Status
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Gene information
General information
TC015323 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 502335..507729
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015323
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02458

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015323 RA
      MQKENTNPS PEIHQYVGP YRLEKTLGK GQTGLVKLG 
      VNCVTGKKV AIKIINREK LSESVLMKV EREIAIMKL 
      IDHPHVLGL TDVYENKKY LYLVLEHVS GGELFDYLV 
      KKGRLTPKE ARRFFRQII SALDFCHSH SICHRDLKP 
      ENLLLDEKN NIKIADFGM ASLQPMGSM LETSCGSPH 
      YACPEVIRG EKYDGRKAD VWSCGVILY ALLVGALPF 
      DDDNLRQLL EKVKRGVFH IPHFVPPDC QSLLRGMIE 
      VNPEKRLTL SEINKHPWV TAGGKGELE LELPMMEVV 
      QTHVLPSIE AVDPDVLQA ICSLGCFKE KEKLIQHLL 
      SPNHNTEKV IYFLLLERK RRRPAYEDD TESILRMRV 
      SETSDPPKK RIDTCRVNG QSGLQFGQI SEGSPLTPR 
      RSHYKRHHA RRSPSTGST HSSLGIHSP TRSSGSSSP 
      VMFNSTTTP INTHSGMSS PVQRAHRSS NTHVTVTPP 
      PATPTHHRA NSSGGTATS SVLTSPESD SLGVGPPSI 
      LTAMTPPGS PHSTATSHH WRSRLTTIK NSFLGSPRF 
      HRRKLQTSS EEVHLTPES SPELTKKSW FGSLMTTEK 
      DETFTILVK GKPLATVKA DLIHAFLSI AELSHSVSS 
      PMSFRVEYK RGTTGPAMF QRHVRFQVD ISTITKQVG 
      DNAKEHLYA ITFTLLSGN IRRFRRVCE HIQAQVCTR 
      RPPPSPRAQ RKFTTELSE SSSCGSDSS ELYLNARQL 
      NVLQLEGSD LESECSVFD VRTAAREVS RRASTNNNT 
      EACEQNTPG TPKAVRSNS ENTDSCEKT CITTMSGSS 
      IA
    CDS sequence
    • >TC015323 RA
      atgcagaag gaaaacacg aacccttcg ccggagata 
      caccagtac gtggggccc taccggctg gagaagacg 
      ctcggaaag ggacaaacg ggtcttgtc aaacttgga 
      gtaaactgc gtgactgga aaaaaggtc gcgattaaa 
      ataatcaat cgcgagaaa ctcagtgag agcgtctta 
      atgaaagta gagcgtgaa atagcgata atgaagctt 
      atcgaccac ccccacgtc ttggggttg actgacgtt 
      tacgaaaat aagaaatat ttgtaccta gttttagag 
      cacgtctca ggcggcgaa cttttcgat tatctagtc 
      aaaaaaggt agactcaca cctaaggag gctcgcaga 
      tttttccgc cagatcata tccgccctt gatttctgc 
      cacagccac tcgatttgc catcgggac ctcaagccg 
      gaaaatctc ctcctggac gaaaaaaac aacataaaa 
      atcgccgat tttggcatg gcgtcgtta caaccaatg 
      gggtccatg ttggagaca agttgtggg tccccccat 
      tatgcatgt cccgaagtt atacggggg gagaagtac 
      gatgggagg aaagcagac gtgtggtca tgtggggtg 
      attttgtac gctctttta gtaggggct ttgcccttc 
      gatgatgat aatttgaga cagttgttg gagaaagtg 
      aaaaggggg gtgtttcat ataccgcat tttgtacca 
      cccgactgt caaagtttg ttaaggggg atgattgaa 
      gtcaatccg gaaaaaaga cttacgctt tctgagatc 
      aataaacac ccatgggtg acggccggt ggtaaaggc 
      gagcttgaa ctcgaatta cccatgatg gaagtggtc 
      caaacccat gtcttacca tcgattgag gccgtcgat 
      cctgacgta ttgcaagcc atctgtagt ctgggctgt 
      ttcaaagag aaagagaag ttgattcaa cacctacta 
      agtccaaac cacaacacc gaaaaagtc atctatttc 
      ctcctcctc gagcgcaaa cgccgtcgt cccgcctac 
      gaagacgac accgaatca atcctgagg atgcgcgtg 
      tccgaaacg tcggaccct ccgaaaaag cgcatcgac 
      acgtgtcgt gtcaacggc caaagcggc ctccaattc 
      ggacaaata agcgaaggt tcgccttta acgccccgc 
      cgctcgcac tacaagcgc caccacgcg aggcggagc 
      ccctccaca ggctccacc cacagcagt ctgggcatc 
      cactcgccg acgagatcg tccggctcg tccagccct 
      gttatgttc aatagtacg accacgccc atcaatacg 
      cactccgga atgtcgtcg ccggtgcaa cgcgcgcac 
      cgctcgtcc aacacccac gtgactgtg accccgcct 
      ccggccacg cctacgcac catcgggcc aatagcagt 
      ggcggcacg gcgacgtcg tccgtactg acgtcaccc 
      gaaagtgat agtttaggg gtggggcca ccgtcgatt 
      ttgacagcg atgactccg cccggttcg ccacattcg 
      acagcgacg agccaccac tggcggtcg aggcttacc 
      acgatcaag aacagtttt ttgggctcg ccaaggttt 
      caccgccgg aaattgcaa acgtcgtcg gaggaagtg 
      catttaacg ccggaatcg tcgcccgaa ttgacgaaa 
      aaatcctgg tttgggagc ctgatgacg accgaaaag 
      gacgaaacg tttacgatt ttggtcaaa gggaagcct 
      ctggcgacg gtcaaggcc gacctgatt cacgcattt 
      ttgtcgata gccgagctg agccactcc gtgtcgagt 
      ccgatgtcg ttccgcgtg gaatacaag cggggaacc 
      accggtccg gccatgttc cagcgccat gtccgtttc 
      caagtcgat atttcaaca ataacaaag caagtcggt 
      gacaatgca aaagaacac ctttacgct atcactttc 
      actctctta tcaggaaac atccggcgt ttccggcgc 
      gtttgcgaa cacatccag gcgcaagtg tgcacccgc 
      cggccccca ccgagcccg agggcgcag cgcaagttc 
      accaccgaa ctctccgaa agttcgagc tgtggctcc 
      gattccagc gaactgtac ctcaacgcc cggcagttg 
      aacgttttg caactggaa ggcagcgat ctagagagc 
      gaatgtagc gtgtttgac gtgcgcacc gctgcgcgg 
      gaggtgtca cgacgggcc tccaccaat aacaacaca 
      gaagcgtgc gaacagaac acgccgggg actcctaag 
      gccgttcgg tccaattcg gaaaatacg gattcgtgc 
      gaaaagacg tgcattacg acgatgtcg gggagttcc 
      atagcgtaa
    mRNA sequence
    • >TC015323 RA
      tgacgacat gcaaaaagg gctgtgagt gacgacgac 
      agtcacccg acaaaatgc agaaggaaa acacgaacc 
      cttcgccgg agatacacc agtacgtgg ggccctacc 
      ggctggaga agacgctcg gaaagggac aaacgggtc 
      ttgtcaaac ttggagtaa actgcgtga ctggaaaaa 
      aggtcgcga ttaaaataa tcaatcgcg agaaactca 
      gtgagagcg tcttaatga aagtagagc gtgaaatag 
      cgataatga agcttatcg accaccccc acgtcttgg 
      ggttgactg acgtttacg aaaataaga aatatttgt 
      acctagttt tagagcacg tctcaggcg gcgaacttt 
      tcgattatc tagtcaaaa aaggtagac tcacaccta 
      aggaggctc gcagatttt tccgccaga tcatatccg 
      cccttgatt tctgccaca gccactcga tttgccatc 
      gggacctca agccggaaa atctcctcc tggacgaaa 
      aaaacaaca taaaaatcg ccgattttg gcatggcgt 
      cgttacaac caatggggt ccatgttgg agacaagtt 
      gtgggtccc cccattatg catgtcccg aagttatac 
      ggggggaga agtacgatg ggaggaaag cagacgtgt 
      ggtcatgtg gggtgattt tgtacgctc ttttagtag 
      gggctttgc ccttcgatg atgataatt tgagacagt 
      tgttggaga aagtgaaaa ggggggtgt ttcatatac 
      cgcattttg taccacccg actgtcaaa gtttgttaa 
      gggggatga ttgaagtca atccggaaa aaagactta 
      cgctttctg agatcaata aacacccat gggtgacgg 
      ccggtggta aaggcgagc ttgaactcg aattaccca 
      tgatggaag tggtccaaa cccatgtct taccatcga 
      ttgaggccg tcgatcctg acgtattgc aagccatct 
      gtagtctgg gctgtttca aagagaaag agaagttga 
      ttcaacacc tactaagtc caaaccaca acaccgaaa 
      aagtcatct atttcctcc tcctcgagc gcaaacgcc 
      gtcgtcccg cctacgaag acgacaccg aatcaatcc 
      tgaggatgc gcgtgtccg aaacgtcgg accctccga 
      aaaagcgca tcgacacgt gtcgtgtca acggccaaa 
      gcggcctcc aattcggac aaataagcg aaggttcgc 
      ctttaacgc cccgccgct cgcactaca agcgccacc 
      acgcgaggc ggagcccct ccacaggct ccacccaca 
      gcagtctgg gcatccact cgccgacga gatcgtccg 
      gctcgtcca gccctgtta tgttcaata gtacgacca 
      cgcccatca atacgcact ccggaatgt cgtcgccgg 
      tgcaacgcg cgcaccgct cgtccaaca cccacgtga 
      ctgtgaccc cgcctccgg ccacgccta cgcaccatc 
      gggccaata gcagtggcg gcacggcga cgtcgtccg 
      tactgacgt cacccgaaa gtgatagtt taggggtgg 
      ggccaccgt cgattttga cagcgatga ctccgcccg 
      gttcgccac attcgacag cgacgagcc accactggc 
      ggtcgaggc ttaccacga tcaagaaca gttttttgg 
      gctcgccaa ggtttcacc gccggaaat tgcaaacgt 
      cgtcggagg aagtgcatt taacgccgg aatcgtcgc 
      ccgaattga cgaaaaaat cctggtttg ggagcctga 
      tgacgaccg aaaaggacg aaacgttta cgattttgg 
      tcaaaggga agcctctgg cgacggtca aggccgacc 
      tgattcacg catttttgt cgatagccg agctgagcc 
      actccgtgt cgagtccga tgtcgttcc gcgtggaat 
      acaagcggg gaaccaccg gtccggcca tgttccagc 
      gccatgtcc gtttccaag tcgatattt caacaataa 
      caaagcaag tcggtgaca atgcaaaag aacaccttt 
      acgctatca ctttcactc tcttatcag gaaacatcc 
      ggcgtttcc ggcgcgttt gcgaacaca tccaggcgc 
      aagtgtgca cccgccggc ccccaccga gcccgaggg 
      cgcagcgca agttcacca ccgaactct ccgaaagtt 
      cgagctgtg gctccgatt ccagcgaac tgtacctca 
      acgcccggc agttgaacg ttttgcaac tggaaggca 
      gcgatctag agagcgaat gtagcgtgt ttgacgtgc 
      gcaccgctg cgcgggagg tgtcacgac gggcctcca 
      ccaataaca acacagaag cgtgcgaac agaacacgc 
      cggggactc ctaaggccg ttcggtcca attcggaaa 
      atacggatt cgtgcgaaa agacgtgca ttacgacga 
      tgtcgggga gttccatag cgtaatttg gggggtaag 
      gggtggcca aagcgactg ctttattat tattattat 
      cactgttat tcggccact tttcccgta ttattatta 
      ttgttatta ttattacta aaggcataa cctcattat 
      tagcggatt gcaatataa tatattatc aaaaatttg 
      acatatgtc actattgtc atccgtgtg tgacagttt 
      tttgggcag cgtcgccta ctagacact tttgttcct 
      gcgcaccgg cggccattt tggatgatt agaagtgga 
      gaattgtta tgacgttgc ttttgtaaa tatgctgaa 
      agcttatgt gattttgga tatcgttca gtgtagttg 
      tataaattg ttaaaagaa agaaataaa tgtcaaaca 
      aaaactaac cccaggtag gtaggtgaa caaaaaaat 
      gacattctc tgatgtaca tataactac actatactc 
      agctgagtc gtttcatat ttccccgtt tcagaatca 
      aacaatacg acttaaaaa aactatata aaattgtat 
      ttagagaaa ttgccggcc atattgtca actaatatt 
      tagggtata ggtgataat aaataaaac atttcgagt 
      cgcatcata atttcgatt ttgtgtgtc cttgacata 
      tcaatgcat ttttcagct caatatgtt tacgttgtg 
      gcagtttga caacgggga cactttgta cctttgtaa 
      atatcaatt caattctcg tgtaaattc atttcaaac 
      aactcattt ttctattga ctagtttta gttgaaaca 
      cactgtttt tgttttgtg ttttgcaca catacgtta 
      gtgttatag tgaattatt ttatataac aaaggaaac 
      taaagtgaa tatagttta tgtgtaagt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY