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Gene information
General information
TC015347 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid39: 123269..127824
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC015347
    iBeetle screen iB_02467
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015347 RA
      METKLCRIC GKLGQNYVN IFRTEGLKN KIETCLPII 
VSPHSLLPD TICGECSDN VDNFYSFIK NCLQNIIIL 
EAQYDINES CLKTKRKHD KGCTTDLAR KSDKNTQTI 
DYLDLLLKE NELNFPNLK KLVDYDDSD SEQDVKKAA 
SIFDNFGRK YFDDAENYL IDEITQRKK RKNEDTIES 
SRAKICKIE TTNRRKNKQ PKKLEIKND FLGDLPQVC 
LLCDEQFTG PSMLASHVF ETHGIDMAA VTGDTNDKK 
KKLPNLLKI SDLRKSDSI EEEEPPTLL PSFVCSLCP 
AMFTSRPDL FGHLRVKHS DQSALLCGM CLYQASSLA 
NLQSHVELC SHQHPTTTK YVCQVCRYG DDNSKSLEN 
HILVHDFLL DACKKQSKM FDPADYIET NGNYESGGS 
SPMTKSLAC SECGMDDFD NFKEFSTHR RSQHSIFHC 
DLCNKFYGR NSHLWKHVN RLHKGHPSI TCQLCYKTS 
ASKYHLAQH FNKIHSTKS CKKSENQLN FDNTDDFMS 
QKFQAFDFQ SVKQSFMRQ ELLEQQEKK FSENDDNSN 
NHLSDQEPP SAPKEIDSS SDLYTNIIT NYTPPANEG 
EFKCPKCGK GFHKKTLLK KHKKNCRPR LQKDLLTRC 
KSCARIFKD RQSLAKHLV NYHSEYACE ICGQKVQSK 
CEIVSHIRF THPGCHLFC TICGNILRS RNDMADHTR 
DHMNSYVCQ FCADALPSK IKLKMHILS LHRKILSLS 
CGVCLKLFE TQHVLRDHV RLVHRDQLA PLTSCPVCG 
KNYGSKWKT YDHLNKSHG RIFKACKAC LEVFDNENQ 
LQAHYDSVH GGGKNNIPV VTSQVSQPQ NHCESESER 
ETNSEQSDE QEEDEQYDN KISLLEKRL LGKNEKVLP 
TKINNKKLK ELDFDTNPQ HNSSKRTVY VNSNDPSFC 
EICHKTWPA KKHLWQHYI RCHKSVAAT VCGICLKTN 
SSYGALQAH LMETHPTLL HGQGFGSNF ICRICGRYH 
NASSKLKLH MAIHENFDW SMLEQPKEE KKEENGFQD 
ASEINYESL IEQVECSSG SESENVETK DKESSSEDD 
SDSETNEAS SVSEEEESS GSWFMVKSE ESEEEKLES 
AIRSIKYEC VEKVEEEVE EEYCDMQAQ SLNDNEIES 
AVGSIL
    CDS sequence
    • >TC015347 RA
      atggagacg aaactgtgc cgaatatgc ggaaaactc 
ggccaaaac tacgtgaat atttttcga accgaaggc 
ctgaagaac aagatcgaa acatgtctc cctattatt 
gtgtcgccc cattcactt ctccccgat acgatttgt 
ggcgaatgc tcagacaat gtggacaat ttctacagt 
tttatcaag aattgtctc cagaatatt atcatactt 
gaagcccaa tatgacata aacgagtct tgtttgaag 
acaaaacgc aaacatgac aaaggatgc accactgat 
ttggccaga aaaagcgac aaaaatact caaactatc 
gattatctt gatctcctc ctcaaagaa aacgaattg 
aattttcct aaccttaaa aaactggtc gactatgac 
gactctgat tctgaacaa gacgtcaaa aaagcagcg 
tcgatcttt gataatttt ggccgaaaa tatttcgac 
gatgcagaa aactacctt atcgacgaa attactcaa 
cgtaaaaag cgcaaaaac gaagataca atcgaatcg 
tcacgtgca aaaatctgt aaaatcgaa acaaccaat 
cggcggaaa aacaaacaa ccgaaaaaa ctcgaaatc 
aagaacgat tttctagga gatttaccg caagtttgt 
ttattatgc gatgaacaa ttcactggt ccttccatg 
ttggctagt cacgtgttt gaaacgcac ggcattgac 
atggcggcc gttaccggc gatactaac gacaaaaag 
aaaaagtta ccaaatcta ctcaagatt tcagatctc 
aggaaaagc gattcgatc gaagaagag gagccgccg 
actcttctc ccgagcttc gtttgttcc ctttgtccg 
gcgatgttt acatcacgt ccggacttg tttggccat 
cttcgtgtg aaacattcc gaccaatcg gctctctta 
tgtggcatg tgtctctat caagcttca tcattggct 
aatcttcag tcacatgtt gaattatgt tcccaccaa 
catccaacc acaacaaaa tatgtctgt caggtgtgt 
cgttatggt gatgataat tccaaatca ttggaaaat 
catatactc gtccatgac tttttgttg gatgcgtgt 
aaaaaacaa tcgaaaatg ttcgatcca gccgactat 
atcgaaacg aatggtaat tacgaatcg ggtggttcg 
tcaccaatg acaaaaagt ctagcgtgt agtgagtgc 
ggaatggac gattttgat aattttaaa gaatttagt 
acgcataga agaagccaa cattcaatt tttcattgc 
gatctttgt aataaattt tacggccgt aattcgcat 
ttatggaaa catgtcaat cgattgcat aaaggacat 
ccaagtatc acgtgtcag ttgtgctac aaaacgagt 
gcttcaaag tatcatctc gcacaacat ttcaataaa 
atccacagt acgaagagc tgcaaaaag agcgaaaat 
caattgaat tttgacaat acggacgat tttatgtca 
cagaaattc caagctttt gattttcaa tcggtcaaa 
cagagtttc atgcgacag gaattactc gaacaacag 
gaaaagaag ttttccgaa aacgacgac aactcgaat 
aatcattta tccgatcag gaaccacca tctgcacct 
aaagaaatt gactcatct agtgacctc tacactaac 
attattaca aactatacg ccacctgcc aacgaaggc 
gaattcaaa tgtcccaag tgtggtaaa ggttttcac 
aagaaaacc ctccttaag aaacacaaa aagaactgt 
agaccacgt ctccagaaa gatttactc actcgttgc 
aaatcatgt gctcgtata tttaaagat cgccaaagt 
ctcgccaaa catttggtt aattaccat tcagagtat 
gcgtgtgaa atatgcggt caaaaagtc caaagtaag 
tgtgagatt gtctcacat attaggttc acacatccc 
ggttgtcat ttgttttgt acgatatgt ggtaacata 
ttacgtagt cgtaacgat atggccgac cacacacgt 
gaccatatg aatagttac gtctgtcaa ttctgcgct 
gatgcgtta ccaagcaaa atcaaactt aaaatgcac 
attttaagt ctacatcgt aaaatatta agtttaagt 
tgcggtgtt tgtttgaaa ttgtttgaa acgcaacat 
gttctacgc gaccatgtc aggttagta catcgtgat 
cagttggcg ccactgact tcgtgtccc gtctgtggt 
aaaaattac ggttcaaaa tggaaaacc tacgatcac 
ttgaataag tcacacgga cgtattttt aaagcttgt 
aaggcttgt cttgaagtt tttgacaat gaaaatcaa 
ttacaagcc cactatgat agtgtacat ggtgggggt 
aaaaacaat ataccggtc gtaacgtca caagttagc 
caaccacaa aatcactgt gagagtgaa agtgagcga 
gaaacgaat agcgaacaa agtgatgag caagaagaa 
gacgaacaa tacgataat aaaatctca ctattggag 
aaacgtcta cttgggaaa aatgagaag gtgttacca 
accaaaatt aataataaa aaactcaaa gaactcgat 
tttgacacg aatccgcaa cataacagt tcaaaacgt 
acagtttac gtcaatagt aacgatccg tcattttgc 
gagatatgt cacaagaca tggcctgct aagaaacat 
ctctggcaa cactatatc cggtgtcat aaatcagtg 
gcggctacg gtttgtggc atttgtctc aaaacaaat 
tcctcctat ggtgcactt caagcccac ttgatggaa 
actcatcca acgcttttg cacggtcaa ggtttcggt 
tcgaatttt atttgtcgt atttgtggt cgttatcac 
aatgcgtca agtaaatta aaattgcat atggcgatt 
catgagaat tttgattgg tcgatgctt gaacaaccg 
aaagaggag aagaaggaa gagaatggg ttccaggat 
gcgtctgaa attaattac gagagtttg attgaacaa 
gtcgagtgt tcctcgggt tcggaaagt gaaaatgtc 
gaaacgaaa gataaggaa tcaagttcg gaggatgat 
agtgatagt gagacgaac gaagcgagt tctgtgagt 
gaggaagaa gagtcgagt ggaagttgg tttatggtt 
aaaagtgaa gagagtgag gaggaaaag ttggaatcg 
gcgatcaga tcgattaaa tatgagtgc gtggaaaag 
gtagaggag gaggtggag gaggaatat tgcgatatg 
caagcgcaa agtttgaat gataatgaa atcgagtcg 
gcggtgggg agcatattg taa
    mRNA sequence
    • >TC015347 RA
      tcgagtggg ttttgtttg ttggtttta acgtaaaaa 
ttaattcgg gagtagttt taatacacg gccagtgtt 
ttagttgtg tttctcgtt ttgttgggg gtggaaaag 
ccggtgaaa cgccgtcta gtgtgcaaa attgacact 
aaaatcagc aatggcgtc gttgtgttt gtgctcgaa 
ttgttgaaa attgcaaat ttcggtgca tcaaagcga 
tgaatgtat ttttgatgc gttaagagc cccctttgt 
ctttgaggc tccaggggg ccccgctat gcacgagaa 
tggagacga aactgtgcc gaatatgcg gaaaactcg 
gccaaaact acgtgaata tttttcgaa ccgaaggcc 
tgaagaaca agatcgaaa catgtctcc ctattattg 
tgtcgcccc attcacttc tccccgata cgatttgtg 
gcgaatgct cagacaatg tggacaatt tctacagtt 
ttatcaaga attgtctcc agaatatta tcatacttg 
aagcccaat atgacataa acgagtctt gtttgaaga 
caaaacgca aacatgaca aaggatgca ccactgatt 
tggccagaa aaagcgaca aaaatactc aaactatcg 
attatcttg atctcctcc tcaaagaaa acgaattga 
attttccta accttaaaa aactggtcg actatgacg 
actctgatt ctgaacaag acgtcaaaa aagcagcgt 
cgatctttg ataattttg gccgaaaat atttcgacg 
atgcagaaa actacctta tcgacgaaa ttactcaac 
gtaaaaagc gcaaaaacg aagatacaa tcgaatcgt 
cacgtgcaa aaatctgta aaatcgaaa caaccaatc 
ggcggaaaa acaaacaac cgaaaaaac tcgaaatca 
agaacgatt ttctaggag atttaccgc aagtttgtt 
tattatgcg atgaacaat tcactggtc cttccatgt 
tggctagtc acgtgtttg aaacgcacg gcattgaca 
tggcggccg ttaccggcg atactaacg acaaaaaga 
aaaagttac caaatctac tcaagattt cagatctca 
ggaaaagcg attcgatcg aagaagagg agccgccga 
ctcttctcc cgagcttcg tttgttccc tttgtccgg 
cgatgttta catcacgtc cggacttgt ttggccatc 
ttcgtgtga aacattccg accaatcgg ctctcttat 
gtggcatgt gtctctatc aagcttcat cattggcta 
atcttcagt cacatgttg aattatgtt cccaccaac 
atccaacca caacaaaat atgtctgtc aggtgtgtc 
gttatggtg atgataatt ccaaatcat tggaaaatc 
atatactcg tccatgact ttttgttgg atgcgtgta 
aaaaacaat cgaaaatgt tcgatccag ccgactata 
tcgaaacga atggtaatt acgaatcgg gtggttcgt 
caccaatga caaaaagtc tagcgtgta gtgagtgcg 
gaatggacg attttgata attttaaag aatttagta 
cgcatagaa gaagccaac attcaattt ttcattgcg 
atctttgta ataaatttt acggccgta attcgcatt 
tatggaaac atgtcaatc gattgcata aaggacatc 
caagtatca cgtgtcagt tgtgctaca aaacgagtg 
cttcaaagt atcatctcg cacaacatt tcaataaaa 
tccacagta cgaagagct gcaaaaaga gcgaaaatc 
aattgaatt ttgacaata cggacgatt ttatgtcac 
agaaattcc aagcttttg attttcaat cggtcaaac 
agagtttca tgcgacagg aattactcg aacaacagg 
aaaagaagt tttccgaaa acgacgaca actcgaata 
atcatttat ccgatcagg aaccaccat ctgcaccta 
aagaaattg actcatcta gtgacctct acactaaca 
ttattacaa actatacgc cacctgcca acgaaggcg 
aattcaaat gtcccaagt gtggtaaag gttttcaca 
agaaaaccc tccttaaga aacacaaaa agaactgta 
gaccacgtc tccagaaag atttactca ctcgttgca 
aatcatgtg ctcgtatat ttaaagatc gccaaagtc 
tcgccaaac atttggtta attaccatt cagagtatg 
cgtgtgaaa tatgcggtc aaaaagtcc aaagtaagt 
gtgagattg tctcacata ttaggttca cacatcccg 
gttgtcatt tgttttgta cgatatgtg gtaacatat 
tacgtagtc gtaacgata tggccgacc acacacgtg 
accatatga atagttacg tctgtcaat tctgcgctg 
atgcgttac caagcaaaa tcaaactta aaatgcaca 
ttttaagtc tacatcgta aaatattaa gtttaagtt 
gcggtgttt gtttgaaat tgtttgaaa cgcaacatg 
ttctacgcg accatgtca ggttagtac atcgtgatc 
agttggcgc cactgactt cgtgtcccg tctgtggta 
aaaattacg gttcaaaat ggaaaacct acgatcact 
tgaataagt cacacggac gtattttta aagcttgta 
aggcttgtc ttgaagttt ttgacaatg aaaatcaat 
tacaagccc actatgata gtgtacatg gtgggggta 
aaaacaata taccggtcg taacgtcac aagttagcc 
aaccacaaa atcactgtg agagtgaaa gtgagcgag 
aaacgaata gcgaacaaa gtgatgagc aagaagaag 
acgaacaat acgataata aaatctcac tattggaga 
aacgtctac ttgggaaaa atgagaagg tgttaccaa 
ccaaaatta ataataaaa aactcaaag aactcgatt 
ttgacacga atccgcaac ataacagtt caaaacgta 
cagtttacg tcaatagta acgatccgt cattttgcg 
agatatgtc acaagacat ggcctgcta agaaacatc 
tctggcaac actatatcc ggtgtcata aatcagtgg 
cggctacgg tttgtggca tttgtctca aaacaaatt 
cctcctatg gtgcacttc aagcccact tgatggaaa 
ctcatccaa cgcttttgc acggtcaag gtttcggtt 
cgaatttta tttgtcgta tttgtggtc gttatcaca 
atgcgtcaa gtaaattaa aattgcata tggcgattc 
atgagaatt ttgattggt cgatgcttg aacaaccga 
aagaggaga agaaggaag agaatgggt tccaggatg 
cgtctgaaa ttaattacg agagtttga ttgaacaag 
tcgagtgtt cctcgggtt cggaaagtg aaaatgtcg 
aaacgaaag ataaggaat caagttcgg aggatgata 
gtgatagtg agacgaacg aagcgagtt ctgtgagtg 
aggaagaag agtcgagtg gaagttggt ttatggtta 
aaagtgaag agagtgagg aggaaaagt tggaatcgg 
cgatcagat cgattaaat atgagtgcg tggaaaagg 
tagaggagg aggtggagg aggaatatt gcgatatgc 
aagcgcaaa gtttgaatg ataatgaaa tcgagtcgg 
cggtgggga gcatattgt aaataagga taggttaga 
attcgatgt tcagttttt atgatgcat atctgtata 
taactcatg atttttttt tctgatgta atatgaaga 
gactgtttt tttttaata atgagtatt taggattgt 
aagtgatct gcttgttta ggataggat attctaagc 
tattttttt atatttatt tttttatat tgatttcga 
ctagtacat aacttaagt cattcccat actcagtaa 
ttgtaaggc tatgtgtat ataaaatga tattcatga 
agtaataca taattttct atgatgcgc tctatttta 
ttcatgtca gtgggacca ccggacgtc cggatgtgg 
taagtcaat ggtttggtt tgccaaatt ttgcctcaa 
aatcattaa taaatcggt taaaacatg