Status
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Gene information
General information
TC015453 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 9657551..9663313
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015453
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02480

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 6 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015453 RA
      MSRFFAGSD SDSDSSSEE EPIQRAPAP VFTFSDDEE 
      EVKRVVRSA KEKRYEELT NIIKQIRNY KKIKDMSSM 
      LTSFEDLQK AYVKALPVI AKEESGQTP RFFTRCLVE 
      MEDFINEMW EDRDGRKNM SKNNSKSLA SMRQKLRKY 
      IKDFEEDIA KFRENPDQP DDEEEEEKH EPEPDTQTE 
      APAKAVAKI SKQIVDDEG SSDSDDWPS DSDSSSDSF 
      DEDEGKGTM RERFLKKTG PAHEDDEER DRRKEERRK 
      ERKEKERRK VKKEDEEDG EGEWEFVKG GIAIPSEKP 
      KMFAKDAEI DLNLVIKKL SEIMAARGK KRTDRREQI 
      ELLHELQNV SDQHQLGPA IYVKIKFAI ISAIFDYNP 
      KVSDAMKPE YWMKLLERM SEMLDLLLS NPQLFVSES 
      VTEETENLE EPPYKIRGC VLTSVERLD DEFTKLLKE 
      CDPHSNEYV ERLKDEAKV SEIIDKTMK YLERTNAPS 
      ELCRIYLRK IEHLYYKFD PRVLQQKAG EIPRDLYTS 
      VQEMEKLCK FIYAKDGTD RLRTRAILS HIYHHALHD 
      NWFQARDLV LMSHLQETI HHSDPSTQI LYNRTMAHL 
      GLCAFRHAN IKDAHNCLV DLMMTGKPK ELLAQGLLP 
      QRQHERSKE QEKIEKQRQ MPFHMHINL ELLECVYLV 
      SAMLIEIPY MAAHEFDAR RRMISKTFY QQLRSSERQ 
      SLVGPPESM REHVVAAAK AMRNGNWSA CNGFIINEK 
      MNAKVWDLF YQADAVRAM LTRLIKEES LRTYLFTYS 
      HVYDSISMA TLAQMFELD KPVVHSIIS KMIINEELM 
      ASLDDPTQT VVMHRSEPS RLQSLALQL ADKINNFVD 
      SNERILETK QGNFFSRGM NQGNFRGDR QNYRGTNQD 
      WSRQRRDHR KEY
    CDS sequence
    • >TC015453 RA
      atgagtcgt tttttcgcc ggttcggac tcagactcc 
      gacagctcc tccgaggaa gagccaatt cagcgcgcg 
      ccagcgccc gttttcacc tttagcgac gatgaagag 
      gaggtcaaa cgtgtggtt cgctccgcc aaagagaag 
      cgttacgag gaactcact aacattatc aaacaaatc 
      cgcaattac aaaaaaatc aaagacatg agcagcatg 
      ttgaccagt tttgaagac cttcagaag gcctacgtt 
      aaagcgctc ccagtgatc gcgaaagaa gaatcgggg 
      caaacgccc cgtttcttc acccgttgt ttggtcgaa 
      atggaagat ttcatcaac gaaatgtgg gaagatcgc 
      gacggacgc aaaaacatg agcaaaaac aacagcaag 
      tctttggct tcaatgcgc cagaagttg cgcaagtac 
      atcaaagat ttcgaggag gatattgcc aagtttagg 
      gaaaacccc gaccagccg gacgatgaa gaagaggaa 
      gagaaacac gagcctgag ccagacact caaactgaa 
      gcgccggcc aaagctgtg gctaaaatt tccaaacag 
      attgttgat gatgaaggg tcgtctgat tcggacgat 
      tggcctagt gatagtgac tcgagttcg gatagtttt 
      gatgaagat gaagggaaa gggactatg agggagcgg 
      tttttgaag aaaactggg ccggcgcat gaagatgat 
      gaagaacgc gatcggagg aaggaggaa aggaggaag 
      gaacgtaag gagaaggag aggcggaag gtgaagaag 
      gaggatgag gaggatggg gaaggggag tgggagttt 
      gtgaagggg ggaatcgca attccgagc gagaagccg 
      aaaatgttc gccaaagac gcggaaatc gacctaaac 
      ctcgtaata aaaaaactc tccgaaata atggcagcc 
      cgtggcaaa aaacgcacc gaccgtcgc gaacaaatc 
      gaactcctc cacgaacta caaaacgtc tccgaccaa 
      caccaactc ggccccgcc atctacgtc aaaatcaaa 
      ttcgccata atttctgca attttcgac tacaacccg 
