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Gene information
General information
TC015403 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid39: 403881..404899
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015403
    iBeetle screen iB_02473
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015403 RA
      MHFFGVYLL YCTNPKYRG RVYIGYTVD PNRRIKQHN 
      RGKQHGGAW KTSQKGPWS MVLIIHGFP NNISALRFE 
      WAWQHPHVS RRLAHVSKK NTKEKVYDF TIRVLSEML 
      QVGPWKRLP LTIRWLNDE FRRDLEKKP PDHMPICYG 
      PVISKKIPS VVTLSHETN FCYLCNDEV FNGMKCLNF 
      ECDLNAHVI CLSKCFLKG GDYVPVEGR CPKCCQPCL 
      WGDLVRRFK GYHNQDVEI TNLGDCE
    CDS sequence
    • >TC015403 RA
      atgcacttt ttcggggtt tatctgctc tattgcacc 
      aaccccaag tacaggggg cgcgtctac atcgggtac 
      acggtggac cccaaccgc cgcatcaag cagcacaac 
      cgcgggaag caacacggt ggcgcctgg aagaccagt 
      caaaagggg ccctggtcg atggttttg ataattcac 
      ggctttccg aacaatatc tcggctcta agatttgag 
      tgggcgtgg caacacccg cacgtttcg cgccgtttg 
      gcgcacgtt tcgaaaaaa aataccaaa gaaaaagtt 
      tacgatttt acgattagg gttttaagc gaaatgttg 
      caagtgggg ccttggaaa aggctcccg ctgacgatt 
      cgctggctc aatgatgaa ttcagacgc gatctagag 
      aaaaagcca ccggaccat atgccaatt tgttacggc 
      ccagttatt agtaaaaaa atcccaagt gttgtcaca 
      ctatcgcac gaaacgaat ttttgttat ttgtgtaat 
      gatgaggtt tttaatgga atgaagtgt ttgaatttt 
      gagtgcgat ttaaacgct catgttatt tgtttgtcc 
      aagtgtttt ctcaaaggg ggcgactac gtgccggtg 
      gaggggcgg tgccccaag tgttgccaa ccctgtctt 
      tggggggat ttagtccgc aggtttaag ggctaccac 
      aaccaggac gtggaaatc accaactta ggggattgt 
      gaataa
    mRNA sequence
    • >TC015403 RA
      acctcgaaa atgcacttt ttcggggtt tatctgctc 
      tattgcacc aaccccaag tacaggggg cgcgtctac 
      atcgggtac acggtggac cccaaccgc cgcatcaag 
      cagcacaac cgcgggaag caacacggt ggcgcctgg 
      aagaccagt caaaagggg ccctggtcg atggttttg 
      ataattcac ggctttccg aacaatatc tcggctcta 
      agatttgag tgggcgtgg caacacccg cacgtttcg 
      cgccgtttg gcgcacgtt tcgaaaaaa aataccaaa 
      gaaaaagtt tacgatttt acgattagg gttttaagc 
      gaaatgttg caagtgggg ccttggaaa aggctcccg 
      ctgacgatt cgctggctc aatgatgaa ttcagacgc 
      gatctagag aaaaagcca ccggaccat atgccaatt 
      tgttacggc ccagttatt agtaaaaaa atcccaagt 
      gttgtcaca ctatcgcac gaaacgaat ttttgttat 
      ttgtgtaat gatgaggtt tttaatgga atgaagtgt 
      ttgaatttt gagtgcgat ttaaacgct catgttatt 
      tgtttgtcc aagtgtttt ctcaaaggg ggcgactac 
      gtgccggtg gaggggcgg tgccccaag tgttgccaa 
      ccctgtctt tggggggat ttagtccgc aggtttaag 
      ggctaccac aaccaggac gtggaaatc accaactta 
      ggggattgt gaataaatt gctcaattc taatttaaa 
      ttataccga cttttacca cgattacaa agttggcaa 
      cactggcaa acgtgccgt taatggcca tttcattac 
      aacttatta ttattatta ttattacaa atatatatt 
      ttggtgatt t