      gccgcctca acaggggcc ccttactgc gttaaaaaa 
      cgctacgat tattataag agcaaaatg gcggagttt 
      ccgtttgag gaattcaag gaaatcatc ataactgga 
      ataacctca caaaaatca aacgaggaa ctcctcaat 
      tatttcttg gacgttttc gggtgcgaa gttttcgag 
      tttttgaac gaaattatc cagcacaga agtgcacaa 
      attgagtgg ggcgacggg gaaaaaccg gcccccact 
      cagcgaaga ctcgaacca aaagtcaac cgttacacg 
      cctgcctta gccagtcaa gtgatcgtc cagagcgag 
      gaagagaaa aacttagcc aaaacatta cgcaaaatc 
      gaacgcaag caaaaaggg ggtccccaa cccgacttt 
      ttaaacgag cttgagggc gacttaaac tacatcaag 
      caccagccc attttcaga aaggggccc caaactgtg 
      gactatcct catgttcac gaccagttg cgcaatatt 
      tccattacg acgaaattt aacggggtt acgttgaaa 
      cagcccgaa aatacggtt aaaaaggag acttcgacg 
      catgttgag tttacgcta ccggggggc aaaaggcca 
      aaaaatgat gatatagat ttggtgaaa gtcactagt 
      ttggacccg actgggcgt ttggtgttt aaggatata 
      aaggagttt aaccggata cagtcggag gtgtttccg 
      gtggcgtac aatacgaat gaaaatatg ctgatttgt 
      gcccccacg ggggcgggg aagactaat atagcgctg 
      ctggcgatc gtgcaccag attaaggcg catatggaa 
      ggggggctg atccgcaag gatgatttc aagattgtg 
      tatgtgtgc cccatgaaa gcactcgcc accgaaatg 
      gtctcaaac tttagcaaa aaactcgcc cctgtcggt 
      atagtagtg aaagaactg accggcgac atgcaacta 
      accaaaaaa gaaatcgcc gaaacccaa atgttggtc 
      acaacgccc gaaaaatgg gacgtcatc tcgcgcaag 
      ggggctgtg gacaccgaa gtcacaagc ctcgttaaa 
      ttactaatc ctcgacgaa gtccattta ttgaacagt 
      gacaggggc cccgttatt gaagcccta gtcgcgcgc 
      accctgcgc caggtcctc agcagtcaa agcatcatc 
      cgaattgtg gccctttcg gccaccctg cccggctac 
      ctcgacgtt gccaatttc ctcaaagtc aaccctaat 
      acgggcctg tttttcttc gataatcgt tttcgttcc 
      gtccccctg accatgacc ttcattggg gttaaaaat 
      aaaaatgat caagacgcc atggacctc atttgttac 
      aacaaaatc atcccgatt atcaaagat ggccaacaa 
      gttatggtg tttgtgacg tcacgcaac ttgacagct 
      gttgtagcg aagaatttg ctcacacat gcgaaaaat 
      aacaacgtt ttggcgaat tttatcccc gataagaag 
      cacagaatt gggaagaat tttaaaagt agtgagttg 
      gaattgttg gttccgaat ggttttggg gtccaccat 
      gctggcatg tgtcggagt gatcgcttg gaagttgag 
      agtttattc cgtgtgggg gcgcttaaa gtaatagtg 
      tgtactact acccttgcg tggggggtg aatttgccc 
      gcgcatgcg gtcataatc aggggcacg actcgatac 
      gacgcccaa aagtcgtca tacgttgat atggacatg 
      ttggacata cagcagatt ttcgggcgt gccggaagg 
      ccccagtat gacacgtcc ggtcatggt atgattatc 
      acaagtgtg caaaatatg gccaattat atgtcgctt 
      ctgacgtca caagcacca atcgaaagc cagtttttg 
      aacaacgtt cctgatcat ttgaacgcc gagattgtg 
      ctaggaacg gtgtcgaat ttaaaggag gccatggag 
      tggctgacc aatactttt gtttattgc aggattaag 
      aaaaatcca ctggtttat ggcctgact ttcaccgag 
      atttgggag cctgaaaag ctctttcag tacctggag 
      cgcaaactc ttcgacgcc gcctccacg ctggaatcg 
      gcccaaatg gtccgtttc aacccgact ttgggcgaa 
      ctccgtccg acaaactac ggccgtatc gccagtttt 
      tactacatc tcgcaccaa acaatgaaa tatttccac 
      gaccatttc gaacgttca atggtcgaa gccgacatc 
      ctccacctc atcagcaac gcatccgaa ttccagcac 
      atccaagtc cggaacgac gagctcgac gagctcgat 
      cgcctccac gaagagttc agtcaattc gaattcaac 
      ctagacccg agcgctgtt gtcttcaaa gttttggtt 
      ctgatccag gctaacata tcacgtgcc aaaatccgc 
      gtctcgtca ttggtctcc gactgcgag ttcataatg 
      caaagtgtg acgcgattg gcgcgagcg ctattcgaa 
      attgcggtt gataaaaat tacgctttg caagtgtgg 
      cgctgcttg gaggtggca cgaatggtt gaacagcaa 
      gcttggact gacaggcat cctttgatg cagttcaag 
      gagttggag attaagggg cacagggca cttgatgtg 
      cttcataat ataccgatt gaagaatta caagagatg 
      acagagcgg gaaattttg gatttggtt agaagtaga 
      catttggcg agtcgggtt caccatttt tgcaaagct 
      tttccacgt gttaacctt gacgtttcg gttaaaccg 
      atcacagag ggcgtgatc aggttacag ttgctgatt 
      ggtgctaac ttcagttgg gatagtagt atccatggt 
      aacgtgcag cattactac gcgtgggtg gaggacccc 
      acacatgat agtatctat cattttgaa tcgtttatt 
      atcacgaaa aaacaagtg ataagtaag gaaccaatc 
      gaattgatt tttacggtg ccgttgcaa aaaccgcac 
      tcaaatgaa tattttgtg acggttgtt aatagcaaa 
      tatatggcc gcggaaacg tcctacaga atcgacttg 
      gacaagtta caccttttg ccaagctac tcaatccag 
      acgaaattt ttaagcgtg cgaccttta cccaaaacg 
      gccttacat aacaccgag tttgaaaat ttgtacagt 
      tttacacac tttaacgcc gtacaaagc caagtgttt 
      cattgctgt ttcaacacc gactccaat gttttactc 
      ggagcaccg acaggttcc gggaagaca atagtgtcg 
      gaaatttgt attttgcga ttatttgcg aaccgaccc 
      gagcgaaaa gttgtctat attgcacca atgaaggct 
      ctagttcgc gagcgtgtc cttgattgg acgccaaaa 
      ttcgccaaa attggtaaa aaagtggtg gaagtgacc 
      ggtgatgtc acccctcat tcttcacta atctcgacg 
      tctcatatt attattaca acgccggaa aagtgggat 
      ggaatgagt cgaaattgg cttcagaag gattttgtt 
      aaacaagtc ggacttatt ataattgac gaaattcat 
      cttttggct gaagatcga ggtccagtg cttgaagtt 
      atcgtttcc aggatgaac tacatcaac agtatcaag 
      aatgctaag gtccgaatc gtcggcctt tcaaccgcc 
      atggccaac gctggcgat ttagccaac tggttgaac 
      atcgaaaaa aagggactt tacaatttt agttcatcc 
      gtacgtccc gtccccctt gaaatacac ctgaagggt 
      ttttcggcc aaaaactac tgtccccga atggctaca 
      atgaaccga ccagcttat caggccata tgtcaatac 
      gcccccgaa agtccgacc ctgatattc gtctcgtcc 
      cgaaaacaa acgcgcatc acctcctac gacctaatc 
      aagtgtctt ttaagcgac actaacccg aaacaatgg 
      ctccactgc caacaggac gagatcgaa caagtccga 
      aaaactata accgattcg gacctaagt tacctactt 
      ttgttcgga atcggaata catcacgca ggccttcaa 
      gaccacgac cgcaaaaca gtcgaagaa ctgtttgtc 
      acgcaaaaa atccaggtg cttgtggcc acggccacc 
      ctagcatgg ggcgttaat ttcccagcc cacttggtt 
      atagttaaa gggaccgaa tattacgac ggtgcgact 
      aaacgatac gttgatatg ccggtcacc gacgtttta 
      caaatgatg ggacgggct ggacgtcca caattcgat 
      accagtggc gtagcgtgt gtttttgtc caagagagt 
      aaaaaaaat ttctatcgg aaattttta ttcgaaccg 
      tttccggtc gaatcaaac ttgttgcaa gttttacct 
      gaacatgtg aatgcggaa attgcaaat ggcacggtc 
      acgtgtcgc tcacaatta gtcgattta atttgttcg 
      acgtttttc ttccgaagg ctattggtt aacccgagt 
      tattataaa atggaaggt ggggatgcg aatcaattt 
      ttgaatgaa ttggccgat tcggttgcc gggcaattg 
      caagaaacg cagtgtata gtgattcga gatgaggac 
      gtgcaagac ttttacgaa agcactttt ttgggccaa 
      atcgcagct caatactac ctttcatgc aaaactgtg 
      ctaaatctg gaccaaaac atgtcacgt gactcctca 
      ttcgacgat ctattggct cacatgtgt cacgtggaa 
      gagtacgcc ctctttccc gtacgtcac aacgaggat 
      aaaatcaac cgtcatttg acgcttgat ttaaatgtc 
      aaaacgcgc caaccgttc gattcacct catttgaag 
      gttttactc cttatcaag tgttacctc agtgataaa 
      aaactacca aatcaagaa tatgtcgtt gatttgaaa 
      actgttttt gaccaagtc attcggatc atgcaggcc 
      atgatcagt ctgacgtca cacaaaggc tggcttgat 
      tgcaccctc aaattgatt tatttgggg caaatgttg 
      atccaggga ttgatgata gatgtaggt agtgtgatg 
      atgctgcct cacgtgacg cccgagaca cgtgaccgg 
      ctttcgcgc catctggca gtggctaac atcagagtg 
      ccccacctt aagtcactt ttgcgccaa gatcgcaaa 
      tcaacgact cggcttttg gatgaacat ttcggcgag 
      aaatcgaat gaaatttta aagattttg gcacgtcta 
      ccaacactt gaaattaaa tttactgtg aaaaatggc 
      gaaacgaat ggcaacatt gactataga cttggggac 
      aacattgtc acttttgag gctaaggct agaacgacg 
      tgtttgttc gagtttgag gttttgagg gacgggacc 
      aacaatttg agtgtttat agccgaaag tttagtaaa 
      cagaaggat gagtcttgg ttttttgtc atggctttt 
      gaggacgat ttggtgagg ttgcagcgg tttgccatc 
      aagaagagt aaaagtttg gaggttgaa gttcaagtg 
      ccgggggag cgaggggtt tacgagtac gcggggtac 
      tttatgagc gattcctac atcgggttg gaccagaag 
      gttacgttt aaggtgacc gtggtggat tgagtggat 
      gtactagtg gagttatga aattttaaa tgaattgta 
      tttatgttt gtatttttc tgtgtaaat aaaaattta 
      ctttataa