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Gene information
General information
TC015245 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid56: 2378612..2391578
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015245
    iBeetle screen iB_02439
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015245 RA
      MKTLWTLVL CLASVSPLR ADSVRELSR EARQTQYKS 
      NFRCPSEGF HADPQNCQI FYRCVGTPN NLKPIQFEC 
      GEGTVFDPA ISTCNYPYA SNREECGGN GVDGDFGNV 
      QNPPPSTTT ALPPWTTQS DSTPATVSS QVGDKCTQE 
      GFLGDSRNC SKFYRCVSN GRNGYIQHE FNCGTGTVW 
      DPVAVSCNY PWAAQRSDC SEGQQGSSS NNQQSSSSN 
      QQNQQGSSS SQQGSSSSG SWNQQGGSS SNQQQGSSS 
      SNNFNQQGS NQQGSSSSQ QNQQSSGSS SSQHGSSSG 
      SQNQQSSGS ANQQQSGSS SSQQTGSST SQQGSASSQ 
      NQQSGSLTQ QNQGSSSSN QQSQQGGSS SSSQQNQNQ 
      QGMHRPVNK IIKINKEAI LGTNKGVVL INKAVHRQV 
      NKIKTNKEV ILGINKGVV LLINKAVHY PVSKTKINK 
      EITLGINKG AVLANKIKI NKEAILGIN NKAVLPVNK 
      AAVLRINKT NKVVGRGIS KAVALQINK EAVPPISKI 
      NKVVVLPHN KAVINPINK EAVHLVNKT NKGEISKVL 
      VNKAQISKD RANKETINQ VSKVKFPIM NAYLKVLSK 
      MIMTAKSST DALDILINP ANHNNQASH NNLDIPINP 
      ANQVNHKNR DILISPDNL VNQDSQDNH NNRDPQLEN 
      LNNRAIQDN LNNLDNPVS QVNLVSQGN PVSQVKQVL 
      QNNRVLISK DKQTSRVNQ DSQDNQDNQ GNQDNPGNN 
      QVNQDSQGS REDRDIQIN QDNPGNSKV YQDNLVNLV 
      NKDILINQG KQGRQPGQP GYPNQPGQP GQPGRPGQP 
      GYPNQPGQP GQPGQPGHP NQPGQPGQP GYPNQPGQP 
      GQPGYPNQP GQPGQPGQP NQPGQQGSQ QPQGPNGST 
      SSPVSSQIG DTCTQEGFL ADTKDCKKF YRCVDNGKG 
      SFTKYEFNC GPNTVWDPD AITCNYPYA VNREMCRNY 
      VEQPQSTSP SNCTDCSSQ TGTTPGGTP SGTDGSTTG 
      SVQTTPGST IPDNSTPGS TITESTPSN TITESTTAP 
      STIIDGSTP GTIFTESTT IGTDSTPQG AGTGTTPGA 
      GTTGGTYPG TTGGTYPGT TQGAGTTGG TYPGTFPGT 
      GTTGGVDGT PGTNEPTTT VQSTIGGES TTTDSSQST 
      TAPCPIGQL EGDQIALVC PTGFKRHPK YCNLFYQCT 
      KDADNHDFK ILVLSCPEG RIYDDARIQ CLPQNETSS 
      CSGQIAQSS FYKRLNANS LPPIPVNSE RTLCPSVGF 
      HETDGAVCS TTFVKCQRS YNNFYGKME GYMYQCPQG 
      YVYWAISKQ CERPSRLQH CSGYDIRDT RWEIPVETT 
      NVSLRRRKS ASLFDTIL
    • >TC015245 RB
      MKTLWTLVL CLASVSPLR ADSVRELSR EARQTQYKS 
      NFRCPSEGF HADPQNCQI FYRCVGTPN NLKPIQFEC 
      GEGTVFDPA ISTCNYPYA SNREECGGN GVDGDFGNV 
      QNPPPSTTT ALPPWTTQS DSTPATVSS QVGDKCTQE 
      GFLGDSRNC SKFYRCVSN GRNGYIQHE FNCGTGTVW 
      DPVAVSCNY PWAAQRSDC SEGTSQSFS ALPPQSDYV 
      AQNYNQSNS SQFMSSSSN SSSSSSSST SSSWSQSSS 
      SSSSSTMSS TSSWQNQQN AASSGQQGS SSNNQQSSS 
      SNQQNQQGS SSSQQGSSS SGSWNQQGG SSSNQQQGS 
      SSSNNFNQQ GSNQQGSSS SQQNQQSSG SSSSQHGSS 
      SGSQNQQSS GSANQQQSG SSSSQQTGS STSQQGSAS 
      SQNQQSGSL TQQNQGSSS SNQQSQQGG SSSSSQQNQ 
      NQQGMHRPV NKIIKINKE AILGTNKGV VLINKAVHR 
      QVNKIKTNK EVILGINKG VVLLINKAV HYPVSKTKI 
      NKEITLGIN KGAVLANKI KINKEAILG INNKAVLPV 
      NKAAVLRIN KTNKVVGRG ISKAVALQI NKEAVPPIS 
      KINKVVVLP HNKAVINPI NKEAVHLVN KTNKGEISK 
      VLVNKAQIS KDRANKETI NQVSKVKFP IMNAYLKVL 
      SKMIMTAKS STDALDILI NPANHNNQA SHNNLDIPI 
      NPANQVNHK NRDILISPD NLVNQDSQD NHNNRDPQL 
      ENLNNRAIQ DNLNNLDNP VSQVNLVSQ GNPVSQVKQ 
      VLQNNRVLI SKDKQTSRV NQDSQDNQD NQGNQDNPG 
      NNQVNQDSQ GSREDRDIQ INQDNPGNS KVYQDNLVN 
      LVNKDILIN QGKQGRQPG QPGYPNQPG QPGQPGRPG 
      QPGYPNQPG QPGQPGQPG HPNQPGQPG QPGYPNQPG 
      QPGQPGYPN QPGQPGQPG QPNQPGQQG SQQPQGPNG 
      STSSPVSSQ IGDTCTQEG FLADTKDCK KFYRCVDNG 
      KGSFTKYEF NCGPNTVWD PDAITCNYP YAVNREMCR 
      NYVEQPQST SPSNCTDCS SQTGTTPGG TPSGTDGST 
      TGSVQTTPG STIPDNSTP GSTITESTP SNTITESTT 
      APSTIIDGS TPGTIFTES TTIGTDSTP QGAGTGTTP 
      GAGTTGGTY PGTTGGTYP GTTQGAGTT GGTYPGTFP 
      GTGTTGGVD GTPGTNEPT TTVQSTIGG ESTTTDSSQ 
      STTAPCPIG QLEGDQIAL VCPTGFKRH PKYCNLFYQ 
      CTKDADNHD FKILVLSCP EGRIYDDAR IQCLPQNET 
      SSCSGQIAQ SSFYKRLNA NSLPPIPVN SERTLCPSV 
      GFHETDGAV CSTTFVKCQ RSYNNFYGK MEGYMYQCP 
      QGYVYWAIS KQCERPSRL QHCSGYDIR DTRWEIPVE 
      TTNVSLRRR KSASLFDTI L
    CDS sequence
    • >TC015245 RA
      atgaagact ttgtggacg ttggtcttg tgtctggcg 
      tcggtgtca ccgcttcga gctgactcc gttcgagag 
      ttgtcgaga gaagcacgc caaacgcaa tataaatcg 
      aacttcagg tgtcccagc gaagggttt catgccgat 
      ccgcaaaac tgccaaatt ttttaccga tgtgttggc 
      actccgaat aatctaaag ccgattcag tttgagtgt 
      ggcgaagga actgttttt gatccggcc ataagtact 
      tgcaattac ccctatgct agtaatagg gaggagtgt 
      ggaggaaat ggcgttgat ggtgatttt ggcaacgtt 
      cagaaccct ccaccttca accacaact gcgttacca 
      ccatggacg actcaatca gattcgact cccgcaact 
      gtttctagt caagttggt gacaagtgt acgcaagaa 
      ggcttcttg ggcgatagc agaaactgt tcaaaattc 
      taccgttgt gtcagtaat gggcgcaat ggctacatt 
      cagcatgaa tttaactgt ggtactgga actgtttgg 
      gatccagta gccgtgtct tgtaattac ccatgggca 
      gctcaaaga agtgactgt tcggaaggt caacaggga 
      agcagttca aacaatcaa caaagtagc tcgtcaaat 
      caacaaaat caacaaggg agtagttcc agtcaacaa 
      ggaagctct tcgagcggt agttggaac caacaagga 
      ggcagttca tcaaatcaa cagcaagga agttcgtca 
      agcaataat ttcaatcag caaggttca aatcaacaa 
      ggaagctca tcaagtcaa cagaatcaa caatcatca 
      ggtagtagt tccagtcaa cacggaagt tcttcaggc 
      agtcaaaat caacaaagc agtggttct gccaatcaa 
      caacaaagc ggtagctcg tctagtcaa caaactggt 
      agctctacc agtcaacag ggaagcgcg agcagtcaa 
      aatcaacaa agcggtagt ttaactcaa caaaatcaa 
      ggaagtagc tcatcgaac caacaaagt caacaaggt 
      ggtagttca tcatccagt caacaaaac caaaatcaa 
      caaggaatg catcgtcca gtcaacaaa ataatcaaa 
      atcaacaag gaagcaatt cttggaacc aacaagggg 
      gtagttcta atcaacaag gcagtacat cgtcaagtc 
      aacaaaatc aaaaccaac aaggaagta attcttgga 
      atcaacaag ggggtagtt cttctaatc aacaaggca 
      gtacattat ccagtcagc aaaaccaaa atcaacaag 
      gaaataact cttggaatc aacaagggg gcagttcta 
      gccaacaaa atcaaaatc aacaaggaa gcaattctt 
      ggaatcaac aacaaggcg gttcttcca gtcaacaag 
      gcggcagtt cttcgaatc aacaaaacc aacaaagtg 
      gtgggtcgt ggaatcagc aaggcggta gcacttcaa 
      atcaacaag gaagcagtt cctccaatc agcaaaatc 
      aacaaggtg gtagttctt ccacacaac aaggcagta 
      atcaatcca atcaacaag gaagcagtt catctagtc 
      aacaaaacc aacaagggg gaaatcagc aaggttcta 
      gtcaacaag gcacaaatc agcaaggat cgagccaac 
      aaggaaaca atcaatcaa gtcagcaag gtcaaattt 
      ccaataatg aatgcgtat ctgaaggtt ttgtcaaag 
      atgataatg actgcaaaa agttctaca gatgccctg 
      gatatccta atcaacccg gccaaccac aacaaccag 
      gccagccac aacaatctg gatatccca atcaacccg 
      gccaaccag gtcaaccac aaaaaccgg gatatccta 
      atcagcccg gacaacctg gtcaaccag gacagccag 
      gacaaccac aacaaccgg gatccccaa ctggaaaac 
      ctcaacaac cgagctatc caggacaac ctcaacaac 
      ctggacaat ccggtcagc caggtcaac ctggtcagc 
      cagggcaac ccggtcagt caggtcaaa caagtactc 
      cagaacaac agggtccta atcagcaag gacaaacaa 
      accagtcgg gtcaaccag gacagccag gacaaccag 
      gacaaccag ggcaatcag gacaacccg ggcaacaac 
      caggtcaac caggacagc cagggcagc cgggaagac 
      cgggatatc caaatcaac caggacaac ccgggcaac 
      agcaaggtt taccaggac aacctggtc aacctagtc 
      aacaaggac atcctaatc aaccagggc aaacagggc 
      agacagcca ggacaacca ggatatccc aatcagccg 
      gggcagcct ggtcaacca ggacgacca ggacaacca 
      ggatatcct aatcaaccg gggcagcct ggtcaacca 
      gggcaacca ggacatcct aatcaaccg ggacagcct 
      ggtcaacca ggttatccc aaccaacca ggacagcca 
      gggcaacct ggatatcct aatcaaccg ggacagcct 
      ggtcaacct gggcagcct aaccaacca ggccaacaa 
      gggagtcaa caacctcag ggaccaaat ggctccaca 
      tcatcacca gtttcaagt caaattgga gacacttgc 
      actcaagaa ggtttcctg gctgacaca aaagattgt 
      aaaaaattc tacagatgc gtagataat ggaaagggc 
      agttttaca aaatatgaa ttcaactgc ggtccaaac 
      acggtttgg gatccagac gcaataact tgcaactat 
      ccgtatgct gttaatcgg gaaatgtgt cgaaattac 
      gtagaacaa ccacaaagc acatctccc tccaactgc 
      acagactgt agttctcaa actggaact accccaggt 
      ggtacaccc tctggcaca gacggttcc actacggga 
      tcagttcaa acgacaccc ggaagcacg ataccagac 
      aatagcaca cctggcagt acaatcaca gaaagcact 
      cctagtaat acaatcaca gaaagcacg actgcccct 
      agcactata attgatggc tctactcca ggcaccatc 
      tttacagaa agtaccaca attggaaca gacagtacc 
      ccacaaggt gctggaacg ggaaccact ccaggtgcg 
      ggaacgact ggcggtacc tatccggga acaactggt 
      ggtacttat ccgggaacg acacagggt gctggaaca 
      acaggcggt acctatccg ggtactttc cccggtact 
      ggaacaaca ggtggcgtt gacggtacc ccaggtacc 
      aacgaacca acaaccaca gtccaaagc acaattggc 
      ggcgaaagt accaccact gattcatct caatccacc 
      acagcccca tgccctatc ggacaatta gaaggcgac 
      caaatcgct ctagtttgc ccaaccgga ttcaaaaga 
      catccaaaa tactgcaat ctattctac caatgtacg 
      aaagacgca gacaatcac gactttaaa atcctcgtt 
      ttgagttgc cctgaagga aggatttac gacgacgcc 
      cgcatccag tgcttgcct caaaacgaa acaagttct 
      tgcagcgga caaatcgct caaagctcc ttctacaag 
      cgacttaac gccaattca ttgcctccg atacccgtc 
      aacagcgaa cggacgctc tgccccagt gtcggcttc 
      cacgaaacc gatggcgcc gtttgctca acaacgttt 
      gtcaaatgc cagcgctct tacaataac ttctatgga 
      aaaatggaa ggttacatg taccagtgc ccacaagga 
      tacgtttat tgggcgata agtaaacag tgcgaacgc 
      ccatccaga ttgcaacac tgcagcggc tacgacatc 
      cgcgacaca agatgggag ataccagta gagaccaca 
      aatgtctca ttaagacga agaaaaagt gccagttta 
      ttcgatacg atcctttaa
    • >TC015245 RB
      atgaagact ttgtggacg ttggtcttg tgtctggcg 
      tcggtgtca ccgcttcga gctgactcc gttcgagag 
      ttgtcgaga gaagcacgc caaacgcaa tataaatcg 
      aacttcagg tgtcccagc gaagggttt catgccgat 
      ccgcaaaac tgccaaatt ttttaccga tgtgttggc 
      actccgaat aatctaaag ccgattcag tttgagtgt 
      ggcgaagga actgttttt gatccggcc ataagtact 
      tgcaattac ccctatgct agtaatagg gaggagtgt 
      ggaggaaat ggcgttgat ggtgatttt ggcaacgtt 
      cagaaccct ccaccttca accacaact gcgttacca 
      ccatggacg actcaatca gattcgact cccgcaact 
      gtttctagt caagttggt gacaagtgt acgcaagaa 
      ggcttcttg ggcgatagc agaaactgt tcaaaattc 
      taccgttgt gtcagtaat gggcgcaat ggctacatt 
      cagcatgaa tttaactgt ggtactgga actgtttgg 
      gatccagta gccgtgtct tgtaattac ccatgggca 
      gctcaaaga agtgactgt tcggaaggt actagtcag 
      agtttttcc gcactgcct ccacagagt gattatgta 
      gcccaaaac tataaccag tctaacagt agtcagttt 
      atgagtagt agtagcaat agcagtagt agtagcagt 
      agcagtacg agtagttcc tggagccag tctagttct 
      tcgtcgtct tcctctact atgtcaagc acgagttct 
      tggcaaaat caacaaaat gctgcttcc tcaggtcaa 
      cagggaagc agttcaaac aatcaacaa agtagctcg 
      tcaaatcaa caaaatcaa caagggagt agttccagt 
      caacaagga agctcttcg agcggtagt tggaaccaa 
      caaggaggc agttcatca aatcaacag caaggaagt 
      tcgtcaagc aataatttc aatcagcaa ggttcaaat 
      caacaagga agctcatca agtcaacag aatcaacaa 
      tcatcaggt agtagttcc agtcaacac ggaagttct 
      tcaggcagt caaaatcaa caaagcagt ggttctgcc 
      aatcaacaa caaagcggt agctcgtct agtcaacaa 
      actggtagc tctaccagt caacaggga agcgcgagc 
      agtcaaaat caacaaagc ggtagttta actcaacaa 
      aatcaagga agtagctca tcgaaccaa caaagtcaa 
      caaggtggt agttcatca tccagtcaa caaaaccaa 
      aatcaacaa ggaatgcat cgtccagtc aacaaaata 
      atcaaaatc aacaaggaa gcaattctt ggaaccaac 
      aagggggta gttctaatc aacaaggca gtacatcgt 
      caagtcaac aaaatcaaa accaacaag gaagtaatt 
      cttggaatc aacaagggg gtagttctt ctaatcaac 
      aaggcagta cattatcca gtcagcaaa accaaaatc 
      aacaaggaa ataactctt ggaatcaac aagggggca 
      gttctagcc aacaaaatc aaaatcaac aaggaagca 
      attcttgga atcaacaac aaggcggtt cttccagtc 
      aacaaggcg gcagttctt cgaatcaac aaaaccaac 
      aaagtggtg ggtcgtgga atcagcaag gcggtagca 
      cttcaaatc aacaaggaa gcagttcct ccaatcagc 
      aaaatcaac aaggtggta gttcttcca cacaacaag 
      gcagtaatc aatccaatc aacaaggaa gcagttcat 
      ctagtcaac aaaaccaac aagggggaa atcagcaag 
      gttctagtc aacaaggca caaatcagc aaggatcga 
      gccaacaag gaaacaatc aatcaagtc agcaaggtc 
      aaatttcca ataatgaat gcgtatctg aaggttttg 
      tcaaagatg ataatgact gcaaaaagt tctacagat 
      gccctggat atcctaatc aacccggcc aaccacaac 
      aaccaggcc agccacaac aatctggat atcccaatc 
      aacccggcc aaccaggtc aaccacaaa aaccgggat 
      atcctaatc agcccggac aacctggtc aaccaggac 
      agccaggac aaccacaac aaccgggat ccccaactg 
      gaaaacctc aacaaccga gctatccag gacaacctc 
      aacaacctg gacaatccg gtcagccag gtcaacctg 
      gtcagccag ggcaacccg gtcagtcag gtcaaacaa 
      gtactccag aacaacagg gtcctaatc agcaaggac 
      aaacaaacc agtcgggtc aaccaggac agccaggac 
      aaccaggac aaccagggc aatcaggac aacccgggc 
      aacaaccag gtcaaccag gacagccag ggcagccgg 
      gaagaccgg gatatccaa atcaaccag gacaacccg 
      ggcaacagc aaggtttac caggacaac ctggtcaac 
      ctagtcaac aaggacatc ctaatcaac cagggcaaa 
      cagggcaga cagccagga caaccagga tatcccaat 
      cagccgggg cagcctggt caaccagga cgaccagga 
      caaccagga tatcctaat caaccgggg cagcctggt 
      caaccaggg caaccagga catcctaat caaccggga 
      cagcctggt caaccaggt tatcccaac caaccagga 
      cagccaggg caacctgga tatcctaat caaccggga 
      cagcctggt caacctggg cagcctaac caaccaggc 
      caacaaggg agtcaacaa cctcaggga ccaaatggc 
      tccacatca tcaccagtt tcaagtcaa attggagac 
      acttgcact caagaaggt ttcctggct gacacaaaa 
      gattgtaaa aaattctac agatgcgta gataatgga 
      aagggcagt tttacaaaa tatgaattc aactgcggt 
      ccaaacacg gtttgggat ccagacgca ataacttgc 
      aactatccg tatgctgtt aatcgggaa atgtgtcga 
      aattacgta gaacaacca caaagcaca tctccctcc 
      aactgcaca gactgtagt tctcaaact ggaactacc 
      ccaggtggt acaccctct ggcacagac ggttccact 
      acgggatca gttcaaacg acacccgga agcacgata 
      ccagacaat agcacacct ggcagtaca atcacagaa 
      agcactcct agtaataca atcacagaa agcacgact 
      gcccctagc actataatt gatggctct actccaggc 
      accatcttt acagaaagt accacaatt ggaacagac 
      agtacccca caaggtgct ggaacggga accactcca 
      ggtgcggga acgactggc ggtacctat ccgggaaca 
      actggtggt acttatccg ggaacgaca cagggtgct 
      ggaacaaca ggcggtacc tatccgggt actttcccc 
      ggtactgga acaacaggt ggcgttgac ggtacccca 
      ggtaccaac gaaccaaca accacagtc caaagcaca 
      attggcggc gaaagtacc accactgat tcatctcaa 
      tccaccaca gccccatgc cctatcgga caattagaa 
      ggcgaccaa atcgctcta gtttgccca accggattc 
      aaaagacat ccaaaatac tgcaatcta ttctaccaa 
      tgtacgaaa gacgcagac aatcacgac tttaaaatc 
      ctcgttttg agttgccct gaaggaagg atttacgac 
      gacgcccgc atccagtgc ttgcctcaa aacgaaaca 
      agttcttgc agcggacaa atcgctcaa agctccttc 
      tacaagcga cttaacgcc aattcattg cctccgata 
      cccgtcaac agcgaacgg acgctctgc cccagtgtc 
      ggcttccac gaaaccgat ggcgccgtt tgctcaaca 
      acgtttgtc aaatgccag cgctcttac aataacttc 
      tatggaaaa atggaaggt tacatgtac cagtgccca 
      caaggatac gtttattgg gcgataagt aaacagtgc 
      gaacgccca tccagattg caacactgc agcggctac 
      gacatccgc gacacaaga tgggagata ccagtagag 
      accacaaat gtctcatta agacgaaga aaaagtgcc 
      agtttattc gatacgatc ctttaa
    mRNA sequence
    • >TC015245 RA
      accacccga aggataatt cagtggcca ggaaggaag 
      gagtgcacc gaaaaacgc gctttccag gagatgatc 
      cgaacgagt tccaattag ggaattctc aagaacaca 
      atgaagact ttgtggacg ttggtcttg tgtctggcg 
      tcggtgtca ccgcttcga gctgactcc gttcgagag 
      ttgtcgaga gaagcacgc caaacgcaa tataaatcg 
      aacttcagg tgtcccagc gaagggttt catgccgat 
      ccgcaaaac tgccaaatt ttttaccga tgtgttggc 
      actccgaat aatctaaag ccgattcag tttgagtgt 
      ggcgaagga actgttttt gatccggcc ataagtact 
      tgcaattac ccctatgct agtaatagg gaggagtgt 
      ggaggaaat ggcgttgat ggtgatttt ggcaacgtt 
      cagaaccct ccaccttca accacaact gcgttacca 
      ccatggacg actcaatca gattcgact cccgcaact 
      gtttctagt caagttggt gacaagtgt acgcaagaa 
      ggcttcttg ggcgatagc agaaactgt tcaaaattc 
      taccgttgt gtcagtaat gggcgcaat ggctacatt 
      cagcatgaa tttaactgt ggtactgga actgtttgg 
      gatccagta gccgtgtct tgtaattac ccatgggca 
      gctcaaaga agtgactgt tcggaaggt caacaggga 
      agcagttca aacaatcaa caaagtagc tcgtcaaat 
      caacaaaat caacaaggg agtagttcc agtcaacaa 
      ggaagctct tcgagcggt agttggaac caacaagga 
      ggcagttca tcaaatcaa cagcaagga agttcgtca 
      agcaataat ttcaatcag caaggttca aatcaacaa 
      ggaagctca tcaagtcaa cagaatcaa caatcatca 
      ggtagtagt tccagtcaa cacggaagt tcttcaggc 
      agtcaaaat caacaaagc agtggttct gccaatcaa 
      caacaaagc ggtagctcg tctagtcaa caaactggt 
      agctctacc agtcaacag ggaagcgcg agcagtcaa 
      aatcaacaa agcggtagt ttaactcaa caaaatcaa 
      ggaagtagc tcatcgaac caacaaagt caacaaggt 
      ggtagttca tcatccagt caacaaaac caaaatcaa 
      caaggaatg catcgtcca gtcaacaaa ataatcaaa 
      atcaacaag gaagcaatt cttggaacc aacaagggg 
      gtagttcta atcaacaag gcagtacat cgtcaagtc 
      aacaaaatc aaaaccaac aaggaagta attcttgga 
      atcaacaag ggggtagtt cttctaatc aacaaggca 
      gtacattat ccagtcagc aaaaccaaa atcaacaag 
      gaaataact cttggaatc aacaagggg gcagttcta 
      gccaacaaa atcaaaatc aacaaggaa gcaattctt 
      ggaatcaac aacaaggcg gttcttcca gtcaacaag 
      gcggcagtt cttcgaatc aacaaaacc aacaaagtg 
      gtgggtcgt ggaatcagc aaggcggta gcacttcaa 
      atcaacaag gaagcagtt cctccaatc agcaaaatc 
      aacaaggtg gtagttctt ccacacaac aaggcagta 
      atcaatcca atcaacaag gaagcagtt catctagtc 
      aacaaaacc aacaagggg gaaatcagc aaggttcta 
      gtcaacaag gcacaaatc agcaaggat cgagccaac 
      aaggaaaca atcaatcaa gtcagcaag gtcaaattt 
      ccaataatg aatgcgtat ctgaaggtt ttgtcaaag 
      atgataatg actgcaaaa agttctaca gatgccctg 
      gatatccta atcaacccg gccaaccac aacaaccag 
      gccagccac aacaatctg gatatccca atcaacccg 
      gccaaccag gtcaaccac aaaaaccgg gatatccta 
      atcagcccg gacaacctg gtcaaccag gacagccag 
      gacaaccac aacaaccgg gatccccaa ctggaaaac 
      ctcaacaac cgagctatc caggacaac ctcaacaac 
      ctggacaat ccggtcagc caggtcaac ctggtcagc 
      cagggcaac ccggtcagt caggtcaaa caagtactc 
      cagaacaac agggtccta atcagcaag gacaaacaa 
      accagtcgg gtcaaccag gacagccag gacaaccag 
      gacaaccag ggcaatcag gacaacccg ggcaacaac 
      caggtcaac caggacagc cagggcagc cgggaagac 
      cgggatatc caaatcaac caggacaac ccgggcaac 
      agcaaggtt taccaggac aacctggtc aacctagtc 
      aacaaggac atcctaatc aaccagggc aaacagggc 
      agacagcca ggacaacca ggatatccc aatcagccg 
      gggcagcct ggtcaacca ggacgacca ggacaacca 
      ggatatcct aatcaaccg gggcagcct ggtcaacca 
      gggcaacca ggacatcct aatcaaccg ggacagcct 
      ggtcaacca ggttatccc aaccaacca ggacagcca 
      gggcaacct ggatatcct aatcaaccg ggacagcct 
      ggtcaacct gggcagcct aaccaacca ggccaacaa 
      gggagtcaa caacctcag ggaccaaat ggctccaca 
      tcatcacca gtttcaagt caaattgga gacacttgc 
      actcaagaa ggtttcctg gctgacaca aaagattgt 
      aaaaaattc tacagatgc gtagataat ggaaagggc 
      agttttaca aaatatgaa ttcaactgc ggtccaaac 
      acggtttgg gatccagac gcaataact tgcaactat 
      ccgtatgct gttaatcgg gaaatgtgt cgaaattac 
      gtagaacaa ccacaaagc acatctccc tccaactgc 
      acagactgt agttctcaa actggaact accccaggt 
      ggtacaccc tctggcaca gacggttcc actacggga 
      tcagttcaa acgacaccc ggaagcacg ataccagac 
      aatagcaca cctggcagt acaatcaca gaaagcact 
      cctagtaat acaatcaca gaaagcacg actgcccct 
      agcactata attgatggc tctactcca ggcaccatc 
      tttacagaa agtaccaca attggaaca gacagtacc 
      ccacaaggt gctggaacg ggaaccact ccaggtgcg 
      ggaacgact ggcggtacc tatccggga acaactggt 
      ggtacttat ccgggaacg acacagggt gctggaaca 
      acaggcggt acctatccg ggtactttc cccggtact 
      ggaacaaca ggtggcgtt gacggtacc ccaggtacc 
      aacgaacca acaaccaca gtccaaagc acaattggc 
      ggcgaaagt accaccact gattcatct caatccacc 
      acagcccca tgccctatc ggacaatta gaaggcgac 
      caaatcgct ctagtttgc ccaaccgga ttcaaaaga 
      catccaaaa tactgcaat ctattctac caatgtacg 
      aaagacgca gacaatcac gactttaaa atcctcgtt 
      ttgagttgc cctgaagga aggatttac gacgacgcc 
      cgcatccag tgcttgcct caaaacgaa acaagttct 
      tgcagcgga caaatcgct caaagctcc ttctacaag 
      cgacttaac gccaattca ttgcctccg atacccgtc 
      aacagcgaa cggacgctc tgccccagt gtcggcttc 
      cacgaaacc gatggcgcc gtttgctca acaacgttt 
      gtcaaatgc cagcgctct tacaataac ttctatgga 
      aaaatggaa ggttacatg taccagtgc ccacaagga 
      tacgtttat tgggcgata agtaaacag tgcgaacgc 
      ccatccaga ttgcaacac tgcagcggc tacgacatc 
      cgcgacaca agatgggag ataccagta gagaccaca 
      aatgtctca ttaagacga agaaaaagt gccagttta 
      ttcgatacg atcctttaa gtaaatctc gttgccagc 
      tagtttgta agtagtact cgcaagtac caccgatcg 
      agataagga ttgtttagt acttgtcga gttagtaat 
      gtactataa cactccatt tagactgat acgaactaa 
      ttcgatttt ctttgaact aaaccactt agttcaaag 
      caaaatgtg tctttaatg cagcacagc tttttcaca 
      gtttcgcgc aaactgtgg aaatgccgt cattcggga 
      aggttctgg gtttattta tttcaattt atttattta 
      gtaaaattt ggcaccgaa atttgctgt caaacgaac 
      gatctagtt tacttaagc atcgattac attgtatac 
      tgaatgtaa taatttttc aatctcaat aaattttac 
      tataattg
    • >TC015245 RB
      accacccga aggataatt cagtggcca ggaaggaag 
      gagtgcacc gaaaaacgc gctttccag gagatgatc 
      cgaacgagt tccaattag ggaattctc aagaacaca 
      atgaagact ttgtggacg ttggtcttg tgtctggcg 
      tcggtgtca ccgcttcga gctgactcc gttcgagag 
      ttgtcgaga gaagcacgc caaacgcaa tataaatcg 
      aacttcagg tgtcccagc gaagggttt catgccgat 
      ccgcaaaac tgccaaatt ttttaccga tgtgttggc 
      actccgaat aatctaaag ccgattcag tttgagtgt 
      ggcgaagga actgttttt gatccggcc ataagtact 
      tgcaattac ccctatgct agtaatagg gaggagtgt 
      ggaggaaat ggcgttgat ggtgatttt ggcaacgtt 
      cagaaccct ccaccttca accacaact gcgttacca 
      ccatggacg actcaatca gattcgact cccgcaact 
      gtttctagt caagttggt gacaagtgt acgcaagaa 
      ggcttcttg ggcgatagc agaaactgt tcaaaattc 
      taccgttgt gtcagtaat gggcgcaat ggctacatt 
      cagcatgaa tttaactgt ggtactgga actgtttgg 
      gatccagta gccgtgtct tgtaattac ccatgggca 
      gctcaaaga agtgactgt tcggaaggt actagtcag 
      agtttttcc gcactgcct ccacagagt gattatgta 
      gcccaaaac tataaccag tctaacagt agtcagttt 
      atgagtagt agtagcaat agcagtagt agtagcagt 
      agcagtacg agtagttcc tggagccag tctagttct 
      tcgtcgtct tcctctact atgtcaagc acgagttct 
      tggcaaaat caacaaaat gctgcttcc tcaggtcaa 
      cagggaagc agttcaaac aatcaacaa agtagctcg 
      tcaaatcaa caaaatcaa caagggagt agttccagt 
      caacaagga agctcttcg agcggtagt tggaaccaa 
      caaggaggc agttcatca aatcaacag caaggaagt 
      tcgtcaagc aataatttc aatcagcaa ggttcaaat 
      caacaagga agctcatca agtcaacag aatcaacaa 
      tcatcaggt agtagttcc agtcaacac ggaagttct 
      tcaggcagt caaaatcaa caaagcagt ggttctgcc 
      aatcaacaa caaagcggt agctcgtct agtcaacaa 
      actggtagc tctaccagt caacaggga agcgcgagc 
      agtcaaaat caacaaagc ggtagttta actcaacaa 
      aatcaagga agtagctca tcgaaccaa caaagtcaa 
      caaggtggt agttcatca tccagtcaa caaaaccaa 
      aatcaacaa ggaatgcat cgtccagtc aacaaaata 
      atcaaaatc aacaaggaa gcaattctt ggaaccaac 
      aagggggta gttctaatc aacaaggca gtacatcgt 
      caagtcaac aaaatcaaa accaacaag gaagtaatt 
      cttggaatc aacaagggg gtagttctt ctaatcaac 
      aaggcagta cattatcca gtcagcaaa accaaaatc 
      aacaaggaa ataactctt ggaatcaac aagggggca 
      gttctagcc aacaaaatc aaaatcaac aaggaagca 
      attcttgga atcaacaac aaggcggtt cttccagtc 
      aacaaggcg gcagttctt cgaatcaac aaaaccaac 
      aaagtggtg ggtcgtgga atcagcaag gcggtagca 
      cttcaaatc aacaaggaa gcagttcct ccaatcagc 
      aaaatcaac aaggtggta gttcttcca cacaacaag 
      gcagtaatc aatccaatc aacaaggaa gcagttcat 
      ctagtcaac aaaaccaac aagggggaa atcagcaag 
      gttctagtc aacaaggca caaatcagc aaggatcga 
      gccaacaag gaaacaatc aatcaagtc agcaaggtc 
      aaatttcca ataatgaat gcgtatctg aaggttttg 
      tcaaagatg ataatgact gcaaaaagt tctacagat 
      gccctggat atcctaatc aacccggcc aaccacaac 
      aaccaggcc agccacaac aatctggat atcccaatc 
      aacccggcc aaccaggtc aaccacaaa aaccgggat 
      atcctaatc agcccggac aacctggtc aaccaggac 
      agccaggac aaccacaac aaccgggat ccccaactg 
      gaaaacctc aacaaccga gctatccag gacaacctc 
      aacaacctg gacaatccg gtcagccag gtcaacctg 
      gtcagccag ggcaacccg gtcagtcag gtcaaacaa 
      gtactccag aacaacagg gtcctaatc agcaaggac 
      aaacaaacc agtcgggtc aaccaggac agccaggac 
      aaccaggac aaccagggc aatcaggac aacccgggc 
      aacaaccag gtcaaccag gacagccag ggcagccgg 
      gaagaccgg gatatccaa atcaaccag gacaacccg 
      ggcaacagc aaggtttac caggacaac ctggtcaac 
      ctagtcaac aaggacatc ctaatcaac cagggcaaa 
      cagggcaga cagccagga caaccagga tatcccaat 
      cagccgggg cagcctggt caaccagga cgaccagga 
      caaccagga tatcctaat caaccgggg cagcctggt 
      caaccaggg caaccagga catcctaat caaccggga 
      cagcctggt caaccaggt tatcccaac caaccagga 
      cagccaggg caacctgga tatcctaat caaccggga 
      cagcctggt caacctggg cagcctaac caaccaggc 
      caacaaggg agtcaacaa cctcaggga ccaaatggc 
      tccacatca tcaccagtt tcaagtcaa attggagac 
      acttgcact caagaaggt ttcctggct gacacaaaa 
      gattgtaaa aaattctac agatgcgta gataatgga 
      aagggcagt tttacaaaa tatgaattc aactgcggt 
      ccaaacacg gtttgggat ccagacgca ataacttgc 
      aactatccg tatgctgtt aatcgggaa atgtgtcga 
      aattacgta gaacaacca caaagcaca tctccctcc 
      aactgcaca gactgtagt tctcaaact ggaactacc 
      ccaggtggt acaccctct ggcacagac ggttccact 
      acgggatca gttcaaacg acacccgga agcacgata 
      ccagacaat agcacacct ggcagtaca atcacagaa 
      agcactcct agtaataca atcacagaa agcacgact 
      gcccctagc actataatt gatggctct actccaggc 
      accatcttt acagaaagt accacaatt ggaacagac 
      agtacccca caaggtgct ggaacggga accactcca 
      ggtgcggga acgactggc ggtacctat ccgggaaca 
      actggtggt acttatccg ggaacgaca cagggtgct 
      ggaacaaca ggcggtacc tatccgggt actttcccc 
      ggtactgga acaacaggt ggcgttgac ggtacccca 
      ggtaccaac gaaccaaca accacagtc caaagcaca 
      attggcggc gaaagtacc accactgat tcatctcaa 
      tccaccaca gccccatgc cctatcgga caattagaa 
      ggcgaccaa atcgctcta gtttgccca accggattc 
      aaaagacat ccaaaatac tgcaatcta ttctaccaa 
      tgtacgaaa gacgcagac aatcacgac tttaaaatc 
      ctcgttttg agttgccct gaaggaagg atttacgac 
      gacgcccgc atccagtgc ttgcctcaa aacgaaaca 
      agttcttgc agcggacaa atcgctcaa agctccttc 
      tacaagcga cttaacgcc aattcattg cctccgata 
      cccgtcaac agcgaacgg acgctctgc cccagtgtc 
      ggcttccac gaaaccgat ggcgccgtt tgctcaaca 
      acgtttgtc aaatgccag cgctcttac aataacttc 
      tatggaaaa atggaaggt tacatgtac cagtgccca 
      caaggatac gtttattgg gcgataagt aaacagtgc 
      gaacgccca tccagattg caacactgc agcggctac 
      gacatccgc gacacaaga tgggagata ccagtagag 
      accacaaat gtctcatta agacgaaga aaaagtgcc 
      agtttattc gatacgatc ctttaagta aatctcgtt 
      gccagctag tttgtaagt agtactcgc aagtaccac 
      cgatcgaga taaggattg tttagtact tgtcgagtt 
      agtaatgta ctataacac tccatttag actgatacg 
      aactaattc gattttctt tgaactaaa ccacttagt 
      tcaaagcaa aatgtgtct ttaatgcag cacagcttt 
      ttcacagtt tcgcgcaaa ctgtggaaa tgccgtcat 
      tcgggaagg ttctgggtt tatttattt caatttatt 
      tatttagta aaatttggc accgaaatt tgctgtcaa 
      acgaacgat ctagtttac ttaagcatc gattacatt 
      gtatactga atgtaataa tttttcaat ctcaataaa 
      ttttactat aattg