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Gene information
General information
TC015321 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 523702..526754
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC015321
    iBeetle screen iB_02456

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015321 RA
      MAEADKLNI DSIIARLLE VRGARPGKN VQLTESEIR 
GLCLKSREI FLSQPILLE LEAPLKICG DIHGQYYDL 
LRLFEYGGF PPESNYLFL GDYVDRGKQ SLETICLLL 
AYKIKYPEN FFLLRGNHE CASINRIYG FYDECKRRY 
NIKLWKTFT DCFNCLPVA AIVDEKIFC CHGGLSPDL 
QSMEQIRRI MRPTDVPDQ GLLCDLLWS DPDKDQMGW 
GENDRGVSF TFGAEVVGK FLHKHDFDL ICRAHQVVE 
DGYEFFAKR QLVTLFSAP NYCGEFDNA GAMMSVDET 
LMCSFQILK PADKRKFQY NMNAGRPVT PPRGGTNKN 
KKK
    CDS sequence
    • >TC015321 RA
      atggctgaa gccgacaaa ttaaatatc gatagtatc 
atagctcgg ctattggaa gtacgcgga gcaagacct 
ggcaaaaat gtacaatta accgaaagc gaaatccgg 
gggctgtgc ctcaagtcc cgggagatt ttcctctcg 
cagccgatt ttactggaa ctggaggcg ccacttaag 
atttgcggt gatatccac ggtcaatat tatgatctc 
cttcgtctg ttcgaatac ggggggttt cccccagag 
tcgaattac ctattcctt ggcgattac gtcgatcgt 
gggaaacaa tcattggaa accatctgt cttttgctc 
gcctacaaa ataaaatat cccgagaat ttcttcctt 
cttagaggt aaccacgag tgcgcctca atcaaccgc 
atatacgga ttctacgac gaatgtaaa aggaggtac 
aatataaag ttatggaaa accttcacc gactgcttc 
aactgcttg ccggtggcg gcgatcgtc gacgagaag 
attttctgt tgtcatggt ggcctcagt cccgacctc 
cagtcgatg gaacaaatc cgaaggata atgcgacca 
acagacgtg cccgaccaa ggccttctc tgtgacctg 
ttgtggtca gatcctgac aaagatcaa atgggctgg 
ggtgaaaat gatcgtggt gttagtttt actttcggc 
gccgaagtt gtgggaaaa ttcctccat aaacacgac 
tttgatctg atttgtcga gcccatcaa gtcgttgag 
gacgggtac gaatttttc gccaaacgg caacttgtc 
acgcttttt agcgcccca aattattgc ggcgagttt 
gataacgcg ggagcgatg atgtcagtg gacgaaacg 
ttgatgtgc agttttcaa atcctgaag ccggccgac 
aagcggaag tttcagtat aacatgaac gccggaagg 
ccggtgacg ccacctaga ggaggcact aataaaaac 
aagaagaag tga
    mRNA sequence
    • >TC015321 RA
      gcagcgctg cgcgcgcct aacttcagc ctccgcgcg 
agtcgaggc gaaaaagta gtgtttatt tcactgagg 
ctctccgaa gatcaaaaa aaatccgag ccatccggt 
agttttaag tggtagaat tcgctctag ttaagcaca 
aaaggtcgc aacaaatgg ctgaagccg acaaattaa 
atatcgata gtatcatag ctcggctat tggaagtac 
gcggagcaa gacctggca aaaatgtac aattaaccg 
aaagcgaaa tccgggggc tgtgcctca agtcccggg 
agattttcc tctcgcagc cgattttac tggaactgg 
aggcgccac ttaagattt gcggtgata tccacggtc 
aatattatg atctccttc gtctgttcg aatacgggg 
ggtttcccc cagagtcga attacctat tccttggcg 
attacgtcg atcgtggga aacaatcat tggaaacca 
tctgtcttt tgctcgcct acaaaataa aatatcccg 
agaatttct tccttctta gaggtaacc acgagtgcg 
cctcaatca accgcatat acggattct acgacgaat 
gtaaaagga ggtacaata taaagttat ggaaaacct 
tcaccgact gcttcaact gcttgccgg tggcggcga 
tcgtcgacg agaagattt tctgttgtc atggtggcc 
tcagtcccg acctccagt cgatggaac aaatccgaa 
ggataatgc gaccaacag acgtgcccg accaaggcc 
ttctctgtg acctgttgt ggtcagatc ctgacaaag 
atcaaatgg gctggggtg aaaatgatc gtggtgtta 
gttttactt tcggcgccg aagttgtgg gaaaattcc 
tccataaac acgactttg atctgattt gtcgagccc 
atcaagtcg ttgaggacg ggtacgaat ttttcgcca 
aacggcaac ttgtcacgc tttttagcg ccccaaatt 
attgcggcg agtttgata acgcgggag cgatgatgt 
cagtggacg aaacgttga tgtgcagtt ttcaaatcc 
tgaagccgg ccgacaagc ggaagtttc agtataaca 
tgaacgccg gaaggccgg tgacgccac ctagaggag 
gcactaata aaaacaaga agaagtgaa tataattta 
tttttaaga ttaggatag ctcttcgtc tataatttt 
aatcatttt aaatactaa tactgtact gtttcaagt 
aatgtatga aataaggtc taaaatttg ttttcttgt 
cacacttat ttaagtatt aaatcacgt gatgttaga 
ataacgcct actatgtga tcgtttcat ccaagtact 
gttagtatt taaaatgga gctgaggcc atttttggg 
ttattttta gttgatata aatcgtgta atttattta 
aaccaggat gtgaacgag gtgatgatc atatcaaat 
acaaataat ggaaaatgg ctctaatca gattcaaac 
atttacaat aggctgtat ttgttttta ttttggttt 
ttatccatc ttgtttgtt tctttgacg tttaaaagg 
cgattctat ttttctata tgtaagggt taagtttca 
cgtttggcc aaaatgaaa atgaatggt ggcaactta 
ttatttttt atccaaccg ttcgttccg ttgacattt 
gtacataat tgcgttcca aaaccacct gtttctttg 
atctgcgta acttttgtc tttaataaa gattttaac 
aattt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY