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Gene information
General information
TC015324 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 464310..477596
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015324
    iBeetle screen iB_02459

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 11.11% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    Pupal morphology
    head slightly orientation irregular - Number of animals showing the phenotype: 7

    antenna slightlyorientation irregular - Number of animals showing the phenotype: 7
    Comment: probably secondarily
    wings shortened - Number of animals showing the phenotype: 7

    Adult morphology
    elytra shortened - Number of animals showing the phenotype: 6


    elytra distal not closed - Number of animals showing the phenotype: 6


    mesothoracic leg femur slightly shortened - Number of animals showing the phenotype: 6


    abdomen slightly shortened - Number of animals showing the phenotype: 5


    abdominal odoriferous glands size decreased - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection


    Comment: darker color in one animals stink glands is a result of denaturation due to dissection buffer

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015324 RA
      MALKLRKDI KKSSYYVWF LGAKESKGL RGSEYVLPV 
      IKHLEAREK DIEPFKVTL QVSHKGLKI VQNVVSQKG 
      TKPKNETIK HFIPHNTIT CVYQHEDVI ACILLLFNP 
      VTKCPLHVH AYRCDSLET ATLLKQQLQ TLIERPENQ 
      KKFAEIEGR LKGAKEGRK GESSVGSDT GTSTRESES 
      SEERYTVET TRVTTLYDS VAAELREKL AKNNVPILL 
      PPRDYDTVH RQKGNLTGI ELRRCLNAN IVGQNAKGR 
      RLESSGGSS GIGSDHPPS PDSPDAPDT RFNTLDNQS 
      TSDEDWNGD PADSTLYLM HAPPQVTLP RPNREKSPD 
      SPRYYNQSK PTPRDIAKY LEKPISKYV ERHEKPMKY 
      FDDDGFDEN LRYRNRPRY DFPPVPKTR QRIDPHYYP 
      QRSPSPVET SPRDRFKDA KEKFLSMEK ERLTREPPI 
      SPIKRHESM MYAPKPAPR HASERDAPI GRYRNDKYD 
      PKRRSMFSL IEEEHRKNS NEIAREIKR RSYMDDDVR 
      DTNYNLSKS TVELNQKGG YRHSYAEPK LRVEKGGGK 
      KHFEMLHRT NSVTNNGRV GIASVHPY
    CDS sequence
    • >TC015324 RA
      atggcccta aaactgcgc aaagacatc aaaaaatcc 
      tcctactac gtttggttt ctcggtgcc aaagagtcc 
      aaaggcctc cgaggctcc gaatacgtc cttcccgtc 
      atcaagcac ctggaggcg cgcgaaaag gacatcgag 
      cccttcaag gtcaccctc caagtctcg cacaaaggc 
      ctcaaaatc gtccaaaat gtcgtctcc caaaagggc 
      accaaaccc aagaacgaa accatcaaa catttcatc 
      ccccacaac actataacg tgtgtctac cagcacgag 
      gacgtgatt gcgtgtatt ttgctgctt ttcaatcca 
      gtgaccaag tgtcccttg cacgtgcac gcctacaga 
      tgtgacagt ttggagacg gcaactttg ttaaaacag 
      caacttcag actttgatc gaaaggccg gaaaatcag 
      aagaagttt gcggagatt gaggggcgg ctgaagggg 
      gcgaaggag gggcggaag ggggagtcc tcggtgggg 
      agcgacacc ggcaccagc acaagggag agtgagtcg 
      agtgaagaa agatatact gttgaaaca acccgagtt 
      acaaccctt tacgactca gtggcggcg gaactgcgc 
      gaaaaactg gccaaaaac aacgtcccg atccttctg 
      cccccgcgc gactacgac actgtgcac cgccaaaag 
      ggcaatttg acgggaatt gagttgagg cggtgtctc 
      aacgcaaat atcgtcggt caaaatgcc aaaggacga 
      cggctggaa agcagtggc ggcagctca gggattggg 
      tcagatcac cccccaagt cctgattct cccgacgcc 
      cctgacacc agattcaat actttggat aatcaaagc 
      acgagcgat gaagattgg aacggggac ccggctgac 
      tccaccctc tacctaatg cacgccccg ccccaagtg 
      accttgccc cgccccaat cgcgagaaa tcccccgac 
      tcgcctcgc tactacaac caatcaaaa cccacccca 
      cgtgacatc gccaaatac ctcgaaaaa ccaatctca 
      aaatacgtg gaacgtcac gaaaaacca atgaaatac 
      ttcgacgac gacggcttt gacgaaaac ttacgttac 
      aggaaccgc ccccgttac gacttcccc ccagtaccg 
      aaaacacgc caacgaatc gatccccac tactacccc 
      cagcgtagc ccctcccct gtggaaacc tccccacgt 
      gaccgcttc aaggacgct aaagaaaaa ttcctgtca 
      atggaaaaa gagcgtttg acacgcgaa ccgcccatt 
      tcgccaatc aaacgccac gaaagcatg atgtacgca 
      cctaagccc gcccctcgc cacgcctcc gaacgcgac 
      gcgccaatc ggccgctac cgaaacgac aaatacgac 
      ccgaaaagg cggtccatg tttagcttg atcgaggag 
      gagcatcgg aaaaactcc aatgagatt gcgcgcgag 
      atcaagagg cggagctac atggacgat gacgtcaga 
      gacacgaat tataatttg tcgaaaagc acagtcgag 
      ttgaatcaa aagggcggg tataggcat agttacgct 
      gaaccgaaa ttaagggtg gagaagggt gggggtaag 
      aaacacttc gagatgttg cacagaacc aatagcgtc 
      acgaataat gggagggtg gggattgca agtgtgcat 
      ccctattaa
    mRNA sequence
    • >TC015324 RA
      ccacagcca actcgacat acctgttga attcagaag 
      gtgatggtg attggaaga acgtaacac agttctcca 
      tctgtgtga aaaaaagca atttttggt gaaattctg 
      gcttaagtg gcggttttt tccgggaaa ttgtgataa 
      tcgtgtgat gtttgatgt gtctttagt gtgattttc 
      acggatttt tgggcttgt gaccctagt gccataaaa 
      aaatgctcg agccctttt aaaaaaaat ggccctaaa 
      actgcgcaa agacatcaa aaaatcctc ctactacgt 
      ttggtttct cggtgccaa agagtccaa aggcctccg 
      aggctccga atacgtcct tcccgtcat caagcacct 
      ggaggcgcg cgaaaagga catcgagcc cttcaaggt 
      caccctcca agtctcgca caaaggcct caaaatcgt 
      ccaaaatgt cgtctccca aaagggcac caaacccaa 
      gaacgaaac catcaaaca tttcatccc ccacaacac 
      tataacgtg tgtctacca gcacgagga cgtgattgc 
      gtgtatttt gctgctttt caatccagt gaccaagtg 
      tcccttgca cgtgcacgc ctacagatg tgacagttt 
      ggagacggc aactttgtt aaaacagca acttcagac 
      tttgatcga aaggccgga aaatcagaa gaagtttgc 
      ggagattga ggggcggct gaagggggc gaaggaggg 
      gcggaaggg ggagtcctc ggtggggag cgacaccgg 
      caccagcac aagggagag tgagtcgag tgaagaaag 
      atatactgt tgaaacaac ccgagttac aacccttta 
      cgactcagt ggcggcgga actgcgcga aaaactggc 
      caaaaacaa cgtcccgat ccttctgcc cccgcgcga 
      ctacgacac tgtgcaccg ccaaaaggg caatttgac 
      gggaattga gttgaggcg gtgtctcaa cgcaaatat 
      cgtcggtca aaatgccaa aggacgacg gctggaaag 
      cagtggcgg cagctcagg gattgggtc agatcaccc 
      cccaagtcc tgattctcc cgacgcccc tgacaccag 
      attcaatac tttggataa tcaaagcac gagcgatga 
      agattggaa cggggaccc ggctgactc caccctcta 
      cctaatgca cgccccgcc ccaagtgac cttgccccg 
      ccccaatcg cgagaaatc ccccgactc gcctcgcta 
      ctacaacca atcaaaacc caccccacg tgacatcgc 
      caaatacct cgaaaaacc aatctcaaa atacgtgga 
      acgtcacga aaaaccaat gaaatactt cgacgacga 
      cggctttga cgaaaactt acgttacag gaaccgccc 
      ccgttacga cttcccccc agtaccgaa aacacgcca 
      acgaatcga tccccacta ctaccccca gcgtagccc 
      ctcccctgt ggaaacctc cccacgtga ccgcttcaa 
      ggacgctaa agaaaaatt cctgtcaat ggaaaaaga 
      gcgtttgac acgcgaacc gcccatttc gccaatcaa 
      acgccacga aagcatgat gtacgcacc taagcccgc 
      ccctcgcca cgcctccga acgcgacgc gccaatcgg 
      ccgctaccg aaacgacaa atacgaccc gaaaaggcg 
      gtccatgtt tagcttgat cgaggagga gcatcggaa 
      aaactccaa tgagattgc gcgcgagat caagaggcg 
      gagctacat ggacgatga cgtcagaga cacgaatta 
      taatttgtc gaaaagcac agtcgagtt gaatcaaaa 
      gggcgggta taggcatag ttacgctga accgaaatt 
      aagggtgga gaagggtgg gggtaagaa acacttcga 
      gatgttgca cagaaccaa tagcgtcac gaataatgg 
      gagggtggg gattgcaag tgtgcatcc ctattaatt 
      gtacatagg ggagaccat gacatgtta gttcatagt 
      gtaatatat ttatttgtc ttgatgtgc cttttttaa 
      acaagggga gcgcgattt agggaagtt tggttttct 
      gtgattggc tcctcggta acaaagcaa ttaatgttt 
      tttttaatt atttatcgt tttatataa tttatgtat 
      ttctggttt tgtatatta ttgtatcag atgtctatt 
      taaacgcaa attttattt taataaata atgattttt 
      aca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY