General information | |||
TC015462 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 9581312..9595910 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC015462 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_02482 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
remarks: muscle pattern potentially obscured by segmentation defect,
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
larva dorsal not closed - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head capsule size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen partially constricted - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
- >TC015462 RA
MSERNGGAT VPRSDTDDV ELRNLTTDN DKRTRFQVN RVRSESHNT DKDGKDSVG VLTEDENTD DDDLHSVTD RTRLNSEYV KSFRHLTRE ALPRLDNYR NIMSLQAAN RPTLDELHN ATLPNKGTS VTNLNDKTG DIDGQIKFG WIQGVLMRC LLNIWGVML FLRLSWVVA QAGVGEAIL LILTTTVVT SITALSMSA ISTNGVIKG GGTYYMISR SLGPEFGGS IGLIFALAN AVACAMYVV GFCESLNAL LTTHQLFII DGSVQDVRI IGTLTIVVL TLIVVIGME WEAKAQIGL LVILLAAIF DFFVGSFIG PTKQESIDK GFVGFNLTV LRENFYPDY RKSQGVNHN FFTVFAVFF PAATGILAG ANISGDLKD PQKSIPKGT LLAILITTL SYILMAFIC GFTVLRDAT GPLTTTSVT LNLTNETLT NVTTINVTI PPTGPFKYG LHNDFQVVE LVAWFGPII YAGCFAATL SSALASLVS APKVFQALC KDKLYPGIE WFAKGYGKN NEPMRGYVL TFIIAVGFI LIGELNVIA PLISNFFLA AYTLINFST FHASLAKPV GWRPTFKYY NMWLSLAGS ILCVLVMFL ISWWTALVT FAAVLALYL IVSVRKPDV NWGSTTQAQ IYKNALQAV QQLNAVEEH VKNYRPQIL VLSGMPSAR PALVDFAYL ITKNQSLLV CGHINTSRL HQRVRNVLN YKANCWLRA HKLRAFYMQ VDGQNFEDG CTSLLKACG IGKLRPNIL LMGHKSDWQ TCSKDDLEQ YFCVLHKAL DLHLSVGIL RLQEGFDFS HHYADDPKI KNSTESLPR NQSYSHMSQ ASSNSDISV PNTPISRNR EVVNVCPSA EDSAKKPKS VGGSLSEIV VDDYIDRER MSQMFLFQK KQKRGVIDV WWLYDDGGL TLLLPYIIS TRRNWSTCK LRVFALANK RDELELEHR NMASLLAKF RIDYSDLQV VSDITNKPT DMTLQFFDQ LIADFRKPE DENDPSSVQ ITDSELMAV KDKTNRHLR LRELLYEHS SESQMIVMT LPIPRKGIV SAPLYLAWL EALTRDMPP MLLVRGNQT SVLTFYS
- >TC015462 RA
atgtctgaa agaaacggg ggtgccaca gtgccacga agtgacact gatgacgtc gaattgagg aacttgaca accgataat gacaaaagg acacggttt caagtgaat agagtacga agtgaaagc cacaacacg gataaagat gggaaggat tctgtcggt gttctcaca gaggatgag aacactgac gatgacgat ttgcactcg gtcacagat aggacacga ctcaactcg gagtatgtg aagagtttc cgacacttg acacgagag gctttgcca cgactggac aactacagg aacataatg tccctacag gctgccaat agaccaaca cttgatgaa ctccacaat gccactctt cccaataag ggaacgtcc gtaacaaat ttaaatgac aaaaccggc gatatcgac ggccaaata aaattcgga tggattcag ggagtttta atgcgatgt ctgctcaac atatggggc gttatgctg ttcctgcga ctgtcctgg gttgtggct caggctgga gtaggcgaa gcaattctt cttattttg accacaact gtggtaacg tccatcaca gccttgtcc atgtctgcc atcagcacc aacggtgtt atcaaagga ggagggacc tattatatg atctcgcgc agtttgggc cccgaattt ggcggttca atcggtctg attttcgcc ttagctaat gcagtggct tgtgctatg tacgttgtt ggtttttgc gaatctctc aatgcttta ctaacaaca caccagctt ttcatcatt gatggaagt gttcaagac gtgcggatt atcggcacg ctcacaatt gtcgtcctg acgctaatt gttgtcatt ggaatggag tgggaggct aaggcacaa atcggcctt cttgtgatc ctcctagct gcaattttt gattttttc gttggtagt tttatcggt ccgacgaaa caagaatcg atcgacaag ggatttgtc ggctttaat ttaactgtt ttacgcgag aatttttat ccagattat aggaaatct caaggcgtg aatcataat tttttcacg gtttttgcg gttttcttc ccggcggct accgggatt ttagctggc gccaacatc tcaggggac cttaaggac cctcagaag agtatccct aaagggacc ctgctggca attctgata accaccctg tcttatatt ttaatggca tttatttgt ggttttact gtgcttaga gacgccacc ggtcctcta accaccaca agtgtaaca cttaactta accaatgaa actttgaca aatgtaaca acaataaat gttacaatt ccgccaacg ggaccattt aaatatggt ctacacaat gattttcaa gtggtcgaa ttggttgct tggttcggt ccaattatc tatgctggt tgttttgct gcaacttta tcttcggca ctcgcttcg ctcgtttcg gcacctaaa gtgtttcag gctttgtgc aaagacaaa ctctatcca ggaatcgaa tggtttgct aaaggttat gggaaaaac aacgaaccg atgagagga tatgtcctt acttttatt atagccgtt gggtttatt ttaatcgga gaattgaac gtgattgct ccgcttata tcaaatttc ttcttagct gcctacact ttgatcaat ttctcgacg tttcatgca tctttggcc aaaccagtc ggctggaga cccacattt aagtactac aacatgtgg cttagcctc gccggttcc attctgtgc gtcttggtc atgtttttg atttcctgg tggacagct ttggtcact tttgccgca gtcctagcc ctttatttg atagtttcc gttcgaaaa cctgacgta aactggggc tcaaccaca caagcccaa atttacaaa aacgccctt caagccgtc cagcaattg aacgccgtt gaagaacac gtcaaaaat tacagacca cagattttg gttttatca ggaatgcca agtgcgcgg ccagcttta gtcgacttt gcatattta atcaccaaa aatcagtct ttgctagtg tgtggacac attaatacg agccgattg caccagcgg gtccgaaac gtcctcaac tacaaagcc aactgttgg ctaagggcc cacaaattg cgcgctttc tacatgcaa gtcgatggt caaaacttt gaagacggt tgtacttcg ttacttaag gcttgcggt atcggaaaa ttgcggcct aatatttta ctaatggga cacaagtct gactggcag acgtgttcc aaggacgat ctcgagcaa tatttctgt gttttacac aaagcgtta gatttgcat ttgagtgtt gggattttg cggctgcag gaaggcttc gattttagt catcattac gccgatgat ccgaaaatt aaaaacagc actgaatct ttaccacga aatcagtca tacagtcac atgtcgcaa gccagtagt aatagtgat atttcggtg cctaacact cccatctcg aggaaccgg gaagtggtt aatgtttgt cccagtgct gaggattcg gcaaaaaag ccgaaatcg gtcgggggc tctttgagc gaaatcgtc gttgacgat tatatcgac agggagagg atgagtcag atgtttctg ttccagaag aaacagaag cggggggtt attgacgtg tggtggctt tatgacgat ggaggcttg actttgctc cttccgtac ataataagc acccgaagg aactggagc acttgcaag ctgcgcgtg ttcgctttg gccaacaag cgcgacgag ctcgaacta gagcaccga aacatggct agtcttttg gccaagttc cgaatcgac tattccgat ttacaagta gtctccgac attacgaat aaaccaaca gacatgaca ttgcaattt ttcgaccaa ctgatcgcc gatttccgg aaaccggaa gacgaaaat gatccaagt tcggtgcaa ataactgac tcggagctg atggcagtc aaggacaaa actaatcgg catttgcga ctccgagag ctcctgtac gagcattcg tccgagtcg caaatgatt gtgatgact ttgcccatt ccgaggaaa ggcatagtg tcggcacct ctgtatttg gcctggttg gaagcccta actagagac atgcccccg atgctgctg gttaggggg aatcaaacg tccgtcctt actttttac tcttaa
- >TC015462 RA
ctgcaacca acaccggaa gcggtgtaa acgacccat agcaacaaa attttcaaa acaaagcag ctggagttc acaacagtc aaaaacttc tttttatcc ttgattgtc aatacagtt accatctgg cgctttaca ccgcacaag ttatttgac tagggtagg gtaggacaa ggcagtgat gccgtatat tgcactgga tggaaatgt acaagttta caacctcgc attgtctgt ttcgaagtc gtactgtac gcctcgtgt ttcgcgtct caaatgcac gtgttttaa aaaatatca acgattaga aaaaaatga gtggcttgt gcaaaatgt gattttgtt tcggtttta acagtgtgc tgttttttg tctttttta acgactttt tggattatc tctgtacct ataagtgat tgccatgtc tgaaagaaa cgggggtgc cacagtgcc acgaagtga cactgatga cgtcgaatt gaggaactt gacaaccga taatgacaa aaggacacg gtttcaagt gaatagagt acgaagtga aagccacaa cacggataa agatgggaa ggattctgt cggtgttct cacagagga tgagaacac tgacgatga cgatttgca ctcggtcac agataggac acgactcaa ctcggagta tgtgaagag tttccgaca cttgacacg agaggcttt gccacgact ggacaacta caggaacat aatgtccct acaggctgc caatagacc aacacttga tgaactcca caatgccac tcttcccaa taagggaac gtccgtaac aaatttaaa tgacaaaac cggcgatat cgacggcca aataaaatt cggatggat tcagggagt tttaatgcg atgtctgct caacatatg gggcgttat gctgttcct gcgactgtc ctgggttgt ggctcaggc tggagtagg cgaagcaat tcttcttat tttgaccac aactgtggt aacgtccat cacagcctt gtccatgtc tgccatcag caccaacgg tgttatcaa aggaggagg gacctatta tatgatctc gcgcagttt gggccccga atttggcgg ttcaatcgg tctgatttt cgccttagc taatgcagt ggcttgtgc tatgtacgt tgttggttt ttgcgaatc tctcaatgc tttactaac aacacacca gcttttcat cattgatgg aagtgttca agacgtgcg gattatcgg cacgctcac aattgtcgt cctgacgct aattgttgt cattggaat ggagtggga ggctaaggc acaaatcgg ccttcttgt gatcctcct agctgcaat ttttgattt tttcgttgg tagttttat cggtccgac gaaacaaga atcgatcga caagggatt tgtcggctt taatttaac tgttttacg cgagaattt ttatccaga ttataggaa atctcaagg cgtgaatca taatttttt cacggtttt tgcggtttt cttcccggc ggctaccgg gattttagc tggcgccaa catctcagg ggaccttaa ggaccctca gaagagtat ccctaaagg gaccctgct ggcaattct gataaccac cctgtctta tattttaat ggcatttat ttgtggttt tactgtgct tagagacgc caccggtcc tctaaccac cacaagtgt aacacttaa cttaaccaa tgaaacttt gacaaatgt aacaacaat aaatgttac aattccgcc aacgggacc atttaaata tggtctaca caatgattt tcaagtggt cgaattggt tgcttggtt cggtccaat tatctatgc tggttgttt tgctgcaac tttatcttc ggcactcgc ttcgctcgt ttcggcacc taaagtgtt tcaggcttt gtgcaaaga caaactcta tccaggaat cgaatggtt tgctaaagg ttatgggaa aaacaacga accgatgag aggatatgt ccttacttt tattatagc cgttgggtt tattttaat cggagaatt gaacgtgat tgctccgct tatatcaaa tttcttctt agctgccta cactttgat caatttctc gacgtttca tgcatcttt ggccaaacc agtcggctg gagacccac atttaagta ctacaacat gtggcttag cctcgccgg ttccattct gtgcgtctt ggtcatgtt tttgatttc ctggtggac agctttggt cacttttgc cgcagtcct agcccttta tttgatagt ttccgttcg aaaacctga cgtaaactg gggctcaac cacacaagc ccaaattta caaaaacgc ccttcaagc cgtccagca attgaacgc cgttgaaga acacgtcaa aaattacag accacagat tttggtttt atcaggaat gccaagtgc gcggccagc tttagtcga ctttgcata tttaatcac caaaaatca gtctttgct agtgtgtgg acacattaa tacgagccg attgcacca gcgggtccg aaacgtcct caactacaa agccaactg ttggctaag ggcccacaa attgcgcgc tttctacat gcaagtcga tggtcaaaa ctttgaaga cggttgtac ttcgttact taaggcttg cggtatcgg aaaattgcg gcctaatat tttactaat gggacacaa gtctgactg gcagacgtg ttccaagga cgatctcga gcaatattt ctgtgtttt acacaaagc gttagattt gcatttgag tgttgggat tttgcggct gcaggaagg cttcgattt tagtcatca ttacgccga tgatccgaa aattaaaaa cagcactga atctttacc acgaaatca gtcatacag tcacatgtc gcaagccag tagtaatag tgatatttc ggtgcctaa cactcccat ctcgaggaa ccgggaagt ggttaatgt ttgtcccag tgctgagga ttcggcaaa aaagccgaa atcggtcgg gggctcttt gagcgaaat cgtcgttga cgattatat cgacaggga gaggatgag tcagatgtt tctgttcca gaagaaaca gaagcgggg ggttattga cgtgtggtg gctttatga cgatggagg cttgacttt gctccttcc gtacataat aagcacccg aaggaactg gagcacttg caagctgcg cgtgttcgc tttggccaa caagcgcga cgagctcga actagagca ccgaaacat ggctagtct tttggccaa gttccgaat cgactattc cgatttaca agtagtctc cgacattac gaataaacc aacagacat gacattgca atttttcga ccaactgat cgccgattt ccggaaacc ggaagacga aaatgatcc aagttcggt gcaaataac tgactcgga gctgatggc agtcaagga caaaactaa tcggcattt gcgactccg agagctcct gtacgagca ttcgtccga gtcgcaaat gattgtgat gactttgcc cattccgag gaaaggcat agtgtcggc acctctgta tttggcctg gttggaagc cctaactag agacatgcc cccgatgct gctggttag ggggaatca aacgtccgt ccttacttt ttactctta attttattg tagcgaaca atctcactt gtattgaaa atactcacc gaatgtcgg cggttttta ttatcaggc gttagttta gtatttttg aatacttta caaagtatt gtactcgta ttatttttt aactcgatg atgaaaagt attagtagg tacatgata gcttaagcc ttattttat ttaatctgt atttgtgcg aaaagcata ataaagttg tctttttta a
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
