Status
Please wait ...
Gene information
General information
TC015386 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid39: 134356..135810
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015386
    iBeetle screen iB_02472
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015386 RA
      MTAKELEKN FKIPKRKLN LDESAPVVA QVMENSYYN 
      YMVEPQPPV IAPIEVEAA RNLILQSLQ KRECERPKT 
      YQNHVLKET IPDCFDETL PRDLTSLFQ PLYCKLCSA 
      QLSSNLVAK LHYKSKNHE KKIRKFLID YSERTGEPL 
      HKRAKVAPG GDSDDGNPL YYYCDVCDL PLTGKLHAE 
      SHYLGKNHR KVTLGMKAP SAKGKTRKS SGNSDGFGA 
      DFHPVESVG FRCELCNIT TTCQEQLES HYNGQKHRK 
      KLKQQAMEG VPVGSPHDS ILTSVLTAD AGDCSVYRT 
      PSGQYYCQT CNCSSNSEV QFKQHLHSK NHLKKASQK 
      KA
    • >TC015386 RB
      MQTSEDGEY IPSKELEKN FKIPKRKLN LDESAPVVA 
      QVMENSYYN YMVEPQPPV IAPIEVEAA RNLILQSLQ 
      KRECERPKT YQNHVLKET IPDCFDETL PRDLTSLFQ 
      PLYCKLCSA QLSSNLVAK LHYKSKNHE KKIRKFLID 
      YSERTGEPL HKRAKVAPG GDSDDGNPL YYYCDVCDL 
      PLTGKLHAE SHYLGKNHR KVTLGMKAP SAKGKTRKS 
      SGNSDGFGA DFHPVESVG FRCELCNIT TTCQEQLES 
      HYNGQKHRK KLKQQAMEG VPVGSPHDS ILTSVLTAD 
      AGDCSVYRT PSGQYYCQT CNCSSNSEV QFKQHLHSK 
      NHLKKASQK KA
    CDS sequence
    • >TC015386 RA
      atgacagcg aaagagctg gaaaaaaac tttaaaata 
      ccaaaacgg aaattaaat ttggacgag agcgccccc 
      gtggtggct caagtaatg gaaaactca tactataac 
      tacatggtg gagccccaa ccgcctgtg atagcgccc 
      attgaggtg gaggcagct cgtaaccta attttgcaa 
      tccttgcaa aaacgcgaa tgtgaacgg ccaaaaacg 
      taccagaat catgtttta aaagagaca attccggat 
      tgttttgat gagacgctt ccgcgcgac ttaacctct 
      cttttccaa ccgttgtat tgcaaatta tgctcggcc 
      caacttagc agtaacctt gtggccaag ttacattac 
      aagtcgaag aaccatgag aaaaaaatt cgcaaattt 
      ttgattgat tattcggaa cggactggg gaaccgttg 
      cataagcgg gccaaggtg gccccaggg ggcgattcg 
      gatgatggc aaccccttg tattactac tgtgacgtg 
      tgtgattta ccccttacg gggaaattg cacgcagag 
      tctcattat ttgggaaaa aaccatcgc aaggtcact 
      ttgggaatg aaagcgcct tcggcgaag gggaaaaca 
      cgcaaaagt agtggtaac agtgatggt tttggggcc 
      gattttcac ccagtggag tccgtgggg ttccggtgc 
      gagttgtgc aatattaca acgacgtgt caggagcag 
      ttagagagt cactataac gggcagaag caccgcaaa 
      aagttgaaa caacaagct atggagggg gttccagtt 
      gggagcccc catgatagc attttgacg agtgtctta 
      acggcagat gcgggagat tgttctgtt tataggacc 
      ccctcagga cagtactac tgtcagacg tgtaattgc 
      tcgagtaat tccgaagtt caattcaag cagcattta 
      cacagcaag aatcatttg aagaaggcg agtcaaaag 
      aaggcgtaa
    • >TC015386 RB
      atgcaaacg agcgaagac ggtgaatat atcccgtcg 
      aaagagctg gaaaaaaac tttaaaata ccaaaacgg 
      aaattaaat ttggacgag agcgccccc gtggtggct 
      caagtaatg gaaaactca tactataac tacatggtg 
      gagccccaa ccgcctgtg atagcgccc attgaggtg 
      gaggcagct cgtaaccta attttgcaa tccttgcaa 
      aaacgcgaa tgtgaacgg ccaaaaacg taccagaat 
      catgtttta aaagagaca attccggat tgttttgat 
      gagacgctt ccgcgcgac ttaacctct cttttccaa 
      ccgttgtat tgcaaatta tgctcggcc caacttagc 
      agtaacctt gtggccaag ttacattac aagtcgaag 
      aaccatgag aaaaaaatt cgcaaattt ttgattgat 
      tattcggaa cggactggg gaaccgttg cataagcgg 
      gccaaggtg gccccaggg ggcgattcg gatgatggc 
      aaccccttg tattactac tgtgacgtg tgtgattta 
      ccccttacg gggaaattg cacgcagag tctcattat 
      ttgggaaaa aaccatcgc aaggtcact ttgggaatg 
      aaagcgcct tcggcgaag gggaaaaca cgcaaaagt 
      agtggtaac agtgatggt tttggggcc gattttcac 
      ccagtggag tccgtgggg ttccggtgc gagttgtgc 
      aatattaca acgacgtgt caggagcag ttagagagt 
      cactataac gggcagaag caccgcaaa aagttgaaa 
      caacaagct atggagggg gttccagtt gggagcccc 
      catgatagc attttgacg agtgtctta acggcagat 
      gcgggagat tgttctgtt tataggacc ccctcagga 
      cagtactac tgtcagacg tgtaattgc tcgagtaat 
      tccgaagtt caattcaag cagcattta cacagcaag 
      aatcatttg aagaaggcg agtcaaaag aaggcgtaa
    mRNA sequence
    • >TC015386 RA
      tgtcgaatt tttttaaat caataatta agtgttctc 
      cagtgttta aatcgaata aattcgcaa tgcaaacga 
      gcgaagacg gtgaatata tcccgtgta agtacccta 
      aagactccc tagcgctct taaactata aacctaacc 
      tcaactaat gacagcgaa agagctgga aaaaaactt 
      taaaatacc aaaacggaa attaaattt ggacgagag 
      cgcccccgt ggtggctca agtaatgga aaactcata 
      ctataacta catggtgga gccccaacc gcctgtgat 
      agcgcccat tgaggtgga ggcagctcg taacctaat 
      tttgcaatc cttgcaaaa acgcgaatg tgaacggcc 
      aaaaacgta ccagaatca tgttttaaa agagacaat 
      tccggattg ttttgatga gacgcttcc gcgcgactt 
      aacctctct tttccaacc gttgtattg caaattatg 
      ctcggccca acttagcag taaccttgt ggccaagtt 
      acattacaa gtcgaagaa ccatgagaa aaaaattcg 
      caaattttt gattgatta ttcggaacg gactgggga 
      accgttgca taagcgggc caaggtggc cccaggggg 
      cgattcgga tgatggcaa ccccttgta ttactactg 
      tgacgtgtg tgatttacc ccttacggg gaaattgca 
      cgcagagtc tcattattt gggaaaaaa ccatcgcaa 
      ggtcacttt gggaatgaa agcgccttc ggcgaaggg 
      gaaaacacg caaaagtag tggtaacag tgatggttt 
      tggggccga ttttcaccc agtggagtc cgtggggtt 
      ccggtgcga gttgtgcaa tattacaac gacgtgtca 
      ggagcagtt agagagtca ctataacgg gcagaagca 
      ccgcaaaaa gttgaaaca acaagctat ggagggggt 
      tccagttgg gagccccca tgatagcat tttgacgag 
      tgtcttaac ggcagatgc gggagattg ttctgttta 
      taggacccc ctcaggaca gtactactg tcagacgtg 
      taattgctc gagtaattc cgaagttca attcaagca 
      gcatttaca cagcaagaa tcatttgaa gaaggcgag 
      tcaaaagaa ggcgtaaaa gttttgtgt atgtatgaa 
      atactgtgt ttttttaga aaattgtgt tgaaaattt 
      gaaaattat gtgaaaaat tcagttatt cagttaatt 
      tagttattt tgaacaaaa aatgaccaa gttagttgt 
      ttaaatctt tatttgtgc aagaataca aatacctaa 
      aaaaa
    • >TC015386 RB
      tgtcgaatt tttttaaat caataatta agtgttctc 
      cagtgttta aatcgaata aattcgcaa tgcaaacga 
      gcgaagacg gtgaatata tcccgtcga aagagctgg 
      aaaaaaact ttaaaatac caaaacgga aattaaatt 
      tggacgaga gcgcccccg tggtggctc aagtaatgg 
      aaaactcat actataact acatggtgg agccccaac 
      cgcctgtga tagcgccca ttgaggtgg aggcagctc 
      gtaacctaa ttttgcaat ccttgcaaa aacgcgaat 
      gtgaacggc caaaaacgt accagaatc atgttttaa 
      aagagacaa ttccggatt gttttgatg agacgcttc 
      cgcgcgact taacctctc ttttccaac cgttgtatt 
      gcaaattat gctcggccc aacttagca gtaaccttg 
      tggccaagt tacattaca agtcgaaga accatgaga 
      aaaaaattc gcaaatttt tgattgatt attcggaac 
      ggactgggg aaccgttgc ataagcggg ccaaggtgg 
      ccccagggg gcgattcgg atgatggca accccttgt 
      attactact gtgacgtgt gtgatttac cccttacgg 
      ggaaattgc acgcagagt ctcattatt tgggaaaaa 
      accatcgca aggtcactt tgggaatga aagcgcctt 
      cggcgaagg ggaaaacac gcaaaagta gtggtaaca 
      gtgatggtt ttggggccg attttcacc cagtggagt 
      ccgtggggt tccggtgcg agttgtgca atattacaa 
      cgacgtgtc aggagcagt tagagagtc actataacg 
      ggcagaagc accgcaaaa agttgaaac aacaagcta 
      tggaggggg ttccagttg ggagccccc atgatagca 
      ttttgacga gtgtcttaa cggcagatg cgggagatt 
      gttctgttt ataggaccc cctcaggac agtactact 
      gtcagacgt gtaattgct cgagtaatt ccgaagttc 
      aattcaagc agcatttac acagcaaga atcatttga 
      agaaggcga gtcaaaaga aggcgtaaa agttttgtg 
      tatgtatga aatactgtg tttttttag aaaattgtg 
      ttgaaaatt tgaaaatta tgtgaaaaa ttcagttat 
      tcagttaat ttagttatt ttgaacaaa aaatgacca 
      agttagttg tttaaatct ttatttgtg caagaatac 
      aaataccta aaaaaa