Status
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Gene information
General information
TC015315 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 584442..600689
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      		TC015315
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes

      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
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      Publish name
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_02451

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection

      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

      larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
       larva random constricted - Penetrance (percentage of animals/eggs on slide): less than 30%

      procephalic head irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
      Days post injection

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
      Days post injection

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC015315 RA
        MGKKGRKTR WRTLTITDE HSDSEESNT TTSGHRLPK 
GYPHKSYYS RFTYSSQST PTRRYQCDG TKSTRSSST 
ASENKITFN EDEYTRITT PRQDVLFKK GYLNKPKTY 
QTQTSTGNS TTSTGNSTG NGTPDHQST DLEYESQFV 
FPNGFVDQN GIYYINSFE PYPVMVYNP PTYYQEFTN 
IKAKRCSTG SLTESTSPN NEETSQSET PEVNPVYNV 
VYPNYYCNG GVYEEPQNG AISEPEARR VKKRRRRKP 
SRSKEGTTT SESSDEGEE EEEEEEEKE VFSEKEQQK 
TYDLKPDAE EFVPRAYRQ PPETIPMEA ANVQFIKIP 
SNFVPVPIL SDFNAFIPP GIPINFIPQ NFINYVPLK 
AAVQSDDGK AATDDTVKE LATVEVPKN QIDIAKIVS 
KLEEAAKEQ DKDETTPNH KAKKRHRYQ KVELRKPPP 
SPAEPFKPP LRNYSETLK KIPLKTNER SEEKKTLVK 
EPVKDHRSE QTPPKVTNE WISVSSRKK RKNKNVEEI 
EDFEEVEAA PPSEEKVEP VVIIQPPPP PPPPPPPPE 
PEPVITVKK VKKKTKKVV APPPITKRV IIKDVDLSV 
EEVKEEPKP VVEEAPPPK KEEKVEEIV EKKESPEKK 
TKKKKKKVG KAATIVNSQ SSSNTTLNV TDDSYDFLM 
DESMEKTNV EVSQELDKL IQRGMYNNL EEKIKSMNI 
ESNDNFFKS IITNISSSR EPSVEKPYI KTPDLSKIL 
PNQALLKPR KPGDFLGES PTPSPLGGG SPPPSDNLY 
PITKAVKEW MSKTRESAP DIELLKSPS EIFREFTER 
EDDDDVTSD EDCRTVQAE QTGKYPKHG NLPYRAICC 
SIM
      CDS sequence
      • >TC015315 RA
        atgggaaag aagggtcgc aagaccagg tggcgcact 
ttaactatc acagatgag cacagcgat agtgaagaa 
tccaacacg acaacatcg ggacaccga ttacccaaa 
ggctaccca cacaagtca tactacagc cgttttacg 
tattctagt caatcaacg cccaccaga aggtaccag 
tgcgatggt acgaagtcg acaaggtcc agcagcact 
gccagtgaa aataaaatc accttcaat gaagacgaa 
tatacgcgc attacgaca cctagacaa gatgtatta 
tttaaaaag ggttacttg aataaaccc aaaacgtat 
caaacgcag acctcgacc ggaaattcg acgacttcg 
actggtaat tccaccgga aacggaaca ccagaccac 
caatcaaca gatctagaa tacgaatca cagttcgtc 
ttcccgaat ggtttcgtc gatcaaaac ggaatctac 
tatatcaac agttttgaa ccgtatcca gtgatggtt 
tacaatcct ccgacctat tatcaagaa tttacgaac 
attaaagcg aaaagatgc agcacagga tcgttgacg 
gaatcgacg agccccaac aacgaggaa actagtcaa 
agcgagaca cccgaagtg aatccagtc tacaatgtt 
gtttatccg aattattac tgtaacggc ggtgtttac 
gaggagccc cagaatggg gctatcagt gagcctgaa 
gcccgtcgt gtgaagaaa cgaagacgg cgaaagccg 
tcacggtcg aaagagggc accaccacg tctgaatct 
tccgatgaa ggagaagaa gaagaagag gaagaagaa 
gaaaaagaa gttttcagc gagaaagaa caacaaaaa 
acgtacgat ctcaaaccg gatgcggaa gagtttgtc 
cctcgggcc taccgccag ccccctgag accattccg 
atggaagca gccaacgtt caattcatc aaaattccg 
tcgaatttc gttccggtg ccgatcttg agcgatttt 
aacgcattt attccgcca gggattccg atcaacttt 
atacctcag aactttatc aattatgta cctttgaaa 
gcggccgtt caatcagac gatggtaaa gcagcgacg 
gacgacact gttaaagaa ctagcaacg gttgaagtt 
cctaaaaat caaatcgac attgcaaaa atcgtatcc 
aagctcgaa gaagcagcc aaagaacaa gacaaagat 
gaaacaaca ccgaaccat aaagcgaaa aagagacac 
cgataccag aaagttgaa ctgagaaaa ccaccacca 
agtcccgca gaaccgttc aaaccacca ctccgtaac 
tattccgaa actttgaag aaaattccg cttaaaacg 
aacgaacgt agcgaggaa aaaaaaaca ctggttaaa 
gaaccagtc aaggatcat cgaagtgaa caaacgcca 
ccgaaagtg acaaatgag tggatttcg gtgtcgagt 
cgtaaaaaa cgaaagaac aaaaatgtg gaagaaatt 
gaagatttt gaagaagtt gaagcagcg cccccttct 
gaagaaaaa gttgaaccg gttgttatt attcaacca 
ccacctcca ccaccacca ccaccacca ccaccggaa 
ccagagcca gttattaca gtgaagaaa gttaagaag 
aagacgaag aaagtggtg gctccgccc ccaattacc 
aaacgagtg attatcaag gacgttgat ttgtcggtg 
gaagaagtg aaggaggag cctaaaccg gttgtggag 
gaagcaccg ccccccaaa aaagaagaa aaggttgaa 
gaaattgtg gaaaagaag gaatctccc gaaaagaaa 
acgaagaag aagaagaag aaagttggc aaggctgct 
acgattgtg aatagccaa tccagctcg aatacgacg 
cttaatgtg acagatgat tcctatgat tttctcatg 
gatgagtcg atggagaag actaacgtt gaggtgtcg 
caagagctg gacaaactc atccagcgc ggaatgtac 
aacaatctc gaagagaaa atcaaatca atgaacatt 
gaatcaaac gacaacttc ttcaaaagc ataatcacc 
aacatatca agcagtcgg gagccaagt gtggaaaaa 
ccgtacata aaaaccccc gacttgagc aaaatcctc 
ccaaaccaa gcgctcctc aagccacga aaaccgggc 
gatttttta ggtgaaagc cccaccccc agcccactc 
gggggtggt tccccgccc cccagcgac aatctctac 
cccatcacg aaagccgtg aaggagtgg atgagcaag 
acgcgcgaa agcgccccc gacatcgag ttactcaaa 
agtccgagc gagattttt cgggaattc accgaaagg 
gaggacgac gatgacgta acaagcgac gaagattgc 
cgaacggtg caagccgag caaacgggg aaatacccc 
aaacacggc aacttgccc tacagggcg atttgctgc 
agtattatg tga
      mRNA sequence
      • >TC015315 RA
        ccgaaggcg tccggaggc gtccgatcg attacaaaa 
tagtgaaga agcgccggt ccggcgcag agtgtttta 
gtggctatg cggagacaa tttagtcga taacgagcg 
ctccgagtt acgtaatgt aatatcact ggagaattg 
tcaaaaaaa tcaaacatg ctgtagacc gaatggggt 
aacccccgg ttagtgact ggcgactgt ttcgggcac 
cacgcggga ataattaat aagaaacag tgcgaaaaa 
aataaattt gggtgagtg tctgatggt ggattaaat 
ccgttcgaa accggtaga aaggaaaat gggaaagaa 
gggtcgcaa gaccaggtg gcgcacttt aactatcac 
agatgagca cagcgatag tgaagaatc caacacgac 
aacatcggg acaccgatt acccaaagg ctacccaca 
caagtcata ctacagccg ttttacgta ttctagtca 
atcaacgcc caccagaag gtaccagtg cgatggtac 
gaagtcgac aaggtccag cagcactgc cagtgaaaa 
taaaatcac cttcaatga agacgaata tacgcgcat 
tacgacacc tagacaaga tgtattatt taaaaaggg 
ttacttgaa taaacccaa aacgtatca aacgcagac 
ctcgaccgg aaattcgac gacttcgac tggtaattc 
caccggaaa cggaacacc agaccacca atcaacaga 
tctagaata cgaatcaca gttcgtctt cccgaatgg 
tttcgtcga tcaaaacgg aatctacta tatcaacag 
ttttgaacc gtatccagt gatggttta caatcctcc 
gacctatta tcaagaatt tacgaacat taaagcgaa 
aagatgcag cacaggatc gttgacgga atcgacgag 
ccccaacaa cgaggaaac tagtcaaag cgagacacc 
cgaagtgaa tccagtcta caatgttgt ttatccgaa 
ttattactg taacggcgg tgtttacga ggagcccca 
gaatggggc tatcagtga gcctgaagc ccgtcgtgt 
gaagaaacg aagacggcg aaagccgtc acggtcgaa 
agagggcac caccacgtc tgaatcttc cgatgaagg 
agaagaaga agaagagga agaagaaga aaaagaagt 
tttcagcga gaaagaaca acaaaaaac gtacgatct 
caaaccgga tgcggaaga gtttgtccc tcgggccta 
ccgccagcc ccctgagac cattccgat ggaagcagc 
caacgttca attcatcaa aattccgtc gaatttcgt 
tccggtgcc gatcttgag cgattttaa cgcatttat 
tccgccagg gattccgat caactttat acctcagaa 
ctttatcaa ttatgtacc tttgaaagc ggccgttca 
atcagacga tggtaaagc agcgacgga cgacactgt 
taaagaact agcaacggt tgaagttcc taaaaatca 
aatcgacat tgcaaaaat cgtatccaa gctcgaaga 
agcagccaa agaacaaga caaagatga aacaacacc 
gaaccataa agcgaaaaa gagacaccg ataccagaa 
agttgaact gagaaaacc accaccaag tcccgcaga 
accgttcaa accaccact ccgtaacta ttccgaaac 
tttgaagaa aattccgct taaaacgaa cgaacgtag 
cgaggaaaa aaaaacact ggttaaaga accagtcaa 
ggatcatcg aagtgaaca aacgccacc gaaagtgac 
aaatgagtg gatttcggt gtcgagtcg taaaaaacg 
aaagaacaa aaatgtgga agaaattga agattttga 
agaagttga agcagcgcc cccttctga agaaaaagt 
tgaaccggt tgttattat tcaaccacc acctccacc 
accaccacc accaccacc accggaacc agagccagt 
tattacagt gaagaaagt taagaagaa gacgaagaa 
agtggtggc tccgccccc aattaccaa acgagtgat 
tatcaagga cgttgattt gtcggtgga agaagtgaa 
ggaggagcc taaaccggt tgtggagga agcaccgcc 
ccccaaaaa agaagaaaa ggttgaaga aattgtgga 
aaagaagga atctcccga aaagaaaac gaagaagaa 
gaagaagaa agttggcaa ggctgctac gattgtgaa 
tagccaatc cagctcgaa tacgacgct taatgtgac 
agatgattc ctatgattt tctcatgga tgagtcgat 
ggagaagac taacgttga ggtgtcgca agagctgga 
caaactcat ccagcgcgg aatgtacaa caatctcga 
agagaaaat caaatcaat gaacattga atcaaacga 
caacttctt caaaagcat aatcaccaa catatcaag 
cagtcggga gccaagtgt ggaaaaacc gtacataaa 
aacccccga cttgagcaa aatcctccc aaaccaagc 
gctcctcaa gccacgaaa accgggcga ttttttagg 
tgaaagccc cacccccag cccactcgg gggtggttc 
cccgccccc cagcgacaa tctctaccc catcacgaa 
agccgtgaa ggagtggat gagcaagac gcgcgaaag 
cgcccccga catcgagtt actcaaaag tccgagcga 
gatttttcg ggaattcac cgaaaggga ggacgacga 
tgacgtaac aagcgacga agattgccg aacggtgca 
agccgagca aacggggaa ataccccaa acacggcaa 
cttgcccta cagggcgat ttgctgcag tattatgtg 
atggcggct gacgcgatt acttaattc tggacgtac 
ttaagggct tgttctttt tatgtggcg cacacaacg 
gttggtaac actgtttcc aaggcctgc tgcttcaca 
cagagactg aatacgatg atgtttaag gcagggctt 
ggtgtcata tgttcgtga aatgttgtt attgttaaa 
tagttgtta ggggaagtc cggccgcgg tttaattat 
gtagcggcg acgatttta gttggttca tcatcaaag 
ccatttgct cattttatt ggttgttta ggatgtagt 
gtgcaattt ttatttttt tttttcaag tgtgggtgg 
gacacaccc ttaaagcgg cgacaatat tgttaaagg 
ggtgtgaat ttttcaaca aaaaagaca ctatgtatg 
ctaagtcta gtgttaagt ttgttttgc gcatgtatg 
gagtttttt tcttaagtt tttctttcg tttggttgt 
tctatatca gttatttta ttgttataa tgtttgtta 
tttttgaga gttgtgcct ttgcaaaat ttttaaaat 
aaagaaaat tataataa
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY