Status
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Gene information
General information
TC015365 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 2..8410
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015365
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02469

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    Comment: Fluorescent spots/tube-like structure present.
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    cuticle everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    Fertility
    egg production: number <50% of expected -
    egg chamber not present - Number of animals showing the phenotype: 4
    tropharium structure irregular - Number of animals showing the phenotype: 4
    Comment: cells are smaller (not polyploid?!) and fewer
    tropharium size decreased - Number of animals showing the phenotype: 4

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015365 RA
      MFIYDMPFL ERKKLCSIL DRNNCFEEL AAVHMQFDS 
      ETVQKISRE FFRGISPTE KLLDLWSHH NHTVFELFI 
      LLYRMKHYQ AMTILKPLV DSKYHHLIK QSTCLPELM 
      ADLKLEQEP ITSEKILNA PSCENNETK PQKPNGISL 
      ISCVTESVG AVPQIPYDD LRQSTNDWN TGSILGRGG 
      FGTVFKGVW KCTQVAIKR IEKKADDNS DQIFQSYTE 
      LHCLNAYRH DNILPLYGY SIDGPQHCL VYQYMSGGS 
      LEHRLHIRD ASRVLSWPT RLTIAIGTA RGLQFLHTI 
      RDKPLIHGD IKSANILLD LNEIPRIGD FGLAREGPP 
      QTDYVKVSR IHGTRPYLP DEFMKYKKF STKVDTYSF 
      GVVLFELAT AGAAYSNKR VNKFLRDHV VFWEGDVLQ 
      LKDSRAEGG EDFFKGLVE VGKGCVRTG AKDRPEMVT 
      VLLQLEAVM NQ
    CDS sequence
    • >TC015365 RA
      atgtttatt tacgatatg ccgtttctc gaacgtaaa 
      aaactctgt agtattttg gatcggaat aattgcttt 
      gaggaatta gctgcggtt catatgcaa tttgattct 
      gagacggtc caaaaaatt agtcgcgaa tttttcaga 
      ggcatttcc ccaactgaa aaactcctc gacttatgg 
      agtcatcac aaccatacc gtttttgaa ttgtttatt 
      ctcttgtat cgaatgaaa cattatcaa gccatgaca 
      atccttaaa ccacttgtc gattcaaaa tatcatcat 
      ttgattaaa caaagtact tgtcttcct gaattaatg 
      gccgattta aaattagaa caagagcca ataacaagt 
      gaaaaaata ctaaacgct ccaagttgt gaaaacaat 
      gaaacaaag ccccaaaaa ccaaacgga atctcgctt 
      atttcttgt gtgacggaa tctgtggga gctgttcct 
      caaattccc tatgatgat ttaagacag tcaacaaat 
      gattggaac actggatcg attctgggt cgtggaggc 
      tttgggacg gtatttaag ggtgtttgg aagtgcact 
      caagttgct attaaaaga attgagaaa aaagcggat 
      gacaattcg gatcaaatt tttcaatcc tatacagaa 
      ttacattgt ttgaatgca tacagacac gataacatt 
      ttgccactt tacgggtat agcattgac ggacctcag 
      cattgtttg gtataccaa tacatgtcc gggggttcc 
      ctcgagcac cgtctccac attcgtgat gcgtcacgt 
      gtcctttca tggccaact cgtctcaca atcgcaatt 
      ggaaccgct cgaggtctt caatttcta cacacaatt 
      cgtgacaaa ccgcttatt catggcgac atcaagtct 
      gccaacatt cttctcgac ctaaacgaa atccctaga 
      attggggat tttggttta gctcgtgaa gggccacca 
      caaaccgat tatgttaaa gtgagtcgt attcatgga 
      acgaggccg tatcttcct gacgaattt atgaaatat 
      aaaaaattt tcgaccaaa gttgatact tacagtttt 
      ggagttgtt ttattcgaa ttggcgaca gctggagct 
      gcttattca aataaaagg gtgaacaag tttttgaga 
      gatcatgtc gtgttttgg gaaggtgat gtgttacaa 
      ttgaaagat tcaagggct gaaggagga gaagatttt 
      tttaaggga ttagtggaa gttggaaaa gggtgtgtg 
      aggacgggg gcaaaggac cgaccggaa atggttacg 
      gttttattg caattagaa gctgtgatg aatcaataa
    mRNA sequence
    • >TC015365 RA
      aaaaaaatt cataactca aaaactaaa agtcgtaga 
      gcaatgcgg aaaattaca aagtttgac atgtagtga 
      tttagtgag aataatgat ttttcgaaa atgttgttt 
      ttgtgtgcc tttacggtg tggtgctgc tatgttact 
      tgtcctcca aatggcatg tttgacgcc ttatacgat 
      atacaatcc caccgcaaa actaatttt catctcaat 
      aggaatcat gatcctttt ggcaacagc tgcagtgat 
      atttatgtg ggctattgt atgggaaaa acctaaata 
      tctttatgc aacacttgg attatcact gggatccat 
      catttttta ttttgccac aatagatta cgactattc 
      ttttaagac atcaacaac acccacaac aaagaaaga 
      aggaactgt tcatttaat cctcttgat taataacac 
      cacaggaat attaacata tttttattt tatttttat 
      gttatacaa tacatatgt taagttaca acataaagc 
      tagaaaaaa acaacaatc tactgtact accacaata 
      aaccatttg ttaatgtgt ccggttttg tctttttac 
      cacggtcgg actttgcct tgagttgcg tgagttaaa 
      atccatgta tttttcggg tctttaacc attgacatt 
      tgtttatgt ctaccgtga gttttacaa aggacataa 
      agacgttgc tctcgcatc acaaaatag cagtcttta 
      caacgtcta tgcagcttt ccttttact ttgaaacca 
      caacactgt gtttggttg taattcaca gcggctaaa 
      agtttgctc ggtgatgtt gcaatcgac ggcgtaaat 
      atgtgataa ttcttggaa gttgcaaca acaacaaag 
      gggcacttt ctaatcaca ttaattaca tttcgaatg 
      agtggaaac attttgtga aagatgagg ttagaaaac 
      cgcttaggg atttatcat tagaaaagt tactaggat 
      tggtgacag acactgctg actgtcact gcttgtcaa 
      tgtaacata acccaattt ttttattgt tattttttt 
      taacgatgt ttatttacg atatgccgt ttctcgaac 
      gtaaaaaac tctgtagta ttttggatc ggaataatt 
      gctttgagg aattagctg cggttcata tgcaatttg 
      attctgaga cggtccaaa aaattagtc gcgaatttt 
      tcagaggca tttccccaa ctgaaaaac tcctcgact 
      tatggagtc atcacaacc ataccgttt ttgaattgt 
      ttattctct tgtatcgaa tgaaacatt atcaagcca 
      tgacaatcc ttaaaccac ttgtcgatt caaaatatc 
      atcatttga ttaaacaaa gtacttgtc ttcctgaat 
      taatggccg atttaaaat tagaacaag agccaataa 
      caagtgaaa aaatactaa acgctccaa gttgtgaaa 
      acaatgaaa caaagcccc aaaaaccaa acggaatct 
      cgcttattt cttgtgtga cggaatctg tgggagctg 
      ttcctcaaa ttccctatg atgatttaa gacagtcaa 
      caaatgatt ggaacactg gatcgattc tgggtcgtg 
      gaggctttg ggacggtat ttaagggtg tttggaagt 
      gcactcaag ttgctatta aaagaattg agaaaaaag 
      cggatgaca attcggatc aaatttttc aatcctata 
      cagaattac attgtttga atgcataca gacacgata 
      acattttgc cactttacg ggtatagca ttgacggac 
      ctcagcatt gtttggtat accaataca tgtccgggg 
      gttccctcg agcaccgtc tccacattc gtgatgcgt 
      cacgtgtcc tttcatggc caactcgtc tcacaatcg 
      caattggaa ccgctcgag gtcttcaat ttctacaca 
      caattcgtg acaaaccgc ttattcatg gcgacatca 
      agtctgcca acattcttc tcgacctaa acgaaatcc 
      ctagaattg gggattttg gtttagctc gtgaagggc 
      caccacaaa ccgattatg ttaaagtga gtcgtattc 
      atggaacga ggccgtatc ttcctgacg aatttatga 
      aatataaaa aattttcga ccaaagttg atacttaca 
      gttttggag ttgttttat tcgaattgg cgacagctg 
      gagctgctt attcaaata aaagggtga acaagtttt 
      tgagagatc atgtcgtgt tttgggaag gtgatgtgt 
      tacaattga aagattcaa gggctgaag gaggagaag 
      attttttta agggattag tggaagttg gaaaagggt 
      gtgtgagga cgggggcaa aggaccgac cggaaatgg 
      ttacggttt tattgcaat tagaagctg tgatgaatc 
      aataaattt ttacataca ttttttaca attgtttca 
      tttctctaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY