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Gene information
General information
TC015321 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 523702..526754
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015321
    iBeetle screen iB_02456

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015321 RA
      MAEADKLNI DSIIARLLE VRGARPGKN VQLTESEIR 
      GLCLKSREI FLSQPILLE LEAPLKICG DIHGQYYDL 
      LRLFEYGGF PPESNYLFL GDYVDRGKQ SLETICLLL 
      AYKIKYPEN FFLLRGNHE CASINRIYG FYDECKRRY 
      NIKLWKTFT DCFNCLPVA AIVDEKIFC CHGGLSPDL 
      QSMEQIRRI MRPTDVPDQ GLLCDLLWS DPDKDQMGW 
      GENDRGVSF TFGAEVVGK FLHKHDFDL ICRAHQVVE 
      DGYEFFAKR QLVTLFSAP NYCGEFDNA GAMMSVDET 
      LMCSFQILK PADKRKFQY NMNAGRPVT PPRGGTNKN 
      KKK
    CDS sequence
    • >TC015321 RA
      atggctgaa gccgacaaa ttaaatatc gatagtatc 
      atagctcgg ctattggaa gtacgcgga gcaagacct 
      ggcaaaaat gtacaatta accgaaagc gaaatccgg 
      gggctgtgc ctcaagtcc cgggagatt ttcctctcg 
      cagccgatt ttactggaa ctggaggcg ccacttaag 
      atttgcggt gatatccac ggtcaatat tatgatctc 
      cttcgtctg ttcgaatac ggggggttt cccccagag 
      tcgaattac ctattcctt ggcgattac gtcgatcgt 
      gggaaacaa tcattggaa accatctgt cttttgctc 
      gcctacaaa ataaaatat cccgagaat ttcttcctt 
      cttagaggt aaccacgag tgcgcctca atcaaccgc 
      atatacgga ttctacgac gaatgtaaa aggaggtac 
      aatataaag ttatggaaa accttcacc gactgcttc 
      aactgcttg ccggtggcg gcgatcgtc gacgagaag 
      attttctgt tgtcatggt ggcctcagt cccgacctc 
      cagtcgatg gaacaaatc cgaaggata atgcgacca 
      acagacgtg cccgaccaa ggccttctc tgtgacctg 
      ttgtggtca gatcctgac aaagatcaa atgggctgg 
      ggtgaaaat gatcgtggt gttagtttt actttcggc 
      gccgaagtt gtgggaaaa ttcctccat aaacacgac 
      tttgatctg atttgtcga gcccatcaa gtcgttgag 
      gacgggtac gaatttttc gccaaacgg caacttgtc 
      acgcttttt agcgcccca aattattgc ggcgagttt 
      gataacgcg ggagcgatg atgtcagtg gacgaaacg 
      ttgatgtgc agttttcaa atcctgaag ccggccgac 
      aagcggaag tttcagtat aacatgaac gccggaagg 
      ccggtgacg ccacctaga ggaggcact aataaaaac 
      aagaagaag tga
    mRNA sequence
    • >TC015321 RA
      gcagcgctg cgcgcgcct aacttcagc ctccgcgcg 
      agtcgaggc gaaaaagta gtgtttatt tcactgagg 
      ctctccgaa gatcaaaaa aaatccgag ccatccggt 
      agttttaag tggtagaat tcgctctag ttaagcaca 
      aaaggtcgc aacaaatgg ctgaagccg acaaattaa 
      atatcgata gtatcatag ctcggctat tggaagtac 
      gcggagcaa gacctggca aaaatgtac aattaaccg 
      aaagcgaaa tccgggggc tgtgcctca agtcccggg 
      agattttcc tctcgcagc cgattttac tggaactgg 
      aggcgccac ttaagattt gcggtgata tccacggtc 
      aatattatg atctccttc gtctgttcg aatacgggg 
      ggtttcccc cagagtcga attacctat tccttggcg 
      attacgtcg atcgtggga aacaatcat tggaaacca 
      tctgtcttt tgctcgcct acaaaataa aatatcccg 
      agaatttct tccttctta gaggtaacc acgagtgcg 
      cctcaatca accgcatat acggattct acgacgaat 
      gtaaaagga ggtacaata taaagttat ggaaaacct 
      tcaccgact gcttcaact gcttgccgg tggcggcga 
      tcgtcgacg agaagattt tctgttgtc atggtggcc 
      tcagtcccg acctccagt cgatggaac aaatccgaa 
      ggataatgc gaccaacag acgtgcccg accaaggcc 
      ttctctgtg acctgttgt ggtcagatc ctgacaaag 
      atcaaatgg gctggggtg aaaatgatc gtggtgtta 
      gttttactt tcggcgccg aagttgtgg gaaaattcc 
      tccataaac acgactttg atctgattt gtcgagccc 
      atcaagtcg ttgaggacg ggtacgaat ttttcgcca 
      aacggcaac ttgtcacgc tttttagcg ccccaaatt 
      attgcggcg agtttgata acgcgggag cgatgatgt 
      cagtggacg aaacgttga tgtgcagtt ttcaaatcc 
      tgaagccgg ccgacaagc ggaagtttc agtataaca 
      tgaacgccg gaaggccgg tgacgccac ctagaggag 
      gcactaata aaaacaaga agaagtgaa tataattta 
      tttttaaga ttaggatag ctcttcgtc tataatttt 
      aatcatttt aaatactaa tactgtact gtttcaagt 
      aatgtatga aataaggtc taaaatttg ttttcttgt 
      cacacttat ttaagtatt aaatcacgt gatgttaga 
      ataacgcct actatgtga tcgtttcat ccaagtact 
      gttagtatt taaaatgga gctgaggcc atttttggg 
      ttattttta gttgatata aatcgtgta atttattta 
      aaccaggat gtgaacgag gtgatgatc atatcaaat 
      acaaataat ggaaaatgg ctctaatca gattcaaac 
      atttacaat aggctgtat ttgttttta ttttggttt 
      ttatccatc ttgtttgtt tctttgacg tttaaaagg 
      cgattctat ttttctata tgtaagggt taagtttca 
      cgtttggcc aaaatgaaa atgaatggt ggcaactta 
      ttatttttt atccaaccg ttcgttccg ttgacattt 
      gtacataat tgcgttcca aaaccacct gtttctttg 
      atctgcgta acttttgtc tttaataaa gattttaac 
      aattt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY