Status
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Gene information
General information
TC015325 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 431599..442866
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015325
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02460

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015325 RA
      MATYSRLLF RAVRIYWRA IFVVVYPLV LLPVFLHNN 
      VAAFRCLYV VLLMAGYWV FEALPLPVT SLIPMVLFP 
      LMGILDSDK TSLCYLKET NMMFVGGLV IAIAVEYCN 
      LHTRVALYV IQLVGCSPR KLNFGLVTV TMFVSMWIS 
      NTAATAMMI PIIEATLKE LETQGIGEM YESNSLDDN 
      DKIESVDSD PKKPTRTTM CYFISTAYA ASIGGMGCI 
      VGSGTNLTF KGIYETSFP ESPGIEFAK WMLLNVPMM 
      ILMMYLSLI WLQTMFMGL FRPNSQDAK KIRVGSQGA 
      AVARKLIRT KINEMGPMS FHEGAVAAL FCLSVLLWF 
      FRKPQFIVG WAELITQHK VKDATAALI VVLLLFIIP 
      ARPDFIYIL SKDESKRPK TPSPPLITW KVIQHKLPW 
      GLIFLLGGG FALAEASKE SQMSKLIAD HLHGMAELP 
      KFAVMVISC VFATVLTQF SSNVAVANV LLPVLAEMA 
      KVAKVHPLY LMLPTSLCC SFAYCLPVS TPPNAIAAA 
      PCNMSSTDM VKAGLGVAV ISLLVLFAV FPFLGAAIW 
      DLDTYPEWV E
    • >TC015325 RB
      MKINELLIG KQSQGFSTV RRNRTKITL RSPPNQSFK 
      VELDNTTLI KPCVRQLRP SLSRPTCEQ TSAKMKTFD 
      MATYSRLLF RAVRIYWRA IFVVVYPLV LLPVFLHNN 
      VAAFRCLYV VLLMAGYWV FEALPLPVT SLIPMVLFP 
      LMGILDSDK TSLCYLKET NMMFVGGLV IAIAVEYCN 
      LHTRVALYV IQLVGCSPR KLNFGLVTV TMFVSMWIS 
      NTAATAMMI PIIEATLKE LETQGIGEM YESNSLDDN 
      DKIESVDSD PKKPTRTTM CYFISTAYA ASIGGMGCI 
      VGSGTNLTF KGIYETSFP ESPGIEFAK WMLLNVPMM 
      ILMMYLSLI WLQTMFMGL FRPNSQDAK KIRVGSQGA 
      AVARKLIRT KINEMGPMS FHEGAVAAL FCLSVLLWF 
      FRKPQFIVG WAELITQHK VKDATAALI VVLLLFIIP 
      ARPDFIYIL SKDESKRPK TPSPPLITW KVIQHKLPW 
      GLIFLLGGG FALAEASKE SQMSKLIAD HLHGMAELP 
      KFAVMVISC VFATVLTQF SSNVAVANV LLPVLAEMA 
      KVAKVHPLY LMLPTSLCC SFAYCLPVS TPPNAIAAA 
      PCNMSSTDM VKAGLGVAV ISLLVLFAV FPFLGAAIW 
      DLDTYPEWV E
    CDS sequence
    • >TC015325 RA
      atggccacc tacagccgg ctgctgttc cgcgctgtg 
      cgcatctac tggagggcc atcttcgtg gtggtgtat 
      cctctggtg ctcctgccg gtgttcctg cacaacaat 
      gtggccgcc ttcaggtgc ctgtacgtg gtgctgctg 
      atggccggc tactgggtg tttgaggcg ctgccgctt 
      ccggtcact tcgctcatt cccatggtg ctgtttccg 
      ctcatgggc atcttggat tcggacaag acgtcgctg 
      tgttatctc aaggagacg aacatgatg tttgtgggc 
      gggctggtg attgccatc gctgtggag tattgcaat 
      ctgcacacg agggtggcc ttgtatgtg atacagttg 
      gtgggctgt agccccagg aagctaaac tttggcctg 
      gtcacggtc acaatgttc gtctcaatg tggatctca 
      aacactgcc gccacagca atgatgatt ccaatcatc 
      gaagccaca ctcaaagaa ctcgaaacg caaggaatc 
      ggcgaaatg tacgaatca aactcattg gacgacaat 
      gacaaaatc gaatcggtt gattccgac cctaaaaag 
      cccactcga acaacaatg tgctacttt atcagcact 
      gcctacgct gccagcatt gggggaatg ggctgtatc 
      gtaggaagt ggcaccaac ttaactttc aaggggatt 
      tacgagacc agtttcccg gaaagcccc ggaattgag 
      ttcgccaaa tggatgctg ctcaacgtg cccatgatg 
      attttaatg atgtacttg tccctcatc tggctccag 
      acgatgttc atgggcttg ttccgtccc aactcccaa 
      gacgccaaa aaaatccgc gttggcagt caaggcgcg 
      gccgtggcg cgtaagcta atccgaacc aaaataaac 
      gaaatgggc cccatgtcc ttccacgag ggggctgtt 
      gcagcctta ttctgcctc tcagtgttg ctttggttc 
      ttccgaaag ccgcagttt atagtaggc tgggccgaa 
      cttataacc cagcacaag gtcaaggac gccacagcc 
      gccttgatc gtcgttctt ttgctgttt ataatccca 
      gcacgtcca gatttcatc tacattttg agcaaggac 
      gagtccaaa cggccgaaa acgccctca cccccgtta 
      ataacctgg aaggtcatc cagcataag ctcccctgg 
      gggctgatt ttcctgctc gggggcggt ttcgcgctg 
      gccgaagcc tcgaaagag agccagatg agtaaacta 
      atagctgac catttgcac ggaatggcg gaattgccg 
      aaattcgct gtgatggtc atttcgtgc gtgtttgcc 
      acggttctg acgcagttt tccagcaac gttgccgtg 
      gcaaatgtg ctgttgccg gttttggcc gaaatggcc 
      aaagtggca aaagtgcac cccctgtat ctaatgctg 
      ccaacgtcg ctgtgctgc tcgttcgcc tattgcctg 
      ccggtgagc acgcccccg aacgccatc gccgccgcc 
      ccctgcaac atgtcctcc accgatatg gtgaaggcg 
      ggcctgggg gtggcggtc atttcgctg ctggtgctg 
      ttcgccgtg ttccccttc ctgggggcg gcgatttgg 
      gatttggac acgtatccg gagtgggtg gagtga
    • >TC015325 RB
      atgaaaatc aatgaatta ttaatcggg aaacaaagc 
      caaggcttt tctactgtc agacggaac cgaacaaag 
      attactctg aggtcacca ccaaaccaa tcattcaaa 
      gtcgagctt gataacacc acacttata aagccatgc 
      gtccgccaa cttcgaccc agtctctct cgcccaaca 
      tgcgaacag acctccgcc aaaatgaaa acgttcgac 
      atggccacc tacagccgg ctgctgttc cgcgctgtg 
      cgcatctac tggagggcc atcttcgtg gtggtgtat 
      cctctggtg ctcctgccg gtgttcctg cacaacaat 
      gtggccgcc ttcaggtgc ctgtacgtg gtgctgctg 
      atggccggc tactgggtg tttgaggcg ctgccgctt 
      ccggtcact tcgctcatt cccatggtg ctgtttccg 
      ctcatgggc atcttggat tcggacaag acgtcgctg 
      tgttatctc aaggagacg aacatgatg tttgtgggc 
      gggctggtg attgccatc gctgtggag tattgcaat 
      ctgcacacg agggtggcc ttgtatgtg atacagttg 
      gtgggctgt agccccagg aagctaaac tttggcctg 
      gtcacggtc acaatgttc gtctcaatg tggatctca 
      aacactgcc gccacagca atgatgatt ccaatcatc 
      gaagccaca ctcaaagaa ctcgaaacg caaggaatc 
      ggcgaaatg tacgaatca aactcattg gacgacaat 
      gacaaaatc gaatcggtt gattccgac cctaaaaag 
      cccactcga acaacaatg tgctacttt atcagcact 
      gcctacgct gccagcatt gggggaatg ggctgtatc 
      gtaggaagt ggcaccaac ttaactttc aaggggatt 
      tacgagacc agtttcccg gaaagcccc ggaattgag 
      ttcgccaaa tggatgctg ctcaacgtg cccatgatg 
      attttaatg atgtacttg tccctcatc tggctccag 
      acgatgttc atgggcttg ttccgtccc aactcccaa 
      gacgccaaa aaaatccgc gttggcagt caaggcgcg 
      gccgtggcg cgtaagcta atccgaacc aaaataaac 
      gaaatgggc cccatgtcc ttccacgag ggggctgtt 
      gcagcctta ttctgcctc tcagtgttg ctttggttc 
      ttccgaaag ccgcagttt atagtaggc tgggccgaa 
      cttataacc cagcacaag gtcaaggac gccacagcc 
      gccttgatc gtcgttctt ttgctgttt ataatccca 
      gcacgtcca gatttcatc tacattttg agcaaggac 
      gagtccaaa cggccgaaa acgccctca cccccgtta 
      ataacctgg aaggtcatc cagcataag ctcccctgg 
      gggctgatt ttcctgctc gggggcggt ttcgcgctg 
      gccgaagcc tcgaaagag agccagatg agtaaacta 
      atagctgac catttgcac ggaatggcg gaattgccg 
      aaattcgct gtgatggtc atttcgtgc gtgtttgcc 
      acggttctg acgcagttt tccagcaac gttgccgtg 
      gcaaatgtg ctgttgccg gttttggcc gaaatggcc 
      aaagtggca aaagtgcac cccctgtat ctaatgctg 
      ccaacgtcg ctgtgctgc tcgttcgcc tattgcctg 
      ccggtgagc acgcccccg aacgccatc gccgccgcc 
      ccctgcaac atgtcctcc accgatatg gtgaaggcg 
      ggcctgggg gtggcggtc atttcgctg ctggtgctg 
      ttcgccgtg ttccccttc ctgggggcg gcgatttgg 
      gatttggac acgtatccg gagtgggtg gagtga
    mRNA sequence
    • >TC015325 RA
      atcagttgg tggcaaagg tgcgctcag tgcagttac 
      cgcaaaaat gctatgaaa atcaatgaa ttattaatc 
      gggaaacaa agccaaggc ttttctact gtcagtgaa 
      aacgttcga catggccac ctacagccg gctgctgtt 
      ccgcgctgt gcgcatcta ctggagggc catcttcgt 
      ggtggtgta tcctctggt gctcctgcc ggtgttcct 
      gcacaacaa tgtggccgc cttcaggtg cctgtacgt 
      ggtgctgct gatggccgg ctactgggt gtttgaggc 
      gctgccgct tccggtcac ttcgctcat tcccatggt 
      gctgtttcc gctcatggg catcttgga ttcggacaa 
      gacgtcgct gtgttatct caaggagac gaacatgat 
      gtttgtggg cgggctggt gattgccat cgctgtgga 
      gtattgcaa tctgcacac gagggtggc cttgtatgt 
      gatacagtt ggtgggctg tagccccag gaagctaaa 
      ctttggcct ggtcacggt cacaatgtt cgtctcaat 
      gtggatctc aaacactgc cgccacagc aatgatgat 
      tccaatcat cgaagccac actcaaaga actcgaaac 
      gcaaggaat cggcgaaat gtacgaatc aaactcatt 
      ggacgacaa tgacaaaat cgaatcggt tgattccga 
      ccctaaaaa gcccactcg aacaacaat gtgctactt 
      tatcagcac tgcctacgc tgccagcat tgggggaat 
      gggctgtat cgtaggaag tggcaccaa cttaacttt 
      caaggggat ttacgagac cagtttccc ggaaagccc 
      cggaattga gttcgccaa atggatgct gctcaacgt 
      gcccatgat gattttaat gatgtactt gtccctcat 
      ctggctcca gacgatgtt catgggctt gttccgtcc 
      caactccca agacgccaa aaaaatccg cgttggcag 
      tcaaggcgc ggccgtggc gcgtaagct aatccgaac 
      caaaataaa cgaaatggg ccccatgtc cttccacga 
      gggggctgt tgcagcctt attctgcct ctcagtgtt 
      gctttggtt cttccgaaa gccgcagtt tatagtagg 
      ctgggccga acttataac ccagcacaa ggtcaagga 
      cgccacagc cgccttgat cgtcgttct tttgctgtt 
      tataatccc agcacgtcc agatttcat ctacatttt 
      gagcaagga cgagtccaa acggccgaa aacgccctc 
      acccccgtt aataacctg gaaggtcat ccagcataa 
      gctcccctg ggggctgat tttcctgct cgggggcgg 
      tttcgcgct ggccgaagc ctcgaaaga gagccagat 
      gagtaaact aatagctga ccatttgca cggaatggc 
      ggaattgcc gaaattcgc tgtgatggt catttcgtg 
      cgtgtttgc cacggttct gacgcagtt ttccagcaa 
      cgttgccgt ggcaaatgt gctgttgcc ggttttggc 
      cgaaatggc caaagtggc aaaagtgca ccccctgta 
      tctaatgct gccaacgtc gctgtgctg ctcgttcgc 
      ctattgcct gccggtgag cacgccccc gaacgccat 
      cgccgccgc cccctgcaa catgtcctc caccgatat 
      ggtgaaggc gggcctggg ggtggcggt catttcgct 
      gctggtgct gttcgccgt gttcccctt cctgggggc 
      ggcgatttg ggatttgga cacgtatcc ggagtgggt 
      ggagtgaaa agactgacg tcgtgatga tgatttttt 
      ttggttttt tatttgacg atgtattat atttatgga 
      ctatttaat taagtgtac agatttttg attccattt 
      cgaataaaa ttgataaga attt
    • >TC015325 RB
      atcagttgg tggcaaagg tgcgctcag tgcagttac 
      cgcaaaaat gctatgaaa atcaatgaa ttattaatc 
      gggaaacaa agccaaggc ttttctact gtcagacgg 
      aaccgaaca aagattact ctgaggtca ccaccaaac 
      caatcattc aaagtcgag cttgataac accacactt 
      ataaagcca tgcgtccgc caacttcga cccagtctc 
      tctcgccca acatgcgaa cagacctcc gccaaaatg 
      aaaacgttc gacatggcc acctacagc cggctgctg 
      ttccgcgct gtgcgcatc tactggagg gccatcttc 
      gtggtggtg tatcctctg gtgctcctg ccggtgttc 
      ctgcacaac aatgtggcc gccttcagg tgcctgtac 
      gtggtgctg ctgatggcc ggctactgg gtgtttgag 
      gcgctgccg cttccggtc acttcgctc attcccatg 
      gtgctgttt ccgctcatg ggcatcttg gattcggac 
      aagacgtcg ctgtgttat ctcaaggag acgaacatg 
      atgtttgtg ggcgggctg gtgattgcc atcgctgtg 
      gagtattgc aatctgcac acgagggtg gccttgtat 
      gtgatacag ttggtgggc tgtagcccc aggaagcta 
      aactttggc ctggtcacg gtcacaatg ttcgtctca 
      atgtggatc tcaaacact gccgccaca gcaatgatg 
      attccaatc atcgaagcc acactcaaa gaactcgaa 
      acgcaagga atcggcgaa atgtacgaa tcaaactca 
      ttggacgac aatgacaaa atcgaatcg gttgattcc 
      gaccctaaa aagcccact cgaacaaca atgtgctac 
      tttatcagc actgcctac gctgccagc attggggga 
      atgggctgt atcgtagga agtggcacc aacttaact 
      ttcaagggg atttacgag accagtttc ccggaaagc 
      cccggaatt gagttcgcc aaatggatg ctgctcaac 
      gtgcccatg atgatttta atgatgtac ttgtccctc 
      atctggctc cagacgatg ttcatgggc ttgttccgt 
      cccaactcc caagacgcc aaaaaaatc cgcgttggc 
      agtcaaggc gcggccgtg gcgcgtaag ctaatccga 
      accaaaata aacgaaatg ggccccatg tccttccac 
      gagggggct gttgcagcc ttattctgc ctctcagtg 
      ttgctttgg ttcttccga aagccgcag tttatagta 
      ggctgggcc gaacttata acccagcac aaggtcaag 
      gacgccaca gccgccttg atcgtcgtt cttttgctg 
      tttataatc ccagcacgt ccagatttc atctacatt 
      ttgagcaag gacgagtcc aaacggccg aaaacgccc 
      tcacccccg ttaataacc tggaaggtc atccagcat 
      aagctcccc tgggggctg attttcctg ctcgggggc 
      ggtttcgcg ctggccgaa gcctcgaaa gagagccag 
      atgagtaaa ctaatagct gaccatttg cacggaatg 
      gcggaattg ccgaaattc gctgtgatg gtcatttcg 
      tgcgtgttt gccacggtt ctgacgcag ttttccagc 
      aacgttgcc gtggcaaat gtgctgttg ccggttttg 
      gccgaaatg gccaaagtg gcaaaagtg caccccctg 
      tatctaatg ctgccaacg tcgctgtgc tgctcgttc 
      gcctattgc ctgccggtg agcacgccc ccgaacgcc 
      atcgccgcc gccccctgc aacatgtcc tccaccgat 
      atggtgaag gcgggcctg ggggtggcg gtcatttcg 
      ctgctggtg ctgttcgcc gtgttcccc ttcctgggg 
      gcggcgatt tgggatttg gacacgtat ccggagtgg 
      gtggagtga aaagactga cgtcgtgat gatgatttt 
      ttttggttt tttatttga cgatgtatt atatttatg 
      gactattta attaagtgt acagatttt tgattccat 
      ttcgaataa aattgataa gaattt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY