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Gene information
General information
TC015315 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid39: 584442..600689
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC015315
      iBeetle screen iB_02451
      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

      larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      larva random constricted - Penetrance (percentage of animals/eggs on slide): less than 30%

      procephalic head irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
      Days post injection


      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC015315 RA
        MGKKGRKTR WRTLTITDE HSDSEESNT TTSGHRLPK 
        GYPHKSYYS RFTYSSQST PTRRYQCDG TKSTRSSST 
        ASENKITFN EDEYTRITT PRQDVLFKK GYLNKPKTY 
        QTQTSTGNS TTSTGNSTG NGTPDHQST DLEYESQFV 
        FPNGFVDQN GIYYINSFE PYPVMVYNP PTYYQEFTN 
        IKAKRCSTG SLTESTSPN NEETSQSET PEVNPVYNV 
        VYPNYYCNG GVYEEPQNG AISEPEARR VKKRRRRKP 
        SRSKEGTTT SESSDEGEE EEEEEEEKE VFSEKEQQK 
        TYDLKPDAE EFVPRAYRQ PPETIPMEA ANVQFIKIP 
        SNFVPVPIL SDFNAFIPP GIPINFIPQ NFINYVPLK 
        AAVQSDDGK AATDDTVKE LATVEVPKN QIDIAKIVS 
        KLEEAAKEQ DKDETTPNH KAKKRHRYQ KVELRKPPP 
        SPAEPFKPP LRNYSETLK KIPLKTNER SEEKKTLVK 
        EPVKDHRSE QTPPKVTNE WISVSSRKK RKNKNVEEI 
        EDFEEVEAA PPSEEKVEP VVIIQPPPP PPPPPPPPE 
        PEPVITVKK VKKKTKKVV APPPITKRV IIKDVDLSV 
        EEVKEEPKP VVEEAPPPK KEEKVEEIV EKKESPEKK 
        TKKKKKKVG KAATIVNSQ SSSNTTLNV TDDSYDFLM 
        DESMEKTNV EVSQELDKL IQRGMYNNL EEKIKSMNI 
        ESNDNFFKS IITNISSSR EPSVEKPYI KTPDLSKIL 
        PNQALLKPR KPGDFLGES PTPSPLGGG SPPPSDNLY 
        PITKAVKEW MSKTRESAP DIELLKSPS EIFREFTER 
        EDDDDVTSD EDCRTVQAE QTGKYPKHG NLPYRAICC 
        SIM
      CDS sequence
      • >TC015315 RA
        atgggaaag aagggtcgc aagaccagg tggcgcact 
        ttaactatc acagatgag cacagcgat agtgaagaa 
        tccaacacg acaacatcg ggacaccga ttacccaaa 
        ggctaccca cacaagtca tactacagc cgttttacg 
        tattctagt caatcaacg cccaccaga aggtaccag 
        tgcgatggt acgaagtcg acaaggtcc agcagcact 
        gccagtgaa aataaaatc accttcaat gaagacgaa 
        tatacgcgc attacgaca cctagacaa gatgtatta 
        tttaaaaag ggttacttg aataaaccc aaaacgtat 
        caaacgcag acctcgacc ggaaattcg acgacttcg 
        actggtaat tccaccgga aacggaaca ccagaccac 
        caatcaaca gatctagaa tacgaatca cagttcgtc 
        ttcccgaat ggtttcgtc gatcaaaac ggaatctac 
        tatatcaac agttttgaa ccgtatcca gtgatggtt 
        tacaatcct ccgacctat tatcaagaa tttacgaac 
        attaaagcg aaaagatgc agcacagga tcgttgacg 
        gaatcgacg agccccaac aacgaggaa actagtcaa 
        agcgagaca cccgaagtg aatccagtc tacaatgtt 
        gtttatccg aattattac tgtaacggc ggtgtttac 
        gaggagccc cagaatggg gctatcagt gagcctgaa 
        gcccgtcgt gtgaagaaa cgaagacgg cgaaagccg 
        tcacggtcg aaagagggc accaccacg tctgaatct 
        tccgatgaa ggagaagaa gaagaagag gaagaagaa 
        gaaaaagaa gttttcagc gagaaagaa caacaaaaa 
        acgtacgat ctcaaaccg gatgcggaa gagtttgtc 
        cctcgggcc taccgccag ccccctgag accattccg 
        atggaagca gccaacgtt caattcatc aaaattccg 
        tcgaatttc gttccggtg ccgatcttg agcgatttt 
        aacgcattt attccgcca gggattccg atcaacttt 
        atacctcag aactttatc aattatgta cctttgaaa 
        gcggccgtt caatcagac gatggtaaa gcagcgacg 
        gacgacact gttaaagaa ctagcaacg gttgaagtt 
        cctaaaaat caaatcgac attgcaaaa atcgtatcc 
        aagctcgaa gaagcagcc aaagaacaa gacaaagat 
        gaaacaaca ccgaaccat aaagcgaaa aagagacac 
        cgataccag aaagttgaa ctgagaaaa ccaccacca 
        agtcccgca gaaccgttc aaaccacca ctccgtaac 
        tattccgaa actttgaag aaaattccg cttaaaacg 
        aacgaacgt agcgaggaa aaaaaaaca ctggttaaa 
        gaaccagtc aaggatcat cgaagtgaa caaacgcca 
        ccgaaagtg acaaatgag tggatttcg gtgtcgagt 
        cgtaaaaaa cgaaagaac aaaaatgtg gaagaaatt 
        gaagatttt gaagaagtt gaagcagcg cccccttct 
        gaagaaaaa gttgaaccg gttgttatt attcaacca 
        ccacctcca ccaccacca ccaccacca ccaccggaa 
        ccagagcca gttattaca gtgaagaaa gttaagaag 
        aagacgaag aaagtggtg gctccgccc ccaattacc 
        aaacgagtg attatcaag gacgttgat ttgtcggtg 
        gaagaagtg aaggaggag cctaaaccg gttgtggag 
        gaagcaccg ccccccaaa aaagaagaa aaggttgaa 
        gaaattgtg gaaaagaag gaatctccc gaaaagaaa 
        acgaagaag aagaagaag aaagttggc aaggctgct 
        acgattgtg aatagccaa tccagctcg aatacgacg 
        cttaatgtg acagatgat tcctatgat tttctcatg 
        gatgagtcg atggagaag actaacgtt gaggtgtcg 
        caagagctg gacaaactc atccagcgc ggaatgtac 
        aacaatctc gaagagaaa atcaaatca atgaacatt 
        gaatcaaac gacaacttc ttcaaaagc ataatcacc 
        aacatatca agcagtcgg gagccaagt gtggaaaaa 
        ccgtacata aaaaccccc gacttgagc aaaatcctc 
        ccaaaccaa gcgctcctc aagccacga aaaccgggc 
        gatttttta ggtgaaagc cccaccccc agcccactc 
        gggggtggt tccccgccc cccagcgac aatctctac 
        cccatcacg aaagccgtg aaggagtgg atgagcaag 
        acgcgcgaa agcgccccc gacatcgag ttactcaaa 
        agtccgagc gagattttt cgggaattc accgaaagg 
        gaggacgac gatgacgta acaagcgac gaagattgc 
        cgaacggtg caagccgag caaacgggg aaatacccc 
        aaacacggc aacttgccc tacagggcg atttgctgc 
        agtattatg tga
      mRNA sequence
      • >TC015315 RA
        ccgaaggcg tccggaggc gtccgatcg attacaaaa 
        tagtgaaga agcgccggt ccggcgcag agtgtttta 
        gtggctatg cggagacaa tttagtcga taacgagcg 
        ctccgagtt acgtaatgt aatatcact ggagaattg 
        tcaaaaaaa tcaaacatg ctgtagacc gaatggggt 
        aacccccgg ttagtgact ggcgactgt ttcgggcac 
        cacgcggga ataattaat aagaaacag tgcgaaaaa 
        aataaattt gggtgagtg tctgatggt ggattaaat 
        ccgttcgaa accggtaga aaggaaaat gggaaagaa 
        gggtcgcaa gaccaggtg gcgcacttt aactatcac 
        agatgagca cagcgatag tgaagaatc caacacgac 
        aacatcggg acaccgatt acccaaagg ctacccaca 
        caagtcata ctacagccg ttttacgta ttctagtca 
        atcaacgcc caccagaag gtaccagtg cgatggtac 
        gaagtcgac aaggtccag cagcactgc cagtgaaaa 
        taaaatcac cttcaatga agacgaata tacgcgcat 
        tacgacacc tagacaaga tgtattatt taaaaaggg 
        ttacttgaa taaacccaa aacgtatca aacgcagac 
        ctcgaccgg aaattcgac gacttcgac tggtaattc 
        caccggaaa cggaacacc agaccacca atcaacaga 
        tctagaata cgaatcaca gttcgtctt cccgaatgg 
        tttcgtcga tcaaaacgg aatctacta tatcaacag 
        ttttgaacc gtatccagt gatggttta caatcctcc 
        gacctatta tcaagaatt tacgaacat taaagcgaa 
        aagatgcag cacaggatc gttgacgga atcgacgag 
        ccccaacaa cgaggaaac tagtcaaag cgagacacc 
        cgaagtgaa tccagtcta caatgttgt ttatccgaa 
        ttattactg taacggcgg tgtttacga ggagcccca 
        gaatggggc tatcagtga gcctgaagc ccgtcgtgt 
        gaagaaacg aagacggcg aaagccgtc acggtcgaa 
        agagggcac caccacgtc tgaatcttc cgatgaagg 
        agaagaaga agaagagga agaagaaga aaaagaagt 
        tttcagcga gaaagaaca acaaaaaac gtacgatct 
        caaaccgga tgcggaaga gtttgtccc tcgggccta 
        ccgccagcc ccctgagac cattccgat ggaagcagc 
        caacgttca attcatcaa aattccgtc gaatttcgt 
        tccggtgcc gatcttgag cgattttaa cgcatttat 
        tccgccagg gattccgat caactttat acctcagaa 
        ctttatcaa ttatgtacc tttgaaagc ggccgttca 
        atcagacga tggtaaagc agcgacgga cgacactgt 
        taaagaact agcaacggt tgaagttcc taaaaatca 
        aatcgacat tgcaaaaat cgtatccaa gctcgaaga 
        agcagccaa agaacaaga caaagatga aacaacacc 
        gaaccataa agcgaaaaa gagacaccg ataccagaa 
        agttgaact gagaaaacc accaccaag tcccgcaga 
        accgttcaa accaccact ccgtaacta ttccgaaac 
        tttgaagaa aattccgct taaaacgaa cgaacgtag 
        cgaggaaaa aaaaacact ggttaaaga accagtcaa 
        ggatcatcg aagtgaaca aacgccacc gaaagtgac 
        aaatgagtg gatttcggt gtcgagtcg taaaaaacg 
        aaagaacaa aaatgtgga agaaattga agattttga 
        agaagttga agcagcgcc cccttctga agaaaaagt 
        tgaaccggt tgttattat tcaaccacc acctccacc 
        accaccacc accaccacc accggaacc agagccagt 
        tattacagt gaagaaagt taagaagaa gacgaagaa 
        agtggtggc tccgccccc aattaccaa acgagtgat 
        tatcaagga cgttgattt gtcggtgga agaagtgaa 
        ggaggagcc taaaccggt tgtggagga agcaccgcc 
        ccccaaaaa agaagaaaa ggttgaaga aattgtgga 
        aaagaagga atctcccga aaagaaaac gaagaagaa 
        gaagaagaa agttggcaa ggctgctac gattgtgaa 
        tagccaatc cagctcgaa tacgacgct taatgtgac 
        agatgattc ctatgattt tctcatgga tgagtcgat 
        ggagaagac taacgttga ggtgtcgca agagctgga 
        caaactcat ccagcgcgg aatgtacaa caatctcga 
        agagaaaat caaatcaat gaacattga atcaaacga 
        caacttctt caaaagcat aatcaccaa catatcaag 
        cagtcggga gccaagtgt ggaaaaacc gtacataaa 
        aacccccga cttgagcaa aatcctccc aaaccaagc 
        gctcctcaa gccacgaaa accgggcga ttttttagg 
        tgaaagccc cacccccag cccactcgg gggtggttc 
        cccgccccc cagcgacaa tctctaccc catcacgaa 
        agccgtgaa ggagtggat gagcaagac gcgcgaaag 
        cgcccccga catcgagtt actcaaaag tccgagcga 
        gatttttcg ggaattcac cgaaaggga ggacgacga 
        tgacgtaac aagcgacga agattgccg aacggtgca 
        agccgagca aacggggaa ataccccaa acacggcaa 
        cttgcccta cagggcgat ttgctgcag tattatgtg 
        atggcggct gacgcgatt acttaattc tggacgtac 
        ttaagggct tgttctttt tatgtggcg cacacaacg 
        gttggtaac actgtttcc aaggcctgc tgcttcaca 
        cagagactg aatacgatg atgtttaag gcagggctt 
        ggtgtcata tgttcgtga aatgttgtt attgttaaa 
        tagttgtta ggggaagtc cggccgcgg tttaattat 
        gtagcggcg acgatttta gttggttca tcatcaaag 
        ccatttgct cattttatt ggttgttta ggatgtagt 
        gtgcaattt ttatttttt tttttcaag tgtgggtgg 
        gacacaccc ttaaagcgg cgacaatat tgttaaagg 
        ggtgtgaat ttttcaaca aaaaagaca ctatgtatg 
        ctaagtcta gtgttaagt ttgttttgc gcatgtatg 
        gagtttttt tcttaagtt tttctttcg tttggttgt 
        tctatatca gttatttta ttgttataa tgtttgtta 
        tttttgaga gttgtgcct ttgcaaaat ttttaaaat 
        aaagaaaat tataataa