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Gene information
General information
TC015257 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 9549575..9551132
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015257
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02442

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    potentially confused/crosscontaminated with : iB # (comma separated) 27.N2 adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    potentially confused/crosscontaminated with : adults of..., iB # (comma separated) 27.N2
    Pupal morphology
    potentially confused/crosscontaminated with : iB # (comma separated) 27.N2
    Adult morphology
    potentially confused/crosscontaminated with : adults of..., iB # (comma separated) 27.N2
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    potentially confused/crosscontaminated with : adults of..., iB # (comma separated) 27.N2
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015257 RA
      MPPKKHRHL TAEEKRELR KQKKLEKKQ RLAEKKKQL 
      KRDHLAREV NYGQLTIKK YEKNWREML IKVALPRMR 
      QELEFAWHN FERVVDCKD FTISLLMDE LRDSEEQYM 
      LNFRAHSEN MEKLMWLFH DRLEELQAD FESECKLMQ 
      ERCENEFQD ARELYNENF DYLKTMLHQ LELERREHN 
      RLKKGEYFS RLDEVEVKN QLLLQRLRA TLERKYQNM 
      FEDMKRFIK NYNKQIRER KKEHNLLKD QDDSIQELI 
      AQQFNNIKK CYDRIRQLR QKYTDMKQI DGSRLSDLQ 
      AERNYFSNS FMTLKTKLE NDKKDDFGK LALLSENAA 
      KAFDCLEHL NKRGEMILT LAAVCRKLE TQKEKILPF 
      PRTTTNLPE IADIIDRDD AEEIEGVRP EMELFWQRL 
      GQAFGVHYT LMREKNCLS DENQLLADT HNEYCERLM 
      YPPIHSYMG RVTIPKTDT KLEMAKYDK FRGHTHKPF 
      VHVTHRQQK
    CDS sequence
    • >TC015257 RA
      atgccgcca aaaaagcac cgtcatctc accgccgaa 
      gagaagagg gagctgaga aaacagaag aagcttgag 
      aagaaacag cgcttggct gagaagaag aagcagctc 
      aagagggac catctggcc cgtgaagtg aattatgga 
      caactcact atcaagaaa tacgagaaa aattggagg 
      gaaatgctc attaaagtc gctcttccg cgaatgcgg 
      caagagttg gagtttgcg tggcataat tttgagcga 
      gttgttgac tgcaaggac tttaccatc tccttgctg 
      atggacgag ttgagggac tcggaggag cagtatatg 
      ctcaatttt cgggcgcat tccgaaaat atggaaaag 
      ctgatgtgg ctgtttcac gacaggttg gaggagctt 
      caagccgat tttgaatcg gaatgcaaa ttgatgcaa 
      gaacggtgc gaaaacgag tttcaagac gctagagag 
      ctgtacaac gagaatttt gattatctg aaaacgatg 
      cttcaccag ctcgagctg gaacgccgg gaacacaac 
      cggctgaaa aaaggggaa tatttctcc agactggac 
      gaagttgaa gtgaaaaac cagctactt ttgcaaagg 
      ttgcgagcc acacttgag cgcaaatac cagaacatg 
      ttcgaagac atgaaacgc tttatcaaa aactacaac 
      aagcagatt agagagcgc aaaaaggag cacaatttg 
      ctgaaggat caagacgat tcaatccag gaactaatc 
      gcccaacaa ttcaacaac attaagaaa tgttacgat 
      cggattcgc caactccgc caaaagtac accgacatg 
      aagcagatt gatggtagc agattgtcc gatttgcag 
      gctgaaaga aactatttt tctaacagt tttatgacg 
      cttaaaacg aaattggaa aatgacaag aaggacgat 
      ttcggcaaa cttgccctt ttgagcgaa aacgcagcc 
      aaagccttc gattgttta gagcatttg aataagagg 
      ggagaaatg attcttact ttggctgca gtttgccgg 
      aaacttgag acacagaag gagaaaatt ctgccgttt 
      ccgcgcacc acaacaaat cttccggaa attgcagat 
      attattgat agggatgat gcggaggag attgaaggg 
      gttaggcct gagatggag ctgttttgg caaaggctg 
      gggcaggct tttggtgtt cattatact ttgatgagg 
      gagaaaaac tgtctaagt gacgaaaat cagttgttg 
      gcagatacg cataatgaa tattgtgag agattgatg 
      tatccgccc attcatagt tacatggga agggtgaca 
      attcccaaa actgatact aagttggaa atggcaaag 
      tatgataag tttagaggg cacacgcat aagccgttt 
      gtgcatgtc acacaccga caacagaaa taa
    mRNA sequence
    • >TC015257 RA
      acacatagc aggctgtga ttttttgac aaaatgccg 
      ccaaaaaag caccgtcat ctcaccgcc gaagagaag 
      agggagctg agaaaacag aagaagctt gagaagaaa 
      cagcgcttg gctgagaag aagaagcag ctcaagagg 
      gaccatctg gcccgtgaa gtgaattat ggacaactc 
      actatcaag aaatacgag aaaaattgg agggaaatg 
      ctcattaaa gtcgctctt ccgcgaatg cggcaagag 
      ttggagttt gcgtggcat aattttgag cgagttgtt 
      gactgcaag gactttacc atctccttg ctgatggac 
      gagttgagg gactcggag gagcagtat atgctcaat 
      tttcgggcg cattccgaa aatatggaa aagctgatg 
      tggctgttt cacgacagg ttggaggag cttcaagcc 
      gattttgaa tcggaatgc aaattgatg caagaacgg 
      tgcgaaaac gagtttcaa gacgctaga gagctgtac 
      aacgagaat tttgattat ctgaaaacg atgcttcac 
      cagctcgag ctggaacgc cgggaacac aaccggctg 
      aaaaaaggg gaatatttc tccagactg gacgaagtt 
      gaagtgaaa aaccagcta cttttgcaa aggttgcga 
      gccacactt gagcgcaaa taccagaac atgttcgaa 
      gacatgaaa cgctttatc aaaaactac aacaagcag 
      attagagag cgcaaaaag gagcacaat ttgctgaag 
      gatcaagac gattcaatc caggaacta atcgcccaa 
      caattcaac aacattaag aaatgttac gatcggatt 
      cgccaactc cgccaaaag tacaccgac atgaagcag 
      attgatggt agcagattg tccgatttg caggctgaa 
      agaaactat ttttctaac agttttatg acgcttaaa 
      acgaaattg gaaaatgac aagaaggac gatttcggc 
      aaacttgcc cttttgagc gaaaacgca gccaaagcc 
      ttcgattgt ttagagcat ttgaataag aggggagaa 
      atgattctt actttggct gcagtttgc cggaaactt 
      gagacacag aaggagaaa attctgccg tttccgcgc 
      accacaaca aatcttccg gaaattgca gatattatt 
      gatagggat gatgcggag gagattgaa ggggttagg 
      cctgagatg gagctgttt tggcaaagg ctggggcag 
      gcttttggt gttcattat actttgatg agggagaaa 
      aactgtcta agtgacgaa aatcagttg ttggcagat 
      acgcataat gaatattgt gagagattg atgtatccg 
      cccattcat agttacatg ggaagggtg acaattccc 
      aaaactgat actaagttg gaaatggca aagtatgat 
      aagtttaga gggcacacg cataagccg tttgtgcat 
      gtcacacac cgacaacag aaataaaat ttgcactgt 
      gtttatttt a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY