Status
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Gene information
General information
TC033309
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC015335
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 276207..280042
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033309
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02463

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature complete fluorescence not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    No observeable cuticle phenotype found.

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033309 RA
      MATEIDQNR DRIAETLVG RTLLITGGT GFVGKVLIE 
      KILRCLDVK KIYVLVRPK KGKSPTERR AALFADPLF 
      ELAKKTRGD DIVKRVEFV SGDIAAPGL ALSTSDRQK 
      LAAECEFIF HCAATIRFD MDLKPAVLL NVRGTKLML 
      ELAKECKKL LNFVHLSTA YCHLNERVL YEKAYPPPA 
      DPHSIIKSC ELMSNEAVN SIAPKLLEE WPNSYAFTK 
      ALGEALVHD QVGKIPLII LRPSIIMPI LKEPIPGWT 
      DNINGPMGL LIAAGKGVL RTMYGRSEA YADYVPVDI 
      VVNVMIGSA CDRLVLKSS ERIFYNITS SAEYKLTFQ 
      EMLDIGRDT VYNRIPLNG VFWYPGGSM KRSRLAHNL 
      AFFFYQWVP AVIVDILLV CLGYKPVLK RVQRRILKG 
      YDVFEYYAN RQWDFDNEG SFKARKLMT EKERQLYKV 
      DGDGISYED YFYNCVKSA RLYILNETD DTIPAAKRH 
      MKVMYCVDR ICKSLIIFG LMYLVWRFF LIPIFGF
    CDS sequence
    • >TC033309 RA
      atggcaacg gaaatcgac cagaaccgt gaccgcatt 
      gccgaaacc ctcgtcggt cgtacctta cttataaca 
      ggtggtact ggttttgtg ggcaaagta ctcattgaa 
      aagatacta cgctgtctc gatgtgaag aaaatctac 
      gttctggta cgacctaaa aaaggcaaa tctccgacg 
      gaacgacga gctgcactt tttgccgat ccgcttttc 
      gagttggcc aaaaaaaca cgtggcgat gacatcgtc 
      aagcgggtg gagtttgtg tcgggggac attgccgcc 
      cccggcctg gccttgagt accagcgac cgccaaaaa 
      ttggcggcg gagtgtgag tttattttt cattgcgcc 
      gccaccatt cgttttgat atggatttg aaacctgcg 
      gtactactt aatgtcaga ggtaccaaa cttatgctc 
      gagctggca aaagagtgc aaaaaactt ttgaatttt 
      gtccatctt tctacggct tattgtcac ttgaacgag 
      agagtgctt tacgagaag gcgtacccg cccccagct 
      gaccctcat agcatcata aaatcatgc gaattgatg 
      agcaacgaa gctgtaaac agtattgcc ccaaaactt 
      ttggaagag tggcccaac agttacgcc ttcaccaag 
      gctttgggc gaggcttta gtccacgat caagtcggc 
      aaaatccct ctcattatt ttacgacct tccataatt 
      atgccaatt ttgaaagaa cccatccct gggtggacc 
      gacaacatt aatggtccc atgggactt ttgatagcc 
      gccggaaaa ggggtactt cgtaccatg tatgggcga 
      agcgaagcc tacgctgat tacgtccca gttgatatc 
      gtagtcaac gtcatgatc ggaagtgct tgtgatcgt 
      ctcgtgctc aaatcttcg gaacgcatc ttttataac 
      ataacgagc tcggctgaa tataagcta accttccaa 
      gaaatgttg gacattggt cgtgatact gtctataac 
      cgaattccc ttaaacggg gtgttttgg taccctggt 
      ggttccatg aaacgttca cgtctcgcc cacaactta 
      gccttcttt ttttaccaa tgggtaccg gcagttatc 
      gttgatata ctccttgta tgtcttggg tataaaccg 
      gtactcaaa cgggtacaa cgaaggatc cttaaaggg 
      tacgacgtt ttcgagtac tatgctaac agacagtgg 
      gattttgac aatgaaggc tcgttcaaa gcccggaaa 
      ttgatgaca gaaaaagaa cgacagttg tacaaggtt 
      gatggagat gggattagt tacgaggat tatttctac 
      aattgtgtc aaatcggcc aggttgtat attttaaac 
      gagacggat gatacgatt ccggcggct aaaaggcac 
      atgaaagtg atgtactgt gtggatagg atttgcaag 
      tcgttgatt atttttggc ttgatgtat ctggtttgg 
      agatttttt cttatccca atttttgga ttctag
    mRNA sequence
    • >TC033309 RA
      taatagtac ctaagttga ggcagtttg gttagtttc 
      gactcagac tgcgttaga cgagttcaa aaaattatt 
      agttgctat tttttcgat tttttgaga gagtagtac 
      cactacaga tggcaacgg aaatcgacc agaaccgtg 
      accgcattg ccgaaaccc tcgtcggtc gtaccttac 
      ttataacag gtggtactg gttttgtgg gcaaagtac 
      tcattgaaa agatactac gctgtctcg atgtgaaga 
      aaatctacg ttctggtac gacctaaaa aaggcaaat 
      ctccgacgg aacgacgag ctgcacttt ttgccgatc 
      cgcttttcg agttggcca aaaaaacac gtggcgatg 
      acatcgtca agcgggtgg agtttgtgt cgggggaca 
      ttgccgccc ccggcctgg ccttgagta ccagcgacc 
      gccaaaaat tggcggcgg agtgtgagt ttatttttc 
      attgcgccg ccaccattc gttttgata tggatttga 
      aacctgcgg tactactta atgtcagag gtaccaaac 
      ttatgctcg agctggcaa aagagtgca aaaaacttt 
      tgaattttg tccatcttt ctacggctt attgtcact 
      tgaacgaga gagtgcttt acgagaagg cgtacccgc 
      ccccagctg accctcata gcatcataa aatcatgcg 
      aattgatga gcaacgaag ctgtaaaca gtattgccc 
      caaaacttt tggaagagt ggcccaaca gttacgcct 
      tcaccaagg ctttgggcg aggctttag tccacgatc 
      aagtcggca aaatccctc tcattattt tacgacctt 
      ccataatta tgccaattt tgaaagaac ccatccctg 
      ggtggaccg acaacatta atggtccca tgggacttt 
      tgatagccg ccggaaaag gggtacttc gtaccatgt 
      atgggcgaa gcgaagcct acgctgatt acgtcccag 
      ttgatatcg tagtcaacg tcatgatcg gaagtgctt 
      gtgatcgtc tcgtgctca aatcttcgg aacgcatct 
      tttataaca taacgagct cggctgaat ataagctaa 
      ccttccaag aaatgttgg acattggtc gtgatactg 
      tctataacc gaattccct taaacgggg tgttttggt 
      accctggtg gttccatga aacgttcac gtctcgccc 
      acaacttag ccttctttt tttaccaat gggtaccgg 
      cagttatcg ttgatatac tccttgtat gtcttgggt 
      ataaaccgg tactcaaac gggtacaac gaaggatcc 
      ttaaagggt acgacgttt tcgagtact atgctaaca 
      gacagtggg attttgaca atgaaggct cgttcaaag 
      cccggaaat tgatgacag aaaaagaac gacagttgt 
      acaaggttg atggagatg ggattagtt acgaggatt 
      atttctaca attgtgtca aatcggcca ggttgtata 
      ttttaaacg agacggatg atacgattc cggcggcta 
      aaaggcaca tgaaagtga tgtactgtg tggatagga 
      tttgcaagt cgttgatta tttttggct tgatgtatc 
      tggtttgga gattttttc ttatcccaa tttttggat 
      tctagtacc aacaagtac cacttagca aattggtga 
      ttgtgatta gtattttgt aaatagtgc acaaaaaga 
      cgaccaaat acagttttt tatatttg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY