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Gene information
General information
TC015557 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 2297256..2299193
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015557
    iBeetle screen iB_02490

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015557 RA
      MKCGGRFIT KLLIQLMAV GIIIFTIIQ FRPIKLDMT 
      VPHLNSRFL LQKRLDPLT RHADSTTES NDLEPFVDK 
      SPSLSEDKL VEDIQKRMP NLPIVYWNK NKNKPMGMN 
      HSCARFPSM FDLEFNNLY WQTLRTSNG TFQLFGAYL 
      DKRANNRLG PTVRILGMI DRIEPTVVT YCQFWFDGK 
      KEPVVAKSF EYKYIWYKK WGNYKQGIY QPYLTACVI 
      PQHYRDKIP QSISLVEKA CDNSTNNLR VIFEKPTQK 
      KDFAVCVKG LDFLHEDLS VRLIEWIEL LNLLGADKV 
      FFYELQVHP NISKVLRHY EQEGKVHVT PINLAGGQP 
      NAPSFQHLY LTKKTNHKR QNELIPYND CFYKHMYEY 
      KYIALLDID EVIMPLEGT TWKGLMDKV LPKALKINK 
      EERASYNVR NVYFLDDLL HNHGWFKEI PKYMHMLQH 
      VYRAKNFTK PGQYVKCFH NTEKVLTLH NHFPLSCLG 
      SGCTSYPIE TSDAQLHHY RADCVKTLK KSCEEYRKT 
      SVMDTTIWK FKQPLVGRV TATLRTLGY FANQEKDSK 
      R
    CDS sequence
    • >TC015557 RA
      atgaagtgc ggaggccgc ttcatcacg aaactcctc 
      atccagctg atggccgtc ggcataatc atcttcacc 
      atcatccaa ttccgcccc atcaagctc gacatgacc 
      gtcccccac ctcaacagc cgcttcctc ctccagaag 
      cggctcgac cccctcacc cgccacgcc gactccacg 
      accgaaagc aacgacctt gagccgttc gtcgacaag 
      agcccatcc ctgtccgag gacaagctg gtcgaggac 
      atccagaag cgcatgccc aacctcccc atcgtctac 
      tggaacaag aacaagaac aagccgatg ggcatgaac 
      cactcctgc gcccgcttc cccagcatg ttcgacctg 
      gaattcaac aacctctac tggcagacg ctgcgcacc 
      tccaacggc acgttccag ctcttcggc gcctatttg 
      gacaaaagg gccaacaat aggctgggc cccactgtg 
      cgcatcctg ggcatgatc gacaggatc gaacccacg 
      gtggtgacc tattgccag ttttggttc gacgggaaa 
      aaggaacca gtggtggcg aagtcgttc gagtacaag 
      tacatttgg tacaagaag tgggggaac tacaagcag 
      gggatttac cagccgtat ttgacagcg tgtgtcatc 
      ccccaacac tatagggac aaaatcccc caaagtata 
      tcactagtg gagaaggct tgtgacaac tcaactaat 
      aatctgaga gtgattttt gaaaaacca acacagaaa 
      aaagacttt gctgtgtgt gtcaaaggt ttggacttc 
      ctccatgag gacttgtcc gtgcgtttg atcgaatgg 
      atcgaactt ttgaacctt ctaggtgcc gataaagtg 
      ttcttttac gaactccag gtgcaccct aacattagc 
      aaagtgctg cgacattac gagcaagag ggcaaagtg 
      cacgtcacc cccattaac ctagccggg gggcaaccc 
      aacgccccc tccttccag cacttgtac ctcaccaag 
      aagaccaac cacaagcgc cagaacgag ctgatccct 
      tacaacgac tgcttctac aagcacatg tacgagtac 
      aagtacatc gcccttttg gacatcgac gaggtgatc 
      atgcctctg gagggcacc acctggaag ggcctcatg 
      gacaaggtc ctgcccaag gccctcaag atcaacaag 
      gaggaaagg gcgtcttac aacgtccgc aacgtctac 
      ttcctggac gatctactc cacaaccac ggctggttc 
      aaggagatc cccaagtac atgcacatg ctgcagcac 
      gtctaccgg gccaagaac ttcaccaag cccggacag 
      tacgtcaag tgtttccac aatacggaa aaagtgcta 
      actttgcac aaccatttc ccgctctcg tgtctggga 
      agtgggtgt acgtcgtat cccattgag acaagtgac 
      gctcaactg caccattac cgcgccgac tgcgtcaaa 
      acgctgaag aagagctgc gaggagtac aggaagacg 
      agtgtgatg gacaccacc atttggaag tttaagcag 
      ccgttggtg gggcgtgtg acggccact ttgcggact 
      ttgggatat tttgccaat caggagaag gacagtaag 
      cggtga
    mRNA sequence
    • >TC015557 RA
      ttgctcaac cttgaccaa aattcacgt agaattctg 
      cgttctaat ccctttttt ttcatttct ccttgttcc 
      agccgccag catgaagtg cggaggccg cttcatcac 
      gaaactcct catccagct gatggccgt cggcataat 
      catcttcac catcatcca attccgccc catcaagct 
      cgacatgac cgtccccca cctcaacag ccgcttcct 
      cctccagaa gcggctcga ccccctcac ccgccacgc 
      cgactccac gaccgaaag caacgacct tgagccgtt 
      cgtcgacaa gagcccatc cctgtccga ggacaagct 
      ggtcgagga catccagaa gcgcatgcc caacctccc 
      catcgtcta ctggaacaa gaacaagaa caagccgat 
      gggcatgaa ccactcctg cgcccgctt ccccagcat 
      gttcgacct ggaattcaa caacctcta ctggcagac 
      gctgcgcac ctccaacgg cacgttcca gctcttcgg 
      cgcctattt ggacaaaag ggccaacaa taggctggg 
      ccccactgt gcgcatcct gggcatgat cgacaggat 
      cgaacccac ggtggtgac ctattgcca gttttggtt 
      cgacgggaa aaaggaacc agtggtggc gaagtcgtt 
      cgagtacaa gtacatttg gtacaagaa gtgggggaa 
      ctacaagca ggggattta ccagccgta tttgacagc 
      gtgtgtcat cccccaaca ctataggga caaaatccc 
      ccaaagtat atcactagt ggagaaggc ttgtgacaa 
      ctcaactaa taatctgag agtgatttt tgaaaaacc 
      aacacagaa aaaagactt tgctgtgtg tgtcaaagg 
      tttggactt cctccatga ggacttgtc cgtgcgttt 
      gatcgaatg gatcgaact tttgaacct tctaggtgc 
      cgataaagt gttctttta cgaactcca ggtgcaccc 
      taacattag caaagtgct gcgacatta cgagcaaga 
      gggcaaagt gcacgtcac ccccattaa cctagccgg 
      ggggcaacc caacgcccc ctccttcca gcacttgta 
      cctcaccaa gaagaccaa ccacaagcg ccagaacga 
      gctgatccc ttacaacga ctgcttcta caagcacat 
      gtacgagta caagtacat cgccctttt ggacatcga 
      cgaggtgat catgcctct ggagggcac cacctggaa 
      gggcctcat ggacaaggt cctgcccaa ggccctcaa 
      gatcaacaa ggaggaaag ggcgtctta caacgtccg 
      caacgtcta cttcctgga cgatctact ccacaacca 
      cggctggtt caaggagat ccccaagta catgcacat 
      gctgcagca cgtctaccg ggccaagaa cttcaccaa 
      gcccggaca gtacgtcaa gtgtttcca caatacgga 
      aaaagtgct aactttgca caaccattt cccgctctc 
      gtgtctggg aagtgggtg tacgtcgta tcccattga 
      gacaagtga cgctcaact gcaccatta ccgcgccga 
      ctgcgtcaa aacgctgaa gaagagctg cgaggagta 
      caggaagac gagtgtgat ggacaccac catttggaa 
      gtttaagca gccgttggt ggggcgtgt gacggccac 
      tttgcggac tttgggata ttttgccaa tcaggagaa 
      ggacagtaa gcggtgatg attgcgtgc tcaatttac 
      aagttccag aaaattgtg ataaattgt tggatgcca 
      tggaggttt tttgctcaa tttggttgc gttttgcgt 
      aggttaagg catcgctcg agtgtatat agatgaggc 
      gagtttgca ttgtgcctt taggtaata gatgtaagg 
      attaactat ttattctat gtaagttat ttataaacg 
      cattaagag gaataaaac gattttcga att
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY