Status
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Gene information
General information
TC015462 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 9581312..9595910
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015462
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02482

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva dorsal not closed - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen partially constricted - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015462 RA
      MSERNGGAT VPRSDTDDV ELRNLTTDN DKRTRFQVN 
      RVRSESHNT DKDGKDSVG VLTEDENTD DDDLHSVTD 
      RTRLNSEYV KSFRHLTRE ALPRLDNYR NIMSLQAAN 
      RPTLDELHN ATLPNKGTS VTNLNDKTG DIDGQIKFG 
      WIQGVLMRC LLNIWGVML FLRLSWVVA QAGVGEAIL 
      LILTTTVVT SITALSMSA ISTNGVIKG GGTYYMISR 
      SLGPEFGGS IGLIFALAN AVACAMYVV GFCESLNAL 
      LTTHQLFII DGSVQDVRI IGTLTIVVL TLIVVIGME 
      WEAKAQIGL LVILLAAIF DFFVGSFIG PTKQESIDK 
      GFVGFNLTV LRENFYPDY RKSQGVNHN FFTVFAVFF 
      PAATGILAG ANISGDLKD PQKSIPKGT LLAILITTL 
      SYILMAFIC GFTVLRDAT GPLTTTSVT LNLTNETLT 
      NVTTINVTI PPTGPFKYG LHNDFQVVE LVAWFGPII 
      YAGCFAATL SSALASLVS APKVFQALC KDKLYPGIE 
      WFAKGYGKN NEPMRGYVL TFIIAVGFI LIGELNVIA 
      PLISNFFLA AYTLINFST FHASLAKPV GWRPTFKYY 
      NMWLSLAGS ILCVLVMFL ISWWTALVT FAAVLALYL 
      IVSVRKPDV NWGSTTQAQ IYKNALQAV QQLNAVEEH 
      VKNYRPQIL VLSGMPSAR PALVDFAYL ITKNQSLLV 
      CGHINTSRL HQRVRNVLN YKANCWLRA HKLRAFYMQ 
      VDGQNFEDG CTSLLKACG IGKLRPNIL LMGHKSDWQ 
      TCSKDDLEQ YFCVLHKAL DLHLSVGIL RLQEGFDFS 
      HHYADDPKI KNSTESLPR NQSYSHMSQ ASSNSDISV 
      PNTPISRNR EVVNVCPSA EDSAKKPKS VGGSLSEIV 
      VDDYIDRER MSQMFLFQK KQKRGVIDV WWLYDDGGL 
      TLLLPYIIS TRRNWSTCK LRVFALANK RDELELEHR 
      NMASLLAKF RIDYSDLQV VSDITNKPT DMTLQFFDQ 
      LIADFRKPE DENDPSSVQ ITDSELMAV KDKTNRHLR 
      LRELLYEHS SESQMIVMT LPIPRKGIV SAPLYLAWL 
      EALTRDMPP MLLVRGNQT SVLTFYS
    CDS sequence
    • >TC015462 RA
      atgtctgaa agaaacggg ggtgccaca gtgccacga 
      agtgacact gatgacgtc gaattgagg aacttgaca 
      accgataat gacaaaagg acacggttt caagtgaat 
      agagtacga agtgaaagc cacaacacg gataaagat 
      gggaaggat tctgtcggt gttctcaca gaggatgag 
      aacactgac gatgacgat ttgcactcg gtcacagat 
      aggacacga ctcaactcg gagtatgtg aagagtttc 
      cgacacttg acacgagag gctttgcca cgactggac 
      aactacagg aacataatg tccctacag gctgccaat 
      agaccaaca cttgatgaa ctccacaat gccactctt 
      cccaataag ggaacgtcc gtaacaaat ttaaatgac 
      aaaaccggc gatatcgac ggccaaata aaattcgga 
      tggattcag ggagtttta atgcgatgt ctgctcaac 
      atatggggc gttatgctg ttcctgcga ctgtcctgg 
      gttgtggct caggctgga gtaggcgaa gcaattctt 
      cttattttg accacaact gtggtaacg tccatcaca 
      gccttgtcc atgtctgcc atcagcacc aacggtgtt 
      atcaaagga ggagggacc tattatatg atctcgcgc 
      agtttgggc cccgaattt ggcggttca atcggtctg 
