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Gene information
General information
TC015284 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid39: 945339..947993
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015284
    iBeetle screen iB_02445
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015284 RA
      MKPKILILI LTSALIAKS EKYFNENDI PELTYLDPS 
      TNPCDNFYQ FTCGNFAKI HPRPSTLNV LDHFTLLEN 
      ELIQVGQEI LSSPVKEND PKAVKKAKT AYKSCVQSQ 
      VLNNVVPEL RTVKEFGGM PLLSSSNNQ TISFSWNEV 
      GKLVSEYGV PLIFVYSVA QKSQNESMI VLSWDTLNN 
      PTLFRQVLQ KTYDHVLEE DFAKFGTSR VTRSTSPQP 
      FDIFLETVA KNLLKYTNP EKPEGEVLD ELDEMATFM 
      RTLYKGGIK GSTILPLPS EVTLGQLQE WTYQQFGEA 
      VIDWVQYLG LVFQKSGVK ITPMTKLYH QDIERIYGI 
      LNLVKKTDP KVLKNFALL RVFLFQAPD SGSATRQAF 
      EKYYQAKNN QLYPRWEYC TRKILDVVD TASLSYAVT 
      YNYLQYHFS TEKLAQVTQ MIENIREVF SETLNASDW 
      MDEESREQA LKKATNMLV LLGYPDFVQ DQDTLDQFY 
      AKFRVCERD NYGNARTIR AFKQAYQFT QLGKLDRLF 
      WGKSAFDAN AYYNRPNNK IVVPLSMLN PVFFHGGNP 
      VVDYSRLGS IIGHEMTHG FDVNGKNYD QDGTLSTWW 
      SQKTTDAFN ERSECFKKQ YSQYYVPEI GLYVNGSYC 
      INENLADNG GIRQSYKAF ESLAKAQKI KSNGNYTLE 
      QLFFIGYGT MWCSDESNY ALSVIVNTV NGYPPKRYR 
      VIGSLANMV EFAEAFNCP VGSTMNPEE KCQLW
    CDS sequence
    • >TC015284 RA
      atgaagcca aaaatccta atcctaatt ctgaccagt 
      gccctaatt gccaaatcc gaaaagtac ttcaacgaa 
      aatgacatt ccagagctg acctacttg gacccatca 
      actaaccca tgcgacaac ttctaccag ttcacttgc 
      ggaaacttc gccaaaatc cacccaaga ccatcaacc 
      ctcaacgtc cttgaccac ttcactctc ctcgaaaac 
      gagctgatt caagttggg caagaaatt ttatcgagt 
      cctgtcaaa gaaaacgac ccaaaagcg gtaaaaaaa 
      gccaaaacg gcttacaaa agctgcgtc cagtcgcaa 
      gttttaaat aatgtagta ccagaacta cgtactgtt 
      aaagaattt ggtggaatg ccactcctc tcctcttct 
      aataatcaa actataagt ttttcctgg aacgaagtc 
      ggaaagttg gttagtgag tacggggtt cccctaatt 
      tttgtttac agcgtggcg cagaaaagc caaaatgag 
      agcatgata gtactaagt tgggacact ctaaacaac 
      ccgactttg ttccgacaa gttttgcaa aaaacctac 
      gatcatgtc ttggaagaa gatttcgca aaatttgga 
      accagtcga gtcactcga tcgactagt cctcaaccg 
      tttgatatt tttctcgaa actgtggcc aaaaatttg 
      ctaaaatac accaatccg gagaagcct gaaggggaa 
      gtactggac gaacttgac gaaatggca acttttatg 
      agaacactt tacaaagga ggaataaaa ggtagtacc 
      attttgcca cttccttca gaagtgact ttggggcaa 
      ctccaagag tggacgtac caacaattt ggggaagca 
      gttatagac tgggtgcag taccttggc ctagtgttc 
      caaaaatcg ggcgtcaaa atcacccca atgacaaaa 
      ctgtaccac caggacatt gaacgcatt tacggcatt 
      ttgaacttg gtcaaaaaa accgacccc aaagtactc 
      aaaaacttt gccttgtta cgtgttttc ctcttccaa 
      gcgcctgac agtggctcc gccacacgt caagccttt 
      gaaaagtac taccaagcg aaaaacaac caactgtac 
      cccagatgg gagtactgt acacgaaaa atcctcgat 
      gttgttgat acagccagt ctgagttat gctgtgact 
      tataactac ctccagtac cactttagt accgaaaaa 
      ttggcccag gttacacaa atgatcgaa aatatacga 
      gaagttttc agcgaaacg ttgaatgcg tctgattgg 
      atggacgaa gagtcacgt gagcaagct ttgaaaaaa 
      gccaccaac atgttggta ctacttggc taccctgat 
      tttgtccaa gaccaagac acacttgat cagttttac 
      gcaaagttt cgggtttgt gaacgagat aattacgga 
      aacgctcga acgattcgt gcgtttaag caggcgtac 
      caattcaca cagttgggg aaacttgac cgtttattt 
      tgggggaaa tcagccttc gatgctaat gcttactac 
      aaccgtccg aacaacaaa attgtggta ccactttca 
      atgctcaac ccggtgttt ttccatggt ggtaaccca 
      gttgtggac tacagccgt cttggtagt ataatcggg 
      cacgaaatg acccatggt ttcgatgtc aatggtaaa 
      aactacgac caagatggt actttatcg acatggtgg 
      tcccaaaaa accacggat gcgtttaat gaacgatcc 
      gaatgtttc aaaaaacag tacagccaa tattacgtc 
      ccggaaatt ggtctttac gtgaatggt tcctactgt 
      attaatgaa aatttggca gacaatggt ggcattaga 
      caaagttac aaagcgttt gagtcgttg gcaaaagcc 
      caaaaaatc aaatctaac ggtaactac accctggaa 
      caactcttc ttcattggt tatggaact atgtggtgc 
      agtgacgaa tcaaattac gccttatca gtgattgtt 
      aatacagtt aatgggtat ccaccgaag aggtatcgc 
      gttattggt agtttagca aatatggtg gaatttgcg 
      gaagctttt aactgtccg gttggtagt accatgaac 
      ccggaagag aagtgccag ttgtggtga
    mRNA sequence
    • >TC015284 RA
      cacgttaat ttttaatag ataaacaaa cgaaaactt 
      tattaccaa aaactcaaa ccaaaccaa gcaaagtgc 
      aatgaagcc aaaaatcct aatcctaat tctgaccag 
      tgccctaat tgccaaatc cgaaaagta cttcaacga 
      aaatgacat tccagagct gacctactt ggacccatc 
      aactaaccc atgcgacaa cttctacca gttcacttg 
      cggaaactt cgccaaaat ccacccaag accatcaac 
      cctcaacgt ccttgacca cttcactct cctcgaaaa 
      cgagctgat tcaagttgg gcaagaaat tttatcgag 
      tcctgtcaa agaaaacga cccaaaagc ggtaaaaaa 
      agccaaaac ggcttacaa aagctgcgt ccagtcgca 
      agttttaaa taatgtagt accagaact acgtactgt 
      taaagaatt tggtggaat gccactcct ctcctcttc 
      taataatca aactataag tttttcctg gaacgaagt 
      cggaaagtt ggttagtga gtacggggt tcccctaat 
      ttttgttta cagcgtggc gcagaaaag ccaaaatga 
      gagcatgat agtactaag ttgggacac tctaaacaa 
      cccgacttt gttccgaca agttttgca aaaaaccta 
      cgatcatgt cttggaaga agatttcgc aaaatttgg 
      aaccagtcg agtcactcg atcgactag tcctcaacc 
      gtttgatat ttttctcga aactgtggc caaaaattt 
      gctaaaata caccaatcc ggagaagcc tgaagggga 
      agtactgga cgaacttga cgaaatggc aacttttat 
      gagaacact ttacaaagg aggaataaa aggtagtac 
      cattttgcc acttccttc agaagtgac tttggggca 
      actccaaga gtggacgta ccaacaatt tggggaagc 
      agttataga ctgggtgca gtaccttgg cctagtgtt 
      ccaaaaatc gggcgtcaa aatcacccc aatgacaaa 
      actgtacca ccaggacat tgaacgcat ttacggcat 
      tttgaactt ggtcaaaaa aaccgaccc caaagtact 
      caaaaactt tgccttgtt acgtgtttt cctcttcca 
      agcgcctga cagtggctc cgccacacg tcaagcctt 
      tgaaaagta ctaccaagc gaaaaacaa ccaactgta 
      ccccagatg ggagtactg tacacgaaa aatcctcga 
      tgttgttga tacagccag tctgagtta tgctgtgac 
      ttataacta cctccagta ccactttag taccgaaaa 
      attggccca ggttacaca aatgatcga aaatatacg 
      agaagtttt cagcgaaac gttgaatgc gtctgattg 
      gatggacga agagtcacg tgagcaagc tttgaaaaa 
      agccaccaa catgttggt actacttgg ctaccctga 
      ttttgtcca agaccaaga cacacttga tcagtttta 
      cgcaaagtt tcgggtttg tgaacgaga taattacgg 
      aaacgctcg aacgattcg tgcgtttaa gcaggcgta 
      ccaattcac acagttggg gaaacttga ccgtttatt 
      ttgggggaa atcagcctt cgatgctaa tgcttacta 
      caaccgtcc gaacaacaa aattgtggt accactttc 
      aatgctcaa cccggtgtt tttccatgg tggtaaccc 
      agttgtgga ctacagccg tcttggtag tataatcgg 
      gcacgaaat gacccatgg tttcgatgt caatggtaa 
      aaactacga ccaagatgg tactttatc gacatggtg 
      gtcccaaaa aaccacgga tgcgtttaa tgaacgatc 
      cgaatgttt caaaaaaca gtacagcca atattacgt 
      cccggaaat tggtcttta cgtgaatgg ttcctactg 
      tattaatga aaatttggc agacaatgg tggcattag 
      acaaagtta caaagcgtt tgagtcgtt ggcaaaagc 
      ccaaaaaat caaatctaa cggtaacta caccctgga 
      acaactctt cttcattgg ttatggaac tatgtggtg 
      cagtgacga atcaaatta cgccttatc agtgattgt 
      taatacagt taatgggta tccaccgaa gaggtatcg 
      cgttattgg tagtttagc aaatatggt ggaatttgc 
      ggaagcttt taactgtcc ggttggtag taccatgaa 
      cccggaaga gaagtgcca gttgtggtg aataataaa 
      atgtttgct ttgat