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Gene information
General information
TC015510 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid40: 684741..686747
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015510
    iBeetle screen iB_02484
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    egg chamber number decreased - Number of animals showing the phenotype: 3
    vitellogenic egg chamber late not present - Number of animals showing the phenotype: 2

    vitellogenic egg chamber late mostlynot present - Number of animals showing the phenotype: 1

    tropharium structure irregular - Number of animals showing the phenotype: 1

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 80.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection



    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015510 RA
      MSELVSKCV LRVEVNDPS ADGGAEASS EPKTGRKSR 
      DISVETFPS RLESPREDW GLLPVCSRE TTPLEPEDP 
      KSEIGFFSG NPFVEITKG ILHLYKEDV LSDRKEALT 
      LCLLGVPTS MTCHDLLAF TAPCHADIA HIRVLRDSL 
      PNQYMALLT FRTHDSAME FYVTFNGGP FNSLEPDNI 
      CRIVWVSRV EWAHDGVPP PGHTELPIC PVCLERMDE 
      SVDGVLTIL CNHAFHANC LEQWGDSTC PVCRCVQSP 
      EQAASSECE QCGKVAQSA DALWICLIC GHVGCGRYQ 
      GGHAALHYR ESGHCYALQ LGSHRVWDY KGDNFVHRL 
      LQNKADGKL VPSEGPPSE AECAQEKVD SVQLEFTYL 
      LTSQLEEQR LYFEDKLAQ LDSIYYAEN SELKAEVLE 
      LSEKNSQLK TDIANLTKE KLALERKIA QQSVKLNAT 
      LNDLNEEKQ LGKALRNNQ QQWQTKLGE LQAEKDTMA 
      KEITELREQ VRDLMFFID AKQVIEKSD DRDDIAGGT 
      ITVGAEPAK NKRKGKKKR
    CDS sequence
    • >TC015510 RA
      atgtccgaa ttggtctcc aaatgcgtc cttcgggtg 
      gaagtgaac gacccgagc gctgatggg ggtgctgag 
      gcctcaagt gagccgaaa acggggcgc aaatcgcgc 
      gatatcagc gtggagacg ttccccagc cgcttggag 
      agccctcgg gaggactgg ggcctcctc ccggtgtgc 
      tcccgcgag accaccccc ttggagccc gaagacccc 
      aaaagcgaa attgggttt ttcagcggc aatcccttt 
      gtcgaaatt acgaaagga atcttgcat ttatataaa 
      gaagatgtt ttaagtgat aggaaggag gcgttgacg 
      ctttgtctg ctaggggtg ccaacctca atgacctgt 
      catgaccta ttggcgttc acagccccc tgtcacgcc 
      gacattgcc cacattcgg gtgctccgg gactcctta 
      cccaaccag tacatggct ctgttaact tttaggacg 
      cacgactcg gctatggag ttttacgtc acgtttaat 
      gggggcccc tttaacagt ctggaaccg gataatatc 
      tgccggatc gtttgggtg tcgcgcgtg gagtgggcc 
      cacgatggg gtgcccccg cccggccac accgagctc 
      cccatttgc cccgtctgt ctcgagcgc atggatgag 
      tcagttgac ggtgttttg acgattttg tgcaatcac 
      gcgttccac gccaactgt ctggagcag tggggcgat 
      tccacgtgc cctgtgtgc cgttgtgtc caaagcccc 
      gaacaggcg gcaagttcc gaatgtgaa cagtgcgga 
      aaagtggcc caatcagcc gacgcactt tggatttgt 
      cttatctgt ggtcacgtg ggctgtggg cggtaccaa 
      gggggccat gcggcgctc cattaccgc gaatcgggg 
      cactgttac gccctccaa ttggggtcc caccgcgtg 
      tgggactac aaaggcgat aatttcgta cataggtta 
      cttcagaat aaggcagat gggaaactg gtgccttcg 
      gaaggcccc ccttccgag gcggagtgc gcgcaagag 
      aaagtcgat tcagtgcaa ctcgaattc acctatttg 
      ttaacctcg cagttggag gagcaacgc ttgtatttc 
      gaggataaa ttagcacaa cttgatagt atttattac 
      gccgaaaat tcggaactt aaggcagag gtgctcgaa 
      ttaagcgag aaaaatagc caacttaaa acggacata 
      gctaatttg accaaggaa aagttggct ttggagcga 
      aaaatcgcc caacagagc gtcaagtta aacgcgact 
      ttaaatgat ctgaacgag gaaaaacag ctggggaaa 
      gccttgcgc aacaatcag caacaatgg caaaccaag 
      ttgggtgaa ttacaggct gagaaagac accatggca 
      aaggaaata accgaattg agggaacaa gtccgtgac 
      ttgatgttt tttattgac gctaaacaa gtcattgaa 
      aaatcggac gatcgcgat gacattgcc ggcggtacg 
      atcacagtg ggcgccgag ccggcgaaa aataaacga 
      aaaggcaag aaaaagcgt taa
    mRNA sequence
    • >TC015510 RA
      cgccactgt tgcaacaat aataccccg ttgtatacc 
      aaattttgg tgcaaaatt cacgcgaaa tgtccgaat 
      tggtctcca aatgcgtcc ttcgggtgg aagtgaacg 
      acccgagcg ctgatgggg gtgctgagg cctcaagtg 
      agccgaaaa cggggcgca aatcgcgcg atatcagcg 
      tggagacgt tccccagcc gcttggaga gccctcggg 
      aggactggg gcctcctcc cggtgtgct cccgcgaga 
      ccaccccct tggagcccg aagacccca aaagcgaaa 
      ttgggtttt tcagcggca atccctttg tcgaaatta 
      cgaaaggaa tcttgcatt tatataaag aagatgttt 
      taagtgata ggaaggagg cgttgacgc tttgtctgc 
      taggggtgc caacctcaa tgacctgtc atgacctat 
      tggcgttca cagccccct gtcacgccg acattgccc 
      acattcggg tgctccggg actccttac ccaaccagt 
      acatggctc tgttaactt ttaggacgc acgactcgg 
      ctatggagt tttacgtca cgtttaatg ggggcccct 
      ttaacagtc tggaaccgg ataatatct gccggatcg 
      tttgggtgt cgcgcgtgg agtgggccc acgatgggg 
      tgcccccgc ccggccaca ccgagctcc ccatttgcc 
      ccgtctgtc tcgagcgca tggatgagt cagttgacg 
      gtgttttga cgattttgt gcaatcacg cgttccacg 
      ccaactgtc tggagcagt ggggcgatt ccacgtgcc 
      ctgtgtgcc gttgtgtcc aaagccccg aacaggcgg 
      caagttccg aatgtgaac agtgcggaa aagtggccc 
      aatcagccg acgcacttt ggatttgtc ttatctgtg 
      gtcacgtgg gctgtgggc ggtaccaag ggggccatg 
      cggcgctcc attaccgcg aatcggggc actgttacg 
      ccctccaat tggggtccc accgcgtgt gggactaca 
      aaggcgata atttcgtac ataggttac ttcagaata 
      aggcagatg ggaaactgg tgccttcgg aaggccccc 
      cttccgagg cggagtgcg cgcaagaga aagtcgatt 
      cagtgcaac tcgaattca cctatttgt taacctcgc 
      agttggagg agcaacgct tgtatttcg aggataaat 
      tagcacaac ttgatagta tttattacg ccgaaaatt 
      cggaactta aggcagagg tgctcgaat taagcgaga 
      aaaatagcc aacttaaaa cggacatag ctaatttga 
      ccaaggaaa agttggctt tggagcgaa aaatcgccc 
      aacagagcg tcaagttaa acgcgactt taaatgatc 
      tgaacgagg aaaaacagc tggggaaag ccttgcgca 
      acaatcagc aacaatggc aaaccaagt tgggtgaat 
      tacaggctg agaaagaca ccatggcaa aggaaataa 
      ccgaattga gggaacaag tccgtgact tgatgtttt 
      ttattgacg ctaaacaag tcattgaaa aatcggacg 
      atcgcgatg acattgccg gcggtacga tcacagtgg 
      gcgccgagc cggcgaaaa ataaacgaa aaggcaaga 
      aaaagcgtt aattgtctc atgtgattg ttaatttaa 
      gtgtaatta aatatttgc aaaatca