| General information | |||
| TC015333 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007421.3: 332575..337542 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC015333 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02462 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
- >TC015333 RA
MPTVVSKFL SKGEAALNV NRRALSGAV VAAALFAYT YKIAYPLLT RKTDVNINN NLVRRNIQN GLKSNKLPK RHRLRNTIP ALNLQFILQ FIKLARIMI PSVFCAEIG LLGGHTTFL FLRTFLSIY VANLEGAIV KYIVRKEPK NFIKQLVKW FAVAIPATF INSMIKYLE SKIALSFRT RLVEHSYQL YFRNQSYYR VTVLDGRLD NCAQRLTDD IETVASTVS HLYGQITKP MFDILLMAI ALANLVKSR NANLITGPA IISGVVIFS ALILRLVSP RFGQLVAQE AEKKGYLRH VHSRIVSNA EEIAFYGGH KVEQSQLRQ AFRILAQHM EHMFGVKLW FVMLEQFLM KYVWSGTGM IVVSLPILL SSGPGKAPT EGEISDNVA ERTHYFTTA KNLLITGSN AVERLMSSY KDIVELAGH TARVANMFT VLEEASQGI YHKTLVEKK EKCGDFEIE FKGDQPLAK GKLIVSTTN EIILRNVPI VTPNCDVVC PSLSLELKP GQHLLITGP NGCGKSSLF RILSGLWPI YGGELHTPK NSMFYIPQR PYMVIGNLR DQIIYPDTA TDMINKQVS EDELRKIMR LVHLEHIVE RDTFHQVKD WTDILSGGE KQRMAIARL FYHKPKYAL LDECTSAVS IDVESFIYQ TAIDMGITL LTITHRPTL WKFHTHILQ FDGTGAWKF EELNANNRL SLKKEKEEL MKKKSTEET IQRLNELNK LLGEDD
- >TC015333 RA
atgccaacg gtcgtgtca aaattcttg tccaagggc gaggcagcc ctgaatgtg aaccgaagg gcgctttcg ggggccgtg gtggccgcg gcccttttt gcctacacg tacaaaatc gcctatcct cttctaaca cgtaaaacg gacgtaaac atcaacaat aatctagtc agaagaaat atccaaaat gggctcaaa agcaacaaa ttgcccaaa agacaccgc ttacgtaat accatcccg gccctgaat ctacaattt attttgcaa tttatcaag ttggcgagg attatgatt ccgagtgtt ttttgtgcc gagattggc cttctggga gggcacacg acgtttttg tttttgcgg acgtttttg agcatttat gtggcgaac ttggagggg gccattgtc aaatatatc gtaagaaaa gagccgaaa aattttatc aaacagttg gttaagtgg tttgctgtt gcaattccg gccactttt atcaacagc atgatcaaa tatttggag agcaagatt gcgctgagc ttcaggacg cgattagtg gaacattcg taccaacta tacttcagg aaccaaagt tattacagg gtcaccgtc ctggacgga cgcctggat aattgcgcc cagcgtctc accgacgac attgagacc gtggccagc accgtttcg cacctttac ggccagatc accaagccc atgttcgac atcctcctc atggccatc gcgttggcc aacttggtc aaaagtcgc aacgccaac ttgatcaca ggccccgcc atcatctca ggtgttgtg attttctca gcgttgatt ttacggttg gtttcgcca cgttttggc caacttgtg gcccaggag gctgagaaa aagggttat ttacgtcat gtgcactct cgaattgtt tcaaacgcc gaagaaatc gccttttac ggcggtcat aaagtcgaa caatcgcaa ctgcgccaa gccttccgc attttggcg caacatatg gagcacatg ttcggcgta aaattatgg tttgtgatg ttggagcag tttttaatg aaatatgtg tggtctggc acggggatg attgtggtt tcgttgccg attttatta tctagtggg cctgggaaa gcccctact gagggcgaa atttccgat aatgtggcc gaaaggacg cattacttc accacggca aaaaatcta ctgattacg ggttcaaat gcagttgag agattaatg tcaagttat aaagatatc gtcgagttg gcgggacat acggccagg gtggccaac atgttcacg gttttggag gaggcgagt caagggatt tatcacaag accttggtt gagaagaag gagaagtgt ggcgacttt gaaattgaa tttaaaggc gaccaacca ctcgctaaa ggcaaatta atcgtctcg accaccaat gaaatcatc ctccgtaac gtccccata gtcaccccc aattgtgac gtggtgtgc ccctcctta agcctcgaa ttaaagccg ggccaacac ttgctcatt acgggccct aatggctgc ggaaaatcc agccttttc cgcattcta agtgggctg tggcccatc tatggaggc gaactccac acccccaag aactcaatg ttctacatc ccccagcgg ccatacatg gtcataggc aacttgaga gaccagatc atttatccg gacacagcc accgacatg atcaataaa caagtgagc gaggatgag ctccggaaa atcatgcga ttggtgcac ttggagcac atcgtcgag agggacacc ttccaccag gtcaaggat tggacggat attttgtca ggcggcgaa aagcagcga atggcaatc gcgcgattg ttttaccac aagccgaag tacgcgctg ttggacgag tgcacgtcg gcggtgtcc atagatgtt gaaagcttc atttatcaa acggccatt gacatggga atcacactt ttgacaatt acgcatcgg cccactctt tggaaattt cacactcat attttgcaa tttgacggc acaggtgcg tggaagttt gaagaattg aatgccaat aataggctc agtttgaag aaggagaaa gaggaatta atgaagaag aaaagtact gaagaaacc attcaaaga ctcaatgaa ctgaacaaa cttttaggc gaagatgac tag
- >TC015333 RA
atctgtcac tttcgctct ttagtcaag cggcgatcg tcatttgtt gttgtaacc attggaaat atgtaacaa gtttgtaag caaaaaact agtgtgctg tggctcttt gtgtaacat caagatagc cgtgacaac gcttccggt tttttatca caggaaaat gccaacttg attggtgaa aaatgccaa cggtcgtgt caaaattct tgtccaagg gcgaggcag ccctgaatg tgaaccgaa gggcgcttt cgggggccg tggtggccg cggcccttt ttgcctaca cgtacaaaa tcgcctatc ctcttctaa cacgtaaaa cggacgtaa acatcaaca ataatctag tcagaagaa atatccaaa atgggctca aaagcaaca aattgccca aaagacacc gcttacgta ataccatcc cggccctga atctacaat ttattttgc aatttatca agttggcga ggattatga ttccgagtg ttttttgtg ccgagattg gccttctgg gagggcaca cgacgtttt tgtttttgc ggacgtttt tgagcattt atgtggcga acttggagg gggccattg tcaaatata tcgtaagaa aagagccga aaaatttta tcaaacagt tggttaagt ggtttgctg ttgcaattc cggccactt ttatcaaca gcatgatca aatatttgg agagcaaga ttgcgctga gcttcagga cgcgattag tggaacatt cgtaccaac tatacttca ggaaccaaa gttattaca gggtcaccg tcctggacg gacgcctgg ataattgcg cccagcgtc tcaccgacg acattgaga ccgtggcca gcaccgttt cgcaccttt acggccaga tcaccaagc ccatgttcg acatcctcc tcatggcca tcgcgttgg ccaacttgg tcaaaagtc gcaacgcca acttgatca caggccccg ccatcatct caggtgttg tgattttct cagcgttga ttttacggt tggtttcgc cacgttttg gccaacttg tggcccagg aggctgaga aaaagggtt atttacgtc atgtgcact ctcgaattg tttcaaacg ccgaagaaa tcgcctttt acggcggtc ataaagtcg aacaatcgc aactgcgcc aagccttcc gcattttgg cgcaacata tggagcaca tgttcggcg taaaattat ggtttgtga tgttggagc agtttttaa tgaaatatg tgtggtctg gcacgggga tgattgtgg tttcgttgc cgattttat tatctagtg ggcctggga aagccccta ctgagggcg aaatttccg ataatgtgg ccgaaagga cgcattact tcaccacgg caaaaaatc tactgatta cgggttcaa atgcagttg agagattaa tgtcaagtt ataaagata tcgtcgagt tggcgggac atacggcca gggtggcca acatgttca cggttttgg aggaggcga gtcaaggga tttatcaca agaccttgg ttgagaaga aggagaagt gtggcgact ttgaaattg aatttaaag gcgaccaac cactcgcta aaggcaaat taatcgtct cgaccacca atgaaatca tcctccgta acgtcccca tagtcaccc ccaattgtg acgtggtgt gcccctcct taagcctcg aattaaagc cgggccaac acttgctca ttacgggcc ctaatggct gcggaaaat ccagccttt tccgcattc taagtgggc tgtggccca tctatggag gcgaactcc acaccccca agaactcaa tgttctaca tcccccagc ggccataca tggtcatag gcaacttga gagaccaga tcatttatc cggacacag ccaccgaca tgatcaata aacaagtga gcgaggatg agctccgga aaatcatgc gattggtgc acttggagc acatcgtcg agagggaca ccttccacc aggtcaagg attggacgg atattttgt caggcggcg aaaagcagc gaatggcaa tcgcgcgat tgttttacc acaagccga agtacgcgc tgttggacg agtgcacgt cggcggtgt ccatagatg ttgaaagct tcatttatc aaacggcca ttgacatgg gaatcacac ttttgacaa ttacgcatc ggcccactc tttggaaat ttcacactc atattttgc aatttgacg gcacaggtg cgtggaagt ttgaagaat tgaatgcca ataataggc tcagtttga agaaggaga aagaggaat taatgaaga agaaaagta ctgaagaaa ccattcaaa gactcaatg aactgaaca aacttttag gcgaagatg actagatcg tgtagttca atagctcta gctggtgaa ttgacagag atatcaatt aaagttgat ttatttat
