Status
Please wait ...
Gene information
General information
TC030896
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC015278,TC015279,TC015277,TC015276
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 963759..1027251
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030896
    iBeetle screen iB_02444

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030896 RA
      MFNFIKKAG TGVTSDKDE KEKRKRDKK DRKDKQKRE 
      RASMSAEEL LRLDEVRRS LKIRGRRKE KEKLPSGIT 
      ADYTADFLA SLDRQESTV PHMNHLQEG HTQSDSSET 
      SLNSLNNPQ TNKVLPPLP PKPPKRGIL KTPRVSVTQ 
      DNGYDNLVR NTIQNELIT YQNIPNGGI NGKNLGSEL 
      LVITSTSPS ADSLTDTTN SSFATPPFS LSPVGESQG 
      FHRWSRTSN FDDVELPLP EIAPLTLPK PRTLTIQRQ 
      KPPRNDFGF SLRRAMVLE RVVVNGNGA AGDGTLTMK 
      AVIFAEPGA MVQHNNETG LLPGDKLLE VNGFPVEDK 
      TREEIIDKI KASGECVTL KVQPVAELS ELSRRSAHD 
      GGEVVLDDS NIRGGTLRR SGSRRFNKS TTAKSEEHL 
      EKEKQWLDT EKVWLMHRG GFTAALKNG ENFTEPGKI 
      QVCLEHSKE ILTVDEDDI EKANPPQFD RAEDLAALR 
      HLNESSILH TLRQRYATN LIHTYAGCG TLLVINPMA 
      PVAIYSEKV VALFKGCKS EDMPPHIYS MAQSCYHGM 
      LSQRRDQSL VFLGRSGSG KTTNFRHCI QYLVTAAGC 
      VNKILTVEK LSALWTVLE SFGNCRTVM NTNATRFTQ 
      IFSLDFDQS GVIASASIQ ILLLEKARI ARKVEGETT 
      VVKKMMLRP GNSNRYQFA NPQAAQRSA TLLGTSVEE 
      LSRVIFGLS SGGMVTPNT PRAPFRTPS PIDKGLERE 
      AVGIEAIEG MAMGLYAEV FNCIASLIN RSISASSHT 
      VSSILLVDT PGFQNPATC GRQAGASFE DLCHNYLQE 
      RLQLLYHHT NLVAPKDRY MQENIDCVY DENENLINP 
      TPLVNLMDK AAQNSVVRT SQTDLHEGD RRGLLWLLD 
      EEAIYPGST DDSFLERLF THYGDRDHQ LLLRKAPGN 
      NQFILQHLQ GTNPVLYTA KNWLKHSRE NPISRAAVT 
      VLQESCKED MSKLFVSVR GLAATSFSG SLVGIEGSQ 
      SLRRASSIR RTFTAGTAA IKRKSICLQ TKFTVDGLI 
      ETLRRTRLR FVHCLLPQH NAGFCEALA VKTSLGQQE 
      DALMNIPLL RSQIRGGQI LDAVRFYKQ GFPNFLPLS 
      EFRRRFRLL AGDGKISSP VLDERKAVE DMLLAVDLE 
      LSSYRVGLS QIFFRNGVL SQLESQRDE RLAGMVVNL 
      QAHCRGYLA RRKLTQRKL QDLAVRCIQ RNVRKFLLV 
      RDWPWWRLL VRVTPLLNV HRTEEELRM KTDELEALK 
      SKLEKLETE RTTLKHEND KLEAKLSEM TADLAEEHS 
      TSTLATERL DAETSERIR LERELSEVQ HKNKELQQS 
      SERLEMELL YARSDLNGI SEEDEDADV DGGVYKQRY 
      ERAVRELEF TKRRLQQQH EDDLEQLVG LKKQLEKKL 
      TDAYEEVEE QRQVVGQWK RKVQKLNGE MNDLRLLLE 
      EQSARNNLL EKKQRKFDS ETQQLQEEL KKERQTKDR 
      LAREKEVIM AEKYAIESS LADTRLELE LKDEKLSSL 
      QREMDEITC SGKTEEEVT QLRRQKIDC ERRLADQEE 
      ELDELAGQV QLLEQAKLR LEMSLESMR KEAKKEAQM 
      RDDELEEIR CNAQKKVKA LEAHLENEH EERTQLLRE 
      KHELERRLA AAAESERND RAGDEALLH RLKRDLKRT 
      KVLLRDTQT QLERQKAEN PGKAMIRQL KNQLEDLEC 
      ARALAVKTK QSLEADLSE TQASLDEAN RLKHEAEQK 
      VVVLAREKG DLQSQLEEN EEELAEVLK KYKTTVQQM 
      SLDQIALQE QVSLVSELE VERNHLKEQ LAELTTKLE 
      SVESMGDAS SNLLVKRTE LKVKELESK LELEQTTRG 
      RLEVQIARL KEAVEKLQS ELATARVKE QQSQDQIRK 
      LQRNLREVK EALNESLAK EAEVVQKKK ELEKRYEAL 
      EVETTTART DLKLALKRI EDLQAAIQG ELDNSTSEN 
      SDSEQDTYS SDESVSTFL ANNKSLNDF KSSSSRSST 
      GSTLGKDSS YA
    CDS sequence
    • >TC030896 RA
      atgttcaac tttatcaag aaggcgggg accggcgtg 
      acctcagat aaggacgag aaagagaag cggaaacgc 
      gacaaaaag gaccggaag gacaaacaa aaaagggaa 
      cgtgctagc atgtccgcc gaagaactg ttacgattg 
      gacgaagta cgacgatcg ttgaaaatt cgcggacgt 
      cgtaaagaa aaggaaaaa cttccttcg ggaatcaca 
      gccgattac acggccgat tttttggcg agtttagat 
      cgccaggag tcgactgtc cctcacatg aaccacctc 
      caggagggg cacacacag tccgatagc agcgaaacg 
      agcttaaac tccctaaac aacccccaa accaataaa 
      gtgttacca cccctacct ccaaaaccc ccaaaaagg 
      ggtatttta aagaccccc cgcgtcagc gtcacccaa 
      gacaacgga tacgacaat ctagtccgt aacacaatc 
      caaaacgaa ctcatcaca tatcaaaac atccccaac 
      ggcggaatc aacggcaaa aacctcggc tcggaactt 
      ttagtcata acgtcaaca tcgccaagt gctgacagt 
      ctaacagac acgacaaat tcctccttc gccacgcca 
      ccattctcc ctaagtcca gtcggggaa tcacagggt 
      tttcatcgc tggtcacgt acgtcgaat ttcgacgat 
      gttgagtta ccgttaccg gaaatcgcg ccgttaacg 
      ttaccgaaa ccgcgaacg ttaacgata caaagacag 
      aaaccgccc cggaatgat ttcgggttt tcgttgcgg 
      cgtgcaatg gttttggaa cgggttgtt gtgaacggg 
      aacggtgcc gccggtgac ggaacgtta acgatgaaa 
      gcggtcatt tttgcggaa ccgggtgct atggtgcag 
      cataataac gagacgggg ttgttaccg ggcgataag 
      ttgttggaa gtgaacggg tttccagtg gaggataag 
      acgagggag gagattatt gataagatt aaggcgtcg 
      ggggagtgt gtcacgttg aaggtccag cctgtggcg 
      gaactgagc gagttgagc agacgatcg gcccatgac 
      ggcggcgag gtcgttctg gatgattca aatatccgt 
      ggtggtaca ttacgacgt tccggaagt cgaagattc 
      aacaaatcg actacggcc aagtctgag gaacacctc 
      gaaaaggag aaacaatgg ctggacacg gaaaaagtg 
      tggttgatg caccgtggt ggtttcacg gccgccttg 
      aaaaacggc gaaaatttc accgaacct ggaaaaatc 
      caagtgtgt ctggaacac agcaaggaa attctaacc 
      gtcgacgaa gacgacatc gagaaagca aatccgccc 
      caattcgac cgtgccgaa gatttggcc gctttgcga 
      cacttgaac gagtcgagc attttacac actttgcgc 
      caacgctac gccaccaac ttgatccac acttacgcc 
      ggctgcggc accctgctg gtgatcaac cccatggcg 
      ccggtggcc atctactcg gagaaagtc gtcgctttg 
      ttcaaaggc tgcaaatcg gaagacatg ccccctcat 
      atttactcc atggcgcag tcttgctac catggcatg 
      ctctcccag cgccgtgac caatctctg gtttttctc 
      ggtcgttcc ggttcggga aaaacgaca aatttccgt 
      cactgcatt caatatttg gtgacggcg gccggttgt 
      gttaacaaa atcctgacg gtggagaaa ttaagcgcg 
      ctatggacc gttcttgaa agtttcggc aattgtcgg 
      actgttatg aacacgaat gcgacgcgt tttacgcaa 
      atttttagc ctcgatttc gatcaaagt ggtgttata 
      gcgtctgcc agtattcag atactgttg ttggagaag 
      gcgaggatt gcgaggaag gttgaggga gagacgact 
      gttgtaaaa aagatgatg ttaagacct ggtaattcg 
      aaccgttac caattcgcc aacccccaa gctgcccaa 
      cgctcggcc acccttttg ggcaccagt gtcgaggag 
      ctgtctcgt gttattttc gggctttca tctggaggc 
      atggtcact ccaaacacc ccacgagcg ccatttcgc 
      accccatcg cccattgac aagggcctt gaacgtgaa 
      gccgtgggc attgaagca atcgaaggc atggcgatg 
      ggactttac gctgaagtt ttcaactgt attgcttct 
      ctaatcaat cgttcaata tctgcatct tcacacacc 
      gtttcaagc attcttctt gtcgacacg cccggtttt 
      caaaaccca gccacttgt ggccgccaa gccggtgct 
      agttttgaa gacttatgc cacaattat ctacaagag 
      cgattgcaa cttttgtac caccatacg aatttagtg 
      gcacctaaa gaccgatac atgcaagag aatatcgat 
      tgtgtctat gacgagaat gagaattta atcaatcca 
      acaccgttg gtgaatctg atggataag gcggcgcaa 
      aatagtgtg gtgcggacg tcacaaacc gacttgcat 
      gaaggtgat cgtcgtggc cttctctgg ttgcttgat 
      gaagaagca atttatcca ggatctaca gatgattcg 
      ttccttgaa cggcttttt acacattat ggtgataga 
      gatcatcaa cttttgttg cgtaaagca ccaggaaat 
      aatcagttt attttgcaa catttgcaa ggaacgaat 
      ccggtcctg tacactgcc aaaaactgg ctcaaacat 
      agtcgagaa aatcccatt tccagggcc gccgtcaca 
      gtattacaa gagagttgc aaagaagac atgagtaag 
      ttatttgtg agcgtccga ggcctggca gccacaagt 
      tttagcggt tctttggtc ggaatcgaa ggctcgcaa 
      tccttacga agagcttca agcattcgt cgaactttt 
      acagccggt acagcggcc attaagcga aaaagcata 
      tgtttacag acgaaattt accgttgat ggtttgatt 
      gaaacttta cgacgaaca cgtttacga ttcgttcat 
      tgtcttcta cctcagcat aatgccggt ttttgtgag 
      gccttggcc gtcaaaacg agcttaggt caacaggag 
      gacgctctc atgaatatt cctttattg aggtcacaa 
      atacgcggc ggtcaaatt ttggacgct gtgcgtttt 
      tataaacag ggctttccg aattttttg ccattgagc 
      gaattcagg agacggttt cggctactg gccggtgat 
      ggtaaaatt tccagtccg gtcctggat gagaggaaa 
      gcagtggag gatatgctg ttggctgtg gatttggag 
      ctgtcaagc tacagggtc ggattaagt cagattttt 
      ttccgaaat ggggttctc tcacaattg gagtctcaa 
      agggacgaa cgtcttgcc ggtatggtg gttaacttg 
      caagctcat tgtcgcggt tatttagca agacgtaaa 
      ttaacacaa agaaaattg caagattta gccgtgaga 
      tgtatccag agaaacgtg aggaagttt cttctggtg 
      agggattgg ccatggtgg aggctactg gtgagggtg 
      acacccttg ctaaatgtg cacaggacc gaagaggaa 
      ttgaggatg aaaacggac gagttagag gcgcttaaa 
      tcaaaattg gaaaaattg gagacggaa cggacgaca 
      ttgaaacac gaaaacgac aaactggaa gccaagcta 
      agcgagatg acggccgac ttagccgaa gaacattca 
      acttcgacg ctggctaca gagcgtctc gacgccgaa 
      acgtcggaa cgcattcgt ctcgaacgc gaactgtcc 
      gaagtccag cacaagaac aaagaactg caacagtcg 
      tccgaacgt ctggaaatg gaacttttg tacgccagg 
      agtgacctg aacggcatc tccgaagag gacgaagac 
      gcggacgtg gacggcggt gtctacaaa cagcgctat 
      gaaagagcc gtccgtgaa ttggagttc accaaacgt 
      agactgcaa caacaacac gaagatgat ttagagcag 
      ttggtgggc ttgaaaaaa cagctcgag aaaaagttg 
      acagatgcg tacgaagaa gtggaagag cagagacaa 
      gttgtggga cagtggaag cgaaaagta cagaaattg 
      aacggcgaa atgaacgat ttgcgttta cttttggag 
      gaacaaagt gcgcgtaat aatttactg gagaagaag 
      caaaggaaa tttgattct gaaacgcaa cagttgcaa 
      gaagaattg aagaaggag agacagacg aaagatagg 
      cttgcgaga gagaaggaa gttatcatg gcggagaag 
      tacgccatt gagagcagt ttggcggac acgcgcctg 
      gaactcgaa ctgaaagac gaaaaactg tcctccctc 
      cagcgcgaa atggacgaa atcacctgc agcggcaaa 
      accgaagag gaagtaaca caactccgc cgccagaaa 
      atcgactgt gagcgccgt ctcgccgac caagaggaa 
      gaactcgac gaattagcc ggtcaagtc cagctcctc 
      gaacaagca aaactacgt ctggagatg tcgctggag 
      tcaatgcgt aaggaagca aaaaaagaa gcacaaatg 
      cgtgacgac gaactggaa gaaatccgc tgtaacgcc 
      cagaagaag gtcaaagct ttggaagcg cacctggag 
      aacgaacac gaggagcga acacagctt ttgcgggaa 
      aaacacgaa ctggagcga agactggcg gccgcggcc 
      gagtcggaa cgaaacgac cgcgctggc gatgaggcg 
      cttttgcat cgattgaag cgcgatttg aaacgaact 
      aaagtgctg ttgagagac acacagacg cagttggag 
      cgccagaag gcggaaaat cccggaaaa gcgatgata 
      agacagctg aaaaaccag cttgaagat ttggagtgt 
      gcgcgagct ctggctgtg aaaacaaaa cagagtctc 
      gaagccgac ttgtccgaa acacaggcg tccttggac 
      gaagccaac cgattgaaa cacgaagcc gagcaaaaa 
      gtcgtggtt ttagcgcga gaaaagggc gatcttcag 
      agccaactt gaggaaaat gaggaagaa ctggccgaa 
      gtactgaaa aaatacaaa acgacagtt cagcaaatg 
      tcactggat cagatcgcc ttgcaggag caagtgtca 
      cttgtttcg gagcttgaa gtcgaacgc aaccattta 
      aaagaacag ttggctgag cttacaaca aaactagag 
      tcggttgaa agcatgggc gatgcgtct tcaaaccta 
      ctggttaaa agaaccgaa cttaaggtc aaagaactg 
      gaatcgaag cttgaactg gaacagacc accagaggg 
      cggcttgag gtgcaaatc gctcggttg aaggaggct 
      gtggagaag ttgcaatcg gagttggcg acggcgcgt 
      gtcaaagag caacaaagt caagaccag attcggaaa 
      ttacaacgg aatttacgc gaagttaag gaggcgttg 
      aatgaaagt ttggcaaag gaagcggaa gtggtgcag 
      aagaagaag gagttggaa aagagatat gaggccttg 
      gaggtggaa acaacaacg gcaaggact gacttgaag 
      ttggcgttg aaacggatt gaggatttg caagccgcc 
      attcaaggg gaacttgat aatagcacg tcggaaaat 
      tcagacagc gagcaagac acctacagt tcggacgag 
      tctgtgagc acgtttttg gccaacaat aagtctttg 
      aacgacttt aaatcaagc agcagtcgt tcgagcact 
      ggcagtact ttaggcaag gactcgtcg tatgcctga
    mRNA sequence
    • >TC030896 RA
      ccatctctc ggcatctct ccccaacct ttcccctaa 
      cggtacatt cacaccgtg ttctatccg tctctgcgt 
      taataacgg tccgctcgt gcgcgcgac agagaaact 
      aaaccttcc aagttaaat gccagtgta aatcaagca 
      agtagtgaa aatagttga cgttcaatg tttgttgtg 
      ttcgtgtta cgtcccccg ttctgtcaa aaaactcgc 
      aaaagtggc caagataat gcgtgagtg tggtgtgtg 
      ccccactta ggttatctg tcagcaaaa acattgcaa 
      aagataagc aacggccac aatgttcaa ctttatcaa 
      gaaggcggg gaccggcgt gacctcaga taaggacga 
      gaaagagaa gcggaaacg cgacaaaaa ggaccggaa 
      ggacaaaca aaaaaggga acgtgctag catgtccgc 
      cgaagaact gttacgatt ggacgaagt acgacgatc 
      gttgaaaat tcgcggacg tcgtaaaga aaaggaaaa 
      acttccttc gggaatcac agccgatta cacggccga 
      ttttttggc gagtttaga tcgccagga gtcgactgt 
      ccctcacat gaaccacct ccaggaggg gcacacaca 
      gtccgatag cagcgaaac gagcttaaa ctccctaaa 
      caaccccca aaccaataa agtgttacc acccctacc 
      tccaaaacc cccaaaaag gggtatttt aaagacccc 
      ccgcgtcag cgtcaccca agacaacgg atacgacaa 
      tctagtccg taacacaat ccaaaacga actcatcac 
      atatcaaaa catccccaa cggcggaat caacggcaa 
      aaacctcgg ctcggaact tttagtcat aacgtcaac 
      atcgccaag tgctgacag tctaacaga cacgacaaa 
      ttcctcctt cgccacgcc accattctc cctaagtcc 
      agtcgggga atcacaggg ttttcatcg ctggtcacg 
      tacgtcgaa tttcgacga tgttgagtt accgttacc 
      ggaaatcgc gccgttaac gttaccgaa accgcgaac 
      gttaacgat acaaagaca gaaaccgcc ccggaatga 
      tttcgggtt ttcgttgcg gcgtgcaat ggttttgga 
      acgggttgt tgtgaacgg gaacggtgc cgccggtga 
      cggaacgtt aacgatgaa agcggtcat ttttgcgga 
      accgggtgc tatggtgca gcataataa cgagacggg 
      gttgttacc gggcgataa gttgttgga agtgaacgg 
      gtttccagt ggaggataa gacgaggga ggagattat 
      tgataagat taaggcgtc gggggagtg tgtcacgtt 
      gaaggtcca gcctgtggc ggaactgag cgagttgag 
      cagacgatc ggcccatga cggcggcga ggtcgttct 
      ggatgattc aaatatccg tggtggtac attacgacg 
      ttccggaag tcgaagatt caacaaatc gactacggc 
      caagtctga ggaacacct cgaaaagga gaaacaatg 
      gctggacac ggaaaaagt gtggttgat gcaccgtgg 
      tggtttcac ggccgcctt gaaaaacgg cgaaaattt 
      caccgaacc tggaaaaat ccaagtgtg tctggaaca 
      cagcaagga aattctaac cgtcgacga agacgacat 
      cgagaaagc aaatccgcc ccaattcga ccgtgccga 
      agatttggc cgctttgcg acacttgaa cgagtcgag 
      cattttaca cactttgcg ccaacgcta cgccaccaa 
      cttgatcca cacttacgc cggctgcgg caccctgct 
      ggtgatcaa ccccatggc gccggtggc catctactc 
      ggagaaagt cgtcgcttt gttcaaagg ctgcaaatc 
      ggaagacat gccccctca tatttactc catggcgca 
      gtcttgcta ccatggcat gctctccca gcgccgtga 
      ccaatctct ggtttttct cggtcgttc cggttcggg 
      aaaaacgac aaatttccg tcactgcat tcaatattt 
      ggtgacggc ggccggttg tgttaacaa aatcctgac 
      ggtggagaa attaagcgc gctatggac cgttcttga 
      aagtttcgg caattgtcg gactgttat gaacacgaa 
      tgcgacgcg ttttacgca aatttttag cctcgattt 
      cgatcaaag tggtgttat agcgtctgc cagtattca 
      gatactgtt gttggagaa ggcgaggat tgcgaggaa 
      ggttgaggg agagacgac tgttgtaaa aaagatgat 
      gttaagacc tggtaattc gaaccgtta ccaattcgc 
      caaccccca agctgccca acgctcggc caccctttt 
      gggcaccag tgtcgagga gctgtctcg tgttatttt 
      cgggctttc atctggagg catggtcac tccaaacac 
      cccacgagc gccatttcg caccccatc gcccattga 
      caagggcct tgaacgtga agccgtggg cattgaagc 
      aatcgaagg catggcgat gggacttta cgctgaagt 
      tttcaactg tattgcttc tctaatcaa tcgttcaat 
      atctgcatc ttcacacac cgtttcaag cattcttct 
      tgtcgacac gcccggttt tcaaaaccc agccacttg 
      tggccgcca agccggtgc tagttttga agacttatg 
      ccacaatta tctacaaga gcgattgca acttttgta 
      ccaccatac gaatttagt ggcacctaa agaccgata 
      catgcaaga gaatatcga ttgtgtcta tgacgagaa 
      tgagaattt aatcaatcc aacaccgtt ggtgaatct 
      gatggataa ggcggcgca aaatagtgt ggtgcggac 
      gtcacaaac cgacttgca tgaaggtga tcgtcgtgg 
      ccttctctg gttgcttga tgaagaagc aatttatcc 
      aggatctac agatgattc gttccttga acggctttt 
      tacacatta tggtgatag agatcatca acttttgtt 
      gcgtaaagc accaggaaa taatcagtt tattttgca 
      acatttgca aggaacgaa tccggtcct gtacactgc 
      caaaaactg gctcaaaca tagtcgaga aaatcccat 
      ttccagggc cgccgtcac agtattaca agagagttg 
      caaagaaga catgagtaa gttatttgt gagcgtccg 
      aggcctggc agccacaag ttttagcgg ttctttggt 
      cggaatcga aggctcgca atccttacg aagagcttc 
      aagcattcg tcgaacttt tacagccgg tacagcggc 
      cattaagcg aaaaagcat atgtttaca gacgaaatt 
      taccgttga tggtttgat tgaaacttt acgacgaac 
      acgtttacg attcgttca ttgtcttct acctcagca 
      taatgccgg tttttgtga ggccttggc cgtcaaaac 
      gagcttagg tcaacagga ggacgctct catgaatat 
      tcctttatt gaggtcaca aatacgcgg cggtcaaat 
      tttggacgc tgtgcgttt ttataaaca gggctttcc 
      gaatttttt gccattgag cgaattcag gagacggtt 
      tcggctact ggccggtga tggtaaaat ttccagtcc 
      ggtcctgga tgagaggaa agcagtgga ggatatgct 
      gttggctgt ggatttgga gctgtcaag ctacagggt 
      cggattaag tcagatttt tttccgaaa tggggttct 
      ctcacaatt ggagtctca aagggacga acgtcttgc 
      cggtatggt ggttaactt gcaagctca ttgtcgcgg 
      ttatttagc aagacgtaa attaacaca aagaaaatt 
      gcaagattt agccgtgag atgtatcca gagaaacgt 
      gaggaagtt tcttctggt gagggattg gccatggtg 
      gaggctact ggtgagggt gacaccctt gctaaatgt 
      gcacaggac cgaagagga attgaggat gaaaacgga 
      cgagttaga ggcgcttaa atcaaaatt ggaaaaatt 
      ggagacgga acggacgac attgaaaca cgaaaacga 
      caaactgga agccaagct aagcgagat gacggccga 
      cttagccga agaacattc aacttcgac gctggctac 
      agagcgtct cgacgccga aacgtcgga acgcattcg 
      tctcgaacg cgaactgtc cgaagtcca gcacaagaa 
      caaagaact gcaacagtc gtccgaacg tctggaaat 
      ggaactttt gtacgccag gagtgacct gaacggcat 
      ctccgaaga ggacgaaga cgcggacgt ggacggcgg 
      tgtctacaa acagcgcta tgaaagagc cgtccgtga 
      attggagtt caccaaacg tagactgca acaacaaca 
      cgaagatga tttagagca gttggtggg cttgaaaaa 
      acagctcga gaaaaagtt gacagatgc gtacgaaga 
      agtggaaga gcagagaca agttgtggg acagtggaa 
      gcgaaaagt acagaaatt gaacggcga aatgaacga 
      tttgcgttt acttttgga ggaacaaag tgcgcgtaa 
      taatttact ggagaagaa gcaaaggaa atttgattc 
      tgaaacgca acagttgca agaagaatt gaagaagga 
      gagacagac gaaagatag gcttgcgag agagaagga 
      agttatcat ggcggagaa gtacgccat tgagagcag 
      tttggcgga cacgcgcct ggaactcga actgaaaga 
      cgaaaaact gtcctccct ccagcgcga aatggacga 
      aatcacctg cagcggcaa aaccgaaga ggaagtaac 
      acaactccg ccgccagaa aatcgactg tgagcgccg 
      tctcgccga ccaagagga agaactcga cgaattagc 
      cggtcaagt ccagctcct cgaacaagc aaaactacg 
      tctggagat gtcgctgga gtcaatgcg taaggaagc 
      aaaaaaaga agcacaaat gcgtgacga cgaactgga 
      agaaatccg ctgtaacgc ccagaagaa ggtcaaagc 
      tttggaagc gcacctgga gaacgaaca cgaggagcg 
      aacacagct tttgcggga aaaacacga actggagcg 
      aagactggc ggccgcggc cgagtcgga acgaaacga 
      ccgcgctgg cgatgaggc gcttttgca tcgattgaa 
      gcgcgattt gaaacgaac taaagtgct gttgagaga 
      cacacagac gcagttgga gcgccagaa ggcggaaaa 
      tcccggaaa agcgatgat aagacagct gaaaaacca 
      gcttgaaga tttggagtg tgcgcgagc tctggctgt 
      gaaaacaaa acagagtct cgaagccga cttgtccga 
      aacacaggc gtccttgga cgaagccaa ccgattgaa 
      acacgaagc cgagcaaaa agtcgtggt tttagcgcg 
      agaaaaggg cgatcttca gagccaact tgaggaaaa 
      tgaggaaga actggccga agtactgaa aaaatacaa 
      aacgacagt tcagcaaat gtcactgga tcagatcgc 
      cttgcagga gcaagtgtc acttgtttc ggagcttga 
      agtcgaacg caaccattt aaaagaaca gttggctga 
      gcttacaac aaaactaga gtcggttga aagcatggg 
      cgatgcgtc ttcaaacct actggttaa aagaaccga 
      acttaaggt caaagaact ggaatcgaa gcttgaact 
      ggaacagac caccagagg gcggcttga ggtgcaaat 
      cgctcggtt gaaggaggc tgtggagaa gttgcaatc 
      ggagttggc gacggcgcg tgtcaaaga gcaacaaag 
      tcaagacca gattcggaa attacaacg gaatttacg 
      cgaagttaa ggaggcgtt gaatgaaag tttggcaaa 
      ggaagcgga agtggtgca gaagaagaa ggagttgga 
      aaagagata tgaggcctt ggaggtgga aacaacaac 
      ggcaaggac tgacttgaa gttggcgtt gaaacggat 
      tgaggattt gcaagccgc cattcaagg ggaacttga 
      taatagcac gtcggaaaa ttcagacag cgagcaaga 
      cacctacag ttcggacga gtctgtgag cacgttttt 
      ggccaacaa taagtcttt gaacgactt taaatcaag 
      cagcagtcg ttcgagcac tggcagtac tttaggcaa 
      ggactcgtc gtatgcctg agtggcggc gaacgtctg 
      tcgaacgat ttcgatgac gttctgcag acaagagga 
      tgaagtcgc ttctgatta cgtttgatc agaacttgt 
      ttatttttt attattgtg atataagac tgagatgat 
      ttcgagttt ttatacaac tgttgtaat cgtacttaa 
      cgctctcaa ttactagga aaaaatcaa aagaatgaa 
      atgtgacgc aatcaaact ccttagaag gggccaggt 
      tatcattta tctactatc agttgtaat tttttgtgt 
      atagtgggc gattttttg gaggaaaat cgttgataa 
      tatattctt tatagacat gtactgtat atacgcttc 
      ccagacaca cacagacaa tgttcatag taatcggtc 
      gatgtatta tagcagttg gaacgtaat aagtgttag 
      gttttaaat tttttaagt ctcttctgt acctagaca 
      ttttaccat attctgtat tataatttc aatgttgtg 
      gaaatatat tgtttacaa atat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY