Status
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Gene information
General information
TC015333 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 332575..337542
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015333
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02462

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015333 RA
      MPTVVSKFL SKGEAALNV NRRALSGAV VAAALFAYT 
      YKIAYPLLT RKTDVNINN NLVRRNIQN GLKSNKLPK 
      RHRLRNTIP ALNLQFILQ FIKLARIMI PSVFCAEIG 
      LLGGHTTFL FLRTFLSIY VANLEGAIV KYIVRKEPK 
      NFIKQLVKW FAVAIPATF INSMIKYLE SKIALSFRT 
      RLVEHSYQL YFRNQSYYR VTVLDGRLD NCAQRLTDD 
      IETVASTVS HLYGQITKP MFDILLMAI ALANLVKSR 
      NANLITGPA IISGVVIFS ALILRLVSP RFGQLVAQE 
      AEKKGYLRH VHSRIVSNA EEIAFYGGH KVEQSQLRQ 
      AFRILAQHM EHMFGVKLW FVMLEQFLM KYVWSGTGM 
      IVVSLPILL SSGPGKAPT EGEISDNVA ERTHYFTTA 
      KNLLITGSN AVERLMSSY KDIVELAGH TARVANMFT 
      VLEEASQGI YHKTLVEKK EKCGDFEIE FKGDQPLAK 
      GKLIVSTTN EIILRNVPI VTPNCDVVC PSLSLELKP 
      GQHLLITGP NGCGKSSLF RILSGLWPI YGGELHTPK 
      NSMFYIPQR PYMVIGNLR DQIIYPDTA TDMINKQVS 
      EDELRKIMR LVHLEHIVE RDTFHQVKD WTDILSGGE 
      KQRMAIARL FYHKPKYAL LDECTSAVS IDVESFIYQ 
      TAIDMGITL LTITHRPTL WKFHTHILQ FDGTGAWKF 
      EELNANNRL SLKKEKEEL MKKKSTEET IQRLNELNK 
      LLGEDD
    CDS sequence
    • >TC015333 RA
      atgccaacg gtcgtgtca aaattcttg tccaagggc 
      gaggcagcc ctgaatgtg aaccgaagg gcgctttcg 
      ggggccgtg gtggccgcg gcccttttt gcctacacg 
      tacaaaatc gcctatcct cttctaaca cgtaaaacg 
      gacgtaaac atcaacaat aatctagtc agaagaaat 
      atccaaaat gggctcaaa agcaacaaa ttgcccaaa 
      agacaccgc ttacgtaat accatcccg gccctgaat 
      ctacaattt attttgcaa tttatcaag ttggcgagg 
      attatgatt ccgagtgtt ttttgtgcc gagattggc 
      cttctggga gggcacacg acgtttttg tttttgcgg 
      acgtttttg agcatttat gtggcgaac ttggagggg 
      gccattgtc aaatatatc gtaagaaaa gagccgaaa 
      aattttatc aaacagttg gttaagtgg tttgctgtt 
      gcaattccg gccactttt atcaacagc atgatcaaa 
      tatttggag agcaagatt gcgctgagc ttcaggacg 
      cgattagtg gaacattcg taccaacta tacttcagg 
      aaccaaagt tattacagg gtcaccgtc ctggacgga 
      cgcctggat aattgcgcc cagcgtctc accgacgac 
      attgagacc gtggccagc accgtttcg cacctttac 
      ggccagatc accaagccc atgttcgac atcctcctc 
      atggccatc gcgttggcc aacttggtc aaaagtcgc 
      aacgccaac ttgatcaca ggccccgcc atcatctca 
      ggtgttgtg attttctca gcgttgatt ttacggttg 
      gtttcgcca cgttttggc caacttgtg gcccaggag 
      gctgagaaa aagggttat ttacgtcat gtgcactct 
      cgaattgtt tcaaacgcc gaagaaatc gccttttac 
      ggcggtcat aaagtcgaa caatcgcaa ctgcgccaa 
      gccttccgc attttggcg caacatatg gagcacatg 
      ttcggcgta aaattatgg tttgtgatg ttggagcag 
      tttttaatg aaatatgtg tggtctggc acggggatg 
      attgtggtt tcgttgccg attttatta tctagtggg 
      cctgggaaa gcccctact gagggcgaa atttccgat 
      aatgtggcc gaaaggacg cattacttc accacggca 
      aaaaatcta ctgattacg ggttcaaat gcagttgag 
      agattaatg tcaagttat aaagatatc gtcgagttg 
      gcgggacat acggccagg gtggccaac atgttcacg 
      gttttggag gaggcgagt caagggatt tatcacaag 
      accttggtt gagaagaag gagaagtgt ggcgacttt 
      gaaattgaa tttaaaggc gaccaacca ctcgctaaa 
      ggcaaatta atcgtctcg accaccaat gaaatcatc 
      ctccgtaac gtccccata gtcaccccc aattgtgac 
      gtggtgtgc ccctcctta agcctcgaa ttaaagccg 
      ggccaacac ttgctcatt acgggccct aatggctgc 
      ggaaaatcc agccttttc cgcattcta agtgggctg 
      tggcccatc tatggaggc gaactccac acccccaag 
      aactcaatg ttctacatc ccccagcgg ccatacatg 
      gtcataggc aacttgaga gaccagatc atttatccg 
      gacacagcc accgacatg atcaataaa caagtgagc 
      gaggatgag ctccggaaa atcatgcga ttggtgcac 
      ttggagcac atcgtcgag agggacacc ttccaccag 
      gtcaaggat tggacggat attttgtca ggcggcgaa 
      aagcagcga atggcaatc gcgcgattg ttttaccac 
      aagccgaag tacgcgctg ttggacgag tgcacgtcg 
      gcggtgtcc atagatgtt gaaagcttc atttatcaa 
      acggccatt gacatggga atcacactt ttgacaatt 
      acgcatcgg cccactctt tggaaattt cacactcat 
      attttgcaa tttgacggc acaggtgcg tggaagttt 
      gaagaattg aatgccaat aataggctc agtttgaag 
      aaggagaaa gaggaatta atgaagaag aaaagtact 
      gaagaaacc attcaaaga ctcaatgaa ctgaacaaa 
      cttttaggc gaagatgac tag
    mRNA sequence
    • >TC015333 RA
      atctgtcac tttcgctct ttagtcaag cggcgatcg 
      tcatttgtt gttgtaacc attggaaat atgtaacaa 
      gtttgtaag caaaaaact agtgtgctg tggctcttt 
      gtgtaacat caagatagc cgtgacaac gcttccggt 
      tttttatca caggaaaat gccaacttg attggtgaa 
      aaatgccaa cggtcgtgt caaaattct tgtccaagg 
      gcgaggcag ccctgaatg tgaaccgaa gggcgcttt 
      cgggggccg tggtggccg cggcccttt ttgcctaca 
      cgtacaaaa tcgcctatc ctcttctaa cacgtaaaa 
      cggacgtaa acatcaaca ataatctag tcagaagaa 
      atatccaaa atgggctca aaagcaaca aattgccca 
      aaagacacc gcttacgta ataccatcc cggccctga 
      atctacaat ttattttgc aatttatca agttggcga 
      ggattatga ttccgagtg ttttttgtg ccgagattg 
      gccttctgg gagggcaca cgacgtttt tgtttttgc 
      ggacgtttt tgagcattt atgtggcga acttggagg 
      gggccattg tcaaatata tcgtaagaa aagagccga 
      aaaatttta tcaaacagt tggttaagt ggtttgctg 
      ttgcaattc cggccactt ttatcaaca gcatgatca 
      aatatttgg agagcaaga ttgcgctga gcttcagga 
      cgcgattag tggaacatt cgtaccaac tatacttca 
      ggaaccaaa gttattaca gggtcaccg tcctggacg 
      gacgcctgg ataattgcg cccagcgtc tcaccgacg 
      acattgaga ccgtggcca gcaccgttt cgcaccttt 
      acggccaga tcaccaagc ccatgttcg acatcctcc 
      tcatggcca tcgcgttgg ccaacttgg tcaaaagtc 
      gcaacgcca acttgatca caggccccg ccatcatct 
      caggtgttg tgattttct cagcgttga ttttacggt 
      tggtttcgc cacgttttg gccaacttg tggcccagg 
      aggctgaga aaaagggtt atttacgtc atgtgcact 
      ctcgaattg tttcaaacg ccgaagaaa tcgcctttt 
      acggcggtc ataaagtcg aacaatcgc aactgcgcc 
      aagccttcc gcattttgg cgcaacata tggagcaca 
      tgttcggcg taaaattat ggtttgtga tgttggagc 
      agtttttaa tgaaatatg tgtggtctg gcacgggga 
      tgattgtgg tttcgttgc cgattttat tatctagtg 
      ggcctggga aagccccta ctgagggcg aaatttccg 
      ataatgtgg ccgaaagga cgcattact tcaccacgg 
      caaaaaatc tactgatta cgggttcaa atgcagttg 
      agagattaa tgtcaagtt ataaagata tcgtcgagt 
      tggcgggac atacggcca gggtggcca acatgttca 
      cggttttgg aggaggcga gtcaaggga tttatcaca 
      agaccttgg ttgagaaga aggagaagt gtggcgact 
      ttgaaattg aatttaaag gcgaccaac cactcgcta 
      aaggcaaat taatcgtct cgaccacca atgaaatca 
      tcctccgta acgtcccca tagtcaccc ccaattgtg 
      acgtggtgt gcccctcct taagcctcg aattaaagc 
      cgggccaac acttgctca ttacgggcc ctaatggct 
      gcggaaaat ccagccttt tccgcattc taagtgggc 
      tgtggccca tctatggag gcgaactcc acaccccca 
      agaactcaa tgttctaca tcccccagc ggccataca 
      tggtcatag gcaacttga gagaccaga tcatttatc 
      cggacacag ccaccgaca tgatcaata aacaagtga 
      gcgaggatg agctccgga aaatcatgc gattggtgc 
      acttggagc acatcgtcg agagggaca ccttccacc 
      aggtcaagg attggacgg atattttgt caggcggcg 
      aaaagcagc gaatggcaa tcgcgcgat tgttttacc 
      acaagccga agtacgcgc tgttggacg agtgcacgt 
      cggcggtgt ccatagatg ttgaaagct tcatttatc 
      aaacggcca ttgacatgg gaatcacac ttttgacaa 
      ttacgcatc ggcccactc tttggaaat ttcacactc 
      atattttgc aatttgacg gcacaggtg cgtggaagt 
      ttgaagaat tgaatgcca ataataggc tcagtttga 
      agaaggaga aagaggaat taatgaaga agaaaagta 
      ctgaagaaa ccattcaaa gactcaatg aactgaaca 
      aacttttag gcgaagatg actagatcg tgtagttca 
      atagctcta gctggtgaa ttgacagag atatcaatt 
      aaagttgat ttatttat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY