Status
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Gene information
General information
TC015430 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 780774..783948
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015430
    iBeetle screen iB_02476

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    head appendages random not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015430 RA
      MASAAELPS ADNSWIEFC ERHASVAAQ DFSKSCVQF 
      MSLNLSESA KATITHKDF LGKFLETFA EHFETDFGK 
      RKAQVAKIA NGGSRLDDD ASEPEEGSP KMPHKPFFR 
      RLSFKMLRK GKDLFHKQH SDEVDLAHN KTKLAKIVV 
      ECRKEGIVS YSTPESLDQ PGGPPKWEK CRLALVKAV 
      GGYMLEFYS PPKSTKPRS GVFCALITE ARETTALEM 
      PDHENTFVL KANNSLEFV ILARDTDDM RSWLATIKY 
      CIRSGAGYG DTAPGDQRD YHSDAPELP PRHVSGRGG 
      DRLSSSSNF DMCPSTGDN ISMETDIGQ TLKKEFWFH 
      GTLGRSEAT NLVLHDGAR GHGLFLVRQ SETRTGEFV 
      LTFNFQGRA KHLRMTIND QGQCRVQHF WFQSIYEML 
      EHFRQQPIP LESGGNSDV TLTDFVVNS AARGQLQGS 
      MGRSSQAST NGVHERRVP PIPEIRQVR
    • >TC015430 RB
      MASAAELPS ADNSWIEFC ERHASVAAQ DFSKSCVQF 
      MSLNLSESA KATITHKDF LGKFLETFA EHFETDFGK 
      RKAQVAKIA NGGSRLDDD ASEPEEGSP KMPHKPFFR 
      RLSFKMLRK GKDLFHKQH SDEVDLAHN KTKLAKIVV 
      ECRKEGIVS YSTPESLDQ PGGPPKWEK CRLALVKAV 
      GGYMLEFYS PPKSTKPRS GVFCALITE ARETTALEM 
      PDHENTFVL KANNSLEFV ILARDTDDM RSWLATIKY 
      CIRSGAGYG DTAPGDQRD YHSDAPELP PRHVSGRGG 
      DRLSSSSNF DMCPSTGDN ISMETDIGQ TLKKEFWFH 
      GTLGRSEAT NLVLHDGAR GHGLFLVRQ SETRTGEFV 
      LTFNFQGRA KHLRMTIND QGQCRVQHF WFQSIYEML 
      EHFRQQPIP LESGGNSDV TLTDFVVNS AARGQLQGS 
      MGRSSQAST NGVHERRVP PIPEIRQVQ THNGSVRTQ 
      ETTLEQISE ARAVENQYS FV
    CDS sequence
    • >TC015430 RA
      atggcgagt gcggcggag ctcccgtcg gccgacaac 
      tcgtggatc gaattctgc gagcggcat gccagtgtc 
      gccgctcaa gacttctcc aaaagttgc gtccagttc 
      atgtcgtta aacctttcg gaatcggcc aaagcgaca 
      ataacccac aaagacttc cttggcaaa ttcctcgag 
      acgttcgcc gagcacttc gagaccgac ttcggcaag 
      cgcaaggcc caggtggcg aaaatcgcg aatgggggc 
      tcccgcctc gacgacgac gcctcggag cccgaggag 
      ggctccccg aagatgccc cacaagccg ttcttccgc 
      aggttaagt ttcaaaatg ctgcggaag ggcaaagac 
      ctcttccac aagcagcac tcggacgaa gtggacttg 
      gcgcacaac aagaccaag ttggccaag atcgtggtc 
      gagtgccgc aaggagggg attgtgagc tactccacc 
      ccagagagc ttggaccag cccgggggg ccccccaag 
      tgggagaag tgccggctg gcgctggtc aaggccgtc 
      ggggggtac atgctcgag ttctactcg ccgcccaaa 
      agcaccaaa ccccgtagt ggcgttttt tgcgccctt 
      atcaccgaa gcccgcgag actacggct ctggaaatg 
      ccagatcat gaaaatact ttcgttctt aaggctaat 
      aatagtctc gaatttgtt attttggcc agagatacc 
      gatgatatg aggtcatgg cttgctaca attaagtat 
      tgtatacgt tcgggggcg ggttacggt gacaccgcc 
      cccggcgat caacgcgac tatcactcg gacgccccc 
      gaactgccc ccgagacat gtctcaggc cgagggggc 
      gaccggttg tcttccagt agtaacttc gatatgtgc 
      ccctcaacc ggcgataat atctcaatg gagactgat 
      attggccag acgttaaaa aaagaattc tggttccat 
      ggcactcta ggccggtcc gaagccacc aatctagtc 
      ctccatgac ggcgctaga ggccatgga ttgtttctg 
      gtacgtcag agtgagaca cggaccggc gaattcgtc 
      ctaactttc aattttcaa ggccgggct aagcacctc 
      cggatgacc atcaacgac caaggccag tgccgagtg 
      caacacttc tggttccag tccatctac gaaatgctc 
      gaacacttc cgacagcag ccgattccg ctcgagagc 
      ggcggcaat tccgacgtc acactcacc gacttcgtt 
      gtcaattcc gccgcaaga gggcagctc caaggctcg 
      atggggcgg agctcgcaa gcctcgacc aatggcgtg 
      cacgaacgc agagtcccg cccataccc gaaatcaga 
      caagtgaga taa
    • >TC015430 RB
      atggcgagt gcggcggag ctcccgtcg gccgacaac 
      tcgtggatc gaattctgc gagcggcat gccagtgtc 
      gccgctcaa gacttctcc aaaagttgc gtccagttc 
      atgtcgtta aacctttcg gaatcggcc aaagcgaca 
      ataacccac aaagacttc cttggcaaa ttcctcgag 
      acgttcgcc gagcacttc gagaccgac ttcggcaag 
      cgcaaggcc caggtggcg aaaatcgcg aatgggggc 
      tcccgcctc gacgacgac gcctcggag cccgaggag 
      ggctccccg aagatgccc cacaagccg ttcttccgc 
      aggttaagt ttcaaaatg ctgcggaag ggcaaagac 
      ctcttccac aagcagcac tcggacgaa gtggacttg 
      gcgcacaac aagaccaag ttggccaag atcgtggtc 
      gagtgccgc aaggagggg attgtgagc tactccacc 
      ccagagagc ttggaccag cccgggggg ccccccaag 
      tgggagaag tgccggctg gcgctggtc aaggccgtc 
      ggggggtac atgctcgag ttctactcg ccgcccaaa 
      agcaccaaa ccccgtagt ggcgttttt tgcgccctt 
      atcaccgaa gcccgcgag actacggct ctggaaatg 
      ccagatcat gaaaatact ttcgttctt aaggctaat 
      aatagtctc gaatttgtt attttggcc agagatacc 
      gatgatatg aggtcatgg cttgctaca attaagtat 
      tgtatacgt tcgggggcg ggttacggt gacaccgcc 
      cccggcgat caacgcgac tatcactcg gacgccccc 
      gaactgccc ccgagacat gtctcaggc cgagggggc 
      gaccggttg tcttccagt agtaacttc gatatgtgc 
      ccctcaacc ggcgataat atctcaatg gagactgat 
      attggccag acgttaaaa aaagaattc tggttccat 
      ggcactcta ggccggtcc gaagccacc aatctagtc 
      ctccatgac ggcgctaga ggccatgga ttgtttctg 
      gtacgtcag agtgagaca cggaccggc gaattcgtc 
      ctaactttc aattttcaa ggccgggct aagcacctc 
      cggatgacc atcaacgac caaggccag tgccgagtg 
      caacacttc tggttccag tccatctac gaaatgctc 
      gaacacttc cgacagcag ccgattccg ctcgagagc 
      ggcggcaat tccgacgtc acactcacc gacttcgtt 
      gtcaattcc gccgcaaga gggcagctc caaggctcg 
      atggggcgg agctcgcaa gcctcgacc aatggcgtg 
      cacgaacgc agagtcccg cccataccc gaaatcaga 
      caagtccaa acacacaat gggagtgtg cgaactcag 
      gaaaccact ctggaacaa atttccgag gcacgagct 
      gttgaaaat cagtacagt tttgtgtaa
    mRNA sequence
    • >TC015430 RA
      tgtgaaagc tgccgctta acgtcaaaa caataataa 
      atcaaccgc aaactatgt gattctgag caagtagtg 
      cccccttga agagtccaa tggcgagtg cggcggagc 
      tcccgtcgg ccgacaact cgtggatcg aattctgcg 
      agcggcatg ccagtgtcg ccgctcaag acttctcca 
      aaagttgcg tccagttca tgtcgttaa acctttcgg 
      aatcggcca aagcgacaa taacccaca aagacttcc 
      ttggcaaat tcctcgaga cgttcgccg agcacttcg 
      agaccgact tcggcaagc gcaaggccc aggtggcga 
      aaatcgcga atgggggct cccgcctcg acgacgacg 
      cctcggagc ccgaggagg gctccccga agatgcccc 
      acaagccgt tcttccgca ggttaagtt tcaaaatgc 
      tgcggaagg gcaaagacc tcttccaca agcagcact 
      cggacgaag tggacttgg cgcacaaca agaccaagt 
      tggccaaga tcgtggtcg agtgccgca aggagggga 
      ttgtgagct actccaccc cagagagct tggaccagc 
      ccggggggc cccccaagt gggagaagt gccggctgg 
      cgctggtca aggccgtcg gggggtaca tgctcgagt 
      tctactcgc cgcccaaaa gcaccaaac cccgtagtg 
      gcgtttttt gcgccctta tcaccgaag cccgcgaga 
      ctacggctc tggaaatgc cagatcatg aaaatactt 
      tcgttctta aggctaata atagtctcg aatttgtta 
      ttttggcca gagataccg atgatatga ggtcatggc 
      ttgctacaa ttaagtatt gtatacgtt cgggggcgg 
      gttacggtg acaccgccc ccggcgatc aacgcgact 
      atcactcgg acgcccccg aactgcccc cgagacatg 
      tctcaggcc gagggggcg accggttgt cttccagta 
      gtaacttcg atatgtgcc cctcaaccg gcgataata 
      tctcaatgg agactgata ttggccaga cgttaaaaa 
      aagaattct ggttccatg gcactctag gccggtccg 
      aagccacca atctagtcc tccatgacg gcgctagag 
      gccatggat tgtttctgg tacgtcaga gtgagacac 
      ggaccggcg aattcgtcc taactttca attttcaag 
      gccgggcta agcacctcc ggatgacca tcaacgacc 
      aaggccagt gccgagtgc aacacttct ggttccagt 
      ccatctacg aaatgctcg aacacttcc gacagcagc 
      cgattccgc tcgagagcg gcggcaatt ccgacgtca 
      cactcaccg acttcgttg tcaattccg ccgcaagag 
      ggcagctcc aaggctcga tggggcgga gctcgcaag 
      cctcgacca atggcgtgc acgaacgca gagtcccgc 
      ccatacccg aaatcagac aagtgagat aaaaatgga 
      tgtgttgta catattttt gtgacgagt gtgctttgc 
      tttactact taatttgta tatattttc gtgcaataa 
      gtgtacatt tttcaagta tttatttta cggttaaga 
      gactaaatt agtgatacg ttc
    • >TC015430 RB
      tgtgaaagc tgccgctta acgtcaaaa caataataa 
      atcaaccgc aaactatgt gattctgag caagtagtg 
      cccccttga agagtccaa tggcgagtg cggcggagc 
      tcccgtcgg ccgacaact cgtggatcg aattctgcg 
      agcggcatg ccagtgtcg ccgctcaag acttctcca 
      aaagttgcg tccagttca tgtcgttaa acctttcgg 
      aatcggcca aagcgacaa taacccaca aagacttcc 
      ttggcaaat tcctcgaga cgttcgccg agcacttcg 
      agaccgact tcggcaagc gcaaggccc aggtggcga 
      aaatcgcga atgggggct cccgcctcg acgacgacg 
      cctcggagc ccgaggagg gctccccga agatgcccc 
      acaagccgt tcttccgca ggttaagtt tcaaaatgc 
      tgcggaagg gcaaagacc tcttccaca agcagcact 
      cggacgaag tggacttgg cgcacaaca agaccaagt 
      tggccaaga tcgtggtcg agtgccgca aggagggga 
      ttgtgagct actccaccc cagagagct tggaccagc 
      ccggggggc cccccaagt gggagaagt gccggctgg 
      cgctggtca aggccgtcg gggggtaca tgctcgagt 
      tctactcgc cgcccaaaa gcaccaaac cccgtagtg 
      gcgtttttt gcgccctta tcaccgaag cccgcgaga 
      ctacggctc tggaaatgc cagatcatg aaaatactt 
      tcgttctta aggctaata atagtctcg aatttgtta 
      ttttggcca gagataccg atgatatga ggtcatggc 
      ttgctacaa ttaagtatt gtatacgtt cgggggcgg 
      gttacggtg acaccgccc ccggcgatc aacgcgact 
      atcactcgg acgcccccg aactgcccc cgagacatg 
      tctcaggcc gagggggcg accggttgt cttccagta 
      gtaacttcg atatgtgcc cctcaaccg gcgataata 
      tctcaatgg agactgata ttggccaga cgttaaaaa 
      aagaattct ggttccatg gcactctag gccggtccg 
      aagccacca atctagtcc tccatgacg gcgctagag 
      gccatggat tgtttctgg tacgtcaga gtgagacac 
      ggaccggcg aattcgtcc taactttca attttcaag 
      gccgggcta agcacctcc ggatgacca tcaacgacc 
      aaggccagt gccgagtgc aacacttct ggttccagt 
      ccatctacg aaatgctcg aacacttcc gacagcagc 
      cgattccgc tcgagagcg gcggcaatt ccgacgtca 
      cactcaccg acttcgttg tcaattccg ccgcaagag 
      ggcagctcc aaggctcga tggggcgga gctcgcaag 
      cctcgacca atggcgtgc acgaacgca gagtcccgc 
      ccatacccg aaatcagac aagtccaaa cacacaatg 
      ggagtgtgc gaactcagg aaaccactc tggaacaaa 
      tttccgagg cacgagctg ttgaaaatc agtacagtt 
      ttgtgtaaa ataacaaaa acgagtcag tattattaa 
      aatggccgc ctgtcactt tgacagccg gccacttga 
      ttggcaaaa tcaaataag tgtacccaa cccagtgta 
      atatatgtt atata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY