Status
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Gene information
General information
TC015533 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 2020824..2022731
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015533
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02487

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015533 RA
      MANLYATQP LGRELSIYL ARHLLSGHS IGNPVIVNI 
      LNNTIIHVI PVIDTAFEQ IWGGYEKQA PGNARPDHY 
      TCNNITADF KQVGDQVIG GARVNGELK SVANAFKHL 
      LMEEKFDLV LNIEGGSGR VIYPKTGTK VNIFENYAE 
      KYLKSFKNR PECHRNVKG TDQTITDGI YSEFGVPVI 
      TGKVTCCEY PSVVDIPYI WREALAPLM TVLTFATTG 
      IQGFVIDER KQPMRNATL RIVGYNRFD ITRQNAKFK 
      IMLPEGRYI VEVSCHGYA NATFDTAVE NGKFVYLPV 
      ILQRNLNET TPGIRGYVR DSYNHPIAA AVITIVEKN 
      LTIESDSEG KYSVAMAPG TYTLNVSAP GFHPSVKYF 
      TIDGVVGSH VVMFTLVKD KTVWGLPRL AFVIMMGFV 
      CFGTLGLLL MFYVACKRK SEYGPVSQN PFYEDFKDF 
      DESKETEVF ARPLPVKPI TRPYFDDDD DTDADASSE 
      DEENEFDLL HSK
    CDS sequence
    • >TC015533 RA
      atggccaac ttgtacgcc actcaacca ttgggtcgc 
      gaattgtcg atttatctg gcacgccat cttttgagc 
      ggccactca attggcaat cctgtcatt gtcaatatt 
      ttaaataac acaattata cacgtaata cctgtcatt 
      gatacggca ttcgaacaa atctggggt ggctatgaa 
      aaacaagca cctggcaac gcacgacct gatcactac 
      acctgtaat aatatcaca gccgacttt aaacaagtc 
      ggtgatcag gtcataggg ggcgcccgt gtcaacggt 
      gaactcaaa tcggtggct aacgccttc aaacatctc 
      ctaatggag gaaaaattc gatttggtc ttgaacatt 
      gaaggcggc tctggacga gtaatttac ccgaaaact 
      gggactaaa gttaatatt ttcgaaaac tacgcggaa 
      aaatattta aaatcgttc aaaaaccgg ccagagtgt 
      caccgaaac gtcaaagga accgatcag acaatcact 
      gacggtatt tatagcgag tttggcgtt ccggttata 
      accggtaaa gtgacatgt tgcgagtat ccttcagtt 
      gttgacatt ccctacatt tggcgtgag gctttagcg 
      ccgctaatg acagttttg acgtttgcg acgacaggc 
      atccagggt tttgtgatc gacgagcgc aaacaaccg 
      atgcgtaac gcaacctta cgtattgtc ggttataac 
      cggtttgat atcacgcgc caaaacgcc aaatttaaa 
      atcatgtta ccggaggga cgctatatt gttgaagtg 
      tcgtgtcat ggttacgcc aacgccacc tttgacacg 
      gccgtggaa aatggcaaa tttgtttat ttgccggtg 
      attttgcaa cggaattta aatgaaaca acgcctggt 
      atcaggggt tatgtcaga gatagttac aatcatccg 
      atcgcagcg gctgtgatt acaattgtc gagaaaaat 
      ttaacaatt gaatcggac agtgaaggg aagtacagt 
      gttgccatg gctcccggg acgtacact ttgaacgtg 
      tcagcgcct ggctttcac ccgagtgtt aaatatttc 
      acgatagat ggcgttgtg ggttcacat gtggtgatg 
      tttactcta gtcaaggat aaaacagtg tggggtttg 
      ccccgattg gcgtttgtt attatgatg ggattcgtt 
      tgtttcggc actttgggc ctacttttg atgttttac 
      gttgcctgt aaaaggaag agtgaatat ggaccggtg 
      tcccaaaat cccttctat gaagatttc aaggatttt 
      gacgaatca aaggagacg gaagtgttt gcaagaccg 
      ttgccagtt aagccaatc acaaggccc tattttgat 
      gatgatgat gatacagac gctgatgcg agttctgag 
      gatgaggag aatgagttt gatttgttg cattccaag 
      tag
    mRNA sequence
    • >TC015533 RA
      taacaaaat taccctaat taaaaatcg attaataac 
      acatttaat tgctccaat ttcagattg attcatccg 
      acgaactca aattccaca ttgcgatca tggccaact 
      tgtacgcca ctcaaccat tgggtcgcg aattgtcga 
      tttatctgg cacgccatc ttttgagcg gccactcaa 
      ttggcaatc ctgtcattg tcaatattt taaataaca 
      caattatac acgtaatac ctgtcattg atacggcat 
      tcgaacaaa tctggggtg gctatgaaa aacaagcac 
      ctggcaacg cacgacctg atcactaca cctgtaata 
      atatcacag ccgacttta aacaagtcg gtgatcagg 
      tcatagggg gcgcccgtg tcaacggtg aactcaaat 
      cggtggcta acgccttca aacatctcc taatggagg 
      aaaaattcg atttggtct tgaacattg aaggcggct 
      ctggacgag taatttacc cgaaaactg ggactaaag 
      ttaatattt tcgaaaact acgcggaaa aatatttaa 
      aatcgttca aaaaccggc cagagtgtc accgaaacg 
      tcaaaggaa ccgatcaga caatcactg acggtattt 
      atagcgagt ttggcgttc cggttataa ccggtaaag 
      tgacatgtt gcgagtatc cttcagttg ttgacattc 
      cctacattt ggcgtgagg ctttagcgc cgctaatga 
      cagttttga cgtttgcga cgacaggca tccagggtt 
      ttgtgatcg acgagcgca aacaaccga tgcgtaacg 
      caaccttac gtattgtcg gttataacc ggtttgata 
      tcacgcgcc aaaacgcca aatttaaaa tcatgttac 
      cggagggac gctatattg ttgaagtgt cgtgtcatg 
      gttacgcca acgccacct ttgacacgg ccgtggaaa 
      atggcaaat ttgtttatt tgccggtga ttttgcaac 
      ggaatttaa atgaaacaa cgcctggta tcaggggtt 
      atgtcagag atagttaca atcatccga tcgcagcgg 
      ctgtgatta caattgtcg agaaaaatt taacaattg 
      aatcggaca gtgaaggga agtacagtg ttgccatgg 
      ctcccggga cgtacactt tgaacgtgt cagcgcctg 
      gctttcacc cgagtgtta aatatttca cgatagatg 
      gcgttgtgg gttcacatg tggtgatgt ttactctag 
      tcaaggata aaacagtgt ggggtttgc cccgattgg 
      cgtttgtta ttatgatgg gattcgttt gtttcggca 
      ctttgggcc tacttttga tgttttacg ttgcctgta 
      aaaggaaga gtgaatatg gaccggtgt cccaaaatc 
      ccttctatg aagatttca aggattttg acgaatcaa 
      aggagacgg aagtgtttg caagaccgt tgccagtta 
      agccaatca caaggccct attttgatg atgatgatg 
      atacagacg ctgatgcga gttctgagg atgaggaga 
      atgagtttg atttgttgc attccaagt agttgatat 
      tttttgagc gttttttac gatctctag tgatgaaat 
      ctctaaaga attattttt ttaacactt ttttgtaca 
      tagttaaga gttaaaaca tcaccaagt gaggtggtt 
      agtgcttcc atttacata agagaccac tgttgcagt 
      attttatca ataacaagt tactaatag ttttcattt 
      tttctttta ttaaaacct ccaagttga a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY