Status
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Gene information
General information
TC015462 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 9581312..9595910
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC015462
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02482

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva dorsal not closed - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen partially constricted - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015462 RA
      MSERNGGAT VPRSDTDDV ELRNLTTDN DKRTRFQVN 
RVRSESHNT DKDGKDSVG VLTEDENTD DDDLHSVTD 
RTRLNSEYV KSFRHLTRE ALPRLDNYR NIMSLQAAN 
RPTLDELHN ATLPNKGTS VTNLNDKTG DIDGQIKFG 
WIQGVLMRC LLNIWGVML FLRLSWVVA QAGVGEAIL 
LILTTTVVT SITALSMSA ISTNGVIKG GGTYYMISR 
SLGPEFGGS IGLIFALAN AVACAMYVV GFCESLNAL 
LTTHQLFII DGSVQDVRI IGTLTIVVL TLIVVIGME 
WEAKAQIGL LVILLAAIF DFFVGSFIG PTKQESIDK 
GFVGFNLTV LRENFYPDY RKSQGVNHN FFTVFAVFF 
PAATGILAG ANISGDLKD PQKSIPKGT LLAILITTL 
SYILMAFIC GFTVLRDAT GPLTTTSVT LNLTNETLT 
NVTTINVTI PPTGPFKYG LHNDFQVVE LVAWFGPII 
YAGCFAATL SSALASLVS APKVFQALC KDKLYPGIE 
WFAKGYGKN NEPMRGYVL TFIIAVGFI LIGELNVIA 
PLISNFFLA AYTLINFST FHASLAKPV GWRPTFKYY 
NMWLSLAGS ILCVLVMFL ISWWTALVT FAAVLALYL 
IVSVRKPDV NWGSTTQAQ IYKNALQAV QQLNAVEEH 
VKNYRPQIL VLSGMPSAR PALVDFAYL ITKNQSLLV 
CGHINTSRL HQRVRNVLN YKANCWLRA HKLRAFYMQ 
VDGQNFEDG CTSLLKACG IGKLRPNIL LMGHKSDWQ 
TCSKDDLEQ YFCVLHKAL DLHLSVGIL RLQEGFDFS 
HHYADDPKI KNSTESLPR NQSYSHMSQ ASSNSDISV 
PNTPISRNR EVVNVCPSA EDSAKKPKS VGGSLSEIV 
VDDYIDRER MSQMFLFQK KQKRGVIDV WWLYDDGGL 
TLLLPYIIS TRRNWSTCK LRVFALANK RDELELEHR 
NMASLLAKF RIDYSDLQV VSDITNKPT DMTLQFFDQ 
LIADFRKPE DENDPSSVQ ITDSELMAV KDKTNRHLR 
LRELLYEHS SESQMIVMT LPIPRKGIV SAPLYLAWL 
EALTRDMPP MLLVRGNQT SVLTFYS
    CDS sequence
    • >TC015462 RA
      atgtctgaa agaaacggg ggtgccaca gtgccacga 
agtgacact gatgacgtc gaattgagg aacttgaca 
accgataat gacaaaagg acacggttt caagtgaat 
agagtacga agtgaaagc cacaacacg gataaagat 
gggaaggat tctgtcggt gttctcaca gaggatgag 
aacactgac gatgacgat ttgcactcg gtcacagat 
aggacacga ctcaactcg gagtatgtg aagagtttc 
cgacacttg acacgagag gctttgcca cgactggac 
aactacagg aacataatg tccctacag gctgccaat 
agaccaaca cttgatgaa ctccacaat gccactctt 
cccaataag ggaacgtcc gtaacaaat ttaaatgac 
aaaaccggc gatatcgac ggccaaata aaattcgga 
tggattcag ggagtttta atgcgatgt ctgctcaac 
atatggggc gttatgctg ttcctgcga ctgtcctgg 
gttgtggct caggctgga gtaggcgaa gcaattctt 
cttattttg accacaact gtggtaacg tccatcaca 
gccttgtcc atgtctgcc atcagcacc aacggtgtt 
atcaaagga ggagggacc tattatatg atctcgcgc 
agtttgggc cccgaattt ggcggttca atcggtctg 
attttcgcc ttagctaat gcagtggct tgtgctatg 
tacgttgtt ggtttttgc gaatctctc aatgcttta 
ctaacaaca caccagctt ttcatcatt gatggaagt 
gttcaagac gtgcggatt atcggcacg ctcacaatt 
gtcgtcctg acgctaatt gttgtcatt ggaatggag 
tgggaggct aaggcacaa atcggcctt cttgtgatc 
ctcctagct gcaattttt gattttttc gttggtagt 
tttatcggt ccgacgaaa caagaatcg atcgacaag 
ggatttgtc ggctttaat ttaactgtt ttacgcgag 
aatttttat ccagattat aggaaatct caaggcgtg 
aatcataat tttttcacg gtttttgcg gttttcttc 
ccggcggct accgggatt ttagctggc gccaacatc 
tcaggggac cttaaggac cctcagaag agtatccct 
aaagggacc ctgctggca attctgata accaccctg 
tcttatatt ttaatggca tttatttgt ggttttact 
gtgcttaga gacgccacc ggtcctcta accaccaca 
agtgtaaca cttaactta accaatgaa actttgaca 
aatgtaaca acaataaat gttacaatt ccgccaacg 
ggaccattt aaatatggt ctacacaat gattttcaa 
gtggtcgaa ttggttgct tggttcggt ccaattatc 
tatgctggt tgttttgct gcaacttta tcttcggca 
ctcgcttcg ctcgtttcg gcacctaaa gtgtttcag 
gctttgtgc aaagacaaa ctctatcca ggaatcgaa 
tggtttgct aaaggttat gggaaaaac aacgaaccg 
atgagagga tatgtcctt acttttatt atagccgtt 
gggtttatt ttaatcgga gaattgaac gtgattgct 
ccgcttata tcaaatttc ttcttagct gcctacact 
ttgatcaat ttctcgacg tttcatgca tctttggcc 
aaaccagtc ggctggaga cccacattt aagtactac 
aacatgtgg cttagcctc gccggttcc attctgtgc 
gtcttggtc atgtttttg atttcctgg tggacagct 
ttggtcact tttgccgca gtcctagcc ctttatttg 
atagtttcc gttcgaaaa cctgacgta aactggggc 
tcaaccaca caagcccaa atttacaaa aacgccctt 
caagccgtc cagcaattg aacgccgtt gaagaacac 
gtcaaaaat tacagacca cagattttg gttttatca 
ggaatgcca agtgcgcgg ccagcttta gtcgacttt 
gcatattta atcaccaaa aatcagtct ttgctagtg 
tgtggacac attaatacg agccgattg caccagcgg 
gtccgaaac gtcctcaac tacaaagcc aactgttgg 
ctaagggcc cacaaattg cgcgctttc tacatgcaa 
gtcgatggt caaaacttt gaagacggt tgtacttcg 
ttacttaag gcttgcggt atcggaaaa ttgcggcct 
aatatttta ctaatggga cacaagtct gactggcag 
acgtgttcc aaggacgat ctcgagcaa tatttctgt 
gttttacac aaagcgtta gatttgcat ttgagtgtt 
gggattttg cggctgcag gaaggcttc gattttagt 
catcattac gccgatgat ccgaaaatt aaaaacagc 
actgaatct ttaccacga aatcagtca tacagtcac 
atgtcgcaa gccagtagt aatagtgat atttcggtg 
cctaacact cccatctcg aggaaccgg gaagtggtt 
aatgtttgt cccagtgct gaggattcg gcaaaaaag 
ccgaaatcg gtcgggggc tctttgagc gaaatcgtc 
gttgacgat tatatcgac agggagagg atgagtcag 
atgtttctg ttccagaag aaacagaag cggggggtt 
attgacgtg tggtggctt tatgacgat ggaggcttg 
actttgctc cttccgtac ataataagc acccgaagg 
aactggagc acttgcaag ctgcgcgtg ttcgctttg 
gccaacaag cgcgacgag ctcgaacta gagcaccga 
aacatggct agtcttttg gccaagttc cgaatcgac 
tattccgat ttacaagta gtctccgac attacgaat 
aaaccaaca gacatgaca ttgcaattt ttcgaccaa 
ctgatcgcc gatttccgg aaaccggaa gacgaaaat 
gatccaagt tcggtgcaa ataactgac tcggagctg 
atggcagtc aaggacaaa actaatcgg catttgcga 
ctccgagag ctcctgtac gagcattcg tccgagtcg 
caaatgatt gtgatgact ttgcccatt ccgaggaaa 
ggcatagtg tcggcacct ctgtatttg gcctggttg 
gaagcccta actagagac atgcccccg atgctgctg 
gttaggggg aatcaaacg tccgtcctt actttttac 
tcttaa
    mRNA sequence
    • >TC015462 RA
      ctgcaacca acaccggaa gcggtgtaa acgacccat 
agcaacaaa attttcaaa acaaagcag ctggagttc 
acaacagtc aaaaacttc tttttatcc ttgattgtc 
aatacagtt accatctgg cgctttaca ccgcacaag 
ttatttgac tagggtagg gtaggacaa ggcagtgat 
gccgtatat tgcactgga tggaaatgt acaagttta 
caacctcgc attgtctgt ttcgaagtc gtactgtac 
gcctcgtgt ttcgcgtct caaatgcac gtgttttaa 
aaaatatca acgattaga aaaaaatga gtggcttgt 
gcaaaatgt gattttgtt tcggtttta acagtgtgc 
tgttttttg tctttttta acgactttt tggattatc 
tctgtacct ataagtgat tgccatgtc tgaaagaaa 
cgggggtgc cacagtgcc acgaagtga cactgatga 
cgtcgaatt gaggaactt gacaaccga taatgacaa 
aaggacacg gtttcaagt gaatagagt acgaagtga 
aagccacaa cacggataa agatgggaa ggattctgt 
cggtgttct cacagagga tgagaacac tgacgatga 
cgatttgca ctcggtcac agataggac acgactcaa 
ctcggagta tgtgaagag tttccgaca cttgacacg 
agaggcttt gccacgact ggacaacta caggaacat 
aatgtccct acaggctgc caatagacc aacacttga 
tgaactcca caatgccac tcttcccaa taagggaac 
gtccgtaac aaatttaaa tgacaaaac cggcgatat 
cgacggcca aataaaatt cggatggat tcagggagt 
tttaatgcg atgtctgct caacatatg gggcgttat 
gctgttcct gcgactgtc ctgggttgt ggctcaggc 
tggagtagg cgaagcaat tcttcttat tttgaccac 
aactgtggt aacgtccat cacagcctt gtccatgtc 
tgccatcag caccaacgg tgttatcaa aggaggagg 
gacctatta tatgatctc gcgcagttt gggccccga 
atttggcgg ttcaatcgg tctgatttt cgccttagc 
taatgcagt ggcttgtgc tatgtacgt tgttggttt 
ttgcgaatc tctcaatgc tttactaac aacacacca 
gcttttcat cattgatgg aagtgttca agacgtgcg 
gattatcgg cacgctcac aattgtcgt cctgacgct 
aattgttgt cattggaat ggagtggga ggctaaggc 
acaaatcgg ccttcttgt gatcctcct agctgcaat 
ttttgattt tttcgttgg tagttttat cggtccgac 
gaaacaaga atcgatcga caagggatt tgtcggctt 
taatttaac tgttttacg cgagaattt ttatccaga 
ttataggaa atctcaagg cgtgaatca taatttttt 
cacggtttt tgcggtttt cttcccggc ggctaccgg 
gattttagc tggcgccaa catctcagg ggaccttaa 
ggaccctca gaagagtat ccctaaagg gaccctgct 
ggcaattct gataaccac cctgtctta tattttaat 
ggcatttat ttgtggttt tactgtgct tagagacgc 
caccggtcc tctaaccac cacaagtgt aacacttaa 
cttaaccaa tgaaacttt gacaaatgt aacaacaat 
aaatgttac aattccgcc aacgggacc atttaaata 
tggtctaca caatgattt tcaagtggt cgaattggt 
tgcttggtt cggtccaat tatctatgc tggttgttt 
tgctgcaac tttatcttc ggcactcgc ttcgctcgt 
ttcggcacc taaagtgtt tcaggcttt gtgcaaaga 
caaactcta tccaggaat cgaatggtt tgctaaagg 
ttatgggaa aaacaacga accgatgag aggatatgt 
ccttacttt tattatagc cgttgggtt tattttaat 
cggagaatt gaacgtgat tgctccgct tatatcaaa 
tttcttctt agctgccta cactttgat caatttctc 
gacgtttca tgcatcttt ggccaaacc agtcggctg 
gagacccac atttaagta ctacaacat gtggcttag 
cctcgccgg ttccattct gtgcgtctt ggtcatgtt 
tttgatttc ctggtggac agctttggt cacttttgc 
cgcagtcct agcccttta tttgatagt ttccgttcg 
aaaacctga cgtaaactg gggctcaac cacacaagc 
ccaaattta caaaaacgc ccttcaagc cgtccagca 
attgaacgc cgttgaaga acacgtcaa aaattacag 
accacagat tttggtttt atcaggaat gccaagtgc 
gcggccagc tttagtcga ctttgcata tttaatcac 
caaaaatca gtctttgct agtgtgtgg acacattaa 
tacgagccg attgcacca gcgggtccg aaacgtcct 
caactacaa agccaactg ttggctaag ggcccacaa 
attgcgcgc tttctacat gcaagtcga tggtcaaaa 
ctttgaaga cggttgtac ttcgttact taaggcttg 
cggtatcgg aaaattgcg gcctaatat tttactaat 
gggacacaa gtctgactg gcagacgtg ttccaagga 
cgatctcga gcaatattt ctgtgtttt acacaaagc 
gttagattt gcatttgag tgttgggat tttgcggct 
gcaggaagg cttcgattt tagtcatca ttacgccga 
tgatccgaa aattaaaaa cagcactga atctttacc 
acgaaatca gtcatacag tcacatgtc gcaagccag 
tagtaatag tgatatttc ggtgcctaa cactcccat 
ctcgaggaa ccgggaagt ggttaatgt ttgtcccag 
tgctgagga ttcggcaaa aaagccgaa atcggtcgg 
gggctcttt gagcgaaat cgtcgttga cgattatat 
cgacaggga gaggatgag tcagatgtt tctgttcca 
gaagaaaca gaagcgggg ggttattga cgtgtggtg 
gctttatga cgatggagg cttgacttt gctccttcc 
gtacataat aagcacccg aaggaactg gagcacttg 
caagctgcg cgtgttcgc tttggccaa caagcgcga 
cgagctcga actagagca ccgaaacat ggctagtct 
tttggccaa gttccgaat cgactattc cgatttaca 
agtagtctc cgacattac gaataaacc aacagacat 
gacattgca atttttcga ccaactgat cgccgattt 
ccggaaacc ggaagacga aaatgatcc aagttcggt 
gcaaataac tgactcgga gctgatggc agtcaagga 
caaaactaa tcggcattt gcgactccg agagctcct 
gtacgagca ttcgtccga gtcgcaaat gattgtgat 
gactttgcc cattccgag gaaaggcat agtgtcggc 
acctctgta tttggcctg gttggaagc cctaactag 
agacatgcc cccgatgct gctggttag ggggaatca 
aacgtccgt ccttacttt ttactctta attttattg 
tagcgaaca atctcactt gtattgaaa atactcacc 
gaatgtcgg cggttttta ttatcaggc gttagttta 
gtatttttg aatacttta caaagtatt gtactcgta 
ttatttttt aactcgatg atgaaaagt attagtagg 
tacatgata gcttaagcc ttattttat ttaatctgt 
atttgtgcg aaaagcata ataaagttg tctttttta 
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