Status
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Gene information
General information
TC015256 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 9528285..9532759
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015256
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02441

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    head size decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    antenna antennal flagellum decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015256 RA
      MKQLCEGLS YLHTQRILH RDIKPQNLL VDKEGHIKI 
      ADFGLSRCF SIPTKPYTH EVVTMWYRA PELLLGSKL 
      YTNGIDVWS LGCVMVEML LKRALFPGD SEIDQMFKI 
      FKTLGTPNE EMWPGVSSL PAYEVWFPQ WKPCELNTQ 
      IKFNDSEEE EFLKLLLVY DPFVRKSAK DLLSLPYIK 
      RAKLTTPDM KPFPDEEED SELLKCEVL STSQSVFGV 
      LPSMECFIS SKNVVKLCR ICLTSSNNL LSLDSPVTG 
      NNNDFANSI HHLIETVTL EKVIDDTLL PSKICPICL 
      SYLKIAFEF QTQFKKSQE ILHKTLKIE VPPRNNNTS 
      VVQQPVSTP TVELICGES KFDLNDIII IEENQADRT 
      SFQGFLSNL GTEISASFL GESHKNVQI PEPVENDVV 
      ILPIKHEQK IADVEQDVL DISRLSVTK VAEESKVII 
      KNNNKVVKT LYECQTCKK ILPNRQQLN FHFSTHAKR 
      TCYVCDKCG HIAKTRSAF RHHLAKHSG VVFKCEYCG 
      KCFDGKSHF KIHVEAHKN NRPFLCNVC GKTFNYNTS 
      LIYHMRIHT GEKKYVCSF CGLRYRMGT TLKRHVRTH 
      TKERPFGCQ YCVKRFSSK NELVNHEMV HLGIRPYQC 
      KYCERCFTK SVNLDTHYL THSGPHECQ YCVKTFLEI 
      RFLKMHLKR VHKELEVMT DEVEN
    CDS sequence
    • >TC015256 RA
      atgaaacaa ttatgcgaa ggcctttcc tatttacac 
      acccagcgc atccttcac cgtgatatc aagccccaa 
      aacttgcta gtcgataaa gaaggccac atcaaaatc 
      gccgacttt ggcctctct cgctgtttt tccatacca 
      acaaaaccg tacacccac gaagtggtg acaatgtgg 
      tacagagcg cctgaactg ctcctaggt tccaagctg 
      tacaccaat gggatcgac gtgtggagt cttgggtgt 
      gttatggta gaaatgttg ttaaaacgt gcccttttc 
      cccggtgac tcagaaatc gaccaaatg ttcaaaatt 
      ttcaaaact ctgggaacg ccaaatgag gaaatgtgg 
      ccgggggtg tcgtcactg ccggcctat gaggtctgg 
      ttcccgcag tggaagcca tgcgagctt aacactcaa 
      attaaattc aacgattcg gaggaagaa gagtttctt 
      aaattgttg ttggtttat gatccgttt gtgcgaaag 
      tcggccaaa gatttgcta agtctgccc tatatcaag 
      agagccaag ctcacgacg cctgatatg aagccgttt 
      cctgacgaa gaggaggac tctgaactt ttaaaatgt 
      gaggttcta tcaacaagt caaagtgtt tttggtgtt 
      ttgccttca atggaatgt tttattagc tccaaaaac 
      gtggtcaag ttgtgccgt atttgcctc acatcttca 
      aacaaccta ctatcattg gactctcca gtcactgga 
      aacaacaac gatttcgca aactccata caccacctc 
      attgaaacc gtaactttg gaaaaagtc atagatgac 
      acactacta ccatcgaaa atttgccca atatgtctc 
      agctacttg aaaatagcg ttcgaattt caaacacag 
      ttcaaaaag tcgcaggaa attttgcac aaaacgcta 
      aaaattgag gtaccacct agaaataac aacacgtcg 
      gtggtgcaa caacctgtg tccacaccc actgttgaa 
      ttaatctgt ggtgagagt aaattcgat ttgaacgat 
      attatcata attgaggag aatcaagcc gaccggacg 
      tctttccaa ggctttttg agtaactta ggtacggaa 
      atatcggcc tcattcctt ggcgaaagc cacaaaaat 
      gttcaaatt cccgaacca gttgaaaac gacgtagtt 
      atattacca ataaaacac gaacaaaaa atagcagac 
      gtggaacaa gatgtgctt gatatcagt cgcttgagc 
      gtaacaaaa gtggccgaa gagagcaaa gttataata 
      aaaaacaat aacaaagtc gtaaaaaca ctgtacgag 
      tgtcagacg tgtaaaaaa attcttcca aatcggcaa 
      caattaaac tttcacttt tcaacccat gcaaagcga 
      acgtgttac gtttgtgac aaatgtgga cacattgcc 
      aagacacgg tctgccttc cgccaccat ttagccaaa 
      cacagtggg gttgtgttc aagtgcgag tattgcggg 
      aagtgcttt gacggtaaa tcgcacttt aaaatccac 
      gtggaagcg cacaaaaac aaccggccg tttttgtgc 
      aacgtctgt gggaaaacc ttcaattac aacacgtcg 
      ctcatctac cacatgagg atccacaca ggggagaag 
      aagtacgtt tgcagcttc tgtggcttg agataccgt 
      atggggacg acgttgaaa aggcatgtg aggacgcat 
      acgaaagag cgaccgttt gggtgtcaa tactgtgtt 
      aagaggttt tcctcgaag aatgaatta gttaatcat 
      gagatggtg catcttggg atacgaccg tatcagtgt 
      aaatattgt gagaggtgt tttactaaa tcggttaat 
      ttggatacg cattatttg acacattcg gggccgcat 
      gagtgtcag tattgtgtt aagacgttt cttgaaatt 
      cggtttttg aagatgcat ctgaagagg gtgcataag 
      gagttggaa gttatgact gacgaagtg gaaaattaa
    mRNA sequence
    • >TC015256 RA
      cgattgcaa gcgcctgtg tcaccgata actcaataa 
      agaattgtt tcagaacta catgaaaca attatgcga 
      aggcctttc ctatttaca cacccagcg catccttca 
      ccgtgatat caagcccca aaacttgct agtcgataa 
      agaaggcca catcaaaat cgccgactt tggcctctc 
      tcgctgttt ttccatacc aacaaaacc gtacaccca 
      cgaagtggt gacaatgtg gtacagagc gcctgaact 
      gctcctagg ttccaagct gtacaccaa tgggatcga 
      cgtgtggag tcttgggtg tgttatggt agaaatgtt 
      gttaaaacg tgccctttt ccccggtga ctcagaaat 
      cgaccaaat gttcaaaat tttcaaaac tctgggaac 
      gccaaatga ggaaatgtg gccgggggt gtcgtcact 
      gccggccta tgaggtctg gttcccgca gtggaagcc 
      atgcgagct taacactca aattaaatt caacgattc 
      ggaggaaga agagtttct taaattgtt gttggttta 
      tgatccgtt tgtgcgaaa gtcggccaa agatttgct 
      aagtctgcc ctatatcaa gagagccaa gctcacgac 
      gcctgatat gaagccgtt tcctgacga agaggagga 
      ctctgaact tttaaaatg tgaggttct atcaacaag 
      tcaaagtgt ttttggtgt tttgccttc aatggaatg 
      ttttattag ctccaaaaa cgtggtcaa gttgtgccg 
      tatttgcct cacatcttc aaacaacct actatcatt 
      ggactctcc agtcactgg aaacaacaa cgatttcgc 
      aaactccat acaccacct cattgaaac cgtaacttt 
      ggaaaaagt catagatga cacactact accatcgaa 
      aatttgccc aatatgtct cagctactt gaaaatagc 
      gttcgaatt tcaaacaca gttcaaaaa gtcgcagga 
      aattttgca caaaacgct aaaaattga ggtaccacc 
      tagaaataa caacacgtc ggtggtgca acaacctgt 
      gtccacacc cactgttga attaatctg tggtgagag 
      taaattcga tttgaacga tattatcat aattgagga 
      gaatcaagc cgaccggac gtctttcca aggcttttt 
      gagtaactt aggtacgga aatatcggc ctcattcct 
      tggcgaaag ccacaaaaa tgttcaaat tcccgaacc 
      agttgaaaa cgacgtagt tatattacc aataaaaca 
      cgaacaaaa aatagcaga cgtggaaca agatgtgct 
      tgatatcag tcgcttgag cgtaacaaa agtggccga 
      agagagcaa agttataat aaaaaacaa taacaaagt 
      cgtaaaaac actgtacga gtgtcagac gtgtaaaaa 
      aattcttcc aaatcggca acaattaaa ctttcactt 
      ttcaaccca tgcaaagcg aacgtgtta cgtttgtga 
      caaatgtgg acacattgc caagacacg gtctgcctt 
      ccgccacca tttagccaa acacagtgg ggttgtgtt 
      caagtgcga gtattgcgg gaagtgctt tgacggtaa 
      atcgcactt taaaatcca cgtggaagc gcacaaaaa 
      caaccggcc gtttttgtg caacgtctg tgggaaaac 
      cttcaatta caacacgtc gctcatcta ccacatgag 
      gatccacac aggggagaa gaagtacgt ttgcagctt 
      ctgtggctt gagataccg tatggggac gacgttgaa 
      aaggcatgt gaggacgca tacgaaaga gcgaccgtt 
      tgggtgtca atactgtgt taagaggtt ttcctcgaa 
      gaatgaatt agttaatca tgagatggt gcatcttgg 
      gatacgacc gtatcagtg taaatattg tgagaggtg 
      ttttactaa atcggttaa tttggatac gcattattt 
      gacacattc ggggccgca tgagtgtca gtattgtgt 
      taagacgtt tcttgaaat tcggttttt gaagatgca 
      tctgaagag ggtgcataa ggagttgga agttatgac 
      tgacgaagt ggaaaatta agtcttgta cgcataaaa 
      tgttttttt aatttttcc gttgtttat tttaatccc 
      ttcctatgg aaaatgatc cggggaatc gattggcgt 
      cgagaaaat cgccaaatt cccaatagt tttttagtt 
      acgaatttt tttaaattt taccaattt ttgcattta 
      tctgcaatt tgctcaaaa actattagt cggagaaca 
      ataattttt tttggaaaa aatagataa ttcaaagag 
      ctttccaac gataataaa cttcatggg gttat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY