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Gene information
General information
TC015240 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 9401665..9404559
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015240
    iBeetle screen iB_02438

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    cuticle remnants without visible segmentation but polarity - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle potentially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen partially remaining - Penetrance (percentage of animals/eggs on slide): less than 30%

    antenna not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    hindgut protruding - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015240 RA
      MNISPSLSK FLFILILLS VIRGQEENE ELLAEIQLS 
      NVELQDDCE NVANKYKSS IIDNTFTEK ITAQEIHSK 
      LLRTQSETL RPFGDIPYD LQRNWELIV KPGDAILEE 
      PDWSTLVSY EVGIDELIH TFKVHCKNN TKNCVEVKR 
      GYDDLLETS RNATLRKKS WSAWQTLLS TKFNKFHSV 
      LNLINKAAR LNEFDDPKL YWEKLQDLE GAYNLADGF 
      WDQIKPFYT KLQNYIKTR LLRFYKTTS DDIPVYLSG 
      SNFGDDWSS IANIILPHP QIYYYAESY LNQTSIKEI 
      YKMAEQVTK DLALGPLGP KFWANSRFN LPFCENHVV 
      TFCSQEHSE VFTCDKTSW VHYLNSFET AIDVALRSV 
      NYVDLARLK LRYSAIDDA IVGLGSIFA IKNLRVKGV 
      WDYELFQNN DNEMSELLL IALRVLPKL PYYLAADKW 
      RLEVLQNGG GAMTATWWN LRKELQGIG GVFNNETDF 
      LGDSFIVSN RPYLSKFLG TILQFQLLE HYRNGWQYA 
      NESLAANFG NDDNFLTML HDRTSSDWP ALINGFGVY 
      ELSATPLLD YFAPLNDYL DATLNTEQV VPSTTPKPT 
      KAQKIKTLI VKKTNVTET DEDVQNDEP EPEVPRHIL 
      EDREETPNH AMEKITVFI GVGLLAGVA AIIGFVILR 
      KKMTGRGRT NNRRFET
    CDS sequence
    • >TC015240 RA
      atgaatatt tccccaagt ctatcgaaa tttttattt 
      attttgatc ttgctgtcc gtaattcgg ggacaagaa 
      gaaaatgaa gaactgtta gccgaaata caactctcc 
      aatgtagaa ttgcaagac gattgcgaa aatgtagca 
      aataaatac aaatcgtca ataatagac aatactttt 
      acagaaaag attacagca caagaaatt cacagcaaa 
      ttgttacga actcagtct gaaacatta cgacctttt 
      ggcgacatt ccctacgat cttcagaga aactgggaa 
      ttgatcgtc aaacccggc gatgcaatt ctggaagag 
      ccagactgg agtacttta gtatcgtat gaggttggc 
      atcgacgag ttaattcac acttttaaa gttcattgc 
      aaaaacaat acgaaaaat tgtgttgaa gtcaagaga 
      ggctatgat gacttgctc gaaacgtca agaaatgca 
      actttacgg aagaagagt tggtccgct tggcagacg 
      ctactttcg accaaattc aacaaattc cactcggtt 
      ttgaatctc attaacaaa gcggctcga ctgaatgaa 
      tttgacgac cctaagtta tactgggag aagttacaa 
      gatctagaa ggcgcgtat aacttagcc gatggtttc 
      tgggaccaa atcaaacca ttttacacg aagttacaa 
      aattacatc aagacccgg cttttgcgt ttttacaaa 
      acaactagt gacgacatc cctgtgtac ctttcgggt 
      tcaaatttt ggggacgac tggagcagc attgccaac 
      ataatccta ccccatcct caaatctat tactatgcg 
      gagtcttac ctcaaccaa acttcaatc aaagaaatt 
      tacaaaatg gccgagcaa gtcactaag gacttggcc 
      ttaggccct ttgggcccc aaattttgg gccaatagc 
      cgcttcaat ttgcccttc tgtgagaac cacgtggtt 
      acgttctgc tcgcaggaa cattccgaa gtgttcact 
      tgcgataaa acatcatgg gtccactat ttaaactca 
      tttgagacg gcaattgac gtggcgtta cgtagcgtc 
      aattatgtt gatttggca agactgaag ttgcgatat 
      tccgccatt gacgacgcc attgttggt ctcggatcg 
      atttttgct atcaagaat ctgcgagtc aaaggagtc 
      tgggactac gaattgttc caaaacaat gataatgaa 
      atgagcgaa cttttatta attgcactg agagttttg 
      ccgaaactt ccgtactat ctggccgcc gataaatgg 
      cggctggaa gtattgcaa aatggtgga ggcgccatg 
      acggccacg tggtggaac ttgcggaag gagttgcaa 
      ggaattggg ggagttttc aacaacgaa accgatttt 
      cttggcgat tcgttcatt gtttcgaat cggccatat 
      ttgagtaaa ttcctcgga accatcttg cagtttcaa 
      ttgctggaa cattaccga aacgggtgg caatatgcc 
      aacgaaagc cttgcggct aatttcgga aacgacgat 
      aatttctta acaatgttg cacgacagg acgtccagt 
      gattggcca gcattgatt aacggtttt ggggtttac 
      gaattgtcg gcgacccca cttttggat tactttgct 
      ccgctaaat gattatctg gacgcgaca ctgaatacg 
      gaacaagtt gtgccttca acgacccca aagcccaca 
      aaagcccag aaaattaaa actcttatt gttaaaaag 
      acaaacgta acagagaca gatgaagat gtgcaaaat 
      gatgaaccg gagcccgaa gttccgaga catattctt 
      gaagatcgt gaagaaact cccaatcac gccatggaa 
      aaaataaca gtttttata ggagtgggg cttttggcg 
      ggtgtcgct gccattata ggatttgtg atacttagg 
      aaaaaaatg acggggaga ggacggacg aataatagg 
      cgctttgag acttga
    mRNA sequence
    • >TC015240 RA
      aaatacgtg tccaaaatc ttactggaa tgactaagg 
      tcataccat cccaacttt cactcgttt cttcagttt 
      ctcttaaac cgtgacaat gtcagatcg attattcac 
      agaaataag cataaacat gttaattcc gagtgcacg 
      aaaaccgca aatatgaag agataacag cgcaattgt 
      ccccagaat acgaaagtc tagtaacca tcggtagaa 
      atcttatca aggcaatga caatatttt gaccgttgc 
      accaggttt gtttcatga ttcacgagg tttgtgaat 
      agttacaaa tccacaact gtttagagt gcaagctta 
      tttggtcta cttttgact attttcatg taaaactat 
      tgtttttca ctgcccgaa atgaatatt tccccaagt 
      ctatcgaaa tttttattt attttgatc ttgctgtcc 
      gtaattcgg ggacaagaa gaaaatgaa gaactgtta 
      gccgaaata caactctcc aatgtagaa ttgcaagac 
      gattgcgaa aatgtagca aataaatac aaatcgtca 
      ataatagac aatactttt acagaaaag attacagca 
      caagaaatt cacagcaaa ttgttacga actcagtct 
      gaaacatta cgacctttt ggcgacatt ccctacgat 
      cttcagaga aactgggaa ttgatcgtc aaacccggc 
      gatgcaatt ctggaagag ccagactgg agtacttta 
      gtatcgtat gaggttggc atcgacgag ttaattcac 
      acttttaaa gttcattgc aaaaacaat acgaaaaat 
      tgtgttgaa gtcaagaga ggctatgat gacttgctc 
      gaaacgtca agaaatgca actttacgg aagaagagt 
      tggtccgct tggcagacg ctactttcg accaaattc 
      aacaaattc cactcggtt ttgaatctc attaacaaa 
      gcggctcga ctgaatgaa tttgacgac cctaagtta 
      tactgggag aagttacaa gatctagaa ggcgcgtat 
      aacttagcc gatggtttc tgggaccaa atcaaacca 
      ttttacacg aagttacaa aattacatc aagacccgg 
      cttttgcgt ttttacaaa acaactagt gacgacatc 
      cctgtgtac ctttcgggt tcaaatttt ggggacgac 
      tggagcagc attgccaac ataatccta ccccatcct 
      caaatctat tactatgcg gagtcttac ctcaaccaa 
      acttcaatc aaagaaatt tacaaaatg gccgagcaa 
      gtcactaag gacttggcc ttaggccct ttgggcccc 
      aaattttgg gccaatagc cgcttcaat ttgcccttc 
      tgtgagaac cacgtggtt acgttctgc tcgcaggaa 
      cattccgaa gtgttcact tgcgataaa acatcatgg 
      gtccactat ttaaactca tttgagacg gcaattgac 
      gtggcgtta cgtagcgtc aattatgtt gatttggca 
      agactgaag ttgcgatat tccgccatt gacgacgcc 
      attgttggt ctcggatcg atttttgct atcaagaat 
      ctgcgagtc aaaggagtc tgggactac gaattgttc 
      caaaacaat gataatgaa atgagcgaa cttttatta 
      attgcactg agagttttg ccgaaactt ccgtactat 
      ctggccgcc gataaatgg cggctggaa gtattgcaa 
      aatggtgga ggcgccatg acggccacg tggtggaac 
      ttgcggaag gagttgcaa ggaattggg ggagttttc 
      aacaacgaa accgatttt cttggcgat tcgttcatt 
      gtttcgaat cggccatat ttgagtaaa ttcctcgga 
      accatcttg cagtttcaa ttgctggaa cattaccga 
      aacgggtgg caatatgcc aacgaaagc cttgcggct 
      aatttcgga aacgacgat aatttctta acaatgttg 
      cacgacagg acgtccagt gattggcca gcattgatt 
      aacggtttt ggggtttac gaattgtcg gcgacccca 
      cttttggat tactttgct ccgctaaat gattatctg 
      gacgcgaca ctgaatacg gaacaagtt gtgccttca 
      acgacccca aagcccaca aaagcccag aaaattaaa 
      actcttatt gttaaaaag acaaacgta acagagaca 
      gatgaagat gtgcaaaat gatgaaccg gagcccgaa 
      gttccgaga catattctt gaagatcgt gaagaaact 
      cccaatcac gccatggaa aaaataaca gtttttata 
      ggagtgggg cttttggcg ggtgtcgct gccattata 
      ggatttgtg atacttagg aaaaaaatg acggggaga 
      ggacggacg aataatagg cgctttgag acttgattt 
      ttatgtgta ttaaattat tattttgta ataaaatgt 
      tttaaattt g
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY