| General information | |||
| TC015453 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 9657551..9663313 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC015453 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02480 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 6 (9 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
larva lethality late (L7) - Number of animals showing the phenotype: 4 (11 dpi)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
- >TC015453 RA
MSRFFAGSD SDSDSSSEE EPIQRAPAP VFTFSDDEE EVKRVVRSA KEKRYEELT NIIKQIRNY KKIKDMSSM LTSFEDLQK AYVKALPVI AKEESGQTP RFFTRCLVE MEDFINEMW EDRDGRKNM SKNNSKSLA SMRQKLRKY IKDFEEDIA KFRENPDQP DDEEEEEKH EPEPDTQTE APAKAVAKI SKQIVDDEG SSDSDDWPS DSDSSSDSF DEDEGKGTM RERFLKKTG PAHEDDEER DRRKEERRK ERKEKERRK VKKEDEEDG EGEWEFVKG GIAIPSEKP KMFAKDAEI DLNLVIKKL SEIMAARGK KRTDRREQI ELLHELQNV SDQHQLGPA IYVKIKFAI ISAIFDYNP KVSDAMKPE YWMKLLERM SEMLDLLLS NPQLFVSES VTEETENLE EPPYKIRGC VLTSVERLD DEFTKLLKE CDPHSNEYV ERLKDEAKV SEIIDKTMK YLERTNAPS ELCRIYLRK IEHLYYKFD PRVLQQKAG EIPRDLYTS VQEMEKLCK FIYAKDGTD RLRTRAILS HIYHHALHD NWFQARDLV LMSHLQETI HHSDPSTQI LYNRTMAHL GLCAFRHAN IKDAHNCLV DLMMTGKPK ELLAQGLLP QRQHERSKE QEKIEKQRQ MPFHMHINL ELLECVYLV SAMLIEIPY MAAHEFDAR RRMISKTFY QQLRSSERQ SLVGPPESM REHVVAAAK AMRNGNWSA CNGFIINEK MNAKVWDLF YQADAVRAM LTRLIKEES LRTYLFTYS HVYDSISMA TLAQMFELD KPVVHSIIS KMIINEELM ASLDDPTQT VVMHRSEPS RLQSLALQL ADKINNFVD SNERILETK QGNFFSRGM NQGNFRGDR QNYRGTNQD WSRQRRDHR KEY
- >TC015453 RA
atgagtcgt tttttcgcc ggttcggac tcagactcc gacagctcc tccgaggaa gagccaatt cagcgcgcg ccagcgccc gttttcacc tttagcgac gatgaagag gaggtcaaa cgtgtggtt cgctccgcc aaagagaag cgttacgag gaactcact aacattatc aaacaaatc cgcaattac aaaaaaatc aaagacatg agcagcatg ttgaccagt tttgaagac cttcagaag gcctacgtt aaagcgctc ccagtgatc gcgaaagaa gaatcgggg caaacgccc cgtttcttc acccgttgt ttggtcgaa atggaagat ttcatcaac gaaatgtgg gaagatcgc gacggacgc aaaaacatg agcaaaaac aacagcaag tctttggct tcaatgcgc cagaagttg cgcaagtac atcaaagat ttcgaggag gatattgcc aagtttagg gaaaacccc gaccagccg gacgatgaa gaagaggaa gagaaacac gagcctgag ccagacact caaactgaa gcgccggcc aaagctgtg gctaaaatt tccaaacag attgttgat gatgaaggg tcgtctgat tcggacgat tggcctagt gatagtgac tcgagttcg gatagtttt gatgaagat gaagggaaa gggactatg agggagcgg tttttgaag aaaactggg ccggcgcat gaagatgat gaagaacgc gatcggagg aaggaggaa aggaggaag gaacgtaag gagaaggag aggcggaag gtgaagaag gaggatgag gaggatggg gaaggggag tgggagttt gtgaagggg ggaatcgca attccgagc gagaagccg aaaatgttc gccaaagac gcggaaatc gacctaaac ctcgtaata aaaaaactc tccgaaata atggcagcc cgtggcaaa aaacgcacc gaccgtcgc gaacaaatc gaactcctc cacgaacta caaaacgtc tccgaccaa caccaactc ggccccgcc atctacgtc aaaatcaaa ttcgccata atttctgca attttcgac tacaacccg aaagtctcc gacgcaatg aaacccgaa tactggatg aaactactc gagcgcatg tccgaaatg ctcgacctt ctcctcagc aacccccaa ctcttcgtc tcggaatcc gtgaccgaa gaaacggaa aacctggaa gaaccccct tacaaaatc cgcggctgt gtcctaact tccgtcgag cgcctcgac gacgaattc acaaagcta ctaaaagag tgcgacccg cacagcaac gaatacgtc gagcgcttg aaagacgag gccaaagtc tcggaaatc atcgacaag acgatgaaa tacctcgag cggacgaac gccccgtcc gaactttgc cgcatttat ttgcgcaaa atcgagcat ctgtattac aagttcgac ccgcgggtt ttacagcaa aaagccggg gaaatcccc agggatttg tacacttcg gtgcaagaa atggaaaag ttgtgcaaa ttcatttac gcgaaagat ggcaccgat cggttgcgc acacgtgcg attctctcc catatctac caccacgcc ctgcacgat aactggttc caagcgcgt gacttggtc ctcatgtcc cacctccag gagaccatc caccattcg gaccccagc acccagatt ctctacaac cgtaccatg gcccatctg ggtttgtgt gccttccgg cacgcgaat atcaaagac gcgcataac tgtctcgtg gatttgatg atgaccggg aagcccaag gagttgcta gcccagggg ttgctcccc cagagacag catgagcgg agcaaagag caggagaaa atcgaaaag cagcgccag atgcccttc catatgcac atcaacttg gagcttttg gagtgcgtt tatttggtc tcggcgatg ctgattgag attccctac atggcggcg cacgagttc gacgcccgc cggcgcatg atctcgaag actttttac cagcagctg cggagctcg gagaggcag agcctggtg ggccccccg gagagcatg cgcgagcac gtggtggcg gcggccaag gccatgcgc aacgggaac tggtcggcg tgcaacggc ttcattatc aacgagaag atgaacgcg aaagtttgg gatttgttt tatcaggcg gacgcggtc agggcgatg ctgacgcgg ttgattaag gaggagtcc ttgcggacg tacctcttc acttattcg cacgtttac gactcgatt tccatggca acgctcgcg caaatgttc gagttggat aagccggtg gttcactcg attatttcc aaaatgatt atcaatgag gagctgatg gcgtcgctc gatgacccg actcagacg gttgtcatg catcgaagt gagccgtcg cgtttgcaa tcactggcg ttgcagttg gcggataag attaataat ttcgttgat tcgaatgag aggattttg gagacgaag caagggaat ttcttttca agggggatg aatcaagga aatttcaga ggagatagg cagaattat agggggacc aatcaggat tggagtcgg caaaggaga gatcacagg aaagagtat taa
- >TC015453 RA
tcgtgccca aaaacaaat taatttgcc cttttttta cgatattac cataacact accaatcac aagacaaaa aagtcccga ttttgacag ttggcacaa gtttttggc cattttgtg gttacaatc gtgtcggaa aatctgtta agtgaaaaa aaggcttta aacacgtaa aaaaatgag tcgtttttt cgccggttc ggactcaga ctccgacag ctcctccga ggaagagcc aattcagcg cgcgccagc gcccgtttt cacctttag cgacgatga agaggaggt caaacgtgt ggttcgctc cgccaaaga gaagcgtta cgaggaact cactaacat tatcaaaca aatccgcaa ttacaaaaa aatcaaaga catgagcag catgttgac cagttttga agaccttca gaaggccta cgttaaagc gctcccagt gatcgcgaa agaagaatc ggggcaaac gccccgttt cttcacccg ttgtttggt cgaaatgga agatttcat caacgaaat gtgggaaga tcgcgacgg acgcaaaaa catgagcaa aaacaacag caagtcttt ggcttcaat gcgccagaa gttgcgcaa gtacatcaa agatttcga ggaggatat tgccaagtt tagggaaaa ccccgacca gccggacga tgaagaaga ggaagagaa acacgagcc tgagccaga cactcaaac tgaagcgcc ggccaaagc tgtggctaa aatttccaa acagattgt tgatgatga agggtcgtc tgattcgga cgattggcc tagtgatag tgactcgag ttcggatag ttttgatga agatgaagg gaaagggac tatgaggga gcggttttt gaagaaaac tgggccggc gcatgaaga tgatgaaga acgcgatcg gaggaagga ggaaaggag gaaggaacg taaggagaa ggagaggcg gaaggtgaa gaaggagga tgaggagga tggggaagg ggagtggga gtttgtgaa ggggggaat cgcaattcc gagcgagaa gccgaaaat gttcgccaa agacgcgga aatcgacct aaacctcgt aataaaaaa actctccga aataatggc agcccgtgg caaaaaacg caccgaccg tcgcgaaca aatcgaact cctccacga actacaaaa cgtctccga ccaacacca actcggccc cgccatcta cgtcaaaat caaattcgc cataatttc tgcaatttt cgactacaa cccgaaagt ctccgacgc aatgaaacc cgaatactg gatgaaact actcgagcg catgtccga aatgctcga ccttctcct cagcaaccc ccaactctt cgtctcgga atccgtgac cgaagaaac ggaaaacct ggaagaacc cccttacaa aatccgcgg ctgtgtcct aacttccgt cgagcgcct cgacgacga attcacaaa gctactaaa agagtgcga cccgcacag caacgaata cgtcgagcg cttgaaaga cgaggccaa agtctcgga aatcatcga caagacgat gaaatacct cgagcggac gaacgcccc gtccgaact ttgccgcat ttatttgcg caaaatcga gcatctgta ttacaagtt cgacccgcg ggttttaca gcaaaaagc cggggaaat ccccaggga tttgtacac ttcggtgca agaaatgga aaagttgtg caaattcat ttacgcgaa agatggcac cgatcggtt gcgcacacg tgcgattct ctcccatat ctaccacca cgccctgca cgataactg gttccaagc gcgtgactt ggtcctcat gtcccacct ccaggagac catccacca ttcggaccc cagcaccca gattctcta caaccgtac catggccca tctgggttt gtgtgcctt ccggcacgc gaatatcaa agacgcgca taactgtct cgtggattt gatgatgac cgggaagcc caaggagtt gctagccca ggggttgct cccccagag acagcatga gcggagcaa agagcagga gaaaatcga aaagcagcg ccagatgcc cttccatat gcacatcaa cttggagct tttggagtg cgtttattt ggtctcggc gatgctgat tgagattcc ctacatggc ggcgcacga gttcgacgc ccgccggcg catgatctc gaagacttt ttaccagca gctgcggag ctcggagag gcagagcct ggtgggccc cccggagag catgcgcga gcacgtggt ggcggcggc caaggccat gcgcaacgg gaactggtc ggcgtgcaa cggcttcat tatcaacga gaagatgaa cgcgaaagt ttgggattt gttttatca ggcggacgc ggtcagggc gatgctgac gcggttgat taaggagga gtccttgcg gacgtacct cttcactta ttcgcacgt ttacgactc gatttccat ggcaacgct cgcgcaaat gttcgagtt ggataagcc ggtggttca ctcgattat ttccaaaat gattatcaa tgaggagct gatggcgtc gctcgatga cccgactca gacggttgt catgcatcg aagtgagcc gtcgcgttt gcaatcact ggcgttgca gttggcgga taagattaa taatttcgt tgattcgaa tgagaggat tttggagac gaagcaagg gaatttctt ttcaagggg gatgaatca aggaaattt cagaggaga taggcagaa ttatagggg gaccaatca ggattggag tcggcaaag gagagatca caggaaaga gtattaaga gtgacagtc aggttgggt cttttttat ttgaaacca ataaaaaac atacgagta tttttgtgc cctaactta ccttttcta tggagatct cctcaattt aatttcaag aattttact aaaatttat acaaaattg tcttcatca atttgcaaa tccgggatt aggtgagac ggttgaagc gtttttgaa ctaaaattg tttctttgt g
