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Gene information
General information
TC033326
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC015431
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid39: 784641..786200
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033326
    iBeetle screen iB_02477
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033326 RA
      MELYKCENC TFVTKLPFN TTNHLTIHA KGSETFDCS 
      SDLEPYKCH KCQTFETPL LSQMRHHNL QGHTSQSVK 
      LTINKCPSC DFTSFSRYL FARHLITSN HQKKEVYWC 
      KNCNFTTNF PYNLKRHVI LRHTDDSQI HWFKCKHCQ 
      FKAKNKDNL RKHLINRHE AKNVNPAQL LVCDHCDYK 
      CTKKYRLKE HKLAMHSLL CDFEYFPCD QCQFKTLFK 
      STLNKHVTV THGSDWFKC DQCKFETKY KQNLQNHLL 
      TKHNHNPKL FECDKCGHK TKTKATLIK HRKHKHENT 
      PLFHCDICD YTTNVNRNL IKHCDARHA TGYHKCNKC 
      DLSFETRFE LTTHTKNEH FKVIVKYQC HLCDFKTNS 
      KLELATHKK TEHLKNVKL YECQLCDFK SEHKGNFGN 
      HKLQKHSDK WLKCEQCGY QTKWKSSLL RHVRTQHG
    CDS sequence
    • >TC033326 RA
      atggagctg tacaagtgc gaaaattgc acttttgtg 
      accaaactc ccatttaat acaacaaac cacttaaca 
      attcatgca aaaggcagc gaaactttt gattgttcg 
      agtgacctt gagccctac aagtgtcac aaatgtcag 
      acatttgag acgccattg ttgtcacaa atgagacat 
      cacaacctt caaggtcac acatcacaa agtgtcaaa 
      cttacaatt aacaagtgt cccagttgt gatttcacg 
      tcgttttcg agatatttg tttgcgcga catttaatt 
      acgtccaat catcaaaaa aaggaggtt tactggtgc 
      aaaaattgc aatttcacg acgaatttt ccctacaat 
      ttgaaacgc cacgtgatt ttgagacat actgacgat 
      tctcaaatc cattggttt aagtgtaaa cattgtcaa 
      ttcaaggcc aaaaacaag gacaatttg cgcaaacac 
      ttgattaat cggcacgaa gccaaaaat gtaaatccc 
      gcccaactt ttggtttgt gatcattgt gattacaag 
      tgtacgaaa aaataccgc ttaaaagaa cacaaacta 
      gcaatgcat tcgttgttg tgcgacttt gagtacttc 
      ccctgcgac caatgccag ttcaagacg ctgtttaaa 
      agtacgcta aacaagcac gtgaccgta acacacggc 
      tccgattgg ttcaagtgc gaccaatgc aaattcgaa 
      acaaaatac aaacaaaac ttgcaaaac caccttctc 
      actaaacac aatcacaat cctaaattg tttgaatgt 
      gataaatgt ggccacaaa actaaaaca aaggccact 
      ctgatcaaa caccgaaag cataaacat gaaaacaca 
      ccattattc cactgtgac atttgtgat tacaccaca 
      aatgtcaac cgcaattta attaaacat tgtgatgcg 
      cgtcatgcc acaggttat cacaaatgt aacaagtgt 
      gatttgagc ttcgagacg cgatttgag ctcacaacg 
      cacacaaaa aacgaacat ttcaaggtt attgtcaaa 
      tatcaatgc cacctttgt gactttaaa actaattca 
      aagctggaa ttggccaca cataagaaa accgaacat 
      ttgaagaac gttaaattg tatgagtgc caactttgt 
      gattttaaa agtgagcat aaggggaac ttcggcaat 
      cacaaattg cagaaacat tcggataag tggcttaag 
      tgcgaacag tgcggttat caaacgaag tggaaaagc 
      agtttgctg aggcatgtg aggacacag catggttga
    mRNA sequence
    • >TC033326 RA
      attatttaa ttttttgtg tgatttacg gtgtgttgc 
      tcacatttg actgttaaa atgagaaaa atacaaaaa 
      acaatcttt attaatcct tcacaaagt cgtgtattt 
      gttaattga ttttcgtca tggagctgt acaagtgcg 
      aaaattgca cttttgtga ccaaactcc catttaata 
      caacaaacc acttaacaa ttcatgcaa aaggcagcg 
      aaacttttg attgttcga gtgaccttg agccctaca 
      agtgtcaca aatgtcaga catttgaga cgccattgt 
      tgtcacaaa tgagacatc acaaccttc aaggtcaca 
      catcacaaa gtgtcaaac ttacaatta acaagtgtc 
      ccagttgtg atttcacgt cgttttcga gatatttgt 
      ttgcgcgac atttaatta cgtccaatc atcaaaaaa 
      aggaggttt actggtgca aaaattgca atttcacga 
      cgaattttc cctacaatt tgaaacgcc acgtgattt 
      tgagacata ctgacgatt ctcaaatcc attggttta 
      agtgtaaac attgtcaat tcaaggcca aaaacaagg 
      acaatttgc gcaaacact tgattaatc ggcacgaag 
      ccaaaaatg taaatcccg cccaacttt tggtttgtg 
      atcattgtg attacaagt gtacgaaaa aataccgct 
      taaaagaac acaaactag caatgcatt cgttgttgt 
      gcgactttg agtacttcc cctgcgacc aatgccagt 
      tcaagacgc tgtttaaaa gtacgctaa acaagcacg 
      tgaccgtaa cacacggct ccgattggt tcaagtgcg 
      accaatgca aattcgaaa caaaataca aacaaaact 
      tgcaaaacc accttctca ctaaacaca atcacaatc 
      ctaaattgt ttgaatgtg ataaatgtg gccacaaaa 
      ctaaaacaa aggccactc tgatcaaac accgaaagc 
      ataaacatg aaaacacac cattattcc actgtgaca 
      tttgtgatt acaccacaa atgtcaacc gcaatttaa 
      ttaaacatt gtgatgcgc gtcatgcca caggttatc 
      acaaatgta acaagtgtg atttgagct tcgagacgc 
      gatttgagc tcacaacgc acacaaaaa acgaacatt 
      tcaaggtta ttgtcaaat atcaatgcc acctttgtg 
      actttaaaa ctaattcaa agctggaat tggccacac 
      ataagaaaa ccgaacatt tgaagaacg ttaaattgt 
      atgagtgcc aactttgtg attttaaaa gtgagcata 
      aggggaact tcggcaatc acaaattgc agaaacatt 
      cggataagt ggcttaagt gcgaacagt gcggttatc 
      aaacgaagt ggaaaagca gtttgctga ggcatgtga 
      ggacacagc atggttgat taaactgat tttgccgca 
      atttgtgtt ttatttcaa aatttgtgc actttcttt 
      caaagaatt taataatgt taccagcgt ttt