Status
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Gene information
General information
TC015567 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 2461477..2463973
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015567
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02492

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 11.11% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 22.22% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015567 RA
      MRTVPNDLF VYLGLFAVL FSGLIAFYT IVPQNYGPR 
      PQKPHIIII MADDVGRND VGFYGSGQI PTPSIDALA 
      YNGIILDRF YTQCSCTPS RAALLTGQY PIRLGMQGL 
      PIRAGENKS LPLDVVTMP QYLKRLGYK THLVGKWHL 
      GYAHIEDTP LQRGFDSHF GYWNGFVGY FNYTAVYEL 
      ANDTMVKGF DLFDGVVPA WQERGKYAT DLFTHKAMK 
      IIDEHNSEK PLFLVLAHL AGHTGEDGV ELGVPDVAQ 
      AETRFSFIK NPKRRLYAD IVSRLDDSV GQIVEKLDE 
      KNLLDNSII LFLSDNGAQ TIGVYENSG SNWPLRGLK 
      LTEFEGGIR APAAIYSPL FHQKGYVSK ELIHITDFL 
      PTFYAAAGG NLEYLGKID GVNQWEVLS QQKPGKRDE 
      ILVNFNEID NVSAIIMGQ YKLVQGRLA FPEYGDYHG 
      DSGRNPEDP PYNFTAVLS KNINQQNRN KIESLRNQT 
      DLSWCRGEK NQPLIDCTE GCLFNLESD PCETTNIIQ 
      EEKIIAGKL LGRLFEFSQ ILVPQQVAI FDPESNPEF 
      YNGTWCTWL DPQFCPKTI
    CDS sequence
    • >TC015567 RA
      atgaggaca gtaccgaac gatttgttc gtctattta 
      ggccttttt gctgtgtta ttttccggc ctgatcgcc 
      ttttacaca atagtaccg caaaactat gggccacgg 
      ccacaaaaa ccccacatt atcataata atggccgac 
      gatgtgggt cgtaacgat gtcggtttt tacggttcg 
      ggtcaaatc ccgacccca agtatcgac gctttggct 
      tacaatggc attattctg gaccggttt tacactcaa 
      tgttcgtgt accccatcc cgagcggcc cttctaact 
      gggcagtac cccattaga ttgggtatg caagggttg 
      ccgataagg gccggcgag aataaatcg ttgcctctt 
      gatgttgtc acaatgccc caatatttg aaacgtttg 
      gggtacaag acccatttg gtcgggaaa tggcatttg 
      gggtatgcc catatagag gataccccg ttgcaaagg 
      gggtttgat agtcatttt gggtactgg aacgggttt 
      gtgggttat ttcaactat acggcggtt tatgaactg 
      gcaaatgat acgatggtt aaagggttc gatttgttt 
      gatggggtc gtaccagcg tggcaagaa cgtggcaag 
      tacgccact gatttattt acacacaag gctatgaaa 
      atcatagat gaacacaat agtgagaaa ccgttgttt 
      ttggttttg gcccatttg gctgggcat acgggcgaa 
      gatggggtc gagttgggt gtgcctgat gttgcacag 
      gccgaaacg aggttctcg tttattaaa aatcccaaa 
      cggcgactt tacgccgac attgtgtca cgtctggac 
      gattctgtt ggtcagatt gtggaaaaa ttggacgaa 
      aaaaacctt ctagacaac tcgatcatt cttttctta 
      tcagataac ggggcacaa actatcggg gtgtacgaa 
      aattccggc tcgaattgg cctttaaga ggactcaaa 
      ttgaccgaa tttgagggc ggaattcgg gccccagcg 
      gcgatttac agcccactt ttccaccaa aaaggctac 
      gtgagtaaa gaactgata cacatcact gattttttg 
      cccactttt tacgccgcc gctggtggc aatttggaa 
      tatttgggc aaaatcgat ggtgtgaac cagtgggag 
      gttttatcg cagcaaaag ccgggcaaa agggacgaa 
      attctggtc aattttaac gaaattgac aacgtttcg 
      gccataatt atgggacag tacaagctg gttcaaggg 
      agacttgct tttcccgaa tatggggac taccatggt 
      gacagtgga cgtaatcct gaggaccca ccgtataat 
      ttcactgcg gttttgtcc aagaatatt aaccagcaa 
      aatagaaac aaaattgaa agtttgagg aatcaaact 
      gacttgagc tggtgcagg ggtgaaaaa aaccaaccc 
      ctaatcgat tgtacagag ggttgtttg tttaatcta 
      gagagtgac ccttgtgag accactaat atcattcag 
      gaggaaaaa ataatagcg gggaaatta cttggacga 
      ctttttgaa ttttcccaa attttggtc ccccagcaa 
      gtggcgatt tttgatcca gaatcgaat cccgaattt 
      tacaacggg acttggtgc acgtggctc gatccacag 
      ttttgtccc aaaactatt taa
    mRNA sequence
    • >TC015567 RA
      tctcagtta ctaaaatga ggacagtac cgaacgatt 
      tgttcgtct atttaggcc tttttgctg tgttatttt 
      ccggcctga tcgcctttt acacaatag taccgcaaa 
      actatgggc cacggccac aaaaacccc acattatca 
      taataatgg ccgacgatg tgggtcgta acgatgtcg 
      gtttttacg gttcgggtc aaatcccga ccccaagta 
      tcgacgctt tggcttaca atggcatta ttctggacc 
      ggttttaca ctcaatgtt cgtgtaccc catcccgag 
      cggcccttc taactgggc agtacccca ttagattgg 
      gtatgcaag ggttgccga taagggccg gcgagaata 
      aatcgttgc ctcttgatg ttgtcacaa tgccccaat 
      atttgaaac gtttggggt acaagaccc atttggtcg 
      ggaaatggc atttggggt atgcccata tagaggata 
      ccccgttgc aaagggggt ttgatagtc attttgggt 
      actggaacg ggtttgtgg gttatttca actatacgg 
      cggtttatg aactggcaa atgatacga tggttaaag 
      ggttcgatt tgtttgatg gggtcgtac cagcgtggc 
      aagaacgtg gcaagtacg ccactgatt tatttacac 
      acaaggcta tgaaaatca tagatgaac acaatagtg 
      agaaaccgt tgtttttgg ttttggccc atttggctg 
      ggcatacgg gcgaagatg gggtcgagt tgggtgtgc 
      ctgatgttg cacaggccg aaacgaggt tctcgttta 
      ttaaaaatc ccaaacggc gactttacg ccgacattg 
      tgtcacgtc tggacgatt ctgttggtc agattgtgg 
      aaaaattgg acgaaaaaa accttctag acaactcga 
      tcattcttt tcttatcag ataacgggg cacaaacta 
      tcggggtgt acgaaaatt ccggctcga attggcctt 
      taagaggac tcaaattga ccgaatttg agggcggaa 
      ttcgggccc cagcggcga tttacagcc cacttttcc 
      accaaaaag gctacgtga gtaaagaac tgatacaca 
      tcactgatt ttttgccca ctttttacg ccgccgctg 
      gtggcaatt tggaatatt tgggcaaaa tcgatggtg 
      tgaaccagt gggaggttt tatcgcagc aaaagccgg 
      gcaaaaggg acgaaattc tggtcaatt ttaacgaaa 
      ttgacaacg tttcggcca taattatgg gacagtaca 
      agctggttc aagggagac ttgcttttc ccgaatatg 
      gggactacc atggtgaca gtggacgta atcctgagg 
      acccaccgt ataatttca ctgcggttt tgtccaaga 
      atattaacc agcaaaata gaaacaaaa ttgaaagtt 
      tgaggaatc aaactgact tgagctggt gcaggggtg 
      aaaaaaacc aacccctaa tcgattgta cagagggtt 
      gtttgttta atctagaga gtgaccctt gtgagacca 
      ctaatatca ttcaggagg aaaaaataa tagcgggga 
      aattacttg gacgacttt ttgaatttt cccaaattt 
      tggtccccc agcaagtgg cgatttttg atccagaat 
      cgaatcccg aattttaca acgggactt ggtgcacgt 
      ggctcgatc cacagtttt gtcccaaaa ctatttaac 
      tttgtgtaa ttggttatc atttgtaca taaatgaaa 
      ataaacact gggtaattg g
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY