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Gene information
General information
TC015454 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 9643374..9649603
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015454
    iBeetle screen iB_02481

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection



    larva lethality late (L7) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015454 RA
      MFACAILIA PAARSALIS NSKVYLWPL STALSQNPS 
      LVQSPVVQQ HKQATLLPA VRSFQTTPV SRDIDSADK 
      FIGAGAATV GVAGSGSLF IMEDNPANC LSSTLSPDF 
      EKVLKSDTF VDKSGLMLA VFETDVQPM VLCTAPRRF 
      GKTVNLSMM QRFLELEVD ENGEEKTTV LEEKDNYKL 
      FSSARFNLK VMENKKFVE THFGQYPII KITFLCCVN 
      VKSQSDAVN VCRSAISRA FRQYSYLYK SKLSPLEDD 
      EKEVCEKWC DEFTYTGIP DDKVIEGLG CLAVYLNKF 
      HKRMVFLLV DEYDSIISN ALYNVQNAD PKAELKELE 
      EAASLPIQV IGYALKGFP EIFKGAFIT GILDVGGVA 
      TSTLLGNVL RRQFGTNNL CQDFYGFTE DEVCTLFNR 
      FAVKEELRE KAKAKYNGY FNGSKQCLY NPQSIVQFL 
      GNLSLGEGA LKNYWQESG YIHNMYKLF EEGSVRTLL 
      YELLQGVKE DFQFKLRPL DLRDLKSLH NILVNQNLT 
      CREEIVVRF LVSLGYLTY SFHDRKLQI PNAELIDEI 
      MELLRDYFF ERFGINSEL AKACSASFS ELTILCDEG 
      DSLAMFKEE LANFQNSIT CLLQRSTLI DPLNEATLQ 
      SIITLTCSQ AGFLFGNEI IIPNHRTKV DIVLFNDKS 
      GIIMELKCD PNTVQDAFK EIFQKRYYN VFTNKKYFD 
      ERKKSPSLS FVMAMHVDS DNKGSVMCL RREDVIHVL 
      DDLQVDARK SSLEDRIEI AVNSMGERF INSCCEKAI 
      LVS
    CDS sequence
    • >TC015454 RA
      atgttcgcc tgcgctata ttgatcgcc cccgccgcc 
      aggagcgcc cttatttca aactctaaa gtatactta 
      tggccattg agcaccgcc ctcagccaa aacccatca 
      ttagtccag agtcctgtc gttcagcaa cacaaacaa 
      gccacactt ctcccagca gtgaggagt ttccagacc 
      accccagtg tccagggac atcgattca gctgataaa 
      tttattggt gcaggcgct gccacagta ggagtagct 
      ggatcaggc agtttgttt attatggag gataatccg 
      gccaattgt ttatcatct accctttca ccggatttt 
      gaaaaagtc ttgaaatcc gatactttt gttgataaa 
      agtgggttg atgttagca gtttttgaa acggacgtt 
      caacctatg gtattgtgt accgcaccg cgccgattt 
      ggcaaaact gtaaactta tccatgatg caacgattc 
      ttggagttg gaagtagat gaaaatgga gaggagaaa 
      acaacggtt ttggaagaa aaagacaat tacaaactc 
      ttcagttca gcaaggttc aacttaaaa gtcatggag 
      aataagaag tttgtagaa actcatttc ggacaatat 
      ccgataatc aaaataact tttctctgt tgtgttaac 
      gtgaaaagc caatctgat gcagtgaat gtgtgtaga 
      agcgctata tcgcgggct tttaggcaa tacagttac 
      ctttacaaa tccaaattg tcaccgttg gaagacgat 
      gaaaaagag gtttgcgaa aaatggtgt gacgaattt 
      acctacaca ggaattcca gatgacaaa gtaatcgag 
      gggttggga tgcttggca gtttatctg aacaagttt 
      cataaaaga atggttttt ctactcgtt gacgagtac 
      gactcgatc atttcaaat gcgttgtat aatgtgcag 
      aacgctgat ccaaaggct gagctcaaa gagctagag 
      gaagctgcc tcactccca attcaagta attggatat 
      gctttaaaa ggatttcct gagatcttt aaaggagcg 
      tttattacc ggaattttg gatgtgggt ggtgtagcg 
      acttccacc ctattgggt aatgtattg cgacggcaa 
      ttcggaacc aacaatctg tgccaagat ttctatgga 
      ttcactgaa gatgaagtt tgtactcta tttaatcgg 
      tttgcagta aaggaggaa ttgagagaa aaagcgaag 
      gctaaatat aatgggtat ttcaatggc tcgaaacaa 
      tgcctatat aatcctcaa tcgattgtg cagttcttg 
      ggaaattta tctctggga gaaggtgct ttaaaaaat 
      tattggcaa gaatctggt tacattcac aatatgtat 
      aaactattt gaagagggt agcgtgaga acgttgttg 
      tatgaactt ctacaaggt gttaaagaa gattttcaa 
      ttcaagtta agaccgtta gacttaaga gatctgaaa 
      tctttgcat aacatatta gttaatcaa aatttgact 
      tgtcgcgaa gaaatagtt gttcgattt cttgtatct 
      ctaggatac ttaacatat tcatttcat gatcgcaaa 
      ctccaaata cctaacgct gagctaatt gatgaaatt 
      atggagctg ctacgagat tattttttt gaaagattt 
      ggtattaac agtgaattg gctaaagct tgttcagca 
      tcattttct gagctaacc attctctgt gatgaaggt 
      gattcatta gcaatgttc aaagaagaa ttagcaaat 
      tttcaaaat agtataacc tgtctactt caacgttca 
      acacttatc gatccattg aatgaggca actttacag 
      agtataatt acattgaca tgtagtcaa gcaggattt 
      ctcttcgga aacgaaata ataattcca aatcataga 
      accaaagtt gatatagta ctgtttaat gataaatct 
      gggataata atggaatta aaatgtgat ccaaatact 
      gtacaagac gcatttaaa gaaatcttt caaaagcgg 
      tactacaat gttttcaca aataagaag tattttgat 
      gaacgtaag aaatctcct tctctttct tttgttatg 
      gcaatgcat gtggattct gacaacaaa ggatctgtg 
      atgtgcctg cgacgcgag gatgttatt cacgttctg 
      gatgatctg caagttgac gctagaaag tcatcgcta 
      gaagatcgc attgagata gctgttaat tctatgggt 
      gaacgtttc attaacagt tgttgcgaa aaagcaatt 
      cttgtctct taa
    mRNA sequence
    • >TC015454 RA
      ctggtggtc actccgaag tacacattc caagtgctg 
      ggttacctc ctcccgtca ctgtgccgt ggtgagtcg 
      ccgttaagt tgttaagtg tattccaac cgactaaat 
      ttaattaaa taaaatgtt cgcctgcgc tatattgat 
      cgcccccgc cgccaggag cgcccttat ttcaaactc 
      taaagtata cttatggcc attgagcac cgccctcag 
      ccaaaaccc atcattagt ccagagtcc tgtcgttca 
      gcaacacaa acaagccac acttctccc agcagtgag 
      gagtttcca gaccacccc agtgtccag ggacatcga 
      ttcagctga taaatttat tggtgcagg cgctgccac 
      agtaggagt agctggatc aggcagttt gtttattat 
      ggaggataa tccggccaa ttgtttatc atctaccct 
      ttcaccgga ttttgaaaa agtcttgaa atccgatac 
      ttttgttga taaaagtgg gttgatgtt agcagtttt 
      tgaaacgga cgttcaacc tatggtatt gtgtaccgc 
      accgcgccg atttggcaa aactgtaaa cttatccat 
      gatgcaacg attcttgga gttggaagt agatgaaaa 
      tggagagga gaaaacaac ggttttgga agaaaaaga 
      caattacaa actcttcag ttcagcaag gttcaactt 
      aaaagtcat ggagaataa gaagtttgt agaaactca 
      tttcggaca atatccgat aatcaaaat aacttttct 
      ctgttgtgt taacgtgaa aagccaatc tgatgcagt 
      gaatgtgtg tagaagcgc tatatcgcg ggcttttag 
      gcaatacag ttaccttta caaatccaa attgtcacc 
      gttggaaga cgatgaaaa agaggtttg cgaaaaatg 
      gtgtgacga atttaccta cacaggaat tccagatga 
      caaagtaat cgaggggtt gggatgctt ggcagttta 
      tctgaacaa gtttcataa aagaatggt ttttctact 
      cgttgacga gtacgactc gatcatttc aaatgcgtt 
      gtataatgt gcagaacgc tgatccaaa ggctgagct 
      caaagagct agaggaagc tgcctcact cccaattca 
      agtaattgg atatgcttt aaaaggatt tcctgagat 
      ctttaaagg agcgtttat taccggaat tttggatgt 
      gggtggtgt agcgacttc caccctatt gggtaatgt 
      attgcgacg gcaattcgg aaccaacaa tctgtgcca 
      agatttcta tggattcac tgaagatga agtttgtac 
      tctatttaa tcggtttgc agtaaagga ggaattgag 
      agaaaaagc gaaggctaa atataatgg gtatttcaa 
      tggctcgaa acaatgcct atataatcc tcaatcgat 
      tgtgcagtt cttgggaaa tttatctct gggagaagg 
      tgctttaaa aaattattg gcaagaatc tggttacat 
      tcacaatat gtataaact atttgaaga gggtagcgt 
      gagaacgtt gttgtatga acttctaca aggtgttaa 
      agaagattt tcaattcaa gttaagacc gttagactt 
      aagagatct gaaatcttt gcataacat attagttaa 
      tcaaaattt gacttgtcg cgaagaaat agttgttcg 
      atttcttgt atctctagg atacttaac atattcatt 
      tcatgatcg caaactcca aatacctaa cgctgagct 
      aattgatga aattatgga gctgctacg agattattt 
      ttttgaaag atttggtat taacagtga attggctaa 
      agcttgttc agcatcatt ttctgagct aaccattct 
      ctgtgatga aggtgattc attagcaat gttcaaaga 
      agaattagc aaattttca aaatagtat aacctgtct 
      acttcaacg ttcaacact tatcgatcc attgaatga 
      ggcaacttt acagagtat aattacatt gacatgtag 
      tcaagcagg atttctctt cggaaacga aataataat 
      tccaaatca tagaaccaa agttgatat agtactgtt 
      taatgataa atctgggat aataatgga attaaaatg 
      tgatccaaa tactgtaca agacgcatt taaagaaat 
      ctttcaaaa gcggtacta caatgtttt cacaaataa 
      gaagtattt tgatgaacg taagaaatc tccttctct 
      ttcttttgt tatggcaat gcatgtgga ttctgacaa 
      caaaggatc tgtgatgtg cctgcgacg cgaggatgt 
      tattcacgt tctggatga tctgcaagt tgacgctag 
      aaagtcatc gctagaaga tcgcattga gatagctgt 
      taattctat gggtgaacg tttcattaa cagttgttg 
      cgaaaaagc aattcttgt ctcttaaca taccagaga 
      aaccggttc agtttttgt ttaaattta aagtagcta 
      tttatatat agttataat aaaacatga attacaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY