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Gene information
General information
TC015453 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 9657551..9663313
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC015453
    iBeetle screen iB_02480

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 6 (9 dpi)
    Days post injection

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection

    larva lethality late (L7) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015453 RA
      MSRFFAGSD SDSDSSSEE EPIQRAPAP VFTFSDDEE 
EVKRVVRSA KEKRYEELT NIIKQIRNY KKIKDMSSM 
LTSFEDLQK AYVKALPVI AKEESGQTP RFFTRCLVE 
MEDFINEMW EDRDGRKNM SKNNSKSLA SMRQKLRKY 
IKDFEEDIA KFRENPDQP DDEEEEEKH EPEPDTQTE 
APAKAVAKI SKQIVDDEG SSDSDDWPS DSDSSSDSF 
DEDEGKGTM RERFLKKTG PAHEDDEER DRRKEERRK 
ERKEKERRK VKKEDEEDG EGEWEFVKG GIAIPSEKP 
KMFAKDAEI DLNLVIKKL SEIMAARGK KRTDRREQI 
ELLHELQNV SDQHQLGPA IYVKIKFAI ISAIFDYNP 
KVSDAMKPE YWMKLLERM SEMLDLLLS NPQLFVSES 
VTEETENLE EPPYKIRGC VLTSVERLD DEFTKLLKE 
CDPHSNEYV ERLKDEAKV SEIIDKTMK YLERTNAPS 
ELCRIYLRK IEHLYYKFD PRVLQQKAG EIPRDLYTS 
VQEMEKLCK FIYAKDGTD RLRTRAILS HIYHHALHD 
NWFQARDLV LMSHLQETI HHSDPSTQI LYNRTMAHL 
GLCAFRHAN IKDAHNCLV DLMMTGKPK ELLAQGLLP 
QRQHERSKE QEKIEKQRQ MPFHMHINL ELLECVYLV 
SAMLIEIPY MAAHEFDAR RRMISKTFY QQLRSSERQ 
SLVGPPESM REHVVAAAK AMRNGNWSA CNGFIINEK 
MNAKVWDLF YQADAVRAM LTRLIKEES LRTYLFTYS 
HVYDSISMA TLAQMFELD KPVVHSIIS KMIINEELM 
ASLDDPTQT VVMHRSEPS RLQSLALQL ADKINNFVD 
SNERILETK QGNFFSRGM NQGNFRGDR QNYRGTNQD 
WSRQRRDHR KEY
    CDS sequence
    • >TC015453 RA
      atgagtcgt tttttcgcc ggttcggac tcagactcc 
gacagctcc tccgaggaa gagccaatt cagcgcgcg 
ccagcgccc gttttcacc tttagcgac gatgaagag 
gaggtcaaa cgtgtggtt cgctccgcc aaagagaag 
cgttacgag gaactcact aacattatc aaacaaatc 
cgcaattac aaaaaaatc aaagacatg agcagcatg 
ttgaccagt tttgaagac cttcagaag gcctacgtt 
aaagcgctc ccagtgatc gcgaaagaa gaatcgggg 
caaacgccc cgtttcttc acccgttgt ttggtcgaa 
atggaagat ttcatcaac gaaatgtgg gaagatcgc 
gacggacgc aaaaacatg agcaaaaac aacagcaag 
tctttggct tcaatgcgc cagaagttg cgcaagtac 
atcaaagat ttcgaggag gatattgcc aagtttagg 
gaaaacccc gaccagccg gacgatgaa gaagaggaa 
gagaaacac gagcctgag ccagacact caaactgaa 
gcgccggcc aaagctgtg gctaaaatt tccaaacag 
attgttgat gatgaaggg tcgtctgat tcggacgat 
tggcctagt gatagtgac tcgagttcg gatagtttt 
gatgaagat gaagggaaa gggactatg agggagcgg 
tttttgaag aaaactggg ccggcgcat gaagatgat 
gaagaacgc gatcggagg aaggaggaa aggaggaag 
gaacgtaag gagaaggag aggcggaag gtgaagaag 
gaggatgag gaggatggg gaaggggag tgggagttt 
gtgaagggg ggaatcgca attccgagc gagaagccg 
aaaatgttc gccaaagac gcggaaatc gacctaaac 
ctcgtaata aaaaaactc tccgaaata atggcagcc 
cgtggcaaa aaacgcacc gaccgtcgc gaacaaatc 
gaactcctc cacgaacta caaaacgtc tccgaccaa 
caccaactc ggccccgcc atctacgtc aaaatcaaa 
ttcgccata atttctgca attttcgac tacaacccg 
aaagtctcc gacgcaatg aaacccgaa tactggatg 
aaactactc gagcgcatg tccgaaatg ctcgacctt 
ctcctcagc aacccccaa ctcttcgtc tcggaatcc 
gtgaccgaa gaaacggaa aacctggaa gaaccccct 
tacaaaatc cgcggctgt gtcctaact tccgtcgag 
cgcctcgac gacgaattc acaaagcta ctaaaagag 
tgcgacccg cacagcaac gaatacgtc gagcgcttg 
aaagacgag gccaaagtc tcggaaatc atcgacaag 
acgatgaaa tacctcgag cggacgaac gccccgtcc 
gaactttgc cgcatttat ttgcgcaaa atcgagcat 
ctgtattac aagttcgac ccgcgggtt ttacagcaa 
aaagccggg gaaatcccc agggatttg tacacttcg 
gtgcaagaa atggaaaag ttgtgcaaa ttcatttac 
gcgaaagat ggcaccgat cggttgcgc acacgtgcg 
attctctcc catatctac caccacgcc ctgcacgat 
aactggttc caagcgcgt gacttggtc ctcatgtcc 
cacctccag gagaccatc caccattcg gaccccagc 
acccagatt ctctacaac cgtaccatg gcccatctg 
ggtttgtgt gccttccgg cacgcgaat atcaaagac 
gcgcataac tgtctcgtg gatttgatg atgaccggg 
aagcccaag gagttgcta gcccagggg ttgctcccc 
cagagacag catgagcgg agcaaagag caggagaaa 
atcgaaaag cagcgccag atgcccttc catatgcac 
atcaacttg gagcttttg gagtgcgtt tatttggtc 
tcggcgatg ctgattgag attccctac atggcggcg 
cacgagttc gacgcccgc cggcgcatg atctcgaag 
actttttac cagcagctg cggagctcg gagaggcag 
agcctggtg ggccccccg gagagcatg cgcgagcac 
gtggtggcg gcggccaag gccatgcgc aacgggaac 
tggtcggcg tgcaacggc ttcattatc aacgagaag 
atgaacgcg aaagtttgg gatttgttt tatcaggcg 
gacgcggtc agggcgatg ctgacgcgg ttgattaag 
gaggagtcc ttgcggacg tacctcttc acttattcg 
cacgtttac gactcgatt tccatggca acgctcgcg 
caaatgttc gagttggat aagccggtg gttcactcg 
attatttcc aaaatgatt atcaatgag gagctgatg 
gcgtcgctc gatgacccg actcagacg gttgtcatg 
catcgaagt gagccgtcg cgtttgcaa tcactggcg 
ttgcagttg gcggataag attaataat ttcgttgat 
tcgaatgag aggattttg gagacgaag caagggaat 
ttcttttca agggggatg aatcaagga aatttcaga 
ggagatagg cagaattat agggggacc aatcaggat 
tggagtcgg caaaggaga gatcacagg aaagagtat 
taa
    mRNA sequence
    • >TC015453 RA
      tcgtgccca aaaacaaat taatttgcc cttttttta 
cgatattac cataacact accaatcac aagacaaaa 
aagtcccga ttttgacag ttggcacaa gtttttggc 
cattttgtg gttacaatc gtgtcggaa aatctgtta 
agtgaaaaa aaggcttta aacacgtaa aaaaatgag 
tcgtttttt cgccggttc ggactcaga ctccgacag 
ctcctccga ggaagagcc aattcagcg cgcgccagc 
gcccgtttt cacctttag cgacgatga agaggaggt 
caaacgtgt ggttcgctc cgccaaaga gaagcgtta 
cgaggaact cactaacat tatcaaaca aatccgcaa 
ttacaaaaa aatcaaaga catgagcag catgttgac 
cagttttga agaccttca gaaggccta cgttaaagc 
gctcccagt gatcgcgaa agaagaatc ggggcaaac 
gccccgttt cttcacccg ttgtttggt cgaaatgga 
agatttcat caacgaaat gtgggaaga tcgcgacgg 
acgcaaaaa catgagcaa aaacaacag caagtcttt 
ggcttcaat gcgccagaa gttgcgcaa gtacatcaa 
agatttcga ggaggatat tgccaagtt tagggaaaa 
ccccgacca gccggacga tgaagaaga ggaagagaa 
acacgagcc tgagccaga cactcaaac tgaagcgcc 
ggccaaagc tgtggctaa aatttccaa acagattgt 
tgatgatga agggtcgtc tgattcgga cgattggcc 
tagtgatag tgactcgag ttcggatag ttttgatga 
agatgaagg gaaagggac tatgaggga gcggttttt 
gaagaaaac tgggccggc gcatgaaga tgatgaaga 
acgcgatcg gaggaagga ggaaaggag gaaggaacg 
taaggagaa ggagaggcg gaaggtgaa gaaggagga 
tgaggagga tggggaagg ggagtggga gtttgtgaa 
ggggggaat cgcaattcc gagcgagaa gccgaaaat 
gttcgccaa agacgcgga aatcgacct aaacctcgt 
aataaaaaa actctccga aataatggc agcccgtgg 
caaaaaacg caccgaccg tcgcgaaca aatcgaact 
cctccacga actacaaaa cgtctccga ccaacacca 
actcggccc cgccatcta cgtcaaaat caaattcgc 
cataatttc tgcaatttt cgactacaa cccgaaagt 
ctccgacgc aatgaaacc cgaatactg gatgaaact 
actcgagcg catgtccga aatgctcga ccttctcct 
cagcaaccc ccaactctt cgtctcgga atccgtgac 
cgaagaaac ggaaaacct ggaagaacc cccttacaa 
aatccgcgg ctgtgtcct aacttccgt cgagcgcct 
cgacgacga attcacaaa gctactaaa agagtgcga 
cccgcacag caacgaata cgtcgagcg cttgaaaga 
cgaggccaa agtctcgga aatcatcga caagacgat 
gaaatacct cgagcggac gaacgcccc gtccgaact 
ttgccgcat ttatttgcg caaaatcga gcatctgta 
ttacaagtt cgacccgcg ggttttaca gcaaaaagc 
cggggaaat ccccaggga tttgtacac ttcggtgca 
agaaatgga aaagttgtg caaattcat ttacgcgaa 
agatggcac cgatcggtt gcgcacacg tgcgattct 
ctcccatat ctaccacca cgccctgca cgataactg 
gttccaagc gcgtgactt ggtcctcat gtcccacct 
ccaggagac catccacca ttcggaccc cagcaccca 
gattctcta caaccgtac catggccca tctgggttt 
gtgtgcctt ccggcacgc gaatatcaa agacgcgca 
taactgtct cgtggattt gatgatgac cgggaagcc 
caaggagtt gctagccca ggggttgct cccccagag 
acagcatga gcggagcaa agagcagga gaaaatcga 
aaagcagcg ccagatgcc cttccatat gcacatcaa 
cttggagct tttggagtg cgtttattt ggtctcggc 
gatgctgat tgagattcc ctacatggc ggcgcacga 
gttcgacgc ccgccggcg catgatctc gaagacttt 
ttaccagca gctgcggag ctcggagag gcagagcct 
ggtgggccc cccggagag catgcgcga gcacgtggt 
ggcggcggc caaggccat gcgcaacgg gaactggtc 
ggcgtgcaa cggcttcat tatcaacga gaagatgaa 
cgcgaaagt ttgggattt gttttatca ggcggacgc 
ggtcagggc gatgctgac gcggttgat taaggagga 
gtccttgcg gacgtacct cttcactta ttcgcacgt 
ttacgactc gatttccat ggcaacgct cgcgcaaat 
gttcgagtt ggataagcc ggtggttca ctcgattat 
ttccaaaat gattatcaa tgaggagct gatggcgtc 
gctcgatga cccgactca gacggttgt catgcatcg 
aagtgagcc gtcgcgttt gcaatcact ggcgttgca 
gttggcgga taagattaa taatttcgt tgattcgaa 
tgagaggat tttggagac gaagcaagg gaatttctt 
ttcaagggg gatgaatca aggaaattt cagaggaga 
taggcagaa ttatagggg gaccaatca ggattggag 
tcggcaaag gagagatca caggaaaga gtattaaga 
gtgacagtc aggttgggt cttttttat ttgaaacca 
ataaaaaac atacgagta tttttgtgc cctaactta 
ccttttcta tggagatct cctcaattt aatttcaag 
aattttact aaaatttat acaaaattg tcttcatca 
atttgcaaa tccgggatt aggtgagac ggttgaagc 
gtttttgaa ctaaaattg tttctttgt g
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY