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Gene information
General information
TC015230 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 9338795..9340852
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015230
    iBeetle screen iB_02437

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    larva random constricted - Penetrance (percentage of animals/eggs on slide): less than 30%

    head partially decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    procephalic appendages not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    labrum ectopically present - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015230 RA
      MKQPELTIE QGKLLGSTG KDIRGIPFY KFQGIPYAK 
      PPLGHLRFK APEPPEHWE GVLDATKPG NVCYQRDLF 
      RQKITGSEN CLVLNVYTR NIPDGIHTS LYPVLVWIH 
      GGYFIFGSG NDDIYGPEF LLSENLVLV TVNYRLGML 
      GFLCLEDLA LEVPGNAGL KDIVMALKW VQRNIYKFC 
      GNPHNVTIF GQDAGGSSV HLLFLSPLT AGLFHRAIA 
      QSGCAVNPW ARGTRGGNT VAEVLKIGL NDERELLDC 
      LMTKTVEEI LEIQEKIVD KLVASEVRP FGYVLESSA 
      NRFAFITEE PLQAMLGGR FNHVPLIVG FTSSEGMEI 
      YKNLKNQGL PPLETFIPW SFNYNMDKS ETDKLRDRL 
      KKFYFEKEA PLKQVNKIF DLISDSMFI FPIIQTVMI 
      LFHKNKAPT YVYMISAET KLNFYKKLA KIKEPGVSH 
      FDDIGYLFK TFKTPELKP GSVEEVVVT KLIKLWSNF 
      ATFGEPTPH KRDSLLKVK WKPMKKHEI EFLDIGREL 
      KVSAFSAKV PPGNEQSAH F
    CDS sequence
    • >TC015230 RA
      atgaaacaa cccgaatta accatcgag cagggaaaa 
      ttgcttggt tctaccgga aaagacatc cgaggaatc 
      cccttttac aaattccaa ggaatcccc tacgctaaa 
      cccccactt ggccacctc aggtttaag gcccctgaa 
      cctcccgag cactgggag ggagtcctg gacgccacc 
      aagcccgga aatgtatgc taccaacgc gatttgttc 
      cgtcagaaa atcacaggg agcgaaaat tgcctggtt 
      ttgaacgtt tacacgcga aacattcca gacggtatc 
      cacactagc ttgtaccca gtcctggtg tggatacac 
      gggggttat tttattttt gggtcaggg aatgacgat 
      atctacggg cctgagttt ctactaagt gaaaatttg 
      gtcctggtt acggtcaat tatcgacta gggatgttg 
      gggtttttg tgtctcgag gatttggct ttggaggtg 
      cctggaaat gccgggctg aaggacata gtaatggcc 
      ttgaagtgg gtacagagg aatatttat aagttttgt 
      ggaaatcca cacaatgtt acaattttt gggcaagat 
      gctggcggt tccagtgtc catctctta tttttgtcg 
      cctttgacc gctggcctt tttcatcga gcgatcgct 
      cagagtggg tgtgcggtt aatccgtgg gccaggggg 
      accagaggg ggtaacacc gttgctgaa gttctcaaa 
      ataggattg aatgatgaa agggagctt cttgattgt 
      ttgatgaca aaaacagtt gaggaaatt ttggaaatt 
      caagaaaaa atcgttgat aagctggtg gcttccgaa 
      gtcaggcct ttcggttac gttcttgag tcttctgcc 
      aacaggttt gctttcata accgaagaa ccgctacaa 
      gccatgctg gggggaagg tttaaccat gtgcctttg 
      attgttggg tttacaagt tcggaaggg atggagatt 
      tataagaat ttgaaaaat caaggtttg ccacctctt 
      gaaactttt attccttgg agttttaac tacaatatg 
      gataagtct gaaactgat aagttacgt gacaggctt 
      aagaaattt tattttgaa aaggaagct ccgttgaaa 
      caagtcaac aaaattttt gatttgata tctgactca 
      atgtttatt tttccgatc attcaaacc gtaatgatt 
      ttattccac aaaaacaag gcgcccact tatgtctac 
      atgatatca gcagagacc aagctgaat ttttacaaa 
      aagttggcc aaaatcaaa gaacccggt gtttcacat 
      tttgacgat attggttat ctttttaaa acgtttaaa 
      actcctgaa ttgaaacct ggaagtgtg gaagaagtt 
      gtagttacg aaattaata aaactttgg agtaatttt 
      gccacgttt ggagaacca actcctcac aaacgggat 
      tcactgttg aaagtgaag tggaaaccg atgaaaaaa 
      cacgaaatt gagtttctt gacattggg agggagttg 
      aaggtttcg gcattttca gcgaaagtc ccaccggga 
      aacgaacag agtgcccat ttttaa
    mRNA sequence
    • >TC015230 RA
      aacaaataa acaatgcga actttgatt tctcccatt 
      agttaaaaa ttattaaaa tcgtattat aggcgccaa 
      ataaatttg tgttatcac ttgctttgc aaataaata 
      cgactgttt tgacaactt ggcttatca cacagtgag 
      gacccaaat aaaaaacga aagcctaaa agtaggaac 
      aaattgtca aaaattgtc aaaattctt gtaaaatga 
      aacaacccg aattaacca tcgagcagg gaaaattgc 
      ttggttcta ccggaaaag acatccgag gaatcccct 
      tttacaaat tccaaggaa tcccctacg ctaaacccc 
      cacttggcc acctcaggt ttaaggccc ctgaacctc 
      ccgagcact gggagggag tcctggacg ccaccaagc 
      ccggaaatg tatgctacc aacgcgatt tgttccgtc 
      agaaaatca cagggagcg aaaattgcc tggttttga 
      acgtttaca cgcgaaaca ttccagacg gtatccaca 
      ctagcttgt acccagtcc tggtgtgga tacacgggg 
      gttatttta tttttgggt cagggaatg acgatatct 
      acgggcctg agtttctac taagtgaaa atttggtcc 
      tggttacgg tcaattatc gactaggga tgttggggt 
      ttttgtgtc tcgaggatt tggctttgg aggtgcctg 
      gaaatgccg ggctgaagg acatagtaa tggccttga 
      agtgggtac agaggaata tttataagt tttgtggaa 
      atccacaca atgttacaa tttttgggc aagatgctg 
      gcggttcca gtgtccatc tcttatttt tgtcgcctt 
      tgaccgctg gcctttttc atcgagcga tcgctcaga 
      gtgggtgtg cggttaatc cgtgggcca gggggacca 
      gagggggta acaccgttg ctgaagttc tcaaaatag 
      gattgaatg atgaaaggg agcttcttg attgtttga 
      tgacaaaaa cagttgagg aaattttgg aaattcaag 
      aaaaaatcg ttgataagc tggtggctt ccgaagtca 
      ggcctttcg gttacgttc ttgagtctt ctgccaaca 
      ggtttgctt tcataaccg aagaaccgc tacaagcca 
      tgctggggg gaaggttta accatgtgc ctttgattg 
      ttgggttta caagttcgg aagggatgg agatttata 
      agaatttga aaaatcaag gtttgccac ctcttgaaa 
      cttttattc cttggagtt ttaactaca atatggata 
      agtctgaaa ctgataagt tacgtgaca ggcttaaga 
      aattttatt ttgaaaagg aagctccgt tgaaacaag 
      tcaacaaaa tttttgatt tgatatctg actcaatgt 
      ttatttttc cgatcattc aaaccgtaa tgattttat 
      tccacaaaa acaaggcgc ccacttatg tctacatga 
      tatcagcag agaccaagc tgaattttt acaaaaagt 
      tggccaaaa tcaaagaac ccggtgttt cacattttg 
      acgatattg gttatcttt ttaaaacgt ttaaaactc 
      ctgaattga aacctggaa gtgtggaag aagttgtag 
      ttacgaaat taataaaac tttggagta attttgcca 
      cgtttggag aaccaactc ctcacaaac gggattcac 
      tgttgaaag tgaagtgga aaccgatga aaaaacacg 
      aaattgagt ttcttgaca ttgggaggg agttgaagg 
      tttcggcat tttcagcga aagtcccac cgggaaacg 
      aacagagtg cccattttt aaagcaact attatttaa 
      aaaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY