Status
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Gene information
General information
TC033339
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC015266,TC015265
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 9636275..9640528
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033339
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02443

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
    head partially remaining - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva segment decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033339 RA
      MKLILFLIV LIKSDLVVL GTTEPQVVL PTLGVLQGG 
      FKTSLKGRT FYSFEGVPY ARPPVGKYR FREPVPPKP 
      WHGIWEAKT IHKCMQYYQ YTPPGGDFV IGDEDCLYL 
      NIYTPKLDT KANLDVIVY IHGGAFMFN WGGIQGPEY 
      LLDKDVVYV NLNYRLGPL GFLSTENDL IPGNNGLKD 
      QILALEWVQ NHIVHFGGN PNSVTLVGM SAGGASVHF 
      HYLSPKSRG LFHRGMSQS GTMLNAWVL MEQPMEKTQ 
      KLAILLGCP SDGKPMVEC LKRRPGRQI VEMVKYYQP 
      WLYNPFSPF GVVVDSWSK DPVLPDHPY SLIKNKQLV 
      DLPWITSYT SSEGLYPAS DFYIQEQYL NEIDTKWNQ 
      LLPFILDYN YTLDPKKHD EISQKIRRY YLGEKTVNR 
      DNFLDFVRI LTDRLFVYD IQKTVFLQN QVTKSPIFL 
      YYFTYRGAH SKSEGRSGT SKDLGAAHG DDTSYIFKT 
      KVDTTSTRQ DREMVELFV EMLASFAKS GVPRVPIAW 
      PQVPQTISQ LTYLKIASP DNLTLETSV LGSTQFWDS 
      LPIEENEKL LHGVKDEFS VLTKMRLVL LAIFLTACQ 
      AELIVELPN LGKVEGRLT TMNGLKVHR FAGIPYAQP 
      PVGENRFEA PKPVEPWDG VWKPDTTYR CMQFTRLPH 
      MDEDHVIGE EDCLYLTVY TAHLNASFD VVVYIHGGD 
      FATGYASVY EPDYFIDKN IVFVNLNYR LGPLGFLST 
      EDDVVPGNN GLKDQILAL KFIKKYIQH FGGTPDSIT 
      LFGDGTSVN FHYLSPQSR GLFHRGWSM SGTMLVPWA 
      LMEQPLAQT KKLATIVGC GTDETMIKC LKSRPARQI 
      ALTVPRYQA WWYLSFAQF GPVIDSWAT QPVLPTHPY 
      QIIKNQQVY DVPWIASFT KSEGLYHLD EVYNNQLLT 
      KLDDNWNKY IPAILYYNY TVDRDLQDE VSQKIRKYY 
      LGEKKLSKK TFRELVAMF SDRIFINDI VKTAKLMAK 
      SMKSPTYAL KFEYRSMNS WFEHETESG RNFGAAHAD 
      DVPYIFNMG LFDTSRNHR DRAMIKIFV DLLTSFSDS 
      SIPKVKKIE WPQVPKNAT DDLTYLRIG APSDVTVET 
      SIRSTASFW DSLPIKENE KLP
    CDS sequence
    • >TC033339 RA
      atgaagtta attttgttc ctaatagtg ctaattaaa 
      agcgatttg gtggtactt ggtaccact gagccacaa 
      gtggtacta ccaactttg ggagtactc caaggaggt 
      tttaaaaca agtctcaaa ggtcgtacg ttttattcg 
      tttgaagga gtaccttat gcaaggcca ccggtcgga 
      aagtaccgt ttccgggaa ccagtacca ccgaaacct 
      tggcatggc atatgggag gccaaaacc atccacaag 
      tgtatgcag tactaccaa tatacaccc cctgggggt 
      gatttcgta attggcgat gaggattgt ctctacttg 
      aacatttac accccaaag ttggacact aaggccaat 
      ttggacgtc atagtgtac atccatggg ggcgccttt 
      atgttcaac tggggcggc atccaaggg ccggagtac 
      ctcctagac aaggatgta gtttacgtg aatttgaac 
      taccgttta ggacctttg ggttttttg agtactgaa 
      aatgaccta attccggga aacaacggt ttgaaagac 
      cagattttg gccttggaa tgggtccaa aaccatata 
      gtccatttc gggggaaac ccaaattct gtgactttg 
      gttggtatg tcagcaggg ggtgctagt gttcatttt 
      cactactta tcgcccaag tcgcgtggt ttgttccat 
      cgaggcatg tcacaaagt ggtaccatg ctcaatgcc 
      tgggtacta atggaacaa ccaatggaa aaaacccag 
      aaattggcc attcttttg gggtgtccc agtgatggt 
      aagccaatg gttgagtgt ttgaaacga aggccgggc 
      cgacaaatt gtggaaatg gtcaagtac taccaacca 
      tggttgtat aacccgttt agtccattc ggtgttgtg 
      gtggattcg tggtcgaaa gacccagtc ttgcccgac 
      cacccttac agtttgata aaaaacaaa cagttggtt 
      gatttgccc tggattacg tcttacacg agttctgaa 
      gggttgtac ccagcatcc gacttctat atccaggag 
      cagtacctt aacgaaatt gacaccaag tggaatcaa 
      cttttacca ttcattctg gactacaat tatacactt 
      gaccccaaa aaacacgat gagattagc caaaaaatt 
      cggaggtac taccttggg gagaaaaca gtcaaccgg 
      gacaatttc ctcgacttt gtccgaatt ttaaccgat 
      cgtttgttc gtctacgac atccagaaa acggtattt 
      ttgcaaaac caagtcaca aaatcgccg atttttttg 
      tactacttt acgtacaga ggtgctcat agtaagtct 
      gaaggacgt tcgggtact agtaaagac ttaggcgcc 
      gcccacggc gatgatact agctatatt ttcaaaaca 
      aaagttgat actactagt actaggcaa gaccgggaa 
      atggtcgag ttgtttgtt gaaatgttg gcctcattt 
      gccaaatca ggggtacca cgagtacca atcgcctgg 
      ccacaagta cctcaaact attagtcaa ctgacttat 
      ctaaaaatt gcttccccg gataatctc acgttggaa 
      acgtcggta cttggtagt acccagttt tgggattcg 
      ttgccaatt gaagaaaac gagaagctg ctccatggt 
      gtcaaagat gaattctca gtcttaacc aaaatgcgc 
      ctggtacta cttgcgata ttcctgact gcttgtcag 
      gcggaacta atcgtcgaa ttgcccaat ttgggtaaa 
      gttgaaggt cggctaacc accatgaat gggctaaaa 
      gtccaccgg tttgcggga ataccatat gcccaacca 
      ccggttgga gaaaaccga tttgaggca cccaaaccg 
      gtggaacca tgggatggt gtttggaaa cctgatacc 
      acttacaga tgtatgcag tttaccaga ctcccacat 
      atggacgaa gaccatgtg attggtgag gaggattgt 
      ctttatttg accgtttac acggcccac ttgaatgct 
      agttttgat gtggttgtg tacattcat ggtggtgat 
      ttcgcgact ggttatgcc agtgtttac gaacctgac 
      tattttata gacaaaaat atagttttc gtaaacttg 
      aattataga ttagggccg ttaggtttt ttgagtact 
      gaggatgac gtagtacct ggcaataat gggctcaaa 
      gaccaaatc ctggcctta aaattcatc aaaaaatat 
      atccaacat ttcggtggt actcctgac tcgatcaca 
      cttttcgga gatggtacc agtgttaac ttccattac 
      ttatcgcca caatcccgt ggcttgttc caccgtggt 
      tggtccatg agtggtacc atgttagta ccatgggcc 
      ctaatggag caacctttg gcccaaacg aaaaaactc 
      gccactatc gtgggttgt ggtaccgat gagacaatg 
      atcaagtgt ttgaaaagt agaccagcc aggcaaatt 
      gccttgaca gtaccacgg taccaagct tggtggtac 
      ctgtcattt gcccaattc gggcccgtg attgactcc 
      tgggccact caaccggta ctaccaacc catccctac 
      caaatcatc aaaaatcaa caagtctat gacgtccct 
      tggatcgcc tcttttaca aaatcggaa gggttgtac 
      catttagac gaagtctac aataatcaa cttttgaca 
      aagcttgac gacaactgg aataagtac attccagct 
      attttgtac tataattac accgtggat cgggatttg 
      caagatgag gtgagtcag aagattagg aagtactac 
      cttggagag aagaaattg tcaaagaag acgtttcgg 
      gagttggtg gcgatgttt tcagaccgg atttttatc 
      aacgatatc gtcaaaacc gccaaactg atggcaaag 
      tcaatgaaa tcccctacc tatgccttg aaatttgaa 
      tacaggagt atgaatagt tggttcgag catgaaaca 
      gagagtggg aggaatttt ggagcagct catgctgat 
      gacgtccct tatattttc aacatggga ctttttgat 
      acaagcaga aaccaccga gacagagct atgattaaa 
      atttttgtc gatttgtta acatcgttt agtgactcg 
      agcattcca aaagtaaaa aaaatagag tggccacaa 
      gtaccaaaa aatgccaca gacgacttg acctatcta 
      agaattgga gccccgtct gacgtaact gtggaaacg 
      tcaattcga agcactgcc tctttttgg gattcgcta 
      ccaattaaa gaaaatgaa aaattaccc taa
    mRNA sequence
    • >TC033339 RA
      actatcaaa acaaaaaac atcggaatt atatttagt 
      tgacctcaa acattattc aagcgtctt gaaaacatg 
      aagttaatt ttgttccta atagtgcta attaaaagc 
      gatttggtg gtacttggt accactgag ccacaagtg 
      gtactacca actttggga gtactccaa ggaggtttt 
      aaaacaagt ctcaaaggt cgtacgttt tattcgttt 
      gaaggagta ccttatgca aggccaccg gtcggaaag 
      taccgtttc cgggaacca gtaccaccg aaaccttgg 
      catggcata tgggaggcc aaaaccatc cacaagtgt 
      atgcagtac taccaatat acaccccct gggggtgat 
      ttcgtaatt ggcgatgag gattgtctc tacttgaac 
      atttacacc ccaaagttg gacactaag gccaatttg 
      gacgtcata gtgtacatc catgggggc gcctttatg 
      ttcaactgg ggcggcatc caagggccg gagtacctc 
      ctagacaag gatgtagtt tacgtgaat ttgaactac 
      cgtttagga cctttgggt tttttgagt actgaaaat 
      gacctaatt ccgggaaac aacggtttg aaagaccag 
      attttggcc ttggaatgg gtccaaaac catatagtc 
      catttcggg ggaaaccca aattctgtg actttggtt 
      ggtatgtca gcagggggt gctagtgtt cattttcac 
      tacttatcg cccaagtcg cgtggtttg ttccatcga 
      ggcatgtca caaagtggt accatgctc aatgcctgg 
      gtactaatg gaacaacca atggaaaaa acccagaaa 
      ttggccatt cttttgggg tgtcccagt gatggtaag 
      ccaatggtt gagtgtttg aaacgaagg ccgggccga 
      caaattgtg gaaatggtc aagtactac caaccatgg 
      ttgtataac ccgtttagt ccattcggt gttgtggtg 
      gattcgtgg tcgaaagac ccagtcttg cccgaccac 
      ccttacagt ttgataaaa aacaaacag ttggttgat 
      ttgccctgg attacgtct tacacgagt tctgaaggg 
      ttgtaccca gcatccgac ttctatatc caggagcag 
      taccttaac gaaattgac accaagtgg aatcaactt 
      ttaccattc attctggac tacaattat acacttgac 
      cccaaaaaa cacgatgag attagccaa aaaattcgg 
      aggtactac cttggggag aaaacagtc aaccgggac 
      aatttcctc gactttgtc cgaatttta accgatcgt 
      ttgttcgtc tacgacatc cagaaaacg gtatttttg 
      caaaaccaa gtcacaaaa tcgccgatt tttttgtac 
      tactttacg tacagaggt gctcatagt aagtctgaa 
      ggacgttcg ggtactagt aaagactta ggcgccgcc 
      cacggcgat gatactagc tatattttc aaaacaaaa 
      gttgatact actagtact aggcaagac cgggaaatg 
      gtcgagttg tttgttgaa atgttggcc tcatttgcc 
      aaatcaggg gtaccacga gtaccaatc gcctggcca 
      caagtacct caaactatt agtcaactg acttatcta 
      aaaattgct tccccggat aatctcacg ttggaaacg 
      tcggtactt ggtagtacc cagttttgg gattcgttg 
      ccaattgaa gaaaacgag aagctgctc catggtgtc 
      aaagatgaa ttctcagtc ttaaccaaa atgcgcctg 
      gtactactt gcgatattc ctgactgct tgtcaggcg 
      gaactaatc gtcgaattg cccaatttg ggtaaagtt 
      gaaggtcgg ctaaccacc atgaatggg ctaaaagtc 
      caccggttt gcgggaata ccatatgcc caaccaccg 
      gttggagaa aaccgattt gaggcaccc aaaccggtg 
      gaaccatgg gatggtgtt tggaaacct gataccact 
      tacagatgt atgcagttt accagactc ccacatatg 
      gacgaagac catgtgatt ggtgaggag gattgtctt 
      tatttgacc gtttacacg gcccacttg aatgctagt 
      tttgatgtg gttgtgtac attcatggt ggtgatttc 
      gcgactggt tatgccagt gtttacgaa cctgactat 
      tttatagac aaaaatata gttttcgta aacttgaat 
      tatagatta gggccgtta ggttttttg agtactgag 
      gatgacgta gtacctggc aataatggg ctcaaagac 
      caaatcctg gccttaaaa ttcatcaaa aaatatatc 
      caacatttc ggtggtact cctgactcg atcacactt 
      ttcggagat ggtaccagt gttaacttc cattactta 
      tcgccacaa tcccgtggc ttgttccac cgtggttgg 
      tccatgagt ggtaccatg ttagtacca tgggcccta 
      atggagcaa cctttggcc caaacgaaa aaactcgcc 
      actatcgtg ggttgtggt accgatgag acaatgatc 
      aagtgtttg aaaagtaga ccagccagg caaattgcc 
      ttgacagta ccacggtac caagcttgg tggtacctg 
      tcatttgcc caattcggg cccgtgatt gactcctgg 
      gccactcaa ccggtacta ccaacccat ccctaccaa 
      atcatcaaa aatcaacaa gtctatgac gtcccttgg 
      atcgcctct tttacaaaa tcggaaggg ttgtaccat 
      ttagacgaa gtctacaat aatcaactt ttgacaaag 
      cttgacgac aactggaat aagtacatt ccagctatt 
      ttgtactat aattacacc gtggatcgg gatttgcaa 
      gatgaggtg agtcagaag attaggaag tactacctt 
      ggagagaag aaattgtca aagaagacg tttcgggag 
      ttggtggcg atgttttca gaccggatt tttatcaac 
      gatatcgtc aaaaccgcc aaactgatg gcaaagtca 
      atgaaatcc cctacctat gccttgaaa tttgaatac 
      aggagtatg aatagttgg ttcgagcat gaaacagag 
      agtgggagg aattttgga gcagctcat gctgatgac 
      gtcccttat attttcaac atgggactt tttgataca 
      agcagaaac caccgagac agagctatg attaaaatt 
      tttgtcgat ttgttaaca tcgtttagt gactcgagc 
      attccaaaa gtaaaaaaa atagagtgg ccacaagta 
      ccaaaaaat gccacagac gacttgacc tatctaaga 
      attggagcc ccgtctgac gtaactgtg gaaacgtca 
      attcgaagc actgcctct ttttgggat tcgctacca 
      attaaagaa aatgaaaaa ttaccctaa ttattaaat 
      aaatcgtac aaaaatta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY