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Gene information
General information
TC015310 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid39: 645485..649443
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015310
    iBeetle screen iB_02448
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    egg chamber size irregular - Number of animals showing the phenotype: 3

    egg chamber shape irregular - Number of animals showing the phenotype: 3
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015310 RA
      MASVQTTVL LVLGVFIEI CLAQRSPTI SYITQTQIK 
      DIGGTVELS CSVQYTNDY PVIWMKIDP RSNANSLPI 
      STGSTLILH ESRYSLRYD EASSTYTLQ IKDIQETDA 
      GFYHCQIII SPSNRVSAE VELQVRRPP FISDNSTRS 
      VVVSEGQAV QMECYAGGY PPPRISWRR ENNAILPTG 
      GSIYRGNVM KIKQIKKED RGTYYCVAE NGVGRGTKR 
      NIAVEVEFA PVVTVPRPR LGQALQYDM DLECHVEAY 
      PPPALTWVK DEVALSNNQ HYSISHFAT ADEFTDTTL 
      RVITIEKRQ YGQYICKAS NKLGSAEGV VELFESIIP 
      VCPPACGQA RYGDAATLS TSTAAICLT LLFVLFRDF 
      HAQH
    CDS sequence
    • >TC015310 RA
      atggcgtcg gtgcaaaca acagttttg ttagtttta 
      ggtgttttt atagaaatt tgtttggca caaaggtcc 
      cccacgata tcgtacata actcaaact cagattaag 
      gatattggg ggcacagtg gaattatca tgttcggtt 
      caatacacg aacgattat ccagtgatc tggatgaag 
      atcgatccc aggtcgaac gccaacagt ttgcccatt 
      tccaccgga agtacgcta attctgcac gaatcccga 
      tattcgctg cgttatgac gaggcgagt tcgacttat 
      acgctacaa attaaagac attcaagaa accgacgct 
      ggtttctac cattgtcaa atcataatt tcgccaagt 
      aatagggtc tcagctgag gtggaatta caagtacgg 
      cgcccccct ttcatttcc gacaattcg acgcgatcg 
      gttgtcgta tcagaggga caagccgtc cagatggag 
      tgctacgcc gggggttac ccgccgccg agaatctca 
      tggcgtcgt gaaaacaac gccattttg cccactggt 
      ggctccatc taccgtggc aacgtgatg aaaatcaaa 
      caaataaaa aaagaagat cgtggcaca tattattgc 
      gtcgctgag aatggcgtt ggccgaggg acgaaacgt 
      aacattgcc gtcgaagtg gaatttgcg ccggttgtg 
      accgtgcct cggccacgc ctcggtcaa gccctccaa 
      tacgacatg gatttggag tgtcacgtg gaggcgtat 
      cccccgcct gctttgacc tgggtcaag gacgaagtc 
      gcattatcc aataaccaa cattacagt atttcgcat 
      ttcgcaaca gcggacgaa ttcaccgac acgacttta 
      cgtgtgata acgattgag aaacgccag tatgggcag 
      tatatttgt aaagcgtcg aacaaattg ggctcggca 
      gagggcgtg gtcgagttg tttgagtcc atcattcct 
      gtgtgtcct cctgcttgc ggtcaggca aggtatggt 
      gatgcggcc acattgtcg acaagtacc gcagctata 
      tgtttgacg ttactgttc gtcttgttt cgagatttc 
      cacgcccaa cattaa
    mRNA sequence
    • >TC015310 RA
      ggggtctcg ggcccctaa actcaagca aaccgttca 
      atttcccgg taatttcac aataccggt tgagtttgg 
      ggcagtttc agtcaagtt ttggcgaaa tttggccca 
      aaacttggt aaatgggcg cgccgccac tgactttga 
      gcaggtgtg gtgaccaag atggcgtcg gtgcaaaca 
      acagttttg ttagtttta ggtgttttt atagaaatt 
      tgtttggca caaaggtcc cccacgata tcgtacata 
      actcaaact cagattaag gatattggg ggcacagtg 
      gaattatca tgttcggtt caatacacg aacgattat 
      ccagtgatc tggatgaag atcgatccc aggtcgaac 
      gccaacagt ttgcccatt tccaccgga agtacgcta 
      attctgcac gaatcccga tattcgctg cgttatgac 
      gaggcgagt tcgacttat acgctacaa attaaagac 
      attcaagaa accgacgct ggtttctac cattgtcaa 
      atcataatt tcgccaagt aatagggtc tcagctgag 
      gtggaatta caagtacgg cgcccccct ttcatttcc 
      gacaattcg acgcgatcg gttgtcgta tcagaggga 
      caagccgtc cagatggag tgctacgcc gggggttac 
      ccgccgccg agaatctca tggcgtcgt gaaaacaac 
      gccattttg cccactggt ggctccatc taccgtggc 
      aacgtgatg aaaatcaaa caaataaaa aaagaagat 
      cgtggcaca tattattgc gtcgctgag aatggcgtt 
      ggccgaggg acgaaacgt aacattgcc gtcgaagtg 
      gaatttgcg ccggttgtg accgtgcct cggccacgc 
      ctcggtcaa gccctccaa tacgacatg gatttggag 
      tgtcacgtg gaggcgtat cccccgcct gctttgacc 
      tgggtcaag gacgaagtc gcattatcc aataaccaa 
      cattacagt atttcgcat ttcgcaaca gcggacgaa 
      ttcaccgac acgacttta cgtgtgata acgattgag 
      aaacgccag tatgggcag tatatttgt aaagcgtcg 
      aacaaattg ggctcggca gagggcgtg gtcgagttg 
      tttgagtcc atcattcct gtgtgtcct cctgcttgc 
      ggtcaggca aggtatggt gatgcggcc acattgtcg 
      acaagtacc gcagctata tgtttgacg ttactgttc 
      gtcttgttt cgagatttc cacgcccaa cattaatta 
      tccaggcct tgtgtggtt gaatacgag tccaaggcc 
      aacttgcaa cgccgtctt aattacgat tacaattct 
      cacaacgac tctgttgca tttcttttg tttgtttta 
      gtgagtttt attagaaag gaccaaaaa acagtgaca 
      gcaatattt aatcgatag tgttaaggt tttttacac 
      gactttttg atacagtta attccattt tttatgtat 
      atttttgac acgctctgt atatttttt gaaattctt 
      ttacaagat attgtaaat gatttattt ttttaattt 
      aattttttt tatagcacg gttgtgcgt agtctaagg 
      cctaattgt ttcttgcta gcaacagct ctagtcaac 
      aaatgacag ctgtttttg gcaacaacg tcaaatttt 
      atttgtcca attcgtcca ttaaattaa tttttttgt 
      caaagttgt ttctttaaa gaatttttt ttaatttcc 
      cgcaaaaaa actccaata aaccgatta aaaaccg