General information | |||
TC015315 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007421.3: 584442..600689 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC015315 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_02451 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
larva random constricted - Penetrance (percentage of animals/eggs on slide): less than 30%
procephalic head irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
- >TC015315 RA
MGKKGRKTR WRTLTITDE HSDSEESNT TTSGHRLPK GYPHKSYYS RFTYSSQST PTRRYQCDG TKSTRSSST ASENKITFN EDEYTRITT PRQDVLFKK GYLNKPKTY QTQTSTGNS TTSTGNSTG NGTPDHQST DLEYESQFV FPNGFVDQN GIYYINSFE PYPVMVYNP PTYYQEFTN IKAKRCSTG SLTESTSPN NEETSQSET PEVNPVYNV VYPNYYCNG GVYEEPQNG AISEPEARR VKKRRRRKP SRSKEGTTT SESSDEGEE EEEEEEEKE VFSEKEQQK TYDLKPDAE EFVPRAYRQ PPETIPMEA ANVQFIKIP SNFVPVPIL SDFNAFIPP GIPINFIPQ NFINYVPLK AAVQSDDGK AATDDTVKE LATVEVPKN QIDIAKIVS KLEEAAKEQ DKDETTPNH KAKKRHRYQ KVELRKPPP SPAEPFKPP LRNYSETLK KIPLKTNER SEEKKTLVK EPVKDHRSE QTPPKVTNE WISVSSRKK RKNKNVEEI EDFEEVEAA PPSEEKVEP VVIIQPPPP PPPPPPPPE PEPVITVKK VKKKTKKVV APPPITKRV IIKDVDLSV EEVKEEPKP VVEEAPPPK KEEKVEEIV EKKESPEKK TKKKKKKVG KAATIVNSQ SSSNTTLNV TDDSYDFLM DESMEKTNV EVSQELDKL IQRGMYNNL EEKIKSMNI ESNDNFFKS IITNISSSR EPSVEKPYI KTPDLSKIL PNQALLKPR KPGDFLGES PTPSPLGGG SPPPSDNLY PITKAVKEW MSKTRESAP DIELLKSPS EIFREFTER EDDDDVTSD EDCRTVQAE QTGKYPKHG NLPYRAICC SIM
- >TC015315 RA
atgggaaag aagggtcgc aagaccagg tggcgcact ttaactatc acagatgag cacagcgat agtgaagaa tccaacacg acaacatcg ggacaccga ttacccaaa ggctaccca cacaagtca tactacagc cgttttacg tattctagt caatcaacg cccaccaga aggtaccag tgcgatggt acgaagtcg acaaggtcc agcagcact gccagtgaa aataaaatc accttcaat gaagacgaa tatacgcgc attacgaca cctagacaa gatgtatta tttaaaaag ggttacttg aataaaccc aaaacgtat caaacgcag acctcgacc ggaaattcg acgacttcg actggtaat tccaccgga aacggaaca ccagaccac caatcaaca gatctagaa tacgaatca cagttcgtc ttcccgaat ggtttcgtc gatcaaaac ggaatctac tatatcaac agttttgaa ccgtatcca gtgatggtt tacaatcct ccgacctat tatcaagaa tttacgaac attaaagcg aaaagatgc agcacagga tcgttgacg gaatcgacg agccccaac aacgaggaa actagtcaa agcgagaca cccgaagtg aatccagtc tacaatgtt gtttatccg aattattac tgtaacggc ggtgtttac gaggagccc cagaatggg gctatcagt gagcctgaa gcccgtcgt gtgaagaaa cgaagacgg cgaaagccg tcacggtcg aaagagggc accaccacg tctgaatct tccgatgaa ggagaagaa gaagaagag gaagaagaa gaaaaagaa gttttcagc gagaaagaa caacaaaaa acgtacgat ctcaaaccg gatgcggaa gagtttgtc cctcgggcc taccgccag ccccctgag accattccg atggaagca gccaacgtt caattcatc aaaattccg tcgaatttc gttccggtg ccgatcttg agcgatttt aacgcattt attccgcca gggattccg atcaacttt atacctcag aactttatc aattatgta cctttgaaa gcggccgtt caatcagac gatggtaaa gcagcgacg gacgacact gttaaagaa ctagcaacg gttgaagtt cctaaaaat caaatcgac attgcaaaa atcgtatcc aagctcgaa gaagcagcc aaagaacaa gacaaagat gaaacaaca ccgaaccat aaagcgaaa aagagacac cgataccag aaagttgaa ctgagaaaa ccaccacca agtcccgca gaaccgttc aaaccacca ctccgtaac tattccgaa actttgaag aaaattccg cttaaaacg aacgaacgt agcgaggaa aaaaaaaca ctggttaaa gaaccagtc aaggatcat cgaagtgaa caaacgcca ccgaaagtg acaaatgag tggatttcg gtgtcgagt cgtaaaaaa cgaaagaac aaaaatgtg gaagaaatt gaagatttt gaagaagtt gaagcagcg cccccttct gaagaaaaa gttgaaccg gttgttatt attcaacca ccacctcca ccaccacca ccaccacca ccaccggaa ccagagcca gttattaca gtgaagaaa gttaagaag aagacgaag aaagtggtg gctccgccc ccaattacc aaacgagtg attatcaag gacgttgat ttgtcggtg gaagaagtg aaggaggag cctaaaccg gttgtggag gaagcaccg ccccccaaa aaagaagaa aaggttgaa gaaattgtg gaaaagaag gaatctccc gaaaagaaa acgaagaag aagaagaag aaagttggc aaggctgct acgattgtg aatagccaa tccagctcg aatacgacg cttaatgtg acagatgat tcctatgat tttctcatg gatgagtcg atggagaag actaacgtt gaggtgtcg caagagctg gacaaactc atccagcgc ggaatgtac aacaatctc gaagagaaa atcaaatca atgaacatt gaatcaaac gacaacttc ttcaaaagc ataatcacc aacatatca agcagtcgg gagccaagt gtggaaaaa ccgtacata aaaaccccc gacttgagc aaaatcctc ccaaaccaa gcgctcctc aagccacga aaaccgggc gatttttta ggtgaaagc cccaccccc agcccactc gggggtggt tccccgccc cccagcgac aatctctac cccatcacg aaagccgtg aaggagtgg atgagcaag acgcgcgaa agcgccccc gacatcgag ttactcaaa agtccgagc gagattttt cgggaattc accgaaagg gaggacgac gatgacgta acaagcgac gaagattgc cgaacggtg caagccgag caaacgggg aaatacccc aaacacggc aacttgccc tacagggcg atttgctgc agtattatg tga
- >TC015315 RA
ccgaaggcg tccggaggc gtccgatcg attacaaaa tagtgaaga agcgccggt ccggcgcag agtgtttta gtggctatg cggagacaa tttagtcga taacgagcg ctccgagtt acgtaatgt aatatcact ggagaattg tcaaaaaaa tcaaacatg ctgtagacc gaatggggt aacccccgg ttagtgact ggcgactgt ttcgggcac cacgcggga ataattaat aagaaacag tgcgaaaaa aataaattt gggtgagtg tctgatggt ggattaaat ccgttcgaa accggtaga aaggaaaat gggaaagaa gggtcgcaa gaccaggtg gcgcacttt aactatcac agatgagca cagcgatag tgaagaatc caacacgac aacatcggg acaccgatt acccaaagg ctacccaca caagtcata ctacagccg ttttacgta ttctagtca atcaacgcc caccagaag gtaccagtg cgatggtac gaagtcgac aaggtccag cagcactgc cagtgaaaa taaaatcac cttcaatga agacgaata tacgcgcat tacgacacc tagacaaga tgtattatt taaaaaggg ttacttgaa taaacccaa aacgtatca aacgcagac ctcgaccgg aaattcgac gacttcgac tggtaattc caccggaaa cggaacacc agaccacca atcaacaga tctagaata cgaatcaca gttcgtctt cccgaatgg tttcgtcga tcaaaacgg aatctacta tatcaacag ttttgaacc gtatccagt gatggttta caatcctcc gacctatta tcaagaatt tacgaacat taaagcgaa aagatgcag cacaggatc gttgacgga atcgacgag ccccaacaa cgaggaaac tagtcaaag cgagacacc cgaagtgaa tccagtcta caatgttgt ttatccgaa ttattactg taacggcgg tgtttacga ggagcccca gaatggggc tatcagtga gcctgaagc ccgtcgtgt gaagaaacg aagacggcg aaagccgtc acggtcgaa agagggcac caccacgtc tgaatcttc cgatgaagg agaagaaga agaagagga agaagaaga aaaagaagt tttcagcga gaaagaaca acaaaaaac gtacgatct caaaccgga tgcggaaga gtttgtccc tcgggccta ccgccagcc ccctgagac cattccgat ggaagcagc caacgttca attcatcaa aattccgtc gaatttcgt tccggtgcc gatcttgag cgattttaa cgcatttat tccgccagg gattccgat caactttat acctcagaa ctttatcaa ttatgtacc tttgaaagc ggccgttca atcagacga tggtaaagc agcgacgga cgacactgt taaagaact agcaacggt tgaagttcc taaaaatca aatcgacat tgcaaaaat cgtatccaa gctcgaaga agcagccaa agaacaaga caaagatga aacaacacc gaaccataa agcgaaaaa gagacaccg ataccagaa agttgaact gagaaaacc accaccaag tcccgcaga accgttcaa accaccact ccgtaacta ttccgaaac tttgaagaa aattccgct taaaacgaa cgaacgtag cgaggaaaa aaaaacact ggttaaaga accagtcaa ggatcatcg aagtgaaca aacgccacc gaaagtgac aaatgagtg gatttcggt gtcgagtcg taaaaaacg aaagaacaa aaatgtgga agaaattga agattttga agaagttga agcagcgcc cccttctga agaaaaagt tgaaccggt tgttattat tcaaccacc acctccacc accaccacc accaccacc accggaacc agagccagt tattacagt gaagaaagt taagaagaa gacgaagaa agtggtggc tccgccccc aattaccaa acgagtgat tatcaagga cgttgattt gtcggtgga agaagtgaa ggaggagcc taaaccggt tgtggagga agcaccgcc ccccaaaaa agaagaaaa ggttgaaga aattgtgga aaagaagga atctcccga aaagaaaac gaagaagaa gaagaagaa agttggcaa ggctgctac gattgtgaa tagccaatc cagctcgaa tacgacgct taatgtgac agatgattc ctatgattt tctcatgga tgagtcgat ggagaagac taacgttga ggtgtcgca agagctgga caaactcat ccagcgcgg aatgtacaa caatctcga agagaaaat caaatcaat gaacattga atcaaacga caacttctt caaaagcat aatcaccaa catatcaag cagtcggga gccaagtgt ggaaaaacc gtacataaa aacccccga cttgagcaa aatcctccc aaaccaagc gctcctcaa gccacgaaa accgggcga ttttttagg tgaaagccc cacccccag cccactcgg gggtggttc cccgccccc cagcgacaa tctctaccc catcacgaa agccgtgaa ggagtggat gagcaagac gcgcgaaag cgcccccga catcgagtt actcaaaag tccgagcga gatttttcg ggaattcac cgaaaggga ggacgacga tgacgtaac aagcgacga agattgccg aacggtgca agccgagca aacggggaa ataccccaa acacggcaa cttgcccta cagggcgat ttgctgcag tattatgtg atggcggct gacgcgatt acttaattc tggacgtac ttaagggct tgttctttt tatgtggcg cacacaacg gttggtaac actgtttcc aaggcctgc tgcttcaca cagagactg aatacgatg atgtttaag gcagggctt ggtgtcata tgttcgtga aatgttgtt attgttaaa tagttgtta ggggaagtc cggccgcgg tttaattat gtagcggcg acgatttta gttggttca tcatcaaag ccatttgct cattttatt ggttgttta ggatgtagt gtgcaattt ttatttttt tttttcaag tgtgggtgg gacacaccc ttaaagcgg cgacaatat tgttaaagg ggtgtgaat ttttcaaca aaaaagaca ctatgtatg ctaagtcta gtgttaagt ttgttttgc gcatgtatg gagtttttt tcttaagtt tttctttcg tttggttgt tctatatca gttatttta ttgttataa tgtttgtta tttttgaga gttgtgcct ttgcaaaat ttttaaaat aaagaaaat tataataa
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
