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Gene information
General information
TC015333 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 332575..337542
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC015333
    iBeetle screen iB_02462

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015333 RA
      MPTVVSKFL SKGEAALNV NRRALSGAV VAAALFAYT 
YKIAYPLLT RKTDVNINN NLVRRNIQN GLKSNKLPK 
RHRLRNTIP ALNLQFILQ FIKLARIMI PSVFCAEIG 
LLGGHTTFL FLRTFLSIY VANLEGAIV KYIVRKEPK 
NFIKQLVKW FAVAIPATF INSMIKYLE SKIALSFRT 
RLVEHSYQL YFRNQSYYR VTVLDGRLD NCAQRLTDD 
IETVASTVS HLYGQITKP MFDILLMAI ALANLVKSR 
NANLITGPA IISGVVIFS ALILRLVSP RFGQLVAQE 
AEKKGYLRH VHSRIVSNA EEIAFYGGH KVEQSQLRQ 
AFRILAQHM EHMFGVKLW FVMLEQFLM KYVWSGTGM 
IVVSLPILL SSGPGKAPT EGEISDNVA ERTHYFTTA 
KNLLITGSN AVERLMSSY KDIVELAGH TARVANMFT 
VLEEASQGI YHKTLVEKK EKCGDFEIE FKGDQPLAK 
GKLIVSTTN EIILRNVPI VTPNCDVVC PSLSLELKP 
GQHLLITGP NGCGKSSLF RILSGLWPI YGGELHTPK 
NSMFYIPQR PYMVIGNLR DQIIYPDTA TDMINKQVS 
EDELRKIMR LVHLEHIVE RDTFHQVKD WTDILSGGE 
KQRMAIARL FYHKPKYAL LDECTSAVS IDVESFIYQ 
TAIDMGITL LTITHRPTL WKFHTHILQ FDGTGAWKF 
EELNANNRL SLKKEKEEL MKKKSTEET IQRLNELNK 
LLGEDD
    CDS sequence
    • >TC015333 RA
      atgccaacg gtcgtgtca aaattcttg tccaagggc 
gaggcagcc ctgaatgtg aaccgaagg gcgctttcg 
ggggccgtg gtggccgcg gcccttttt gcctacacg 
tacaaaatc gcctatcct cttctaaca cgtaaaacg 
gacgtaaac atcaacaat aatctagtc agaagaaat 
atccaaaat gggctcaaa agcaacaaa ttgcccaaa 
agacaccgc ttacgtaat accatcccg gccctgaat 
ctacaattt attttgcaa tttatcaag ttggcgagg 
attatgatt ccgagtgtt ttttgtgcc gagattggc 
cttctggga gggcacacg acgtttttg tttttgcgg 
acgtttttg agcatttat gtggcgaac ttggagggg 
gccattgtc aaatatatc gtaagaaaa gagccgaaa 
aattttatc aaacagttg gttaagtgg tttgctgtt 
gcaattccg gccactttt atcaacagc atgatcaaa 
tatttggag agcaagatt gcgctgagc ttcaggacg 
cgattagtg gaacattcg taccaacta tacttcagg 
aaccaaagt tattacagg gtcaccgtc ctggacgga 
cgcctggat aattgcgcc cagcgtctc accgacgac 
attgagacc gtggccagc accgtttcg cacctttac 
ggccagatc accaagccc atgttcgac atcctcctc 
atggccatc gcgttggcc aacttggtc aaaagtcgc 
aacgccaac ttgatcaca ggccccgcc atcatctca 
ggtgttgtg attttctca gcgttgatt ttacggttg 
gtttcgcca cgttttggc caacttgtg gcccaggag 
gctgagaaa aagggttat ttacgtcat gtgcactct 
cgaattgtt tcaaacgcc gaagaaatc gccttttac 
ggcggtcat aaagtcgaa caatcgcaa ctgcgccaa 
gccttccgc attttggcg caacatatg gagcacatg 
ttcggcgta aaattatgg tttgtgatg ttggagcag 
tttttaatg aaatatgtg tggtctggc acggggatg 
attgtggtt tcgttgccg attttatta tctagtggg 
cctgggaaa gcccctact gagggcgaa atttccgat 
aatgtggcc gaaaggacg cattacttc accacggca 
aaaaatcta ctgattacg ggttcaaat gcagttgag 
agattaatg tcaagttat aaagatatc gtcgagttg 
gcgggacat acggccagg gtggccaac atgttcacg 
gttttggag gaggcgagt caagggatt tatcacaag 
accttggtt gagaagaag gagaagtgt ggcgacttt 
gaaattgaa tttaaaggc gaccaacca ctcgctaaa 
ggcaaatta atcgtctcg accaccaat gaaatcatc 
ctccgtaac gtccccata gtcaccccc aattgtgac 
gtggtgtgc ccctcctta agcctcgaa ttaaagccg 
ggccaacac ttgctcatt acgggccct aatggctgc 
ggaaaatcc agccttttc cgcattcta agtgggctg 
tggcccatc tatggaggc gaactccac acccccaag 
aactcaatg ttctacatc ccccagcgg ccatacatg 
gtcataggc aacttgaga gaccagatc atttatccg 
gacacagcc accgacatg atcaataaa caagtgagc 
gaggatgag ctccggaaa atcatgcga ttggtgcac 
ttggagcac atcgtcgag agggacacc ttccaccag 
gtcaaggat tggacggat attttgtca ggcggcgaa 
aagcagcga atggcaatc gcgcgattg ttttaccac 
aagccgaag tacgcgctg ttggacgag tgcacgtcg 
gcggtgtcc atagatgtt gaaagcttc atttatcaa 
acggccatt gacatggga atcacactt ttgacaatt 
acgcatcgg cccactctt tggaaattt cacactcat 
attttgcaa tttgacggc acaggtgcg tggaagttt 
gaagaattg aatgccaat aataggctc agtttgaag 
aaggagaaa gaggaatta atgaagaag aaaagtact 
gaagaaacc attcaaaga ctcaatgaa ctgaacaaa 
cttttaggc gaagatgac tag
    mRNA sequence
    • >TC015333 RA
      atctgtcac tttcgctct ttagtcaag cggcgatcg 
tcatttgtt gttgtaacc attggaaat atgtaacaa 
gtttgtaag caaaaaact agtgtgctg tggctcttt 
gtgtaacat caagatagc cgtgacaac gcttccggt 
tttttatca caggaaaat gccaacttg attggtgaa 
aaatgccaa cggtcgtgt caaaattct tgtccaagg 
gcgaggcag ccctgaatg tgaaccgaa gggcgcttt 
cgggggccg tggtggccg cggcccttt ttgcctaca 
cgtacaaaa tcgcctatc ctcttctaa cacgtaaaa 
cggacgtaa acatcaaca ataatctag tcagaagaa 
atatccaaa atgggctca aaagcaaca aattgccca 
aaagacacc gcttacgta ataccatcc cggccctga 
atctacaat ttattttgc aatttatca agttggcga 
ggattatga ttccgagtg ttttttgtg ccgagattg 
gccttctgg gagggcaca cgacgtttt tgtttttgc 
ggacgtttt tgagcattt atgtggcga acttggagg 
gggccattg tcaaatata tcgtaagaa aagagccga 
aaaatttta tcaaacagt tggttaagt ggtttgctg 
ttgcaattc cggccactt ttatcaaca gcatgatca 
aatatttgg agagcaaga ttgcgctga gcttcagga 
cgcgattag tggaacatt cgtaccaac tatacttca 
ggaaccaaa gttattaca gggtcaccg tcctggacg 
gacgcctgg ataattgcg cccagcgtc tcaccgacg 
acattgaga ccgtggcca gcaccgttt cgcaccttt 
acggccaga tcaccaagc ccatgttcg acatcctcc 
tcatggcca tcgcgttgg ccaacttgg tcaaaagtc 
gcaacgcca acttgatca caggccccg ccatcatct 
caggtgttg tgattttct cagcgttga ttttacggt 
tggtttcgc cacgttttg gccaacttg tggcccagg 
aggctgaga aaaagggtt atttacgtc atgtgcact 
ctcgaattg tttcaaacg ccgaagaaa tcgcctttt 
acggcggtc ataaagtcg aacaatcgc aactgcgcc 
aagccttcc gcattttgg cgcaacata tggagcaca 
tgttcggcg taaaattat ggtttgtga tgttggagc 
agtttttaa tgaaatatg tgtggtctg gcacgggga 
tgattgtgg tttcgttgc cgattttat tatctagtg 
ggcctggga aagccccta ctgagggcg aaatttccg 
ataatgtgg ccgaaagga cgcattact tcaccacgg 
caaaaaatc tactgatta cgggttcaa atgcagttg 
agagattaa tgtcaagtt ataaagata tcgtcgagt 
tggcgggac atacggcca gggtggcca acatgttca 
cggttttgg aggaggcga gtcaaggga tttatcaca 
agaccttgg ttgagaaga aggagaagt gtggcgact 
ttgaaattg aatttaaag gcgaccaac cactcgcta 
aaggcaaat taatcgtct cgaccacca atgaaatca 
tcctccgta acgtcccca tagtcaccc ccaattgtg 
acgtggtgt gcccctcct taagcctcg aattaaagc 
cgggccaac acttgctca ttacgggcc ctaatggct 
gcggaaaat ccagccttt tccgcattc taagtgggc 
tgtggccca tctatggag gcgaactcc acaccccca 
agaactcaa tgttctaca tcccccagc ggccataca 
tggtcatag gcaacttga gagaccaga tcatttatc 
cggacacag ccaccgaca tgatcaata aacaagtga 
gcgaggatg agctccgga aaatcatgc gattggtgc 
acttggagc acatcgtcg agagggaca ccttccacc 
aggtcaagg attggacgg atattttgt caggcggcg 
aaaagcagc gaatggcaa tcgcgcgat tgttttacc 
acaagccga agtacgcgc tgttggacg agtgcacgt 
cggcggtgt ccatagatg ttgaaagct tcatttatc 
aaacggcca ttgacatgg gaatcacac ttttgacaa 
ttacgcatc ggcccactc tttggaaat ttcacactc 
atattttgc aatttgacg gcacaggtg cgtggaagt 
ttgaagaat tgaatgcca ataataggc tcagtttga 
agaaggaga aagaggaat taatgaaga agaaaagta 
ctgaagaaa ccattcaaa gactcaatg aactgaaca 
aacttttag gcgaagatg actagatcg tgtagttca 
atagctcta gctggtgaa ttgacagag atatcaatt 
aaagttgat ttatttat
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