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Gene information
General information
TC015301 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid39: 765015..769619
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015301
    iBeetle screen iB_02447
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    larva posterior partiallynot present -

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015301 RA
      MDSKRKLST QGDSLYQIL ALPKTATSE 
      EIKKTYRRL ALKYHPDKN PNNPEASEK 
      FKEVNRAHS ILSDPTKRN IYDNYGSLG 
      LYIAEQFGE ENVNAYFVV TSTWCKALF 
      VVCGILSCC YCCCCLCCC CNFCCGKCK 
      PRPPEETGD YHTLNREQG PSNEPVTIQ 
      PGKREDGSD DELAGSPKN DSATITTQP 
      SPKKGPNAP VYAMPPPTS NLGESAALN 
      QGDVPVYTP GMTNSPTKS QQW
    CDS sequence
    • >TC015301 RA
      atggattca aaacgcaaa ctatccaca cagggggat 
      tccctgtac cagatctta gcactgccc aagactgcg 
      acgtcggaa gagatcaag aagacctac agacggcta 
      gcgctcaag taccatcca gataagaat cccaacaat 
      ccggaagcg tccgagaag tttaaggaa gtgaacagg 
      gcacactcg attctgagt gatccgacg aaaaggaac 
      atttatgat aattacggc tcgttgggg ctctacatt 
      gccgaacag tttggggaa gagaatgtc aatgcgtat 
      tttgttgtt acgtcgacc tggtgcaag gccctgttc 
      gtcgtttgt ggtatccta tcgtgttgt tactgctgt 
      tgttgtttg tgttgttgt tgcaatttc tgttgcggc 
      aaatgcaaa ccacgacca ccggaagaa accggcgat 
      tatcacacg ttgaatcga gaacaaggt ccaagtaat 
      gaacctgtc acgatccag ccaggtaaa cgggaggac 
      ggctctgat gatgaattg gccggttcg cctaaaaac 
      gattcggca acgatcaca acacaaccg tctcccaaa 
      aagggcccc aatgccccc gtttacgcc atgccgccg 
      ccaacgtcc aatttgggc gaaagcgcg gccctaaat 
      cagggtgac gtgcccgtc tacacgcca ggaatgacg 
      aattcgcca acgaaaagc cagcaatgg tga
    mRNA sequence
    • >TC015301 RA
      cacgtcagc ttgaccacc gtctgccgt catcgaacg 
      gtttttatt gacaattgc tggtcgtgt tgtaaaaat 
      cgtgcaaaa tgcggcttt agatcaaag tatttccta 
      ttctcccgc ttccagagt cgattttga gcgttgtat 
      gtgcgttaa ggttgggaa aatcgccac ggaatggat 
      tcaaaacgc aaactatcc acacagggg gattccctg 
      taccagatc ttagcactg cccaagact gcgacgtcg 
      gaagagatc aagaagacc tacagacgg ctagcgctc 
      aagtaccat ccagataag aatcccaac aatccggaa 
      gcgtccgag aagtttaag gaagtgaac agggcacac 
      tcgattctg agtgatccg acgaaaagg aacatttat 
      gataattac ggctcgttg gggctctac attgccgaa 
      cagtttggg gaagagaat gtcaatgcg tattttgtt 
      gttacgtcg acctggtgc aaggccctg ttcgtcgtt 
      tgtggtatc ctatcgtgt tgttactgc tgttgttgt 
      ttgtgttgt tgttgcaat ttctgttgc ggcaaatgc 
      aaaccacga ccaccggaa gaaaccggc gattatcac 
      acgttgaat cgagaacaa ggtccaagt aatgaacct 
      gtcacgatc cagccaggt aaacgggag gacggctct 
      gatgatgaa ttggccggt tcgcctaaa aacgattcg 
      gcaacgatc acaacacaa ccgtctccc aaaaagggc 
      cccaatgcc cccgtttac gccatgccg ccgccaacg 
      tccaatttg ggcgaaagc gcggcccta aatcagggt 
      gacgtgccc gtctacacg ccaggaatg acgaattcg 
      ccaacgaaa agccagcaa tggtgaggg gagagagtc 
      gatgttaac aaccaccaa gctcttgaa tcctctagt 
      catgagaaa taataaaaa cgcgactta aattcgcct 
      gaatttaca cttaaaaaa gtatttttt gtctccttg 
      tcttaccat ttatcgaca cacatttaa aactattag 
      ccaagttta aaataataa atgagagta cgaattttt 
      tgtagcaaa actcgtgtc atgtttgtt agcacgtaa 
      aggggttat atttacgtc ctttttatt gttttattt 
      tttcgtttg tttatttat tgaggactc gatgagttc 
      tagtattgt atgctactt ttggcagtt tgttgtaaa 
      aggggtatt tcattactt gctgttagt gttaaattc 
      gagttttga atgattatt atcgattag gtaagttgt 
      gaggacacc tgtttgtaa aaataataa ttgcatcac 
      tagttaaaa tctgtaatt ttactattt atgaatgta 
      taattgtta aacaaacga ttattttag cttagatat 
      tatacagca acttgaatg tacattatc aattaaatt 
      gcgtaacaa caatttttt gctttcatt tctcccaag 
      tcagtcagt gtttattat taaactgta taataataa 
      taaaaaacg tagtcacgt taccgatta tttgcagta 
      atgttgttt tattaatgg agaaaaaac cagccgccg 
      aaaaatcgg gataattat tcaaaacca cattaataa 
      attcggaaa tatttttct tctcgtttg gaaacactt 
      gtcgaattt tataagcca acaaaagtg tagttttta 
      cttcctcca aaaccgcat cacacgata atatcaaag 
      ttacggtac caacctaaa aattaaaac cggtatttt 
      ccaaaaaaa caaattccc ccaactcac atttatcat 
      aaatgcaac gccaacgtt tgcaacaac cactccaat 
      gtattgttt tttgtcgta accgaaatt attattaaa 
      aacctcatc aatatagtt caagaccat ca