Status
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Gene information
General information
TC015347 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 123269..127824
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015347
    iBeetle screen iB_02467

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015347 RA
      METKLCRIC GKLGQNYVN IFRTEGLKN KIETCLPII 
      VSPHSLLPD TICGECSDN VDNFYSFIK NCLQNIIIL 
      EAQYDINES CLKTKRKHD KGCTTDLAR KSDKNTQTI 
      DYLDLLLKE NELNFPNLK KLVDYDDSD SEQDVKKAA 
      SIFDNFGRK YFDDAENYL IDEITQRKK RKNEDTIES 
      SRAKICKIE TTNRRKNKQ PKKLEIKND FLGDLPQVC 
      LLCDEQFTG PSMLASHVF ETHGIDMAA VTGDTNDKK 
      KKLPNLLKI SDLRKSDSI EEEEPPTLL PSFVCSLCP 
      AMFTSRPDL FGHLRVKHS DQSALLCGM CLYQASSLA 
      NLQSHVELC SHQHPTTTK YVCQVCRYG DDNSKSLEN 
      HILVHDFLL DACKKQSKM FDPADYIET NGNYESGGS 
      SPMTKSLAC SECGMDDFD NFKEFSTHR RSQHSIFHC 
      DLCNKFYGR NSHLWKHVN RLHKGHPSI TCQLCYKTS 
      ASKYHLAQH FNKIHSTKS CKKSENQLN FDNTDDFMS 
      QKFQAFDFQ SVKQSFMRQ ELLEQQEKK FSENDDNSN 
      NHLSDQEPP SAPKEIDSS SDLYTNIIT NYTPPANEG 
      EFKCPKCGK GFHKKTLLK KHKKNCRPR LQKDLLTRC 
      KSCARIFKD RQSLAKHLV NYHSEYACE ICGQKVQSK 
      CEIVSHIRF THPGCHLFC TICGNILRS RNDMADHTR 
      DHMNSYVCQ FCADALPSK IKLKMHILS LHRKILSLS 
      CGVCLKLFE TQHVLRDHV RLVHRDQLA PLTSCPVCG 
      KNYGSKWKT YDHLNKSHG RIFKACKAC LEVFDNENQ 
      LQAHYDSVH GGGKNNIPV VTSQVSQPQ NHCESESER 
      ETNSEQSDE QEEDEQYDN KISLLEKRL LGKNEKVLP 
      TKINNKKLK ELDFDTNPQ HNSSKRTVY VNSNDPSFC 
      EICHKTWPA KKHLWQHYI RCHKSVAAT VCGICLKTN 
      SSYGALQAH LMETHPTLL HGQGFGSNF ICRICGRYH 
      NASSKLKLH MAIHENFDW SMLEQPKEE KKEENGFQD 
      ASEINYESL IEQVECSSG SESENVETK DKESSSEDD 
      SDSETNEAS SVSEEEESS GSWFMVKSE ESEEEKLES 
      AIRSIKYEC VEKVEEEVE EEYCDMQAQ SLNDNEIES 
      AVGSIL
    CDS sequence
    • >TC015347 RA
      atggagacg aaactgtgc cgaatatgc ggaaaactc 
      ggccaaaac tacgtgaat atttttcga accgaaggc 
      ctgaagaac aagatcgaa acatgtctc cctattatt 
      gtgtcgccc cattcactt ctccccgat acgatttgt 
      ggcgaatgc tcagacaat gtggacaat ttctacagt 
      tttatcaag aattgtctc cagaatatt atcatactt 
      gaagcccaa tatgacata aacgagtct tgtttgaag 
      acaaaacgc aaacatgac aaaggatgc accactgat 
      ttggccaga aaaagcgac aaaaatact caaactatc 
      gattatctt gatctcctc ctcaaagaa aacgaattg 
      aattttcct aaccttaaa aaactggtc gactatgac 
      gactctgat tctgaacaa gacgtcaaa aaagcagcg 
      tcgatcttt gataatttt ggccgaaaa tatttcgac 
      gatgcagaa aactacctt atcgacgaa attactcaa 
      cgtaaaaag cgcaaaaac gaagataca atcgaatcg 
      tcacgtgca aaaatctgt aaaatcgaa acaaccaat 
      cggcggaaa aacaaacaa ccgaaaaaa ctcgaaatc 
      aagaacgat tttctagga gatttaccg caagtttgt 
      ttattatgc gatgaacaa ttcactggt ccttccatg 
      ttggctagt cacgtgttt gaaacgcac ggcattgac 
      atggcggcc gttaccggc gatactaac gacaaaaag 
      aaaaagtta ccaaatcta ctcaagatt tcagatctc 
      aggaaaagc gattcgatc gaagaagag gagccgccg 
      actcttctc ccgagcttc gtttgttcc ctttgtccg 
      gcgatgttt acatcacgt ccggacttg tttggccat 
      cttcgtgtg aaacattcc gaccaatcg gctctctta 
      tgtggcatg tgtctctat caagcttca tcattggct 
      aatcttcag tcacatgtt gaattatgt tcccaccaa 
      catccaacc acaacaaaa tatgtctgt caggtgtgt 
      cgttatggt gatgataat tccaaatca ttggaaaat 
      catatactc gtccatgac tttttgttg gatgcgtgt 
      aaaaaacaa tcgaaaatg ttcgatcca gccgactat 
      atcgaaacg aatggtaat tacgaatcg ggtggttcg 
      tcaccaatg acaaaaagt ctagcgtgt agtgagtgc 
      ggaatggac gattttgat aattttaaa gaatttagt 
      acgcataga agaagccaa cattcaatt tttcattgc 
      gatctttgt aataaattt tacggccgt aattcgcat 
      ttatggaaa catgtcaat cgattgcat aaaggacat 
      ccaagtatc acgtgtcag ttgtgctac aaaacgagt 
      gcttcaaag tatcatctc gcacaacat ttcaataaa 
      atccacagt acgaagagc tgcaaaaag agcgaaaat 
      caattgaat tttgacaat acggacgat tttatgtca 
      cagaaattc caagctttt gattttcaa tcggtcaaa 
      cagagtttc atgcgacag gaattactc gaacaacag 
      gaaaagaag ttttccgaa aacgacgac aactcgaat 
      aatcattta tccgatcag gaaccacca tctgcacct 
      aaagaaatt gactcatct agtgacctc tacactaac 
      attattaca aactatacg ccacctgcc aacgaaggc 
      gaattcaaa tgtcccaag tgtggtaaa ggttttcac 
      aagaaaacc ctccttaag aaacacaaa aagaactgt 
      agaccacgt ctccagaaa gatttactc actcgttgc 
      aaatcatgt gctcgtata tttaaagat cgccaaagt 
      ctcgccaaa catttggtt aattaccat tcagagtat 
      gcgtgtgaa atatgcggt caaaaagtc caaagtaag 
      tgtgagatt gtctcacat attaggttc acacatccc 
      ggttgtcat ttgttttgt acgatatgt ggtaacata 
      ttacgtagt cgtaacgat atggccgac cacacacgt 
      gaccatatg aatagttac gtctgtcaa ttctgcgct 
      gatgcgtta ccaagcaaa atcaaactt aaaatgcac 
      attttaagt ctacatcgt aaaatatta agtttaagt 
      tgcggtgtt tgtttgaaa ttgtttgaa acgcaacat 
      gttctacgc gaccatgtc aggttagta catcgtgat 
      cagttggcg ccactgact tcgtgtccc gtctgtggt 
      aaaaattac ggttcaaaa tggaaaacc tacgatcac 
      ttgaataag tcacacgga cgtattttt aaagcttgt 
      aaggcttgt cttgaagtt tttgacaat gaaaatcaa 
      ttacaagcc cactatgat agtgtacat ggtgggggt 
      aaaaacaat ataccggtc gtaacgtca caagttagc 
      caaccacaa aatcactgt gagagtgaa agtgagcga 
      gaaacgaat agcgaacaa agtgatgag caagaagaa 
      gacgaacaa tacgataat aaaatctca ctattggag 
      aaacgtcta cttgggaaa aatgagaag gtgttacca 
      accaaaatt aataataaa aaactcaaa gaactcgat 
      tttgacacg aatccgcaa cataacagt tcaaaacgt 
      acagtttac gtcaatagt aacgatccg tcattttgc 
      gagatatgt cacaagaca tggcctgct aagaaacat 
      ctctggcaa cactatatc cggtgtcat aaatcagtg 
      gcggctacg gtttgtggc atttgtctc aaaacaaat 
      tcctcctat ggtgcactt caagcccac ttgatggaa 
      actcatcca acgcttttg cacggtcaa ggtttcggt 
      tcgaatttt atttgtcgt atttgtggt cgttatcac 
      aatgcgtca agtaaatta aaattgcat atggcgatt 
      catgagaat tttgattgg tcgatgctt gaacaaccg 
      aaagaggag aagaaggaa gagaatggg ttccaggat 
      gcgtctgaa attaattac gagagtttg attgaacaa 
      gtcgagtgt tcctcgggt tcggaaagt gaaaatgtc 
      gaaacgaaa gataaggaa tcaagttcg gaggatgat 
      agtgatagt gagacgaac gaagcgagt tctgtgagt 
      gaggaagaa gagtcgagt ggaagttgg tttatggtt 
      aaaagtgaa gagagtgag gaggaaaag ttggaatcg 
      gcgatcaga tcgattaaa tatgagtgc gtggaaaag 
      gtagaggag gaggtggag gaggaatat tgcgatatg 
      caagcgcaa agtttgaat gataatgaa atcgagtcg 
      gcggtgggg agcatattg taa
    mRNA sequence
    • >TC015347 RA
      tcgagtggg ttttgtttg ttggtttta acgtaaaaa 
      ttaattcgg gagtagttt taatacacg gccagtgtt 
      ttagttgtg tttctcgtt ttgttgggg gtggaaaag 
      ccggtgaaa cgccgtcta gtgtgcaaa attgacact 
      aaaatcagc aatggcgtc gttgtgttt gtgctcgaa 
      ttgttgaaa attgcaaat ttcggtgca tcaaagcga 
      tgaatgtat ttttgatgc gttaagagc cccctttgt 
      ctttgaggc tccaggggg ccccgctat gcacgagaa 
      tggagacga aactgtgcc gaatatgcg gaaaactcg 
      gccaaaact acgtgaata tttttcgaa ccgaaggcc 
      tgaagaaca agatcgaaa catgtctcc ctattattg 
      tgtcgcccc attcacttc tccccgata cgatttgtg 
      gcgaatgct cagacaatg tggacaatt tctacagtt 
      ttatcaaga attgtctcc agaatatta tcatacttg 
      aagcccaat atgacataa acgagtctt gtttgaaga 
      caaaacgca aacatgaca aaggatgca ccactgatt 
      tggccagaa aaagcgaca aaaatactc aaactatcg 
      attatcttg atctcctcc tcaaagaaa acgaattga 
      attttccta accttaaaa aactggtcg actatgacg 
      actctgatt ctgaacaag acgtcaaaa aagcagcgt 
      cgatctttg ataattttg gccgaaaat atttcgacg 
      atgcagaaa actacctta tcgacgaaa ttactcaac 
      gtaaaaagc gcaaaaacg aagatacaa tcgaatcgt 
      cacgtgcaa aaatctgta aaatcgaaa caaccaatc 
      ggcggaaaa acaaacaac cgaaaaaac tcgaaatca 
      agaacgatt ttctaggag atttaccgc aagtttgtt 
      tattatgcg atgaacaat tcactggtc cttccatgt 
      tggctagtc acgtgtttg aaacgcacg gcattgaca 
      tggcggccg ttaccggcg atactaacg acaaaaaga 
      aaaagttac caaatctac tcaagattt cagatctca 
      ggaaaagcg attcgatcg aagaagagg agccgccga 
      ctcttctcc cgagcttcg tttgttccc tttgtccgg 
      cgatgttta catcacgtc cggacttgt ttggccatc 
      ttcgtgtga aacattccg accaatcgg ctctcttat 
      gtggcatgt gtctctatc aagcttcat cattggcta 
      atcttcagt cacatgttg aattatgtt cccaccaac 
      atccaacca caacaaaat atgtctgtc aggtgtgtc 
      gttatggtg atgataatt ccaaatcat tggaaaatc 
      atatactcg tccatgact ttttgttgg atgcgtgta 
      aaaaacaat cgaaaatgt tcgatccag ccgactata 
      tcgaaacga atggtaatt acgaatcgg gtggttcgt 
      caccaatga caaaaagtc tagcgtgta gtgagtgcg 
      gaatggacg attttgata attttaaag aatttagta 
      cgcatagaa gaagccaac attcaattt ttcattgcg 
      atctttgta ataaatttt acggccgta attcgcatt 
      tatggaaac atgtcaatc gattgcata aaggacatc 
      caagtatca cgtgtcagt tgtgctaca aaacgagtg 
      cttcaaagt atcatctcg cacaacatt tcaataaaa 
      tccacagta cgaagagct gcaaaaaga gcgaaaatc 
      aattgaatt ttgacaata cggacgatt ttatgtcac 
      agaaattcc aagcttttg attttcaat cggtcaaac 
      agagtttca tgcgacagg aattactcg aacaacagg 
      aaaagaagt tttccgaaa acgacgaca actcgaata 
      atcatttat ccgatcagg aaccaccat ctgcaccta 
      aagaaattg actcatcta gtgacctct acactaaca 
      ttattacaa actatacgc cacctgcca acgaaggcg 
      aattcaaat gtcccaagt gtggtaaag gttttcaca 
      agaaaaccc tccttaaga aacacaaaa agaactgta 
      gaccacgtc tccagaaag atttactca ctcgttgca 
      aatcatgtg ctcgtatat ttaaagatc gccaaagtc 
      tcgccaaac atttggtta attaccatt cagagtatg 
      cgtgtgaaa tatgcggtc aaaaagtcc aaagtaagt 
      gtgagattg tctcacata ttaggttca cacatcccg 
      gttgtcatt tgttttgta cgatatgtg gtaacatat 
      tacgtagtc gtaacgata tggccgacc acacacgtg 
      accatatga atagttacg tctgtcaat tctgcgctg 
      atgcgttac caagcaaaa tcaaactta aaatgcaca 
      ttttaagtc tacatcgta aaatattaa gtttaagtt 
      gcggtgttt gtttgaaat tgtttgaaa cgcaacatg 
      ttctacgcg accatgtca ggttagtac atcgtgatc 
      agttggcgc cactgactt cgtgtcccg tctgtggta 
      aaaattacg gttcaaaat ggaaaacct acgatcact 
      tgaataagt cacacggac gtattttta aagcttgta 
      aggcttgtc ttgaagttt ttgacaatg aaaatcaat 
      tacaagccc actatgata gtgtacatg gtgggggta 
      aaaacaata taccggtcg taacgtcac aagttagcc 
      aaccacaaa atcactgtg agagtgaaa gtgagcgag 
      aaacgaata gcgaacaaa gtgatgagc aagaagaag 
      acgaacaat acgataata aaatctcac tattggaga 
      aacgtctac ttgggaaaa atgagaagg tgttaccaa 
      ccaaaatta ataataaaa aactcaaag aactcgatt 
      ttgacacga atccgcaac ataacagtt caaaacgta 
      cagtttacg tcaatagta acgatccgt cattttgcg 
      agatatgtc acaagacat ggcctgcta agaaacatc 
      tctggcaac actatatcc ggtgtcata aatcagtgg 
      cggctacgg tttgtggca tttgtctca aaacaaatt 
      cctcctatg gtgcacttc aagcccact tgatggaaa 
      ctcatccaa cgcttttgc acggtcaag gtttcggtt 
      cgaatttta tttgtcgta tttgtggtc gttatcaca 
      atgcgtcaa gtaaattaa aattgcata tggcgattc 
      atgagaatt ttgattggt cgatgcttg aacaaccga 
      aagaggaga agaaggaag agaatgggt tccaggatg 
      cgtctgaaa ttaattacg agagtttga ttgaacaag 
      tcgagtgtt cctcgggtt cggaaagtg aaaatgtcg 
      aaacgaaag ataaggaat caagttcgg aggatgata 
      gtgatagtg agacgaacg aagcgagtt ctgtgagtg 
      aggaagaag agtcgagtg gaagttggt ttatggtta 
      aaagtgaag agagtgagg aggaaaagt tggaatcgg 
      cgatcagat cgattaaat atgagtgcg tggaaaagg 
      tagaggagg aggtggagg aggaatatt gcgatatgc 
      aagcgcaaa gtttgaatg ataatgaaa tcgagtcgg 
      cggtgggga gcatattgt aaataagga taggttaga 
      attcgatgt tcagttttt atgatgcat atctgtata 
      taactcatg atttttttt tctgatgta atatgaaga 
      gactgtttt tttttaata atgagtatt taggattgt 
      aagtgatct gcttgttta ggataggat attctaagc 
      tattttttt atatttatt tttttatat tgatttcga 
      ctagtacat aacttaagt cattcccat actcagtaa 
      ttgtaaggc tatgtgtat ataaaatga tattcatga 
      agtaataca taattttct atgatgcgc tctatttta 
      ttcatgtca gtgggacca ccggacgtc cggatgtgg 
      taagtcaat ggtttggtt tgccaaatt ttgcctcaa 
      aatcattaa taaatcggt taaaacatg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY