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Gene information
General information
TC015513 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid40: 693045..694702
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015513
    iBeetle screen iB_02485
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015513 RA
      MASNRSLHL DLELRTRDE SQIPLNHTP TSPTDQYYR 
      SIFDRADTN KDGVVTPNE LERVVDRLS LTDEPPPQQ 
      LIRQIHQMA DKNQDGGLD YGEFVTMIK TQPLFKKVV 
      TRYVNFILP RKKQGAVAT QVDGAGEYE DQYKFCPPK 
      IGMITISII ELIFFAIDE YRERNSTLF AAGPMAQLF 
      IYDPERRYE AWRYLTYMF VHIGYLHLV VNLVVQVFL 
      GIPLEMVHD WWRVLLVYV TGVVAGSLG TSITDPRAK 
      LAGASGGVY SLITAHIAT IIMNWHEMS MPFVQLAIF 
      LLITGVDVG TSIYNRYWA NLDEPIGYM AHFSGALAG 
      LLVGICVLK NLQITKKEN IIWWVSIAV YLALMVGAV 
      VWNIFYSDY FPTQK
    CDS sequence
    • >TC015513 RA
      atggcttcg aacaggtcg cttcacctg gacctggaa 
      ttaagaact agggatgaa tcccaaatt cccctcaac 
      cacacccct acgtcaccc acggaccaa tactatcgc 
      agtattttt gacagggct gacactaac aaggacggg 
      gtagtgacc cccaacgag ctcgagcgg gtggtggac 
      cgcttgagc ctcaccgat gagccccca ccgcaacaa 
      ctaatccgc caaatccac caaatggcc gacaagaac 
      caagatggt ggactagat tatggggaa tttgtcaca 
      atgataaaa acacagccc ctctttaag aaagttgta 
      acccgttat gtgaatttc atcctcccg aggaaaaaa 
      cagggggct gtggccacc caagtcgac ggtgccggc 
      gaatacgaa gaccagtac aaattttgc ccgccaaaa 
      atcggaatg atcacaatt tcaatcatc gagttgatt 
      tttttcgcc attgatgaa taccgtgaa cgcaactcc 
      accctattt gccgctgga ccaatggcc caattgttt 
      atttacgac ccggaacgg cgttacgag gcttggcgc 
      tacctcact tacatgttc gtgcacatt ggctacttg 
      catttggtg gtgaatcta gtagtccag gtttttttg 
      gggattccc ctcgaaatg gtgcatgat tggtggcgc 
      gttctttta gtttacgtc acgggagtg gttgccggt 
      tcgctcggg acgtcaatc actgatccc agggctaag 
      ttggccggg gccagcggg ggggtttac tcgctcatc 
      acggcacat attgccacc ataattatg aattggcac 
      gagatgagc atgccgttc gtccaactg gcgattttt 
      ttgctcatt acgggggtt gatgtgggg acttcgatt 
      tataacagg tattgggcc aacttggat gagcccatt 
      gggtacatg gcgcatttt tcgggggct ttggccggg 
      cttttggtc ggtatttgt gttctcaag aatttgcaa 
      atcaccaaa aaggagaat attatttgg tgggtgtcc 
      attgcggtg tatttggcc ctgatggtg ggggccgtc 
      gtgtggaac attttctac agtgattat ttccccaca 
      cagaagtaa
    mRNA sequence
    • >TC015513 RA
      ttagtgcga taaataacc ttgacgtga ttcatcaag 
      gaaattccg gtacccact taatttttt ttctaatca 
      actatacaa caaaagtca aaggccact tctttcacc 
      agctattta aaccaaact atctagtgt aacaactga 
      cagtcgtaa taatcacaa caatgccaa aaaacctgg 
      ataaatttt tgacattcc gggaaattg gggaacgcc 
      tattgatat ctaatctta tctaaacaa aaatttgaa 
      tacgccttg agaccgcaa aaaccatgg cttcgaaca 
      ggtcgcttc acctggacc tggaattaa gaactaggg 
      atgaatccc aaattcccc tcaaccaca cccctacgt 
      cacccacgg accaatact atcgcagta tttttgaca 
      gggctgaca ctaacaagg acggggtag tgaccccca 
      acgagctcg agcgggtgg tggaccgct tgagcctca 
      ccgatgagc ccccaccgc aacaactaa tccgccaaa 
      tccaccaaa tggccgaca agaaccaag atggtggac 
      tagattatg gggaatttg tcacaatga taaaaacac 
      agcccctct ttaagaaag ttgtaaccc gttatgtga 
      atttcatcc tcccgagga aaaaacagg gggctgtgg 
      ccacccaag tcgacggtg ccggcgaat acgaagacc 
      agtacaaat tttgcccgc caaaaatcg gaatgatca 
      caatttcaa tcatcgagt tgatttttt tcgccattg 
      atgaatacc gtgaacgca actccaccc tatttgccg 
      ctggaccaa tggcccaat tgtttattt acgacccgg 
      aacggcgtt acgaggctt ggcgctacc tcacttaca 
      tgttcgtgc acattggct acttgcatt tggtggtga 
      atctagtag tccaggttt ttttgggga ttcccctcg 
      aaatggtgc atgattggt ggcgcgttc ttttagttt 
      acgtcacgg gagtggttg ccggttcgc tcgggacgt 
      caatcactg atcccaggg ctaagttgg ccggggcca 
      gcggggggg tttactcgc tcatcacgg cacatattg 
      ccaccataa ttatgaatt ggcacgaga tgagcatgc 
      cgttcgtcc aactggcga tttttttgc tcattacgg 
      gggttgatg tggggactt cgatttata acaggtatt 
      gggccaact tggatgagc ccattgggt acatggcgc 
      atttttcgg gggctttgg ccgggcttt tggtcggta 
      tttgtgttc tcaagaatt tgcaaatca ccaaaaagg 
      agaatatta tttggtggg tgtccattg cggtgtatt 
      tggccctga tggtggggg ccgtcgtgt ggaacattt 
      tctacagtg attatttcc ccacacaga agtaatttt 
      gtaccgatt tttgtaata aaacttgat caaaatc