      attttcgcc ttagctaat gcagtggct tgtgctatg 
      tacgttgtt ggtttttgc gaatctctc aatgcttta 
      ctaacaaca caccagctt ttcatcatt gatggaagt 
      gttcaagac gtgcggatt atcggcacg ctcacaatt 
      gtcgtcctg acgctaatt gttgtcatt ggaatggag 
      tgggaggct aaggcacaa atcggcctt cttgtgatc 
      ctcctagct gcaattttt gattttttc gttggtagt 
      tttatcggt ccgacgaaa caagaatcg atcgacaag 
      ggatttgtc ggctttaat ttaactgtt ttacgcgag 
      aatttttat ccagattat aggaaatct caaggcgtg 
      aatcataat tttttcacg gtttttgcg gttttcttc 
      ccggcggct accgggatt ttagctggc gccaacatc 
      tcaggggac cttaaggac cctcagaag agtatccct 
      aaagggacc ctgctggca attctgata accaccctg 
      tcttatatt ttaatggca tttatttgt ggttttact 
      gtgcttaga gacgccacc ggtcctcta accaccaca 
      agtgtaaca cttaactta accaatgaa actttgaca 
      aatgtaaca acaataaat gttacaatt ccgccaacg 
      ggaccattt aaatatggt ctacacaat gattttcaa 
      gtggtcgaa ttggttgct tggttcggt ccaattatc 
      tatgctggt tgttttgct gcaacttta tcttcggca 
      ctcgcttcg ctcgtttcg gcacctaaa gtgtttcag 
      gctttgtgc aaagacaaa ctctatcca ggaatcgaa 
      tggtttgct aaaggttat gggaaaaac aacgaaccg 
      atgagagga tatgtcctt acttttatt atagccgtt 
      gggtttatt ttaatcgga gaattgaac gtgattgct 
      ccgcttata tcaaatttc ttcttagct gcctacact 
      ttgatcaat ttctcgacg tttcatgca tctttggcc 
      aaaccagtc ggctggaga cccacattt aagtactac 
      aacatgtgg cttagcctc gccggttcc attctgtgc 
      gtcttggtc atgtttttg atttcctgg tggacagct 
      ttggtcact tttgccgca gtcctagcc ctttatttg 
      atagtttcc gttcgaaaa cctgacgta aactggggc 
      tcaaccaca caagcccaa atttacaaa aacgccctt 
      caagccgtc cagcaattg aacgccgtt gaagaacac 
      gtcaaaaat tacagacca cagattttg gttttatca 
      ggaatgcca agtgcgcgg ccagcttta gtcgacttt 
      gcatattta atcaccaaa aatcagtct ttgctagtg 
      tgtggacac attaatacg agccgattg caccagcgg 
      gtccgaaac gtcctcaac tacaaagcc aactgttgg 
      ctaagggcc cacaaattg cgcgctttc tacatgcaa 
      gtcgatggt caaaacttt gaagacggt tgtacttcg 
      ttacttaag gcttgcggt atcggaaaa ttgcggcct 
      aatatttta ctaatggga cacaagtct gactggcag 
      acgtgttcc aaggacgat ctcgagcaa tatttctgt 
      gttttacac aaagcgtta gatttgcat ttgagtgtt 
      gggattttg cggctgcag gaaggcttc gattttagt 
      catcattac gccgatgat ccgaaaatt aaaaacagc 
      actgaatct ttaccacga aatcagtca tacagtcac 
      atgtcgcaa gccagtagt aatagtgat atttcggtg 
      cctaacact cccatctcg aggaaccgg gaagtggtt 
      aatgtttgt cccagtgct gaggattcg gcaaaaaag 
      ccgaaatcg gtcgggggc tctttgagc gaaatcgtc 
      gttgacgat tatatcgac agggagagg atgagtcag 
      atgtttctg ttccagaag aaacagaag cggggggtt 
      attgacgtg tggtggctt tatgacgat ggaggcttg 
      actttgctc cttccgtac ataataagc acccgaagg 
      aactggagc acttgcaag ctgcgcgtg ttcgctttg 
      gccaacaag cgcgacgag ctcgaacta gagcaccga 
      aacatggct agtcttttg gccaagttc cgaatcgac 
      tattccgat ttacaagta gtctccgac attacgaat 
      aaaccaaca gacatgaca ttgcaattt ttcgaccaa 
      ctgatcgcc gatttccgg aaaccggaa gacgaaaat 
      gatccaagt tcggtgcaa ataactgac tcggagctg 
      atggcagtc aaggacaaa actaatcgg catttgcga 
      ctccgagag ctcctgtac gagcattcg tccgagtcg 
      caaatgatt gtgatgact ttgcccatt ccgaggaaa 
      ggcatagtg tcggcacct ctgtatttg gcctggttg 
      gaagcccta actagagac atgcccccg atgctgctg 
      gttaggggg aatcaaacg tccgtcctt actttttac 
      tcttaa
    mRNA sequence
    • >TC015462 RA
      ctgcaacca acaccggaa gcggtgtaa acgacccat 
      agcaacaaa attttcaaa acaaagcag ctggagttc 
      acaacagtc aaaaacttc tttttatcc ttgattgtc 
      aatacagtt accatctgg cgctttaca ccgcacaag 
      ttatttgac tagggtagg gtaggacaa ggcagtgat 
      gccgtatat tgcactgga tggaaatgt acaagttta 
      caacctcgc attgtctgt ttcgaagtc gtactgtac 
      gcctcgtgt ttcgcgtct caaatgcac gtgttttaa 
      aaaatatca acgattaga aaaaaatga gtggcttgt 
      gcaaaatgt gattttgtt tcggtttta acagtgtgc 
      tgttttttg tctttttta acgactttt tggattatc 
      tctgtacct ataagtgat tgccatgtc tgaaagaaa 
      cgggggtgc cacagtgcc acgaagtga cactgatga 
      cgtcgaatt gaggaactt gacaaccga taatgacaa 
      aaggacacg gtttcaagt gaatagagt acgaagtga 
      aagccacaa cacggataa agatgggaa ggattctgt 
      cggtgttct cacagagga tgagaacac tgacgatga 
      cgatttgca ctcggtcac agataggac acgactcaa 
      ctcggagta tgtgaagag tttccgaca cttgacacg 
      agaggcttt gccacgact ggacaacta caggaacat 
      aatgtccct acaggctgc caatagacc aacacttga 
      tgaactcca caatgccac tcttcccaa taagggaac 
      gtccgtaac aaatttaaa tgacaaaac cggcgatat 
      cgacggcca aataaaatt cggatggat tcagggagt 
      tttaatgcg atgtctgct caacatatg gggcgttat 
      gctgttcct gcgactgtc ctgggttgt ggctcaggc 
      tggagtagg cgaagcaat tcttcttat tttgaccac 
      aactgtggt aacgtccat cacagcctt gtccatgtc 
      tgccatcag caccaacgg tgttatcaa aggaggagg 
      gacctatta tatgatctc gcgcagttt gggccccga 
      atttggcgg ttcaatcgg tctgatttt cgccttagc 
      taatgcagt ggcttgtgc tatgtacgt tgttggttt 
      ttgcgaatc tctcaatgc tttactaac aacacacca 
      gcttttcat cattgatgg aagtgttca agacgtgcg 
      gattatcgg cacgctcac aattgtcgt cctgacgct 
      aattgttgt cattggaat ggagtggga ggctaaggc 
      acaaatcgg ccttcttgt gatcctcct agctgcaat 
      ttttgattt tttcgttgg tagttttat cggtccgac 
      gaaacaaga atcgatcga caagggatt tgtcggctt 
      taatttaac tgttttacg cgagaattt ttatccaga 
      ttataggaa atctcaagg cgtgaatca taatttttt 
      cacggtttt tgcggtttt cttcccggc ggctaccgg 
      gattttagc tggcgccaa catctcagg ggaccttaa 
      ggaccctca gaagagtat ccctaaagg gaccctgct 
      ggcaattct gataaccac cctgtctta tattttaat 
      ggcatttat ttgtggttt tactgtgct tagagacgc 
      caccggtcc tctaaccac cacaagtgt aacacttaa 
      cttaaccaa tgaaacttt gacaaatgt aacaacaat 
      aaatgttac aattccgcc aacgggacc atttaaata 
      tggtctaca caatgattt tcaagtggt cgaattggt 
      tgcttggtt cggtccaat tatctatgc tggttgttt 
      tgctgcaac tttatcttc ggcactcgc ttcgctcgt 
      ttcggcacc taaagtgtt tcaggcttt gtgcaaaga 
      caaactcta tccaggaat cgaatggtt tgctaaagg 
      ttatgggaa aaacaacga accgatgag aggatatgt 
      ccttacttt tattatagc cgttgggtt tattttaat 
      cggagaatt gaacgtgat tgctccgct tatatcaaa 
      tttcttctt agctgccta cactttgat caatttctc 
      gacgtttca tgcatcttt ggccaaacc agtcggctg 
      gagacccac atttaagta ctacaacat gtggcttag 
      cctcgccgg ttccattct gtgcgtctt ggtcatgtt 
      tttgatttc ctggtggac agctttggt cacttttgc 
      cgcagtcct agcccttta tttgatagt ttccgttcg 
      aaaacctga cgtaaactg gggctcaac cacacaagc 
      ccaaattta caaaaacgc ccttcaagc cgtccagca 
      attgaacgc cgttgaaga acacgtcaa aaattacag 
      accacagat tttggtttt atcaggaat gccaagtgc 
      gcggccagc tttagtcga ctttgcata tttaatcac 
      caaaaatca gtctttgct agtgtgtgg acacattaa 
      tacgagccg attgcacca gcgggtccg aaacgtcct 
      caactacaa agccaactg ttggctaag ggcccacaa 
      attgcgcgc tttctacat gcaagtcga tggtcaaaa 
      ctttgaaga cggttgtac ttcgttact taaggcttg 
      cggtatcgg aaaattgcg gcctaatat tttactaat 
      gggacacaa gtctgactg gcagacgtg ttccaagga 
      cgatctcga gcaatattt ctgtgtttt acacaaagc 
      gttagattt gcatttgag tgttgggat tttgcggct 
      gcaggaagg cttcgattt tagtcatca ttacgccga 
      tgatccgaa aattaaaaa cagcactga atctttacc 
      acgaaatca gtcatacag tcacatgtc gcaagccag 
      tagtaatag tgatatttc ggtgcctaa cactcccat 
      ctcgaggaa ccgggaagt ggttaatgt ttgtcccag 
      tgctgagga ttcggcaaa aaagccgaa atcggtcgg 
      gggctcttt gagcgaaat cgtcgttga cgattatat 
      cgacaggga gaggatgag tcagatgtt tctgttcca 
      gaagaaaca gaagcgggg ggttattga cgtgtggtg 
      gctttatga cgatggagg cttgacttt gctccttcc 
      gtacataat aagcacccg aaggaactg gagcacttg 
      caagctgcg cgtgttcgc tttggccaa caagcgcga 
      cgagctcga actagagca ccgaaacat ggctagtct 
      tttggccaa gttccgaat cgactattc cgatttaca 
      agtagtctc cgacattac gaataaacc aacagacat 
      gacattgca atttttcga ccaactgat cgccgattt 
      ccggaaacc ggaagacga aaatgatcc aagttcggt 
      gcaaataac tgactcgga gctgatggc agtcaagga 
      caaaactaa tcggcattt gcgactccg agagctcct 
      gtacgagca ttcgtccga gtcgcaaat gattgtgat 
      gactttgcc cattccgag gaaaggcat agtgtcggc 
      acctctgta tttggcctg gttggaagc cctaactag 
      agacatgcc cccgatgct gctggttag ggggaatca 
      aacgtccgt ccttacttt ttactctta attttattg 
      tagcgaaca atctcactt gtattgaaa atactcacc 
      gaatgtcgg cggttttta ttatcaggc gttagttta 
      gtatttttg aatacttta caaagtatt gtactcgta 
      ttatttttt aactcgatg atgaaaagt attagtagg 
      tacatgata gcttaagcc ttattttat ttaatctgt 
      atttgtgcg aaaagcata ataaagttg tctttttta 
      a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY