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Gene information
General information
TC015322 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 516898..523584
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015322
    iBeetle screen iB_02457

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 9 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015322 RA
      MLGAVARAG SSLLAGKTI EKLGSESLK VVSVYNGSQ 
      RGLAAKAAA SGKPNGKVV AVIGAVVDV QFDDNLPPI 
      LNALEVQNR SPRLVLEVA QHLGENTVR TIAMDGTEG 
      LVRGQAVSD TGYPIRIPV GAETLGRII NVIGEPIDE 
      RGPVPTDKL APIHAEAPE FVEMSVEQE ILVTGIKVV 
      DLLAPYAKG GKIGLFGGA GVGKTVLIM ELINNVAKA 
      HGGYSVFAG VGERTREGN DLYHEMIES GVISLKDKT 
      SKVALVYGQ MNEPPGARA RVALTGLTV AEYFRDQEG 
      QDVLLFIDN IFRFTQAGS EVSALLGRI PSAVGYQPT 
      LATDMGSMQ ERITTTKKG SITSVQAIY VPADDLTDP 
      APATTFAHL DATTVLSRA IAELGIYPA VDPLDSTSR 
      IMDPNIIGQ EHYNVARGV QKILQDYKS LQDIIAILG 
      MDELSEDDK LTVARARKI QRFLSQPFQ VAEVFTGHA 
      GKLVPLQET IKGFQKILG GEYDHLPEV AFYMVGPIE 
      EVVQKAEKL AESTA
    CDS sequence
    • >TC015322 RA
      atgttgggt gctgtggca agagctgga tccagcctt 
      ttggctgga aaaaccatc gaaaaactt ggaagtgag 
      tcgctgaaa gtcgtctcc gtgtacaat ggatcccaa 
      cgtggtttg gctgccaag gccgccgcc tccggcaaa 
      cccaacggt aaagtggtt gccgtaatc ggtgccgtc 
      gtcgacgtc caattcgac gacaacctt cccccaatc 
      cttaacgcc cttgaagtc caaaaccgt tccccacgt 
      cttgtcctg gaagtcgcc caacatttg ggtgaaaac 
      accgtccgt accatcgcc atggacggt accgaaggt 
      ctcgtacgt ggacaagcc gtcagcgat accggctat 
      cccatccgt atcccagtc ggcgctgaa acccttggc 
      cgcattata aacgtcatt ggtgaaccc attgacgaa 
      agaggtcca gtaccaacc gataaactt gcacccatt 
      catgctgaa gctcccgaa ttcgttgaa atgagtgtc 
      gagcaagag attctcgtc actgggatt aaagttgtt 
      gatttgttg gcaccgtat gccaagggc gggaaaatt 
      gggcttttt ggaggcgct ggagtcggg aagactgtg 
      ctgattatg gagttgatc aataatgtg gctaaggca 
      catggtggg tattccgtg tttgccgga gttggggag 
      aggaccaga gaagggaat gatttgtac catgaaatg 
      atcgagtct ggtgttatt tccttgaag gataagaca 
      tcgaaggta gcgttggtg tatggacaa atgaacgaa 
      ccaccaggc gccagagct agggtggct ttgaccgga 
      ttgacagtc gctgaatat ttcagggat caagaagga 
      caagatgtg ctgttgttt attgataat attttcagg 
      ttcacccaa gctggttct gaagtatca gctctgttg 
      ggtcgtatc ccctctgct gtgggctac cagccgact 
      ttagccact gacatgggt agcatgcag gaacgaatt 
      accaccacg aaaaagggc tcaatcacg tcagtccag 
      gccatttac gtgcccgct gacgattta actgacccc 
      gcccctgcg acaacattc gcccacttg gacgccaca 
      actgtcctg tcccgtgct attgccgaa ttgggcatt 
      tacccggcc gtggatccc ctggattcc acctcgcgt 
      attatggac cccaacatc atcgggcag gagcactac 
      aatgtggcc cgtggcgtc cagaaaatc ctccaggat 
      tataaatcg ctccaggat attattgcc atcttgggc 
      atggacgag ctgtctgaa gacgacaag ttgacggtg 
      gcacgtgcc aggaagatc cagcgtttc ctgtcgcag 
      ccgttccaa gtcgctgaa gttttcacc ggacatgct 
      ggcaagttg gtgccgttg caggagacc atcaaagga 
      ttccagaag attctagga ggggagtac gaccatttg 
      cccgaagtt gcgttctat atggtcggg cccattgaa 
      gaggtcgta cagaaagca gagaagtta gccgagtct 
      actgcttaa
    mRNA sequence
    • >TC015322 RA
      acctagcgg cgactttgc gaatcagag gtccattcg 
      agccagacg atgttgggt gctgtggca agagctgga 
      tccagcctt ttggctgga aaaaccatc gaaaaactt 
      ggaagtgag tcgctgaaa gtcgtctcc gtgtacaat 
      ggatcccaa cgtggtttg gctgccaag gccgccgcc 
      tccggcaaa cccaacggt aaagtggtt gccgtaatc 
      ggtgccgtc gtcgacgtc caattcgac gacaacctt 
      cccccaatc cttaacgcc cttgaagtc caaaaccgt 
      tccccacgt cttgtcctg gaagtcgcc caacatttg 
      ggtgaaaac accgtccgt accatcgcc atggacggt 
      accgaaggt ctcgtacgt ggacaagcc gtcagcgat 
      accggctat cccatccgt atcccagtc ggcgctgaa 
      acccttggc cgcattata aacgtcatt ggtgaaccc 
      attgacgaa agaggtcca gtaccaacc gataaactt 
      gcacccatt catgctgaa gctcccgaa ttcgttgaa 
      atgagtgtc gagcaagag attctcgtc actgggatt 
      aaagttgtt gatttgttg gcaccgtat gccaagggc 
      gggaaaatt gggcttttt ggaggcgct ggagtcggg 
      aagactgtg ctgattatg gagttgatc aataatgtg 
      gctaaggca catggtggg tattccgtg tttgccgga 
      gttggggag aggaccaga gaagggaat gatttgtac 
      catgaaatg atcgagtct ggtgttatt tccttgaag 
      gataagaca tcgaaggta gcgttggtg tatggacaa 
      atgaacgaa ccaccaggc gccagagct agggtggct 
      ttgaccgga ttgacagtc gctgaatat ttcagggat 
      caagaagga caagatgtg ctgttgttt attgataat 
      attttcagg ttcacccaa gctggttct gaagtatca 
      gctctgttg ggtcgtatc ccctctgct gtgggctac 
      cagccgact ttagccact gacatgggt agcatgcag 
      gaacgaatt accaccacg aaaaagggc tcaatcacg 
      tcagtccag gccatttac gtgcccgct gacgattta 
      actgacccc gcccctgcg acaacattc gcccacttg 
      gacgccaca actgtcctg tcccgtgct attgccgaa 
      ttgggcatt tacccggcc gtggatccc ctggattcc 
      acctcgcgt attatggac cccaacatc atcgggcag 
      gagcactac aatgtggcc cgtggcgtc cagaaaatc 
      ctccaggat tataaatcg ctccaggat attattgcc 
      atcttgggc atggacgag ctgtctgaa gacgacaag 
      ttgacggtg gcacgtgcc aggaagatc cagcgtttc 
      ctgtcgcag ccgttccaa gtcgctgaa gttttcacc 
      ggacatgct ggcaagttg gtgccgttg caggagacc 
      atcaaagga ttccagaag attctagga ggggagtac 
      gaccatttg cccgaagtt gcgttctat atggtcggg 
      cccattgaa gaggtcgta cagaaagca gagaagtta 
      gccgagtct actgcttaa tttcattat ttccatcta 
      ggtttcaaa gtcattttt tttatttgt aacgttgtc 
      aactgtaca tcatcgcat tataaggcg tatttgttg 
      taattacta ttgattcta agaaaagat gtaggacgt 
      tacatggtt attgtgttt tatttattt cctgcaata 
      aatttttga aggaata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY