| General information | |||
| TC034842 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 9702163..9714803 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC034842 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02479 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae -
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC034842 RA
MSEIRRSLR LQSKNHFVK TEPKARRKI LRSFGSFDV PFNNCKYKY RGCKERFPE DELKRHELE CHFRWYKCE GKIFAGWSC NWKGYHEDI MNHFKKEHR ERTYMKDQN VIEMSLILD QDSYDLQLI RKRKQLFWF KHRLLSEEK AAYWLCQYV GPQKEALTY CYELVVSSN EVRKFAVTE LCYTDALDA NKIYEAGRC VVMTFDQIK SFMNGEGKV TVYFRIKKN VKAKNIKYE QINAEIFTS NMTETITIK NFSSLLSLP PSKKLKECK IARLTAPGE NYGSLMLKV DVTLTTTSG GSETLHLVA KMVPPNPDI QEIFNTSVT FRNEIEFYR KIVPALRNF QKKFGPEVV NFFPKYFGS RLNLTGSDH VDPDAVLLL DNLKIANYV GLDRCAGLD ISVTRLILR NLATLHAVP IGLKLQQPE VFASEVRPF LSPWLPKRE MHDKYRAAI AKMAAEIPE IAHLTQRIL DGFDRELIP EVPREPFAT IAHNDCWAS NSLVKFESG EAVDCKLVD YQVCGYGSP ARDVIFFLF SSVGIEGLE QLEHLLKFY HDCFISTLK KVGCDVGRF GFEEFGKEL DFEARNSQF GHVAFMLFV VFTDKTLIK EIKEFSVDN IIAGAGSEE HKRKFAFVV EEFAKRNWI
- >TC034842 RA
atgtcagag ataaggcgt tcattacgt ttacaaagc aagaatcat ttcgtcaaa actgagcca aaggcaagg agaaagata ttacggtct tttggtagc tttgacgtc ccttttaac aactgtaaa tacaaatac cgcggttgc aaggaacgc ttccctgaa gatgagctc aaaaggcat gagctggaa tgccacttt cgttggtac aaatgcgag gggaaaata tttgcggga tggtcctgt aactggaaa ggctatcac gaggacatc atgaaccac ttcaagaag gagcaccgg gaaagaacc tacatgaaa gaccaaaac gttatagaa atgtcgctg atcctggac caggactcg tacgatttg caattgatt cgaaagcga aagcagctt ttctggttc aaacaccga cttttgagt gaggagaag gcggcgtat tggttgtgt caatacgta gggccgcag aaagaagca ctaacttac tgttacgag ctggttgtg agcagtaat gaagtacgg aaatttgcg gtaaccgaa ttgtgttac acggatgct ttagacgcg aataaaatt tacgaagcc ggaaggtgt gtcgttatg acgttcgat cagattaag agtttcatg aatggagag gggaaggta acagtttat ttcaggatc aagaagaat gttaaggcg aagaatata aaatatgaa caaatcaat gctgaaatt ttcacatca aacatgact gaaacaatc acgatcaaa aacttctcc tccttacta tcacttcca ccgtcaaaa aaactcaag gaatgtaaa atcgcgcgt ctcacagct ccgggtgaa aactacgga agtttaatg ctaaaggtg gacgtaacc ctgaccaca acttccggc ggaagtgaa accctccat cttgtcgcc aaaatggtg ccgccgaac cccgacatc caggaaatc ttcaacact tcggtcact ttccgcaac gaaatcgaa ttctaccga aaaatcgtg cccgcctta cgcaacttt caaaaaaaa tttggaccg gaagtcgtc aacttcttc ccgaaatat ttcggaagt cggcttaac ctcaccgga agtgaccac gtggacccc gacgccgtc ctcctattg gacaatttg aaaatcgca aactatgtc ggactggac cgatgtgct ggactcgac atttccgtg actcgccta attttgcga aacttggca acactgcac gccgtcccc attggctta aaactgcaa caaccggaa gtcttcgct tcggaagtg cgcccattt ctatcacca tggttacca aagcgcgaa atgcacgac aagtatcgc gccgccatt gccaaaatg gcggccgaa attcccgaa atcgcccac ttgacgcag cgaatcctt gacgggttc gaccgggaa cttatcccg gaagtcccg cgggaaccg ttcgccacc atagcccac aacgattgc tgggctagc aacagtttg gtgaaattc gagtcaggg gaggctgtg gattgcaaa ctcgttgat tatcaagtg tgcggttac ggatcgccg gctcgtgac gtcattttc ttccttttt tcaagtgta gggattgag ggtttggaa cagttggaa cacctcctt aagttttac cacgattgt ttcatctcc actctcaag aaagttggc tgtgatgtt gggcggttt ggttttgag gagtttggc aaggagttg gattttgag gcgaggaat tcgcagttt gggcacgtg gcttttatg cttttcgtt gttttcacc gataaaacc ttgattaag gagattaag gagttttcc gttgataat atcatagca ggcgctggg agcgaggag cataagagg aagtttgcg tttgttgtg gaggagttt gctaaaagg aattggatt tga
- >TC034842 RA
atagttcct cttctgaaa ctgaaatgt cagagataa ggcgttcat tacgtttac aaagcaaga atcatttcg tcaaaactg agccaaagg caaggagaa agatattac ggtcttttg gtagctttg acgtccctt ttaacaact gtaaataca aataccgcg gttgcaagg aacgcttcc ctgaagatg agctcaaaa ggcatgagc tggaatgcc actttcgtt ggtacaaat gcgagggga aaatatttg cgggatggt cctgtaact ggaaaggct atcacgagg acatcatga accacttca agaaggagc accgggaaa gaacctaca tgaaagacc aaaacgtta tagaaatgt cgctgatcc tggaccagg actcgtacg atttgcaat tgattcgaa agcgaaagc agcttttct ggttcaaac accgacttt tgagtgagg agaaggcgg cgtattggt tgtgtcaat acgtagggc cgcagaaag aagcactaa cttactgtt acgagctgg ttgtgagca gtaatgaag tacggaaat ttgcggtaa ccgaattgt gttacacgg atgctttag acgcgaata aaatttacg aagccggaa ggtgtgtcg ttatgacgt tcgatcaga ttaagagtt tcatgaatg gagagggga aggtaacag tttatttca ggatcaaga agaatgtta aggcgaaga atataaaat atgaacaaa tcaatgctg aaattttca catcaaaca tgactgaaa caatcacga tcaaaaact tctcctcct tactatcac ttccaccgt caaaaaaac tcaaggaat gtaaaatcg cgcgtctca cagctccgg gtgaaaact acggaagtt taatgctaa aggtggacg taaccctga ccacaactt ccggcggaa gtgaaaccc tccatcttg tcgccaaaa tggtgccgc cgaaccccg acatccagg aaatcttca acacttcgg tcactttcc gcaacgaaa tcgaattct accgaaaaa tcgtgcccg ccttacgca actttcaaa aaaaatttg gaccggaag tcgtcaact tcttcccga aatatttcg gaagtcggc ttaacctca ccggaagtg accacgtgg accccgacg ccgtcctcc tattggaca atttgaaaa tcgcaaact atgtcggac tggaccgat gtgctggac tcgacattt ccgtgactc gcctaattt tgcgaaact tggcaacac tgcacgccg tccccattg gcttaaaac tgcaacaac cggaagtct tcgcttcgg aagtgcgcc catttctat caccatggt taccaaagc gcgaaatgc acgacaagt atcgcgccg ccattgcca aaatggcgg ccgaaattc ccgaaatcg cccacttga cgcagcgaa tccttgacg ggttcgacc gggaactta tcccggaag tcccgcggg aaccgttcg ccaccatag cccacaacg attgctggg ctagcaaca gtttggtga aattcgagt caggggagg ctgtggatt gcaaactcg ttgattatc aagtgtgcg gttacggat cgccggctc gtgacgtca ttttcttcc ttttttcaa gtgtaggga ttgagggtt tggaacagt tggaacacc tccttaagt tttaccacg attgtttca tctccactc tcaagaaag ttggctgtg atgttgggc ggtttggtt ttgaggagt ttggcaagg agttggatt ttgaggcga ggaattcgc agtttgggc acgtggctt ttatgcttt tcgttgttt tcaccgata aaaccttga ttaaggaga ttaaggagt tttccgttg ataatatca tagcaggcg ctgggagcg aggagcata agaggaagt ttgcgtttg ttgtggagg agtttgcta aaaggaatt ggatttgag gggcagctg aagaaaaat tgtgtttgg actgtgata agcaaaaat ataaatact tgcactgta gataaggtt gcaaaatat tggtttgtg gcaataaaa tttgaaaaa atta