      aaagtctcc gacgcaatg aaacccgaa tactggatg 
      aaactactc gagcgcatg tccgaaatg ctcgacctt 
      ctcctcagc aacccccaa ctcttcgtc tcggaatcc 
      gtgaccgaa gaaacggaa aacctggaa gaaccccct 
      tacaaaatc cgcggctgt gtcctaact tccgtcgag 
      cgcctcgac gacgaattc acaaagcta ctaaaagag 
      tgcgacccg cacagcaac gaatacgtc gagcgcttg 
      aaagacgag gccaaagtc tcggaaatc atcgacaag 
      acgatgaaa tacctcgag cggacgaac gccccgtcc 
      gaactttgc cgcatttat ttgcgcaaa atcgagcat 
      ctgtattac aagttcgac ccgcgggtt ttacagcaa 
      aaagccggg gaaatcccc agggatttg tacacttcg 
      gtgcaagaa atggaaaag ttgtgcaaa ttcatttac 
      gcgaaagat ggcaccgat cggttgcgc acacgtgcg 
      attctctcc catatctac caccacgcc ctgcacgat 
      aactggttc caagcgcgt gacttggtc ctcatgtcc 
      cacctccag gagaccatc caccattcg gaccccagc 
      acccagatt ctctacaac cgtaccatg gcccatctg 
      ggtttgtgt gccttccgg cacgcgaat atcaaagac 
      gcgcataac tgtctcgtg gatttgatg atgaccggg 
      aagcccaag gagttgcta gcccagggg ttgctcccc 
      cagagacag catgagcgg agcaaagag caggagaaa 
      atcgaaaag cagcgccag atgcccttc catatgcac 
      atcaacttg gagcttttg gagtgcgtt tatttggtc 
      tcggcgatg ctgattgag attccctac atggcggcg 
      cacgagttc gacgcccgc cggcgcatg atctcgaag 
      actttttac cagcagctg cggagctcg gagaggcag 
      agcctggtg ggccccccg gagagcatg cgcgagcac 
      gtggtggcg gcggccaag gccatgcgc aacgggaac 
      tggtcggcg tgcaacggc ttcattatc aacgagaag 
      atgaacgcg aaagtttgg gatttgttt tatcaggcg 
      gacgcggtc agggcgatg ctgacgcgg ttgattaag 
      gaggagtcc ttgcggacg tacctcttc acttattcg 
      cacgtttac gactcgatt tccatggca acgctcgcg 
      caaatgttc gagttggat aagccggtg gttcactcg 
      attatttcc aaaatgatt atcaatgag gagctgatg 
      gcgtcgctc gatgacccg actcagacg gttgtcatg 
      catcgaagt gagccgtcg cgtttgcaa tcactggcg 
      ttgcagttg gcggataag attaataat ttcgttgat 
      tcgaatgag aggattttg gagacgaag caagggaat 
      ttcttttca agggggatg aatcaagga aatttcaga 
      ggagatagg cagaattat agggggacc aatcaggat 
      tggagtcgg caaaggaga gatcacagg aaagagtat 
      taa
    mRNA sequence
    • >TC015453 RA
      tcgtgccca aaaacaaat taatttgcc cttttttta 
      cgatattac cataacact accaatcac aagacaaaa 
      aagtcccga ttttgacag ttggcacaa gtttttggc 
      cattttgtg gttacaatc gtgtcggaa aatctgtta 
      agtgaaaaa aaggcttta aacacgtaa aaaaatgag 
      tcgtttttt cgccggttc ggactcaga ctccgacag 
      ctcctccga ggaagagcc aattcagcg cgcgccagc 
      gcccgtttt cacctttag cgacgatga agaggaggt 
      caaacgtgt ggttcgctc cgccaaaga gaagcgtta 
      cgaggaact cactaacat tatcaaaca aatccgcaa 
      ttacaaaaa aatcaaaga catgagcag catgttgac 
      cagttttga agaccttca gaaggccta cgttaaagc 
      gctcccagt gatcgcgaa agaagaatc ggggcaaac 
      gccccgttt cttcacccg ttgtttggt cgaaatgga 
      agatttcat caacgaaat gtgggaaga tcgcgacgg 
      acgcaaaaa catgagcaa aaacaacag caagtcttt 
      ggcttcaat gcgccagaa gttgcgcaa gtacatcaa 
      agatttcga ggaggatat tgccaagtt tagggaaaa 
      ccccgacca gccggacga tgaagaaga ggaagagaa 
      acacgagcc tgagccaga cactcaaac tgaagcgcc 
      ggccaaagc tgtggctaa aatttccaa acagattgt 
      tgatgatga agggtcgtc tgattcgga cgattggcc 
      tagtgatag tgactcgag ttcggatag ttttgatga 
      agatgaagg gaaagggac tatgaggga gcggttttt 
      gaagaaaac tgggccggc gcatgaaga tgatgaaga 
      acgcgatcg gaggaagga ggaaaggag gaaggaacg 
      taaggagaa ggagaggcg gaaggtgaa gaaggagga 
      tgaggagga tggggaagg ggagtggga gtttgtgaa 
      ggggggaat cgcaattcc gagcgagaa gccgaaaat 
      gttcgccaa agacgcgga aatcgacct aaacctcgt 
      aataaaaaa actctccga aataatggc agcccgtgg 
      caaaaaacg caccgaccg tcgcgaaca aatcgaact 
      cctccacga actacaaaa cgtctccga ccaacacca 
      actcggccc cgccatcta cgtcaaaat caaattcgc 
      cataatttc tgcaatttt cgactacaa cccgaaagt 
      ctccgacgc aatgaaacc cgaatactg gatgaaact 
      actcgagcg catgtccga aatgctcga ccttctcct 
      cagcaaccc ccaactctt cgtctcgga atccgtgac 
      cgaagaaac ggaaaacct ggaagaacc cccttacaa 
      aatccgcgg ctgtgtcct aacttccgt cgagcgcct 
      cgacgacga attcacaaa gctactaaa agagtgcga 
      cccgcacag caacgaata cgtcgagcg cttgaaaga 
      cgaggccaa agtctcgga aatcatcga caagacgat 
      gaaatacct cgagcggac gaacgcccc gtccgaact 
      ttgccgcat ttatttgcg caaaatcga gcatctgta 
      ttacaagtt cgacccgcg ggttttaca gcaaaaagc 
      cggggaaat ccccaggga tttgtacac ttcggtgca 
      agaaatgga aaagttgtg caaattcat ttacgcgaa 
      agatggcac cgatcggtt gcgcacacg tgcgattct 
      ctcccatat ctaccacca cgccctgca cgataactg 
      gttccaagc gcgtgactt ggtcctcat gtcccacct 
      ccaggagac catccacca ttcggaccc cagcaccca 
      gattctcta caaccgtac catggccca tctgggttt 
      gtgtgcctt ccggcacgc gaatatcaa agacgcgca 
      taactgtct cgtggattt gatgatgac cgggaagcc 
      caaggagtt gctagccca ggggttgct cccccagag 
      acagcatga gcggagcaa agagcagga gaaaatcga 
      aaagcagcg ccagatgcc cttccatat gcacatcaa 
      cttggagct tttggagtg cgtttattt ggtctcggc 
      gatgctgat tgagattcc ctacatggc ggcgcacga 
      gttcgacgc ccgccggcg catgatctc gaagacttt 
      ttaccagca gctgcggag ctcggagag gcagagcct 
      ggtgggccc cccggagag catgcgcga gcacgtggt 
      ggcggcggc caaggccat gcgcaacgg gaactggtc 
      ggcgtgcaa cggcttcat tatcaacga gaagatgaa 
      cgcgaaagt ttgggattt gttttatca ggcggacgc 
      ggtcagggc gatgctgac gcggttgat taaggagga 
      gtccttgcg gacgtacct cttcactta ttcgcacgt 
      ttacgactc gatttccat ggcaacgct cgcgcaaat 
      gttcgagtt ggataagcc ggtggttca ctcgattat 
      ttccaaaat gattatcaa tgaggagct gatggcgtc 
      gctcgatga cccgactca gacggttgt catgcatcg 
      aagtgagcc gtcgcgttt gcaatcact ggcgttgca 
      gttggcgga taagattaa taatttcgt tgattcgaa 
      tgagaggat tttggagac gaagcaagg gaatttctt 
      ttcaagggg gatgaatca aggaaattt cagaggaga 
      taggcagaa ttatagggg gaccaatca ggattggag 
      tcggcaaag gagagatca caggaaaga gtattaaga 
      gtgacagtc aggttgggt cttttttat ttgaaacca 
      ataaaaaac atacgagta tttttgtgc cctaactta 
      ccttttcta tggagatct cctcaattt aatttcaag 
      aattttact aaaatttat acaaaattg tcttcatca 
      atttgcaaa tccgggatt aggtgagac ggttgaagc 
      gtttttgaa ctaaaattg tttctttgt g
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY