Status
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Gene information
General information
TC033299
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC015361,TC015363,TC015362
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 22107..32804
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033299
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02468

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: 4 - Number of animals showing the phenotype: 4
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    head shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    legs shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033299 RA
      MWQQSPPFT YSNPQSRIE FAHHTSCGH AASIPTSCS 
      RYLLSSLRF GHVGKVVSS TTRLQVSSA RAGAAQQPP 
      CLCSTTAPI VGGQGTIES RALPLVDTA KHARDVVVL 
      VVVCESVVG RFVLQRVPW TSHTGCSVK MSLNTLIEA 
      AKFLEMQEQ KEQQQHHQV AGPSKTSPP VHHKLSTVL 
      ITSPNLLTL TQETAKKAA APQAEQQQV VSSSSTVTA 
      APPPQPPRR ILHNSFDIM NPLVIDETN AEVKRKQPP 
      LVFRAGTRE VHNKLEKHR RAHLKECFD VLKKQLPQT 
      QDEKKTSNL SILHSALRC IQSLKRKER ELEHEMERL 
      ARDKIAAQQ KLAVLKKDI SSHYDNVDF SKILPEVVP 
      VTTDEVVKL ETVSSTSQN GAGGERIQI PLLTNKTQI 
      SIATTQVGT IKEVSQQPV QTTALSVLP VGYSVPQGL 
      VLQKLIVPK GITELTPLV STHFITQQQ INGKVVPLV 
      NAQYVVKPV VVVLLANKL DGEIRMSYT AVVSPLFVS 
      FITLILMSF SAKGGNRWW FGMRKDFCA FVLGACPLL 
      QEYANIAYH SERRTNGVE LQTQSDKSD PTNRLTAAG 
      ENYGSLMLQ IDFIIKSPN GTKTIHSVA KTVPPNELI 
      QKIFNTPES FKNEIAFYK KVVPTLEKF EQDQEISTT 
      GIANLFPKY YTSRINLNN STDNKVDSD AILLLENLK 
      FENFATVER TQGFNLQVS QVIVKHLAY FHALSLGLK 
      LKQPQIFHE KIQKYLAPS IPDKDVRII ETLAKLIEE 
      TPGCSLYVQ KARQILMKM LEPAPPPRE PFATFTHDD 
      FWVNNILFK QSTNEVKFI DFQRPRYAS PVRDLIFFL 
      FTSVENHVL ESSCDDLLQ LYYENFVSV LQRELKCDD 
      IEFTIETFQ QELKLEARN TQFGHIVFM LNPIFAPKG 
      SVKELEEMT VEDFLKSLE GGSDLHKAK LELVVGEFA 
      RRDWI
    CDS sequence
    • >TC033299 RA
      atgtggcaa cagagccca cctttcacg tactctaac 
      ccccaatcg cgcatcgaa tttgcgcac cacacaagc 
      tgtgggcac gcggcctcc atccccacc tcctgcagc 
      agatacctt ctttcttcc cttcgtttc ggtcacgta 
      ggcaaggtc gtgtcctcc accacaagg ctccaggtg 
      tcgtctgca agggctggt gcagcacag cagccccct 
      tgcttgtgt tccaccacc gccccaatc gtcggcggc 
      cagggaaca atcgaatca cgcgcactc ccattggtc 
      gatacagcc aaacacgca cgtgatgtg gtggttctt 
      gttgtagtg tgcgagagt gtcgtcggt cgtttcgta 
      ctacaacga gtgccctgg acttcacac accggctgc 
      agtgtcaaa atgagcttg aacacctta atcgaagca 
      gcaaaattt ttggaaatg caagaacag aaagagcag 
      caacagcat catcaggtg gcggggcct tcaaagact 
      tcgcccccc gttcaccat aaattgtca acagttttg 
      attacatcg ccaaattta ctcactctg acgcaagaa 
      acggccaaa aaggccgca gccccccaa gctgaacag 
      caacaagta gtgtcgtct tctagtacc gtaacagca 
      gcaccacca ccgcagcct cctcgacgc atccttcac 
      aacagtttc gatataatg aaccccctt gtcatcgac 
      gagacaaac gccgaagtg aaacgaaaa cagccccca 
      ttggtgttt cgtgccggc accagagaa gtgcacaac 
      aaattggag aaacatcga cgtgcacat ctcaaagag 
      tgcttcgat gtgttgaaa aagcaactg ccacaaaca 
      caagacgaa aaaaaaacc tccaatttg agtatttta 
      cattcggcg ttgcgatgc attcagtcg ttgaaacgc 
      aaagaacgc gaattagaa cacgagatg gaaagatta 
      gcaagagat aaaattgcc gctcagcaa aagttggct 
      gtgttaaaa aaggatatt tcctcgcat tatgataat 
      gtggatttt tccaaaatt ttaccggaa gttgttcca 
      gttacaacc gatgaggtt gttaaactc gaaacggtt 
      agtagtacg agtcaaaat ggggcagga ggcgaaaga 
      atacagatt ccgcttttg acgaataaa acgcaaatt 
      tctatcgca acaactcaa gttggaaca atcaaagag 
      gtgagtcag caaccggtc cagacaaca gctttgtcg 
      gtacttcct gtcggttat tcagtacct caaggttta 
      gttttgcaa aaactcatc gttccgaaa ggcataacc 
      gaactgacg cctctcgta tcaacacat ttcatcacc 
      caacagcaa ataaacggc aaagttgtt ccattggta 
      aacgcccaa tacgtggtc aaaccagtg gtcgttgta 
      ttattagcc aataaattg gatggggag attcgtatg 
      tcgtacact gcagttgtt agtcccctg tttgtctca 
      tttattact ttaattttg atgtctttt agtgccaaa 
      gggggcaac cgatggtgg ttcggtatg agaaaagac 
      ttttgtgct ttcgttcta ggggcgtgc ccccttctt 
      caggaatat gccaatatt gcctatcac agcgaacgg 
      cgcaccaat ggtgttgaa ttacagacg caaagtgat 
      aagagtgac ccaacaaat cgtttgaca gcagcgggc 
      gaaaattac ggaagtctc atgttacaa atcgatttt 
      atcatcaaa tcaccgaat ggtaccaaa accattcat 
      tccgtagcc aaaactgtg cctcccaac gaattaatc 
      cagaaaatt ttcaatacg ccagagtca tttaagaat 
      gaaatagcc ttttacaag aaggtggta ccaactttg 
      gaaaaattt gaacaagat caagaaatc tctaccact 
      ggtattgca aatcttttt ccgaaatat tacacaagt 
      cgcattaat ttaaacaat agtaccgat aataaagtt 
      gattcggac gcaattctc ctccttgaa aatctcaaa 
      tttgaaaat ttcgctaca gttgaacgc actcaagga 
      ttcaatttg caagtatca caagtgatt gtgaaacat 
      ttggcctat tttcacgcc ttatcacta ggcctcaag 
      ctaaagcaa ccgcaaatt ttccacgag aaaattcaa 
      aagtatctt gcaccgtcc atcccagac aaggacgtt 
      cgaatcatt gaaacgctc gcaaaattg atagaagaa 
      acgcctggt tgtagtctt tacgttcag aaagcacgt 
      caaatcctt atgaagatg ttagaacct gcaccacct 
      cctcgtgaa ccttttgcc accttcacc catgacgat 
      ttctgggtc aacaacatt cttttcaaa caatccaca 
      aatgaagtc aaatttatc gattttcaa cgacccaga 
      tacgcttca ccggtccgc gatttgatt ttttttctt 
      tttacgagt gttgaaaat cacgttttg gagtcaagt 
      tgtgacgat ttgcttcaa ctctactac gaaaatttt 
      gtttcggta ctccaacgc gaactaaaa tgcgatgat 
      attgagttt actattgaa acattccaa caagagtta 
      aaattggag gctaggaat acacaattc ggacacatt 
      gtgtttatg ttgaatccc atttttgca ccaaaagga 
      agtgtcaag gagttggaa gagatgaca gtggaggat 
      ttcctcaag agtttggag gggggcagt gatctgcac 
      aaggccaag ctggagctg gttgttgga gagtttgcg 
      agaagagac tggatataa
    mRNA sequence
    • >TC033299 RA
      tagttgtaa gaaattgtg ttaaatgtg gcaacagag 
      cccaccttt cacgtactc taaccccca atcgcgcat 
      cgaatttgc gcaccacac aagctgtgg gcacgcggc 
      ctccatccc cacctcctg cagcagata ccttctttc 
      ttcccttcg tttcggtca cgtaggcaa ggtcgtgtc 
      ctccaccac aaggctcca ggtgtcgtc tgcaagggc 
      tggtgcagc acagcagcc cccttgctt gtgttccac 
      caccgcccc aatcgtcgg cggccaggg aacaatcga 
      atcacgcgc actcccatt ggtcgatac agccaaaca 
      cgcacgtga tgtggtggt tcttgttgt agtgtgcga 
      gagtgtcgt cggtcgttt cgtactaca acgagtgcc 
      ctggacttc acacaccgg ctgcagtgt caaaatgag 
      cttgaacac cttaatcga agcagcaaa atttttgga 
      aatgcaaga acagaaaga gcagcaaca gcatcatca 
      ggtggcggg gccttcaaa gacttcgcc ccccgttca 
      ccataaatt gtcaacagt tttgattac atcgccaaa 
      tttactcac tctgacgca agaaacggc caaaaaggc 
      cgcagcccc ccaagctga acagcaaca agtagtgtc 
      gtcttctag taccgtaac agcagcacc accaccgca 
      gcctcctcg acgcatcct tcacaacag tttcgatat 
      aatgaaccc ccttgtcat cgacgagac aaacgccga 
      agtgaaacg aaaacagcc cccattggt gtttcgtgc 
      cggcaccag agaagtgca caacaaatt ggagaaaca 
      tcgacgtgc acatctcaa agagtgctt cgatgtgtt 
      gaaaaagca actgccaca aacacaaga cgaaaaaaa 
      aacctccaa tttgagtat tttacattc ggcgttgcg 
      atgcattca gtcgttgaa acgcaaaga acgcgaatt 
      agaacacga gatggaaag attagcaag agataaaat 
      tgccgctca gcaaaagtt ggctgtgtt aaaaaagga 
      tatttcctc gcattatga taatgtgga tttttccaa 
      aattttacc ggaagttgt tccagttac aaccgatga 
      ggttgttaa actcgaaac ggttagtag tacgagtca 
      aaatggggc aggaggcga aagaataca gattccgct 
      tttgacgaa taaaacgca aatttctat cgcaacaac 
      tcaagttgg aacaatcaa agaggtgag tcagcaacc 
      ggtccagac aacagcttt gtcggtact tcctgtcgg 
      ttattcagt acctcaagg tttagtttt gcaaaaact 
      catcgttcc gaaaggcat aaccgaact gacgcctct 
      cgtatcaac acatttcat cacccaaca gcaaataaa 
      cggcaaagt tgttccatt ggtaaacgc ccaatacgt 
      ggtcaaacc agtggtcgt tgtattatt agccaataa 
      attggatgg ggagattcg tatgtcgta cactgcagt 
      tgttagtcc cctgtttgt ctcatttat tactttaat 
      tttgatgtc ttttagtgc caaaggggg caaccgatg 
      gtggttcgg tatgagaaa agacttttg tgctttcgt 
      tctaggggc gtgccccct tcttcagga atatgccaa 
      tattgccta tcacagcga acggcgcac caatggtgt 
      tgaattaca gacgcaaag tgataagag tgacccaac 
      aaatcgttt gacagcagc gggcgaaaa ttacggaag 
      tctcatgtt acaaatcga ttttatcat caaatcacc 
      gaatggtac caaaaccat tcattccgt agccaaaac 
      tgtgcctcc caacgaatt aatccagaa aattttcaa 
      tacgccaga gtcatttaa gaatgaaat agcctttta 
      caagaaggt ggtaccaac tttggaaaa atttgaaca 
      agatcaaga aatctctac cactggtat tgcaaatct 
      ttttccgaa atattacac aagtcgcat taatttaaa 
      caatagtac cgataataa agttgattc ggacgcaat 
      tctcctcct tgaaaatct caaatttga aaatttcgc 
      tacagttga acgcactca aggattcaa tttgcaagt 
      atcacaagt gattgtgaa acatttggc ctattttca 
      cgccttatc actaggcct caagctaaa gcaaccgca 
      aattttcca cgagaaaat tcaaaagta tcttgcacc 
      gtccatccc agacaagga cgttcgaat cattgaaac 
      gctcgcaaa attgataga agaaacgcc tggttgtag 
      tctttacgt tcagaaagc acgtcaaat ccttatgaa 
      gatgttaga acctgcacc acctcctcg tgaaccttt 
      tgccacctt cacccatga cgatttctg ggtcaacaa 
      cattctttt caaacaatc cacaaatga agtcaaatt 
      tatcgattt tcaacgacc cagatacgc ttcaccggt 
      ccgcgattt gattttttt tctttttac gagtgttga 
      aaatcacgt tttggagtc aagttgtga cgatttgct 
      tcaactcta ctacgaaaa ttttgtttc ggtactcca 
      acgcgaact aaaatgcga tgatattga gtttactat 
      tgaaacatt ccaacaaga gttaaaatt ggaggctag 
      gaatacaca attcggaca cattgtgtt tatgttgaa 
      tcccatttt tgcaccaaa aggaagtgt caaggagtt 
      ggaagagat gacagtgga ggatttcct caagagttt 
      ggagggggg cagtgatct gcacaaggc caagctgga 
      gctggttgt tggagagtt tgcgagaag agactggat 
      ataaatagt ttaaacaaa atagcagtt gaccatcaa 
      accatttat ttgccaaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY